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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
131

Effect of Livestock Species on Floral Resources and Pollinators in Low-Diversity Grasslands

Cutter, Jasmine Antonia Villamarin January 2020 (has links)
Livestock management influences the extent to which grazing lands provide resources for native species. We compared how livestock species – sheep or cattle – affected floral resources and bee and butterfly communities in low-diversity, post-Conservation Reserve Program (CRP) pastures managed with patch-burning. We sampled bees and butterflies three times per season 2017-2019 and counted flowering stems within 1 m of transects. Pastures grazed by sheep had significantly fewer flowers and significantly lower floral richness than cattle pastures. Native bees were three to sixteen times more abundant in cattle pastures compared to sheep. Butterfly communities were similar between grazing treatments, because agricultural-tolerant, habitat generalists comprised the majority of the butterfly community. Grassland-obligate butterflies comprised only 2% of observations. The dearth of grassland-obligate butterfly species and low native bee abundances suggest that post-CRP fields, especially those grazed by sheep, do not provide abundant and diverse floral resources for native bees and imperiled butterfly species.
132

Buněčné a molekulární mechanizmy aktivace teplotně citlivých TRP iontových kanálů / Cellular and molecular mechanisms of activation of thermally sensitive TRP ion channels

Máčiková, Lucie January 2020 (has links)
The transient receptor potential (TRP) are cation channels mostly permeable to both monovalent and divalent cations. ThermoTRP is a specific group of directly thermally activated TRP channels. The vanilloid transient receptor potential 3 (TRPV3) is an ion channel widely expressed in keratinocytes, that is implicated in the regulation of skin homeostasis, thermo- sensing, nociception and development of itch sensation. Our results show the importance of the cytoplasmic inter-subunit interface in the heat sensitivity of TRPV3. As there is a structural analogy within the vanilloid receptors, our hypothesis of the identified important region is supposed to be valid also for other thermally activated TRPV receptors (TRPV1, TRPV2 and TRPV4). We have proved that TRPV3 is a substrate for ERK1/2 protein kinase (kinase regulated by extracellular signal 1 and 2) and we have identified TRPV3 phosphorylation sites that may be direct targets for ERK1/2. Of these residues, threonine 264 has been shown to be the main phosphorylation site responsible for TRPV3 sensitization mediated by ERK kinase. In human keratinocytes, the phosphorylation might be physiologically and pathophysiologically important in processes of TRPV3 sensitization mediated by MAPK signaling pathway. The transient receptor potential ankyrin 1...
133

The Effect Of Olanzapine On The Synaptic Transmission Of The Dorsal Motor Nucleus Of The Vagus

January 2014 (has links)
Olanzapine, an atypical antipsychotic, alleviates symptoms of schizophrenia while producing fewer side effects compared to first generation antipsychotics. However, chronic usage remains problematic due to the propensity of olanzapine to induce weight gain and metabolic disturbances. Moreover, the cellular mechanisms underlying the metabolic side effects are poorly understood. The central nervous system (CNS) exerts both hormonal and neuronal control over whole body homeostasis. The dorsal motor nucleus of the vagus (DMV) participates in this regulation through modulation of the parasympathetic outflow to subdiaphragmatic organs. We hypothesized that olanzapine disrupts neurotransmission of the DMV, and thus contributes to the dysregulation of metabolism. We used whole-cell patch-clamp recordings from female C57BL/6J to assess the effect of olanzapine on DMV neurons. First, we investigated the effect of acute olanzapine administration on the activity of DMV neurons. Acute application of 10 µM olanzapine on DMV neurons induced both pre- and postsynaptic effects. Voltage-clamp recordings revealed that, in 5 out of 9 DMV neurons, excitatory inputs to DMV neurons were significantly increased by 71.6 ± 22.1%. In addition, in current-clamp mode, olanzapine induced a robust hyperpolarization from -49.00 ± 0.64 mV to -60.82 ± 2.78 mV. The hyperpolarization suppressed action potential firing. As a next step, we investigated the subchronic effect of olanzapine on the activity of DMV neurons. Daily subcutaneous injections were made for 20 days (5 mg/kg/day of olanzapine and vehicle). We did not find significant differences in body weight, blood glucose, and insulin or leptin levels. Subchronic administration of olanzapine generated presynaptic changes in DMV neurons. In treated animals, additional infusion of 10 µM olanzapine on DMV neurons significantly reduced excitatory neurotransmission by 41.0 ± 3.1% in 10 out of 17 neurons. Our findings indicate that olanzapine directly modulates the neuronal activity in DMV neurons, and could thus contribute to the metabolic disturbances seen in long-term treatments. / acase@tulane.edu
134

The Role of the Nucleosomal Acidic Patch in Histone Dimer Exchange

Gioacchini, Nathan 07 January 2022 (has links)
Eukaryotes organize their genomes by wrapping DNA around positively charged proteins called histones to form a structure known as chromatin. This structure is ideal for keeping the genome safe from damage, but also becomes an obstacle for the transcriptional machinery to access information stored in the DNA. To facilitate a balance between storage and accessibility, eukaryotes utilize a family of enzymes known as ATP-dependent chromatin remodelers to directly manipulate chromatin structure. The diverse activities of these chromatin remodeling enzymes range from simply sliding nucleosomes to reveal transcription start sites, to editing the composition of a nucleosome by exchanging canonical histones for histone variants. Chromatin remodeling enzymes recognize features of the nucleosome that activate their ATPase domains and enable proper remodeling function. One nuclear epitope that has been extensively studied is the nucleosomal acidic patch. This negatively charged region on the face of the nucleosome has been shown to be essential for remodeling enzymes like Chd1, ISWI, and INO80C. The chromatin remodeler SWR1C edits nucleosomes by removing the canonical histone H2A from nucleosomes and exchanges it for the histone variant H2A.Z, but the role of the acidic patch in this process has not been investigated. In this work, I showed that SWR1C has normal binding affinity to acidic patch mutant nucleosomes and retains ATPase stimulation but can no longer exchange dimers on this substrate. This work also identified a novel arginine anchor on the essential SWR1C subunit, Swc5, that binds specifically to the nucleosomal acidic patch. The data in this work suggest a mechanism where SWR1C engages nucleosomes and uses the Swc5 subunit to recognize the nucleosomal acidic patch to couple ATPase activity to histone dimer exchange.
135

Patch-Burn Grazing in Southwestern North Dakota: Assessing Above- and Belowground Rangeland Ecosystem Responses

Spiess, Jonathan Wesley January 2021 (has links)
Rangelands are heterogeneous working landscapes capable of supporting livestock production and biodiversity conservation, and heterogeneity-based rangeland management balances the potentially opposing production and conservation goals in these working landscapes. Within fire-dependent ecosystems, patch-burn grazing aims to create landscape patterns analogous to pre-European rangelands. Little work has tested the efficacy of patch-burn grazing in northern US Great Plains. We investigated patch contrast in above and belowground ecosystem properties and processes during the summer grazing seasons from 2017 ? 2020 on three patch-burn pastures stocked with cow-calf pairs and three patch-burn pastures stocked with sheep. We focused on vegetation structure, plant community composition, forage nutritive value, grazer selection, livestock weight gain, soil nutrient pools, soil microbial community composition, and decomposition activity. We used mixed-effect models and ordinations to determine whether differences: along the time since fire intensity gradient, between ecological sites, and between grazer types existed. Despite no significant shifts in the plant community, structural heterogeneity increased over time as the number of time since fire patches increased and was higher than homogeneously managed grasslands. Grazing livestock preferred recently burned patches where the available forage had a higher nutritive value and lower available biomass than surrounding patches at a given point in time. With the exception of 2018, livestock weight gains were consistent. Soil nutrient pools and microbial abundances differed more by ecological site than by the time since fire intensity gradient, and ecological sites exhibited similar nutrient and microbial responses to the time since fire intensity gradient. That belowground response variables were mostly resistant to patch-burn grazing is supportive of further use of this management, especially given the desirable results with aboveground response variables.
136

Neuroinflammatory conditions upregulate Piezo1 mechanosensitive ion channel in astrocytes

Jayasi, Jazmine 01 December 2021 (has links)
Neuroinflammation is prevalent in neurodegenerative diseases and plays a significant role in the central nervous system (CNS) innate immunity, which is the body’s first line of defense mechanisms against invading pathogens and injuries to maintain homeostasis. However, in neurodegenerative diseases, neuroinflammation becomes persistent alongside the subsequent damage to nearby neurons and affects CNS-resident immune glial cells, such as microglia and astrocytes. Accumulating evidence suggests that neuroinflammation is mainly characterized by the excessive activation of glial cells, thus causing abnormal changes in their microenvironment and release soluble factors that can promote or inhibit neuroinflammation. Currently, there is no effective treatment to cure these progressive neurological disorders. Therefore, it is critical to understand how neuroinflammation affects astroglia cell function and their biomechanical properties that change their behavior throughout disease progression. Astrocytes are the most predominant glial cell in the CNS and are critical in the development and maintenance of neuroinflammatory disorders. To date, very little is known regarding the role and specific function of Piezo1 mechanosensitive ion channel (MSC) in the CNS. Recently, Piezo1 expression was found to be upregulated in Lipopolysaccharide (LPS)-induced neuroinflammation in mouse astrocyte cultures. However, it is unknown whether the aberrant mechanical environment in astrocytes interplay with the mechanosensory function of Piezo1 and its current activity in neuroinflammatory conditions. In this study, we investigated Piezo1 mechanosensitive ionic currents by performing in vitro patch-clamp electrophysiology and calcium imaging. Our preliminary studies revealed that astrocytes derived from the mouse cerebellum stimulated with LPS or Piezo1 agonist, Yoda1, increased Ca2+ influx and further augmented when treated concurrently. We also found that electrophysiology recordings showed changes in mechanosensitive ionic currents and were comparable with our calcium imaging data indicating that MSCs are involved in neuroinflammation. Therefore, we postulated that Piezo1, a non-selective cation MSC that opens in response to mechanical force is a key mechanosensor involved in neuroinflammation by altered mechanical signals in C8-S astrocytes. Using an in vitro system of Mouse C8-S (Astrocyte type II clone), the goal of this study was to investigate if neuroinflammatory conditions upregulate Piezo1 calcium influx and current activity. We show that astrocytic Piezo1 regulates mechanotransducive release of ATP by controlling the mechanically induced calcium influx and current activation in LPS-induced astrocytes. Additionally, Piezo1 antagonist, GsMTx4 and Piezo1 siRNA significantly reduced the LPS-induced current, indicating that Piezo1 is involved in neuroinflammation. Our findings demonstrate that the activity of Piezo1 stimulated by neuroinflammatory conditions may be significant for the development of therapeutics to prevent or treat neuroinflammatory disorders and diseases.
137

Meshed Patch Antennas Integrated on Solar Cell - A Feasibility Study and Optimization

Turpin, Timothy W. 01 May 2009 (has links)
This thesis work presents the feasibility of integrating meshed patch antennas directly onto the solar cell assembly to save valuable surface real estate of a small satellite. The solar cell cover glass is used as the substrate for the patch antenna. The antenna topology is chosen to be a meshed patch so that it is transparent to light to ensure the proper operation of solar cells. We found that although there is a compromise between the antenna efficiency and see-through percentage, one is able to optimize the antenna by carefully designing the mesh. To verify the design and integration, a meshed antenna operating at around 2.3 GHz is printed with conductive ink on a plastic substrate and placed on-top of solar cells attached to an aluminum ground plane. The printed solar cell antenna is measured with Nearfield Systems Inc. spherical near-field range and the measurements agree well with the design.
138

A Linearly and Circularly Polarized Active Integrated Antenna

Khoshniat, Ali 01 May 2011 (has links)
This thesis work presents a new harmonic suppression technique for microstrip patch antennas. Harmonic suppression in active integrated antennas is known as an effective method to improve the efficiency of amplifiers in transmitter side. In the proposed design, the antenna works as the radiating element and, at the same time, as the tuning load for the amplifier circuit that is directly matched to the antenna. The proposed active antenna architecture is easy to fabricate and is symmetric, so it can be conveniently mass-produced and designed to have circular polarization, which is preferred in many applications such as satellite communications. The antenna simulations were performed using Ansoft High Frequency System Simulator (HFSS) and all amplifier design steps were simulated by Advanced Design System (ADS). The final prototypes of the linearly polarized active integrated antenna and the circularly polarized active integrated antenna were fabricated using a circuit board milling machine. The antenna radiation pattern was measured inside Utah State University's anechoic chamber and the results were satisfactory. Power measurements for the amplifiers' performance were carried out inside the chamber and calculated by using the Friis transmission equation. It is seen that a significant improvement in the efficiency is achieved compared to the reference antenna without harmonic suppression. Based on the success in the single element active antenna design, the thesis also presents a feasibility of applying the active integrated antenna in array configuration, in particular, in scanning array design to yield a low-profile, low-cost alternative to the parabolic antenna transmitter of satellite communication systems.
139

Metamaterial Enhanced Wireless Power Transmission System

Heffernan, Travis Jade 01 July 2013 (has links) (PDF)
Nikolai Tesla's revolutionary experiments demonstrated the possible benefits of transmitting power wirelessly as early as 1891. Applications for the military, consumers, emergency personnel, remote sensors, and others use Tesla’s discovery of wireless power. Wireless power transmission (WPT) has the potential to be a common source of consumable energy, but it will only receive serious consideration if the transmit and receive systems are extremely efficient and capable of delivering usable amounts of power. Research has been conducted to improve the efficiency and performance of nearly every aspect of WPT systems, but the relatively new field of metamaterials (MTMs) has yet to play a dominate role in improving system performance. A gradient index (GRIN) MTM lens was designed using Ansoft’s High Frequency Structure Simulator (HFSS) to improve antenna gain and thereby increase WPT system performance. A simple WPT demonstration system using microstrip patch antennas (MPAs) confirmed the benefits of the GRIN MTM lens. The WPT demonstration system, MPAs, and GRIN MTM lens were constructed and experimentally tested near 2.45 GHz. The theoretical and experimental gain improvement of the MPA due to the GRIN MTM lens is 5.91 dB and 7.06 dB, respectively.
140

Régulation présynaptique des interneurones GABAergiques par le récepteur u-opioïde dans l'aire tegmentaire ventrale

Bergevin, Annie January 2001 (has links)
Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.

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