Solid-state nanopores are a versatile class single-molecule sensors to electrically characterize a range of biological molecules. Nanopores operate on the simple premise that when a voltage is applied across a pore immersed in a salt solution, the passage of a biomolecule results in a transient blockage in the ionic current that provide information about the translocating molecule. This thesis presents studies employing various DNA nanostructures with solid-state nanopore electrical readout for the development of high sensitivity digital single-molecule assays to detect low-abundance biomarkers. Toward this ultimate goal, work presented in this thesis use nanopores to probe DNA nanostructures, their assembly, mechanical properties, and monitor their dynamics with time and temperature. DNA nanostructures are self-assembled via specific base pairing of DNA, their programmability make them particularly useful for applications including drug delivery, molecular computation and biosensing. Here, I first show results of translocation profiles and discuss folding characteristics, mobility, and molecular configuration during passage for different DNA nanostructures such as the short star-shaped DNA nanostructures and large helix-bundle DNA origami structures under various experimental conditions in an effort to understand the passage characteristics through nanopores of these structures before using them in biological assays. I conclude by presenting a magnetic bead-based immunoassay scheme using a digital solid-state nanopore readout. Nanopore has the ability to count molecules one at a time, this allows accurate and precise determination of the concentration of a biomarker in solution. Coupled with the use of specific choice of DNA nanostructures, as proxy labels for proteins of interest, I establish that nanopores sensors can reliably quantify the concentration of a protein biomarker from complex biofluids and overcome the traditional challenges associated with nanopore-based protein sensing, such as specificity, sensitivity, and consistency. I demonstrate the quantification of thyroid stimulating hormone (TSH) with a high degree of precision down to the femtomolar range by using a nanoparticle-based signal amplification strategy. The proposed assay scheme is generalizable to a framework for the detection and quantification of a wide range of target proteins, and given that its performance can further be improved with the use of parallelization, preconcentration, or miniaturization, it opens up exciting opportunities for the development of ultra-sensitive digital assay in a format that is compatible for point-of-care.

This dissertation explores CMOS RF design and reliability for portable wireless receivers. The objective behind this research is to achieve an increase in integration level, and gain more understanding for RF reliability. The fields covered include device, circuit and system. What is under investigation is a multi-band multi-mode receiver with GSM, DCS-1800 and CDMA compatibility. To my understanding, GSM and CDMA dual-mode mobile phones are progressively investigated in industries, and few commercial products are available. The receiver adopts direct conversion architecture. Some improved circuit design methods are proposed, for example, for low noise amplifier (LNA). Except for band filters, local oscillators, and analog-digital converters which are usually implemented by COTS SAW filters and ICs, all the remaining blocks such as switch, LNA, mixer, and local oscillator are designed in MOSIS TSMC 0.35[micro]m technology in one chip. Meanwhile, this work discusses related circuit reliability issues, which are gaining more and more attention. Breakdown (BD) and hot carrier (HC) effects are important issues in semiconductor industry. Soft-breakdown (SBD) and HC effects on device and RF performance has been reported. Hard-breakdown (HBD) effects on digital circuits have also been investigated. This work uniquely address HBD effects on the RF device and circuit performance, taking low noise amplifier and power amplifier as targets.

As CMOS device sizes shrink, the channel electric field becomes higher and the hot carrier (HC) effect becomes more significant. When the oxide is scaled down to less than 3 nm, gate oxide breakdown (BD) often takes place. As a result, oxide trapping and interface generation cause long term performance drift and related reliability problems in devices and circuits. The RF front-end circuits include low noise amplifier (LNA), local oscillator (LO) and mixer. It is desirable for a LNA to achieve high gain with low noise figure, a LO to generate low noise signal with sufficient output power, wide tuning range, and high stability, and a mixer to up-convert or down-convert the signal with good linearity. However, the RF front-end circuit performance is very sensitive to the variation of device parameters. The experimental results show that device performance is degraded significantly subject to HC stress and BD. Therefore, RF front-end performance is degraded by HC and BD effects. With scaling and increasing chip power dissipation, operating temperatures for device have also been increasing. Another reliability concern, which is the negative bias temperature instability (NBTI) caused by the interface traps under high temperature and negative gate voltage bias, arises when the operation temperature of devices increases. NBTI has received much attention in recent year and it is found that NIT is present for all stress conditions and NOT is found to occur at high VG. Therefore, the probability of BD in pMOSFET increases with temperature since trapped charges during the NBTI process increase, thus resulting in percolation, a main cause of oxide degradation. The above effects can cause significant degradations in transistors, thus leading to the shifts of RF performance. This dissertation focuses on the following aspects: (1) RF performance degradation in nMOSFET and pMOSFET due to hot carrier and soft breakdown effects are examined experimentally and will be used for circuit application in the future. (2) A modeling method to analyze the gate oxide breakdown effects on RF nMOSFET has been proposed. The device performance drifts due to gate oxide breakdown are examined, breakdown spot resistance and total gate capacitance are extracted before and after stress for 0.16 um CMOS technology. (3) LC voltage controlled oscillator (VCO) performance degradation due to gate oxide breakdown effect is evaluated. (4) NBTI, HCI and BD combined effects on RF performance degradation are investigated. A physical picture illustrating the NBTI induced BD process is presented. A model to evaluate the time-to-failure (TTF) during NBTI is developed. DCIV method is used to extract the densities of NIT and NOT. Measurements show that there is direct correlation between the steplike increase in the gate current and the oxide-trapped charge (NOT). However, Breakdown has nothing to do with interface traps (NIT). (5) It is found that the degradation due to NSH stress is more severe than that of NS stress at high temperature. A model aiming to evaluate the stress-induced degradation is also developed.

Laser-Induced Breakdown Spectroscopy (LIBS) and Raman spectroscopy are still growing analytical and sensing spectroscopic techniques. They significantly reduce the time and labor cost in analysis with simplified instrumentation, and lead to minimal or no sample damage. In this dissertation, fundamental studies to improve LIBS analytical performance were performed and its fusion with Raman into one single sensor was explored. On the fundamental side, Thomson scattering was reported for the first time to simultaneously measure the electron density and temperature of laser plasmas from a solid aluminum target at atmospheric pressure. Comparison between electron and excitation temperatures brought insights into the verification of local thermodynamic equilibrium condition in laser plasmas. To enhance LIBS emission, Microwave-Assisted LIBS (MA-LIBS) was developed and characterized. In MA-LIBS, a microwave field extends the emission lifetime of the plasma and stronger time integrated signal is obtained. Experimental results showed sensitivity improvement (more than 20-fold) and extension of the analytical range (down to a few tens of ppm) for the detection of copper traces in soil samples. Finally, laser spectroscopy systems that can perform both LIBS and Raman analysis were developed. Such systems provide two types of complimentary information – elemental composition from LIBS and structural information from Raman. Two novel approaches were reported for the first time for LIBS-Raman sensor fusion: (i) an Ultra-Violet system which combines Resonant Raman signal enhancement and high ablation efficiency from UV radiation, and (ii) a Ti:Sapphire laser based NIR system which reduces the fluorescence interference in Raman and takes advantage of femtosecond ablation for LIBS.

The aerodynamics of wings with moderately swept wings continues to be a challenging and important problem due to the current and future use in military aircraft. And yet, there is very little work devoted to the understanding of the aerodynamics of such wings. The problem is that such wings may be able to sustain attached flow next to broken-down delta-wing vortices, or stall like two-dimensional wings, while shedding vortices with generators parallel to their leading edge. To address this situation we studied the flow field over diamond-shaped planforms and sharp-edged finite wings. Possible mechanisms for flow control were identified and tested. We explored the aerodynamics of swept leading edges with no control. We presented velocity and vorticity distributions along planes normal and parallel to the free stream for wings with diamond shaped planform and sharp leading edges. We also presented pressure distributions over the suction side of the wing. Results indicated that in the inboard part of the wing, an attached vortex can be sustained, reminiscent of delta-wing type of a tip vortex, but further in the outboard region 2-D stall dominated even at 13° AOA and total stall at 21° AOA. To explore the unsteady flow field and the effectiveness of leading-edge control of the flow over a diamond-planform wing at 13° AOA, we employed Particle Image Velocimetry (PIV) at a Reynolds number of 43,000 in a water tunnel. Our results indicated that two-D-like vortices were periodically generated and shed. At the same time, an underline feature of the flow, a leading edge vortex was periodically activated, penetrating the separated flow, eventually emerging downstream of the trailing edge of the wing. To study the motion and its control at higher Reynolds numbers, namely 1.3 x 106 we conducted experiments in a wind tunnel. Three control mechanisms were employed, an oscillating mini-flap, a pulsed jet and spanwise continuous blowing. A finite wing with parallel leading and trailing edges and a rectangular tip was swept by 0°, 20°, and 40° and the pulsed jet employed as is control mechanism. A wing with a diamond-shaped-planform, with a leading edge sweep of 42°, was tested with the mini-flap. Surface pressure distributions were obtained and the control flow results were contrasted with the no-control cases. Our results indicated flow control was very effective at 20° sweep, but less so at 40° or 42°. It was found that steady spanwise blowing is much more effective at the higher sweep angle. / Ph. D.

Laser-induced breakdown spectroscopy (LIBS) is a technique that allows quantitative and qualitative analysis of many materials. In this study, the LIBS analysis options for strontium mixture powders is expanded by increasing the number of usable strontium atomic transitions to avoid incorrect results due to spectral congestion or high strontium concentrations. The research employs double-sided tape affixed to a glass slide to hold the sample where the powder is poured onto one surface of the tape and excess dust that has not adhered is removed. This method minimizes the sample quantity needed and keeps the sample on the slide during experimentation, which also reduces costs. Herein, LIBS was used to detect and quantify the level of metal concentrations in used engine oil samples to provide valuable information about the composition of the selected material in a liquid sample. Data were obtained using multivariate analysis to develop calibration curves using LIBS spectra, which was employed for the quantification of the elements Al, Ca, Fe, Mg, and Mn. The relationship between the peak intensity of the metals in new engine oil samples and the metal concentrations in used engine oil samples were analyzed to minimize the matrix effect and the interference of element lines after which the atomic emission observed in LIBS spectra of used engine oil and new engine oil were compared. C2 molecular band emissions were also used to determine the degree of the engine oil degradation. Next, calibration models were developed for samples with high species concentrations. A partial least squares regression model was developed for calibration models to overcome matrix effect problems of some lines of each metal. This research successfully used the LIBS technique to determine the degree of engine oil degradation. This study established that used engine oil analysis using the LIBS technique can be utilized to maintain engines in good condition and to prevent engine failure. This paper presents the key findings and conclusions regarding the application of LIBS. Finally, although this technique shows many benefits and reliable results, challenges remain in terms of matrix effects, spectral pre-processing, model calibration, and instrumentation.

<p dir="ltr">This dissertation reports experiments and simulations of micro-/nanoscale electrical breakdown, connects them to the microscale breakdown theories, relates them to field emission and space-charge-limited conditions, and demonstrates the modification of the approach to microwave fields. It provides the first comprehensive experimental assessment of the transitions between electron emission and gas breakdown mechanisms at microscale and nanoscale and extension of semi-empirical laws for ionization process in DC and microwave. These findings will be valuable in developing theories to predict electron emission and gas breakdown mechanisms, which provides guidance for nanoscale device design.</p>

Significant progress in reducing the growth temperature of the SiC epitaxial growth became possible in the previous work by using new chloro-carbon epitaxial growth method. However, it was established that even in the new process, homogenous nucleation of Si in the gas phase limited the growth rate. In the present work, new chlorinated silicon precursor SiCl4 was investigated as a replacement for the traditional silicon precursor SiH4 during the low-temperature chlorocarbon epitaxial growth. The new process completely eliminated the homogenous nucleation in the gas phase. Growth rate of 5-6 μm/h was achieved at 1300°C compared to less than 3 μm/h in the SiH4-based growth. The growth dependence on the C/Si ratio revealed that the transition from the C-supply-limited to the Si-supply-limited growth mode takes place at the value of the C/Si ratio much higher than unity, suggesting that certain carbon-containing species are favorably excluded from the surface reactions in the new process. Morphology degradation mechanisms, which are unique for the lowtemperature growth, were observed outside the established process window. Prior to this work, it remained unclear if CH3Cl simply served as a source of Cl to suppress homogeneous nucleation in the gas phase, or if it brought some other unknown improvements. In this work true benefits of CH3Cl in providing unique improvement mechanisms have been revealed. It was established that CH3Cl provided a much wider process window compared to C3H8. In contrast, even a very significant supply of extra Cl from a chlorinated silicon precursor or from HCl during the C3H8-based growth could not provide a similar benefit. The combination of the chloro-carbon and the chloro-silane precursors was also investigated at conventional growth temperature. High-quality thick epitaxial layers, with the growth rate up to 100μm/h were obtained, and the factors influencing the growth rate and morphology were investigated. Extensive optical and electrical characterization of the low-temperature and the regular-temperature epitaxial layers was conducted. The device-quality of the lowtemperature chloro-carbon epilayers was validated for the first time since the development of the chloro-carbon epitaxial process in the year 2005 by fabricating simple Schottky diodes and investigating their electrical characteristics.

No description available.

No description available.