This study, conducted in one local authority in England, set out to investigate how and why ‘Structured Teaching’ is implemented for children with autism and learning difficulties who are educated in special schools in one local authority in England. Structured Teaching is the educational component of the comprehensive approach ‘Treatment and Education for Autistic and Related Communication handicapped CHildren’, better known as the TEACCH approach. Structured Teaching aims to promote independence and self-esteem, manage behaviours and, in so doing, facilitate learning. Despite its popularity, there has been little research in relation to the approach as it is implemented in the United Kingdom. In addition, the need for eclectic educational practice is widely documented, given the wide range of individual needs and strengths of learners with autism. Yet despite schools implementing a diverse range of approaches, knowledge of how and why teachers select combinations of approaches is limited. This investigation aimed therefore to: 1. Identify which Structured Teaching strategies are implemented, in what ways and for what purposes. 2. Identify which other approaches are used in combination with Structured Teaching and for what purposes. 3. Explore factors which underpin teachers’ decisions in selecting and combining approaches. An early literature review established key principles, purposes, definitions and concepts associated with Structured Teaching, as determined by those who developed the TEACCH approach to autism. This enabled the researcher to evaluate whether Structured Teaching was being implemented in the ways intended by those who developed the approach. A further literature review explored the existing research evidence-base for Structured Teaching and revealed gaps in that evidence, both methodologically and in relation to outcomes for children. A positivist approach which measures children’s behaviours has resulted in Structured Teaching being identified as an ‘evidence-based approach’. However, that evidence neglects to consider the perceptions of those who implement the approach and does not consider in depth other outcomes for children such as the effect upon their wellbeing and readiness to learn. This investigation was therefore designed to explore the gaps in the existing research evidence in order to better understand how and why the approach is implemented. An initial survey questionnaire, distributed to five special schools in one local authority in England, found that all components of Structured Teaching were being implemented. The results revealed that a predominant perception of outcomes for children was linked to their wellbeing. A variety of other classroom approaches were also identified by respondents and again linked to children’s wellbeing. Subsequently, an interpretative case study approach was designed to gather qualitative insights into classroom practices in relation to Structured Teaching combined with other approaches. Multiple case studies included: two key stage two classes in one special school; one key stage two class, together with one contrasting class for children in their early years, in another special school in a neighbouring town. Fieldwork took place over four school terms. Iterative analysis of interviews and classroom observations revealed that Structured Teaching is implemented as a flexible framework, responsive to individual needs and strengths. Within this framework, a combination of other approaches is implemented. Decision-making is underpinned by knowing each child as an individual and with a priority focus upon children’s wellbeing. The analysis and synthesis of the case studies result in a new model, which reflects the ‘mindful blending’ of approaches within a framework of Structured Teaching. The results of this investigation complement the existing research evidence-base. Future research might best be conducted by adopting a mixed-methods approach, combining positivist with interpretivist methodology. This would enhance the research evidence. Measurable behaviours would reveal what children do as a result of classroom practices, whilst insights of practitioners may shed light on potential reasons for why. In addition, an interpretive approach might also usefully gather the insights of those who are at the receiving end of a blend of approaches, that is, the children and their families. Finally, future research might test the usefulness of the ‘mindful blending’ model in order to inform and enhance educational approaches for children with autism.

The concentration of calprotectin in feces (fCal) is a clinically useful marker of chronic gastrointestinal inflammation in humans and dogs. No commercial assay is widely available to measure fCal in small animal medicine, to date. Thus, we verified the immunoturbidimetric fCAL turbo assay (Bühlmann) of fCal for canine and feline fecal extracts by determining linearity, spiking and recovery, and intra-assay and inter-assay variability. We determined RIs, temporal variation over 3 mo, and effect of vaccination and NSAID treatment. Observed:expected (O:E) ratios (x̄ ± SD) for serial dilutions of feces were 89– 131% (106 ± 9%) in dogs and 77–122% (100 ± 12%) in cats. For spiking and recovery, the O:E ratios were 90–118% (102 ± 11%) in dogs and 83–235% (129 ± 42%) in cats. Intra- and inter-assay CVs for canine samples were ≤19% and ≤7%, and for feline samples ≤22% and ≤21%. Single-sample RIs were <41 μg/g for dogs and <64 μg/g for cats. With low reciprocal individuality indices, using population-based fCal RIs is appropriate, and moderate fCal changes between measurements (dogs 44.0%; cats: 43.2%) are considered relevant. Cats had significant (but unlikely relevant) fCal increases post-vaccination. Despite individual fCal spikes, no differences were seen during NSAID treatment. The fCAL turbidimetric assay is linear, precise, reproducible, and sufficiently accurate for measuring fCal in dogs and cats. Careful interpretation of fCal concentrations is warranted in both species during the peri-vaccination period and for some patients receiving NSAID treatment.

This study used Interpretative Phenomenological Analysis (IPA) to investigate the experiences of African immigrant mothers living in the UK with a child diagnosed with an Autism Spectrum Disorder (ASD). Six mothers took part in one-off semi-structured interviews. The results indicated five themes: caring for a child we didn’t expect, the pain of stigma and rejection, making sense of our child’s difficulties and diagnosis, negotiating conflicting belief systems and faith as “key”. Many aspects of the mothers’ experiences appear related to their position as immigrants from cultures with very different belief systems regarding child development and disability. Stigma, blame and social isolation appeared to compound the difficulties they experienced. Conflicts between African cultural beliefs and a western, medical understanding of ASD, appeared to create a feeling of cognitive dissonance for the mothers. The strategies they used to negotiate this appear to map onto Berry’s (2005) acculturation strategies, suggesting the experience of having a child with ASD impacts upon the acculturation process. Implications for clinical practice and policy are discussed, including the importance of raising awareness of ASD among immigrant communities, supporting parents to integrate conflicting belief systems and facilitating the development of peer-support groups within minority communities.

A complexidade dos sistemas integrados em chips bem como a arquitetura de processadores comerciais vem crescendo dramaticamente nos últimos anos. Com isto, a dificuldade de avaliarmos a suscetibilidade às falhas em decorrência da incidência de partículas espaciais carregadas nestes dispositivos cresce com a mesma taxa. Este trabalho apresenta uma análise comparativa da susceptibilidade à erros de software em um microprocessador embarcado ARM Cortex-A9 single core de larga escala comercial, amplamente utilizado em aplicações críticas, executando um conjunto de 11 aplicações desenvolvidas para um ambiente bare metal e para o sistema operacional Linux. A análise de soft errors é executada por injeção de falhas na plataforma de simulação OVPSim juntamente com o injetor OVPSim-FIM, capaz de sortear o momento e local de injeção de uma falha. A campanha de injeção de falhas reproduz milhares de bit-flips no banco de registradores do microprocessador durante a execução do conjunto de benchmarks que possuem um comportamento de código diverso, desde dependência de fluxo de controle até aplicações intensivas em dados. O método de análise consiste em comparar execuções da aplicação onde falhas foram injetadas com uma execução livre de falhas. Os resultados apresentam a taxa de falhas que são classificadas em: mascaradas (UNACE), travamento ou perda de controle de fluxo (HANG) e erro nos resultados (SDC). Adicionalmente, os erros são classificados por registradores, separando erros latentes por sua localização nos resultados e por exceções detectadas pelo sistema operacional, provendo novas possibilidades de análise para um processador desta escala. O método proposto e os resultados obtidos podem ajudar a orientar desenvolvedores de software na escolha de diferentes arquiteturas de código, a fim de aprimorar a tolerância à falhas do sistema embarcado como um todo. / The complexity of integrated system on-chips as well as commercial processor’s architecture has increased dramatically in recent years. Thus, the effort for assessing the susceptibility to faults due to the incidence of spatial charged particles in these devices has growth at the same rate. This work presents a comparative analysis of soft errors susceptibility in the commercial large-scale embedded microprocessor ARM Cortex-A9 single core, widely used in critical applications, performing a set of 11 applications developed for a bare metal environment and the Linux operating system. The soft errors analysis is performed by fault injection in OVPSim simulation platform along with the OVPSim-FIM fault injector, able to randomly select the time and place to inject the fault. The fault injection campaign reproduces thousands of bit-flips in the microprocessor register file during the execution of the benchmarks set, with a diverse code behavior ranging from control flow dependency to data intensive applications. The analysis method is based on comparing applications executions where faults were injected with a fault-free implementation. The results show the error rate classified by their effect as: masked (UNACE), crash or loss of control flow (HANG) and silent data corruption (SDC); and by register locations. By separating latent errors by its location in the results and exceptions detected by the operating system, one can provide new better observability for a large-scale processor. The proposed method and the results can guide software developers in choosing different code architectures in order to improve the fault tolerance of the embedded system as a whole.

La Polyarthrite Rhumatoïde est caractérisée par une synovite à l'origine de lésions progressives ostéo-articulaires induites par les formes réactives de l'oxygène (ROS) produites par la NADPH oxydase des polynucléaires neutrophiles (PMN). La NADPH oxydase des phagocytes, est formée d'un centre catalytique membranaire, le cytochrome b558, sur lequel vient s'associer des protéines cytosoliques régulatrices (p67phox, p47phox, p40phox et Rac1/2). Nous avons étudié la spécificité de l'interaction entre la (6-phosphofructokinase 2) et de la 6PGDH (6-phosphogluconate déshydrogénase) et la NADPH oxydase des PMN. D'autre part, nous avons caractérisé les domaines de l'hétérocomplexe S100A8/A9 impliqués dans l'activation de la NADPH oxydase phagocytaire. Par ailleurs, une étude de la signature protéique dans le liquide synovial a été menée afin de rechercher l'empreinte de l'activation du PMN dans la PR.Après stimulation par le PMA, la 6PGDH et la PFK2 co-imunoprécipitent avec les facteurs cytosoliques p67phox, p47phox and p40phox. Les expériences de microscopie confocale suggèrent une co-localisation de ces deux enzymes du métabolisme du glucose avec la NADPH oxydase, dans des micro-domaines membranaires : les radeaux lipidiques. La 6PGDH est impliquée dans l'activation de la NADPH oxydase phagocytaire en élevant la concentration du NADPH cytosolique mais également en augmentant l'affinité de cette enzyme pour son substrat, le NADPH. PFK2 est l'enzyme majeure de la régulation de la glycolyse, voie est essentielle pour la production d'ATP du PMN. L'utilisation du complexe S100A8/A9 et de protéines chimères de fusion nous a permis de révéler que la partie C-terminale de S100A8 est impliquée dans la liaison avec le cytochrome b558 et l'activation de la NADPH oxydase phagocytaire. In vivo, le profil protéique du liquide articulaire de PR a révélé l'empreinte de l'activation du PMN dans cette pathologie avec une surexpression des protéines S100A8 et S100A9. Une production ectopique de S100A8/A9 par les synoviocytes de type fibroblastique a été mise en évidence.En conclusion, la 6PGDH, la PFK2 et l'hétérodimère S100A8/A9 sont de nouveaux partenaires d'activation de la NADPH oxydase des phagocytes. Dans la PR, l'activation des PMNs conduit à la sécrétion de S100A8/A9 qui semblent constituer à la fois des biomarqueurs pertinents, mais également des cibles thérapeutiques potentielles.

A complexidade dos sistemas integrados em chips bem como a arquitetura de processadores comerciais vem crescendo dramaticamente nos últimos anos. Com isto, a dificuldade de avaliarmos a suscetibilidade às falhas em decorrência da incidência de partículas espaciais carregadas nestes dispositivos cresce com a mesma taxa. Este trabalho apresenta uma análise comparativa da susceptibilidade à erros de software em um microprocessador embarcado ARM Cortex-A9 single core de larga escala comercial, amplamente utilizado em aplicações críticas, executando um conjunto de 11 aplicações desenvolvidas para um ambiente bare metal e para o sistema operacional Linux. A análise de soft errors é executada por injeção de falhas na plataforma de simulação OVPSim juntamente com o injetor OVPSim-FIM, capaz de sortear o momento e local de injeção de uma falha. A campanha de injeção de falhas reproduz milhares de bit-flips no banco de registradores do microprocessador durante a execução do conjunto de benchmarks que possuem um comportamento de código diverso, desde dependência de fluxo de controle até aplicações intensivas em dados. O método de análise consiste em comparar execuções da aplicação onde falhas foram injetadas com uma execução livre de falhas. Os resultados apresentam a taxa de falhas que são classificadas em: mascaradas (UNACE), travamento ou perda de controle de fluxo (HANG) e erro nos resultados (SDC). Adicionalmente, os erros são classificados por registradores, separando erros latentes por sua localização nos resultados e por exceções detectadas pelo sistema operacional, provendo novas possibilidades de análise para um processador desta escala. O método proposto e os resultados obtidos podem ajudar a orientar desenvolvedores de software na escolha de diferentes arquiteturas de código, a fim de aprimorar a tolerância à falhas do sistema embarcado como um todo. / The complexity of integrated system on-chips as well as commercial processor’s architecture has increased dramatically in recent years. Thus, the effort for assessing the susceptibility to faults due to the incidence of spatial charged particles in these devices has growth at the same rate. This work presents a comparative analysis of soft errors susceptibility in the commercial large-scale embedded microprocessor ARM Cortex-A9 single core, widely used in critical applications, performing a set of 11 applications developed for a bare metal environment and the Linux operating system. The soft errors analysis is performed by fault injection in OVPSim simulation platform along with the OVPSim-FIM fault injector, able to randomly select the time and place to inject the fault. The fault injection campaign reproduces thousands of bit-flips in the microprocessor register file during the execution of the benchmarks set, with a diverse code behavior ranging from control flow dependency to data intensive applications. The analysis method is based on comparing applications executions where faults were injected with a fault-free implementation. The results show the error rate classified by their effect as: masked (UNACE), crash or loss of control flow (HANG) and silent data corruption (SDC); and by register locations. By separating latent errors by its location in the results and exceptions detected by the operating system, one can provide new better observability for a large-scale processor. The proposed method and the results can guide software developers in choosing different code architectures in order to improve the fault tolerance of the embedded system as a whole.

La Polyarthrite Rhumatoïde est caractérisée par une synovite à l’origine de lésions progressives ostéo-articulaires induites par les formes réactives de l’oxygène (ROS) produites par la NADPH oxydase des polynucléaires neutrophiles (PMN). La NADPH oxydase des phagocytes, est formée d’un centre catalytique membranaire, le cytochrome b558, sur lequel vient s’associer des protéines cytosoliques régulatrices (p67phox, p47phox, p40phox et Rac1/2). Nous avons étudié la spécificité de l’interaction entre la (6-phosphofructokinase 2) et de la 6PGDH (6-phosphogluconate déshydrogénase) et la NADPH oxydase des PMN. D’autre part, nous avons caractérisé les domaines de l’hétérocomplexe S100A8/A9 impliqués dans l’activation de la NADPH oxydase phagocytaire. Par ailleurs, une étude de la signature protéique dans le liquide synovial a été menée afin de rechercher l’empreinte de l’activation du PMN dans la PR.Après stimulation par le PMA, la 6PGDH et la PFK2 co-imunoprécipitent avec les facteurs cytosoliques p67phox, p47phox and p40phox. Les expériences de microscopie confocale suggèrent une co-localisation de ces deux enzymes du métabolisme du glucose avec la NADPH oxydase, dans des micro-domaines membranaires : les radeaux lipidiques. La 6PGDH est impliquée dans l’activation de la NADPH oxydase phagocytaire en élevant la concentration du NADPH cytosolique mais également en augmentant l’affinité de cette enzyme pour son substrat, le NADPH. PFK2 est l’enzyme majeure de la régulation de la glycolyse, voie est essentielle pour la production d’ATP du PMN. L’utilisation du complexe S100A8/A9 et de protéines chimères de fusion nous a permis de révéler que la partie C-terminale de S100A8 est impliquée dans la liaison avec le cytochrome b558 et l’activation de la NADPH oxydase phagocytaire. In vivo, le profil protéique du liquide articulaire de PR a révélé l’empreinte de l’activation du PMN dans cette pathologie avec une surexpression des protéines S100A8 et S100A9. Une production ectopique de S100A8/A9 par les synoviocytes de type fibroblastique a été mise en évidence.En conclusion, la 6PGDH, la PFK2 et l’hétérodimère S100A8/A9 sont de nouveaux partenaires d’activation de la NADPH oxydase des phagocytes. Dans la PR, l’activation des PMNs conduit à la sécrétion de S100A8/A9 qui semblent constituer à la fois des biomarqueurs pertinents, mais également des cibles thérapeutiques potentielles. / Rheumatoid Arthritis (RA) is caused by an inflammation of the synovial membrane leading to progressive joint destruction and deformation, related to the production NADPH oxidase related-reactive oxygen species (ROS) production. The phagocyte NADPH oxidase is a multi-protein complex formed by a catalytic core, i.e. the transmembrane cytochrome b558 and cytosolic regulators (p67phox, p47phox, p40phox and Rac1/2). We aimed at better analyzing the NADPH oxidase activation through the evaluation of the specificity of the interaction with 6PGDH or PFK2 and through the further analysis of the association with the S100A8/A9 heterocomplex. The RA-specific protein profiling was conducted in order to determine whether a PMN activation fingerprint could be revealed among RA specific proteins.Upon PMA stimulation, both 6PGDH and PFK2 co-imunoprecipitated with cytosolic factors p67phox, p47phox and p40phox. At the plasma membrane level, confocal microscopy experiments suggested a co-localization of either 6PGDH or PFK2 with the phagocyte NADPH oxidase in lipid rafts. 6PGDH enhanced the phagocyte NADPH oxidase activity by both improving the availability of cytosolic NADPH content and by increasing the affinity of the NADPH oxidase for its substrate. PFK2 also augmented the NADPH oxidase activity. PFK2 modulated the ATP concentration available for the phosphorylation of the phagocyte NADPH oxidase components and for the NDP Kinase related-Rac activation. The generation of truncated S100A8/S100A9 heterodimer chimera could reveal that the C-terminal region of S100A8 is involved in both the interaction and the activation of the phagocyte NADPH oxidase.In vivo, synovial fluid of RA patients was remarkably labelled with the PMN activation fingerprint. S100A8 and S100A9 proteins clearly distinguished RA synovial fluid from osteoarthritis and non RA-synovial fluids. An ectopic production of S100A8/S100A9 was shown in RA fibroblast like synoviocyte.In conclusion, 6PGDH, PFK2 and S100A8/A9 proteins are surrogate activating partners of the phagocyte NADPH oxidase. In RA, the activation of PMNs leads to the release of S100A8/A9 proteins which may constitute interesting biomarkers and promising therapeutic targets.

A complexidade dos sistemas integrados em chips bem como a arquitetura de processadores comerciais vem crescendo dramaticamente nos últimos anos. Com isto, a dificuldade de avaliarmos a suscetibilidade às falhas em decorrência da incidência de partículas espaciais carregadas nestes dispositivos cresce com a mesma taxa. Este trabalho apresenta uma análise comparativa da susceptibilidade à erros de software em um microprocessador embarcado ARM Cortex-A9 single core de larga escala comercial, amplamente utilizado em aplicações críticas, executando um conjunto de 11 aplicações desenvolvidas para um ambiente bare metal e para o sistema operacional Linux. A análise de soft errors é executada por injeção de falhas na plataforma de simulação OVPSim juntamente com o injetor OVPSim-FIM, capaz de sortear o momento e local de injeção de uma falha. A campanha de injeção de falhas reproduz milhares de bit-flips no banco de registradores do microprocessador durante a execução do conjunto de benchmarks que possuem um comportamento de código diverso, desde dependência de fluxo de controle até aplicações intensivas em dados. O método de análise consiste em comparar execuções da aplicação onde falhas foram injetadas com uma execução livre de falhas. Os resultados apresentam a taxa de falhas que são classificadas em: mascaradas (UNACE), travamento ou perda de controle de fluxo (HANG) e erro nos resultados (SDC). Adicionalmente, os erros são classificados por registradores, separando erros latentes por sua localização nos resultados e por exceções detectadas pelo sistema operacional, provendo novas possibilidades de análise para um processador desta escala. O método proposto e os resultados obtidos podem ajudar a orientar desenvolvedores de software na escolha de diferentes arquiteturas de código, a fim de aprimorar a tolerância à falhas do sistema embarcado como um todo. / The complexity of integrated system on-chips as well as commercial processor’s architecture has increased dramatically in recent years. Thus, the effort for assessing the susceptibility to faults due to the incidence of spatial charged particles in these devices has growth at the same rate. This work presents a comparative analysis of soft errors susceptibility in the commercial large-scale embedded microprocessor ARM Cortex-A9 single core, widely used in critical applications, performing a set of 11 applications developed for a bare metal environment and the Linux operating system. The soft errors analysis is performed by fault injection in OVPSim simulation platform along with the OVPSim-FIM fault injector, able to randomly select the time and place to inject the fault. The fault injection campaign reproduces thousands of bit-flips in the microprocessor register file during the execution of the benchmarks set, with a diverse code behavior ranging from control flow dependency to data intensive applications. The analysis method is based on comparing applications executions where faults were injected with a fault-free implementation. The results show the error rate classified by their effect as: masked (UNACE), crash or loss of control flow (HANG) and silent data corruption (SDC); and by register locations. By separating latent errors by its location in the results and exceptions detected by the operating system, one can provide new better observability for a large-scale processor. The proposed method and the results can guide software developers in choosing different code architectures in order to improve the fault tolerance of the embedded system as a whole.

In this exam project the investigation has been done to determine, which profits that can be made by switching an internal bus between two chips, one FPGA and a processor, to an internal bus implemented on only one chip, System on Chip. The work is based on measurements made in real time in Xilinx’s development tools on different buses, AXI4 and AXI4-Light connected to AXI3. The port that is used is FPGA’s own GP-port. Besides measuring the time of transactions also physical aspects have been investigated in this project: space, costs and time. Based on those criteria a comparison to the original construction was made to determine which benefits that can be achieved. The work has shown a number of results that are in comparison with the original construction. The System on Chip has turned out to be a better solution in most cases. When using the AXI4-Light-bus the benefits were not as obvious. Cosmic radiation, temperature or humidity are beyond the scope of this investigation. In the work the hypothetic deductive method has been used to prove that the System on Chip is faster than the original design. In this method three statements must be set up against each other; one statement that ought to be true, one statement that is a contradiction and a conclusion of what is proved. The pre-study pointed out that the System on Chip is a faster solution than the original construction. The method is useful since it proves that the pre-study is comparable to the measured results. / I detta examensarbete har undersökningar gjorts för att fastställa vilka vinster som går att göra genom att byta en internbuss mellan två chip, en FPGA och en processor, mot en intern buss implementerat på ett enda chip, System on Chip. Arbetet bygger på mätningar gjorda i realtid i Xilinx utvecklingsverktyg på olika bussar, AXI4 och AXI4‑Lite som är kopplade internt mot AXI3. Den port som används är FPGAs egen GP‑port. Förutom att mäta överföringshastigheterna, har även fysiska aspekter som utrymme, kostnader och utvecklingstid undersökts. Utifrån dessa kriterier har en jämförelse gjorts med den befintliga konstruktionen för att fastställa vilka vinster som går att uppnå. Arbetet har resulterat i ett antal resultat som är ställda mot de förutsättningar som fanns i den ursprungliga lösningen. I de flesta fall visar resultatet att ett System on Chip är en bättre lösning. De fall som var tveksamma var vid viss typ av överföring med AXI4‑Lite bussen. I arbetet har inte undersökning av kosmisk strålning, temperatur eller luftfuktighet betraktas. I arbetet med att försöka att bevisa att ett System on Chip är snabbare än den ursprungliga uppsättningen har utvecklingsmetoden hypotetisk deduktiv använts. Denna metod bygger på att man från början sätter upp ett påstående, som man förutsätter är sant, följt av en konjunktion, som inte får inträffa, för att slutligen dra en slutsats, som konstaterar fakta. Eftersom fakta som lästes in i början av arbetet pekade på att ett System on Chip var en snabbare och billigare lösning kändes metoden användbar. Under arbetets gång har det visat sig vara en bra metod som också ger ett resultat där sannolikheten för att det är en snabbare lösning ökar. Däremot säger inte metoden att det är helt säkert att den i alla situationer är bättre, vilket kan ändras om man använder andra förutsättningar eller tar med andra aspekter.

This thesis deals with the modifications of the hardware design and operating systems of the ZedBoard multi-core platform so that both ARM Cortex A9 processor cores included in SoC Zynq7000 can be used. It analyses the general issue of the multi-core environment and the core functions of the kernel and the operating system. It describes selected means of implementation ZedBoard and FreeRTOS. In the implementation section, specific steps are demonstrated to convert a single-core operating system to a multi-core system but also steps required to run two different operating systems on two processor cores. In the last section all achieved results are summarized.