Soil salinity is one of the major abiotic stresses that decreases agricultural crop production through imposition of both ionic and osmotic stresses. The accumulation of Na⁺ and Cl⁻ in the cytosol to toxic levels inhibits metabolism. Unlike Na⁺, less is known about Cl⁻ uptake and transport in plants. Grapevine is moderately sensitive to salinity and accumulation of toxic levels of Cl⁻ in leaves is the major reason for salt-induced symptoms. In this study Cl⁻ uptake and transport mechanism(s) were investigated in two grapevine (Vitis sp.) rootstock hybrids differing in salt tolerance: 1103 Paulsen (salt tolerant) and K 51-40 (salt sensitive). Increased external salinity caused high Cl⁻ accumulation in shoots of the salt sensitive K 51-40 in comparison to Paulsen. Measurement of ¹ ⁵ NO₃⁻ net fluxes under high salinity showed that by increasing external Cl⁻ concentrations K 51-40 roots showed reduced NO₃⁻ accumulation. This was associated with increased accumulation of Cl⁻. In comparison to Paulsen, K 51-40 showed reduced NO₃⁻ / Cl⁻ root selectivity with increased salinity, but Paulsen had lower selectivity over the whole salinity range (0-45 mM). In order to examine if root hydraulic and permeability characterisations accounted for differences between varieties, the root pressure probe was used on excised roots. This showed that the osmotic Lpr was significantly smaller than hydrostatic Lpr, but no obvious difference was observed between the rootstocks. The reflection coefficient (σ) values (0.48-0.59) were the same for both rootstocks, and root anatomical studies showed no obvious difference in apoplastic barriers of the main and lateral roots. Comparing the uptake of Cl⁻ with an apoplastic tracer, PTS (3-hydroxy-5, 8, 10-pyrentrisulphonic acid), showed that there was no correlation between Cl⁻ and PTS transport. These results indicated that by-pass flow of salts to the xylem is the same for both rootstocks (10.01±3.03 % and 12.1±1.21 %) and hence pointed to differences in membrane transport to explain difference in Cl⁻ transport to the shoot. ³ ⁶Cl⁻ fluxes across plasma membrane and tonoplast of K 51-40 and Paulsen roots showed that ³ ⁶Cl⁻ influx in root segments of Paulsen was greater than K 51-40 over the first 10 minutes. Unidirectional influx within 10 min loading time showed increases with increases in the external concentrations in both rootstocks but Paulsen had higher influx rate when compared to K 51-40. This appeared to be due to a greater Vmax. There was no significant difference in Km. It was shown that ³ ⁶Cl⁻ accumulation and transport rate to the shoot of K 51-40 was higher than that of Paulsen. Compartmental analysis of ³ ⁶Cl⁻ efflux from intact roots confirmed that the difference in influx observed between the rootstocks was consistent with the results obtained for excised roots, although the values were not exactly the same. It was also shown that the main root of Paulsen had greater contribution to ³ ⁶Cl⁻ uptake than lateral roots. ³ ⁶Cl⁻ fluxes by lateral roots were not significantly different between the rootstocks. Cl⁻ and Na⁺ distribution patterns in different root cell types were determined using the X-ray microanalysis technique. It was shown that Cl⁻ content in the hypodermis and cortical cells was higher than the other cell types in both rootstocks, but overall Cl⁻ content in the root of Paulsen was higher than K 51-40. The pericycle of the main root of Paulsen accumulated more Cl⁻ than K 51-40. It was concluded that Cl⁻ loading to the xylem was different in the rootstocks and Paulsen tended to prevent the xylem Cl⁻ loading process. Lateral roots also displayed opposite behaviour consistent with flux analysis. Membrane potential difference (PD) of the cortical cells showed a rapid and transient depolarization by adding 30 mM NaCl in both rootstocks that was followed by a gradual hyperpolarization. Depolarizations caused by 30 mM Choline-Cl, Na-MES and NaCl measured by the root surface potential method showed that Choline-Cl in K 51-40 and Na-MES in Paulsen caused greater depolarization than that of Na-MES in K 51-40 and Choline-Cl in Paulsen respectively. Assuming that PD measured in this method was the trans-root potential (TRP), it was concluded that the higher depolarization by Choline-Cl in K 51-40 can be due to higher Cl⁻ efflux rate to the xylem. Two different mechanisms were also detected for Cl⁻ transport: HATS which was observed in the range of 0.5-5 mM and a LATS in the range of 10-30 mM of the external NaCl concentration. This was consistent with the concentration dependence of Cl⁻ influx. In conclusion, evidence obtained from different experiments of this study indicated that in the grapevine rootstocks (Paulsen and K 51-40) Cl⁻ was mostly transported through the symplastic pathway. From ECl values determined for the rootstocks by the Nernst equation, a proton-driven transport system was responsible for Cl⁻ transport in both the HATS and LATS range of external NaCl concentrations. The rate of Cl⁻ transport from the root to shoot (xylem loading) was the major difference in Cl⁻ transport between the rootstocks in terms of salinity tolerance. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1339051 / Thesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food and Wine, 2008

Soil salinity is one of the major abiotic stresses that decreases agricultural crop production through imposition of both ionic and osmotic stresses. The accumulation of Na⁺ and Cl⁻ in the cytosol to toxic levels inhibits metabolism. Unlike Na⁺, less is known about Cl⁻ uptake and transport in plants. Grapevine is moderately sensitive to salinity and accumulation of toxic levels of Cl⁻ in leaves is the major reason for salt-induced symptoms. In this study Cl⁻ uptake and transport mechanism(s) were investigated in two grapevine (Vitis sp.) rootstock hybrids differing in salt tolerance: 1103 Paulsen (salt tolerant) and K 51-40 (salt sensitive). Increased external salinity caused high Cl⁻ accumulation in shoots of the salt sensitive K 51-40 in comparison to Paulsen. Measurement of ¹ ⁵ NO₃⁻ net fluxes under high salinity showed that by increasing external Cl⁻ concentrations K 51-40 roots showed reduced NO₃⁻ accumulation. This was associated with increased accumulation of Cl⁻. In comparison to Paulsen, K 51-40 showed reduced NO₃⁻ / Cl⁻ root selectivity with increased salinity, but Paulsen had lower selectivity over the whole salinity range (0-45 mM). In order to examine if root hydraulic and permeability characterisations accounted for differences between varieties, the root pressure probe was used on excised roots. This showed that the osmotic Lpr was significantly smaller than hydrostatic Lpr, but no obvious difference was observed between the rootstocks. The reflection coefficient (σ) values (0.48-0.59) were the same for both rootstocks, and root anatomical studies showed no obvious difference in apoplastic barriers of the main and lateral roots. Comparing the uptake of Cl⁻ with an apoplastic tracer, PTS (3-hydroxy-5, 8, 10-pyrentrisulphonic acid), showed that there was no correlation between Cl⁻ and PTS transport. These results indicated that by-pass flow of salts to the xylem is the same for both rootstocks (10.01±3.03 % and 12.1±1.21 %) and hence pointed to differences in membrane transport to explain difference in Cl⁻ transport to the shoot. ³ ⁶Cl⁻ fluxes across plasma membrane and tonoplast of K 51-40 and Paulsen roots showed that ³ ⁶Cl⁻ influx in root segments of Paulsen was greater than K 51-40 over the first 10 minutes. Unidirectional influx within 10 min loading time showed increases with increases in the external concentrations in both rootstocks but Paulsen had higher influx rate when compared to K 51-40. This appeared to be due to a greater Vmax. There was no significant difference in Km. It was shown that ³ ⁶Cl⁻ accumulation and transport rate to the shoot of K 51-40 was higher than that of Paulsen. Compartmental analysis of ³ ⁶Cl⁻ efflux from intact roots confirmed that the difference in influx observed between the rootstocks was consistent with the results obtained for excised roots, although the values were not exactly the same. It was also shown that the main root of Paulsen had greater contribution to ³ ⁶Cl⁻ uptake than lateral roots. ³ ⁶Cl⁻ fluxes by lateral roots were not significantly different between the rootstocks. Cl⁻ and Na⁺ distribution patterns in different root cell types were determined using the X-ray microanalysis technique. It was shown that Cl⁻ content in the hypodermis and cortical cells was higher than the other cell types in both rootstocks, but overall Cl⁻ content in the root of Paulsen was higher than K 51-40. The pericycle of the main root of Paulsen accumulated more Cl⁻ than K 51-40. It was concluded that Cl⁻ loading to the xylem was different in the rootstocks and Paulsen tended to prevent the xylem Cl⁻ loading process. Lateral roots also displayed opposite behaviour consistent with flux analysis. Membrane potential difference (PD) of the cortical cells showed a rapid and transient depolarization by adding 30 mM NaCl in both rootstocks that was followed by a gradual hyperpolarization. Depolarizations caused by 30 mM Choline-Cl, Na-MES and NaCl measured by the root surface potential method showed that Choline-Cl in K 51-40 and Na-MES in Paulsen caused greater depolarization than that of Na-MES in K 51-40 and Choline-Cl in Paulsen respectively. Assuming that PD measured in this method was the trans-root potential (TRP), it was concluded that the higher depolarization by Choline-Cl in K 51-40 can be due to higher Cl⁻ efflux rate to the xylem. Two different mechanisms were also detected for Cl⁻ transport: HATS which was observed in the range of 0.5-5 mM and a LATS in the range of 10-30 mM of the external NaCl concentration. This was consistent with the concentration dependence of Cl⁻ influx. In conclusion, evidence obtained from different experiments of this study indicated that in the grapevine rootstocks (Paulsen and K 51-40) Cl⁻ was mostly transported through the symplastic pathway. From ECl values determined for the rootstocks by the Nernst equation, a proton-driven transport system was responsible for Cl⁻ transport in both the HATS and LATS range of external NaCl concentrations. The rate of Cl⁻ transport from the root to shoot (xylem loading) was the major difference in Cl⁻ transport between the rootstocks in terms of salinity tolerance. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1339051 / Thesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food and Wine, 2008

In the present study, we investigated the mechanism of adenosine-induced apoptosis in Drosophila imaginal disc cell line using the overexpression and silencing of several candidate genes. Our results indicate that the cell death is associated with the activity of c-Jun N-terminal kinase (JNK).

Existem muitos tipos de substâncias orgânicas e inorgânicas que podem apresentar efeito tóxico ou inibidor aos organismos dos sistemas aeróbios de tratamento de efluentes, e entre essas substâncias está o cloreto. Nos efluentes, os cloretos se apresentam na forma de algum tipo de sal, e o mais comum é o cloreto de sódio. O cloreto de sódio pode ser encontrado em vários tipos de efluentes como, por exemplo, efluentes de indústrias alimentícias, petrolíferas, de processamento de couro e no lixiviado de aterros sanitários. Os cloretos podem além de prejudicar a microbiota, interferir no método de determinação da Demanda Química de Oxigênio - DQO e da Demanda Bioquímica de Oxigênio - DBO, que são parâmetros comumente utilizados para controle operacional de estações de tratamento. O presente trabalho teve como objetivo estudar a influência da adição de cloretos em um sistema de tratamento aeróbio por lodos ativados de bancada, que foi avaliado com base na remoção de matéria orgânica e no desenvolvimento e interação da microbiota envolvida. Foi avaliado o efeito tóxico da adição de concentrações de cloreto variando de 0 a 16 g\'CL POT. -\'/L. Os resultados confirmaram que há uma perda significativa de eficiência de remoção de matéria orgânica, principalmente quando a concentração de cloretos é superior a 4 g\'CL POT. -\'/L. Observou-se também que determinadas concentrações de cloretos são tóxicas aos organismos e prejudicam a estrutura dos flocos biológicos. Além disso, concluiu-se que a determinação da DQO pelo método 5220D do Standard Methods for Examination of Water and Wastewater sofre, em alguns casos, a interferência do cloreto e portanto pode não ser o melhor parâmetro para avaliação de eficiência dos sistemas de tratamento que operam sob essa condição. / There are many types of organic and inorganic substances that may have toxic effect on microorganisms of aerobic systems for wastewater treatment, and among these substances is the chloride. In the effluents, the chlorides are in the form of some type of salt, and most common is sodium chloride. Sodium chloride can be found in various types of effluents, for example, effluents from food industries, oil, leather processing and leachate from landfills. The chlorides can also affect the microbiota, interfering with the method of determining the Chemical Oxygen Demand - COD and the Biochemical Oxygen Demand - BOD, which are commonly used parameters for operational control of treatment plants. This work aimed to study the influence of the addition of chlorides in a system of aerobic activated sludge bench, which was evaluated based on the removal of organic matter and the development and interaction of microorganisms involved. We evaluated the toxic effect of adding chloride concentrations ranging 0-16 g\'CL POT. -\' /L. The results confirmed that there is a significant loss of removal efficiency of organic matter, especially when the chloride concentration is more than 4 g\'CL POT. -\'/L. It was also noted that certain concentrations of chloride are toxic to microorganisms and affect the structure of biological flocs. Furthemore, it was concluded that the determination of COD by 5220D method of Standard Methods for Examination of Water and Wastewater suffers, in some cases, interference of chloride and maybe not the better parameter for evaluating efficiency of treatment systems that operate under this condition.

Bien que les nanofils III-V soient reconnus comme des candidats prometteurs pour le développement de cellules solaires de nouvelle génération pour leurs propriétés optiques très attractives, l'amélioration des performances attendue par rapport à leurs homologues 2D n'a pas encore été démontrée. L’investigation à l’échelle nanométrique est essentielle pour comprendre l'origine de l'écart existant entre les prédictions théoriques et les démonstrations expérimentales. L’analyse des nanofils uniques devrait permettre d'élucider les facteurs limitants (liés par exemple aux propriétés électriques des jonctions p-n internes, à l'homogénéité fil-à-fil et aux éventuelles défaillances) et de proposer des solutions pour améliorer les performances des dispositifs photovoltaïques à nanofils.Cette thèse explore l’utilisation des techniques de caractérisation par faisceau d’électrons pour extraire les paramètres fondamentaux pour la conversion photovoltaïque afin d’optimiser les propriétés de nanofils III-V crus sur Si.L’étude à l’échelle nanométrique porte tout d’abord sur des nanofils de GaAs et AlGaAs avec une jonction radiale. A la suite de cette étude, la structure interne de nanofils a pu être améliorée. La caractérisation de dispositifs de taille millimétrique confirme l’amélioration des performances à l’échelle macroscopique.Des nanofils InGaP crus par une nouvelle méthode (Template Assisted Selective Epitaxy où TASE) ont aussi été étudiés et le niveau de dopage a été estimé par la microscopie EBIC. De plus, la réponse photovoltaïque de ces structures été observée pour la premier fois. Les propriétés électriques des nanofils GaAs avec une jonction axiale crus par la même technique ont aussi été caractérisées.Enfin, des nanofils avec deux jonctions InP/InGaP sont été étudiés comme première tentative pour fabriquer une cellule solaire tandem entièrement à nanofils. L’activité électrique des deux jonctions été observée et caractérisée. En revanche, le fonctionnement de la structure tandem s’est trouvé limité par la jonction tunnel qui connecte électriquement les deux jonctions. / Although III-V nanowires (NWs) are recognized as promising candidates for the development of new generation solar cells thanks to their very attractive optical properties, the expected performance improvement over their 2D counterparts has not yet been demonstrated. Nanoscale analyses by electron beam-based techniques (EBIC,CL) are expected to elucidate the limiting factors and to propose solutions for enhancing the performance of NW photovoltaic (PV) devices.This PhD thesis applies the electron beam probe techniques to get access to the key parameters governing the PV conversion at a single NW level in order to further optimize the properties of III-V NWs grown on Si.First, GaAs and AlGaAs NWs containing a radial junction are investigated at the nanoscale and their internal structure is optimized. The characterization of mm-sized devices confirms the improvement of the device performance at the macroscopic level.Then InGaP and GaAs NWs grown by a novel Template Assisted Selective Epitaxy (TASE) method containing an axial junction are studied. The doping level in the ternary alloy is estimated by EBIC and the photovoltaic response of these structures is demonstrated for the first time.Finally, InP/InGaP dual junction NWs are characterized. Although both top and bottom junctions are electrically active under excitation, the performance of the tandem structure is limited by the connecting tunnel junction

This thesis focus on the epitaxial growth and optical characterization of diluted InAs/InP(001) quantum dots for the realisation of new nanophotonic devices emitting at 1.55 [micro]m. The structural and optical properties of the quantum islands are correlated to different growth parameters of a solid source molecular beam epitaxy system. Our results highlight the influence of InAs surface reconstructions on the island shape. Dots rather than elongated dashes usually observed can be directly formed by adequate growth conditions. Dash to dot shape transition is also demonstrated by post-growth treatments. Low dot densities are obtained for small InAs deposited thickness. Their emission wavelength is easily tuned to 1.55 [micro]m using the"double cap" procedure for the growth of the InP capping layer. Optical properties of such single InAs/InP quantum dots are then evaluated. Micro-photoluminescence studies reveal sharp and well separated emission lines near 1.55 [micro]m from single dots confirming their atom-like properties. Last, we propose for the first time a high spatial resolution method to study the carrier transport in the vicinity of a single quantum dot using a low-voltage cathodoluminescence technique. A direct measurement of the carrier diffusion length before capture into one dot has been obtained. These results open the way to the integration of these single dots into optical micro-cavities for the realisation of quantum light sources at 1.55 [micro]m.

In order to effectively model the behaviour of energetic materials under operational conditions it is essential to obtain detailed structural information for these compounds at elevated temperature and/or pressures. The structural characterisation of the high explosives RDX and CL-20 and a series of inorganic azides [Mn+(N3)n] at extreme conditions is described herein. In addition to the characterisation of a highly metastable β -form of RDX (1,3,5- trinitrohexahydro-1,3,5-triazine) at atmospheric pressure, the structure solution of a high-pressure/ high-temperature polymorph is described. This form, obtained above 4.3 GPa and 450 K, has been shown to be distinct from the β -form and has therefore been denoted - RDX. Furthermore, ε -RDX is sufficiently metastable to allow its recovery to ambient pressure at 150 K; it only transforms to the α -form upon warming to 230 K. Finally, the ambient-temperature compression of RDX has been investigated to a maximum pressure of 23.0 GPa, using methanol:ethanol (4:1) as the pressure-transmitting medium; no phase transition was observed under these conditions, other than the α → γ transition at 3.9 GPa. The structure of a high-pressure polymorph of CL-20 (2,4,6,8,10,12- hexanitrohexaazaisowurtzitane) has also been determined by a combination of powder and single-crystal X-ray diffraction. Compression of γ -CL-20 to above 0.7 GPa using Fluorinert (FC-77) as the pressure-transmitting medium results in a phase transition to the ζ -form, which has been found to display structural similarities with both theγ γ - and ε -forms. The high-pressure behaviour of CL-20, however, depends markedly on the starting polymorph and the pressure-transmitting medium selected. Compression of γ -CL-20 in MeOH:EtOH (4:1) results in the formation of a 2:1 CL-20:MeOH solvate at 0.5 GPa. This solvate is stable upon compression to P > 5.0 GPa. It may also be recovered to ambient pressure at 293 K. Meanwhile, no phase transition is observed during the compression of ε -CL-20 to a maximum pressure of 7.2 GPa. Finally, a series of inorganic azides [NaN3, CsN3, TlN3, NH4N3, AgN3 and Pb(N3)2] has been characterised under a range of pressure and temperature conditions. Of the six compounds studied, all displayed at least one polymorphic transition – 5 new forms have been structurally characterised in this work and evidence of another 5 is presented. The combined effect of pressure and temperature results in sodium azide adopting a tetragonal structure common to larger alkali metal azides. Caesium azide has been shown to undergo three phase transitions during compression to 6.0 GPa – the structure of the first high-pressure form is reported. A variable temperature X-ray powder diffraction study of TlN3 has allowed the structural characterisation of the low-temperature TlN3-IV (at 230 K) as well as providing evidence for a phase transition to a high-temperature form above 550 K. The high-pressure form III (obtained above 0.76 GPa) has also been determined by neutron powder diffraction. Silver, ammonium and lead(II) azides have all been shown to undergo a phase transition at high pressures. Compression of silver azide (P > 0.80 GPa) removes an orthorhombic distortion observed at atmospheric pressure, resulting in the tetragonal structure adopted by CsN3 and TlN3 under ambient conditions. Moreover, NH4N3 and Pb(N3)2 have been found to undergo phase transitions at 2.6 GPa, although their high-pressure structures have still to be determined.

O presente estudo foi realizado com o objetivo de se desenvolver e validar uma metodologia analítica indicativa de estabilidade para separação e quantificação de anlodipino e seus produtos de degradação no medicamento besilato de anlodipino 5 mg por comprimido. Para tanto, realizou-se um estudo de degradação forçada, como forma de identificar os principais produtos de degradação, que podem vir a formar num estudo de estabilidade, bem como estabelecer possíveis rotas de degradação. Foi utilizada a técnica instrumental de separação, cromatografia a líquido (CL), com dois tipos de detectores: arranjo de diodos (DAD) e corona CAD (Detector de aerossol carregado), coluna cromatográfica Zorbax SB-Phenyl 1,8µm 4,6 x 50 mm Agilent® e fase móvel constituída por tampão pH3,0, metanol e acetonitrila sob condições de gradiente (75% de tampão pH 3,0, 15% de metanol e 10% de acetonitrila; no tempo de 10 minutos tem-se 20% de tampão pH3,0, 60% de metanol e 20% de acetonitrila; no tempo de 13 minutos tem-se 75% de tampão pH3,0, 15% de metanol e 10% de acetonitrila; e no tempo de 15 minutos tem-se 75% de tampão pH3,0, 15% de metanol e 10% de acetonitrila); fluxo de 1,0mL/min até 10 minutos; de 10,1 a 13 minutos tem-se fluxo de 0,5 mL/min; e de 13,1 a 15 minutos volta ao fluxo de 1,0 mL/min; e detecção em 238 nm. O método apresentou-se linear, preciso, exato, robusto e seletivo, e proporcionou resultados confiáveis, sem interferência de degradantes e impurezas. / This study was conducted in order to develop an analytical methodology indicative of stability for separation and quantification of the antihypertensive amlodipine and its degradation products in the drug amlodipine besylate 5 mg per tablet. For this development was performed a forced degradation study, in order to identify the main degradation products that may form in a stability study, as well as establish possible degradation routes. It was used the instrumental separation technique, liquid chromatography (LC) with two types of detectors: diode array (DAD) and corona CAD (Charged Aerosol Detector), chromatographic column Zorbax SB-Phenyl 4.6 x 50 mm 1,8µm Agilent® and mobile phase of buffer pH3,0, methanol and acetonitrile under gradient conditions (75% buffer pH3,0, 15% methanol and 10% acetonitrile; 10 minutes has 20% buffer pH3,0, 60% methanol and 20% acetonitrile; 13 minutes has become 75% of buffer pH3,0, 15% methanol and 10% acetonitrile; and 15 minutes has become 75% of buffer pH3,0, 15% methanol and 10% acetonitrile); flow 1.0 mL/ min up to 10 minutes; a flow 0,5 mL/min is obtained from 10,1 to 13 minutes; and from 13,1 minutes the flows returns to 1,0 mL/min; and detection in 238 nm. The method is linear, precise, accurate, robust and selective, and provided reliable results without interference from degradation products and impurities.

Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-03-23T14:53:53Z No. of bitstreams: 1 DIS_ELUSA_SCHEIBLER_PARCIAL.pdf: 424780 bytes, checksum: 1d777a8f417e06f635286ca4d52e7d71 (MD5) / Made available in DSpace on 2017-03-23T14:53:54Z (GMT). No. of bitstreams: 1 DIS_ELUSA_SCHEIBLER_PARCIAL.pdf: 424780 bytes, checksum: 1d777a8f417e06f635286ca4d52e7d71 (MD5) Previous issue date: 2017-01-13 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Candidiasis is the most prevalent fungal infection in the oral cavity of human beings. It is an oportunistic disease, which has predisposing factors such as xerostomia, alcohol and tobacco use, ill-fitted and poor cleaned dentures, diabetes mellitus, severe nutritional deficiencies and imunosuppressive conditions such as HIV infection and chemotherapy. Nystatin and chlorhexidine have been widely used to control oral infections, sometimes in an associated protocol. Nevertheless, there are reports about an antagonistic interaction when these drugs are combined. The present work aimed to analyze, in vitro, the effect of combined nystatin and chlorhexidine on Candida albicansand the stability of their mixture as well. The drugs were tested alone and in different combinations including single formulation and independent formulations with different time intervals. The minimum inhibitory concentration (MIC) of the drugs was determined by the broth microdilution method, and also their effects on the optical density (OD) of suspensions and biofilms of C. albicans were evaluated.The stability of nystatin/chorhexidine mixture was tested in high performance liquid chromatography (HPLC). Nystatin/chlorhexidine single formulation did not show a significantly differentMICcompared to the drugs used alone.The combinations with time intervalsbetween drugs showed higher MIC than the drugs alone, except for the MIC of chlorhexidine with 10-min interval. According to our results, the combination of the drugs interferes with theMIC, increasing its level, exceptfor the chlorhexidine MIC in nystatin/chlorhexidine single formulation. Nystatin/chlorhexidine combinations showed impaired activity against C.albicans biofilms. Among the combinations, that with 30-min interval showed the highest antifungal effect and the single formulation the lowest. The drugs when combined showed high percentages of degradation at all the times evaluated. Conclusion: The combination of nystatin and chlorhexidine interferes with their effectiveness and also increases their rate of degradation. / A candid?ase ? a infec??o f?ngica mais prevalente na cavidade oral de seres humanos. ? uma infec??o oportunista, que tem por fatores predisponentes a xerostomia, o uso de ?lcool e tabaco, pr?teses mal-higienizadas e mal-adptadas, diabetes mellitus, defici?ncias nutricionais graves e condi??es imunossupressoras como infec??o pelo HIV e quimioterapia. A nistatina e a clorexidina t?m sido amplamente empregadas no controle de processos infecciosos da cavidade oral, algumas vezes simultaneamente. Entretanto, h? relatos na literatura de que, se empregadas de forma combinada, essas drogas teriam intera??o antag?nica. O presente estudoteve por objetivo avaliar, in vitro, o efeito do uso concomitante de clorexidina e nistatina sobre Candida albicans. As drogas foram testadas em diferentes combina??es, incluindo formula??o ?nica e formula??es independentes empregadas de formaassociadacom diferentes intervalos de tempo. Os testes foram realizados determinando-se a concentra??o inibit?ria m?nima (CIM) por meio do m?todo de microdilui??o em caldo,bemcomo avalia??o do efeito sobre a densidade ?ptica das suspens?es e biofilmes de C. albicans. A estabilidade da mistura de nistatina e clorexidina foi testada por meio de cromatografia l?quida de alta efici?ncia (HPLC). A formula??o ?nica nistatina/clorexidina n?o exibiu CIM significativamente diferente dos respectivos f?rmacos empregados isoladamente. As combina??es com intervalos de tempo intermedi?riostiveram CIM significativamente maiordo que o f?rmaco isolado, exceto para a CIM da clorexidina ao intervalo de 10 min. De acordo com esses resultados, a combina??o dos f?rmacos interfere nasrespectivas CIMs, elevando-as, com exce??o da CIM da clorexidina para formula??o ?nica. Comparadas ao uso das drogas isoladas, as combina??es de nistatina e clorexidina exibiram atividade diminu?da nos biofilmes de C.albicans,tendo o intervalo de 30 minutos o maior efeito antif?ngico, ea formula??o ?nica, o menor. A mistura das drogas teve elevadas percentagens de degrada??o em todos os tempos avaliados. Conclus?o: A combina??o de nistatina e clorexidina interfere na efetividadedas drogas e tamb?m eleva as suas taxas de degrada??o.

Submitted by PPG Psicologia (psicologia-pg@pucrs.br) on 2018-04-04T20:40:55Z No. of bitstreams: 1 DIS_JORGE_ONDERE_NETO.pdf: 1302917 bytes, checksum: e482081ae12fd104dbef1296a6375aff (MD5) / Rejected by Caroline Xavier (caroline.xavier@pucrs.br), reason: Devolvido devido a diverg?ncia na data de defesa da disserta??o. on 2018-04-20T18:04:44Z (GMT) / Submitted by PPG Psicologia (psicologia-pg@pucrs.br) on 2018-04-20T18:33:54Z No. of bitstreams: 1 JORGE_ONDERE_NETO_DIS.pdf: 2134446 bytes, checksum: 970b77376b66c486039e25b14cb56fe2 (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2018-04-20T18:42:56Z (GMT) No. of bitstreams: 1 JORGE_ONDERE_NETO_DIS.pdf: 2134446 bytes, checksum: 970b77376b66c486039e25b14cb56fe2 (MD5) / Made available in DSpace on 2018-04-20T18:48:16Z (GMT). No. of bitstreams: 1 JORGE_ONDERE_NETO_DIS.pdf: 2134446 bytes, checksum: 970b77376b66c486039e25b14cb56fe2 (MD5) Previous issue date: 2018-03-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Introduction: The Outcome Questionnaire System (OQ System) is a system developed by the American psychologists Michael Lambert and Gary Burlingame in the early 90s; nowadays it is recognized by the Substance Abuse and Mental Health Administration's National Registry of Evidence-based Programs and Practices (SAMHSA's NREPP). The system is composed by questionnaires for the purpose of obtaining quantitative data on psychotherapeutic treatment in order to evaluate and monitor its outcome (Erekson, Lambert & Eggett, 2015). The results obtained through the application of the questionnaires in the context of psychotherapy helped to verify if the intervention is effective so that, through this feedback, the psychotherapist can prevent withdrawal, modify the therapeutic plan and provide feedback to the patient and the team in order to discuss the clinical case based on quantitative evidence (Nordal, 2012). Among the several questionnaires in the OQ System, the Youth Outcome Questionnaire Self-Report 2.0 (Y-OQ-SR 2.0) was developed to evaluate the psychotherapy of teenagers between 12 and 18 years old (Wells & Burlingame, 2003). The Y-OQ SR 2.0 has 64 items consisting of affirmative first-person phrases and shall be answered on a Likert scale from 0 to 4: 0 (never), 1 (rarely), 2 (sometimes), 3 (often ) and 4 (always). The items are distributed into six factors that evaluate: Intrapersonal Stress; Somatic; Interpersonal Relationships; Critical Items; Social problems; Behavioral Disorders. The questionnaire is non-theoretical and self-applied. Goal: culturally adapt the Y-OQ-SR 2.0 to the Brazilian reality. Method: the study followed the following steps: 1) From the original instrument, two translations into Portuguese were performed by two bilingual translators; 2) Synthesis of the two translations by a third translator overseen by an expert committee; 3) Evaluation of the target audience in order to investigate the understanding of the items; 4) Adjusting the instrument after a consensus among the ideas resulting from the evaluation of the target audience; 5) Back-translation of the Portuguese version into English, by a fourth bilingual translator; 6) Submission of the back-translation to the original authors to ensure that the content has equivalent meaning to the original content; 7) The original authors considered the back-translation appropriate, the Portuguese translation will be used to carry out a pilot study (Borsa et al., 2012; Cassepp-Borges, Balbinotti & Teodoro, 2010; Gjersing, Caplehorn & Clausen, 2010; Takara, 2015). Outcomes: Some terms and words that needed clarification were evaluated by a committee of experts before the synthesis itself. The translation was made by two bilingual translators using the Parallel Blind Technique. The synthesis of the translations was evaluated by the target audience in order to ensure the understanding of the translated items. Five out of these items required review by the committee for a second audience assessment, followed by semantic adjustments. This second evaluation took place satisfactorily, as the target audience understood the phrases and modified words. The final version was submitted to the reverse translation process (Portuguese to English) by a C2 level translator in the Common European Framework of Reference (CEFR). Finally, the final version was sent to the original authors in order to ensure the equivalence of content with the original version. The final approval of the Brazilian-Portuguese version of the instrument was satisfactory; in other words, no adjustments were required. A committee of experts comprehending adolescent clinical psychologists with fluency in English employed the procedures for operational equivalence and it was concluded that the Y-OQ-SR 2.0 is suitable for the pilot study. Discussion: The evaluation of the target audience was made through stratified focus groups. The group provided discussions about the items to be evaluated, providing both objective data, that were filled by adolescents, and subjective data related to participants' understanding and discussion on terms and words. Once the steps proposed by Borsa et al., 2012, Cassepp-Borges, Balbinotti & Teodoro, 2010, Gjersing, Caplehorn & Clausen, 2010 and Takara, 2015 were successfully achieved, the proposed goal was therefore achieved. The gathering and analysis of data were performed through a rigorous and systematic process to ensure adequate adaptation to the Brazilian reality. The Y-OQ-SR 2.0 is an important tool for adolescent and patient psychotherapists, as it was developed exclusively to assess youth psychotherapy. Its purpose is, therefore, to monitor the psychotherapeutic process (Lambert, 2010) and provide feedback to find out if the psychotherapy is being effective (Lambert, Hansen & Harmon, 2010). The adaptation of the Y-OQ-SR 2.0 to the Brazilian reality will allow the instrument to be released for subsequent studies of evidence of validity. / O Outcome Questionnaire System (OQ System) ? um sistema desenvolvido pelos psic?logos americanos Michael Lambert e Gary Burlingame no in?cio da d?cada de 90 e, atualmente, ? reconhecido no Substance Abuse and Mental Health Administration`s National Registry of Evidence-based Programs and Practices (SAMHSA`sNREPP). O sistema ? composto por question?rios destinados a obter dados quantitativos do tratamento psicoterap?utico com o objetivo de avaliar e monitorar o seu desfecho (Erekson, Lambert & Eggett, 2015). Os resultados obtidos por meio da aplica??o dos question?rios no contexto da psicoterapia auxiliam a verificar se a interven??o est? sendo tanto eficaz quanto efetiva e, por meio desse feedback, o psicoterapeuta pode prevenir desist?ncias, modificar o plano terap?utico e fornecer retorno ao paciente e ? equipe de maneira a discutir o caso cl?nico baseado em evid?ncias quantitativas (Nordal, 2012). Dentre os diferentes question?rios inclu?dos no OQ System, o Youth Outcome Questionnaire Self-Report 2.0 (Y-OQ-SR 2.0) foi desenvolvido para avaliar a psicoterapia de adolescentes entre 12 e 18 anos (Wells & Burlingame, 2003). O Y-OQ SR 2.0 possui 64 itens que consistem em frases afirmativas em primeira pessoa para serem respondidos por meio de uma escala Likert de 0 a 4: 0 (nunca), 1 (raramente), 2 (?s vezes), 3 (frequentemente) e 4 (sempre). Os itens est?o divididos em seis fatores que avaliam: Estresse Intrapessoal; Som?tico; Rela??es Interpessoais; Itens Cr?ticos; Problemas Sociais; Transtornos Comportamentais. O question?rio ? ate?rico e autoaplic?vel. Objetivo: adaptar culturalmente o Y-OQ-SR 2.0 para a realidade brasileira. M?todo: o estudo seguiu as seguintes etapas: 1) A partir do instrumento original, duas tradu??es para o portugu?s foram realizadas por dois tradutores bil?ngues; 2) S?ntese das duas tradu??es por um terceiro tradutor com acompanhamento de um comit? de experts; 3) Avalia??o do p?blico-alvo com objetivo de investigar a compreens?o dos itens; 4) Ajuste do instrumento ap?s um consenso entre as ideias advindas da avalia??o do p?blico-alvo; 5) Retrotradu??o da vers?o em portugu?s para o ingl?s, realizada por um quarto tradutor bil?ngue; 6) Envio da retrotradu??o (vers?o em ingl?s) para os autores originais para assegurar que o conte?do tenha significado equivalente ao conte?do original; 7) Os autores originais consideraram a retrotradu??o adequada, a tradu??o em portugu?s ser? utilizada para a execu??o de um estudo-piloto (Borsa et al., 2012; Cassepp-Borges, Balbinotti & Teodoro, 2010; Gjersing, Caplehorn & Clausen, 2010; Takara, 2015). Resultados: Alguns termos e palavras que necessitaram de esclarecimentos foram avaliados por um comit? de experts antes da s?ntese propriamente dita. A tradu??o foi feita por dois tradutores bil?ngues utilizando a T?cnica Cega Paralela (Parallel Blind Technique). A s?ntese das tradu??es foi avaliada pelo p?blico-alvo para averiguar a compreens?o dos itens traduzidos. Destes itens, cinco necessitaram de revis?o pelo comit? para uma segunda avalia??o do p?blico-alvo seguida de ajustes sem?nticos. Esta segunda avalia??o ocorreu de maneira satisfat?ria, pois o p?blico-alvo compreendeu as frases e palavras modificadas. A vers?o final foi submetida ao processo de tradu??o reversa (portugu?s para o ingl?s) por um tradutor n?vel C2 no Quadro Europeu Comum de Refer?ncia (QECR). Por fim, a vers?o final foi encaminhada aos autores originas com objetivo de assegurar a equival?ncia de conte?do com a vers?o original. A aprova??o final da vers?o para o portugu?s brasileiro do instrumento ocorreu de maneira satisfat?ria, ou seja, n?o foram necess?rios ajustes. Um comit? de experts composto por psic?logos cl?nicos que atuam no atendimento de adolescentes e que possuem flu?ncia em ingl?s empregou os procedimentos para a equival?ncia operacional e foi conclu?do que o Y-OQ-SR 2.0 est? adequado para o estudo-piloto. Discuss?o: a avalia??o do publico-alvo foi feita por meio de grupos focais estratificados. O grupo propiciou discuss?es acerca dos itens a serem avaliados fornecendo dados tanto objetivos que foram preenchidos pelos adolescentes quanto subjetivos relacionados ao entendimento e discuss?o dos participantes acerca dos termos e das palavras. Uma vez que as etapas propostas por Borsa et al., 2012, Cassepp-Borges, Balbinotti & Teodoro, 2010, Gjersing, Caplehorn & Clausen, 2010 e Takara, 2015 foram alcan?adas com ?xito, o objetivo proposto foi, portanto, alcan?ado. As coletas bem como as analyses dos dados ocorreram a partir de um processo rigoroso e sistem?tico de modo a garantir adequada adapta??o para a realidade brasileira. O Y-OQ-SR 2.0 ? um instrumento importante para psicoterapeutas de adolescentes e pacientes, pois ele foi desenvolvido exclusivamente para avaliar a psicoterapia do p?blico juvenil. Sua finalidade ?, portanto, monitorar o processo psicoterap?utico (Lambert, 2010) e fornecer feedbacks para verificar se a psicoterapia est? sendo eficiente (Lambert, Hansen & Harmon, 2010). A adapta??o do Y-OQ-SR 2.0 para a realidade brasileira ir? propiciar que o instrumento seja liberado para estudos subsequentes de evid?ncias de validade.