Intensively managed forests of the Southern United States are among the most important sources of wood fiber and timber in this country. There is a great deal of concern that disturbances associated with trafficking by heavy machinery might diminish long-term soil-site productivity. However, determining the effect of harvesting disturbance and silvicultural treatments on the long-term productivity of pine plantations is difficult because, in addition to harvesting effects, growth distributions are affected by changes in climate, silviculture, and genetics. The primary objectives of this study were to determine (1) whether logging disturbances under operationally realistic circumstances affect soil quality, hydrologic function, and loblolly pine (Pinus taeda L.) productivity on wet pine flats, and (2) whether intensive forest management practices mitigate disturbance effects if they exist. Three 20-ha loblolly pine plantations located on wet pine flats in South Carolina were subjected to combinations of wet- and dry-weather harvesting and mechanical site preparations. Changes in soil-site productivity after five years were evaluated using a new rank method. The key advantage of the rank change method is that it is largely independent of the confounding factors (e.g. genetics, silvicultural practices, and climate) that affect comparisons of tree growth and soil quality between growth cycles. After five years, loblolly pine site indexes (base age 25) ranged between 13 and 33 m, and production ranged between 0.5 and 95 Mg ha-1. Soil bulk densities increased from 1.15 to as high as 1.44 g cm-3 after harvesting; by age 7 years it had decreased to approximately 1.25 g cm-3. At the sub-stand scale (0.008 ha), visually assessed soil physical and harvesting residue disturbances had little influence on relative changes in soil-site productivity after five years. Factors that specifically reflect site drainage were the most influential on changes in productivity. At the operational scale (3.3 ha), there were no differences between wet- and dry- harvested sites as long as bedding was applied as a site treatment. These results indicate that when standard site preparation is employed, fertile, wet pine flats such as these are resilient in that they recover from severe, but operationally realistic, harvesting disturbances. / Ph. D.

In the upland hardwood forests of the southern Appalachians, management tools are needed based on the characteristics of the site to quantify the site quality where no accurate maps of site quality exist. Three studies were conducted to achieve this objective. The first study tested if independent measures of forest productivity, based on vegetation and environment, in a six-county study area in the Blue Ridge Mountains in North Carolina would correlate with measures of forest productivity obtained from U.S. Forest Service Forest Inventory Analysis (FIA) data. Specific hypotheses included: FIA measures of forest productivity are related to one another; FIA measures of forest productivity are related to FIA-measured landscape parameters; and FIA measures of forest productivity are related to independent measures of forest productivity based on landscape parameters and soil characteristics. Four predictive indices of forest productivity were used; three were generated in a geographic information system (GIS). FIA measures of forest productivity were not significantly correlated to FIA measured landscape parameters. FIA site productivity classes were significantly correlated to FIA measures of site index. Independent measures of forest productivity, particularly the Moisture Regime Index (MRI) and the Forest Site Quality Index (FSQI), were significantly correlated to FIA measures of site index. Topography can be used to delineate site quality, but the addition of soil depth can prove to be useful in the estimation. The second study was designed to develop methods, based on field and digital data, to identify colluvial soils in the central Ridge and Valley of southwestern Virginia. Two hypotheses were tested. First, on the linear side slopes of the study area, where site quality is low in stands with subxeric to xeric moisture regimes, vegetation and topography can indicate colluvial soils. A second hypothesis tested if the topographic signature of colluvial soils could be identified geospatially with a digital elevation model. Results indicated that the MRI and the Terrain Shape Index predicted the presence of colluvial deposits in the study area. The basal area of yellow-poplar was positively associated with colluvial soils. A GIS-based model found the slope difference of colluvial soils to be less steep than residual soils as the size of the neighborhood increased. The final study determined if measures of site quality in the Blue Ridge Mountains of North Carolina were related to the water budget. Specifically,the hypothesis that site index could be predicted by variables that represented the inputs, usage, and supply of water was tested. A second hypothesis questioned if site quality classes could be predicted by a combination of topography and the annual water budget. Regression models predicted site index to be a function of topography, available water supply, and the annual water budget, but the accuracy was low (R2=0.11 and 0.13). A classification approach yielded better results. Incorporating the annual water budget into the FSQI increased classification accuracy of predicted site index by 50%, and decreased the number of sites misclassified by one class by 8%. Where accurate maps of site quality do not exist, the MRI, the abundance of yellow-poplar, and the modified FSQI may be used to delineate site quality for site-specific management and, ultimately, greater return on investment for the landowner. / Ph. D.

Local site conditions can greatly increase the intensity and character of earthquake shaking and, thus, the extent and type of structural damage. The removal and replacement of in-situ soils with compacted gravel-sized volcanic rock has been prevalent in the Icelandic foundation subgrade construction practice for decades, despite the unknown seismic site response characteristics of the fill (e.g., the predominant frequency and relative site amplification). To fill this knowledge gap, over 500 hours of microtremor measurements were made at six study sites located throughout the Reykjavík, Iceland, capital region. Measurements recorded at various construction stages (e.g., the in-situ or pre-excavation, post-excavation, intermediate grades, and final grade) reveal the change in site response characteristics before and after gravel fill placement. The data was analyzed using the horizontal-to-vertical spectral ratio (HVSR) technique over a bandwidth of 0.3 to 25 Hz. Generally, the pre-excavation condition had a predominant site frequency between 3.5 and 7 Hz with relative amplification between 3.8 and 3.9 times. The placement of gravel fill atop dense to very dense silty sand underlain by bedrock shifts the predominant frequency between 10 and 16 Hz with a relative peak amplification between 2.5 and 5.3 times, generally increasing with fill thickness. Fill underlain by undulating lava rock also results in a higher a predominant frequency between 9 and 10.5 Hz, but little change in relative site amplification occurred at these frequencies (between 0.95 and 1.2 times). This dissimilarity is due to the unique lava rock HVSR signatures which have large amplification values (between 2.6 to 3.9 times) throughout the high-band frequency range. Additional investigations of sites underlain by lava rock are required to draw stronger empirical trends. The data set produced by this study can serve as a useful tool for the local geotechnical and seismological communities to mitigate seismic risk for the capital region. / Master of Science / Problematic soil conditions can greatly increase the intensity and character of earthquake shaking and, thus, the extent and type of building damage. The removal of native soils and replacement with compacted gravel-sized fill has been the predominant building foundation subgrade construction method in Iceland for decades. The practice of removal and replacement is one of the oldest and conceptually simplest approaches of site improvement to reduce settlement and increase soil strength. However, the understanding of how compacted gravel fill responds to earthquake shaking was nonexistent in literature. To fill this knowledge gap, the response characteristics of compacted gravel fill were derived using the horizontal-to-vertical spectral ratio (HVSR) and standard spectral ratio (SSR) analysis techniques from a data set of over 500 hours of experimental in-field measurements. Measurements were recorded at various construction stages (e.g., pre-excavation or native soil, post-excavation, intermediate fill grades, and the final fill grade) to reveal the change in site response characteristics before and after gravel fill placement. The findings presented in this thesis can serve as useful information for the local geotechnical and seismological communities to mitigate seismic risk (e.g., the probability of building damage and/or loss of lives) of structures with compacted gravel fill subgrades in the Reykjavík, Iceland capital region.

Equations for modeling the height-age pattern of forest trees or stands are typically developed for a given species in a specified region. In order to adequately model height-age patterns, the resulting equations are often quite complex. This study addresses the question of how to increase the accuracy and precision of the prediction of height-age relationships through the use of localized equations. Although the pattern of height-age relationships of loblolly pine plantations was studied, the methodology should prove valuable for other species as well. The Schumacher logarithm of height-reciprocal of age model was fitted to data from loblolly pine plantations to attain an average guide curve. Various methods of localizing this equation to a particular stand were examined. The methods are based on empirical Bayesian, maximum likelihood, and Kalman filter theory. All of these methods employ the general concept of feedback in localizing the simple equation. The best of these various models is compared with the unadjusted model and a more complex polymorphic equation. The adjusted model compares favorably with these other two models. / Master of Science

Site preparation is fundamental for establishing loblolly pine (Pinus taeda L.) plantations, but long-term sustainability of plantations established using mechanical treatments is in question because of concerns regarding soil tillage and the removal of harvest residue and soil organic matter. A study was installed in 1981 on 12 locations in northeastern Georgia and west-central South Carolina to evaluate pine plantation response to mechanical site preparation. Site preparation treatments induced gradients of organic matter manipulation and soil tillage. The treatments included: Control, Chop/Burn, Shear/Disc, Shear/V-Blade, Shear/Rake, and Shear/Rake/Pile. Research was conducted to address the following objectives: (i) compare rotation-age forest response to several intensive site preparation treatments used to establish pine plantations in the Piedmont of the southeastern United States; (ii) correlate growth response with the gradients of soil organic matter removal, soil tillage, and hardwood control; (iii) determine the influence of intensive management on the amount of carbon contained in pine plantations. All site preparation treatments increased year-18 volume accumulation compared to the control treatment. Chop/Burn and Shear/Disc treatments, with pine volumes of 214 m3 ha-1 and 232 m3 ha-1, respectively, conserved harvest residue and out-performed the Shear/Rake treatment (191 m3 ha-1), which completely removed harvest residue. Treatments that included tillage provided growth benefits that lasted throughout the rotation even when tillage was accompanied by complete organic matter removal. Hardwood competition had the greatest influence on pine volume accumulation, explaining over 54% of the variation in pine growth at age 18. Treatments that included tillage most effectively controlled hardwood competition. At year 18, site preparation treatments significantly affected soil organic matter (SOM) content; however, soil nitrogen, foliar nitrogen, bulk density, and macroporosity were not affected by site preparation. All treatments were equally deficient in foliar nitrogen. The Shear/Disc and Shear/Rake/Disc treatments had a significantly positive relationship between foliar nitrogen and pine volume. These treatments had lower hardwood basal areas (below 15%), indicating that once hardwoods were controlled, nitrogen became limiting to pine growth. Using pre-harvest characterization data, carbon accumulation during old-field succession increased fourfold compared to agricultural sites on the nearby Calhoun Experimental Forest. Carbon accumulation on these old-field loblolly pine sites reached quasi-equilibrium after 40 years as shown by uncut reference stands. Site preparation significantly affected the amount of soil C in the upper 20 cm of the soil. Those site preparation treatments that removed harvest residue and accelerated SOM decomposition through tillage had the lowest soil carbon levels. The Shear/Rake/Disc treatment had 10% lower soil carbon content than the Control and Shear/V-Blade treatments. / Master of Science

Unmanned vehicle systems are becoming more important in the future of the military and in commercial applications. These systems are used to prevent humans from entering dangerous situations or to automate dull tasks. In order to facilitate rapid development of these systems, testing procedures and infrastructure need to be created. Once developed, the performance characteristics of unmanned vehicle systems can be determined and compared to similar systems. This information will be beneficial to system developers and potential customers. In order to provide the infrastructure and test procedures to the unmanned systems community, the Joint Robotics Program created the National Unmanned Systems Experimentation Environment (NUSE2). NUSE2 consists of a variety of military organizations and academic resources, including the Joint Unmanned Systems Test Experimentation and Research (JOUSTER) site at Virginia Tech. JOUSTER was tasked specifically with creating a mobile instrumentation platform capable of providing wireless communications, data collection, and video coverage of a testing site. This thesis presents the system designed and created to meet this need. For the first time, a mobile instrumentation platform has been created to specifically support unmanned systems research. Additionally, the performance characteristics of this system have been fully evaluated and will serve as a benchmark for future improvements to the system. / Master of Science

Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal. / Chez les procaryotes, la réplication du chromosome peut entraîner la dimérisation des deux nouveaux chromosomes par recombinaison homologue. La fréquence de ce phénomène est particulièrement élevée dans la région du terminus de réplication du chromosome de E. coli, notamment aux sites Hot et TTiZ. Si le dimère chromosomique n'est pas résolu avant la partition dans les cellules-filles, il y aura filamentation cellulaire et aberration chromosomique. Pour contrer ce phénomène, E. coli a mis en place un système de résolution de dimères, soit le système de recombinaison spécifique de site Xer. Chez E. coli, ce système comprend les recombinases XerC et XerD et le site chromosomique dif situé dans la région du terminus de réplication. Les recombinases s'arriment d'abord aux sites dif des chromosomes formant le dimère, puis il y a coupure et échange des brins libérant ainsi les chromosomes à leurs formes monomères. La répartition de ce système de résolution de dimères est assez bien documentée parmi les bactéries Gram négatives. Cependant, peu d'études se sont penchées sur les bactéries Gram positives. Le but de notre étude est de détecter la présence de ce système chez treize espèces de bactéries Gram positives du groupe des bactéries lactiques, certaines étant très importantes dans l'industrie alimentaire. Plusieurs techniques ont été employées, dont l'hybridation Southern, l'amplification génique avec amorces dégénérées issues des séquences des recombinases déjà connues, l'amplification génique dite "inverse", ainsi que le séquençage. Nous avons également utilisé un programme informatique afin de dégager la phylogénie et les ressemblances entre les recombinases de Gram positives et entre celles-ci et les recombinases déjà connues des bactéries Gram négatives. Par hybridation Southern, nous avons réussi à démontrer la présence d'un site analogue au site dif de E. coli chez Lactobacillus casei. De plus, l'amplification génique avec amorces dégénérées a montré que cette dernière espèce ainsi que les espèces Lactobacillus plantarum souche 8014, Lactobacillus plantarum souche 14917 et Lactobacillus bulgaricus 737 possèdent toutes au moins une recombinase de type Xer. Nous n'avons pas obtenu de séquences pour les autres espèces de bactéries, peut-être à cause d'un manque d'homologie entre les amorces et l'ADN ciblé. Les régions amplifiées de ces quatre souches, correspondant à environ 430 pb du bout C-terminal de la recombinase, ont été séquencées, puis des amorces ont été fabriquées à partir de la séquence de L. casei. Ces amorces devaient amplifier les régions flanquant la séquence connue (amplification "inverse"), permettant ainsi d'obtenir une séquence complète du gène. Nous n'avons pas obtenu de résultats avec cette technique, peut-être à cause de la formation de boucles C-G dans l'ADN simple-brin lors de certains cycles de l'amplification. Les quatre séquences ont ensuite été comparées et une phylogénie a été établie. Nous avons trouvé que ces séquences formaient un groupe phylogénique fortement apparenté et qu'elles ressemblaient le plus aux séquences XerD des bactéries Gram négatives, ces dernières ressemblances surpassant même celles avec la recombinase XerD de la bactérie Gram positive B. subtilis. Ces derniers résultats suggèrent la possibilité d'une évolution différente des recombinases XerD à l'intérieur du groupe des Gram positives que nous avons examiné.

Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.

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Concentration ratios are used to determine the transfer of nuclides from soil to biota to fauna. Some nuclides have limited associated data though, this has not prevented predictions from being performed at sites without associated data. These ratios are site specific and are not fully applicable when applied to other locations. A recent literature review for a waste repository performance assessment determined that a significant portion of the environmental data was based on recursively published material. To address this deficiency neutron activation analysis (NAA) was used to determine concentration ratios of certain biota. Three sites, two riparian and one shrub steppe, were sampled in the eastern Washington Columbia basin, near the Hanford site. Two hundred and fifty eight samples of opportunity were collected. This included 15 soil samples, 10 water and sediment samples, 40 different species of biota, and 2 terrestrial animal species and 3 aquatic animal species. These samples were prepared for NAA by drying, weighing, and in certain cases ashing to improve detection efficiency. After activation, the samples were placed in a HPGe detector to perform spectral analysis. The concentration results of 26 elements of interest are presented, along with newly established concentration ratios for all of the species sampled. / Graduation date: 2013