Anthropogenic sound has profoundly changed the acoustic environment of aquatic habitats, with growing evidence that even a short exposure to man-made sound sources can negatively affect marine organisms. Marine invertebrates have received little attention regarding their responses to anthropogenic sound, despite their pivotal role in marine ecosystems, and preliminary evidence of their sensitivity. In this thesis, I critically review the methods used in studies investigating the effects of anthropogenic noise on marine invertebrates. I identify methodological trends that have developed along the timeline of this topic, and use this information to suggest three research strategies to further the development of research in this field. From this review, current knowledge gaps are identified, and two main routes to address them are taken in this thesis. Firstly, to address the shortage of particle motion data in anthropogenic sound literature, two new low-cost and easily accessible particle motion sensors were developed and tested, one of them at 0.1% of the cost of currently commercially available models. These sensors will allow the measurement and reproduction of the sound fields experienced by marine invertebrates in bioacoustic research, even when research budgets are tight. Particle motion is the main sound component detected by invertebrates, yet neglected in many aquatic bioacoustical studies. Secondly, to expand on the comparably small quantity of research investigating the effects of anthropogenic sound on marine invertebrates, a series of controlled laboratory experiments were conducted. Two commercially and ecologically important model species were chosen, the blue mussel Mytilus edulis, representing sessile benthic invertebrates, and the European lobster Homarus gammarus, representing mobile benthic invertebrates. Experiments on M. edulis were conducted taking a mechanistic, integrative approach to investigate the effects of sound on multiple levels of biological organisation, including biochemistry, physiology, and behaviour. The ultimate aim was to understand the underlying drivers behind, and interactions between, responses. Comet assay analysis of haemocytes and gill cells demonstrated a significant six-fold higher single strand breakage in the DNA of cells of mussels exposed to ship-noise playback, compared to those kept under ambient conditions. Superoxide dismutase analysis did not identify an excess of superoxide ions, and glutathione, and glutathione peroxidase assays showed no increase in either glutathione or glutathione peroxidase. TBAR assays however revealed 68% more thiobarbituric acid reactive substances, indicating lipid peroxidation in the gill epithelia of noise exposed specimens. Algal clearance rates and oxygen-consumption rates of noise-exposed mussels were significantly lower (84% reduction and 12% reduction respectively), than those of control animals, while valve gape was significantly (60%) wider. This seemingly converse reaction indicates a shock response in mussels with the onset of noise exposure. Finally, at the genetic level, heat shock protein 70 expression was investigated, but no change was identified during noise exposure. Investigation into the noise induced behavioural responses of H. gammarus suggests seasonal differences in behaviour, using movement as a metric, in response to anthropogenic noise playbacks. During both summer and winter exposures, lobsters spent most time away from the highest noise area (98% of the observed time in summer and 78% in winter). The observed seasonal differences in the time spent in the highest noise area (2% in summer and 22% in winter) could be linked to the lobsters' requirement for shelter during winter. This requirement seems to have had a stronger influence over the animals' behaviour than any desire to avoid high noise levels. The information generated in this thesis can be used by researchers working in the field of marine sound to develop well rounded studies exploring the effects of sound on not only marine invertebrates but other faunal groups as well. The construction details provided to produce low cost particle motion sensors, will allow bioacoustic researchers to easily include particle motion measurements in all future studies investigating the effects of sound on fish and invertebrates. The results of the conducted mussel and lobster experiments evidence how multiple aspects of invertebrate biology can be affected by noise. The observed impacts on both sessile and mobile life forms highlight that the effects of noise cannot be fully understood before a wide range of species with different biological and ecological traits have been studied. The integrative approach to noise research used here can serve as a model for other species, and the results pooled to inform governments and industry of the effects of anthropogenic noise in the marine environment.

The purpose of the present study was to examine the effects of positive and negative symptoms, and duration of illness on emotion processes / ability to understand emotion related cues, experience of emotion, and expression of emotion. A total of 46 schizophrenia patients from Ankara Oncology Hospital Psychiatry Clinic / 23 of them were diagnosed with schizophrenia for at least 10 years, and 23 of them were diagnosed for less than 10 years, participated in the study. Besides, a total of 23 non-clinical subjects / which were similar to the clinical group in terms of educational information, participated to the study. A video-clip which consists of scenes that elicited four types of emotions (fear, disgust, sadness, happiness) was presented to the participants. Understanding of emotion related cues and the experienced emotion were rated through self-report. Facial expressions of the participants were rated through their recorded faces by three psychologists, who were specifically trained for emotional facial expression rating. Symptomatology was assessed by Positive and Negative Symptom Scale (PANSS), and Calgary Depression Scale for schizophrenia patients. Presence of any psychopathology of healthy group was measured through Brief Symptom Inventory. Multivariate Analysis of Variance (MANOVA) revealed that chronic patients presented more understanding of context impairments than acute patients and non-clinical group. In addition to these findings, positive symptoms assessed by PANSS were found to be positively correlated with the understanding context impairment. Therefore, It was suggested that positive symptoms, such as hallucinations and delusions interfere with understanding context task. The results were discussed in the light of the literature and clinical and research implications of the study presented. Finally, necessity of using neurological, biological and cognitive assessment methods for further studies was suggested in order to understand deficits in emotional processes.

The purpose of the current study was to examine the path of perceived parenting styles, emotion recognition, emotion regulation, and psychological well-being in terms of depression, obsessive-compulsive disorder and social anxiety symptoms consequently. For the purpose of this study 530 adults (402 female, 128 male) between the ages of 18 and 36 (M = 22.09, SD = 2.78) participated in the current study. The data was collected by a questionnaire battery including a Demographic Category Sheet, Short-EMBU (Egna Minnen Betraffande Uppfostran- My Memories of Upbringing), &ldquo / Reading the Mind in the Eyes&rdquo / Test (Revised), Emotion Regulation Questionnaire, Emotion Regulation Processes, Beck Depression Inventory, Liebowitz Social Anxiety Scale, Maudsley Obsessive Compulsive Inventory, White Bear Suppression Inventory, Thought-Action Fusion Scale, and Emotional Approach Coping Scale. The psychometric properties of Emotion Regulation Questionnaire and Emotion Regulation Processes were investigated and found to have good validity and reliability characteristics. The three sets of hierarchical multiple regression analyses were conducted to reveal the significant associates of psychological well-being. As expected, the results of the current study revealed that perceived parenting styles, different emotion regulation strategies and processes had associated with psychological well-being in terms of depression, obsessivecompulsive disorder and social anxiety symptoms. The findings, and their implications with suggestions for future research and practice, were discussed in the light of relevant literature.

El presente trabajo de investigación se enfoca en dar un panorama amplio sobre los Principios de responsabilidad corporativa aplicada a un modelo societario regulado en la legislación comparada que sirve como clave para moldear las necesidades del empresario peruano. Por lo que en el primer capítulo abordaremos la gestión de la responsabilidad social corporativa, así como los métodos de certificación y la regulación en nuestra legislación. En el segundo capítulo, analizaremos la estructura y naturaleza jurídica de la Sociedad de Bienestar e Interés Colectivo (Soc. BIC) y su avance en la regulación comparada, el cual permite incentivar al sector privado hacia un cambio necesario a fin de contribuir de forma inclusiva los tres sectores del mercado (social, económico y ambiental) Por último en el tercer capítulo afianzaremos todo lo desarrollado en la investigación bajo una nueva visión empresarial, la cual se propone adoptar a la Soc. BIC en nuestro ordenamiento, cuyos nuevos métodos en el proceso de producción de las sociedades mercantiles lo hacen factible, así mismo permitirá una tacita autorregulación que dinamizara el mercado peruano y ofrecerá beneficios a quienes se adhieran a las nuevas exigencias de políticas de responsabilidad social corporativa. Por ello se ofrecerá el perfil de la regulación de la Sociedad BIC.

La saline de Sfax est un milieu hypersalin localisé dans la zone centrale de la côte est de Tunisie. Dans ce travail, nous avons isolé, identifié, et caractérisé des souches halophiles produisant des peptides antimicrobiens (halocines), afin de mieux comprendre leur rôle dans les interactions microbiennes au sein des milieux hypersalins. Deux étangs salins (TS18, 390 g.L-1 NaCl et M1, 200 g.L-1 NaCl) ont été choisis pour l’échantillonnage. Trente-cinq souches de procaryotes halophiles ont été isolées et caractérisées, dont 11 ont présenté une activité antimicrobienne. Parmi ces souches, 3 produisent des substances antimicrobiennes de nature protéique. A l’aide de PCR et RT-PCR nous avons montré que les souches Halobacterium salinarum ETD5 et ETD8 exprimaient le gène de l’halocine S8, un peptide de 3,6 kDa préalablement purifié d’une souche S8a non identifiée. Le peptide a été purifié à partir de cultures de la souche Hbt. salinarum ETD5. Après purification bioguidée, les fractions actives révèlent deux bandes de 8 et 14 kDa présentant une activité antimicrobienne. L’analyse par séquençage Nterminal et spectrométrie de masse a permis d’identifier ces deux halocines. La bande de 8 kDa correspond à une halocine S8 de 81 acides aminés qui subirait une maturation post-traductionnelle protéolytique différente de celle initialement décrite dans la littérature. Le clonage et le séquençage du gène codant le précurseur de l’halocine S8 démontrent que la séquence est identique chez les deux souches ETD5 et S8a. La bande de 14 kDa correspond à une nouvelle halocine, l’halocine S14. L’halocine S14 correspond à une forme tronquée en partie N-terminale de la Mn-superoxyde dismutase (SOD) d’Hbt. salinarum. Il pourrait s’agir d’évolution divergente d’un gène codant deux protéines distinctes, ou d’une maturation différente de la SOD. Ce travail permettra de mieux connaître les molécules intervenant dans les interactions microbiennes dans les milieux hypersalins, des milieux extrêmes susceptibles de révéler des structures et des modes d’action originaux. / The solar saltern of Sfax is a hypersaline located in the central area of the eastern coast of Tunisia. In this study, we isolated, identified, and characterized halophilic strains producing antimicrobial peptides (halocins), aiming to understand their role in microbial interactions in hypersaline environments. Two ponds (TS18, 390 g.L-1 NaCl and M1, 200 g.L-1 NaCl) were selected for sampling. Thirty-five halophilic strains have been isolated and characterized, among which 11 displayed antimicrobial activity. Three of them produced antimicrobial substances of proteinaceous nature. Using PCR and RT-PCR, we have demonstrated that Halobacterium salinarum ETD5 and ETD8 express the gene encoding halocin S8, a 3.6 kDa peptide previously isolated from a strain S8 unidentified. The peptide was purified from cultures of strain Hbt. salinarum ETD5. Following bioguided purification, the active fractions revealed two protein bands of 8 and 14 kDa exhibiting antimicrobial activity. N-terminal sequencing and mass spectrometry analyses allowed identification of these two halocins. The 8 kDa band corresponds to halocin S8, undergoing a different proteolytic post-translational processing from that originallydescribed. Cloning and sequencing of the gene encoding the precursor of halocin S8 showed that the sequence is identical for both strains ETD5 and S8a. The 14 kDa band is a new halocin termed halocin S14. Halocin S14 corresponds to an N-terminally truncated portion of the archaeal Mnsuperoxide dismutase (SOD). This could result from divergent evolution of a gene encoding two distinct proteins, or a different post-translational processing of SOD. Our study helps to better understand which molecules are involved in microbial interactions within hypersaline environments and and how they contribute to the competitions in such extreme environments, which are susceptible to give rise to original structures and modes of action.

Le droit international privé de l’Union européenne se réapproprie le problème de la qualification. Non content d’en altérer fondamentalement la configuration, il en réoriente laborieusement la résolution. Or, la qualité du système tout entier, sa stabilité à un instant t, de même que sa capacité à se développer, à s’ouvrir et à se déployer sur le monde extérieur, sont placées dans l’étroite dépendance de l’efficacité de la qualification ; efficacité qui se mesure à l’aune de l’analyse méthodologique de l’opération, comme de la cohérence des résultats auxquels elle conduit. Et si à ce titre le système déçoit aujourd’hui, il est néanmoins tout aussi possible que souhaitable de le refonder rationnellement. Qui peut le plus peut le moins… Exemple sera donc pris de la matière contractuelle, soumise à la règle la plus complexe qui soit, la plus sujette à polémiques aussi, afin d’ouvrir la voie à davantage de cohérence.La reconfiguration du problème de qualification, ayant sa source dans la rupture imposée par le droit de l’Union entre la législation matérielle et la législation conflictuelle, en accentue naturellement la complexité. Curieusement, la construction jurisprudentielle multiplie sans raison les difficultés, imposant deux nouvelles ruptures, entre l’interprétation de la catégorie érigée au fondement de la compétence et l’interprétation du facteur de rattachement d’une part, entre l’opération de qualification et l’opération de coordination des compétences concurrentes d’autre part. La refondation du système de qualification suppose ainsi la combinaison harmonieuse de ce que la jurisprudence a dispersé, qualification, rattachement et coordination concourant conjointement à la détermination de la compétence spéciale, la cohérence des résultats de la qualification déterminant le choix des orientations méthodologiques de l’opération. Dans cette perspective simplificatrice, parce que la complexité des réponses juridiques ne se conçoit que lorsqu’elle reflète adéquatement et efficacement la complexité des questions, il apparaît naturel de confier à l’opération de qualification les moyens de prévenir les difficultés que ne manque pas de susciter sa pratique purement analytique, cause naturelle d’un dépeçage des situations entraînant à son tour une dispersion excessive du contentieux. Appuyé au contraire sur une opération de qualification à visée préventive, et recourant à des catégories plus synthétiques aux contours flexibles, le système de détermination de la compétence spéciale en matière contractuelle gagnerait en simplicité, en efficacité, en adaptabilité, et il pourrait peut-être être envisagé, enfin, de donner une dimension mondiale à ce qui fonctionnerait à l’échelle régionale. / Formally disassociating the sources of material and conflict legislation, European Private International law alters the classical problem of characterisation, resolution of which cannot be borrowed from BARTIN or RABEL theories any longer. Yet, the quality of the whole system, its stability at a given moment, as well as its capacity to develop and to deploy on the outside world, are placed in the narrow dependence of the efficiency of the characterisation process. Analysing this efficiency calls for an examination of the theoretical and methodological implications, as well as of the consistency of the achieved results. On both grounds, the current system of characterisation is undoubtedly disappointing, and should be reconsidered rationally. In this perspective, attention will be focused on “matters relating to contract”, submitted to the more complex and criticized rule of the Brussels I regulation. The importation of a conflict of laws issue within a conflict of jurisdiction reasoning (De Bloos/Tessili) constitutes a terrible factor of complexity, interpretation of the conflict category belonging to the European legal order while the interpretation of the connecting factor is abandoned to the national legal order. As a consequence, the reasons behind the choice of a specific connecting factor simply cannot impact the characterisation process, as the exclusion of all claims formed by third parties from the scope of article 5-1° illustrates (Handte). Moreover, complexity and heterogeneity of situations seem to radically oppose the exercise of adjudicatory authority by a unique jurisdiction other than that of the defendant’s domicile. In such a context, conflicts of litigations are more than frequent and are not always dealt with in a consistent manner. Gubisch, for instance, coerces the very thing Shenavai and Leathertex prohibit, i.e. the exercise of adjudicatory authority by the first judge seized, be it the judge of a secondary obligation. Kalfelis drastically opposes consolidation of parallel proceedings in the event a litigation implies claims founded on different grounds. Observation can thus be made that the Brussels I system currently separates three intellectual operations; characterisation, location of the connecting factor, and coordination of concurring jurisdictions are insulated from each other. Yet, those three operations not only chronologically follow one another, but also functionally pursue the same objective, and characterisation could, and should be provided with the means to anticipate the following difficulties. In this perspective, it is suggested that the dispersive consequences of every conceivable characterisation should constitute the very cause of the definitive and centralising characterisation. In other words, the results’ consistency, as well as the cohesion of the heterogeneous components of the claim should dictate methodological choices. It appears, in turn, that departing from the dogmatic attachment to actor sequitur and prior tempore would enable a well functioning regional system to deploy rationally on worldwide scale.

Sphaerodoridae is a group of benthic marine worms (Annelida) characterized by the presence of spherical tubercles covering their whole surface. They are commonly considered as belonging to Phyllodocida although sistergroup relationships are still far from being understood. Primary homology assessments of their morphological features are lacking, hindering the appraisal of evolutionary relationships between taxa. Therefore, our detailed morphological investigation focuses on different Sphaerodoridae as well as on other members of Phyllodocida using an integrative approach combining scanning electron microscopy (SEM) as well as immunohistochemistry with standard neuronal (anti-5-HT) and muscular (phalloidin-rhodamine) markers and subsequent CLSM analysis of whole mounts and sections. Furthermore, we provide histological (HES) and light microscopical data to shed light on the structures and hypothetical function of sphaerodorid key morphological features. We provide fundamental details into the sphaerodorid morphology supporting a Phyllodocida ancestry of these enigmatic worms. However, the muscular arrangement and the presence of an axial muscular pharynx is similar to conditions observed in other members of the Errantia too. Furthermore, nervous system and muscle staining as well as SEM and histological observations of different types of tubercles indicate a homology of the so called microtubercles, present in the long-bodied sphaerodorids, to the dorsal cirri of other Errantia. The macrotubercles seem to represent a sphaerodorid autapomorphy based on our investigations. Therefore, our results allow comparisons concerning morphological patterns between Sphaerodoridae and other Phyllodocida and constitute a starting point for further comparative investigations to reveal the evolution of the remarkable Sphaerodoridae.

Sphaerodoridae is a group of benthic marine worms (Annelida) characterized by the presence of spherical tubercles covering their whole surface. They are commonly considered as belonging to Phyllodocida although sistergroup relationships are still far from being understood. Primary homology assessments of their morphological features are lacking, hindering the appraisal of evolutionary relationships between taxa. Therefore, our detailed morphological investigation focuses on different Sphaerodoridae as well as on other members of Phyllodocida using an integrative approach combining scanning electron microscopy (SEM) as well as immunohistochemistry with standard neuronal (anti-5-HT) and muscular (phalloidin-rhodamine) markers and subsequent CLSM analysis of whole mounts and sections. Furthermore, we provide histological (HES) and light microscopical data to shed light on the structures and hypothetical function of sphaerodorid key morphological features. We provide fundamental details into the sphaerodorid morphology supporting a Phyllodocida ancestry of these enigmatic worms. However, the muscular arrangement and the presence of an axial muscular pharynx is similar to conditions observed in other members of the Errantia too. Furthermore, nervous system and muscle staining as well as SEM and histological observations of different types of tubercles indicate a homology of the so called microtubercles, present in the long-bodied sphaerodorids, to the dorsal cirri of other Errantia. The macrotubercles seem to represent a sphaerodorid autapomorphy based on our investigations. Therefore, our results allow comparisons concerning morphological patterns between Sphaerodoridae and other Phyllodocida and constitute a starting point for further comparative investigations to reveal the evolution of the remarkable Sphaerodoridae.

Le travail réalisé a pris en considération en premier lieu le droit international privé de l'UE, puis des aspects plus à proprement parler de « droit international procédural », en se concrétisant par une « systémisation » et une cartographie des voies de recours proposées au consommateur par le droit européen pour les litiges transfrontaliers. La première partie, concernant la loi applicable aux contrats internationaux du consommateur et le tribunal compétent dans le droit international privé de l'Union européenne, permit d'identifier les problématiques de droit international privé sous-jacentes aux contrats et aux litiges internationaux en matière de consommation, qui sont exacerbées dans le domaine du commerce électronique. La thèse traite deuxièmement la protection du consommateur par l'assouplissement et la simplification des procédures ordinaires et la protection du consommateur par des procédures collectives transfrontalières. Enfin, la thèse concerne la protection du consommateur par les procédures alternatives au contentieux ordinaire. / The thesis analyzes, under various aspects related to private international law and international civil procedure of the European Union, the status of the protection offered to the European consumers in their international contracts, in particular in relation to the objectives and in the context of the single market. The thesis is divided into three chapters. The first chapter deals with consumer protection in the international private law of European Union (regulation (UE) 1215/2012 and regulation (CE) 593/2008). The second chapter deals with the protection offered to consumers by european international civil procedure with reference to individual and collective litigations. The third chapter deals with alternative dispute resolution (ADR) and with on-line dispute resolution (ODR) and analyzes EU directive 2013/11/UE and regulation (EU) 524/2013. / La tesi analizza, sotto vari aspetti relativi al diritto internazionale privato e processuale dell’Unione Europea, lo stato della protezione offerta al consumatore europeo nei contratti e nelle controversie internazionali, con uno specifico riguardo alle tutele di carattere processuale e giurisdizionale. La tesi si articola in tre capitoli, che investono gli ambiti in cui tale tutela può esplicarsi, ovvero, l’individuazione di un foro e di una legge applicabile favorevoli agli interessi del consumatore, la semplificazione delle regole di procedura nelle controversie individuali internazionali, la possibilità di dare luogo a procedimenti transfrontalieri collettivi e la creazione di un sistema efficace di risoluzione delle controversie internazionali in via stragiudiziale anche on-line. Il primo capitolo ricostruisce dapprima il sistema di diritto internazionale privatodell'Unione europea in materia di contratti di consumo, dando conto del contesto politico ed economico in cui si inserisce ed alla cui luce devono esserne letti gli obiettivi. Vengono quindi individuati i principi e gli obiettivi che fondano le norme di diritto internazionale privato in materia di contratti internazionali del consumatore, le quali si caratterizzano per l’essere ispirate a finalità materiali, ovvero, volte a permettere di individuare un foro competente ed una legge applicabile che siano in grado di bilanciare tra loro le esigenze dei consumatori e degli operatori del mercato, con la conseguenza di favorire l’esplicarsi degli scambi commerciali e della concorrenza nel mercato unico. Ciò avviene garantendo al consumatore l’applicazione della legge e la competenza del foro a lui più prossimi, ovvero quelli del suo paese di residenza abituale (la cui coincidenza permette peraltro una riduzione dei costi delle liti transfrontaliere) e la prevedibilità delle soluzioni agli operatori del mercato.Il capitolo traccia quindi lo sviluppo normativo e giurisprudenziale delle norme di diritto internazionale privato europee in materia di contratti del consumatore, prendendo in particolare in considerazione le più recenti sentenze della Corte di Giustizia che, dal 2010 ad oggi, hanno ridefinito l’ambito di applicazione delle norme contenute nel regolamento (CE) 44/2001 (Bruxelles I), oggi rifuso nel regolamento (UE) 1215/2012 (Bruxelles I-bis), e nel regolamento (CE) 593/2008 (Roma I), insistendo sulla definizione del concetto, volutamente aleatorio, di “attività diretta” e sul suo ruolo di preminenza nel connettere la fattispecie contrattuale allo Stato della residenza del consumatore per permetterne la competenza dei giudici e l’applicabilità della relativa legge. Viene svolta infine una analisi critica sull’interpretazione fornita dalla Corte di Giustizia che rischia, nel concreto, di rendere le norme in oggetto applicabili in maniera variabile dai giudici nazionali, garantendo alle volte una tutela troppo estesa o troppo ristretta al consumatore, senza riguardo per l’esigenza di certezza giuridica degli operatori del mercato.