published_or_final_version / Architecture / Master / Master of Landscape Architecture

Master of Science / Department of Animal Science / R. Scott Beyer / Daniel Y.C. Fung / Clostridium perfringens causes necrotic enteritis (NE), resulting in decreased feed efficiency and increased mortality, costing the poultry industry USD 2 billion a year worldwide. The objective of the first trial was to validate the Fung Double Tube (FDT) to detect and enumerate C. perfringens in chicken intestines. Two methods (FDT and petri plates) and three media (Shahidi Ferguson Perfringens [SFP] with egg supplement, polymyxin B [p], and kanamycin [k; E]; SFP with p and k [P]; and SFP with cycloserine [C]) were arranged in a 2 x 3 factorial, resulting in six treatments. The FDT with medium C (5.35 log CFU/g) had significantly (P<0.05) higher C. perfringens counts than any other media/method combination. The objective of the second and third trials was to determine the effect of diet type on the population of C. perfringens in broiler intestines using the FDT. Trial 2 tested: corn-soybean meal (SBM), low-crude protein (19.8%)/high synthetic amino acids (SAA), and barley (56%)-fishmeal (4%; BF). Diets in Trial 3 included: corn-SBM, barley (7.46%), fishmeal (4%), and BF. Diets in Trial 2 contained an antibiotic and a coccidiostat; diets in Trial 3 did not. After 21 days, birds in Trial 2 fed BF had significantly higher (P<0.05) counts (5.96 log CFU/g) of C. perfringens, as compared to all other diets. Both, corn-SBM and SAA diets resulted in 3.89 log CFU/g. In Trial 3, birds fed the corn-SBM diet (2.7 log CFU/g) had significantly lower (P<0.05) counts than broilers fed BF (4.15 log CFU/g). When broilers were fed fishmeal (3.583 log CFU/g) and barley (3.577 log CFU/g) separately, C. perfringens counts were numerically higher compared to the corn-SBM diet, but numerically lower than birds fed BF. Barley and fishmeal inclusion increased the incidence of C. perfringens, and their combination resulted in a cumulative effect. The FDT method is able to detect C. perfringens at higher levels than the conventional petri plate method (P<0.001) and it also proved to be an effective method to detect differences in C. perfringens counts from the intestines of chickens fed different diet.

This study aimed to investigate the association of three species of arbuscular mycorrhizal (AM) fungi (Rhizophagus intraradices; Gigaspora albida and Claroideoglomus etunicatum) with J. curcas, and the effect this symbiosis in developing pre-mycorrhizal seedlings submitted to salt stress at home vegetation. Two experiments were conducted, the first to investigate the association of the fungus to the plant, in a completely randomized design with five treatments (control, isolated species and three inoculated together-MIX) and six repetitions. The second trial was completely randomized blocks in a factorial 4 x 2, with four levels of NaCl (2, 5, 8 and 10 dS / m), and presence or absence of mycorrhizae (MIX), which were evaluated mycorrhizal dependency (MD) and the development of seedlings in salinity soil. The three species of mycorrhizal fungi are associated with J. curcas both in isolation, as when inoculated together. The interaction between these factors showed that the control plants did not differ presence of mycorrhizae, whereas in the saline treatments, pre-mycorrhizal seedlings grew better than non-inoculated. The MD was higher in saline treatments and moderate stress (5 dS / m) provided the best results for this index. Salinity reduced the size of plants, photosynthetic performance, the photosynthetic pigment content, the amount of calcium, potassium, phosphorus and magnesium in the leaves and increased absorption of sodium and chlorine, lipid peroxidation, the activity of antioxidant enzymes and the concentration of proline. Pre-mycorrhizal plants had higher mass, height, stem diameter, number of leaves, leaf area, area ratio pillar, specific leaf area, reduced biomass allocation in leaves and larger, at root, increased root / shoot , higher photosynthetic rate and pigment content, lower activity of antioxidant enzymes, lipid peroxidation reduction, and increased proline content compared to plants not inoculated in the same saline conditions. In seedlings with AMF, high photosynthetic rate, coupled with the large amount of salts in the leaves, the significant increase in proline content and the absence of lipid peroxidation indicates a mechanism mitigating the effect of salinity associated with the presence of mycorrhizae. Therefore, J. curcas seedlings previously mycorrhizal are an alternative to the cultivation of the species under salt stress. / O objetivo deste estudo foi verificar a associação de três espécies de fungos micorrízicos arbusculates (FMAs) (Rhizophagus intraradices; Gigaspora albida e Claroideoglomus etunicatum) com Jatropha curcas L., e o efeito desta simbiose no desenvolvimento de mudas pré-micorrizadas, submetidas a estresse salino, em casa de vegetação. Foram realizados dois experimentos, sendo o primeiro para verificar a associação dos fungos à planta, em delineamento inteiramente casualizado, com cinco tratamentos (controle, espécies isoladas e as três inoculadas em conjunto-MIX) e seis repetições. O segundo experimento foi em blocos inteiramente casualizados, num esquema fatorial 4 x 2, com quatro níveis de NaCl (2, 5, 8 e 10 dS / m), e presença ou não de micorrizas (MIX), onde foram avaliados a dependência micorrízica (DM) e o desenvolvimento das mudas em solo salinizado. As três espécies de FMAs se associam a J. curcas tanto de forma isolada, como quando inoculadas em conjunto. A interação entre os fatores mostrou que plantas do tratamento controle não diferem quanto presença de micorrizas, enquanto que, nos tratamentos salinos, as mudas pré-micorrizadas se desenvolveram melhor que as não micorrizadas. A DM foi maior nos tratamentos salinos e o estresse moderado (5 dS / m) proporcionou o melhor resultado para este índice. A salinidade reduziu o tamanho das plantas, o desempenho fotossintético, o teor de pigmentos fotossintéticos, a quantidade de cálcio, potássio, fósforo e magnésio nas folhas, e aumentou a absorção de sódio e cloro, a peroxidação de lipídios, a atividade das enzimas antioxidantes e a concentração de prolina. As plantas pré-micorrizadas apresentaram maior massa, altura, diâmetro do coleto, número de folhas, área foliar, razão de área filar, área foliar específica, menor alocação de biomassa nas folhas e maior, na raiz, aumento da relação raiz/parte aérea, maior taxa fotossintética e teor de pigmentos, menor atividade de enzimas antioxidantes, redução da peroxidação lipídica e aumento no teor de prolina em relação às plantas não micorrizadas, nas mesmas condições salinas. Nas mudas com FMAs, a alta taxa fotossintética, associada a grande quantidade de sais nas folhas, ao aumento expressivo do teor de prolina e a ausência da peroxidação de lipídios indica que há um mecanismo mitigador do efeito da salinidade associado à presença de micorrizas. Portanto, mudas de J. curcas previamente micorrizadas são uma alternativa para o cultivo da espécie sob estresse salino.

Thesis (M. Hous. M.)--University of Hong Kong, 1999. / Includes bibliographical references (leaves 82-85).

Thesis (M.Hous.M.)--University of Hong Kong, 1999. / Includes bibliographical references (leaves 82-85). Also available in print.

綜觀1997年亞洲金融風暴之後的泰國，泰國政府設法改善以農立國的機會點，運用大量農產品輸出提升國家整體經濟，這其中最為驚人的成就便是世界廚房的執行，政府透由單一窗口的溝通，明定清楚的營運績效指標，讓泰式料理躍上舞台成為關鍵字的搜尋，也成功的吸引無數的觀光旅客前往泰國一窺究竟，深入探訪學習泰式料理與體驗泰國文化。然而在看了別人的成功經驗之後，台灣的餐飲業是否有機會可以學習呢？因此本研究試圖由相關文獻與資料的蒐集，並深入訪問在餐飲領域學有所長的專家意見，藉由泰國政府世界廚房的營運經驗中找出成功關鍵，進而反思台灣餐飲業可以學習的作為，當中也加上中小企業鼎泰豐的營運方式說明，並引用了白地策略的商業模式思考我們日後可以運作的方法與營運法則。 經過這一連串的研究探討，茲將歸納總結出三方向的建議 一. 政府端：建議政府單位能夠仿效學習成立專責單位，儘可能簡化作業時間與文書往返的營運模式，讓權責單位能夠更具效能的執行餐飲業的推廣；再者近來的食品加工風波，亦是政府單位需要改善制度，設法成立食材履歷的改善作為，好讓餐飲業的經營除了美味更具安心。同時我們透由泰國世界廚房的營運經驗也可以發現，維護品質的稽核制度不可少。 二. 企業或組織端：透由連鎖化的營運模式壯大自己的營運成績，讓自己形塑品牌深化才能有機會在日益變遷的餐飲環境中紮根。再者應該結合台灣科技化的優勢，架構資訊平台讓營運的經驗法則能夠累積成自己的創新價值，形成關鍵流程與資源，讓自己的營運評斷基準更具說服力。 三. 顧客端：日益成熟的台灣社會，我們更應該努力成為有同理心的消費者，在購賣決策形成時，除了廉價思維之外，更應該思考商品的附加價值，讓整體的經營環境，不光只有法令、業者的努力，更有許多消費者的認可，才能讓餐飲業的發展朝光明面邁進。 關鍵詞：泰國世界廚房、白地策略、鼎泰豐、個案研究、餐飲業 / After Asian financial crisis, Thailand’s government tried to improve the department of agriculture in1997 and found the opportunity. They increased the economic growth by exporting the product of agriculture. The most of achievements is the implement of the Center for Thailand kitchen of the world. What can we learn from f the Center for Thailand kitchen of the world？What are the major reasons of their achievement? This paper will use the literature review and interview expert of restaurant industry. We also choose Din Tai Fung to be our case study and use the rules of white space. After our empirical study, we have three conclusions: 1. We thought that our government can reduce the process of paper work and provide the organization of one stop service. After the crisis of food processing，we can set up the food pedigree to improve the quality of food & beverage. According the business model of Center for Thailand kitchen of the world, we need to build the system of quality auditing. 2. The franchise system can expand the scale of business. Through the franchise model, the enterprise will have the opportunity to increase the market share in the food & beverage industry. We have the advantages of hi-tech technology in Taiwan. We can run the successful business model by accumulating these experiences on web site. 3. We should follow our consumer’s preference. On the consumption decision, we can try to add value on the product. We also need to build the suitable environment and law. Keywords：Center for Thailand kitchen of the world、White Space、Din Tai Fung、Case study、Food & Beverage Industry )

No description available.