Made available in DSpace on 2015-03-26T13:45:35Z (GMT). No. of bitstreams: 1 texto completo.pdf: 1898009 bytes, checksum: 7ee3890dce9bee7dd59b31ebd52ed3a0 (MD5) Previous issue date: 2012-07-16 / Fundação de Amparo a Pesquisa do Estado de Minas Gerais / In this work, we study the graphs as invariants of stable Gauss maps from closed surfaces embedded in R3. We study the problem of realization of graphs by stable Gauss maps, emphasizing also cusp number of these maps. / Neste trabalho, estudamos os grafos como invariantes de aplicações de Gauss estáveis de superfícies fechadas mergulhadas em R3. Abordamos o problema de realização de grafos por aplicações de Gauss estáveis, enfatizando também o número de cúspides destas aplicações.

Thesis submitted in partial fulfilment of the requirements for the degree Doctor of Technology: Biomedical Technology In the Faculty of Health and Wellness Sciences At the Cape Peninsula University of Technology 2014 / Peroxidases are ubiquitous catalysts that oxidise a wide variety of organic and inorganic compounds employing peroxide as the electron acceptor. They are an important class of oxidative enzymes which are found in nature, where they perform diverse physiological functions. Apart from the white rot fungi, actinomycetes are the only other known source of extracellular peroxidases. In this study, the production of extracellular peroxidase in wild type actinomycete strains was investigated, for the purpose of large-scale production and finding suitable applications. The adjustment of environmental parameters (medium components, pH, temperature and inducers) to optimise extracellular peroxidase production in five different strains was carried out. Five Streptomyces strains isolated from various natural habitats were initially selected for optimisation of their peroxidase production. Streptomyces sp. strain BSII#1 and Streptomyces sp. strain GSIII#1 exhibited the highest peroxidase activities (1.30±0.04 U ml-1 and 0.757±0.01 U ml-1, respectively) in a complex production medium at 37°C and pH 8.0 in both cases. Maximum enzyme production for Streptomyces strain BSII#1 was obtained in the presence of 0.1 mM veratryl alcohol or pyrogallol, while 0.1 mM guaiacol induced the highest peroxidase production in Streptomyces sp. strain GSIII#1. As the highest peroxidase producer, Streptomyces sp. strain BSII#1 was selected for further studies. The strain was first characterised by a polyphasic approach, and was shown to belong to the genus Streptomyces using various chemotaxonomic, genotypic and phenotypic tests. Production of peroxidase was scaled up to larger volumes in different bioreactor formats. The airlift configuration was optimal for peroxidase production, with Streptomyces sp. strain BSII#1 achieving maximum production (4.76±0.46 U ml-1) in the 3 l culture volume within 60 hrs of incubation. A protocol for the purification of the peroxidase was developed, which involved sequential steps of acid and acetone precipitation, as well as ultrafiltration. A purification factor of at least 46-fold was achieved using this method and the protein was further analysed by LC-MS. The protein was shown to be a 46 kDa protein, and further biochemical characterisation showed that the peroxidase had a narrower spectrum of substrates as compared to reports on other peroxidases derived from actinomycetes. With 2,4-dichlorophenol as the substrate, the Km and Vmax for this enzyme were 0.893 mM and 1.081 μmol min-1, respectively. The purified peroxidase was also capable of catalysing coupling reactions between several phenolic monomer pairs. Overall, the peroxidase from Streptomyces sp. strain BSII#1 could feasibly be produced in larger scales and there remains further room to investigate other potential applications for this enzyme.

Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2016-09-09T17:41:52Z No. of bitstreams: 2 Dissertação - Lais Nogueira Magno - 2013.pdf: 3509435 bytes, checksum: a8540b89e1bed1cd2c787dc11faa9570 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-09-12T14:16:46Z (GMT) No. of bitstreams: 2 Dissertação - Lais Nogueira Magno - 2013.pdf: 3509435 bytes, checksum: a8540b89e1bed1cd2c787dc11faa9570 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2016-09-12T14:16:46Z (GMT). No. of bitstreams: 2 Dissertação - Lais Nogueira Magno - 2013.pdf: 3509435 bytes, checksum: a8540b89e1bed1cd2c787dc11faa9570 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2013-10-11 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / In the last years the seringueira latex has been received great attention from scientific community, particularly for its biomedical application, which can promote tissue regeneration, drug delivery, and others. However, based on some reports concerning allergic reaction, there has been increasing attention to search similar biomaterials with lower allergenic potential. Therefore, the present work, evaluated the potential of manganeira latex aiming a biomedical and technological applications. Biomembranes were prepared in a controlled manner, and were evaluated for their physical-chemistry characterization (FTIR, CHN, and TG) and its potential as a host matrix for photoluminescent compounds. Furthermore, bioassays were carried out of cell viability and genotoxicity. The results show that mangabeira biomembranes ares structurally similar to seringueira's and also show potential for use in biomedical and technological areas. / Nos últimos anos o látex de seringueira tem recebido grande atenção da comunidade científica, particularmente por suas aplicações biomédicas, as quais podem promover regeneração de tecidos, liberação de fármacos, dentre outros. Entretanto, baseado em alguns relatos sobre reações alérgicas, tem-se despertado interesse pela busca de biomateriais similares com menor potencial alergênico. Desta forma, no presente trabalho, foram avaliadas as potencialidades do látex de mangabeira visando aplicações tanto biomédicas quanto tecnológicas. Foram elaboradas biomembranas de forma controlada, e avaliados sua caracterização físico-química (FTIR, CHN e TG) e seu potencial como matriz hospedeira para compostos fotoluminescente. Além disso, foram realizados ensaios biológicos de viabilidade celular e genotoxicidade. Os resultados mostram que biomembranas de mangabeira são estruturalmente similares às de seringueira e com potencial para uso em áreas biomédicas e tecnológicas

Este trabalho tem por objetivo propor alguns critérios básicos, para o desenvolvimento de plásticos de engenharia em aplicações, componentes e partes dos veículos chamados populares, particularmente no Brasil, em substituição a partes tradicionalmente usinadas, fundidas ou injetadas em ligas metálicas. A despeito da desconfiança que cerca alguns dos componentes em plásticos que se destinam às aplicações automotivas, (e uma das principais razões para isso, são as elevadas temperaturas presentes em algumas áreas do automóvel), as grandes indústrias do setor não deixam de conceber novos projetos em polímeros de alta performance, para aplicações tidas como críticas em termos de resistência geral. Os chamados plásticos de engenharia são preferivelmente escolhidos devido à sua fácil processabilidade, e sua flexibilidade em propiciar desenhos mais complexos, com boa estabilidade dimensional e uma excelente resistência à corrosão em ambientes de hostilidade química. No passado, as empresas claramente optavam pelos plásticos chamados de termofixos, que hoje estão quase esquecidos ou obsoletos (principalmente devido aos processamentos mais custosos e/ou mais lentos). Hoje são largamente substituídos pelos termoplásticos de engenharia. Atualmente, o consumo de plásticos em alguns carros brasileiros já alcança quase 130 kg de plásticos nos seus interiores e/ou exteriores (como o Ford KA, por exemplo), o que ajuda a reduzir o peso total do automóvel, aumentando a economia de combustível, reduzindo os custos de processos, aliada a uma excelente aparência superficial. Este trabalho versará sobre a possibilidade de se propor critérios mais objetivos na fase inicial de um processo de seleção de plásticos de engenharia (notadamente, poliamidas, acetais, poliésteres, e policarbonato), aumentando a eficácia da escolha e diminuindo custos (evitando erros e economizando tempo), como um guia prático de consulta para engenheiros e profissionais do setor automotivo brasileiro. / The main objective of this paper is to propose some basic economical and objective criteria for the engineering plastics development in applications, components and parts in the low-end cars segment (known in Brazil as popular cars), to replace traditional metallic machined, die-casted or injected parts. In spite of the lack of reliance in plastics components in automotive applications (one of them being the high temperature in the engine area), big industries in the sector always conceive new projects on high performance polymers for applications considered critical because of its general resistance. The named engineering plastics are preferably chosen due to its easy processing, complex design flexibility, with a good dimensional stability and an excellent resistance against corrosion in environments with chemical hostility. In the past, companies clearly selected the called thermo-stable plastics, almost forgotten or obsolete nowadays (mainly due to the high cost and/or slowness on processing). Today they are largely replaced by engineering thermoplastics. Nowadays, the consumption of plastics in Brazilian low-end cars already reached approximately 130 kg of plastics in their interior and/or exterior (like in Ford KA, for example), which helps to reduce total car weight, increasing the saving of fuel, mitigating process costs, besides to an excellent surface appearance. This paper will approach about the possibility of proposing more objective criteria in the initial phase of the engineering plastics selection (notably polyamides, acetals, polyesters, and polycarbonates), increasing the choice efficacy and diminishing costs (avoiding mistakes and saving time), as a practical consulting guide to engineers and professionals from the Brazilian automotive sector.

Les systèmes embarqués sont de plus en plus complexes et requièrent des besoins en puissance de calcul toujours plus importants. Ils doivent être capables de s'adapter à l'évolution rapide de leurs applications qui requièrent un haut niveau de performance (ordre du TOPS: Téra-opérations par seconde) et de parallélisme. Par ailleurs, la complexité des parties irrégulières étant de plus en plus importantes, des solutions de calcul performantes et adaptées doivent être mises en œuvre afin de prendre en compte leur dynamisme. Une prise en compte efficace du dynamisme réduit le déséquilibre de charge entre les ressources de calcul et améliore grandement les performances globales.Pour répondre aux besoins de ces applications de calcul intensif massivement parallèle et dynamique, nous proposons dans cette thèse l’architecture AHDAM qui signifie « Asymmetric Homogeneous with Dynamic Allocator Manycore architecture ». Cette architecture a été conçue afin de masquer efficacement la latence d’accès à la mémoire extérieure dont de nombreux accès sont nécessaires lors de la manipulation de grands volumes de données. Pour cela, des processeurs multitâches ont été utilisés. Par ailleurs, l’architecture AHDAM imbrique plusieurs niveaux de parallélisme afin de tirer partie efficacement des différentes formes de parallélisme des applications, et ainsi atteindre un haut niveau de performance. Enfin, cette architecture utilise un contrôleur centralisé pour équilibrer la charge de calcul entre ses ressources de calcul afin d’augmenter leur taux d’utilisation et supporter les applications fortement dynamiques.L’architecture AHDAM a été évaluée en portant une application de radio logicielle appelée «spectrum radio-sensing ». Avec 136 cœurs cadencés à 500 MHz, l'architecture AHDAM atteint une performance crête de 196 GOPS et répond aux exigences de l'application. / Embedded systems are getting more complex and require more intensive processing capabilities. They must be able to adapt to the rapid evolution of the high-end embedded applications that are characterized by their high computation-intensive workloads (order of TOPS: Tera Operations Per Second), and their high level of parallelism. Moreover, since the dynamism of the applications is becoming more significant, powerful computing solutions should be designed accordingly. By exploiting efficiently the dynamism, the load will be balanced between the computing resources, which will improve greatly the overall performance. To tackle the challenges of these future high-end massively-parallel dynamic embedded applications, we have designed the AHDAM architecture, which stands for “Asymmetric Homogeneous with Dynamic Allocator Manycore architecture". Its architecture permits to process applications with large data sets by efficiently hiding the processors' stall time using multithreaded processors. Besides, it exploits the parallelism of the applications at multiple levels so that they would be accelerated efficiently on dedicated resources, hence improving efficiently the overall performance. AHDAM architecture tackles the dynamism of these applications by dynamically balancing the load between its computing resources using a central controller to increase their utilization rate.The AHDAM architecture has been evaluated using a relevant embedded application from the telecommunication domain called “spectrum radio-sensing”. With 136 cores running at 500 MHz, AHDAM architecture reaches a peak performance of 196 GOPS and meets the computation requirements of the application.

Le travail de recherche consiste à développer des hydrogels pour la prolifération et la migration cellulaires in vitro et in vivo en trois dimensions (3D). Des hydrogels à base de polyamidoamines avec d'intéressantes propriétés physicochimiques et une remarquable biocompatibilité ont été développés pour différentes applications biomédicales. Un hydrogel avec des sondes luminescentes d’iridium(III) incorporés de manière covalente, a été conçue comme plate-forme 3D de culture cellulaire, pour la visualisation directe des cellules vivantes en temps réel, et a démontré être un puissant outil de bioimagerie in vitro. En outre, un hydrogel nanocomposite, capable d'induire la chimiotaxie des cellules souches, a été développé et testé in vivo, en confirmant son potentiel en tant qu’implant pour l’ingénierie tissulaire. Finalement, un hydrogel injectable et biodégradable a été réalisé comme un nouvel agent pour la dissection sous-muqueuse endoscopique des lésions néoplasiques digestives. / The research work focuses on the development of hydrogels to investigate three-dimensional (3D) cell proliferation and migration in vitro and in vivo. Polyamidoamines-based hydrogels with interesting physicochemical properties and high biocompatibility have been developed for different biomedical applications. An hydrogel with covalently incorporated iridium(III) fluorescent probes, has been conceived as a 3D cell culture platform for the direct visualization of living cells in real-time, demonstrating to be a powerful tool for in vitro bio-imaging. Moreover, a nanocomposite hydrogel, able to induce chemotaxis of stem cells, was developed andtested in vivo, confirming its potential as a tissue engineering implant. Finally, an injectable biodegradable nanocomposite hydrogel was realized as a novel agent for endoscopic submucosal dissection of large neoplastic lesions of the gastro-intestinal tract.

Le développement des polymères conducteurs, surtout en termes de stabilité, a permis de les intégrer dans les dispositifs électroniques pour des applications à haute valeur ajoutée. C'est la raison pour laquelle les condensateurs tantale initialement basés sur la technologie MnO2, en tant que cathode, ont été améliorés avec le remplacement de cette dernière par un polymère organique conducteur. Ces nouveaux condensateurs tantale-polymère sont constitués d'une anode en tantale frittée, d'un film diélectrique en oxyde de tantale, et d'une cathode en polymère conducteur, typiquement le poly(3,4-éthylènedioxythiophène) (PEDOT). Le fonctionnement des condensateurs a d'abord été optimisé uniquement pour de faibles capacités par polymérisation in situ. L'étape suivante consiste à atteindre de plus grandes capacités par imprégnation du polymère conducteur pré-synthétisé. Notre travail a été axé sur la caractérisation fine et la fabrication de prototypes de condensateur. Pour mener à bien cette étude, chaque partie du condensateur tantale-polymère a été caractérisée par différentes techniques physico-chimiques. Nous avons, entre autres, étudié la microstructure du réseau de tantale et les propriétés de la solution commerciale de polymère conducteur pour déterminer les paramètres d'imprégnation des condensateurs. Les caractérisations effectuées au laboratoire sont complétées par une évaluation des performances électriques des prototypes fabriqués dans l'entreprise. Tout ce travail a contribué à la mise sur le marché d'une nouvelle gamme de condensateurs tantale-polymère par l'entreprise Exxelia Tantalum. En parallèle, une étude a été consacrée à la synthèse d'un nouveau couple de polymère plus performant dans le but de remplacer le polymère commercial. / The development of conducting polymers, especially in terms of environmental stability, has allowed them to be used in electronic devices for high value applications. That's why tantalum capacitors initially based on MnO2 cathode technology have been improved by the replacement of it with a conducting polymer. Tantalum-polymer capacitors consist of a sintered tantalum anode, an anodic tantalum oxide film as a dielectric, and a conductive polymer cathode made of poly (3,4-ethylenedioxythiophene) (PEDOT). Until recently, those capacitors have been optimized only for low capacities by in situ polymerization. The next step is to reach higher capacities using an impregnated conductive polymer. Our work focused on the characterization and fabrication of capacitors. The main study focused on the characterization of each part of the tantalum-polymer capacitor via physico-chemical investigations. We studied the microstructure of the tantalum network and the properties of the commercial polymer solution to determine parameters for the dip-coating of tantalum anodes. This laboratory characterization is complemented by an assessment of the electrical performances of samples within the company. All this work has contributed to a new range of tantalum-polymer capacitors by Exxelia Tantalum Company. At the same time, a study has been performed in the synthesis of a new pair of polymers in order to replace the commercial polymer.

Thesis analyses the problematics of determining both qualitative and quantitative parameters for testing on projects involving development of mobile applications. First, content and scope of testing is fixed, followed by the specification of influence model. Methodology is proposed to ascertain the financial and temporal extent of the testing based on obtained influence model.

The aim of this thesis is the analysis of using web and mobile applications in team work within projects. Thesis is divided into the two main parts. Firstly, the theoretical part provides basic information and facts about work at a team, communication and team work on common projects. Theoretical part also includes a definition and development of web and mobile applications from past until now. Secondly, the practical part includes analysis by the survey. The survey was conducted using two different methods - questionnaire survey method and structured interviews. The questionnaire was focused on the usage of basic applications for communication, cooperation and coordination within work teams. The structured interviews was focused more on benefits for employees

Rephotography has been a popular research topic in the photography field for a long time. The purpose of rephotography itself is to repeatedly take photographs of the same scene at a different time. As a result, the sequence of rephotographs with the reference, often historical, the picture provides a compelling visualization of the evolution of the subject or capture its changes in time. However, the act of rephotography is difficult for the rephotographers as they have to cope with the ambiguous motions in six degrees of freedom and with the changes of the subject itself or its surrounding environment.        This thesis aims to create a mobile application that would help its users to capture a rephotograph more accurately and allow them to share the scenes amongst other users. The designed application uses available on-device sensors to navigate the user to the location and guide the user during the rephotography process to capture a precise rephotograph. Furthermore, the application contains user interface elements designed explicitly for rephotography. Moreover, the work describes topics about user interface design, iOS application development, and designing and deploying backend API for the mobile application.