A technique involving Restriction Fragment Length Polymorphism (RFLP) was used to observe the DNA fragment polymorphism between a bean cultivar with I/I genotype and a bean cultivar with i/i genotype. The I gene encodes immunity to bean common mosaic virus (BCMV).

No description available.

Stage of maturity at harvest and relative seed size can affect seed vigor. Greenhouse and field studies were conducted to determine the effect of seed maturity on seed vigor, storability, and subsequent plant growth of four cultivars of snap bean (Topcrop, Provider Black, Provider White, and Cherokee Wax). Seeds harvested between physiological maturity (PM) and harvest maturity (HM) showed the highest seed vigor and storability. At PM, seed moisture content was about 55%. A drop in seed fresh weight and a pod color change from green to yellow appear to signal the stage of PM. Delaying harvest past HM reduced seed vigor. The three cultivars with colored seed coats showed higher seed vigor than Provider White. Climatic and weathering effects on maturity, vigor, and yield of Topcrop and Cherokee Wax seed were evaluated at three locations (L-I, L-2, and L-3) in Sri Lanka having different agro-climatic conditions. Seeds that developed and matured under cooler conditions (L-l) produced higher yields and had larger size, better color, and higher vigor. Seeds developed and matured faster under warmer conditions (L-3); however, seed vigor and yields were lower and seed vigor dropped prior to HM. Cherokee Wax was the most tolerant to field weathering. Seeds of Topcrop and Cherokee Wax were separated by weight into three seed sizes (small, medium, and large) and evaluated for crop performance in two plantings (12/90 and 3/91). Field emergence was higher from larger seeds in the second planting, where the soil conditions were more stressful. Seedlings and plants at the flowering stage were larger and pod yields higher from larger seeds. The cultivar x seed size interaction was significant for pod yield per plant. Topcrop showed no differences, while with Cherokee Wax, pod yield per plant increased with increase in seed size during both plantings. Seeds produced from small seeds were similar or higher in vigor, indicating that small seeds could be used for seed production purposes. The data from these experiments indicate that vigor in snap bean seeds can be optimized by harvesting at an early stage after PM and by grading to remove small seeds. / Ph. D.

Microwave Blanching instructions for a variety of vegetables have recently been published by several sources. More research is needed, however, to establish microwave blanching times that are sufficient to destroy enzymes which cause nutritional and sensory deterioration in frozen vegetables while maintaining optimal quality. In the present study the General Electric Company's recommended blanch times for carrots and green beans were investigated using three microwave ovens. Peroxidase was used as an indicator enzyme for measuring blanching adequacy. It was determined that oven variation had no significant effect on percent residual peroxidase activity in either vegetable (p > 0.05). A large degree of standard deviation in the percent residual peroxidase activity was found among the blanched portions of vegetable. This variation was attributed to the inconsistent levels of peroxidase enzyme in the vegetables themselves. Blanch time had the most significant impact on percent residual peroxidase activity (p < 0.01). After a four-minute blanch, carrots retained approximately 21-25% peroxidase activity and green beans retained approximately 37-46% peroxidase activity. These ranges are much higher than the acceptable level of 5% residual peroxidase activity. Six minutes of blanching for green beans and five minutes of blanching for carrots were required to reduce residual peroxidase activity to acceptable levels. Vegetables with such low peroxidase activity show no deleterious enzyme related changes during frozen storage. Microwave blanching has the potential to be an acceptable alternative to hot water blanching, but further research is needed to optimize microwave blanching procedures. / Master of Science

A problem solving environment (PSE) provides all computational facilities necessary for solving a target class of problems efficiently. PSEs are used primarily for domain-specific problem-solving in science and engineering and aim to ease the burden of advanced scientific computing. Scientific problem solving, however, often involves the use of legacy resources which are difficult to modify or port, and may be distributed on different machines. Existing PSEs provide little support for solving such problems in a generic framework. This thesis investigates the design of a platform-independent system that enables problem solving using legacy resources without having to modify legacy code. It presents Symphony, an open and extensible Java-based framework for composition and manipulation of distributed legacy resources. Symphony allows users to compose visually a collection of programs and data by specifying data-flow relationships among them and provides a client/server framework for transparently executing the composed application. Additionally, the framework is web-aware and helps integrate web-based resources with legacy resources. It also enables programmers to provide a graphical interface to legacy applications and to write visualization components. Symphony uses Sun Microsystems' JavaBeans component architecture for providing components that represent legacy resources. These components can be customized and composed in any standard JavaBeans builder tool. Executable components communicate with a server, implemented using Java Remote Method Invocation mechanism, for executing remote legacy applications. Symphony enables extensibility by providing abstract components which can be extended by implementing simple interfaces. Beans implemented from the abstract beans can act as data producers, consumers or filters. / Master of Science

Made available in DSpace on 2016-12-08T16:44:53Z (GMT). No. of bitstreams: 1 PGPV07MA017.pdf: 316351 bytes, checksum: 90f3d3dd654e7567ae2c313d163704f6 (MD5) Previous issue date: 2006-02-24 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The indications for the beginning of the application of fungicides to control the common bean diseases are scarce and few precise, following different criteria, as: growth stages, fixed program of calendar, information on climatic conditions, inoculum density and disease intensity in the plant.Most of these cases, the applications of fungicides are made in preventively, without disease occurrence. This fact has taken an irrational use of fungicides, with the increase of the cost of production and threat to the environment. The objective of this work was compared criteria for the decision of the fungicide application to control anthracnose, angular leaf spot and rust of the common bean.The experiments was carry out in a commercial field in Vacaria/RS, during the growing season of 2005, under no-till system and crop rotation, with plots the 3 x 5 m, in a randomized block design with four replications Three cultivars were evaluated, using two fungicides per cultivar: BRS Campeiro (triphenyltin hydroxide and azoxystrobin + cyproconazol), IPR Uirapuru (tiophanate methyl + chlorothalonil and tetraconazol) and BRS Valente (tryfloxystrobin + propiconazol and tebuconazol).The criteria for the fungicide application was: fixed calendar (FC), growth stage (GS) and threshold (LDE) of 10 % and 20 % of disease incidence. Weekly evaluation was carry out for disease incidence and severity of 20 trifoliolate leves at first trifoliolate leaf until physiological maturation. The values of disease incidence and severity were submitted to area under disease progress curve (AUDPC).The central plants of a square meter in each parcels were used to yield calculation and grain pathology. An economic analysis was carry through on the basis of the costs of the six fungicides, the kneading of three types of sprayer (mounted, towed and Uniport model) and the fixed and changeable costs of the tractor. The data of the AUDPC, yield, mass of thousand grains and seed´s pathology were submitted to analysis of variance and the averages compared by Tukey test at 5% of probability. In the FC and the GS criteria, three applications of fungicides were used, being the two first application with the fungicides to anthracnose and angular leaf spot disease and the last one to rust. For the LDE 10 % and LDE 20 % criteria it was only one application to control the rust disease. Due to seeds sanity by crop rotation and to the irregularity of pluvial precipitations, it was not detected anthracnose and angular leaf spot at none time of evaluation. Amongst the fungi diseases, the rust was detected only after the fourth evaluation. The data of the AUDPC for rust disease in the three cultivars was low. The cultivar IPR Uirapuru it was more susceptible to the rust disease, while the BRS Campeiro more tolerant. Significant differences was not detected in the grains yield and the mass of a thousand grains between treatments and cultivars. Colletotrichum lindemunthianum and Phaeisariopsis griseola was not detected in the grains, indicating the grains sanity was a consequence of plants health during the growth season. The yield of BRS Campeiro cultivar (67 kg or R$ 78,15), has not covered by the cost of the fungicide applications. Instead, in the IPU Uirapuru cultivar, with high economic production (124 kg or R$ 144,66), the cost of the fungicide applications to disease control it was covered by one application only in determinate cases, considering the types of fungicides e sprayers. It was concluded that preventive applications in F.C. and G.S. are unnecessary when the source of primary inoculum of anthracnose and angular leaf spot will be eliminated and the intensity the rust will be low / As indicações para o início da aplicação de fungicidas no feijoeiro comum são escassas e pouco precisas, seguindo diferentes critérios, como: estádios fenológicos, programa fixo de calendário, informações sobre condições climáticas, densidade de inóculo na área e intensidade da doença na planta. Na maioria destes casos as aplicações de fungicidas são feitas de modo preventivo, sem a ocorrência da doença. Este fato tem levado ao uso irracional de fungicidas, com o aumento do custo de produção e ameaça ao meio ambiente. O presente trabalho teve como objetivo comparar critérios para tomada de decisão de aplicações de fungicidas visando o controle da antracnose, mancha angular e ferrugem do feijoeiro comum. Os experimentos foram conduzidos em lavoura comercial, em Vacaria, RS, na safra 2005, com parcelas de 3 x 5 m, em delineamento de blocos casualizados, com quatro repetições. Foram avaliadas três cultivares, sendo aplicados dois fungicidas por cultivar: BRS Campeiro (trifenil hidróxido de estanho e azoxistrobina + ciproconazole), IPR Uirapuru (tiofanato metílico + clorotalonil e tetraconazole) e BRS Valente (trifloxystrobina + propiconazole e tebuconazole). Os critérios para a aplicação foram: calendário fixo (CF), estádio de desenvolvimento (ED) e limiar de dano econômico (LDE) de 10 % e 20 % de incidência. Semanalmente avaliou-se a incidência e a severidade, coletando-se 20 trifólios ao acaso por parcela, desde o primeiro trifólio até a maturação fisiológica. Os valores de incidência e severidade foram submetidos à área abaixo da curva de progresso de doença (AACPD). Foram colhidas manualmente todas as plantas em um metro quadrado no centro de cada parcela para o cálculo da produtividade e para a patologia dos grãos. Por último, foi realizada uma análise econômica, avaliando os custos dos seis fungicidas utilizados neste trabalho, o amassamento feito por três tipos de pulverizadores (montado, rebocado e um autopropelido modelo Uniport) e os custos fixos e variáveis do trator. Os dados da AACPD, produtividade, massa de mil grãos e patologia de sementes foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey a 5 % de probabilidade. No CF e ED foram realizadas três aplicações de fungicidas, sendo que as duas primeiras com os fungicidas direcionados à antracnose e mancha angular e a última aplicação, com os fungicidas para a ferrugem. No LDE 10 % e LDE 20 % foi realizada somente uma aplicação com os fungicidas direcionados para a ferrugem. Devido à sanidade das sementes, uso da rotação de culturas e aliada à irregularidade das precipitações pluviais, não foi detectado antracnose e mancha angular em nenhuma época de avaliação. Dentre as doenças fúngicas, detectou-se somente a ferrugem a partir da quarta avaliação. Os dados da AACPD para ferrugem demonstraram que a intensidade da doença nas três cultivares foram baixos. A cultivar IPR Uirapuru foi mais suscetível à ferrugem, enquanto a BRS Campeiro mais tolerante. Não foram detectadas diferenças significativas no rendimento de grãos e na massa de mil grãos entre os tratamentos nas três cultivares de feijão. Na patologia dos grãos, não foram detectados os fungos Colletotrichum lindemunthianum e Phaeoisariopsis griseola, indicando que a sanidade dos grãos foi um reflexo da sanidade das plantas durante período de cultivo. Na produtividade, o ganho obtido na cultivar BRS Campeiro (67 kg ou R$ 78,15), não cobre o custo de uma única entrada para a aplicação dos fungicidas testados na lavoura. Na IPR Uirapuru, com maior ganho econômico (124 kg ou R$ 144,66), o custo do controle foi coberto somente com uma aplicação e em determinados casos, a considerar o fungicida e o tipo de pulverizador. Concluiu-se que aplicações preventivas no C.F. e E.D são desnecessárias quando a fonte de inoculo primário da antracnose e mancha angular for eliminada e a intensidade da ferrugem for baixa

Submitted by Gabriela Lopes (gmachadolopesufpel@gmail.com) on 2018-07-27T18:52:58Z No. of bitstreams: 1 DISSERTAÇÃO ESTEFANIA_JULIA_DIERINGS_SOUZA_FINAL.pdf: 1787909 bytes, checksum: 80c02458a0ad77d346a0b43ad9d441e1 (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2018-07-31T18:55:04Z (GMT) No. of bitstreams: 1 DISSERTAÇÃO ESTEFANIA_JULIA_DIERINGS_SOUZA_FINAL.pdf: 1787909 bytes, checksum: 80c02458a0ad77d346a0b43ad9d441e1 (MD5) / Made available in DSpace on 2018-07-31T18:55:04Z (GMT). No. of bitstreams: 1 DISSERTAÇÃO ESTEFANIA_JULIA_DIERINGS_SOUZA_FINAL.pdf: 1787909 bytes, checksum: 80c02458a0ad77d346a0b43ad9d441e1 (MD5) Previous issue date: 2018-06-14 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / O uso de farinha de arroz é uma alternativa muito utilizada em produtos de panificação sem glúten, contudo a composição e características dos produtos depende das particularidades das farinhas utilizadas em sua elaboração. Objetivou-se com o estudo avaliar os efeitos de farinhas mistas de arroz, com diferentes teores de amilose, e feijão-caupi sobre propriedades tecnológicas, nutricionais e sensoriais de biscoitos tipo cookie. Para tanto, foram analisadas as propriedades das farinhas, como composição proximal, solubilidade e absorção de água, atividade de água, proteína solúvel e digestibilidade proteica. Os parâmetros de qualidade de biscoito tipo cookie como composição proximal, atividade de água, proteína solúvel, digestibilidade proteica, espessura, diâmetro, fator de dispersão, textura, cor, perfil de aminoácidos e análise sensorial com os testes de preferência, aceitação e intenção de compra foram avaliados. Os biscoitos tipo cookies preparados com a formulação contendo farinha de arroz de alto (BRS Catiana) e médio teor de amilose (EEA 406) apresentaram, respectivamente os menores valores de dureza enquanto que os elaborados com farinhas de arroz baixo teor de amilose (Cateto) apresentaram maior fraturabilidade. Os biscoitos tipo cookies elaborados com farinha de arroz de baixo teor de amilose (cateto) apresentaram sensorialmente maior dureza e mais escuros. Os biscoitos tipo cookies elaborados com farinha de arroz de alto teor de amilose (BRS Catiana) apresentaram coloração mais clara, menor dureza e maior preferência. Os atributos odor e sabor não apresentam diferença entre as formulações. / The use of rice flour is a widely used alternative in gluten-free baking products, however the composition and characteristics of the products depends on the particularities of the flour used in its preparation. The objective of this study was to evaluate the effects of mixed rice flour with different levels of amylose and cowpea on technological, nutritional and sensory properties of cookie. The properties of flours, such as proximal composition, solubility and water absorption, water activity, soluble protein and protein digestibility were analyzed. Cookie quality parameters such as proximal composition, water activity, soluble protein, protein digestibility, thickness, diameter, dispersion factor, texture, color, amino acid profile and sensory analysis with the tests of preference, acceptance and intention of were evaluated. Cookies prepared with the formulation containing high (BRS Catiana) and medium amylose (EEA 406) rice flour had the lowest values of hardness, respectively, while those made with low amylose (Cateto) rice flour, presented greater fracture. Cookies made with rice flour with low amylose content (Cateto) were sensorially harder and darker. Cookies made with high amylose (BRS Catiana) rice flour showed lighter color, less hardness and higher preference. The odor and taste attributes do not present differences between the formulations.

MSCAGR ( Plant Production) / Department of Plant Production / See the attached abstract below

Made available in DSpace on 2015-03-26T13:53:36Z (GMT). No. of bitstreams: 1 resumo.pdf: 1040999 bytes, checksum: d34325ed5a042eeb0a9a2ede4de1234f (MD5) Previous issue date: 2010-05-03 / Boron deficiency is common in Brazilian soils and may be a limiting factor for crop productivity, especially in dicotyledon species which are more demanding in this micronutrient. Tropical soils, generally, have the organic matter fraction as the main source of B, therefore, low organic matter content in that soils make them more prone to the occurrence of B deflciency to crops. The soil analysis for B availability is an important step to establish adequate techniques for correction of its deficiency which, along with the adequate supply of alI nutrients, is crucial for gains in crop productivity. Soil B availabilty is dependent on the reactions of B adsorption to soil which controls its concentration in the soil solution, where the amount available for plant absorption is present. These relationships are important for the correction of deficiencies and for preventing toxicities of B in the soil-crop system and the balance between the soil adsorbed B and the B in soil solution is important in order to have adequate supply to plants with preservation of the quality of the soil-plant-water system it has to be pointed out that in the natural soil pH range most of B is in form of molecular H3BO3, which has high mobility in the soil profile, being easily lixiviated, mainly in sandy soils. This work aimed to evaluate the response of common-beans (Phaseolus vulgaris L. cv. Majestoso) to addition of B in two soils with different texture, by using crescent doses of B from two sources, with and without addition of organic matter, making the relationship between the doses added to the soil and the soil B concentrations at different depths. The experiment was set as a factorial 2 x 2 x 2 x 5 [2 soils (sandy and clayey); two sources of B (boric acid and ulexite); with and without addition of organic material; and 5 doses of B (0.0, O.5, 1.5. 3.0 and 6.0 mgldm3)], in a completely randomized block design with three replications. The experimental unity was a 15 cm (diameter) by 42 cm (high) PVC tube filled with the soil sample, containg two common-bean seedlings. ln the floweríng stage, it was collected samples from the leaf tíssues for mineral analysis. After dry digestion of the vegetal samples B was determined by colorímetry with azomethine-H. Begining at the flowering stage, it was counted the number of flowers per plant. All plants cut at the physiologícal maturity stage, oven-dried at 65°C untill constant weight and the weight of the dry matter was recorded. Before plants cutting, the pods were collected and the number of pods per plant recorded. At the end of the experiment, it was collected a soil sample from each depth (2-7, 7-12, 12-22, 22-42 cm), and avaible B was determined to evaluate ist movement through the soil profile. The B concentration in Ieaves increased with the increase of the B doses applied to soil (linear and quadratic responses) independently of the soiI type, B source, or organic matter addition. These results are in accordance with the increase of B recovered from soil by the extractors HCl 50 mmol/L and hot CaCl2 5 mmol/L. The values of the linear correlation coefficients between B content in leaves and B recovered by the extractors from soil corroborate the observed toxicity symptoms in the plants at the higher B doses. In generaL the increase in the B dose added to soil, resulted in higher concentrations of available B extracted with hot CaCl2 5 mmol/L in the different depths, indicating the B mobility through the soiI profile. These values were Iower in the clayey soil due to the higher interaction between B and the surface charges of the clay fraction. / O boro se apresenta, em geral deficiente na maioria dos solos brasileiros, comprometendo o potencial produtivo das culturas em geral e das espécies dicotiledôneas em particular, as quais são mais exigentes nesse elemento. Nos solos tropicais, em geral, a matéria orgânica constitui a principal fonte de B, sendo aqueles com baixo teor de matéria orgânica, mais propensos à deficiência desse elemento. A análise da disponibilidade de B em solos é fundamental para que se busque a correção de deficiências, o que, juntamente com o aporte adequado dos outros nutrientes, possibilita ganhos de produtividade. Essa disponibilidade de B está diretamente relacionada à sua adsorção ao solo que, por sua vez, controla a concentração de B na solução do solo. Essa adsorção alterará o que está disponível, na solução do solo, para absorção pela planta, sendo importante tanto na correção de deficiências quanto na prevenção de toxidez. Esse equilíbrio entre o B adsorvido e o B em solução no solo deve ser considerado ao se buscar o suprimento do nutriente para as plantas, de modo a se garantir boa produtividade com preservação da qualidade do sistema solo-água-planta. Vale ressaltar que o B na faixa de pH natural da maioria dos solos, se encontra na sua forma indissociada, uma das razões para a grande mobilidade que este elemento apresenta ao longo do perfil dos solos, podendo sofrer lixiviaçào, em especial nos solos arenosos. Os objetivos deste trabalho foram avaliar a resposta do feijoeiro (Phaseolus vulgarís L. cv. Majestoso) à disponibilidade de B em dois solos de diferentes classes texturais, fertilizados com duas fontes de B em doses crescentes, sem e com adição de matéria orgânica, e estudar a movimentação do B no solo, relacionando as doses às concentrações de B, em diferentes profundidades. O experimento foi conduzido em esquema fatorial (2 x 2 x 2 x 5) com 2 tipos de solo (arenoso e argiloso); sem e com adição de resíduo orgânico; 2 fontes de B (ácido bórico e ulexita); e 5 doses de B (0,0, 0,5, 1,5,3,0 e 6,0 mgldm3). O delineamento foi em blocos casualizados com três repetições. A unidade experimental foi constituída de amostra de solo em tubo de PVC de 15 cm de diâmetro e 42 cm de altura, contendo duas plantas de feijão. No estádio de florescimento (R6), foi retirada uma amostra de tecido foliar para análise da composição mineral. A análise de B foi feita após digestão, por via seca, da amostra vegetal, fazendo-se a dosagem, no extrato, por colorimetria com azometina-H. A partir do florescimento foi realizada a contagem do número de flores por planta. Todas as plantas foram colhidas na maturação fisiológica dos grãos (R9) e levadas à estufa de circulação forçada de ar. Após secagem a 65 °C até atingir peso constante, foi determinado a massa de matéria seca. Antes do corte das p!antas, as vagens foram colhidas, anotando-se o número de vagens por planta. Ao fim do experimento foi coletada uma amostra de solo a cada profundidade (2-7, 7-12, 12-22, 22-42 cm), sendo determinado o teor de B disponível para avaliar a sua movimentação no solo. Houve aumento do teor de B na folha com o aumento das doses aplicadas (respostas linear e quadrática), independente da classe de solo, da fonte utilizada, e da adição ou não de resíduo orgânico. Esses resultados coincidiram com o aumento dos teores de B disponível recuperados pelos dois extratores (HCl 50 mmol/L e CaCl2 5 mmol/L fervente). A magnitude dos valores dos coeficientes de correlação linear simples entre o conteúdo de B na folha e teor de B recuperado pelos extratores corroboram com a observação dos sintomas de toxidez observados nas plantas nas maiores doses aplicadas. Em geral, com o aumento das doses de B adicionadas ao solo, houve aumento nos teores de B disponível (CaCl2 5 mmoI/L fervente) encontrados nas diferentes profundidades (anéis), o que indica mobilidade do elemento ao longo do perfil do solo. Esses teores de B foram menores para o solo argiloso devido a maior interação deste elemento com as cargas da superfície da fração argila.

A identificação de constituições genéticas de feijão (Phaseolus vulgaris L.) tolerantes à deficiência hídrica (DH) é de grande importância para os programas de melhoramento genético. O objetivo deste trabalho foi caracterizar genótipos de feijão em resposta à DH no estádio reprodutivo por meio de análises fisiológicas, agronômicas e moleculares. O experimento foi conduzido em casa de vegetação, em delineamento de blocos ao acaso com quatro repetições, utilizando 10 cultivares: ANFC 9, ANFP 110, BRS Esplendor, BRSMG Realce, IPR Siriri, IPR Tangará, IPR Tuiuiú, IPR Uirapuru, IAC Imperador e IAC Milênio em duas condições de irrigação: plantas irrigadas durante todo o ciclo, e plantas submetidas a supressão de irrigação no estádio reprodutivo (R7), até o solo atingir 16% da capacidade de campo, quando a irrigação foi reestabelecida. Nos últimos quatro dias de estresse foram analisadas as trocas gasosas. No último dia de estresse foi analisado percentual de estômatos fechados na face abaxial das folhas durante o dia, e coletadas plantas para as seguintes análises: massa fresca e massa seca das folhas, hastes e legumes, e conteúdo de prolina em folhas e raízes. Na maturidade fisiológica foi realizada a colheita e avaliados os componentes do rendimento e estimada a produtividade de grãos. Adicionalmente, visando identificar polimorfismo sequencias gênicas a promotoras relacionadas com a tolerância à DH, foram testados sete pares de primers no grupo de genótipos estudado. O índice de suscetibilidade à seca (ISS) variou de 0,65 a 1,10 no grupo de genótipos, sendo os menores valores observados para IAC Imperador (0,65) e BRS Esplendor (0,87) indicando a capacidade destes dois genótipos em manter a produtividade de grãos na condição de estresse. Todos os genótipos avaliados apresentaram redução nos componentes do rendimento quando estressados pela DH. IAC Imperador (43,4%) e BRS Esplendor (60,6%) apresentaram as menores reduções na produtividade e mantiveram cerca de 50% dos estômatos fechados em todos os horários analisados no último dia de DH. IAC Imperador apresentou maior eficiência no uso da água e taxa de assimilação de CO2 na DH. IPR Tuiuiú, IPR Tangará e IAC Imperador apresentaram as maiores concentrações de prolina nas raízes. Na condição de DH, houve forte correlação positiva significativa entre percentual de estômatos fechados com número de grãos por legume (0,696) e massa fresca da folha (0,731), sendo o percentual máximo de estômatos fechados 73,71% em DH. O caractere acúmulo de prolina na raiz foi o que mais contribuiu para a divergência entre os genótipos na DH, porém, nem sempre os genótipos que mais acumularam prolina foram os mais tolerantes. Os polimorfismos no DNA de sequências codificadoras e promotoras dos fatores de transcrição estudados neste experimento não discriminaram os genótipos tolerantes dos sensíveis à DH. / The identification of genotypes for drought tolerance has a great importance in breeding programs. The aim of this study was to characterize genotypes of beans in response to drought tolerance in different reproductive stages through physiologic, agronomic and molecular analysis. The experiment was conducted in greenhouse, using a randomized block design with four replicates; 10 cultivars: ANFC 9, ANFP 110, BRS Esplendor, BRSMG Realce, IPR Siriri, IPR Tangará, IPR Tuiuiu, IPR Uirapuru, IAC Imperador and IAC Milênio under two conditions of irrigation: plants irrigated during their entire life cycle, and plants under irrigation suppression in the reproductive stage (R7) until 16% of field capacity, when the irrigation was restored. In the last four days of stress, the gas exchanges were analyzed, and in the last day of stress was analyzed the percentage of closed stomata in the abaxial surface of the leaves, collected in different times of the day (9h, 12h, 15h and 18h). Additionally, plant samples were collected for the following analysis: fresh and dry mass of leaves, stems and legumes, and proline content in leaves and roots. The plants were harvested at the physiological maturity and the yield components and grain yield were determined. In addition, in order to identify polymorphisms in the sequences of promoters and genes related to drought, seven pairs of primers were tested on the group of genotypes. The drought susceptibility indexes (ISS) ranged from 0.65 to 1.10 in the group of genotypes, which the lowest values observed were for IAC Imperador (0.65) and BRS Esplendor (0.87), indicating the ability of these two genotypes to maintain grain yield under water stress condition. All genotypes showed reduction in yield components under water stress. IAC Imperador (43.4%) and BRS Esplendor (60.6%) had the lowest reductions in productivity and kept about 50% of the stomata closed during all the different times evaluated at last day of irrigation suppression. IAC Imperador showed greater water use efficiency and CO2 assimilation rate under drought stress. IPR Tuiuiú, IPR Tangará and IAC Imperador had the highest proline concentrations in the roots. Under water stress condition, there was a strong positive correlation (0.696) between the percentage of stomata closed with the number of grains per plant (0.696) and the fresh mass of leaves (0.731), the maximum percentage of stomata closed 73.71% in water stress. The accumulation of proline in the root was the character that most contributed to the divergence between the genotypes under water deficit, but not always the genotypes that have accumulated more proline were the most tolerant. The polymorphisms in DNA of coding and promoting sequences of transcription factors studied in this experiment did not discriminate tolerant genotypes from the sensitive ones to water stress.