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The Global ETD Search service is a free service for researchers
to find electronic theses and dissertations. This service is
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Networked Digital Library of Theses and Dissertations.
Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
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Showing 31 to 40 of 46 (0.007 seconds)| 31 |
Growth kinetics of a Helianthus annuus and a Salvia fruticosa suspension cell line: Shake flask cultivations with online monitoring systemGeipel, Katja, Socher, Maria Lisa, Haas, Christiane, Bley, Thomas, Steingroewer, Juliane 15 November 2016 (has links) (PDF)
Plants produce a variety of secondary metabolites, e.g. to defend themselves against herbivores or to attract pollinating insects. Plant cell biotechnology offers excellent opportunities in order to use such secondary plant metabolites to produce goods with consistent quality and quantity throughout the year, and therefore to act independently from biotic and abiotic environmental factors.
This article presents results of an extensive study of plant cell in vitro cultivation in a modern shake flask system with non-invasive online respiration activity monitoring unit. Comprehensive screening experiments confirm the successful transfer of a model culture (sunflower suspension) into the shake flask monitoring device and the suitability of this respiration activity monitoring unit as qualified tool for screening of plant in vitro cultures (sunflower and sage suspension).
The authors demonstrate deviations between online and offline data due to varying water evaporation from different culture flask types. The influence of evaporation on growth-specific parameters thereby rises with increasing cultivation time. Furthermore, possibilities to minimize the impact of evaporation, either by adjusting the inlet air moisture or by measuring the evaporation in combination with an appropriate correction of the measured growth values, are shown.
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Development and analysis of a mechanical gripper in a GoFa ABB robotEscobar Hidalgo, Cristina January 2024 (has links)
This study focuses on the design and analysis of a specialised mechanical gripper for an assembly process of batteries, with particular emphasis on the study of the associated forces and deformations. The project commences with comprehensive market research to identify existing solutions. This is followed by the definition of requirements and an iterative design process utilising computer-aided design (CAD). Subsequently, a comprehensive force and deformation analysis is conducted using the finite element method (FEM) in Abaqus CAE. The results demonstrate that the designed gripper can withstand the applied loads with minimal deformation, indicating that it possesses adequate structural stiffness. The utility of finite element method (FEM) analysis in evaluating the viability of the proposed design is demonstrated. According to the design and analysis in this study, it manages to propose and developed a new kind of gripper with a higher grip range than those available on the market. These findings contribute to a deeper understanding of the suitability of the gripper design in relation to the expected loads and highlight the importance of the design methodology employed.
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Multipose Binding in Molecular DockingAtkovska, Kalina, Samsonov, Sergey A., Paszkowski-Rogacz, Maciej, Pisabarro, M. Teresa 09 July 2014 (has links) (PDF)
Molecular docking has been extensively applied in virtual screening of small molecule libraries for lead identification and optimization. A necessary prerequisite for successful differentiation between active and non-active ligands is the accurate prediction of their binding affinities in the complex by use of docking scoring functions. However, many studies have shown rather poor correlations between docking scores and experimental binding affinities. Our work aimed to improve this correlation by implementing a multipose binding concept in the docking scoring scheme. Multipose binding, i.e., the property of certain protein-ligand complexes to exhibit different ligand binding modes, has been shown to occur in nature for a variety of molecules. We conducted a high-throughput docking study and implemented multipose binding in the scoring procedure by considering multiple docking solutions in binding affinity prediction. In general, improvement of the agreement between docking scores and experimental data was observed, and this was most pronounced in complexes with large and flexible ligands and high binding affinities. Further developments of the selection criteria for docking solutions for each individual complex are still necessary for a general utilization of the multipose binding concept for accurate binding affinity prediction by molecular docking.
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Fear Processing in Dental Phobia during Crossmodal Symptom Provocation: An fMRI StudyHilbert, Kevin, Evens, Ricarda, Maslowski, Nina Isabel, Wittchen, Hans-Ulrich, Lüken, Ulrike 09 July 2014 (has links) (PDF)
While previous studies successfully identified the core neural substrates of the animal subtype of specific phobia, only few and inconsistent research is available for dental phobia. These findings might partly relate to the fact that, typically, visual stimuli were employed. The current study aimed to investigate the influence of stimulus modality on neural fear processing in dental phobia. Thirteen dental phobics (DP) and thirteen healthy controls (HC) attended a block-design functional magnetic resonance imaging (fMRI) symptom provocation paradigm encompassing both visual and auditory stimuli. Drill sounds and matched neutral sinus tones served as auditory stimuli and dentist scenes and matched neutral videos as visual stimuli. Group comparisons showed increased activation in the insula, anterior cingulate cortex, orbitofrontal cortex, and thalamus in DP compared to HC during auditory but not visual stimulation. On the contrary, no differential autonomic reactions were observed in DP. Present results are largely comparable to brain areas identified in animal phobia, but also point towards a potential downregulation of autonomic outflow by neural fear circuits in this disorder. Findings enlarge our knowledge about neural correlates of dental phobia and may help to understand the neural underpinnings of the clinical and physiological characteristics of the disorder.
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Forest Management Approaches for Coping with the Uncertainty of Climate Change: Trade-Offs in Service Provisioning and AdaptabilityWagner, Sven, Nocentini, Susanna, Huth, Franka, Hoogstra-Klein, Marjanke 01 August 2014 (has links) (PDF)
The issue of rapid change in environmental conditions under which ecosystem processes and human interventions will take place in the future is relatively new to forestry, whereas the provision of ecosystem services, e.g., timber or fresh water, is at the very heart of the original concept of forest management. Forest managers have developed ambitious deterministic approaches to provide the services demanded, and thus the use of deterministic approaches for adapting to climate change seem to be a logical continuation. However, as uncertainty about the intensity of climate change is high, forest managers need to answer this uncertainty conceptually. One may envision an indeterministic approach to cope with this uncertainty; but how the services will be provided in such a concept remains unclear. This article aims to explore the fundamental aspects of both deterministic and indeterministic approaches used in forestry to cope with climate change, and thereby point out trade-offs in service provisioning and adaptability. A forest owner needs to be able to anticipate these trade-offs in order to make decisions towards sustainable forest management under climate change.
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Fluidic microchemomechanical integrated circuits processing chemical informationGreiner, Rinaldo, Allerdissen, Merle, Voigt, Andreas, Richter, Andreas 08 April 2014 (has links) (PDF)
Lab-on-a-chip (LOC) technology has blossomed into a major new technology fundamentally influencing the sciences of life and nature. From a systemic point of view however, microfluidics is still in its infancy. Here, we present the concept of a microfluidic central processing unit (CPU) which shows remarkable similarities to early electronic Von Neumann microprocessors. It combines both control and execution units and, moreover, the complete power supply on a single chip and introduces the decision-making ability regarding chemical information into fluidic integrated circuits (ICs). As a consequence of this system concept, the ICs process chemical information completely in a self-controlled manner and energetically self-sustaining. The ICs are fabricated by layer-by-layer deposition of several overlapping layers based on different intrinsically active polymers. As examples we present two microchips carrying out long-term monitoring of critical parameters by around-the-clock sampling. / Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
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Adansonia digitata and Adansonia gregorii fruit shells serve as a protection against high temperatures experienced during wildfiresKempe, Andreas, Neinhuis, Christoph, Lautenschläger, Thea 09 June 2018 (has links) (PDF)
The thick and woody shell of the fruit of Adansonia species cannot be explained solely by adaptation to zoochory or hydrochory. Since the trunks of Adansonia possess a thick and fire-resistant bark and wildfires occur regularly in its habitat (savannah), we examined with the African Adanonia digitata and the Australian Adansonia gregorii whether the fruit offers protection against high heat typically experienced in wildfires. Heat-resistance tests were conducted by applying a simple heat test based on known temperature and temperature residence times occurring in savannah fires and complemented by tests to reveal the impact of heat on germination since long-term seed dormancy is known for Adansonia. Germination tests with acid treated and heat treated seeds were performed to establish if heat also increased germination rate as effectively as acid treatments have been found to do. Heat was found to increase germination rate, but not as effectively as treatment with acid, therefore fruits exposed to high temperatures experienced in wildfires may have a better chance of germination than fruits that were not exposed to wildfires. The ability of the investigated fruits to protect seeds from high temperatures suggests that wildfires may have played a role in the evolution of the hard-shell structure typically found in Adansonia.
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Enzymatic Preparation of 2,5-Furandicarboxylic Acid (FDCA)—A Substitute of Terephthalic Acid—By the Joined Action of Three Fungal EnzymesKarich, Alexander, Kleeberg, Sebastian B., Ullrich, René, Hofrichter, Martin 25 April 2018 (has links) (PDF)
Enzymatic oxidation of 5-hydroxymethylfurfural (HMF) and its oxidized derivatives was studied using three fungal enzymes: wild-type aryl alcohol oxidase (AAO) from three fungal species, wild-type peroxygenase from Agrocybe aegerita (AaeUPO), and recombinant galactose oxidase (GAO). The effect of pH on different reaction steps was evaluated and apparent kinetic data (Michaelis-Menten constants, turnover numbers, specific constants) were calculated for different enzyme-substrate ratios and enzyme combinations. Finally, the target product, 2,5-furandicarboxylic acid (FDCA), was prepared in a multi-enzyme cascade reaction combining three fungal oxidoreductases at micro-scale. Furthermore, an oxidase-like reaction is proposed for heme-containing peroxidases, such as UPO, horseradish peroxidase, or catalase, causing the conversion of 5-formyl-2-furancarboxylic acid into FDCA in the absence of exogenous hydrogen peroxide.
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CellTrans: An R Package to Quantify Stochastic Cell State TransitionsBuder, Thomas, Deutsch, Andreas, Seifert, Michael, Voss-Böhme, Anja 15 November 2017 (has links) (PDF)
Many normal and cancerous cell lines exhibit a stable composition of cells in distinct states which can, e.g., be defined on the basis of cell surface markers. There is evidence that such an equilibrium is associated with stochastic transitions between distinct states. Quantifying these transitions has the potential to better understand cell lineage compositions. We introduce CellTrans, an R package to quantify stochastic cell state transitions from cell state proportion data from fluorescence-activated cell sorting and flow cytometry experiments. The R package is based on a mathematical model in which cell state alterations occur due to stochastic transitions between distinct cell states whose rates only depend on the current state of a cell. CellTrans is an automated tool for estimating the underlying transition probabilities from appropriately prepared data. We point out potential analytical challenges in the quantification of these cell transitions and explain how CellTrans handles them. The applicability of CellTrans is demonstrated on publicly available data on the evolution of cell state compositions in cancer cell lines. We show that CellTrans can be used to (1) infer the transition probabilities between different cell states, (2) predict cell line compositions at a certain time, (3) predict equilibrium cell state compositions, and (4) estimate the time needed to reach this equilibrium. We provide an implementation of CellTrans in R, freely available via GitHub (https://github.com/tbuder/CellTrans).
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Evolution of Epiphytism and Fruit Traits Act Unevenly on the Diversification of the Species-Rich Genus Peperomia (Piperaceae)Frenzke, Lena, Goetghebeur, Paul, Neinhuis, Christoph, Samain, Marie-Stephanie, Wanke, Stefan 19 January 2017 (has links) (PDF)
The species-rich genus Peperomia (Black Pepper relatives) is the only genus among early diverging angiosperms where epiphytism evolved. The majority of fruits of Peperomia release sticky secretions or exhibit hook-shaped appendages indicative of epizoochorous dispersal, which is in contrast to other flowering plants, where epiphytes are generally characterized by fruit morphological adaptations for anemochory or endozoochory. We investigate fruit characters using Cryo-SEM. Comparative phylogenetic analyses are applied for the first time to include life form and fruit character information to study diversification in Peperomia. Likelihood ratio tests uncover correlated character evolution. We demonstrate that diversification within Peperomia is not homogenous across its phylogeny, and that net diversification rates increase by twofold within the most species-rich subgenus. In contrast to former land plant studies that provide general evidence for increased diversification in epiphytic lineages, we demonstrate that the evolution of epiphytism within Peperomia predates the diversification shift. An epiphytic-dependent diversification is only observed for the background phylogeny. An elevated frequency of life form transitions between epiphytes and terrestrials and thus evolutionary flexibility of life forms is uncovered to coincide with the diversification shift. The evolution of fruits showing dispersal related structures is key to diversification in the foreground region of the phylogeny and postdates the evolution of epiphytism. We conclude that the success of Peperomia, measured in species numbers, is likely the result of enhanced vertical and horizontal dispersal ability and life form flexibility but not the evolution of epiphytism itself.
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