Die vorliegende Studie wurde in einer Gruppe von Dörfern im Ostsudan, Gedaref State, durchgeführt. Bei 100 Patienten mit der Verdachtsdiagnose Kala Azar- oder Post-Kala Azar- Leishmaniose war der Erregernachweis mit der PCR direkt in klinischen Proben, die auf Filterpapier aufgebracht worden waren, ohne vorherige Kultivierung erfolgreich. In dieser PCR wurden die ribosomalen, internal transcribed spacer (ITS1 & ITS2) amplifiziert, weil sie sehr variabel sind, eine klare Speziesidentifizierung gestatten und bei weiterführenden Analysen der PCR-Produkte auch der Nachweis stammspezifischer Unterschiede erwartet werden konnte. Für die Analyse der Diversität von Leishmania donovani-Isolaten aus dem Sudan wurden 4 verschiedene PCR-basierte Methoden eingesetzt: das PCR-Fingerprinting mit unspezifischen Einzelprimern, die RFLP- Analyse des amplifizierten ITS-Locus, ,,single strand conformation polymorphism (SSCP)- Analysen der amplifizierten ITS-Region, des Gens, welches für die Hauptoberflächenprotease (gp63) kodiert, und anonymer DNA- Fragmente sowie Sequenzanalysen der entsprechenden Zielregionen. Das PCR-Fingerprinting und die Restriktionsanalyse der ITS-Region lieferten weitgehend übereinstimmende Fragmentmuster für alle untersuchten L.donovani-Stämme. 12 unterschiedliche Profile wurden bei der SSCP-Analyse des ITS1-Locus für 86 Isolate aus dem Sudan erhalten, während der ITS2-Locus bei diesen Stämmen hochkonserviert war und nur ein Stamm ein unterschiedliches SSCP-Muster aufwies. L. Donovani -Stämme anderer geographischer Herkunft hatten unterschiedliche ITS2-Profile in der SSCP. Für den gp63 - Locus waren 3 polymorphe SSCP-Muster bei 31 untersuchten sudanesischen Isolaten nachweisbar. Für die meisten der anonymen DNA-Fragmente, L510, L413, LK413, L0308 UND L0114, konnten leider nur von 8 kultivierten Stämmen gute PCR-Produkte erhalten werden. Lediglich das Fragment L0110 konnte erfolgreich von 31 auf Filterpapier aufgebrachten Proben direkt amplifiziert werden. Die Suche nach Polymorphismen mit der SSCP ergab keine Unterschiede in diesen anonymen DNA-Regionen, mit Ausnahme des Fragments L0114, das zwei verschiedene Muster aufwies. Die Ergebnisse der SSCP-Analysen und der DNA- Sequenzierung stimmten gut überein, wodurch bestätigt wurde, dass die SSCP genetische Unterschiede auf dem Niveau einzelner Basenaustausche nachweisen kann. Die SSCP- Technik hat Vorteile gegenüber den anderen Methoden, die für die Untersuchung von Sequenzvariationen innerhalb der Spezies L. donovani angewandt wurden. Es konnten keine Korrelationen zwischen der Form der klinischen Manifestation und den Ergebnissen des PCR- Fingerprinting, der ITS-RFLP- und ITS-SSCP- Analysen festgestellt warden. Diese Studie ist von besonderem Nutzen in epidemiologischen Feldstudien, bei denen die Kultivierung der Erreger besonders in Entwicklungsländern extrem schwierig sein kann. / This study was carried out in clusters of villages that represent an endemic focus of visceral leishmaniasis (VL). These villages were located in Gedaref state, eastern Sudan. For diagnostic purposes polymerase chain reaction (PCR) was performed successfully, directly from clinical samples spotted on filter papers with no prior cultivation from 100 patients suspected of having kala-azar or post kala-azar dermal leishmaniasis. Mainly the ribosomal internal transcribed spacer (ITS1 & ITS2) were targeted in PCR because this region is more variable and allows clear species identification and also strain differences could be expected by further analysis of these PCR products. PCR was found to be more sensitive compared to the gold standard microscopic method. Four PCR based approaches were used to analyse diversity within Sudanese isolates of Leishmania donovani. Methods compared were fingerprinting with single non-specific primers, restriction analysis of the amplified ITS locus (RFLP), single-stranded conformation polymorphism (SSCP) of the ITS region, major surface protease (gp63) gene, anonymous DNA fragments and sequencing of these targeted regions. When PCR fingerprinting and restriction analysis of ITS region were applied, highly similar fragment patterns were observed for all strains of L. donovani studied. The ITS1 locus gave 12 different SSCP profiles among the 86 Sudanese isolates, where as the ITS2 locus was highly conserved among the 86 samples with the exception of 1 isolate. Strains of L. donovani derived from other geographical areas were found to have different ITS2 patterns. The gp63 locus gave 3 polymorphic patterns among 31 Sudanese isolates. Concerning most of the anonymous DNA fragments namely, L510, L413, LK413, L0308 and L0114 unfortunately, we succeeded to get good PCR products only from DNA extracted 8 successful cultures. Only for the fragment L0110 we were able to get good PCR products from 31 samples spotted on filter papers. When these PCR products were investigated for polymorphisms using SSCP no differences were observed with exception of L0114 region, which showed 2 patterns. SSCP analysis correlates well with results of DNA sequencing and confirmed that SSCP was able to detect genetic diversity at the level of a single nucleotide. SSCP had advantages over the other methods employed for investigating of sequence variation within the species L. donovani. There was no correlation between the form of clinical manifestation of the disease and the PCR fingerprinting, ITS-RFLP, or ITS-SSCP characteristics. This study is beneficial particularly in epidemiological studies based on field-work where obtaining cultures can be extremely difficult especially in developing countries.

Determination of Parenthood (Abstract) The determination of parenthood is undoubtedly a very topical issue. There are significant changes which are consequences of modern society approach to the family life as well as of massive progress in the field of medical science and genetics. The development in these areas has brought new concepts such as baby-boxes, assisted reproduction and surrogacy. Now it is up to the legislation to provide rules within the legal framework for these new institutions to steady parental relationships and to secure their protection as well. The aim of this thesis is to familiarize the reader with Czech legal regulation of determination of parenthood by means of its description and analysis. Another aim of the work is to compare this legal regulation with regulation in other countries, in particular in Germany and to find similarities and differences between them. Finally the thesis highlights new institutions within determination of parenthood. This thesis consists of nine chapters which deal with particular parts of its subject-matter. The opening chapter discusses the relationship between a parent and a child both theoretically and historically. Apart from that it also describes legal regulations applicable in the Czech Republic and Germany in the course of time. The second...

Determination of Parenthood (Abstract) The topic determination of parenthood today is very actual nowadays and it is not possible to neglect its importance. Changes taking place both in the social life of modern times, and in the field of medicine is necessary to constantly reflect and to adapt them to the legal order. Concepts such as assisted reproduction, surrogacy, concealed birth and baby-boxes are a common part of human society and it is not possible to avoid dealing with the situation around them. The aim of this thesis is to summarize the Czech legislation of determination of parenthood, to mention problematic situations that may arise in connection with it and describe possible solutions to these situations. The another aim then is to acquaint the reader with the newly developing institutions, such as assisted reproduction, surrogacy, concealed birth, baby boxes, etc., and analysis of their current legal status supplemented by comparison of legislation in other European and non-European countries, which could serve as inspiration for future development in the Czech legal order. The first chapter deals with family law, family and relationships between parents and children both from a theoretical and historical point of view. The second chapter is devoted to an overview of the most important changes...

R I G H T T O R E S P E C T F O R P R I V A T E A N D F A M I L Y L I F E I N T H E C A S E - L A W O F T H E E U R O P E A N C O U R T O F H U M A N R I G H T S Abstract The aim of the thesis is to explain wording and application of the Article 8 of the European Convention for the Protection of Human Rights and Fundamental Freedoms (the Convention) from the perspective of the European Court of Human Rights (the ECHR). The scope of Article 8, which guarantees the right to respect for private and family life, is very broad. For this reason the thesis especially deals with the issue of the right of the child to know his/her origin and information about his/her early childhood. In particular it attempts to outline the way how the ECHR deals with the conflict of the right to know one's origin with the right of the parent to stay anonymous. The thesis is divided into five chapters which illustrate the particular aspects of the application of Article 8. These chapters are linked through the judgements Gaskin, Mikulić, Odiévre, Jäggi and Phinirikadou which are related to the right of the child to know his/her origin. Chapter 1 provides with the general introduction to the protection of human rights at the European level and also analyses the Convention and the ECHR. The goal of chapter 2 is to outline the inner...

- Determination of Parenthood The topic of my thesis is Determination of Parenthood. With this topic are connected problems not only legal, but often also social and ethical. Parenthood affects all of us and it's an important aspect of human life. That's why there should be legal assurance in relationship between parent and child. Development in the field of medicine is still ahead (mainly thanks to methods of assisted reproduction and DNA analysis) and the rules of law should respond and deal with it. The aim of this thesis is describe and become acquainted with problematics of determination of parenthood, valorize how legislation responds to modern development and to propose possible changes. Thesis is prepared on the basis of existing legislation on the date of submission of the thesis. Thesis is divided into seven main chapters, which are divided to further subsections. First chapter deals with concept of parenthood and methods of determination. Second chapter describes historical development of legislation on Czech territory, since Roman law to recodification of civil law. Related third chapter concisely summarizes national and international legal sources of current legislation. Chapter four is about determination of maternity and problems of surrogacy, anonymous childbirth, concealed birth...

Desde a metade do século XX os grupos de ajuda mútua de Narcóticos Anônimos vêm se firmando como uma alternativa terapêutica válida para pessoas que desenvolvem a síndrome de "dependência química". Surgidos como uma dissidência dos Alcoólicos Anônimos, estes grupos utilizam de maneira pragmática o conceito de "adicção" para nomear esta doença e classificam-na como incurável, sendo possível apenas o controle dos sintomas. Desta forma, a sua proposta terapêutica é pautada na abstinência total do uso de qualquer substância psicoativa e em um conjunto de princípios éticos, espirituais e subjetivos que devem ser seguidos pelos participantes com o objetivo de "vivenciarem a recuperação" através de uma mudança radical de visão de mundo. Este trabalho tem como universo de pesquisa a rede de Narcóticos Anônimos da cidade de Porto Alegre. Dentro deste universo empírico um dos objetivos da pesquisa é demonstrar, através da apresentação de quatro histórias de vida de participantes dessa rede, que a categoria "membro de Narcóticos Anônimos" é menos homogênea do que aparenta ser; há espaço para a apropriação instrumental deste universo simbólico e também para a preservação de particularidades subjetivas no processo de incorporação dessa identidade. Outro objetivo é apresentar as implicações da utilização do seu conceito próprio de "adicção" enquanto doença incurável. Finalmente, através de um relato etnográfico, procura apresentar as reuniões do grupo como um espaço marcadamente ritual, mas que preserva momentos de interação e difusão de símbolos também "fora do ritual". / Since the half of the twentieth century mutual help groups such as Narcotics Anonymous have been establishing themselves as a valid therapeutic alternative for people who develop the "chemical dependence" syndrome. Emerging as a dissidence of Alcoholics Anonymous, these groups apply a pragmatic interpretation of the "addiction" concept to name that disease and classify it as incurable; they say that only the symptoms can be controlled. This way, their therapeutic approach is based on the total abstinence of any psychoactive substance use and in a set of ethical, spiritual and subjective principles that must be followed by the members with the objective of "living the recovery" through a radical change of subjective engagement in the world. This work's universe of research is the Narcotics Anonymous' network in the city of Porto Alegre. Inside this empirical universe one of the research's objectives is to demonstrate, by the presentation of four life stories of this networks' participants, that the category "Narcotics Anonymous' member" is less homogeny than it apparent to be; there is some space to instrumental appropriation of this symbolic universe and also to the preservation of subjective particularities in the process of incorporating this identity. Another objective is to discuss the implications of using the "addiction" category as a synonymous of incurable disease. Finally, this research presents an ethnographic description of the groups' meetings as composed by a rigid ritual structure, but which also preserves moments of interaction and symbol dissemination that lie "outside from the ritual".

Desde a metade do século XX os grupos de ajuda mútua de Narcóticos Anônimos vêm se firmando como uma alternativa terapêutica válida para pessoas que desenvolvem a síndrome de "dependência química". Surgidos como uma dissidência dos Alcoólicos Anônimos, estes grupos utilizam de maneira pragmática o conceito de "adicção" para nomear esta doença e classificam-na como incurável, sendo possível apenas o controle dos sintomas. Desta forma, a sua proposta terapêutica é pautada na abstinência total do uso de qualquer substância psicoativa e em um conjunto de princípios éticos, espirituais e subjetivos que devem ser seguidos pelos participantes com o objetivo de "vivenciarem a recuperação" através de uma mudança radical de visão de mundo. Este trabalho tem como universo de pesquisa a rede de Narcóticos Anônimos da cidade de Porto Alegre. Dentro deste universo empírico um dos objetivos da pesquisa é demonstrar, através da apresentação de quatro histórias de vida de participantes dessa rede, que a categoria "membro de Narcóticos Anônimos" é menos homogênea do que aparenta ser; há espaço para a apropriação instrumental deste universo simbólico e também para a preservação de particularidades subjetivas no processo de incorporação dessa identidade. Outro objetivo é apresentar as implicações da utilização do seu conceito próprio de "adicção" enquanto doença incurável. Finalmente, através de um relato etnográfico, procura apresentar as reuniões do grupo como um espaço marcadamente ritual, mas que preserva momentos de interação e difusão de símbolos também "fora do ritual". / Since the half of the twentieth century mutual help groups such as Narcotics Anonymous have been establishing themselves as a valid therapeutic alternative for people who develop the "chemical dependence" syndrome. Emerging as a dissidence of Alcoholics Anonymous, these groups apply a pragmatic interpretation of the "addiction" concept to name that disease and classify it as incurable; they say that only the symptoms can be controlled. This way, their therapeutic approach is based on the total abstinence of any psychoactive substance use and in a set of ethical, spiritual and subjective principles that must be followed by the members with the objective of "living the recovery" through a radical change of subjective engagement in the world. This work's universe of research is the Narcotics Anonymous' network in the city of Porto Alegre. Inside this empirical universe one of the research's objectives is to demonstrate, by the presentation of four life stories of this networks' participants, that the category "Narcotics Anonymous' member" is less homogeny than it apparent to be; there is some space to instrumental appropriation of this symbolic universe and also to the preservation of subjective particularities in the process of incorporating this identity. Another objective is to discuss the implications of using the "addiction" category as a synonymous of incurable disease. Finally, this research presents an ethnographic description of the groups' meetings as composed by a rigid ritual structure, but which also preserves moments of interaction and symbol dissemination that lie "outside from the ritual".

The MA thesis compares cognitive-behavioral, spiritual and holotropic breathwork approaches in drug abuse treatment. The theoretical part briefly describes a function of some psychotropic substances and drugs in history. It follows the description of drug addiction genesis and drug addiction types. Other chapter of the theoretical part deals with system of treatment for drug addicts and role of psychotherapy in the treatment. There is also a description of three fundamental approaches for three methods used in drug abuse treatment at the end of the theoretical part. The practical part of the MA thesis characterizes the comparison of three institutions, which use different methods in drug abuse treatment. The chosen institutions were: cognitive-behavioral method {--} Červený Dvůr Psychiatric Hospital, spiritual approach {--} Postovice Therapeutic Community, holotropic breathwork method {--} Atelier II Private Psychotherapeutic Centre. Although, each of these institutions provides a treatment for substance (alcohol or other psychotropic drugs) abuse and addiction, their methods / approaches to the treatment have different fundamental bases. This part also contains transcriptions of structured talks with clients who experienced the mentioned methods / approaches. At the end of the MA thesis the selected approaches based on monitored marks are analyzed and compromise approach for drug abuse treatment is suggested.

Desde a metade do século XX os grupos de ajuda mútua de Narcóticos Anônimos vêm se firmando como uma alternativa terapêutica válida para pessoas que desenvolvem a síndrome de "dependência química". Surgidos como uma dissidência dos Alcoólicos Anônimos, estes grupos utilizam de maneira pragmática o conceito de "adicção" para nomear esta doença e classificam-na como incurável, sendo possível apenas o controle dos sintomas. Desta forma, a sua proposta terapêutica é pautada na abstinência total do uso de qualquer substância psicoativa e em um conjunto de princípios éticos, espirituais e subjetivos que devem ser seguidos pelos participantes com o objetivo de "vivenciarem a recuperação" através de uma mudança radical de visão de mundo. Este trabalho tem como universo de pesquisa a rede de Narcóticos Anônimos da cidade de Porto Alegre. Dentro deste universo empírico um dos objetivos da pesquisa é demonstrar, através da apresentação de quatro histórias de vida de participantes dessa rede, que a categoria "membro de Narcóticos Anônimos" é menos homogênea do que aparenta ser; há espaço para a apropriação instrumental deste universo simbólico e também para a preservação de particularidades subjetivas no processo de incorporação dessa identidade. Outro objetivo é apresentar as implicações da utilização do seu conceito próprio de "adicção" enquanto doença incurável. Finalmente, através de um relato etnográfico, procura apresentar as reuniões do grupo como um espaço marcadamente ritual, mas que preserva momentos de interação e difusão de símbolos também "fora do ritual". / Since the half of the twentieth century mutual help groups such as Narcotics Anonymous have been establishing themselves as a valid therapeutic alternative for people who develop the "chemical dependence" syndrome. Emerging as a dissidence of Alcoholics Anonymous, these groups apply a pragmatic interpretation of the "addiction" concept to name that disease and classify it as incurable; they say that only the symptoms can be controlled. This way, their therapeutic approach is based on the total abstinence of any psychoactive substance use and in a set of ethical, spiritual and subjective principles that must be followed by the members with the objective of "living the recovery" through a radical change of subjective engagement in the world. This work's universe of research is the Narcotics Anonymous' network in the city of Porto Alegre. Inside this empirical universe one of the research's objectives is to demonstrate, by the presentation of four life stories of this networks' participants, that the category "Narcotics Anonymous' member" is less homogeny than it apparent to be; there is some space to instrumental appropriation of this symbolic universe and also to the preservation of subjective particularities in the process of incorporating this identity. Another objective is to discuss the implications of using the "addiction" category as a synonymous of incurable disease. Finally, this research presents an ethnographic description of the groups' meetings as composed by a rigid ritual structure, but which also preserves moments of interaction and symbol dissemination that lie "outside from the ritual".

(Abstract) The diploma thesis deals with the reflection of the right to know one's own origin in the Czech legal order. Knowledge of one's own origin and the right to know one's own origin constitute not only a legal but also a moral and ethical question. The right to know one's own origin (i.e. the right to know one's parents, one's biological family and one's ancestors) is one of the subjective human rights, which belongs to every natural person. This right is more or less reflected in majority of institutes of family law. The thesis analyses the most important of them and indicates how the right to know one's own origin is reflected in these institutes or it is not, although it should be. The work seeks to highlight possible shortcomings of the legislation and tries to propose possible solutions de lege ferenda. There are also stated (for the purposes of comparison) some examples of foreign legislation in cases of some legal institutes. The diploma thesis is based on the legislation effective on the 26th March 2016. It is composed of an introduction, five chapters and a conclusion. The first chapter deals with the right to know one's own origin and its legal regulation in the Czech Republic. It mainly discusses the international conventions that are (in compliance with the Art. 10 of the...