Wong, Sze Ki. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 87-106). / Abstracts in English and Chinese. / Acknowledgements --- p.i / Abstract --- p.ii / Abstract in Chinese --- p.iv / Table of contents --- p.V / List of figures --- p.ix / List of abbreviations --- p.X / Chapter Chapter 1. --- General introduction --- p.1 / Chapter Chapter 2. --- Literature review --- p.6 / Chapter 2.1. --- Pathogenic mechanisms of Vibrio species in fish --- p.7 / Chapter 2.1.1. --- Introduction --- p.7 / Chapter 2.1.2. --- Adhesion --- p.7 / Chapter 2.1.3. --- Invasion --- p.8 / Chapter 2.1.4. --- Proliferation --- p.9 / Chapter 2.2. --- Vibrio virulence factors --- p.12 / Chapter 2.2.1. --- Introduction --- p.12 / Chapter 2.2.2. --- Hemolysin --- p.12 / Chapter 2.2.3. --- Protease --- p.14 / Chapter 2.2.4. --- Siderophore --- p.15 / Chapter 2.2.5. --- Lipopolysaccharide --- p.15 / Chapter 2.3. --- General apoptotic pathways --- p.17 / Chapter 2.3.1. --- Introduction --- p.17 / Chapter 2.3.2. --- Extrinsic apoptotic pathway --- p.17 / Chapter 2.3.2.1. --- Death receptor signaling apoptosis --- p.17 / Chapter 2.3.2.1.1. --- Fas signaling pathway --- p.18 / Chapter 2.3.2.1.2. --- TNF-R1 signaling pathway --- p.19 / Chapter 2.3.2.1.3. --- TRAIL receptors signaling pathway --- p.20 / Chapter 2.3.2.2. --- Growth factor receptor signaling apoptosis --- p.21 / Chapter 2.3.3. --- Intrinsic apoptotic pathway --- p.21 / Chapter 2.3.3.1. --- Mitochondrial apoptotic pathway --- p.21 / Chapter 2.3.3.1.1. --- Cyto c --- p.22 / Chapter 2.3.3.1.2. --- Smac/DIABLO --- p.22 / Chapter 2.3.3.1.3. --- Omi/HtrA2 --- p.22 / Chapter 2.3.3.1.4. --- AIF and endo G --- p.23 / Chapter 2.3.3.1.5. --- Bcl-2 family --- p.23 / Chapter 2.3.3.1.6. --- Mitochondrial membrane permeabilization (MMP) --- p.23 / Chapter 2.3.3.2. --- p53-regulated apoptotic pathway --- p.24 / Chapter 2.3.3.3. --- Endoplasmic reticulum (ER) stress-induced apoptotic pathway --- p.25 / Chapter 2.4. --- Membrane vesiculation in erythrocytes --- p.26 / Chapter 2.4.1. --- Introduction --- p.26 / Chapter 2.4.2. --- Induction of vesiculation --- p.26 / Chapter 2.4.3. --- Contents of vesicles --- p.28 / Chapter 2.4.4. --- Mechanisms involved during vesiculation --- p.29 / Chapter 2.4.5. --- Correlation between apoptosis and membrane vesiculation in erythrocytes --- p.31 / Chapter 2.4.6. --- Reasons for vesiculation --- p.31 / Chapter Chapter 3. --- "Induction of apoptosis by Vibrio alginolyticus thermolabile hemolysin (TLH) in blood cells of silver sea bream, Sparus sarba" --- p.33 / Chapter 3.1. --- Abstract --- p.34 / Chapter 3.2. --- Introduction --- p.34 / Chapter 3.3. --- Materials and methods --- p.36 / Chapter 3.3.1. --- Experimental fish --- p.36 / Chapter 3.3.2. --- Whole blood preparation --- p.37 / Chapter 3.3.3. --- Preparation of V. alginolyticus TLH --- p.37 / Chapter 3.3.4. --- "Caspase-3, -8, -9/6 fluorescent assay" --- p.38 / Chapter 3.3.5. --- TUNEL assay --- p.39 / Chapter 3.3.6. --- Apoptotic DNA ladder assay --- p.40 / Chapter 3.3.7. --- Statistical analysis --- p.41 / Chapter 3.4. --- Results --- p.42 / Chapter 3.4.1. --- "Increase of caspase-3, -8, -9/6 activities" --- p.42 / Chapter 3.4.2. --- Detection of DNA fragmentation by TUNEL assay --- p.44 / Chapter 3.4.3. --- Detection of DNA fragmentation by apoptotic DNA ladder assay --- p.44 / Chapter 3.5. --- Discussion --- p.46 / Chapter Chapter 4. --- "Occurrence of membrane vesiculation, apoptosis and post-apoptotic necrosis after exposure to Vibrio alginolyticus thermolabile hemolysin (TLH) in erythrocytes of silver sea bream, Sparus sarba" --- p.51 / Chapter 4.1. --- Abstract --- p.52 / Chapter 4.2. --- Introduction --- p.52 / Chapter 4.3. --- Materials and methods --- p.54 / Chapter 4.3.1. --- Experimental fish --- p.54 / Chapter 4.3.2. --- Whole blood preparation --- p.54 / Chapter 4.3.3. --- Preparation of V. alginolyticus TLH --- p.55 / Chapter 4.3.4. --- Light microscopy --- p.55 / Chapter 4.3.5. --- Transmission electron microscopy (TEM) --- p.56 / Chapter 4.3.6. --- Measurement of membrane vesiculation - acetylcholinesterase (AChE) assay --- p.56 / Chapter 4.3.7. --- Measurement of necrosis - hemoglobin colorimetric assay --- p.57 / Chapter 4.3.8. --- Apoptotic DNA ladder assay --- p.58 / Chapter 4.3.9. --- Flow cytometry --- p.59 / Chapter 4.3.10. --- Statistical analysis --- p.59 / Chapter 4.4. --- Results --- p.60 / Chapter 4.4.1. --- Ultrastructural changes in red blood cells after exposure to TLH --- p.60 / Chapter 4.4.2. --- Changes of cell population in size and granularity after exposure of TLH --- p.67 / Chapter 4.4.3. --- Effect of TLH dosage on necrosis and DNA fragmentation --- p.72 / Chapter 4.4.4. --- "Occurrence of membrane vesiculation, necrosis and DNA fragmentation in cells exposed to TLH" --- p.72 / Chapter 4.5. --- Discussion --- p.76 / Chapter Chapter 5. --- General conclusions --- p.82 / References --- p.87

The main objective of this thesis was to assess food intake by bream, depending on the onset and end of spawning period. To meet the objectives of a practical experiment was carried out. This experiment was run on the dams Hamry and Brno in the years 2008 and 2009. Subsequently it was determined by standard methods. These methods (indirect, food frequency, filling index, index of superiority and gonadosomatic index. Subsequently, an analysis of stomach.

Climate changes alter species composition in both aquatic and terrestrial ecosystems. For establishment of species in a fish community, dispersal barriers, habitat structure and interactions between species act as a filter. The aim of this study was to examine changes in the fish community in Lake Tåkern, Sweden, by repeating a test-fishing in a lake 40 years after a previous test-fishing. Special interest was directed towards the possible establishment of bream (Abramis brama) and (Cyprinus carpio) which recently had been found in the lake. In addition, a decline of the low oxygen tolerant species crucian carp (Carassius carassius) and tench (Tinca tinca) was expected. Test-fishing was performed in June and August 2019 and combined with analyses of Environmental-DNA from samples in September 2019. A comparison with the average catch of 1978-1979 together showed that all cyprinid species had decreased in 2019. The hypoxic tolerant crucian carp decreased 16-fold in numbers, which was expected due to fewer events of cold winters and hypoxia. One bream was caught from the test-fishing and was also detected through eDNA-analysis. The ecological status of the fish community was classified as moderate but showed a high proportion of piscivore percids contra cyprinids, compared to six other shallow lakes in Sweden with similar characteristics. The high proportion of piscivore percids is probably contributing to the stability of the present clear-water state of the lake.

The sexual segregation, defined as different use of space by sexes is widespread phenomenon in the animal tree of life. As a result of segregation there is a different use of resources such as habitat, prey items or different threat by predation. Nevertheless it is not well studied in fish taxa except in marine environment. The common and widespread freshwater species such as a bream (Abramis brama), a bleak (Alburnus alburnus) and a roach (Rutilus rutilus) so far have not never been studied from the perspective of sexual segregation too. This thesis aims to examine the sexual segregation of these species and to test premises of sexual segregation such as sexual size dimorphism, age composition and differences in food. Afterwards the different space use of sexes in the Římov reservoir was tested. The gillnet sampling was used to collect data. Gillnets were installed to four localities in longitudinal gradient of reservoir in epilimnion where is the highest abundance of fish. Sampling was performed in August from 2009 to 2016. Results of this thesis show that females and males have different life histories and that bleak and roach are significantly bigger and older than males. The size dimorphism in bream was confirmed but not age difference between sexes. Sexes of bream had different diet. Males...

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第21827号 / 農博第2340号 / 新制||農||1067(附属図書館) / 学位論文||H31||N5199(農学部図書室) / 京都大学大学院農学研究科応用生物科学専攻 / (主査)教授 佐藤 健司, 准教授 豊原 治彦, 准教授 田川 正朋 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

During the last two hundred years many wetlands and streams in Sweden have been lowered to provide land for agriculture and forestry. Some of the coastal wetlands and streams probably served as spawning sites for certain species of fish in the Baltic Sea, but the present situation is not well known. As standardized methods to monitor fish in running waters are carried out during late summer or autumn, there is a need for methods to sample spring-spawning fish in these streams. The aim of this study was to survey the lower kilometer stretch of Hammerstaån in Stockholm, to investigate the extent to which the stream is used by spring-spawning fish. Furthermore, the study aims to evaluate a new type of survey fishing gill net called strömöversiktsnät (SÖN). This was done by comparing the catch of fish by SÖN with that of fyke nets. The comparison of the types of gear included number of fish caught, on a catch per unit effort (CPUE) basis, number of species and the size distributions. The study included 14 days with one gill net and one fyke net at two sites, in total 28 net-days. The inventory yielded in total seventeen species among which only trout (Salmo trutta), perch (Perca fluvitalis), roach (Rutilus rutilus), burbot (Lota lota), eel (Anguilla anguilla) and pike (Esox lucius) were previously known to occur. Six spawning species, smelt (Osmerus eperlanus), vimba bream (Vimba vimba), perch (Perca fluvitalis), roach (Rutilus rutilus), bream (Abramis brama) and white bream (Blicka bjoerkna) were caught in more than 60 individuals, which was set as a limit for comparisons of size distributions. There was no significant difference in CPUE between SÖN and fyke nets. Also length distribution did not differ between SÖN and fyke nets except for the species perch and white bream were the nets caught significant smaller perch and bigger white bream than the fyke nets. The results show that investigations with SÖN or fyke nets during springtime can be of great importance to increase our knowledge about spring-spawning fish in Swedish streams and the coastal areas of the Baltic Sea.

During the last two hundred years many wetlands and streams in Sweden have been lowered to provide land for agriculture and forestry. Some of the coastal wetlands and streams probably served as spawning sites for certain species of fish in the Baltic Sea, but the present situation is not well known. As standardized methods to monitor fish in running waters are carried out during late summer or autumn, there is a need for methods to sample spring-spawning fish in these streams. The aim of this study was to survey the lower kilometer stretch of Hammerstaån in Stockholm, to investigate the extent to which the stream is used by spring-spawning fish. Furthermore, the study aims to evaluate a new type of survey fishing gill net called strömöversiktsnät (SÖN). This was done by comparing the catch of fish by SÖN with that of fyke nets. The comparison of the types of gear included number of fish caught, on a catch per unit effort (CPUE) basis, number of species and the size distributions. The study included 14 days with one gill net and one fyke net at two sites, in total 28 net-days. The inventory yielded in total seventeen species among which only trout (Salmo trutta), perch (Perca fluvitalis), roach (Rutilus rutilus), burbot (Lota lota), eel (Anguilla anguilla) and pike (Esox lucius) were previously known to occur. Six spawning species, smelt (Osmerus eperlanus), vimba bream (Vimba vimba), perch (Perca fluvitalis), roach (Rutilus rutilus), bream (Abramis brama) and white bream (Blicka bjoerkna) were caught in more than 60 individuals, which was set as a limit for comparisons of size distributions. There was no significant difference in CPUE between SÖN and fyke nets. Also length distribution did not differ between SÖN and fyke nets except for the species perch and white bream were the nets caught significant smaller perch and bigger white bream than the fyke nets. The results show that investigations with SÖN or fyke nets during springtime can be of great importance to increase our knowledge about spring-spawning fish in Swedish streams and the coastal areas of the Baltic Sea.

The feeding experiment was performed with fry of vimba bream (Vimba vimba) during the first 24 days from the beginning of exogenous nutrition. Six diets were used: algae (Chlorella vulgaris), starter fodder BioOptimal 0,8 mm by Biomar company ? for cyprinids, live nauplii of Artemia salina, combination of alga and starter fodder BioOptimal, combination of alga and nauplii of Artemia salina, combination of starter fodder BioOptimal and nauplii of Artemia salina. Individual weight, total lenght, survival rate of larvae, specific growth rate (SGR) and conditions of the fry (condition coefficient according to Fulton ? FWC) were observed during the experiment. The results were compared statistically using the method of one ? way analysis of variance (ANOVA ? = 0,05; Microsoft Excel).

Conselho Nacional de Desenvolvimento Científico e Tecnológico / The essential oil (EOs) extracted from plants Hesperozygis ringens and Lippia alba possess anesthetic and sedative properties and is an alternative to traditional anesthetics used in aquaculture for ease of handling and/or reduce stress. In this sense, the study aimed to investigate the effects of these EOs on the physiology of fish, through physiological, biochemical and endocrine indicators. In the article 1 was determined (a) the anesthetic activity of the EOs of H. ringens (EOHR) and L. alba (EOLA) and (b) its effects on silver catfish (Rhamdia quelen) after induction and recovery from anesthesia. Fish were subjected to one of the following treatments for each EO: basal group, control or anesthetized (150, 300 or 450 uL L-1 EO), evaluating the ventilatory rate (VR) during the induction period and thereafter transferred to anesthetics-free tanks for recovery from anesthesia. At 0, 15, 30, 60 and 240 min of recovery, samples of plasma and gills were collected to measure metabolic indicators and ionregulatory enzymes, respectively. In the article 2, the effects of prolonged exposure to low EOHR concentrations were studied on silver catfish. After 6 h of exposure to 0 (control), 30 or 50 uL L-1 EOHR added to water, it was analyzed: VR, metabolic indicators of stress in plasma, enzyme activity in liver, and expression of pituitary hormones (growth hormone - GH, prolactin - PRL and somatolactina - SL). In the manuscript, (a) evaluated the effectiveness of anesthesia EOLA on gilthead sea bream (Sparus aurata) and (b) we investigated the effects of 35 uL L-1 EOLA and 2-phenoxyethanol (2-PHE) on the stress response in gilthead sea bream undergoing persecution. After 4 h of exposure, the plasma was sampled (for the determination of cortisol, metabolites and osmolality), brain and pituitary (to evaluate the expression of endocrine indicators). In the article 1, anesthesia with EOs caused changes in some parameters measured in silver catfish, but did not prevent the restoration of most of the indicators assessed after 240 min of recovery. In the article 2, 50 uL L-1 EOHR led to an increase of glucose, lactate, protein and osmolality, as well as an increase in metabolic enzyme activity and reduced expression of GH and SL. In the manuscript, gilthead sea bream exposed to EOLA, stressed or not, exhibited higher levels of cortisol, glucose, lactate and osmolality. EOLA exposure added to the stress reduced the expression levels of CRH-BP (corticotropin releasing hormone bound to protein). PRL expression was reduced in the stressed control group and after exposure to EOLA and 2-PHE in fish not stressed. Higher expression of pro-opiomelanocortin (POMC) "a" and "b" were observed in fish stressed and exposed to EOLA and 2-PHE, respectively. We conclude that: (1) the EOLA is more efficient for silver catfish that EOHR in anesthesia concentrations; (2) for sedating the fish, it is recommended 30 uL EOHR L-1 (or less); (3) the EOLA was effective as an anesthetic for gilthead sea bream at 100-300 uL L-1, but for 4 h exposure, the 2-PHE was more effective in preventing the stress response. / Os óleos essenciais (OEs) extraídos das plantas Hesperozygis ringens e Lippia alba possuem propriedades anestésica e sedativa, constituindo uma alternativa aos anestésicos tradicionalmente usados em aquicultura para facilitar o manejo e/ou reduzir o estresse. Neste sentido, o estudo teve por objetivo investigar os efeitos desses OEs sobre a fisiologia de peixes, através de indicadores fisiológicos, bioquímicos e endócrinos. No artigo 1 determinou-se (a) a atividade anestésica dos OEs de H. ringens (OEHR) e L. alba (OELA) e (b) seus efeitos em jundiá (Rhamdia quelen) depois da indução e recuperação da anestesia. Os peixes foram submetidos a um dos seguintes tratamentos para cada OE: grupo basal, controle ou anestesiado (150, 300 ou 450 μL L-1 OE), avaliando-se a taxa ventilatória (TV) durante o período de indução e, posteriormente, transferidos para aquários sem anestésicos para recuperação da anestesia. Nos tempos 0, 15, 30, 60 e 240 min de recuperação foram realizadas amostragens de plasma e brânquias para medir indicadores metabólicos e enzimas ionorregulatórias, respectivamente. No artigo 2, os efeitos da exposição prolongada de jundiás a baixas concentrações do OEHR foram estudados. Após 6 h de exposição a 0 (controle), 30 ou 50 μL L-1 OEHR adicionado à água, analisou-se: TV, indicadores metabólicos e de estresse em plasma, atividade enzimática em fígado e expressão de hormônios hipofisários (hormônio do crescimento - GH, prolactina - PRL e somatolactina - SL). No manuscrito (a) avaliou-se a eficácia anestésica do OELA em dourada (Sparus aurata) e (b) investigaram-se os efeitos de 35 μL L-1 de OELA e 2-fenoxietanol (2-PHE) sobre a resposta ao estresse em douradas submetidos à perseguição. Após 4 h de exposição, foram amostrados plasma (para determinação dos níveis de cortisol, metabólitos e osmolalidade), cérebro e hipófise (para avaliar a expressão de indicadores endócrinos). No artigo 1, a anestesia com os OEs provocou alterações em alguns parâmetros medidos em jundiás, mas não impediu a restauração da maioria dos indicadores avaliados após 240 min de recuperação. No artigo 2, 50 μL L-1 do OEHR provocou a elevação dos níveis de glicose, lactato, proteína e osmolalidade, bem como aumento na atividade de enzimas metabólicas e redução na expressão do GH e SL. No manuscrito, douradas expostos ao OELA, estressados ou não, exibiram maiores níveis de cortisol, glicose, lactato e osmolalidade. A exposição ao OELA somado ao estresse reduziu os níveis de expressão de CRH-BP (hormônio liberador de corticotrofina ligado à proteína). A expressão de PRL foi reduzida no grupo controle estressado e após a exposição ao OELA e 2-PHE em peixes não estressados. Maiores expressões de pro-opiomelanocortina (POMC) a e b foram observadas em peixes estressados e expostos ao OELA e 2-PHE, respectivamente. Conclui-se que: (1) o OELA é mais eficiente para jundiás que o OEHR em concentrações para anestesia; (2) para sedar os peixes, recomenda-se 30 μL L-1 do OEHR (ou menos); (3) o OELA foi eficaz como anestésico para dourada entre 100-300 μL L-1, mas para 4 h de exposição o 2-PHE foi mais eficiente em prevenir a resposta ao estresse.

[EN] The state of the Spanish aquaculture Sea bream, Sea bass and Meagre produced in fish farms in the Spanish Mediterranean coast is analyzed setting as objective the analysis of the economical and financial situation of companies in the sector to characterize the successful companies. For this project a study was made during the period from 2002 to 2011, on the period before the economical crisis (2002-2007) and also during the crisis (2007-2011), to interpret the evolution of the companies from the financial and economical information extracted from their financial statements filed with the Companies Registry and are supplied by the SABI database. Economic and financial ratios of the sample aquaculture companies located in the Mediterranean coast examines representing 63% (2007), production of Sea bream, Sea bass and Meagre in Spain using statistical correlation analysis, principal components and cluster group that have allowed companies based on their financial situation. The combination of the economic and financial ratios to define a "Success Rate" of companies, it helps to know the status of each within the sector in relation to other companies, from assessing economical and financial variables if the steps taken leading to success or failure and thus becoming a key tool in making decisions at the enterprise level. The results indicate that only companies with high volume production or small well-managed companies have been able to withstand periods of economical crisis. Likewise, annual ratios of companies with best results in each period are analyzed to define the business model of success that is configured as necessary reference in the process of management in the aquaculture sector. During this time the evolution of the sales prices of the different aquatic species in Spain is also studied, from data the wholesale markets, the price observatory of the Ministerio de Agricultura, Alimentación y Medio Ambiente, and Mis Peces portal, as the price is vital for good economical performance, along with a sound technical and economical-financial management. The concept of "success rate" is new in this sector, it has developed a valid tool for studying the situation of companies within the aquaculture sector from the financial economic variables, easily accessible to the Financial Statements proceed with a reasonable degree of reliability compared to other more subjective variables. / [ES] Se analiza el estado de la acuicultura española de la dorada, lubina y corvina producidas en granjas marinas en el litoral del mar Mediterráneo español, fijándose como objetivo el análisis de la situación económico-financiera de las empresas del sector para caracterizar a las empresas de éxito. Para ello se realiza un estudio durante el periodo desde el año 2002 hasta el 2011, espacio de tiempo previo a la crisis económica (2002-2007) y durante la crisis (2007-2011), para analizar la evolución de las empresas a partir de la información económica financiera extraída de sus estados contables depositados en el Registro Mercantil y que son suministrados por la Base de Datos SABI. Se examinan los ratios económico-financieros de la muestra tomada de empresas de acuicultura ubicadas en el litoral Mediterráneo que representan el 63 % (2007), de la producción de dorada, lubina y corvina en España mediante análisis estadísticos de correlación, componentes principales y clúster, que han permitido agrupar a las empresas en función de su situación económico-financiera. La combinación de los ratios económico-financieros permite definir un "Índice de Éxito" de las empresas, que ayuda a conocer la situación de cada una de ellas dentro del sector en relación al resto de empresas, evaluando a partir de variables económico financieras si la gestión realizada lleva al éxito o al fracaso y convirtiéndose así en una herramienta clave en la toma de decisiones a nivel empresarial. Los resultados indican que solo empresas con gran volumen de producción o pequeñas empresas bien gestionadas, han sido capaces de soportar el periodo de crisis económica. Asimismo, se analizan los ratios anuales de las empresas con mejores resultados en cada periodo para definir el modelo de empresa de éxito que se configura como referencia necesaria en el proceso de gestión en el sector de la acuicultura. Durante este tiempo también se estudia la evolución de los precios de venta de las diferentes especies acuícolas en España, a partir de los datos de la Red de Mercas, el Observatorio de Precios del Ministerio de Agricultura, Alimentación y Medio Ambiente, y el portal Mis Peces, pues el precio de venta es vital para obtener buenos resultados económicos, además de una buena gestión técnica y económico-financiera. El concepto del "Índice de Éxito" resulta novedoso en este sector, pues se ha desarrollado una herramienta válida para estudiar la situación de las empresas dentro del sector acuícola a partir de las variables económicas financieras, fácilmente accesibles al proceder de los Estados Contables y con un razonable grado de fiabilidad frente a otras variables más subjetivas. / [CAT] S'analitza l'estat de l'aqüicultura espanyola de l'orada, llobarro i corbina produïdes en granges marines al litoral del mar Mediterrani espanyol, establint com a objectiu l'anàlisi de la situació económica i financera de les empreses del sector per tal de caracteritzar les empreses d'èxit. Per a això, es realitza un estudi durant el període des de l'any 2002 fins al 2011, que inclou un espai de temps previ a la crisi econòmica (2002-2007) i altre durant la crisi (2007-2011), per analitzar l'evolució de les empreses a partir de la informació econòmica-financera extreta dels seus estats comptables dipositats en el Registre Mercantil, i que són subministrats per la base de dades SABI. S'examinen les ràtios econòmicofinanceres d'un conjunt d'empreses d'aqüicultura ubicades al litoral Mediterrani, que representen el 63% (2007), de la producció d'orada, llobarro i corbina a Espanya, mitjançant diferents anàlisis estadístics, com l'anàlisi de correlació, components principals i clúster, que han permès agrupar les empreses en funció de la seva situació economicofinancera. La combinació de les ràtios economicofinanceres permet definir un "Índex d'Èxit" de les empreses, que ajuda a conèixer la situació de cadascuna d'elles dins del sector en relació a la resta d'empreses, avaluant a partir de variables econòmico-financeres si la gestió realitzada duu a l'èxit o al fracàs, convertint-se així en una eina clau en la presa de decisions a nivell empresarial. Els resultats indiquen que només empreses amb gran volum de producción, o xicotetes empreses ben gestionades, han estat capaços de suportar el període de crisi econòmica. Així mateix, s'analitzen les ràtios anuals de les empreses amb millors resultats en cada període per definir el model d'empresa d'èxit, esdevenenint aquest una referència necessària en el procés de gestió en el sector de l'aqüicultura. També s'estudia l'evolució dels preus de venda de les diferents espècies aqüícoles a Espanya durant el període entre 2002 i 2011, a partir de les dades de la Red de Mercas, l'Observatori de Preus del Ministeri d'Agricultura, Alimentació i Medi Ambient i el portal Mis Peces, ja que el preu de venda és vital per obtenir bons resultats econòmics, a més de la realització d'una bona gestió tècnica i econòmicofinancera. El concepte d'"Índex d'Èxit" és nou en aquest sector, i representa una eina vàlida per estudiar la situació de les empreses dins del sector aqüícola a partir de les variables econòmico-financeres, fàcilment accessibles ja que procedeixen dels estats comptables, i amb un raonable grau de fiabilitat en comparació amb altres variables més subjectives. / Benito Alas, F. (2016). ANÁLISIS DE LA SITUACIÓN ECONÓMICA-FINANCIERA DEL SECTOR PRODUCTOR DE LA DORADA (Sparus aurata L.), LUBINA (Dicentrarchus labrax L.) Y CORVINA (Argyrosomus regius A.), EN EL LITORAL MEDITERRANEO ESPAÑOL [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/62187 / TESIS