Si les différentes composantes du syndrome métabolique (SM) sont susceptibles d’affecter le métabolisme protéique et des acides aminés (AA), les données disponibles sont peu nombreuses et souvent contradictoires, du fait de l’hétérogénéité de présentation de ce syndrome et des limites des approches classiques d’investigation du métabolisme azoté. Ce travail de thèse met à profit une nouvelle approche isotopomique, s’appuyant sur la mesure de l’abondance naturelle des isotopes stables de l’azote (δ15N) et du carbone (δ13C) dans les protéines et AA tissulaires pour identifier les altérations du métabolisme protéique survenant lors de l’induction nutritionnelle d’un SM chez le rat. Nos résultats permettent dans un premier temps de valider expérimentalement les prédictions d’un modèle multi-compartimental développé dans le laboratoire et montrant que les δ15N reflètent l’orientation différentielle des AA entre les voies anaboliques (protéosynthèse) et cataboliques (oxydation). Nous avons également montré que sous certaines conditions, les δ13C permettent d’estimer la part des carbones des AA et protéines tissulaires provenant respectivement des protéines, glucides et lipides alimentaires, renseignant ainsi sur la flexibilité métabolique des individus. Les mesures de δ15N et δ13C dans les protéines et AA, seules ou combinées à la mesure des taux de synthèse protéique après administration d’eau deutérée, nous ont ensuite permis de mettre en évidence les modifications du métabolisme protéique et des AA survenant lors de l’exposition périnatale et post-sevrage à un régime gras et sucré, ainsi que celles associées à des différences de sensibilité individuelles à l’induction d’un syndrome métabolique par ce même type de régime. Ces altérations sont tissu-spécifiques et diffèrent selon qu’elles proviennent uniquement de différences de sensibilité individuelle au régime ou qu’elles sont également attribuables à des différences d’équilibre glucido-lipidique dans l’alimentation. L’ensemble de nos résultats montrent que l’apparition d’un SM est associée à des réorientations du métabolisme des AA entre les voies anaboliques et d’oxydation, affectant de façon différente le foie, le muscle, l’intestin et le tissu adipeux, et à une altération de la flexibilité métabolique dans le muscle. Ces travaux ouvrent la voie à des études chez l’Homme, s’appuyant sur les mesures de δ15N et δ13C dans des pools accessibles. / Although the different components of the metabolic syndrome (MS) are likely to affect protein and amino acid (AA) metabolism, the available data are few and often contradictory, due to the heterogeneity of presentation of this syndrome and the limitations of classical approaches to investigate nitrogen metabolism. The present thesis work uses a novel isotopomic approach, based on the measurement of the natural abundance of stable isotopes of nitrogen (δ15N) and carbon (δ13C) in tissue proteins and AA to identify alterations in protein metabolism occurring during the nutritional induction of MS in rats. Our results allow to validate experimentally the predictions of a multi-compartimental model developed in the laboratory and showing that the δ15N reflects the differential orientation of AA between anabolic (proteosynthesis) and catabolic (oxidation) pathways. We have also shown that under certain conditions, the δ13C can allow to estimate the proportion of carbons in AA and tissue proteins issuing from dietary proteins, carbohydrates and lipids respectively, thus providing information on the metabolic flexibility of individuals. The measurements of δ15N and δ13C in proteins and AA, alone or combined with the measurement of protein synthesis rates after administration of deuterated water, then allowed us to highlight the changes in protein and AA metabolism occurring during perinatal and post-weaning exposure to a high-fat high-sugar diet, as well as those associated with individual differences in sensitivity to the induction of a MS by the same kind of diet. These alterations are tissuespecific and differ according to whether they result solely from differences in individual sensitivity to diet or whether they are also attributable to differences in the carbohydrate/lipid balance of the diet. Altogether, our results show that the development of MS is associated with changes in AA metabolic partitioning between the anabolic and oxidative pathways, differently affecting the liver, muscle, intestine and adipose tissue, and with an altered metabolic flexibility in muscle. This work opens the way to human studies, based on the measurements of δ15N and δ 13C in accessible pools.

Detailed chemical abundances of globular clusters in the Milky Way and M31 (the Andromeda Galaxy) are presented based on analyses of high resolution spectra. The unusual Milky Way cluster Palomar 1 (Pal 1) is studied through spectra of individual red giant branch stars; these abundances show that Pal 1 is not a classical globular cluster, and may have been accreted from a dwarf satellite of the Milky Way. The Milky Way globular clusters 47 Tuc, M3, M13, NGC 7006, and M15 are studied through their integrated light (i.e. a single spectrum is obtained for each cluster) in order to test high resolution integrated light analyses. The integrated abundances from these clusters reproduce the average abundances from individual stellar analyses for elements that do not vary within a cluster (e.g. Fe, Ca, and Ni). For elements that do vary within the clusters (e.g. Na and Mg) the integrated abundances fall within the observed ranges from individual stars. Certain abundance ratios are found to be extremely sensitive to uncertainties in the underlying stellar populations, such as input models, empirical relations to determine atmospheric parameters, interloping field stars, etc., while others (such as [Ca I/Fe I]) are largely insensitive to these effects. With these constraints on the accuracy and precision of high resolution integrated light analyses, detailed abundances are obtained for seven clusters in the outer halo of M31 that were recently discovered in the Pan-Andromeda Archaeological Survey (PAndAS) and are likely to have originated in dwarf galaxy satellites. Three clusters are relatively metal rich ([Fe/H] > −1.5) for their locations in the outer halo; their chemical abundances suggest that they likely originated in one or more fairly massive dwarf satellities. The other four are more metal-poor, and may have originated in less massive dwarf satellites. These results indicate that the Milky Way and M31 have both experienced some amount of accretion from dwarf satellites, though M31 may have had a more active accretion history. / Graduate

Alors que les galaxies évoluent, leur milieu interstellaire (MIS) s’enrichit continuellement en métaux, et cet enrichissement influence la formation d’étoiles. Les galaxies naines de faible métallicité de l’Univers Local sont les candidates idéales pour étudier l’influence de cet enrichissement en métaux sur les propriétés du MIS des galaxies et nous donne un aperçu des processus d’enrichissement et de formation stellaire dans des conditions proches de celles trouvées dans les systèmes pauvres en métaux de l’Univers primordial. Des études précédentes ont montré que le MIS des galaxies naines pose un certain nombre d’énigmes en terme d’abondance des grains, de composition de la poussière et même des processus d’émission en infrarouge lointain (FIR). Cependant, ces études étaient limitées à la poussière chaude émettant à des longueurs d’onde plus courtes que 200 micromètres et étaient effectuées sur un petit nombre de galaxies. Grâce à une sensibilité et une résolution améliorées dans les domaines FIR et submillimétriques (submm), Herschel nous donne une vue nouvelle sur les propriétés de la poussière froide dans les galaxies et nous permet d’étudier les galaxies les plus pauvres en métaux de manière systématique. Dans ce travail, je mène une étude des propriétés des poussières dans les galaxies naines et compare avec des environnements plus riches en métaux, pour aborder la question de l’impact de la métallicité sur les propriétés de la poussière. La nouveauté de ce travail réside dans le fait que les galaxies naines sont étudiées de manière systématique, nous permettant d’accéder aux, et de quantifier les propriétés générales représentatives de ces systèmes. Cette étude est conduite sur toute la gamme de longueurs d’onde infrarouge (IR)-submm, avec les nouvelles observations en FIR/submm d’Herschel, ainsi que des données Spitzer, WISE, IRAS, et 2MASS. Nous complétons ces données avec des mesures en domaine submm de télescopes au sol comme APEX ou le JCMT, pour étudier la présence et les caractéristiques de l’excès submm dans mon échantillon de galaxies. Je collecte aussi les données HI et CO pour accéder aux propriétés du gaz dans ces galaxies et étudier l’évolution du rapport en masse gaz-sur-poussière (G/D) avec la métallicité. Notre étude révèle des propriétés de poussière différentes dans les environnements de faible métallicité que celles observées dans des systèmes plus riches en métaux (par exemple, une poussière globalement plus chaude). Une émission en excès par rapport aux modèles utilisés, apparait souvent aux alentours de 500 micromètres, menant à d’importantes incertitudes sur les propriétés de la poussière, notamment sur la masse de poussière. Les excès les moins importants peuvent cependant être expliqués en utilisant une autre composition pour la poussière, avec des grains plus émissifs. Traceur idéal de l’état d’évolution chimique d’une galaxie, le G/D est en fait bien plus grand que ce que l’on pourrait attendre si l’on considère un modèle simple d’évolution chimique. Interprétée avec des modèles d’évolution chimique plus complexes, incorporant des processus de croissance des grains et/ou une formation d’étoiles épisodique, la relation entre le G/D et la métallicité, ainsi que sa dispersion, peuvent être expliquées par la grande variété d’environnements que nous considérons dans notre étude. / As galaxies evolve, their Interstellar Medium (ISM) becomes continually enriched with metals, and this metal enrichment influences the subsequent star formation. Low metallicity dwarf galaxies of the local Universe are ideal candidates to study the influence of metal enrichment on the ISM properties of galaxies and gives us insight into the enrichment process and star formation under ISM conditions that may provide clues to conditions in early universe metal-poor systems. Previous studies have shown that the ISM of dwarf galaxies poses a number of interesting puzzles in terms of the abundance of dust grains, the dust composition and even the FIR emission processes. However these studies were limited to the warmer dust emitting at wavelengths shorter than 200 microns and were done only on a small number of dwarf galaxies. Thanks to its increased sensitivity and resolution in FIR and submillimeter (submm) wavelengths, Herschel gives us a new view on the cold dust properties in galaxies and enables us to study the lowest metallicity galaxies in a systematic way. In this work, I carry out a study of the dust properties in dwarf galaxies and compare with more metal rich environments, in order to address the question of the impact of metallicity on the dust properties. The novelty of this work lays in the fact that dwarf galaxies are studied here in a systematic way, enabling us to derive and quantify the general properties that are representative of these systems. This study is conducted over the full IR-to-submm range, using new FIR/submm Herschel observations, Spitzer, WISE, IRAS and 2MASS data. We complete this set of data with longer submm measurements from ground-based facilities such as APEX and JCMT to study the presence and characteristics of the submm excess in my sample of galaxies. I also collect Hi and CO data to access the gas properties of the galaxies and study the evolution of the G/D with metallicity. Our study reveal different dust properties in low-metallicity environments than that observed in more metal-richs systems (e.g., an overall warmer dust component). An excess submm emission is often apparent near and/or beyond 500 microns rendering large uncertainties in the dust properties, even for something as fundamental as dust masses. Some of the smallest excesses can be explained by using another dust composition with more emissive grains. Ideal tracer of the chemical evolutionary stage of a galaxy, the gas-to-dust mass ratios (G/D) is found to be much higher than what is expected by simple chemical evolution models. Interpreted with more sophisticated chemical evolution models, including dust growth in the ISM and/or episodic star formation, the relation of the G/D with metallicity and its scatter can be explained by the wide variety of environments we are considering.

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L'archéologie galactique consiste à disséquer et analyser les nombreuses composantes de la Voie Lactée afin de mettre en évidence et distinguer les processus physiques qui contribuent à sa formation et son évolution. Ceci est possible grâce à une estimation précise des positions, des vitesses ainsi que des propriétés de l'atmosphère stellaire des étoiles individuelles qui appartiennent aux différents populations stellaires qui composent chacune de ces composantes. De ce fait, ce domaine dépend non seulement d'observations photométriques, astrométriques et spectroscopiques permettant de mesurer en détail les propriétés stellaires mentionnées mais également de modèles théoriques précis afin de les confronter avec les données observationnelles. Au cours de cette thèse, j'ai mené une étude détaillée sur les effets de fonction de sélection sur les abondances métalliques en utilisant des sondages spectroscopiques aux grandes échelles, suivi d'observations spectroscopiques de petites et grandes résolutions sur les parties internes de la Voie Lactée afin de caractériser la nature chimique du bulbe galactique ainsi que le taux de formation stellaire dans la zone centrale moléculaire (CMZ). Avec les présents et futurs grands sondages dédiés à l'archéologie galactique tels que APOGEE, RAVE, LAMOST, GALAH, etc.., il est essentiel de connaître la fonction de sélection spécifique qui est associée à la stratégie de ciblage de chacun de ces sondages. En utilisant des champs communs et des lignes de visée similaires entre APOGEE, LAMOST, GES et RAVE, et tout en considérant des modèles de synthèse de population stellaire, J'ai étudié les effets de fonction de sélection sur la fonction de distribution de la métallicité (MDF) et sur le gradient vertical de métallicité dans le voisinage solaire. Mes résultats indiquent qu'il y a un négligeable effet de fonction de sélection sur la MDF ainsi que sur le gradient vertical de métallicité. Ces résultats suggèrent alors que différents sondages spectroscopiques (de différentes résolutions et de longueurs d'onde) peuvent être combinés dans des études similaires à condition que les métallicité soient placées sur la même échelle. Tandis que de plus en plus d'observations spectroscopiques des régions externes du bulbe de la Voie Lactée révèlent la complexité de sa morphologie, sa cinétique et de sa nature chimique, les études détaillées sur les abondances chimiques de la région interne du bulbe (400-500 pc) font en revanche défaut. Je présenterai alors des spectres de haute résolution dans la bande K d'étoiles géantes K/M issues de cette région obscure et obtenus à partir du spectrographe de haute résolution dans l'infrarouge, CRIRES (R-50,000) situé au VLT. Je discuterai ensuite la MDF et les abondances chimiques détaillées de notre échantillon dans cette région et également la symétrie Nord-Sud dans la MDF le long du petit axe du bulbe. Un enjeu majeur dans les modèles d'évolution chimique est le manque de connaissance vis à vis de l'histoire et du taux de la formation stellaire de la Voie Lactée. La partie centrale de la Voie Lactée (<200 pc), appelée communément la zone centrale moléculaire, possède un grand réservoir de gaz moléculaire avec des indications d'activités de formation stellaire durant les 100 000 dernières années. J'ai utilisé des spectres KMOS (VLT) de petite résolution afin d'identifier et analyser les objects stellaires jeunes et massifs (YSOs) et afin d'estimer le taux de formation stellaire dans la CMZ en utilisant la méthode de contage YSO. / Galactic archaeology deals with dissecting the Milky Way into its various components with the objective to disentangle processes contributing to the Milky Way formation and evolution. This relies on precise estimation of positions, velocities as well as stellar atmosphere properties of individual stars belonging to different stellar populations that make up each of these components. Thus this field relies on photometric, astrometric and spectroscopic observations to measure the above mentioned stellar properties in detail in addition to accurate models to compare the observed results with. In this thesis, I have carried out a detailed study of selection function effects on metallicity trends using larges scale spectroscopic surveys, followed by high and low resolution spectroscopic observations towards the inner Milky Way to characterise the chemical nature of the inner Galactic bulge and to measure the star formation rate in the central molecular zone (CMZ), respectively. With ongoing and upcoming large Galactic archaeology spectroscopic surveys such as APOGEE, RAVE, LAMOST, GALAH etc, it is essential to know the specific selection function which is related to the targeting strategy of each of them. By using common fields along similar lines of sight between APOGEE, LAMOST, GES and RAVE, and together with stellar population synthesis models, I investigate the selection function effect on the metallicity distribution function (MDF) and the vertical metallicitiy gradients in the solar neighborhood. My results indicate that there is negligible selection function effect on the MDF and the vertical metallicity gradients. These results suggest that different spectroscopic surveys (different resolutions and wavelength range) can be combined for such studies provided their metallicities are put on the same scale. While more and more spectroscopic observations of the outer bulge regions reveal the complex morphological, kinematic and chemical nature of the Milky Way bulge, there is a lack of detailed chemical abundances studies in the inner bulge region (400-500 pc). I will present high resolution K-band spectra of K/M giants in this highly obscured region obtained using the high resolution infrared spectrograph, CRIRES (R-50,000), on VLT. I will discuss the MDF and detailed chemical abundances of our sample in this region as well as the North-South symmetry in MDF along the bulge minor axis. A major challenge in the chemical evolution models is the lack of knowledge about the star formation history and the star formation rate in the Milky Way. The inner 200 pc of the Milky way, the so called central molecular zone, has a large reservoir of molecular gas with the evidence of star formation activity during the last 100,000 years. I used low resolution KMOS spectra (VLT) to identify and analyse massive young stellar objects (YSOs) and estimated the star formation rate in the CMZ using the YSO counting method.

Globular clusters are tracers of the formation and evolution of their host galaxies. Kinematics, chemical abundances, age and position of the clusters allows tracing interactions between Milky Way and surrounding galaxies and outlines their chemical enrichment history. In this thesis we analyse mid-resolution spectra of about 800 red giant stars in 51 Galactic globular clusters. It is the first time that [Fe/H] and [Mg/Fe] derived in a consistent way are published for such a huge sample of globular clusters, almost 1/3 of the total number of catalogued clusters. Our metallicities are showed to be more precise than previous works based on mid-resolution spectroscopy. A turnover at [Fe/H] ~ -1.0 is found in the plot [Fe/H] vs. [Mg/Fe] for bulge and halo, although bulge seems to have a more metal-rich turnover, i.e, bulge has more efficient formation than the halo. Comparing the abundances with age the timescale for SNIa to start to become important is 1Gyr. [Fe/H] vs. age corroborates the different star formation efficiency of bulge and halo while [Mg/Fe] does not follow that. Halo was formed in mini halos or dwarf galaxies, and two multiple population clusters had their origin analysed to check it. M 22 seems to have been formed in the Milky Way while NGC 5824 possibly was originated in a dwarf galaxy, although our results are inconclusive for NGC 5824. The Galactic bulge seems to have been formed fast i.e., probably the oldest globular cluster is there. In fact HP 1 has a bluer horizontal branch than expected for its metallicity and we interpret that as an age effect. We determine its distance using light curves of variable stars in order to constrain future age determinations via colour-magnitude diagram. Finally, we investigate interaction between Milky Way and its neighbour galaxy SMC. We find that some star clusters are being stripped out of the SMC main body, which is consistent with tidal stripping scenario for the interaction between the galaxies, instead of ram pressure that would only affect gas. / Aglomerados globulares são traçadores da formação e evolução de suas galáxias. Cinemática, abundâncias químicas, idades e posições dos aglomerados permitem traçar interações entre Via Láctea e galáxias vizinhas e suas histórias de enriquecimento químico. Nesta tese analisamos espectros de média resolução de mais de 800 estrelas gigantes vermelhas em 51 aglomerados globulares Galácticos. É a primeira vez que [Fe/H] and [Mg/Fe] determinados de modo consistente são publicados para uma amostra desse porte, ~1/3 dos objetos catalogados. Nossas metalicidades são mais precisas que trabalhos anteriores similares. Uma quebra em [Fe/H] ~ -1.0 é encontrada no gráfico [Fe/H] vs. [Mg/Fe] para o bojo e halo, embora bojo parece ter uma quebra em [Fe/H] maior, i.e, bojo tem formaçãao mais eficiente que o halo. Comparando abundâncias com idade, a escala de tempo para SNIa ficar importante é 1Gano. [Fe/H] vs. idade corrobora diferentes eficiências de formação do bojo e halo, mas [Mg/Fe] vs. idade não mostra isso. O halo foi formado em mini halos ou galáxias anãs, e dois aglomerados com dispersão em [Fe/H] tiveram suas origens analisadas. M 22 parece ter sido formado na Via Láctea e NGC 5824 possivelmente foi originado em uma galáxia anã, embora os resultados são inconclusivos para NGC 5824. O bojo parece ter sido formado rapidamente e deve possuir o aglomerado mais velho. De fato, HP 1 tem um ramo horizontal mais azul que o esperado para sua metalicidade e vemos isso como um efeito da idade. Determinamos sua distância usando curvas de luz de RR Lyrae de maneira a restringir futuras determinações de idade via diagrama cor-magnitude. Finalmente, investigamos a interação entre Via Láctea e sua galáxia vizinha SMC. Encontramos aglomerados sendo removidos do corpo central da SMC, consistente com cenário de remoção por força de maré para a interação entre as galáxias, em vez de ``ram pressure\'\' que afeta só gás.

Globular clusters are tracers of the formation and evolution of their host galaxies. Kinematics, chemical abundances, age and position of the clusters allows tracing interactions between Milky Way and surrounding galaxies and outlines their chemical enrichment history. In this thesis we analyse mid-resolution spectra of about 800 red giant stars in 51 Galactic globular clusters. It is the first time that [Fe/H] and [Mg/Fe] derived in a consistent way are published for such a huge sample of globular clusters, almost 1/3 of the total number of catalogued clusters. Our metallicities are showed to be more precise than previous works based on mid-resolution spectroscopy. A turnover at [Fe/H] ~ -1.0 is found in the plot [Fe/H] vs. [Mg/Fe] for bulge and halo, although bulge seems to have a more metal-rich turnover, i.e, bulge has more efficient formation than the halo. Comparing the abundances with age the timescale for SNIa to start to become important is 1Gyr. [Fe/H] vs. age corroborates the different star formation efficiency of bulge and halo while [Mg/Fe] does not follow that. Halo was formed in mini halos or dwarf galaxies, and two multiple population clusters had their origin analysed to check it. M 22 seems to have been formed in the Milky Way while NGC 5824 possibly was originated in a dwarf galaxy, although our results are inconclusive for NGC 5824. The Galactic bulge seems to have been formed fast i.e., probably the oldest globular cluster is there. In fact HP 1 has a bluer horizontal branch than expected for its metallicity and we interpret that as an age effect. We determine its distance using light curves of variable stars in order to constrain future age determinations via colour-magnitude diagram. Finally, we investigate interaction between Milky Way and its neighbour galaxy SMC. We find that some star clusters are being stripped out of the SMC main body, which is consistent with tidal stripping scenario for the interaction between the galaxies, instead of ram pressure that would only affect gas. / Aglomerados globulares são traçadores da formação e evolução de suas galáxias. Cinemática, abundâncias químicas, idades e posições dos aglomerados permitem traçar interações entre Via Láctea e galáxias vizinhas e suas histórias de enriquecimento químico. Nesta tese analisamos espectros de média resolução de mais de 800 estrelas gigantes vermelhas em 51 aglomerados globulares Galácticos. É a primeira vez que [Fe/H] and [Mg/Fe] determinados de modo consistente são publicados para uma amostra desse porte, ~1/3 dos objetos catalogados. Nossas metalicidades são mais precisas que trabalhos anteriores similares. Uma quebra em [Fe/H] ~ -1.0 é encontrada no gráfico [Fe/H] vs. [Mg/Fe] para o bojo e halo, embora bojo parece ter uma quebra em [Fe/H] maior, i.e, bojo tem formaçãao mais eficiente que o halo. Comparando abundâncias com idade, a escala de tempo para SNIa ficar importante é 1Gano. [Fe/H] vs. idade corrobora diferentes eficiências de formação do bojo e halo, mas [Mg/Fe] vs. idade não mostra isso. O halo foi formado em mini halos ou galáxias anãs, e dois aglomerados com dispersão em [Fe/H] tiveram suas origens analisadas. M 22 parece ter sido formado na Via Láctea e NGC 5824 possivelmente foi originado em uma galáxia anã, embora os resultados são inconclusivos para NGC 5824. O bojo parece ter sido formado rapidamente e deve possuir o aglomerado mais velho. De fato, HP 1 tem um ramo horizontal mais azul que o esperado para sua metalicidade e vemos isso como um efeito da idade. Determinamos sua distância usando curvas de luz de RR Lyrae de maneira a restringir futuras determinações de idade via diagrama cor-magnitude. Finalmente, investigamos a interação entre Via Láctea e sua galáxia vizinha SMC. Encontramos aglomerados sendo removidos do corpo central da SMC, consistente com cenário de remoção por força de maré para a interação entre as galáxias, em vez de ``ram pressure\'\' que afeta só gás.