The contamination of seafood with methylmercury (MeHg) is a global health issue, as MeHg is a well known neurotoxin. Since dietary nutrients may interact with MeHg toxicity, and oxidative stress is one of the primary mechanisms underlying MeHg neurotoxicity, we characterized dietary antioxidant-MeHg interactions. Firstly, we used an ethnobotanical study to confirm the antioxidant activity of Northern Labrador Tea, Rhododendron tomentosum ssp. subarcticum (Tea), for the Canadian Inuit, a population with elevated MeHg exposure. Secondly, we determined the ability of Tea to ameliorate MeHg-induced toxicity in a rat perinatal exposure study. MeHg exposure (2 mg/KgBW/d) was associated with perturbed development and behaviour, elevated brain N-methyl-D-aspartate receptors, and serum lipid peroxidation. Surprisingly, Tea co-exposure (100 mg/KgBW/d) modulated MeHg’s effects on brain NMDA-R levels and lipid peroxidation, but also increased mercury serum concentrations. Thirdly, using a toxicogenomics approach we determined that MeHg exposure caused the down-regulation of Nr4a2 and its protein product Nurr1. These novel MeHg targets are implicated in developmental learning functions and were corrected with MeHg + Tea co-exposure. Lastly, we conducted a risk assessment survey and cross-sectional dietary epidemiology study in Costa Rica to further investigate dietary nutrient-MeHg interactions. Costa Rica is a Central American country with multiple sources of Hg and a high per capital fish consumption. Here, 5 of the 14 populations we studied exceeded the recommended MeHg provisional tolerable daily intake (pTDI) of 0.2 µg/KgBW/d. In Heredia the pTDI was exceeded by 34% of woman participants, primarily associated with canned tuna consumption. Interestingly, we detected that Hg body burden was significantly reduced by the consumption of antioxidant-rich dietary items. Considering our collective results, we hypothesized that MeHg toxicokinetics may be altered by dietary nutrients at the site of intestinal absorption from the disruption of gut flora, or at the site of cellular demethylation in tissues from the improvement of cellular redox state. The interaction of dietary nutrients on MeHg outcomes has a large impact on risk assessment and may provide a public health approach for managing the risk associated with MeHg exposure without reducing local fish consumption.

<p>Despite being distributed worldwide and easily collected, the biology, ecology, and taxonomy of Ptiliidae Heer, 1843, or featherwing beetles, have not been well studied. In a study from 2007 to 2009, Ptiliidae were extracted from various polypore fungi collected throughout Costa Rica in an effort to expand biogeographic knowledge of Ptiliidae. Fungi and Ptiliidae were identified to genera and collection sites mapped. Beetle genera are able to inhabit different polypore genera and were found at a higher rate of co-occurrence than reported in previous studies. We identified <i>Cylindrosella costariciensis </i><b>sp. n.</b>, with the potential of two more new species to be described.</p>

The oceans are essential for humanity, yet these systems continue to be degraded and suffer from pollution, habitat destruction and overexploitation. The Costa Rican Gulf of Nicoya is a productive gulf that is fished by both large-scale industrial fisheries and small-scale artisanal fishermen. However, the gulf is profoundly overfished and there has been a shift in both the type of species and size of fishes being targeted, which has had devastating economic and ecological effects in the region. To improve their livelihoods, artisanal fishermen have together with governmental agencies instituted partially protected marine protected areas, known as AMPRs which are intended to give artisanal fishermen exclusive fishing rights and to better the health of the ecosystems. However, these AMPRs can vary greatly within and between each other in aspects such as what types of fishing gear is allowed. To investigate how fishing techniques impact the health of the fish stocks and the wellbeing of fishermen within an AMPR, the size of a target fish, Spotted Rose Snapper (Lutjanus guttatus) and the number of undesired fish caught, was compared based on the number of approved fishing methods such as nets, longlines and commercial scuba diving, between different zones. This was conducted by identifying and measuring catch in collaboration with the National University of Costa Rica, who contributed with databases and field assistance, and artisanal dead bait longline fishermen (bottom long line) in the Paquera-Tambor AMPR. The results showed that in areas where fishing gear is more restricted, the mean weight of Spotted Rose Snapper was higher, but the relative proportion bycatch was not different. Furthermore, there was no difference in legal capture or breeding lengths between areas with high and low restriction of fishing gear. This indicates that the effects of overfishing are less predominant in zones where fewer fishing methods are permitted. The restriction of fishing methods could increase biodiversity and size in fish by exploiting less cohorts of populations. The preservation of size in fish species is very important regarding reproductive success, and it is therefore paramount that larger individuals are protected. This could be accomplished by for example, restricting the amount of allowed fishing gear or introducing maximum catch sizes. However, an improvement of the fish stocks, and therefore human wellbeing cannot be achieved in the Gulf of Nicoya without a revision of the strategies of two influential governmental bodies, the Costa Rican coast guard and the Costa Rican Institute of Fisheries and Aquaculture.

This thesis covers the methods and the goals of organizations located in Costa Rica working for the good of Nicaraguan citizens. Through a thematic analysis of 10 Facebook posts per 8 civil society organizations, four different themes within the posts were found: active action, cooperation, informing, and political speech. These themes as well as different extracts from the posts have together given a picture of the methods and goals of the organizations. Active action, cooperation and informing are used to see the methods of the organizations, while political speech and some cases of informing show the goals of the organizations. Through the extracts there are examples of what they do and why they do it. What has been found is that the organizations are different from each other in some respects. The organizations with a focus on Nicaraguans who are in Costa Rica have a higher count of posts using active action while those focusing on the situation in Nicaragua use more political speech. It has also been found that theories regarding the development of social movements, activism and peaceful disobedience can be used to understand the actions and the goals of the organizations.

Like other countries of the world, Costa Rica faced the challenge of dealing with a variety of trade-offs when implementing sustainability goals in agriculture. Very often, economic promotion is in conflict with goals regarding human and environmental health protection. Organic farming practices could provide strategies to overcome some of these trade-offs. However, in Costa Rica, the majority of farmers still relies on conventional farm practices. In this paper, I investigate the potential for a sustainable transformation in Costa Rica’s agriculture by focusing on organic farming policies. I shed light on the role local actors and organizations play in this process compared to other actor types. I argue that local actors are “the agents of change” in these processes, as these are the target groups of organic farming policies and are the ones who are asked to change their farm practices. Based on survey data and network analysis, I was able to illustrate how differently integrated local actors are compared to other actor types in Costa Rica’s implementation of organic farming policies. Local actors show interest and willingness to further participate in land-use implementation processes when institutional barriers are alleviated, and further promotion instruments are available.

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Two-thirds of submarine volcanism in the Earth's ocean basins is manifested along mid-ocean ridges and the remaining one-third is revealed along intraoceanic arcs and seamounts. Hydrothermal systems and the circulation patterns associated with these volcanic settings remove heat from the solid Earth into the deep ocean. Hydrothermal circulation continues to remove and redistribute heat in the crust as it ages. The heat and mass fluxes added to the deep ocean influence mixing in the abyssal ocean thereby affecting global thermohaline circulation. In addition to removing heat, hydrothermal processes extract chemical components from the oceanic and carry it to the surface of the ocean floor, while also removing certain elements from seawater. The resulting geochemical cycling has ramifications on the localized mineral deposits and also the biota that utilize these chemical fluxes as nutrients. In this dissertation, I analyze observed conductive heat flow measurements in the Panama Basin and borehole thermal measurements in Brothers Volcano and use mathematical models to estimate advective heat and mass fluxes, and crustal permeability. In the first manuscript, I use a well-mixed aquifer model to explain the heat transport in a sediment pond in the inactive part of the Ecuador Fracture Zone. This model yields mass fluxes and permeabilities similar to estimates at young upper oceanic crust suggesting vigorous convection beneath the sediment layer. In the second manuscript, I analyze the conductive heat flow measurements made in oceanic between 1.5 and 5.7 Ma on the southern flank of the Costa Rica Rift. These data show a mean conductive heat deficit of 70%, and this deficit is explained by various hydrothermal advective transport mechanisms, including outcrop to outcrop circulation, transport through faults, and redistribution of heat by flow of hydrothermal fluids in the basement. In the third manuscript, I analyze the borehole temperature logs for two sites representative of recharge and discharge areas of hydrothermal systems in the Brothers Volcano. I develop upflow and downflow models for fluids in the borehole and formation resulting in estimated of flow rates and permeabilities. All three independent research works are connected by the common thread of utilizing relatively simple mathematical concepts to get new insights into hydrothermal processes in oceanic crust. / PHD / Two-thirds of underwater volcanic activity in the Earth’s ocean basins is exhibited in areas where new material for Earth’s outer shell is created and the remaining one-third is displayed along areas where the outer shell is destroyed. In these areas, hot springs that are under water and their water movement patterns remove heat from the solid outer shell and puts it into the deepest parts of the ocean. Hot water circulation continues to remove and redistribute heat and various chemical elements in the shell as it grows old. This heat and chemical elements, which get added to the deep ocean water, influences the way water mixes and forms layers in the world oceans. This also affects the movement of ocean currents. The chemical elements removed from the shell by hot water gets deposited as minerals on the ocean floor in places where hot springs arise. This variety of minerals provides nutrients for different marine organisms. In this work done during my PhD studies, I examine the heat and temperature that was measured in the Panama Basin and Brothers Volcano. I utilize these examinations to build simple math models to find out how much heat and chemical components are being added to the deep ocean water. I also find out the methods in which the hot water springs appear on the ocean floor and the patterns in which the hot water circulates in the Earth’s outer shell. All of these estimates will help the scientists who are studying the patterns and changes in ocean currents by giving them a number on how much heat is released from the inside of the Earth.