This thesis answers the question of whether and to what extent the goals of PISA align with the Manitoba Government’s goals and priority action areas, and whether the published results of PISA 2012 provide information that addresses the Manitoba Government’s education goals and priority action areas, within the context of mathematics. This thesis provides a qualitative analysis of three PISA 2012 documents, and explores and compares Manitoba PISA 2012 achievement data to Manitoba grade 9 mathematics credit achievement data for English and Français program students, EAL and Aboriginal students to determine whether and to what extent the goals of PISA align with the goals and priority action areas of Manitoba Education and Training, and whether the published results of PISA 2012 provide information addressing the goals and priority action areas of Manitoba Education and Training, within the context of mathematics. / February 2017

Lyme disease is the most common tick-borne disease in North America, with approximately 35,000 cases reported to the Centers for Disease Control in 2008. The genome of its causative agent, Borrelia burgdorferi, encodes for a set of genes involved in the metabolism and regulatory activities of the second messenger nucleotide, cyclic-di-GMP (c-di-GMP). Rrp1 is a response regulatory-diguanylate cyclase, and its regulatory capability is likely mediated via production of c-di-GMP, as it lacks a DNA-binding domain. One known class of c-di-GMP effector/binding proteins are those that harbor a PIlZ domain. The genome of B. burgdorferi strain 5A4 encodes for one chromosomally-carried PilZ domain, which we have designated PlzA. Additionally, certain B. burgdorferi strains encode for a second PilZ domain-containing protein (PlzB) which is plasmid-carried. Both PlzA and PlzB were found to bind specifically to c-di-GMP, and c-di-GMP binding by PlzA was found to be dependant upon arginine residues in the c-di-GMP binding region. Additionally, expression of PlzA was found to be upregulated by tick feeding and was constitutive in the mammalian host. We next constructed two deletion/allelic exchange mutants – one with the targeted deletion of PlzA, and on ethat replaced PlzA with PlzB in a strain lacking the plzB gene. Our studies demonstrated that ΔplzA was deficient in motility and was also non-infectious in the mouse model of B. burgdorferi infection. Additionally, this strain remained viable in larval Ixodes ticks. Also, B31-plzB KI was deficient in motility, as well as infectivity, demonstrating that PlzB is unable to complement for functions fo PlzA in vitro and in vivo and that it may play other roles in the biology of B. burgdorferi strains carrying the plzB gene. These studies represent the first identification of a c-di-GMP binding protein in any spirochete, but also represent the first demonstration of the importance of PilZ domain proteins in a spirochetal system. We additionally examined the effects of c-di-GMP synthesis and breakdown in the related bacterium, B. hermsii, a causative agent of tick-borne relapsing fever (TBRF). Deletion mutants in Rrp1 (B. hermsii’s sole diguanylate cyclase) and PdeA (B. hermsii’s only EAL domain-containing phosphodiesterase) were created. These strains were analyzed in order to determine: 1) the effect(s) of the losse of Rrp1/PdeA on intracellular spirochete c-di-GMP levels, and 2) the effects of Rrp1/PdeA on the establishment of murine infection and on gross motility/chemotaxis. It was demonstrated that c-di-GMP accumulates intracellularly in the cells lacking PdeA. Additionally, spirochetes were shown to chemotax towards N-acetyl-glucosamine (NAG) and they did not form soft agar swarms. In contrast, cells lacking Rrp1 did not accumulate detectable levels of c-di-GMP, demonstrated a reduced ability to chemotax towards NAG, and swarmed on soft agar in a fashion indistinguishable from wild type. Despite these differences in phenotype, both mutant strains display an attenuated murine infectivity. These results indicate that c-di-GMP is indeed important in the TBRF spirochete, B. hermsii and this vital second messenger plays key roles in virulence, motility, and chemotaxis. These studies also pave the way for future investigation of B. hermsii through use of targeted genetic manipulation.

Canada welcomes and supports refugees from all over the world. However, they encounter many struggles that force them to with withdraw from their English language classes. This Narrative Inquiry explores the lived experiences of two adult refugee students who could not continue with their English classes due to the hardships they encountered. Through research conversations I document their experiences in two narrative accounts, and read across them to find common threads. These common struggles include financial strains, employment, childcare, isolation and mental health. The findings point to the importance of further supporting refugee students in different ways. My recommendations are that ESL teachers develop lessons that include settlement, support services, and Canadian culture information. English language programs need to incorporate support services with professionals trained in ESL, and the different levels of government need to be part of the discussion on how to provide stronger supports through childcare, employment and information accessibility. / February 2016

In the South African context, it is currently assumed by most role players in education that teacher graduates are proficient in the medium of instruction they will use once they start their careers. This may be a reason why currently there is no mandatory testing of teachers‟ language proficiency. Due to social, economic and historical factors, English has become the medium of instruction at most educational institutions in South Africa, yet less than 10% of the population speak English as a home language. Consequently, an overwhelming number of learners are being taught in a language other than their home language and often by teachers not adequately prepared to teach through the medium of English. In the absence of mandatory testing and with the complex language situation in South African classrooms, this case study aimed to determine the perceptions held by pre-service teachers and their mentors regarding their English proficiency at entry to the teaching profession. This was done by answering the following research question: What is the perceived Classroom English proficiency of final year pre-service teachers prior to graduating? The conceptual framework was grounded in Uys‟s (2006) model of Classroom English proficiency and the methodology employed was quantitative in nature. Instruments used to collect data included an observation schedule completed by mentor teachers observing pre-service teachers presenting the fifth lesson of their first internship period, and a questionnaire completed by the pre-service teacher directly after the observed lesson. Both instruments were adapted from Elder‟s Classroom Language Assessment Schedule (1993, 2001), and each included 42 items related to various language proficiency variables rated on a four-point Likert scale. Data were analysed using various statistical measures comparing groups and subgroups within the sample. Key findings included a significant statistical difference between how English Home Language (EHL) pre-service teachers perceived their language proficiency and those who are English Additional Language (EAL) preservice teachers. EHL pre-service teachers perceived their Classroom English proficiency better than EAL pre-service teachers. The same trend was evident among the perceptions of mentor teachers. Pedagogical language and voice skills emerged as areas in which additional support was required by EAL pre-service teachers. Further research avenues to explore relate to the feasibility of administering refined instruments among teachers to determine not only what the perceptions would be on a national level but also to assess their Classroom English proficiency, followed by possible interventions. / Dissertation (MEd)--University of Pretoria, 2014. / lk2014 / Social Studies Education / MEd / Unrestricted

Augmentative and alternative communication (AAC) provides many individuals who have little or no functional speech with a means to enter the world of communication. Aided and/or unaided symbols are used as a means of reception and expression to create shared meaning. The selection of an appropriate symbol set/system is vital and iconicity plays a central role in this process. The Western-based symbol set, Picture Communication Symbols (PCS) (Johnson, 1981), is readily available and widely used in South Africa, despite little information existing on its iconicity to South African populations with disabilities. This study aimed to determine the iconicity of Picture Communication Symbols (PCS) for children with English Additional Language (EAL) and intellectual disability. A quantitative, non-experimental, descriptive design was used. Thirty participants between the ages of 12;00 and 15;11 (years; months) with EAL and intellectual disability were required to identify 16 PCS presented thematically on a ‘bed-making’ communication overlay in response to a gloss read out by the researcher. The results indicated that, overall, the 16 PCS were relatively iconic to the participants. The results also indicated that the iconicity of PCS can be manipulated and enhanced and that it can be influenced by other PCS that are used simultaneously on the communication overlay. The reasons for these findings are described. The clinical and theoretical implications of this study’s results are discussed, followed by a critical evaluation of this study and, finally, recommendations for future research are suggested. / Dissertation (Master of Arts)--University of Pretoria, 2012. / Centre for Augmentative and Alternative Communication (CAAC) / unrestricted

Sweden's first hospital, built in 1752, was Serafimerlasarettet on Kungsholmen in Stockholm. Since then, technological and medical developments have come a long way and so also the premises for medical care. Today, there is tough demands on flexibility and generality in hospital premises. The aim of this work is to create an understanding of local resource planning of hospital real estate in Stockholm. For instance what it looks like in practice, how it looks compared to the theory and the problems and possible solutions that are available on the specific local resource planning. Stockholm County Council is responsible for health care and dental care for residents of the county. The County Council is a politically driven organization with elections every four years. Politicians in committees and boards have the help of a number of administrations. Locum manages and develops care properties for the county council since 1991. Local resource planning for hospital buildings is very special. Questions regarding hospital premises are very complex and to coordinate health care for a large area like Stockholm County, with 1.9 million inhabitants, is not easy. Uncertainties and problems are always associated with local resource planning and hospital buildings are no exception. On the contrary, there are even more uncertainties. For example political incentives, the large organization, special environment, rapid technological development, demographic factors and the fact that health care is a sensitive issue for many. The county must find the right tools to manage this problem. The population is constantly increasing but at a faster pace than expected. This combined with the planning and implementation of expanding health care in a too late stage leads to difficulties. The Future plan for healthcare in Stockholm promises to be a well-made plan but it is far from finished and many questions remain. / Sveriges första sjukhus, från år 1752, var Serafimerlasarettet på Kungsholmen i Stockholm. Sedan dess har den tekniska och medicinska utvecklingen kommit långt och så även lokalerna för sjukvård. Idag är det hårda krav på flexibilitet och generalitet på sjukhusfastigheter. Syftet med detta arbete är att skapa förståelse kring lokalresursplaneringen av sjukhusfastigheter i Stockholm. Exempelvis hur arbetet faktiskt går till i praktiken, hur det ser ut jämfört med teorin samt vilka problem och eventuella lösningar det finns kring den specifika lokalresursplaneringen. Stockholms läns landsting ansvarar för hälso- och sjukvård samt tandvård för bosatta i Stockholms län. Landstinget är en politisk styrd organisation med val vart fjärde år. Politikerna i nämnder och styrelser har hjälp av ett antal förvaltningar. Locum förvaltar och utvecklar vårdfastigheter för landstinget sedan 1991. Lokalresursplanering för sjukhusfastigheter är väldigt speciell. Lokalfrågorna är mycket komplexa och att samordna sjukvården för ett stort område som Stockholms län, med 1.9 miljoner invånare, är inte lätt. Osäkerheter och problem är alltid förknippat med lokalresursplanering och sjukhusfastigheter är inget undantag utan tvärt om, där finns här ännu fler. Politiska incitament, den stora organisationen, speciell miljö, snabb teknisk utveckling, demografiska faktorer och det faktum att sjukvård är en känslig fråga för många är exempel på detta. Stockholms län måste hitta rätt verktyg för att hantera denna problematik. Befolkningsmängden ökar kontinuerligt men med en snabbare fart än beräknat. Detta i kombination med att planeringen och genomförandet av att utöka sjukvården kommer i ett allt för sent skede, resulterar i svårigheter. Framtidens plan för hälso- och sjukvården i Stockholms län ser ut att vara en välutarbetad plan men den är långt ifrån klar och många frågetecken kvarstår.

Sweden is seen as a successful adopter of English as an additional language (EAL) and the country is a frontrunner in the globalization of the English language. Much of the success could be attributed to the large presence of English in Swedish society. Additionally, EAL learners are acquiring the English language out-of-school to a larger extent, often referred to as Extramural English (EE). Therefore, it is important to investigate how EE affects learners, teachers and discuss how it can change education. This has been done by evaluating the current state of research through a systematic literature review. It was found that learners engage in a variety of EE activities, with playing online games and watching movies being the activities with the most English exposure. Learners engage in these activities mostly out of their own interests or because of other socially driven motives. EE affects their opinion of English and could have negative effects on their attitude towards school English. Teachers are aware of the gap between EE and school English and try to integrate activities similar to EE activities in class. However, it is a challenge to find authentic material that fits a large number of learners’ interests. In conclusion, there is a need for more research that could point to a clear cause-and-effect relationship between EE and high proficiency in English. This thesis also calls on teachers, principals, and school leaders to prepare to change education if EE becomes a more widespread phenomenon.

Legionella pneumophila est un pathogène intracellulaire, agent de la Légionellose, et dont le réservoir dans l’environnement est constitué d’amibes aquatiques comme Acanthamoeba castellani. Mes objectifs de thèse étaient l’identification de mécanismes moléculaires contrôlant la virulence et la multi-résistance chez L. pneumophila, et en particulier l’exploration du rôle des protéines « GGDEF/EAL ». Les domaines GGDEF et EAL sont retrouvés dans des enzymes permettant respectivement de synthétiser (diguanylate cyclase, DGC) ou dégrader (phosphodiestérase, PDE) le di-GMP cyclique, un second messager spécifique des bactéries, qui participe au contrôle de fonctions clés comme la virulence ou la mobilité. L. pneumophila Lens possède 22 gènes codant des protéines GGDEF/EAL, et dont la plupart sont exprimés lorsqu’elle est dans sa phase virulente. L’activité enzymatique des 22 protéines « GGDEF/EAL » a été analysée in vitro : sur 10 protéines purifiées, 6 sont des DGC, dont 2 présentes une double activité DGC/PDE. L’inactivation de 5 gènes des 22 gènes et la surexpression de 2 autres entrainent une baisse de la virulence vis-à-vis d’A. castellanii. De plus, nous avons montré que l’activité DGC d’au moins 2 de ces protéines est requise lors du cycle infectieux. Enfin, nous avons décrit un système à deux composants original comprenant l’histidine kinase (HK) Lpl0330, capable de s’autophosphoryler sur un nouveau domaine HisKA, retrouvé dans 64 autres HK potentielles, et Lpl0329, le premier régulateur de réponse à double activité enzymatique caractérisé, dont la phosphorylation conduit à moduler le taux de di‐GMPc en favorisant une de ses deux activités (Levet-Paulo et al., 2011). / Legionella pneumophila is an intracellular pathogen found in aquatic environments where it replicates in protozoan hosts. My objectives were to identify molecular mechanisms that control virulence and multidrug resistance in L. pneumophila, and especially to explore the role of proteins “GGDEF/EAL” in virulence. GGDEF and EAL domains are found in enzymes able to synthesize (diguanylate cyclase, DGC) or degrade (phosphodiesterase, PDE) c-di-GMP, respectively. C-di-GMP is a bacterial second messenger which plays a key role in regulating main functions including motility, and virulence. L. pneumophila Lens contains 22 genes encoding GGDEF/EAL proteins and most of them are expressed simultaneously with genes encoding virulence factors. The enzymatic activities of the 22 GGDEF/EAL proteins of L. pneumophila Lens were assayed in vitro. Among the 10 proteins purified, 6 showed a DGC activity and 2 contained both activities. The role of the GGDEF/EAL proteins of L. pneumophila Lens on virulence was investigated. Inactivation of 5 genes and overexpression of 2 other genes led to a significant decrease in virulence. Moreover, DGC activity of at least two of these proteins is required for bacterial virulence. Finally, an original two-component system was identified comprising Lpl0330, a histidine kinase able to autophosphorylate on a new HisKA domain, and Lpl 0329, a protein with dual in vitro DGC/PDE activity. Phosphorylation of Lpl0329 led to a decrease in its DGC activity only, giving the first example of a bifunctional enzyme which modulates synthesis and turnover of c-di-GMP in response to phosphorylation (Levet-Paulo et al., 2011).

c-di-GMP stellt einen wichtigen Regulator bei der Kontrolle von Motilität, Virulenz und der Biofilmbildung in Bakterien dar. Aufgrund der Vielfalt c-di-GMP-synthetisierender (Diguanylatzyklasen, DGC) bzw. abbauender (Phosphodiesterasen, PDE) Enzyme, c-di-GMP-bindender Effektoren und zellulärer Antworten etablierte sich die Theorie paralleler Regulationsmodule, die sich aus DGC, PDE, Effektor und Zielmolekül zusammensetzen und spezifische Prozesse wie die Cellulose-Synthese kontrollieren. Während die Aktivierung dieser durch c-di-GMP bereits verstanden und damit Effektor und Zielmolekül bekannt sind, konnten dem Modul bisher keine spezifisch wirkenden DGCs und PDEs zugeordnet werden. Im Rahmen dieser Arbeit konnte gezeigt werden, dass die Proteine DgcC und PdeK in E. coli spezifisch auf die Cellulose-Synthese in Makrokolonie-Biofilmen wirken und ein solches c-di-GMP-Modul bilden. Beide sind aktiv an der Umsetzung von c-di-GMP beteiligt und mittels Interaktionen Teil eines Multi-Protein-Komplexes, der auch die Cellulose-Synthase-Einheiten BcsA und BcsB umfasst. Aufgrund dieser Co-Lokalisation können DgcC und PdeK die c-di-GMP-Konzentration in unmittelbarer Umgebung zum c-di-GMP-bindenden Effektor BcsA kontrollieren. DgcC-PdeK somit stellen das erste Regulationsmodul dar, welches durch lokalisierte Synthese und Abbau von c-di-GMP wirkt und dessen Spezifität aufgrund multipler Protein-Interaktionen gewährleistet wird. 2011 kam es in Mitteleuropa zu einem schweren Ausbruch von Shiga-Toxin-produzierenden E. coli O104:H4, in dessen Verlauf fast 4000 Menschen erkrankten und etwa 20% ein Hämolytisch-urämisches Syndrom entwickelten. Die in dieser Arbeit erlangten Ergebnisse legen nahe, dass die einzigartige Kombination aus Toxin-Produktion und Biofilm-assoziierter Eigenschaften – das Potential zur c-di-GMP-Akkumulation (TL Povolotsky), verstärkte CsgD-Synthese und vor allem keine Cellulose-Produktion– möglicherweise zur erhöhten Virulenz dieses E. coli O104:H4 beitragen. / c-di-GMP represents an important regulator in the control of motility, virulence and biofilm formation. Due to the multiplicity of c-di-GMP-forming (diguanylate cyclases, DGCc) and -degrading (phosphodiesterases, PDEs) enzymes, c-di-GMP-binding effectors and cellular outputs, the theory of parallel existing c-di-GMP-regulation modules was established. Such a module consists of a DGC, a PDE, an effector and a target controlling a specific cellular output such as cellulose synthesis. Whereas the activation of cellulose synthesis is well understood, and therefore effector and target molecule are known, so far no DGC or PDE has been associated with the cellulose-specific c-di-GMP-module. Within the framework of this work it was shown that DgcC and PdeK act specifically on the regulation of cellulose synthesis in macrocolony biofilms, thus forming a c-di-GMP module. Both proteins are enzymatically active concerning c-di-GMP metabolism and through protein-interactions part of a multi-protein-complex, which includes the cellulose synthase-subunits BcsA and BcsB, too. Due to this co-localisation DgcC and PdeK can control the c-di-GMP-concentration in close proximity to the c-di-GMP-binding cellulose-synthase BcsA. Therefore, DgcC-PdeK represents the first signalling module, which acts through local c-di-GMP-synthesis and -degradation and controls specifically cellulose because of multiple protein-interactions with the synthase-complex. In 2011 a serious outbreak of shiga toxin producing E. coli O104:H4 occurred in Middle Europe with nearly 4000 patients of whom approximately 20% developing haemolytic uraemic syndrome. The results obtained in this study suggest that a unique combination of shiga toxin production and biofilm-associated properties – the potential of c-di-GMP accumulation (see PhD Thesis T. L. Povolotsky), enhanced CsgD-synthesis at 37°C and especially no cellulose production – potentially contribute to the enhanced virulence of E. coli O104:H4.

Legionella pneumophila est une bactérie aquatique qui prolifère en se répliquant à l’intérieur de cellules amibiennes. Elle peut persister dans ces environnements en vivant en communauté sous forme de biofilms. L’inhalation par l’Homme d’eau contaminée, vaporisée par les réseaux d’eau chaude ou les tours aéro‐réfrigérantes, peut mener à l’infection des macrophages pulmonaires qui se traduit par une grave pneumonie appelée légionellose. Le di‐GMP cyclique (diGMPc) est impliqué, chez diverses espèces bactériennes, dans la transition entre les modes de vie mobiles et sessiles, et chez certains pathogènes, dans la régulation de la virulence. Mon travail de thèse vise à démontrer l’implication des voies de signalisation à diGMPc dans le contrôle de la virulence et de la formation de biofilms par L. pneumophila. Cette implication a été étudiée grâce à l’inactivation systématique de chacun des gènes codant les protéines du métabolisme du diGMPc chez la souche L. pneumophila Lens. Notre étude a révélé que trois de ces protéines, Lpl0780, Lpl0922 et Lpl1118, sont spécifiquement requises pour le contrôle de la virulence et, plus particulièrement, pour la survie précoce lors de l’infection de cellules‐hôtes via l’orchestration de la sécrétion de facteurs de virulence dans la cellule‐hôte. Lpl1118 participerait également à la biogénèse de la vacuole de réplication. Cinq autres de ces protéines sont impliquées dans la régulation de la formation et de l’architecture des biofilms. L’une d’elles est, plus particulièrement, requise pour la formation de biofilms en présence d’oxyde nitrique. Ces résultats contribuent à une meilleure compréhension de l’organisation complexe et spécifique des voies de signalisation à diGMPc chez L. pneumophila et pourraient permettre d’envisager une lutte plus efficace contre ce pathogène / Legionella pneumophila is a bacterium that proliferates in fresh water environments through the replication within amoebas. These bacteria can persist in these environments through biofilm formation. The inhalation of aerosolized contaminated water through hot water systems or cooling towers can induce the infection of human lungs, leading to a severe pneumonia called legionellosis. Cyclic di‐GMP (c‐di‐GMP) in involved, in various bacterial species, in the motility‐to‐sessility transition, and in some pathogens, in virulence control. My work aims to demonstrate the involvement of signaling pathways that use c‐di‐GMP in virulence control and biofilm formation of L. pneumophila. This involvement was investigated by systematically inactivating each gene encoding a c‐di‐GMP‐metabolizing enzyme in L. pneumophila Lens strain. Our work revealed that 3 of these proteins, Lpl0780, Lpl0922 and Lpl1118 are specifically involved in virulence control and, particularly, in the early survival during host cell infection through the orchestration of virulence factors secretion within host cell. Lpl1118 is particularly required for replicative vacuole biogenesis. Five other proteins, participate in the formation and architecture of biofilms. One of them is more specifically involved in biofilm formation in the presence of nitric oxide. These results help to better understand the complexity and the specificity of c‐di‐GMP signaling pathways in L. pneumophila and should allow the exploration of more effective ways to fight this pathogen