Made available in DSpace on 2014-08-20T14:30:09Z (GMT). No. of bitstreams: 1 Dissertacao_tania_lopez_martinez.pdf: 1972521 bytes, checksum: 41c2bc83c8d268391e9ff0e9b89adf61 (MD5) Previous issue date: 2012-12-21 / The black stain are dental extrinsic pigmentations that has been associated with low caries risk. This study was divided in two parts, the first was a cross-sectional study with schoolchildren between 8 to 12 years old; the aim was determine the prevalence of black stain on the teeth and his relation with demographic and socioeconomics conditions, oral hygiene habits, presence of plaque and dental caries. 1175 children were included from 20 private and public school of Pelotas´s city. Was performed a questionnaire to parents of schoolchildren, interviews and clinical examinations to the students. In the second part was a case control study performed based on the oral survey in schoolchildren with black stain on the teeth and the control are also students that had participated in the survey, using for each case a control. The objectives were evaluated the association between the use or ferrous sulfate in childhood, the ingestion of foods containing iron ant the water source with the presence of black stain on the teeth; and describe the clinical characteristics of pigmentation, evaluated 56 children (28 case and 28 controls). Descriptive analysis and chi-square test to assess association; univariated and multivariated logistic regression to obtain odds ration (OR) with a confidence interval of 95% (IC). The first part concluded that the prevalence of black stain was 5% and an association was found with low family income, in the students who study in public schools and with low frequency of brushing. In the second part was no association between ferrous sulfate, the ingestion of foods containing iron and the water source with black stain on the teeth and was found that the black stains are more prevalent on the anterior region / As manchas dentárias negras (MDN) são pigmentações extrínsecas que têm sido associadas com baixa experiência de cárie. Este estudo foi dividido em duas partes, sendo a primeira foi um estudo transversal de base escolar, realizado na cidade de Pelotas, em 2010 com escolares de 8 a 12 anos de idade, com objetivo de determinar a prevalência das MDN e sua relação com fatores demográficos, socioeconômicos, frequência de escovação, presença de placa e cárie dentária. Foram estudadas 1.157 escolares, alunos de 20 escolas públicas e privadas, foi realizado um questionário aos pais dos escolares, entrevistas e exame clínico bucal aos escolares. A segunda parte foi um estudo caso-controle baseado no levantamento de saúde bucal em escolares, os casos foram 28 escolares portadores de MDN e os controles 28 escolares não portadores, participantes do levantamento, adotando a razão de 1:1. O objetivo foi avaliar a associação entre o uso do sulfato ferroso na infância, o consumo de alimentos que contém ferro e a fonte de água com a presença das MDN e descrever as características clínicas das pigmentações. Foram realizadas análises descritivas, o teste qui-quadrado nas associações e regressão logística uni e multivariada para obter as razões de chances (OR) com um intervalo de confiança de 95% (IC). Da primeira parte conclui-se que a prevalência das MDN foi de 5% e encontrou-se associação com a baixa renda familiar, nos escolares que estudam na rede pública e com a baixa frequência de escovação. No estudo caso-controle não foi encontrada associação entre o sulfato ferroso, o consumo de alimentos contendo ferro e a fonte de agua com as MDN, sendo que as mesmas foram mais prevalentes na região anterior

[pt] Este trabalho estabelece um percurso na obra freudiana e no ensino de J. Lacan em torno do objeto da psicanálise e de sua relação com a arte. Nosso caminho se inicia com S.Freud, no registro da experiência, especialmente no que se refere ao texto o Estranho de 1919, passando pelo ensino de Lacan, notadamente nos seus Seminários sobre a Ética, a Angústia e em seu estudo de 1964 sobre o olhar como objeto a minúsculo. Durante este trajeto, fizemos contato com a obra de artistas, predominantemente visuais, o que nos confirmou a sentença de Freud, reafirmada por Lacan, segundo a qual o artista precede o psicanalista. Esta sentença estabeleceu para nós uma orientação: estudamos o conceito lacaniano de real e a maneira como a arte pode transmitir e ensinar ao psicanalista formas de lidar com isso que escapa e excede à articulação simbólica. Estudamos como certas imagens seriam capazes de circunscrever esse excesso em uma composição que, embora não o acomode completamente, ofereça um lugar possível a ele. Ao final, uma direção clínica distinta nos tomou a atenção e apontou para a clínica das psicoses - para além da estrutura e do objeto. Nessa etapa, o artista pode mais uma vez nos guiar a partir de um paralelo traçado entre a invenção, formulada por Miller e atribuída à psicose, e a construção de arranjos e soluções subjetivas singulares próximas à criação e ao fazer artístico. / [en] This work establishes a path in the Freudian work and in the teaching of J. Lacan around the object of psychoanalysis and its relationship with art. Our route begins with S.Freud, in the scope of the experience, especially with regard to the text the Uncanny of 1919, passing through Lacan s teaching, notably in his Seminars on Ethics, Anguish and in his 1964 study about the gaze as an object (petit a). During this journey, we made contact with the work of artists, predominantly visual, what confirmed us Freud s sentence, reaffirmed by Lacan, according to which the artist precedes the psychoanalyst. This sentence established for us an orientation: we study the Lacanian concept of the real and the way that art can transmit and teach the psychoanalyst ways of dealing with what escapes and exceeds symbolic articulation. We studied how certain images would be able to circumscribe this excess in a composition that, although it does not accommodate it completely, offers a possible place for it. At the end, a different clinical direction took our attention and pointed to the clinic of psychoses - beyond the structure and the object. At this stage, the artist can once again guide us from a parallel drawn between the invention, formulated by Miller and attributed to psychosis, and the construction of unique subjective arrangements and solutions close to creation and artistic making.

Interlocking Cross Laminated Timber (ICLT) wall panels are a new wood construction product similar to Cross Laminated Timber panels. Besides being an innovative structural system, they also utilize beetle killed timber from many of the forests that have been devastated by the Mountain Pine Beetle. Three tests were performed on three ply ICLT panels measuring 8 feet (2.44m) wide, 8 feet (2.44m) tall and 8.5 inches (21.6cm) thick to determine the racking, flexural and axial strengths of the wall panels. After each test was performed the walls were disassembled and investigated for cause of failure. Using the data from the tests as a benchmark, simple analytical models to predict the design capacities of the walls for racking, flexural, and axial strengths were established. The analytical models for racking strength, flexural strength and axial strength predicted reasonably well the measured strength values. Additional testing is necessary to increase the available database, further validate the analytical models developed, better understand the structural performance of ICLT panels, and establish acceptable design methodology for ICLT wall panels.

Le virus de la grippe est un virus à ARN négatif appartenant à la famille des Orthomyxoviridae qui se compose de 7 membres dont les virus influenza A, B, C et D. Le génome viral comprend 7 à 8 particules ribonucléoprotéiques (RNP) au sein desquelles l’ARN viral (ARNv) est recouvert de multiples copies de nucléoprotéines (NP) et est associé à l’ARN polymérase virale via ses extrémités 3’ et 5’. Au cours de ce travail de thèse, nous nous sommes tout d’abord focalisés sur l’étude biochimique de NP A et NP B et avons pu mettre en évidence des comportements différents en ce qui concerne leurs propriétés d’oligomérisation en présence ou en absence d’ARN et en fonction de la concentration en sel. Pour la première fois nous avons pu observer une structure similaire aux RNP mais reconstituée uniquement à partir de NP A et d’un ARN de 12 nucléotides. Nous avons pu formuler l’hypothèse que 12 nucléotides de l’ARN serait fixés à la NP avec une forte affinité tandis que le reste de l’ARN fixerait la NP avec une affinité beaucoup plus faible. En parallèle nous avons résolu la structure cristallographique de la nucléoprotéine de la grippe de type D et réaliser la caractérisation de son interaction avec l’importine-α7 humaine. Enfin nous avons étudié la fixation de l’ARN sur NP D et mis en évidence l’importance de l’extrémité C-terminale dans le processus de fixation à l’ARN. Ces informations ont permis de formuler de nouvelles hypothèses quant au fonctionnement du virus de la grippe et permettre d’inscrire ce projet de thèse dans une dynamique globale de lutte contre ce virus. / Influenza virus is a negative RNA virus belongs to the Orthomyxoviridae family which consists of 7 members including influenza viruses A, B, C and D. The viral genome comprises 7 to 8 ribonucleoprotein particles (RNP) in which the viral RNA (vRNA) is coated with multiple copies of nucleoproteins (NP) and is associated with the viral RNA polymerase by its 3 'and 5' ends. In this thesis, we first focused on the biochemical study of NP A and NP B and we demonstrate that there are different behaviors with regard to their oligomerization properties in the presence or absence of RNA and as a function of the salt concentration. For the first time we were able to observe a structure very similar to RNP which was reconstituted only from NP A and a 12 nucleotide RNA. Thus, we formulate the hypothesis that 12 nucleotides of the RNA would bind NP with a very strong affinity while the rest of the RNA would bind NP with a lower affinity. In parallel, we solved the crystallographic structure of the nucleoprotein of influenza D virus and we characterized its interaction with human importin-α7. Finally, we studied the binding of RNA on NP D and we demonstrated the importance of the C-terminal end in the RNA binding process. This thesis project made it possible to formulate new hypotheses concerning the functioning of the influenza virus and to include this thesis project in a global dynamic of combating the influenza virus.

Made available in DSpace on 2016-04-28T14:59:00Z (GMT). No. of bitstreams: 1 2008 - Debora Alves Gonzaga da Silva1.pdf: 485199 bytes, checksum: 8ad8d9c6be0e3c6fca263c141e2866c3 (MD5) Previous issue date: 2008-09-26 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / In the objective of advancing the epidemiologic study of the bacterial stain of the tomato, the mechanisms of transmission of Xanthomonas vesicatoria from the plant to the seed and from the seed to the plant have been evaluated. The morphology of seeds and of parts of the seedlings or saplings (tegument, radicles, hypocotyl and cotyledon leaves, and definitive leaves) have also been studied by means of electronic scanning microscopy techniques. The transmission of X. vesicatoria from the plant to the seed has been evaluated upon direct and indirect isolation of parts of ripe fruit (flesh, placenta, placental liquid, water from the first washing of the seeds, entire seeds and ground seeds) in a culture of Agar Nutrient (AN). The fruit were obtained in experimental parcels, conducted at the Horticultural sector of UFRRJ, and inoculated with the phytobacteria through different methods (atomization and injection) combined with different regions of inoculation (flower cluster, flesh, and placenta), during the plant s developing phases (flower, unripened fruit, firm ripe and ripe fruit). The transmission of the phytobacteria from the seeds to the seedlings and saplings was assessed by means of isolating in an AN medium parts of the seedling or sapling (root, tegument, radicles, hypocotyl and cotyledon leaves, and definitive leaves), germinated in different substrata ( germitest paper, sand, and commercialized substrate for saplings). The morphology of seeds and parts of the seedlings was characterized through observations in electron microscope scanning by using samples of fresh seeds (extracted from inoculated fruit), of parts of seedlings or saplings (tegument, radicles, hypocotyl and cotyledon leaves, and definitive leaves) and of seeds inoculated by vacuum procedure at 7, 14, and 21 days after sowing. The treatments applied to the unripened fruit (inoculation by atomization, injection in the placenta and injection in the flesh) were more efficient in the process of transmitting the phytobacteria than the treatment applied to the firm ripe and ripe fruit. It was also observed that the X vesicatoria colonizes the tegument and every part of the seedlings and saplings during the process of germination and emergence. The seed tegument was characterized by an entanglement of trichomes, with a base in the shape of ring and cavity, which may serve as sheltering sites for pathogens, including the X vesicatoria. The process of colonization of tomato seeds by X vesicatoria characterizes itself by the formation of biofilms and fibrils. The presence of stomata colonized by X vesicatoria has been observed in radicles, of seven day old seedlings, and in primary roots originated from the radicles of 20 day old saplings. Few cells of X vesicatoria have been observed on the hypocotyl. The presence of endophytic bacteria forming aggregates with characteristics of an aggressive growth identified as Acinetobacter sp. was detected in many samples of seeds. These same bacteria were detected in many tests of germination and isolation, interfering negatively in the development and recuperation of X vesicatoria in the in vitro tests. / Com o objetivo de se avan?ar no estudo epidemiol?gico da mancha-bacteriana do tomateiro, foram avaliados os mecanismos de transmiss?o de Xanthomonas vesicatoria da planta para a semente e da semente para a planta. Foram feitos, ainda, estudos da morfologia da semente e partes da pl?ntula ou muda (tegumento, rad?cula, hipoc?tilo e folhas cotiledonares e definitivas), atrav?s de t?cnicas de microscopia eletr?nica de varredura. A transmiss?o de X. vesicatoria da planta para a semente foi avaliada a partir de isolamentos diretos e indiretos de partes de frutos maduros (mesocarpo, placenta, l?quido placent?rio, ?gua proveniente da primeira lavagem das sementes, sementes inteiras e sementes trituradas) em meio de cultura Nutriente Agar (NA). Os frutos foram obtidos em parcelas experimentais, conduzidas no setor de Horticultura da UFRRJ, e inoculados com a fitobact?ria por diferentes m?todos (atomiza??o e inje??o) combinados com diferentes regi?es de inocula??o (cacho floral, mesocarpo e placenta), durante as fases de desenvolvimento da planta (flor, frutos verdes, frutos de vez e frutos maduros). A transmiss?o da fitobact?ria das sementes para as pl?ntulas e mudas foi avaliada por isolamentos em meio NA a partir de partes da pl?ntula ou muda (raiz, tegumento, hipoc?tilo, folhas cotiledonares e folhas definitivas), germinadas em diferentes substratos (papel germitest, areia e substrato comercial para mudas). A morfologia das sementes e partes das pl?ntulas foi caracterizada por meio de observa??es em microsc?pio eletr?nico de varredura utilizando-se amostras de sementes frescas (extra?das de frutos inoculados), de partes de pl?ntulas ou mudas (raiz, hipoc?tilo, folha cotiledonar e folha definitiva) e de sementes inoculadas pelo procedimento a v?cuo aos 7, 14 e 21 dias ap?s a semeadura. Os tratamentos aplicados aos frutos verdes (inocula??o por atomiza??o, inje??o na placenta e inje??o no mesocarpo) foram mais eficientes no processo de transmiss?o da fitobact?ria que os tratamentos aplicados aos frutos de vez e maduros. Observou-se, ainda, que X. vesicatoria coloniza o tegumento e todas as partes das pl?ntulas e mudas durante o processo de germina??o e emerg?ncia. O tegumento da semente foi caracterizado por um emaranhado de tricomas, com a base em forma de anel e cavidade, que podem servir como s?tios protetores para pat?genos inclusive X. vesicatoria. O processo de coloniza??o das sementes de tomate por X. vesicatoria se caracteriza pela forma??o de biofilmes e fibrilas. Foram observados em rad?culas, de pl?ntulas com sete dias de idade, e em ra?zes prim?rias, oriundas das rad?culas de mudas com 20 dias de idade, a presen?a de est?matos colonizados por X. vesicatoria. Sobre o hipoc?tilo, foram observadas poucas c?lulas de X. vesicatoria. Em v?rias amostras de sementes foi detectada a presen?a de bact?rias endof?ticas, que formavam agregados com caracter?sticas de crescimento agressivo, identificadas como Acinetobacter sp. Esta mesma bact?ria foi detectada em v?rios testes de germina??o e isolamento interferindo negativamente no desenvolvimento e recupera??o de X. vesicatoria nos testes in vitro.

The mechanical behaviour of geomaterials (e.g. soils, rocks and concrete) under plastic deformation is highly complex due to that fact that they are granular materials consisting of discrete non-uniform particles. Failure of geomaterials is often related to localisation of deformation (strain-localisation) with excessive shearing inside the localised zones. The microstructure of the material then dominates the material behaviour in the localised zones. The formation of the localised zone (shear band) during plastic deformation decreases the material strength (softening) significantly and initiates the failure of the material. There are two main approaches to the numerical modelling of localisation of deformation in geomaterials; discrete and continuum. The discrete approach can provide a more realistic material description. However, in the discrete approach, the modelling of all particles is complicated and computationally very expensive for a large number of particles. On the other hand, the continuum approach is more flexible, avoids modelling the interaction of individual particles and is computationally much cheaper. However, classical continuum plasticity models fail to predict the localisation of deformation accurately due to loss of ellipticity of the governing equations, and spurious mesh-dependent results are obtained in the plastic regime. Generalised plasticity models are proposed to overcome the difficulties encountered by classical plasticity models, by relaxing the local assumptions and taking into account the microstructure-related length scale into the models. Among generalised plasticity models, Cosserat (micropolar) and stain-gradient models have shown significant usefulness in modelling localisation of deformation in granular materials in the last few decades. Currently, several elastoplastic models are proposed based on Cosserat and strain-gradient theories in the literature. The individual formulation of the models has been examined almost always in isolation and are paired with specific materials in a mostly arbitrary fashion. Therefore, there is a lack of comparative studies between these models both at the theory level and in their numerical behaviour, which hinders the use of these models in practical applications. This research aims to enable broader adoption of generalised plasticity models in practical applications by providing both the necessary theoretical basis and appropriate numerical tools. A detailed comparison of some Cosserat and strain-gradient plasticity models is provided by highlighting their similarities and differences at the theory level. Two new Cosserat elastoplastic models are proposed based on von Mises and Drucker- Prager type yield function. The finite element formulations of Cosserat and strain-gradient models are presented and compared to better understand their advantages and disadvantages regarding numerical implementation and computational cost. The finite elements and material models are implemented into the finite element program ABAQUS using the user element subroutine (UEL) and an embedded user material subroutine (UMAT) respectively. Cosserat finite elements are implemented with different Cosserat elastoplastic models. The numerical results show how the Cosserat elements behaviour in the plastic regime depends on the models, interpolation of displacement and rotation and the integration scheme. The effect of Cosserat parameters and specific formulations on the numerical results based on the biaxial test is discussed. Two new mixed-type finite elements as well as existing ones (C1, mixed-type and penalty formulation), are implemented with different strain-gradient plasticity models to determine the numerical behaviour of the elements in the plastic regime. A detailed comparison of the numerical results of Cosserat and strain-gradient elastoplastic models is provided considering specific strain-localisation problems. Finally, some example problems are simulated with both the Cosserat and strain-gradient models to identify their applicability.

Magistriniame darbe nustatytos legiruotųjų konstrukcinių plienų ir jų suvirinimo siūlių medžiagų mechaninių ir deformavimo diagramos charakteristikų A1, α, sT priklausomybės būdingame intervale kambario ir aukštesnėje temperatūroje. Nuosekli statistinė analizė parodė, kad duomenys yra atsitiktiniai, nepriklausomi bei pasiskirstę pagal normalųjį dėsnį, o atlikta tyrimo rezultatų koreliacinė ir regresinė analizė, kad tarp daugumos medžiagų grupių deformavimo diagramos charakteristikų ir modifikuoto plastiškumo kriterijaus egzistuoja reikšmingas tiesinis koreliacinis ryšys. Darbe ciklinėms deformavimo diagramos charakteristikoms pagal mechanines savybes nustatyti, buvo apskaičiuoti analitinių priklausomybių koeficientai, bei patikrintas regresijos lygčių adekvatumas. Nustatant deformavimo diagramos charakteristikų reikšmes nuo modifikuoto plastiškumo kriterijaus nustatytame būdingame intervale gaunami tikslesni rezultatai, nes daugumai skaičiuotų ir eksperimentinių charakteristikų rezultatų sklaida intervaluose yra mažesnė lyginant su bendrų plienų ir jų suvirinimo siūlių medžiagų grupių rezultatų sklaida. Darbe pasiūlyti tiesinės priklausomybės koeficientai nuo modifikuoto plastiškumo kriterijaus, gali būti pritaikyti nustatant preliminarų inžinerijos šakose (energetikoje, naftoje) dirbančių mašinų ilgalaikiškumą, atsižvelgiant į jų eksploatacijos aplinką, darbo temperatūrą, naudojamas medžiagas. Magistrinio darbo tema buvo skaitoma ir aptarta 2013 m. gegužės 15 d. Šiaulių... [toliau žr. visą tekstą] / In this master thesis alloy structural steels and their weld joint materials cyclic stress–stain curves parameters A1, α, sT dependences on mechanical characteristic in the typical range at room and elevated temperatures are determined. Consistent statistical analysis of experimental results shown, that data is accidental, independence and normal distributed, also correlation - regression analysis shown, that almost for all groups of the materials exists a significant linear correlation between cyclic stress–stain curves parameters and modified plasticity criterion. In this final work, for parameters A1, α, sT from mechanical properties to determine, was calculated analytical dependence coefficients, also regression equations adequacy was verified. Determining value of deformation parameters from modified plasticity criterion in typical range, is better way, because the results is more accurate this is because for almost all computed and experimental characteristics results dispersion in ranges are lower comparing with total groups of alloy steels and their weld joint materials results dispersion. In this master thesis are suggested linear regression coefficients from modified plasticity criterion, which can be used for preliminary evaluation of lifetime for engineering industries machines considering of their operational environment, working temperature and what materials are used for machine‘s construction. Master's thesis has been read and discussed in May 15, 2013 at... [to full text]

This study quantifies discolouration on the surface of Pinus radiata boards during kiln drying, particularly kiln brown stain (KBS), and models it as a function of chemical compounds present in the wood closest to the surface. The discolouration was investigated with two experimental factors: drying time, which consisted in drying at 70/120 ℃ for 0, 8, 16 and 24 hours; and leaching, done at three levels, noleaching, mild and severe, to reduce the soluble compounds present in wood suspected of developing coloured compounds. The colour change was quantified using a reflectance photometer (colour system CIE Yxy, brightness) and by the analysis of digital photographs (colour system CIE Lab). The chemical analysis of the wood closest to the surface of the boards determined fructose, glucose, sucrose (HPLC), total sugar (sum of fructose, glucose and sucrose), total nitrogen (combustion gas analysis), and phenols discriminated by molecular weight (Folin-Ciocalteu method). In the cause-effect analysis, colour was the dependent variable, and drying time and the determinations of chemical compounds were independent variables. After statistical analysis (ANOVA and MANOVA) the dependent variables to be included in the models were luminance factor (Y), brightness (R457 and the blue-to-yellow scale of CIE Lab (b); and the independent variables were drying time, nitrogen, total sugar, and high-molecular-weight phenols. Linear (multivariate regression) and non-linear models (Neural Networks) showed that discolouration during kiln drying was best predicted when the luminance factor (Y) was used to quantify colour change as a function of the content of nitrogen-containing compounds and drying time. Furthermore, the data were fitted into an empirical model based on simple reaction kinetics that considered the rate of discolouration as a function of nitrogen concentration. The results suggest that nitrogen could act as a limiting reactant in Maillard-type reactions that produce colour during kiln drying.

Les biopuces connaissent un grand essor depuis quelques années avec des applicationspossibles pour l'ADN, les protéines et les oligosaccharides. Une puce à oligosaccharidesprésente des difficultés par rapport à une puce à ADN notamment par les contraintes entempérature et il existe moins de travaux dans ce domaine. Ce travail est donc consacré àl'étude d'une puce à oligosaccharide, par synthèse supportée et par masquage avec un film depolymère. Le procédé de fabrications est particulièrement détaillé.Nous étudions tout d'abord expérimentalement la formation d'un film de polymère obtenu parévaporation d'une goutte de solution polymérique sur une surface structurée chimiquement(zone de mouillabilité différente) en suivant son évolution transitoire. Nous montrons que cetype de surface hétérogène est particulièrement adapté pour la fabrication de biopuces.D'autre part, nous réalisons un modèle numérique de l'évaporation d'une goutte de solutionpolymérique sur une surface chauffée à partir de la méthode de la lubrification et d'un modèlede " hauteur de résine ". Les résultats expérimentaux et de simulation numérique sontcomparés et montrent un bon accord qualitatif sur la forme des films de polymères résultantde l'évaporation.Dans ce travail, la synthèse supportée de biopuces à oligosaccharide est menée à bien enutilisant des polymères et des surfaces judicieusement choisies. En particulier, la technique demasquage par film de polymère se révèle être très bien adaptée pour protéger les oligomères àla fois à hautes et à très basses températures.

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