Climatic changes have induced striking altitudinal and latitudinal vegetation shifts throughout history. These shifts will almost certainly recur in the future; threatening other flora and fauna, and influencing climate feedback loops. Changes in the spatial distribution of vegetation are most conspicuous at physiognomically distinct ecotones, particularly between the subalpine forest and alpine tundra. Traditionally, ecological research has linked abiotic variables with the position of this ecotone (e.g., cold temperatures inhibit tree survival at high elevations). Thus, the prevailing assumption states that this ecotone is in equilibrium or quasi-equilibrium with the surrounding physical environment and that any dynamic shifts express direct linkages with the physical environment. This dissertation employs a landscape ecology approach to examine the abiotic and biotic ecological mechanisms most important in controlling tree establishment at this ecotone. The study site is on the western slopes of Mount Rainier, which was severely burned by a slash fire in 1930. Therefore, a crucial underlying assumption is that the ecological mechanisms controlling tree establishment are similar at disturbed and undisturbed sites. I exploited the use of 1970 CORONA satellite imagery and 2003 aerial photography to map 33 years of changes in arboreal vegetation. I created detailed maps of abiotic variables from a LIDAR-based DEM and biotic variables from classified remotely sensed data. I linked tree establishment patterns with abiotic and biotic variables in a GIS, and analyzed the correlations with standard logistic regression and logistic regression in the hierarchical partitioning framework at multiple spatial resolutions. A biotic factor (proximity to previously existing trees) was found to exert a strong influence on tree establishment patterns; equaling and in most cases exceeding the significance of the abiotic factors. The abiotic setting was more important at restricted spatial extents near the extreme upper limits of the ecotone and when analyzing coarse resolution data, but even in these cases proximity to existing trees remained significant. The strong overall influence of proximity to existing trees on patterns of tree establishment is unequivocal. If the underlying assumption of this dissertation is true, it challenges the long-held ecological assumption that vegetation in mountainous terrain is in equilibrium with and most strongly influenced by the surrounding physical environment.

Novel designs of three-dimensional patch antennas for beamdwidth enhancement and broadband peration have been proposed in this dissertation. For the beamwidth enhancement, the patch antenna with an inverted V-shaped patch has been proposed, and the antenna¡¦s radiation beamwidths have been broadened. Another beamwidth enhancement design using a three-dimensional square ground structure has also been demonstrated, and the 3-dB beamwidth of the CP radiation can reach more than 110¢X. In addition to the designs of beamwidth enhancement, the broadband patch antennas with a V-shaped patch, a W-shaped ground plane and a short probe-pin have been proposed. These novel structures can have significantly reduced probe-pin length and obtain a good impedance matching over a wide frequency range. Finally, a 1 ¡Ñ 2 broadband patch antenna array, suitable for base-station antenna applications for mobile communications systems, has also been studied.

beta-Bungarotoxin (beta-BuTx), the presynaptic neurotoxin purified from the venom of Bungarus multicinctus, consists of two dissimilar polypeptide subunits. A phospholipase A2 subunit named A chain, and a non-phospholipase A2 subunits named B chain. The A chain and B chain are covalently linked by one disulfide bridge. Although it has been widely accepted that the toxic effect of beta-BuTx is attributed to the disturbance of presynaptic transmitter release, however the inhibition of transmitter release by beta-BuTx is still obscure. Here we investigate the mechanism that mediates facilitation of transmitter release at the neuromuscular junction induced by beta-BuTx, using Xenopus nerve-muscle coculture. Application of beta-BuTx and isotoxins BM12, BM13 led to a marked increase in the frequency of spontaneous synaptic currents (SSCs) after a short period (12~18 min) of latency. The synaptic potentiation induced by these toxins was abolished when Ca2+ in the medium is substituted by Ba2+ (a potent phospholipase A2 inhibitor). Application of PLP-BM12 and PLP-BM13, which have been chemical-modification to lose their PLA2 activity from BM12 and BM13, failed to potentiate the transmitter release.

Reproduction de : Thèse de doctorat : Biologie cellulaire et moléculaire : Lyon 1 : 2004. / Titre provenant de l'écran titre. 256 réf. bibliogr.

Zugl.: Giessen, Univ., Diss., 2007

The RNA helicase Prp43p is one of the few members of the DEAH-box helicase family that is known to operate in more than one cellular process in Saccharomyces cerevisiae. With roles in ribosome biogenesis and pre-mRNA splicing, Prp43p may be important in maintaining a communication conduit between these two pathways. Our studies provide insights into how Prp43p function is regulated through the use of three cofactors, Ntr1p, Pfa1p, and Gno1p, all of which interact with Prp43p at different steps of pre-mRNA splicing or ribosome biogenesis. Each cofactor contains a unique G-patch domain and our data show that they associate with Prp43p in a mutually exclusive manner. A strong growth defect and RNA processing phenotypes are seen upon overexpression of Pfa1p due to the dominance of Pfa1p interaction with Prp43p. Moreover, excess Pfa1p precludes Prp43p from interacting with either 35S pre-rRNA or U6 snRNA, indicating this one cofactor can negatively regulate Prp43p recruitment into ribosome biogenesis and pre-mRNA splicing pathways, respectively. We have determined that Ntr1p and Gno1p are able to compete with one another for Prp43p occupancy. Similar to Ntr1p, we show that the G-patch domain of Gno1p contributes to its association with Prp43p. To further understand pathway specificity of Prp43p, we characterized conditional prp43 alleles with mutations C-terminal to the conserved RecA domains of Prp43p. These novel alleles affect pre-mRNA splicing and ribosome biogenesis, though none are mutually exclusive. Multiple prp43 alleles are deficient in tri-snRNP formation, a previously uncharacterized phenotype in prp43 mutants. The majority of our prp43 mutants display varying rRNA defects, with some alleles impacting ribosome biogenesis more severely or moderately than known prp43 ATPase mutants. To correlate the processing defects seen in each allele, we have determined the extent of association of the mutants with each G-patch protein. Altogether, our data support a working model for Prp43p in which its substrate specificity, activation, and cellular distribution is coordinated through the efforts of the three G-patch proteins in yeast and sheds light on potential mechanisms of general DExH/D helicase function and regulation. / text

Abstract Not Available.

Adiponectin (ADP) is an adipokine, which acts as an insulin sensitizing hormone. Recent studies have shown that adiponectin receptors (AdipoR1, AdipoR2) are present in the CNS; however, there is some debate as to whether or not ADP crosses the blood brain barrier (BBB). Circumventricular organs (CVO) are CNS sites outside the BBB, and thus represent sites at which circulating adiponectin may act to influence the CNS without having to cross the BBB. The subfornical organ (SFO) is a CVO that is responsive to a number of different circulating satiety signals including amylin, CCK, and ghrelin. We report here that the SFO also shows a high density of mRNA for both adiponectin receptors. These observations support the concept that the SFO may be a key player in sensing circulating ADP. To test the hypothesis that ADP influences the excitability of SFO neurons, we used current-clamp electrophysiology on dissociated SFO neurons to observe changes in membrane potential. ADP (10 nM) application effected the excitability of SFO neurons, where the cells either depolarized (8.9±0.9 mV, 21 of 97 cells) or hyperpolarized (-8.0±0.5 mV, 34 of 97 cells). Using single-cell RT-PCR we found that the majority of the responsive neurons expressed AdipoR1 or R2 and the non-responsive neurons expressed neither. In view of the recognized role of ADP in the regulation of energy balance, we next examined the effects of food deprivation for 48 hours on ADP signaling in the SFO. Our previous microarray analysis of SFO showed increases in AdipoR2 mRNA, with no significant change in AdipoR1 mRNA. We have also assessed the effects of such changes in receptor expression on ADP signaling in SFO neurons using calcium imaging and patch clamp techniques. In SFO neurons obtained from control animals, ADP induced increases in intracellular Ca2+ were observed in 25% of cells, while following food deprivation 0% of cells showed this response. Furthermore, 77% of neurons from starved animals showed clear depolarization, while no hyperpolarizing responses were observed. The results presented in this study suggest that adiponectin modulates the excitability of SFO neurons and that the response to ADP changes during starvation. / Thesis (Master, Neuroscience Studies) -- Queen's University, 2008-09-17 18:07:35.099

Many significant system extensions are hard to modularize. Consequently, their addition to a software system can jeopardize fundamental software engineering principles such as maintainability, understandability and evolvability. For example, the distributed Java Virtual Machine (dJVM) is a cluster aware implementation of a JVM in which distribution was retroactively added as an extension to an existing system. The prototype implementation of the dJVM relies on a patch file applied to IBM’s Jikes Research Virtual Machine (RVM), introducing distribution code into roughly 55% of the original 1166 Java files. In order to better determine the efficacy of modern modularization techniques such as aspect-oriented programming (AOP) in the context of system extensions, we offer up a case study based on distribution. The thesis of this work is that aspects can enhance extensibility of low-level system infrastructure software and be effectively integrated with existing software practices for introducing widespread change.

In arid landscapes limited resources are concentrated into fertile patches that allow greater productivity than would otherwise be expected. Fertile patches are created and maintained by processes that modify soil topography and interrupt the flow of resources across the landscape. These processes may be abiotic or biotic in origin. Species that modify, maintain or create habitat have been termed ecosystem engineers. One group of soil disturbing ecosystem engineers creates fertile patches by modifying soil microtopography through foraging for food and creating habitat. This thesis examines the effects of soil foraging animals on resource concentration in arid environments and how effects are moderated by species and landscape. Chapter 1 provides an overview of the importance of fertile patches in arid systems and describes previous research on the effects of ecosystem engineers in arid environments. Chapter 2 examines how fertile patch creation by a well-studied arid zone engineer, the ant, varies between ant species and landforms, demonstrating that while we can generalise about the effects of ant nests on water flow and nutrient levels, differences in soil type, nest density and ant species across sites are likely to moderate these effects. Chapters 3, 4 and 5 compare the engineering effects of reintroduced native species, the Greater bilby (Macrotis lagotis) and Burrowing bettong (Bettongia lesueur) with the Sand goanna (Varanus gouldii) and the invasive European rabbit (Oryctolagus cuniculus) across three Australian landforms, revealing differences between reintroduced and invasive engineers at the both patch and landscape scales. At the landscape scale, differences in pit densities and resource concentration in foraging pits between landforms suggest that fertile patch development is not uniform. Chapter 6 examines whether the structure of the pit or the presence of litter per se explains observed increases in plant germination in foraging pits, and compares temperature and soil moisture conditions in bilby and bettong pits to the soil surface. Chapter 7 is a review that builds upon previous chapters, published and unpublished literature of the potential for reintroductions of ecosystem engineers to restore ecosystem function in degraded environments. Chapter 8 summarises the research presented in this thesis and its implications, and suggests directions for future work.