Durante décadas, a visão prevalente em ecologia de microrganismos era de que os fatores ambientais locais seriam os únicos agentes estruturantes das comunidades e dos padrões de coexistência das espécies. Entretando, estudos recentes têm fornecido evidência de que processos ligados a dispersão possam ser tão importantes quanto o ambiente local na estruturação das comunidades, reacendendo o debate em torno da hipótese clássica sobre a ubiquidade dos microrganismos. Neste estudo, nós examinamos os padrões de coexistência do fitoplâncton e os possíveis determinantes da variação espacial na composição das comunidades em 9 lagoas do sul do Brasil. Nós testamos se as espécies fitoplanctônicas apresentam um padrão não aleatório de coexistência através de um modelo nulo e se distância espacial ou a distância ambiental possuem relação com a similaridade na composição das comunidades. Nós também investigamos se as variáveis ambientais locais são bons preditores da abundância das espécies. Na análise de coexistência, nós não observamos um padrão significativo de estruturação dentro das lagoas, mas encontramos um padrão não-aleatório e segregado das espécies ao longo das lagoas, indicando que as espécies coexistem menos do que o esperado ao acaso nesta escala. A similaridade na composição das comunidades mostrou correlação significativa com a distância ambiental, mas não foi correlacionada com a distância geográfica entre os sítios. Além disso, a variação na abundância das espécies apresentou correlação significativa com as variáveis ambientais locais (transparência da água, condutividade, nitrato e ortofosfato). Em conclusão, nossos resultados apoiam a visão de que a variação espacial do fitoplâncton é melhor explicada pelo ambiente local. Nós não testamos a influência de variáveis históricas e filogenéticas sobre esses padrões, que podem ser temas de estudos futuros, a fim de elucidar ainda mais essas questões. / For decades, the main hypothesis in microbial ecology was that local environment would be the only mechanism structuring communities and patterns of species coexistence. However, recent studies have provided evidence that processes related to dispersion can be as important as the local environment for shaping biological communities, stimulating new debates on the classical hypothesis about the ubiquity of microorganisms. In this study, we examined the phytoplankton coexistence patterns and possible determinants of spatial variation in community composition in 9 lakes of southern Brazil. First, we use a null model analysis for tested whether the phytoplankton species show a nonrandom pattern of coexistence. We also tested whether spatial distance or environmental distance act as potential factors controlling community composition. Finally, we investigated whether local environmental variables are good predictors of species abundance. We did not observe a significant pattern of coexistence species in the within-lakes analysis, but we found a nonrandom pattern of coexistence and segregation of species across-lakes analysis, indicating that the species coexist less than expected by chance on this scale. Phytoplankton community composition showed a strong positive correlation with the environmental distance but was not correlated with the geographical distance between sites. Moreover, the variation in the abundance of species showed significant correlation with local environmental variables (water transparency, conductivity, nitrate and orthophosphate). In conclusion, our results support the view that the spatial variation of phytoplankton is best explained by the local environmental conditions. We suggest that the influences of historical and phylogenetic variables on these patterns, here not measured, be analyzed in future studies, to further elucidate these questions.

Durante décadas, a visão prevalente em ecologia de microrganismos era de que os fatores ambientais locais seriam os únicos agentes estruturantes das comunidades e dos padrões de coexistência das espécies. Entretando, estudos recentes têm fornecido evidência de que processos ligados a dispersão possam ser tão importantes quanto o ambiente local na estruturação das comunidades, reacendendo o debate em torno da hipótese clássica sobre a ubiquidade dos microrganismos. Neste estudo, nós examinamos os padrões de coexistência do fitoplâncton e os possíveis determinantes da variação espacial na composição das comunidades em 9 lagoas do sul do Brasil. Nós testamos se as espécies fitoplanctônicas apresentam um padrão não aleatório de coexistência através de um modelo nulo e se distância espacial ou a distância ambiental possuem relação com a similaridade na composição das comunidades. Nós também investigamos se as variáveis ambientais locais são bons preditores da abundância das espécies. Na análise de coexistência, nós não observamos um padrão significativo de estruturação dentro das lagoas, mas encontramos um padrão não-aleatório e segregado das espécies ao longo das lagoas, indicando que as espécies coexistem menos do que o esperado ao acaso nesta escala. A similaridade na composição das comunidades mostrou correlação significativa com a distância ambiental, mas não foi correlacionada com a distância geográfica entre os sítios. Além disso, a variação na abundância das espécies apresentou correlação significativa com as variáveis ambientais locais (transparência da água, condutividade, nitrato e ortofosfato). Em conclusão, nossos resultados apoiam a visão de que a variação espacial do fitoplâncton é melhor explicada pelo ambiente local. Nós não testamos a influência de variáveis históricas e filogenéticas sobre esses padrões, que podem ser temas de estudos futuros, a fim de elucidar ainda mais essas questões. / For decades, the main hypothesis in microbial ecology was that local environment would be the only mechanism structuring communities and patterns of species coexistence. However, recent studies have provided evidence that processes related to dispersion can be as important as the local environment for shaping biological communities, stimulating new debates on the classical hypothesis about the ubiquity of microorganisms. In this study, we examined the phytoplankton coexistence patterns and possible determinants of spatial variation in community composition in 9 lakes of southern Brazil. First, we use a null model analysis for tested whether the phytoplankton species show a nonrandom pattern of coexistence. We also tested whether spatial distance or environmental distance act as potential factors controlling community composition. Finally, we investigated whether local environmental variables are good predictors of species abundance. We did not observe a significant pattern of coexistence species in the within-lakes analysis, but we found a nonrandom pattern of coexistence and segregation of species across-lakes analysis, indicating that the species coexist less than expected by chance on this scale. Phytoplankton community composition showed a strong positive correlation with the environmental distance but was not correlated with the geographical distance between sites. Moreover, the variation in the abundance of species showed significant correlation with local environmental variables (water transparency, conductivity, nitrate and orthophosphate). In conclusion, our results support the view that the spatial variation of phytoplankton is best explained by the local environmental conditions. We suggest that the influences of historical and phylogenetic variables on these patterns, here not measured, be analyzed in future studies, to further elucidate these questions.

Durante décadas, a visão prevalente em ecologia de microrganismos era de que os fatores ambientais locais seriam os únicos agentes estruturantes das comunidades e dos padrões de coexistência das espécies. Entretando, estudos recentes têm fornecido evidência de que processos ligados a dispersão possam ser tão importantes quanto o ambiente local na estruturação das comunidades, reacendendo o debate em torno da hipótese clássica sobre a ubiquidade dos microrganismos. Neste estudo, nós examinamos os padrões de coexistência do fitoplâncton e os possíveis determinantes da variação espacial na composição das comunidades em 9 lagoas do sul do Brasil. Nós testamos se as espécies fitoplanctônicas apresentam um padrão não aleatório de coexistência através de um modelo nulo e se distância espacial ou a distância ambiental possuem relação com a similaridade na composição das comunidades. Nós também investigamos se as variáveis ambientais locais são bons preditores da abundância das espécies. Na análise de coexistência, nós não observamos um padrão significativo de estruturação dentro das lagoas, mas encontramos um padrão não-aleatório e segregado das espécies ao longo das lagoas, indicando que as espécies coexistem menos do que o esperado ao acaso nesta escala. A similaridade na composição das comunidades mostrou correlação significativa com a distância ambiental, mas não foi correlacionada com a distância geográfica entre os sítios. Além disso, a variação na abundância das espécies apresentou correlação significativa com as variáveis ambientais locais (transparência da água, condutividade, nitrato e ortofosfato). Em conclusão, nossos resultados apoiam a visão de que a variação espacial do fitoplâncton é melhor explicada pelo ambiente local. Nós não testamos a influência de variáveis históricas e filogenéticas sobre esses padrões, que podem ser temas de estudos futuros, a fim de elucidar ainda mais essas questões. / For decades, the main hypothesis in microbial ecology was that local environment would be the only mechanism structuring communities and patterns of species coexistence. However, recent studies have provided evidence that processes related to dispersion can be as important as the local environment for shaping biological communities, stimulating new debates on the classical hypothesis about the ubiquity of microorganisms. In this study, we examined the phytoplankton coexistence patterns and possible determinants of spatial variation in community composition in 9 lakes of southern Brazil. First, we use a null model analysis for tested whether the phytoplankton species show a nonrandom pattern of coexistence. We also tested whether spatial distance or environmental distance act as potential factors controlling community composition. Finally, we investigated whether local environmental variables are good predictors of species abundance. We did not observe a significant pattern of coexistence species in the within-lakes analysis, but we found a nonrandom pattern of coexistence and segregation of species across-lakes analysis, indicating that the species coexist less than expected by chance on this scale. Phytoplankton community composition showed a strong positive correlation with the environmental distance but was not correlated with the geographical distance between sites. Moreover, the variation in the abundance of species showed significant correlation with local environmental variables (water transparency, conductivity, nitrate and orthophosphate). In conclusion, our results support the view that the spatial variation of phytoplankton is best explained by the local environmental conditions. We suggest that the influences of historical and phylogenetic variables on these patterns, here not measured, be analyzed in future studies, to further elucidate these questions.

No description available.

Orientador: Reinaldo José da Silva / Abstract: Species differ in their environmental preferences, leading to processes in the community based in gradient variations that decrease as the distance increases. This process diminishes faster in heterogeneous landscapes, especially in solid areas of bands like South American open diagonally, that is characterized by horizontal stratification environments, presenting a landscape mosaic that extends from the Caatinga to the regions of Chaco, through the Pantanal. The Cerrado, Pantanal and Caatinga are characterized by great diversity of vertebrate anuran species, including the host Leptodactylus fuscus. These regions are inserted in the open diagonal South American. The composition and the relationship between parasite-host infra-populations of helminth species associated with L. fuscus specimens were described in three different regions of Brazil. Amphibians are found in a variety of habitats and exhibit different reproductive and behavioral patterns, considered good models to study parasitology. The study evaluated if there is a decay on the similarity between geographically distant communities and concluded that these two variables are positively associated. This study assessed 155 hosts and found a total of 16 taxa: Aplectana membranosa, Aplectana pintoi, Schankiana formosula, Cosmocerca podicipinus, Oswaldocruzia lopesi, Oxyascaris necopinus, Glypthelmins linguatula, Catadiscus cf. marinholutzi, Physaloptera sp., Physalopteroides sp., Rhabdias sp., metacercariae (Diplostomid... (Complete abstract click electronic access below) / Resumo: As espécies diferem em suas preferências ambientais, isto conduz a processos na comunidade baseados em variações de gradiente que decrescem com o aumento da distância, sobretudo em faixas de áreas contínuas como a diagonal aberta sul-americana, apresentando um mosaico paisagístico que se estende desde a Caatinga até as regiões do Chaco. O Cerrado, Pantanal e Caatinga são caracterizados pela grande diversidade de espécies de vertebrados anuros, incluindo o hospedeiro Leptodactylus fuscus, e estas regiões estão inseridas na diagonal aberta Sul Americana. Foi descrita a composição e a relação entre parasita-hospedeiro de infra-populações das espécies de helmintos associados com espécimes do Leptodactylus fuscus em três regiões diferentes do Brasil. Os anfíbios são encontrados em uma variedade de habitats e exibem diversos padrões reprodutivos e comportamentais, por isso são considerados bons modelos de estudo para a parasitologia. O trabalho avaliou se ha um decaimento na similaridade entre comunidades geograficamente distantes, e concluiu que estas duas variáveis se associam positivamente. Foram avaliados 155 espécimes de hospedeiros totalizando 16 taxas de helmintos, Aplectana membranosa, Aplectana pintoi, Schankiana formosula, Cosmocerca podicipinus, Oswaldocruzia lopesi, Oxyascaris necopinus, Glypthelmins linguatula, Catadiscus cf. marinholutzi, Physaloptera sp., Physalopteroides sp., Rhabdias sp., metacercárias da Família Diplostomidae, nematodas da Família Cosmocercidae, ... (Resumo completo, clicar acesso eletrônico abaixo) / Mestre

Retail automotive literature that examines how the distance between a retail automotive facility and the prospective purchaser affects market performance is limited. Primary data for this study indicates that distance and purchase in the retail automotive sector move in opposite directions. This study examines similar goods that have high barriers of entry and proposes other methods of increasing market reach. This is a study of the conditions that affect the market performance for imported luxury vehicles. First, is the effect of distance on purchase decisions. Vehicular sales drop the further away a customer is from a car dealership. We call this phenomenon distance decay. Distance decay is defined as: the interaction between two locations declines as the distance between then increases. Secondly, when similar brands are viewed as substitutes, the consumer will choose the brand with the closest automotive service department to their residence or place of employment. Thirdly, door-to-door selling can decrease distance decay. Lastly, pick-up and delivery service can decrease distance decay. Data from 30,936 prospects and individuals who entered, phoned, or emailed a dealership inquiring about purchasing a new Audi were used in the study. These prospects will be categorized by who intended to buy and who actually purchased a car. In addition to the prospects, data from 6,153 individuals who purchased a new Audi from four Audi dealerships in the greater Philadelphia area and from the framed field experiment were used in the study. These categories will then be further labeled by ZIP code and city to determine the effects of distance. Then, possible solutions will be performed on test groups to determine what alternatives from other industries can be used to improve market performance involving long distances. / Business Administration/Interdisciplinary

The aim of this study was to gain a deeper understanding over tourism contribute to a vibrant city. This will be explored based on a campsite located near Örnsköldsvik city center and how it affects the city retail. This campsite is under investigation by the municipality to be relocated, to make room for exclusive and attractive residences. This suggestion has been criticized by the residents in the municipality due to their thoughts that it will conduce to negative effects on the city retail. The study is based on a qualitative research method. Five interviews have been made to investigate the tourists meaning to retail and if distance has an effect. This study showed that the tourists at the city campsite are important for retail in the city center. It enables an increased movement of people in the city, which leads to a vibrant city center. It also contributes to promoting social sustainability, by enabling relatively cheap accommodation close to the city center. This in turn generates inclusion and access to service for a greater variety of social groups. However, it did not account for a large amount of consumption. The geographical distance proved to be important for the propensity to visit the restaurants, while accessibility was more important than the physical distance for the number of tourists who visited the shops.

Fruits and flowers house microbial communities that are unique from the rest of the plant. While a great deal is known about a handful of symbiotic microbes associated with roots and leaves, the microbial communities of fruits and flowers have received considerably less attention. Fruits are reproductive tissues that house, protect, and facilitate the dispersal of seeds, and thus they are directly tied to plant reproductive success. Fruit and flower microbial communities may, therefore, also impact plant fitness. This dissertation examines how fruit bacterial communities, as determined using the 16S rRNA gene marker, are shaped across spatial and environmental gradients and the role of pollinators in shaping floral bacterial communities among natural populations of Solanum carolinense. There have been limited studies on how spatial distance influences bacterial communities found in and on fruit tissue and the role of pollinators in shaping floral bacterial communities. The first study addresses how bacterial communities in fruit change across similar environmental conditions at fine spatial scales (2 to 450 m). Overall, no differences were found in observed richness or bacterial community composition. Next, the role that generalist pollinators might play in shaping these communities was tested using pollinator exclusion cages. Here we found that generalist pollinators do not play a large role in shaping floral bacterial communities in Solanum carolinense. Comparing bacterial community diversity between caged and uncaged flowers, via PCoA we found no significant clustering of samples. In contrast, significant clustering was detected between flowers and bee pollen baskets. Together these results suggested that environmental factors may be more important in shaping floral bacterial communities. To test this, we sampled 9 populations along a 337 km latitudinal transect and again used the 16S rRNA gene to characterize bacterial communities. We did not identify a significant correlation between distance and bacterial community composition in either the total nor endophytic community in the fruit. Results from these studies suggest that while there is some evidence for environmental effects shaping fruit and flower communities, other factors such as host selection (e.g., secondary compounds in fruit) also likely play an important role in shaping bacterial communities. / Doctor of Philosophy / Fruit and flowers are directly tied to plant reproduction, yet little is known about the bacterial communities associated with these important organs, especially compared to other plant tissues. This dissertation aims to address gaps in our knowledge regarding how spatial distance and pollinators influence fruit and floral bacterial communities. Specifically, how does bacterial community composition (what bacteria taxa are present or not and how abundant the bacterial taxa are) change based on spatial distance. Studies were conducted at both fine (under 0.5 km) and broad (337 km) scales to determine if a pattern was observed between increasing distance and how distinct the bacterial community composition is. There was no significant correlation between spatial distance and bacterial community composition at fine spatial distances, however there were high levels of dissimilarity in the bacterial communities sampled across fine spatial scales. This led to the investigation of pollinators, which directly interact with flowers and may act as a source of bacterial community transmission in the flower and fruit bacterial communities as they move around the landscape. To address the role that pollinators play in shaping bacterial communities in the fruits an exclusion cage study was used to prevent pollinators from interacting with a subset of flowers. Pollen baskets (pollen that was collected by the pollinator and is found on the legs of pollinator) were also collected from pollinators to determine what bacteria may be present on the pollinator. Pollen baskets may also represent what bacteria may have been picked up by the pollinator at the site. It was observed that caged and uncaged samples were similar to one another, which suggest that pollinators were not adding new bacteria nor changing the bacterial composition in the flowers. Yet, the pollen baskets (from the pollinator) were unique from the caged and uncaged floral samples. To further investigate what factors may be at play in shaping the fruit bacterial community the transect was expanded to 337 km to determine if there was an observable relationship between spatial distance and bacterial community composition. The relationship between spatial distance and bacterial community composition was not significant in either the endophytic (internal bacterial community) or the total fruit bacterial community (which represented external and internal bacterial communities). Similar to the fine spatial scale study, there were high levels of dissimilarity in the bacterial community that was observed across sites. Results from these studies may suggest that there are other factors that play a larger role in shaping bacterial communities in fruit and flower tissue. These could include the host plant and the production of secondary compounds, which in some cases can act as antimicrobial compounds, and enhance or inhibit the growth of specific bacterial taxa. Environmental factors such as wind and rainfall may have influenced the bacterial community composition. It is likely that environmental factors play a role in shaping floral and fruit bacterial communities. However, it is still unclear what factors shape fruit and floral bacterial communities are. This study provides the foundation for future studies to address additional factors that shape fruit and flower bacterial communities.

No description available.

Health studies often rely on aggregated instead of individual-level data to protect patient privacy. However, aggregated data are subject to the modifiable areal unit problem (MAUP), meaning results of statistical analyses may differ depending on the data's scale and areal unit. Past studies have suggested MAUP is context-specific and analyzing multiple spatial scales may provide richer understandings of examined phenomena. More research is needed to understand the role of scale and areal unit in health-related analyses. This study examines associations between surface mining and birth outcomes from 1989 to 2015 in Central Appalachia at the individual; postal; county; and county-sized, non-administrative scales. Evidence from previous studies suggests associations exist between health outcomes and county-level measures of mining activity. This is the first study to examine associations between mining and birth outcomes at more spatially refined exposure estimates. We identified surface mines using Landsat imagery and geocoded birth records. Airsheds, used to quantify the influence area of potential airborne pollutants from surface mining activity, were built using HYSPLIT4. The frequency values of each airshed that intersected each geocoded birth record were summed. These cumulative frequency airshed values were then aggregated. Finally, we implemented multiple regression models, each at a different scale, to examine associations between airsheds and birth outcomes. Results suggest MAUP has minimal impacts on the statistical results of examining associations between surface mining and birth outcomes in Central Appalachia. Results also indicate surface mining is significantly associated with preterm birth and reduced birthweight at each scale. / Master of Science / Health studies often rely on data that has been grouped together within political boundaries (e.g. counties) instead of individual-level data to protect patient privacy. However, results from analyses using grouped data may differ depending on the data's scale and areal unit, which describes the modifiable areal unit problem (MAUP). Past studies have suggested MAUP is specific to the situation being analyzed and examining multiple scales may provide richer understandings of the situation. More research is needed to understand the role of scale and areal unit choice in health-related analyses. This study examines associations between surface mining and birth outcomes from 1989 to 2015 in Central Appalachia at the individual; postal; county; and county-sized, non-administrative scales. Evidence from previous studies suggests associations exist between health outcomes and county-level measures of mining activity. This is the first study to examine associations between mining and birth outcomes at finer scales. Surface mines were identified using satellite images, and we identified the locations of birth records using the mother's home address. Airsheds, used to determine the influence area of airborne pollutants from surface mining activity, were created. We then used statistical models, to examine associations between airsheds and birth outcomes at four spatial scales. Results suggest MAUP has minimal impacts on the statistical results of examining associations between surface mining and birth outcomes in Central Appalachia. Results also indicate surface mining is significantly associated with preterm birth and decreased birthweight in grams at each scale.