A presente pesquisa tem como objetivo compreender a produção do espaço e de suas representações no bairro Restinga - Porto Alegre/RS. São construções atravessados por materialidades, idéias, intenções e vidas, que formam, de forma contraditória, complexa e singular, o bairro. Sua produção material é indissociável de seus imaginários e, embora o espaço seja abstração, é também materialidade na vida das pessoas, envolvendo desde a ordem distante, do Estado e instituições, até a ordem próxima, dada pelas relações em redes ou de vizinhança. Nesse sentido, a produção do espaço é acompanhada por distintas (e contraditórias) representações, constituídas dentro e fora da Restinga. São imaginários que originaram com a produção do bairro e a remoção das malocas, reproduzindo hoje, sob outras roupagens, as distâncias sociais. Contudo, o morador que encontra no bairro o abrigo dentro de uma sociedade desigual, constitui valores positivos, dissociando-o dos imaginários que o estigmatizam. É nesse sentido, que fatos e idéias compõem o viver do morador da periferia, constituindo os vínculos entre sujeito e bairro, em elaborações constantes do espaço de representação. / This research aims to understand the production of space and its representations at Restinga neighborhood, in Porto Alegre, RS. They are constructions built by materialities, ideas, intentions and lives, which form, in a contradictory, complex and singular way, this neighborhood. Its material production is not separable from its imaginary and, although the space is abstraction, it is also materiality in people's lives, evolving from the distant order - State and institutions - to the nearer order, given by the relationships of neighborhood or in nets. Thus, the production of space is kept up with distinct (and contradictory) representations, formed inside and outside Restinga. These imaginaries were created with the production of the neighborhood and the removing of the poorest handmade houses, which reproduces, in another way, the social distances. However, the person who finds in the neighborhood a shelter inside an unequal society has positive values, undoing it from the imaginaries that stigmatize it. Facts and ideas compound the people from outskirts' lives, which build the links between individual and neighborhood, in constant elaborations of space of representation.

Orientador: Antonio Carlos Paes / Banca: Jane Megid / Banca: Márcio Garcia Ribeiro / Banca: Sandra de Moraes Gimenes Bosco / Banca: Rogério Giuffrida / Resumo: O presente estudo teve por objetivos determinar a ocorrência de Streptococcus suis em funcionários (portadores sadios) e ambiente de abatedouros com SIF do estado de São Paulo, Brasil, descrever a distribuição dos diferentes tipos do agente e o perfil de suscetibilidade aos antimicrobianos das linhagens isoladas, bem como pesquisar os fatores de risco associados à ocorrência do patógeno. Suabes de tonsilas de 139 funcionários e 261 suabes ambientais foram coletados para detecção de S. suis e seus tipos através das técnicas de monoplex e multiplex PCR, respectivamente. Não houve detecção de funcionários portadores sadios. S. suis foi isolado de 27,03% das amostras coletadas no abatedouro A; 17,31% no abatedouro B; 15,31% no abatedouro C; e 36,36% no abatedouro D. Diferença estatisticamente significativa (p<0,05) foi observada para as ocorrências de S. suis entre os abatedouros e entre as áreas de risco baixo, médio e alto da etapa de matança no abatedouro A. Os tipos 4 (20,00%), 5 (20,00%), 12 (20,00%) e 29 (20,00%) foram os mais frequentes seguidos pelos tipos 21 (10,00%) e 31 (10,00%). Os antimicrobianos mais eficazes foram amoxicilina com clavulanato (100,00%), doxiciclina (100,00%), ceftiofur (93,75%), ampicilina (81,25%) e cefalexina (75,00%). Multirresistência foi observada em 100,00% das linhagens isoladas. Os abatedouros e as diferentes áreas da etapa de matança podem ser fatores de risco para a ocorrência de S. suis, consequentemente, para infecção humana pelo patógeno. No entanto, é importante ressaltar que todos os funcionários estão expostos à infecção. O presente trabalho é o primeiro estudo epidemiológico realizado no Brasil acerca dos fatores de risco associados à ocorrência de S. suis envolvendo grande número de indivíduos pertencentes ao principal grupo de risco / Abstract: The present study aimed to determine the occurrence of Streptococcus suis in employees (healthy carriers) and environment of swine slaughterhouses with federal inspection service of the state of São Paulo, Brazil, to describe the distribution of the different types of the agent and the antimicrobial susceptibility profile of the isolated strains, as well as to research the risk factors associated with the occurrence of the pathogen. Tonsillar swabs of 139 employees and 261 environmental swabs were collected for detection of S. suis and its types by monoplex and multiplex PCR, respectively. There was no detection of healthy carriers employees. S. suis was isolated from 27.03% of the samples collected at slaughterhouse A; 17.31% at slaughterhouse B; 15.31% at slaughterhouse C; and 36.36% at slaughterhouse D. Significant statistical differences (p<0.05) were observed for the occurrence of S. suis among slaughterhouses and among areas of low, medium and high risk of the slaughterhouse A. The types 4 (20.00%), 5 (20.00%), 12 (20.00%) and 29 (20.00%) were the most frequent followed by types 21 (10.00%) and 31 (10.00%). The most effective antimicrobials were amoxicillin-clavulanate (100.00%), doxycycline (100.00%), ceftiofur (93.75%), ampicillin (81.25%) and cephalexin (75.00%). Multidrug resistance was observed in 100.00% of the isolates. The slaughterhouses and the different areas of the pig slaughtering may be risk factors for the occurrence of S. suis and, consequently, for human infection. However, it is noteworthy that all employees are exposed to S. suis infection. This project is the first epidemiological study in Brazil about the risk factors associated with the occurrence of S. suis involving a large numbers of individuals belonging to the main risk group / Doutor

Thesis (MTech (Chemical Engineering))--Cape Peninsula University of Technology, 2017. / The poultry industry is one of the largest industries in the South African agricultural sector. To sustain their various operations, this industry utilises a large quantity of potable water to process slaughtered birds in order to satisfy hygiene and sanitation requirements in processing facilities. Thus, the consumption of potable water during poultry slaughterhouse operations results in the production of high-strength poultry slaughterhouse wastewater (PSW), which is laden with a variety of pollutants, including fats, oil and grease (FOG), carcass debris, feathers and organic matter, including proteins, that should be removed from the wastewater, or at least reduced in concentration, prior to the PSW being discharged into the environment. This is to avoid and/or minimise levies and non-compliance penalties from monitoring institutions in charge of controlling the quality of effluents in the area from which the PSW was collected for this study. Furthermore, the option of treating and recycling the PSW to address the current issue of water scarcity in the Western Cape (South Africa), and to minimise possible harmful effects on the environment, will reduce the overreliance on slaughterhouses in the region on potable/drinking water, thus also lessening running costs associated with water procurement for operations. Various technologies, involving physical, chemical or biological processes, have been evaluated for the treatment of PSW, with this study focusing on anaerobic treatment (part of the biological treatment) of PSW, using a high-rate anaerobic bioreactor system (HRABs), which provides for low production of sludge, the production of biogas as a source of energy and the provision of high performance in terms of organic matter removal. Moreover, HRABs are cheaper, when compared to other aerobic treatment technologies. However, numerous potential challenges were encountered when using HRABs, such as low production of biogas due to gas entrapment, head losses across the granular bed, sludge washout in upflow HRABs, uneven wastewater distribution, and thus poor dispersion of the organic matter, which impacts on the adequacy of treatment, poor release of toxic substances contained in the entrapped biogas (NH3 or H2S), clogging of the underdrain system for down-flow HRABs, or the formation of dead zones within the granular bed, resulting in short-circuiting.

Thesis (DTech (Chemical Engineering))--Cape Peninsula University of Technology, 2017. / The process of anaerobic digestion (AD) is one of the most cost-effective and environmentally sustainable technologies to treat wastewater in the agricultural sector. In South Africa, in some industries in the agricultural sector, such as the poultry industry in particular, slaughterhouses have the highest consumption of potable water, culminating in the production of a large quantity of high strength wastewater. This high consumption of potable water has become a concern in South Africa due to water scarcity and reduced rainfall attributed to global warming, including weather changes. Furthermore, the generation of a large volume of wastewater poses environmental pollution concerns. The wastewater from poultry slaughterhouses can be quite easily treated to a suitable quality for reuse, using various bioreactor systems that utilise low cost anaerobic digestion processes. However, as this wastewater contains a high quantity of biodegradable organic matter – with the primary pollutants being proteins, blood, fats, oil and grease (FOG) – selecting a suitable anaerobic reactor configuration (up-flow vs down-flow) plays an important role in achieving high reactor performance. In this study, both the up-flow, (i.e. Expanded Granular Sludge Bed Reactor) and the down-flow (i.e. Static Granular Static Granular Bed Reactor), were studied to quantitatively determine their performance in treating poultry slaughterhouse wastewater. Firstly, the feasibility of treating poultry slaughterhouse wastewater with an up-flow Expanded Granular Sludge Bed Reactor (EGSB) coupled with anoxic and aerobic bioreactors was investigated at an HRT of 7 (168 hr), 4 (96 hr) and 3 (72 hr) days using organic loading rates of 0.5, 0.7 and 1.0 gCOD/L.day. The averaged tCOD removal for the EGSB reactor was 40%, 57% and 55%, respectively, at the various OLRs and HRTs investigated. The overall tCOD removal of the system (EGSB-anoxic/aerobic) at high OLR of 1.0 gCOD/L.day was increased to 65%. The redundant performance of the up-flow EGSB reactor was attributed to the periodical sludge washout experienced during its operation due to high FOG and TSS concentrations in the influent. Due to the periodic sludge washout, the reactor required continuous re-inoculation resulting in the EGSB being operated for a short period (i.e. 26 days). As a result of such system deficiency, it was recommended that to improve the performance of the up-flow EGSB reactor in treating poultry slaughterhouse wastewater, a pre-treatment system – such as a Dissolved Air Floatation system (DAFs) or a FOG skimmer – is required to reduce the FOG and total suspended solids (TSS) load prior to the wastewater fed to the EGSB. This will minimise system failure and the need for a continuous re-inoculation of the system (see Appendix C for improved operation strategy of the EGSB reactor). Furthermore, a system redesign was recommended, thus the use of the SGBR. Secondly, after the EGSB system evaluation, the performance of a down-flow system (i.e. SGBR) for the new design, the following were deemed appropriate for improved system (SGBR) design: 1) reduced HRT for high wastewater treatment through-put rates; 2) the ability to adequately treat the wastewater with higher organic loading rates; and 3) reduction of the plant footprint by using a membrane filtration system (i.e. a single process unit) to effectively reduce process requirements needed for the anoxic/aerobic bioreactors (i.e. n=2 process unit) used with the EGSB. Similarly, for large-scale operations, it is advisable to have a backwash system to adequately handle declogging processes (i.e. these systems modifications were evaluated in the SGBR). The SGBR, coupled with an ultra-filtration (UF) membrane system, was then investigated for treating the poultry slaughterhouse wastewater at an HRT of 55 hrs and 40 hrs, including average OLRs of 1.01 and 3.14 gCOD/L.day, respectively. The average maximum performance of the SGBR in terms of tCOD, TSS and FOG removal was > 90% at the OLRs and HRTs investigated. The UF membrane system used as a post-treatment system further yielded a system performance improvement for tCOD, TSS and FOG of 64%, 88% and 60%, respectively. The overall performance of the combined system (SGBR and UF membrane system) in terms of tCOD, TSS and FOG removal was 98%, 99.8% and 92.4%, respectively. The highest performance for the down-flow SGBR was attributed to its ability to retain granulated sludge in the reactor while maximizing the digestion of the organic matter fed into the reactor, even at higher OLRs. Furthermore, for effective declogging, the implementation of a periodic backwash system to effectively remove dispersed fine sludge particles in the underdrain and excessive suspended solids entrapment was observed to ease the system operational deficiencies.

Thesis (MTech (Construction Management))--Cape Peninsula University of Technology, 2017. / The supply of housing products that is durable, obtained at optimum cost and available within the shortest time possible describes the term sustainable housing. Findings derived from the reviewed literature concerning the ever-increasing quest of construction clients for ‘best value for money’ on construction projects brought about the emergence of alternative contract procurement strategies. Essentially, literature revealed that it is vital to adopt an appropriate contract procurement strategy, one that best addresses particular project needs and objectives, as the adoption of an inappropriate procurement system would result in excessive project cost and time overrun, low project quality standards and the dissatisfaction of involved construction stakeholders. The quest for the supply of sustainable housing in the Western Cape, South Africa, prompted the need to establish an effective contract procurement strategy for housing project delivery. Noteworthy, this study identified five (5) objectives directed towards establishing an effective contract procurement strategy to enhance sustainable housing delivery: 1) the first objective examined the effectiveness of contract procurement strategies used for housing projects; 2) the second objective identified the factors influencing the selection of a suitable contract procurement strategy for housing projects; 3) the third objective analysed the influence of contract procurement strategies on project cost, time and quality; 4) the fourth objective examined the benefits and shortfalls associated with various contract procurement strategies on housing project resources – construction materials, machinery and manpower; 5) and the last objective was to establish the contract procurement strategy that is most effective for the delivery of sustainable housing. This research adopted a mixed methodological approach involving the administration of close-ended quantitative questionnaires submitted to construction professionals and stakeholders and semi-structured qualitative interviews conducted with construction site managers and supervisors. SPSS version 24 software was used to analyse the quantitative data elicited, and “content analysis” was used to analyse the information obtained through the qualitative interviews. Cronbach’s alpha coefficient reliability test was conducted on scaled research questions to ensure the reliability of the research questionnaire. The research findings revealed that the traditional and the design and build contract procurement approaches are cost, time and quality effective and therefore satisfactory, while the traditional contract procurement system is also effective regarding construction stakeholder satisfaction. The research identified that the significant factors influencing the selection of a contract procurement strategy include delay and mistakes in producing design documents; client inability to brief and make timely decisions; project type, nature, scope and complexity; lack of communication and feedback; lack of discipline among construction workers; and finally, lack of availability of construction materials. The study concluded that thorough consideration of these findings by construction stakeholders within the construction industry will enhance the delivery of affordable, sustainable housing. The research study recommends, among other issues, the comparison of effectiveness of contract procurement strategies on project performance on a site-by-site basis, through work study and other available strategies, to further enhance sustainable housing delivery in the South African construction industry.

Thesis (MTech (Chemical Engineering))--Cape Peninsula University of Technology, 2017. / In the recent past, the poultry industry in South Africa has grown due to an increased demand of poultry products as a result of population growth and improved living standards. Furthermore, this has led to poultry slaughterhouses generating high strength wastewater which is laden with a high concentration of organic and inorganic pollutants from the slaughtering process and sanitation of equipment and facilities. As a result, South Africa has promulgated restrictions and a set of quality standards for effluent discharged into the environment to minimize ecological degradation and human health impact. Hence, there is a need for improved Poultry Slaughterhouse Wastewater (PSW) pre-treatment prior to either discharge into municipal wastewater treatment plants (WWTP) or on-site secondary treatment processes such as anaerobic digesters. Additionally, amongst the pre-treatment methods for Fats, Oil and Grease (FOG) laden wastewater, flotation remains the most popular with Dissolved Air Flotation (DAF) system being the most applied. However, modelling and optimization of a biological DAF system has never been attempted before in particular for a bioflocculant supported DAF (BioDAF) for PSW pre-treatment. Process modelling and optimization involves process adjustment to optimize influential parameters. In this study, Response Surface Methodology (RSM) was used to develop an empirical model of a BioDAF for pre-treatment of PSW, for which a bioflocculant producer including production conditions, flocculant type and its floc formation mechanism, were identified. Twenty-one (n = 21) microbial strains were isolated from the PSW and their flocculation activity using kaolin clay suspension (4g/L) was quantified, with a mutated Escherichia coli (mE.coli) [accession number LT906474.1], having the highest flocculation activity even in limited nutrient conditions; hence, it was used for further analysis in other experiments. Furthermore, the optimum conditions for bioflocculant production achieved using RSM were pH of 6.5 and 36°C conditions which induced instantaneous bioflocculant production with the highest flocculation activity. The bioflocculant produced by the mE.coli showed the presence of carboxyl/amine, alkyne and hydroxyl functional groups, which was indicative that the bioflocculant contained both polysaccharides and some amino acids.

Orientador: Helio Langoni / Banca: Antonio Carlos Paes / Banca: Márcio Garcia Ribeiro / Banca: Lisiane de Almeida Martins / Banca: Luiz Fernando Pita Gondim / Resumo: Toxoplasma gondii é um parasito de grande importância no contexto de produção animal e saúde pública, sendo importante patógeno oportunista em pacientes imunocomprometidos. Este estudo objetivou determinar a soroprevalência de toxoplasmose em ovinos de abatedouros do estado de São Paulo, e a variabilidade genotípica dos isolamentos obtidos. A presença de anticorpos para T. gondii foi pesquisada em 602 amostras de soro pela reação de imunofluorescência indireta (RIFI), aglutinação direta modificada (MAT), com antígeno fixado pelo metanol (AM) e formalina (AF). Bioensaio em camundongos e pesquisa do DNA do parasito pela reação em cadeia pela polimerase (PCR) do gene 529 pares de bases (pb), repetitivo 300 vezes no genoma do parasito, foi realizado com cérebro, pulmão e músculo ("pool" de diafragma e coração) dos ovinos soropositivos. Das amostras positivas à PCR realizou-se a genotipagem pelas técnicas de multilocus-, nested-PCR e polimorfismo de comprimento dos fragmentos de restrição (RFLP-PCR) em 12 loci (SAG1, 5'SAG2, 3'SAG2, alt-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico, CS3), adicionado de ROP18Del e ROP18UPS, além da confirmação do parasito pelo locus 18S rRNA, diferenciando de Hammondia hammondi, Neospora caninum, Sarcocystis neurona, S. moulei, S. miescheriana, S. hominis, S. capracanis e S. tenella. 66/602 (11,0%) amostras de soro foram positivas para algum teste sorológico, com maior sensibilidade da MAT-AF, utilizado como teste de triagem. Amostras teciduais de 20/66 (30,3%) animais foram positivas ao isolamento, sendo detectados cistos em 12, taquizoítos em quatro e somente sorologia positiva em outras quatro. Detectouse o DNA do parasito em amostras de 22/66 (33,3%) animais. O genótipo completo foi obtido em somente 13/22 (59,1%). Dez genótipos diferentes foram identificados, sendo seis novos genótipos... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Toxoplasma gondii is a parasite with high importance to the animal production and public health, being important opportunistic pathogen in immunocompromised patients. This study was aimed to determine the seroprevalence of toxoplasmosis in ovines in slaughterhouses from State of de São Paulo, and the genotypic variability of the obtained isolates. T. gondii antibodies were researched in 602 serum samples by indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT), with methanol- (AM) and formalin-fixed antigen (AF). Bioassay in mice and the research of DNA by polymerase chain reaction (PCR) of gene 529 base pairs (bp), 300 times repetitive in parasite genome, was assayed with brain, lung and muscle ("pool" of diaphragm and heart) from seropositive ovines. Genotyping of the PCR positive samples was performed by multilocus-, nested-PCR and restriction fragment lenght polymorphism (RFLP-PCR) in 12 loci (SAG1, 5'SAG2, 3'SAG2, alt-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3), added of ROP18Del and ROP18UPS, beyond the confirmation by 18S rRNA locus, to differentiate Hammondia hammondi, Neospora caninum, Sarcocystis neurona, S. moulei, S. miescheriana, S. hominis, S. capracanis and S. tenella. 66/602 (11.0%) serum samples were positive to, at least, one serological test with high sensitivity to MAT-AF, used as screening test. Tissue samples of 20/66 (30.3%) animals were positive to the isolation, being detected cysts in 12, tachyzoites in four and in other four only positive result to the serology. DNA of the parasite was detected in samples of 22/66 (33.3%) animals. The complete genotype was detected in only 13/22 (59.1%). Ten different genotypes were identified, with six new genotypes. Two samples presented type II, isolated from Polwarth (Ideal) breed, bred extensively in Santana do Livramento and Uruguaiana, RS. Two other samples, from... (Complete abstract click electronic access below) / Doutor

This thesis deals with creating files of maps of South Bohemia and the Czech Republic on the economic structure of the population. The chapters describe briefly how to create maps in ArcGIS 9.1. environment and basic elements of cartography. The thesis contains chapters draft cartographic means of expression and basic characteristics and problems counting people, houses and flats 2001. In conclusion, the analytical commentary on the various maps and map layout itself.

Partindo do pressuposto da existência de um paradigma constituído pelo modelo pioneiro de restauração de casas bandeiristas estabelecido pelo IPHAN desde os anos iniciais de sua atividade em São Paulo, o presente trabalho pretende realizar uma análise, em relação àquele paradigma, dos projetos e obras de restauração de cinco casas bandeiristas pertencentes à Prefeitura de São Paulo. Adotando a hipótese da sucessão cronológica de três momentos de continuidade, de diluição e de abandono do paradigma , o presente trabalho busca verificá-la através da análise dos projetos e das obras de restauração realizadas nos exemplares selecionados. Esses projetos e obras foram orientados inicialmente pelo IPHAN Instituto do Patrimônio Histórico e Artístico Nacional e depois, pelo DPH Departamento do Patrimônio Histórico da Prefeitura de São Paulo no período compreendido entre as décadas de 1950 e 2000. / This work analyses the architectural restoration of five bandeirantes houses in the city of São Paulo. Those restoration works, which took place between the years 1950 and 2000, were accomplished by the IPHAN Institute for National Artistic and Historical Heritage and the DPH Historical Heritage Department of the City Government of São Paulo. The work aims to identify and characterize both the conceptual and technical models that guided those restoration works. Assuming that IPHAN has developed a conceptual and technical paradigm in the restoration of this architectonical type, the work investigates the paradigms permanence extent in those five houses and, reversely, its vanishing along the almost fifty years comprising this study.

Esta dissertação busca, inicialmente, investigar formas de mobilidade habitacional, sobretudo, em contextos urbanos. Partindo da hipótese de que a mobilidade é um fenômeno existente e crescente, apresentam-se seus reflexos na arquitetura. Propõe-se à arquitetura, voltada em essência, para formas estáticas, uma reflexão sobre seu interesse e possibilidades na produção de casas móveis. Para contribuir com a arquitetura em face desse paradigma são: investigados na história grupos marcados pela mobilidade; expostos os efeitos possíveis sobre a questão do solo urbano; apresentados os aspectos jurídicos relacionados à habitação sobretudo, a sua mobilidade; mostrados alguns modelos de mobilidade utilizados. Ainda, após pesquisa de campo, expõe-se um caso de transposição de casas de madeira, construídas, em sua maioria, pelos carpinteiros migrantes e emigrantes no Oeste do Paraná, de 1940 a 1970. Essas construções, que outrora sustentaram a maior parte da demanda habitacional da região, são, hoje, viabilizadoras de uma prática local que expressa movimentos contemporâneos de proporções globais. / This study seeks initially for investigating ways of habitational mobility, especially in urban contexts. Starting from the hypothesis that mobility is an existing and increasing phenomenon, they present their reflexes in the architecture. The architecture proposes turned in essence to static forms, a reflection about its interest and possibilities of mobile house production. To contribute with the architecture in view of this paradigm are investigated groups marked by the mobility in the history, exposed the possible effects about the urban soil matter presented the juridical aspects related to habitation especially to its mobility and shown some models of used mobility, yet after field research a wooden house transposition case is exposed, built in its majority by the migrant carpenters and emigrants in Paraná´s West from 1940 to 1970. These constructions that formerly sustained most habitational demand of the regions they are today facilitators of a local practice that expresses contemporary actions of global proportions.