This thesis is based on two readings on death. The first one is Martin Heidegger&rsquo / s Being and Time chapter two, part one and the second one is Jacques Derrida&rsquo / s Aporias. The first reading is based on the phenomenological analysis of death. The line of argument of Heidegger is figured out. The second reading is based on Derrida&rsquo / s deconstruction of Heidegger&rsquo / s account of death in Being and Time. The thesis and the conclusion part is based on the idea that, on death, these philosophers are fundamentally similar and radically different. This is shown by the comparison of these philosophers.

In this thesis the quiddity of knowledge in Kant&#039 / s critical philosophy has been investigated within the historical context of the problem. In order to illustrate the origins of the subject-matter of the dissertation, the historical background of Kant&#039 / s views on the theory of knowledge has been researched too. As a result of this research, it is concluded that Kant did not invent a new philosophical problem, but he tried to improve a decisive solution for one of the oldest question of history of philosophy i.e., &ldquo / How is synthetic a priori knowledge is possible?&rdquo / The theoretical dimension of Kant&#039 / s theory of knowledge is reserved for this purpose. The above mentioned question is not new neither for us nor for Kant, but his answer and his philosophical stand have clearly revolutionary meaning both for us and for him. This thesis claims that his stand-point not only leads to an original epoch for the theory of knowledge, but creates a serious possibility for a new ontology explicating the quiddity of knowledge.

The aim of this thesis is to investigate the problems that use of idealizations in science leads to. Idealizations are simplifications and therefore false descriptions of how actual objects behave. Presence of idealizations in scientific theories is the reason for the problems in our understanding of confirmation of theories and also of scientific explanations. Nevertheless, idealizations are ubiquitous especially in natural sciences. Scientists have to employ idealizations because of the complexity of the real world and our limited capacity of computation. The roots of the methodology of modern science are in Cartesian philosophy. I propose that Descartes also employed idealizations in his theory of motion in the universe. Idealized worlds can be regarded as simplifications of the real world. Scientific theories are literally false but they are true in the possible worlds which are similar to the real world. Models provide the connections between idealized laws and the real world. Construction of models of the actual world is based upon idealizations which are indispensable in the theoretical sciences. Theories can be indirectly confirmed by models denoting different aspects of the phenomena.

In this thesis, Seneca&rsquo / s notion of self as self-love and the problem of self-deception are analyzed. In examining three types of self-love, &ndash / ignorant, progressing selves,&ndash / three models of self-deception are discussed. Self-deception is related to the problem of self-knowledge. I discuss the nature of self-love as self-esteem and self-preservation and self-shaping all of which are innate qualities and develop into more complex forms of knowing. Passions are concrete examples of the representations of deceived self / central to the overestimation of indifferents, the deceived self displays a pattern of reasoning that creates a paradox between what the self intends to do and what it actually appears or what the self wants to see himself as and what it actually is. In discussing various types of self-deception, it is argued that problem of deception can hardly be overcome practically even by education, although it is naturally possible. While the ignorant deceive themselves beyond their recognition, in the case of the educated selves, the tension between the knowledge of ignorance and the desire to be the person play an important role in self-deception. No one except the sage is free from self-deception. The thesis deals with the issue of self-knowing as a scarce possibility.

The main concern of the thesis is the problem of reconciliation of freedom and natural causality and to investigate how Kant makes a room for freedom. Kant, firstly, in &ldquo / Analytic&rdquo / , constitutes the conditions of knowledge upon which the objective validity of the law of causality entirely rests. This process of constitution also determines the limits of experience. On the other hand, Kant, in &ldquo / Dialectic&rdquo / , postulates freedom as a noumenal cause together with the law of causality. Transcendental freedom, in this case, is a problematic concept which transcends the limits of experience, as it seems to destruct the unity of experience. However, Kant gives up neither the law of causality nor the idea of freedom, but rather he insists upon the idea that they can exist together without contradiction by asserting the distinction between phenomena and noumena as different grounds on which these two different types of causalities rest. According to Kant both are indispensable, as the former is necessary for the knowledge and the latter is absolutely needed for morality. In this context this thesis aims to explain the objective validity of natural causality which is proved in Second Analogy and the transcendental ground of the idea of freedom which is established in the solution of Third Antinomy in Kant&rsquo / s Critique of Pure Reason. And it is discussed whether Kant&rsquo / s solution of this issue is satisfactory and legitimate or not.

The main concern of this thesis is to show Plato&rsquo / s solution of the problem of falsehood in his dialogue of the Sophist. In the Sophist, it is argued that false statements are the expressions of something which are not real by Plato. On the other hand, what is not real has been considered as what does not exist, namely, as non-being in the ontological tradition until Plato. Furthermore, non-being can neither be thought nor be stated / since thought must be thought of something which exists. Therefore, to speak of the possibility of false statements is a contradiction because it means to attempt to state nothingness. However, Plato overcomes this difficulty by asserting a different definition of non-being. Plato asserts that non-being is not necessarily opposite of being. According to this, non-being is only &lsquo / different&rsquo / from being and exists as much as being and this is possible by the combination of &lsquo / Kind of Being&rsquo / and &lsquo / Kind of &lsquo / Difference&rsquo / . In this context, this thesis purposes to show firstly how Plato renders an ontological possibility to non-being and secondly how he applies this inference to statements in order to solve the problem in the Sophist. In addition, the results of Plato&rsquo / s assumption that there is a correspondence between language and reality are discussed with respect to the problem of falsehood.

The purpose of this study is to present the metaphysical status of &ldquo / language game&rdquo / in later Wittgensteinian philosophy of language and to deal with the revolutionary role of &ldquo / language-game&rdquo / by means of Hintikka&rsquo / s interpretation of later Wittgenstein. It is usual to divide Wittgenstein&rsquo / s work into the early and the later period. The early period is based upon the picture theory of meaning, according to which a sentence represents a state of affairs. On the other hand, the later period gives special emphasis on the actions of people and the role their linguistic activities. The early period ignored factual or cognitive meaning since it relied on mirroring the structure of state of affairs by sentences. So, early period of Wittgenstein was concluded that &ldquo / whereof we can&rsquo / t speak, thereof we must be silent.&rdquo / This idea gives clues about metaphysics of early Wittgenstein. In this sense, language is treated in abstraction from activities of human beings. In the later work, Wittgenstein emphasizes everyday usage of language in &ldquo / language-game&rdquo / as social activities of ordering, advising, measuring, and counting and so on. These different &ldquo / language-games&rdquo / make up &ldquo / form of life&rdquo / . &ldquo / Language game&rdquo / with other vital notions of later Wittgenstein, as &ldquo / form of life&rdquo / , &ldquo / agreement&rdquo / establishes language matrix. So, later period of Wittgenstein is a rejection of his early period. Actually, his treatment of philosophy and philosopher is different from his early period. In addition to this, later Wittgenstein mainly focuses on the principle of &ldquo / meaning=use&rdquo / which is called contextual theory of meaning. In his later period, Wittgenstein aims to bring back words from metaphysics to everyday usage. On the other hand, metaphysics still plays a role in his later period as his early period, although he altered his early philosophy of language. To sum up, the notion of &ldquo / language-game&rdquo / is conceptually/ ontologically prior to its rules. In this sense, Wittgenstein forms &ldquo / language-game&rdquo / as a model for the other social activities of human beings. Furthermore, &ldquo / language-game&rdquo / is regarded as a bridge between language and reality and it shows &ldquo / language-game&rdquo / s revolutionary role in later Wittgenstein.

The aim of this thesis is to investigate the passions in Cartesian philosophy. It analyses the nature, characteristics and the causes of passions as discussed by Descartes in his correspondence with Princess Elizabeth and his last book The Passions of the Soul (Les passions de l&rsquo / &acirc / me). This thesis purports to explain Descartes&rsquo / ethical view by examining the physical mechanism of the passions and their relation to the soul. The reason, will and their essential roles in Cartesian ethics are discussed.

Submitted by Diogo Misoguti (diogo.misoguti@gmail.com) on 2018-04-18T13:18:24Z No. of bitstreams: 1 Tese-15860.pdf: 879437 bytes, checksum: c869f5350ca0a0b0f13be8ba348f8fa5 (MD5) / Approved for entry into archive by Mariusa Loução (mariusa.loucao@unifesp.br) on 2018-04-18T13:20:15Z (GMT) No. of bitstreams: 1 Tese-15860.pdf: 879437 bytes, checksum: c869f5350ca0a0b0f13be8ba348f8fa5 (MD5) / Made available in DSpace on 2018-04-18T13:20:15Z (GMT). No. of bitstreams: 1 Tese-15860.pdf: 879437 bytes, checksum: c869f5350ca0a0b0f13be8ba348f8fa5 (MD5) Previous issue date: 2016 / O uso da biologia molecular como ferramenta de diagnóstico microbiológico vem se expandindo no setor da medicina laboratorial. Novas plataformas aparecem com a prerrogativa de facilitar e acelerar o processo de análise. O uso de sistemas automatizados, que realizam extração, amplificação e detecção de ácidos nucleicos dentro da mesma plataforma, permite maior precisão e facilidade ao desenvolvimento de um novo teste por apresentarem todos os processos acoplados e reagentes disponíveis para o processo. Dentre as plataformas automatizadas, uma das que se destacam é o BD Max™ (Becton Dickinson Diagnostics). O sistema BD Max é uma plataforma automatizada aberta, que combina a extração de ácidos nucleicos, PCR (Reação de Polimerização em Cadeia) em tempo real e detecção dentro do mesmo instrumento, oferecendo a opção de usar os testes aprovados pelo Food and Drug Administration (FDA) e também, testes desenvolvidos pelo usuário. Com o objetivo de testar os recursos que a modalidade aberta da plataforma BD Max oferece, foram desenvolvidos três estudos distintos para o diagnóstico molecular de agentes infecciosos direto de amostras clínicas. Estudo 1: O objetivo deste estudo foi validar um teste multiplex usando a tecnologia PCR em tempo real no sistema aberto BD MAX™, para detectar o complexo Mycobacterium tuberculosis (CMT), complexo Mycobacterium avium (CMA) e Mycobacterium spp. (PAN) diretamente de amostras clínicas. Quando os resultados do novo teste foram comparados com os resultados da cultura, a reação de PCR apresentou especificidade de 97,1%, 100% e 100% para CMT, CMA e PAN, respectivamente. Estudo 2: O objetivo deste estudo foi validar um teste multiplex usando a tecnologia PCR em tempo real no sistema aberto BD MAX™ para detectar o grupo Mycobacterium abscessus (GMA), complexo Mycobacterium fortuitum (CMF) e Mycobacterium chelonae (MC), diretamente de amostras clínicas. Quando os resultados do novo teste foram comparados com os resultados da cultura, uma concordância de 97%, 100% e 99% para GMA, CMF e MC, respectivamente foi observada. Estudo 3: O objetivo deste estudo foi validar um teste multiplex usando a tecnologia PCR em tempo real no sistema aberto BD MAX™ para detectar e identificar Achromobacter xylosoxidans (AX), Burkholderia cepacia (BC), Pseudomonas aeruginosa (PSA) e Stenotrophomonas maltophilia (SM) diretamente de amostras respiratórias de pacientes portadores de Fibrose Cística (FC). Quando os resultados do novo teste foram comparados com os resultados da cultura, uma alta concordância foi observada entre as duas metodologias. Conclusão: Os 3 testes desenvolvidos provaram ser específicos e sensíveis para detectar por PCR em tempo real microrganismos causadores de infeção direto da amostra clínica. A plataforma automatizada BD Max provou ser uma excelente ferramenta para a realização de testes moleculares automatizados. / The use of molecular biology as a tool for microbiology diagnostic has been expanding in the laboratory routine. Despite of the strong growth of the area, companies can not afford the demand about epidemiological changes around the world and for this reason laboratories opt to develop their own methods. New platforms appear with the prerogative to facilitate and accelerate the analysis process. The use of automated sample-in results-out platforms allows higher precision and facilitates the development of a new test by presenting all attached processes and reagents available for the test. Among platforms that best fits this profile, the BD Max™ (BD Diagnostics) is one of the most used ones. The BD Max system is an automated open platform that combines extraction and real time PCR in the same instrument, offering the option of using tests approved by the FDA or the open platform mode for userdeveloped test. In order to explore the BD Max open mode platform, three differents studies were developed to detect the microorganisms that causes infection directly from clinical samples. Study 1: A multiplex real time PCR was validated on the BD MAX™ open mode system to detect Mycobacterium tuberculosis complex (MTC), Mycobacterium avium complex (MAC) and Mycobacterium spp. (PAN) directly from clinical specimens. When compared to culture results, the new BD MAX PCR test presented specificities of 97.1%, 100% and 100% for MTC, MAC and PAN, respectively. Study 2: A multiplex real time PCR was validated on the BD MAX™ open mode system to detect Mycobacterium abscesses Group (MAG), Mycobacterium fortuitum complex (MFC) and Mycobacterium chelonae (MC) directly from clinical specimens. When compared to culture results, the new BD Max PCR test presented an overall agreement of 97%, 99% and 100% for the detection of MAG, MFC and MC, respectively. Study 3: A multiplex real time PCR was validated on the BD MAX™ open mode system to detect Achromobacter xylosoxidans (AX), Burkholderia cepacia (BC), Pseudomonas aeruginosa (PSA) and Stenotrophomonas maltophilia (SM) directly from clinical specimens collected from Cystic Fibrosis patients. When culture results were compared to the new BD Max PCR test results, a high overall agreement were observed between both methodologies. Conclusion: All 3 tests proved to be specific and sensitive to detect different microorganisms associated with infections, directly from clinical samples. The BD Max proved to be an excellent tool for automated molecular tests.

En la presente investigación se adaptó y se determinaron las propiedades psicométricas (validez, fiabilidad) y la utilidad diagnóstica del cuestionario Quality of life Bipolar Disorder [QoL. BD] en pacientes bipolares chilenos atendidos en centros de salud públicos y privados de Santiago. Se entrevistaron a 63 sujetos (grupo clínico n=32 y grupo no clínico n=31) a los cuales se le administró el QoL. BD, SF-36, MINI, YMRS, HAM-D y el respectivo consentimiento informado. Se obtuvo una elevada fiabilidad (α =0.95) y alta validez referida a criterio externo (r= entre 0.453 y 0,819; p<0,001). En términos de utilidad diagnóstica, se estimó un punto de corte de 170 puntos (sensibilidad de 87,9% y especificidad de 80%). Se concluye que el QoL. BD muestra propiedades psicométricas adecuadas, hallándose evidencia de confiabilidad y validez, además de una adecuada sensibilidad y especificidad para distinguir entre una percepción negativa y positiva de calidad de vida en pacientes bipolares chilenos