Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-03-02T17:30:01Z No. of bitstreams: 5 8.pdf: 681231 bytes, checksum: aba8d63151d3cca70987cd244a84bf30 (MD5) license_text: 20626 bytes, checksum: 7851d446050a985bd0603b7c4cee472d (MD5) license_rdf: 19710 bytes, checksum: aa65da15f424ff71c620a992a187295c (MD5) license_url: 49 bytes, checksum: fd26723f8d7edacdb29e3f03465c3b03 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-03-02T17:39:11Z (GMT) No. of bitstreams: 5 8.pdf: 681231 bytes, checksum: aba8d63151d3cca70987cd244a84bf30 (MD5) license_text: 20626 bytes, checksum: 7851d446050a985bd0603b7c4cee472d (MD5) license_rdf: 19710 bytes, checksum: aa65da15f424ff71c620a992a187295c (MD5) license_url: 49 bytes, checksum: fd26723f8d7edacdb29e3f03465c3b03 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-03-02T17:39:11Z (GMT). No. of bitstreams: 5 8.pdf: 681231 bytes, checksum: aba8d63151d3cca70987cd244a84bf30 (MD5) license_text: 20626 bytes, checksum: 7851d446050a985bd0603b7c4cee472d (MD5) license_rdf: 19710 bytes, checksum: aa65da15f424ff71c620a992a187295c (MD5) license_url: 49 bytes, checksum: fd26723f8d7edacdb29e3f03465c3b03 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2009 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / As sempre-vivas (Syngonathus spp) esp?cie pertencente ? fam?lia Eriocaulaceae, possui grande interesse comercial e recentemente medicinal para a popula??o da sua ?rea de ocorr?ncia. Com a inclus?o de algumas esp?cies desse g?nero na lista de esp?cies amea?adas de extin??o torna-se necess?rias medidas que garantam a conserva??o da esp?cie seja in situ ou ex situ. Diante disso, a crioconserva??o de sementes ? uma alternativa economicamente vi?vel e que exige um protocolo para cada esp?cie. Dessa forma, o objetivo do presente trabalho foi avaliar a qualidade fisiol?gica de sementes de sempre-viva em rela??o ao efeito do descongelamento ap?s imers?o em nitrog?nio l?quido e a influ?ncia dos diferentes locais e per?odos de armazenamento. A pesquisa foi dividida em duas partes. A primeira parte consistiu em avaliar o efeito do descongelamento em quatro ambientes (freezer, ambiente,banho-maria e microondas) sobre o comportamento fisiol?gico das sementes de sempre-viva Syngonanthus elegans e Syngonanhus arthrotrichus. Os tratamentos foram dispostos em com delineamento inteiramente casualizado e os dados foram submetidos ? an?lise de vari?ncia, com as m?dias comparadas entre si pelo teste de Tukey a 5% de probabilidade. Pelos resultados constatou-se que ambas as esp?cies podem ser crioconservadas. Para as Syngonanthus elegans n?o houve diferen?a estat?stica entre os tratamentos e para a Syngonanthus arthrotrichus o descongelamento em microondas e ? temperatura ambiente proporcionaram os melhores resultados. A segunda parte consistiu em avaliar os locais de armazenamento (nitrog?nio l?quido, geladeira e ambiente) durante quatro per?odos (0, 40, 80 e 120 dias) atrav?s dos testes de vigor (teste de primeira contagem da germina??o e ?ndice de velocidade de germina??o), germina??o, mat?ria fresca, mat?ria seca e comprimento de pl?ntulas. Os tratamentos foram dispostos em arranjo fatorial (3X4), com delineamento inteiramente casualizado e os dados foram submetidos ? an?lise de vari?ncia, com as m?dias comparadas entre si pelo teste de Tukey a 5% de probabilidade. Pelos resultados constatou-se que esta esp?cie pode ser crioconservada com sucesso e que todos os m?todos de descongelamento em temperatura ambiente e em microondas consistiram na melhor alternativa. O armazenamento em geladeira apresentou os melhores resultados para as sementes analisadas. Para todos os par?metros avaliados todos os per?odos de armazenamento foram eficientes para as sementes de S. elegans. As sementes de Syngonanthus arthrotrichus mantiveram a qualidade fisiol?gica quando armazenadas em geladeira. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2009. / ABSTRACT The Sempre-viva (Syngonathus spp.) Species belonging to the family Eriocaulaceae, has great commercial interest for medical and recently the people of their area of occurrence. With the inclusion of some species of this genus in the list of endangered species it is necessary measures to ensure the conservation of the species is in situ or ex situ. Thus, the cryoconservation seed is a viable alternative and requires a protocol for each species. Thus, the purpose of this study was to evaluate the physiological quality of seeds of sempre-viva on the effect of thawing after immersion in liquid nitrogen and the influence of different places and periods of storage. The research was divided into two parts. The first part was to assess the effect of thawing in three environments (freezer, environment and water bath) on the physiological behavior of seeds of sempre-viva Syngonanthus elegans and the effect of the period (0, 40, 80 and 120 days) under the local storage (liquid nitrogen, refrigerator and environment), through tests of force test (first count of the germination rate and speed of germination), germination, green mass, dry weight and length of seedlings. Treatments were arranged in a factorial arrangement, with a randomized design and data were submitted to analysis of variance, compared with the averages among themselves by Tukey test at 5% probability. By the results it was found that this species can be successfully criokept and that all methods of thawing studied in this work, may be used. The research was divided into two parts. The second part was to assess the effect of thawing in three environments (freezer, environment and water bath) on the physiological behavior of seeds of sempre-viva Syngonanthus arthrotricus and the end of the period (0, 40, 80 and 120 days) under the local storage (liquid nitrogen, refrigerator and environment), through tests of force test (first count of the germination rate and speed of germination), germination, green mass, dry weight and length of seedlings. Treatments were arranged in a factorial arrangement, with a randomized design and data were submitted to analysis of variance, compared with the averages among themselves by Tukey test at 5% probability. By the results it was found that this species can be successfully criokept, and all the methods of thawing at room temperature and microwave were in the best alternative. The store in freezer showed the best results for seeds analyzed .

Made available in DSpace on 2016-04-28T14:58:34Z (GMT). No. of bitstreams: 1 2005- Cristiane Miranda Martins.pdf: 5016347 bytes, checksum: edbb51eb103817d7b3f8ed06497e9daa (MD5) Previous issue date: 2005-02-18 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The present work was carried out at UFRRJ, in Serop?dica, Rio de Janeiro state, to study the influence of tegument and aril on the germination of seeds from two Passifloraceae species: Passiflora edulis f. flavicarpa Deg. and Passiflora alata Curtis. obtained from whole market fruits pur chased at CEASA-RJ. On the first stage the seeds were evaluated in regard to the physiological and sanitary quality at different substrates and temperatures, as well as to the the process of imbibition and hydric restrition. A three-phase pattern of seed imbibition, was observed, germination decreased from the osmotic potential of -0,6 MPa and increased in sand at the temperature alternating from 20 to 30? C in both species. However, sanitary quality was greatest in fungicide treated seeds with removed aril. The results have pointed out that the seeds from both species presented highest percentage of germination upon aril removal, regardless the seed extremity cutting-off. On the second stage seed extracts were obtained with organic solvents of different polarities. These extracts were identified by classes and a sensibility test was performed with Lactuca sativa L. seeds. Steroids and triglicerides were detected in the dichloromethane extract, carbohydrates were detected in methanol extract. Lettuce seeds germination was inhibited by the dichlorinemethanol extract and increased the members of abnormal seedlings and hard seeds. / O presente trabalho foi realizado na UFRRJ, em Serop?dica, RJ, com a finalidade de estudar a influ?ncia do tegumento e do arilo na germina??o de sementes de duas esp?cies de Passifloraceas, Passiflora edulis f. flavicarpa Deg. e Passiflora alata Curtis., obtidas de frutos oriundos de lotes comerciais, adquiridos no CEASA RJ. Na primeira etapa, as sementes foram avaliadas quanto ? qualidade fisiol?gica e sanit?ria, em diferentes substratos e temperaturas, bem como o processo de embebi??o e de restri??o h?drica, nos quais se constatou o modelo trif?sico de embebi??o das sementes, redu??o da germina??o a partir do potencial 0,6 MPa, melhoria da germina??o em substrato areia e na temperatura alternada de 20-30?C, em ambas as esp?cies. Contudo, a qualidade sanit?ria foi maior nas sementes onde foi aplicado fungicida e onde o arilo havia sido removido. Os resultados indicaram que sementes de ambas as esp?cies apresentaram maior porcentagem de germina??o na aus?ncia do arilo, independente do desponte. Na segunda etapa foram obtidos extratos com solventes org?nicos de diferentes polaridades, identificadas ?s classes de subst?ncias presentes e realizado teste de sensibilidade dos mesmos com sementes de Lactuca sativa L. Foram detectados no extrato obtido com diclorometano triglicer?deos e ester?ides; e carboidratos no extrato metan?lico. O extrato diclorometano inibiu a germina??o de sementes de alface, aumentando o n?mero de pl?ntulas anormais e sementes duras, indicando que as subst?ncias contidas nesse extrato influenciaram o processo germinativo.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2017-10-31T00:53:19Z No. of bitstreams: 1 Marisol Ferraz - tese final.pdf: 2927112 bytes, checksum: e8aefa039b3e565bd665b7e168e19f5d (MD5) / Made available in DSpace on 2017-10-31T00:53:19Z (GMT). No. of bitstreams: 1 Marisol Ferraz - tese final.pdf: 2927112 bytes, checksum: e8aefa039b3e565bd665b7e168e19f5d (MD5) Previous issue date: 2016-12-19 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The caatinga is a biome with great biological diversity, endemism and big potential for the production of bioactive compounds of economic interest, but with a great lack of information about the vegetal species and their forms of conservation. This work in order to reduce this gap and ensure their genetic conservation, eight species of subtribe Hyptidinae (Lamiaceae): Eplingiella fruticosa (Salzm. ex Benth.) Harley & J.F.B.Pastore, Gymneia platanifolia (Salzm. ex Benth.) Harley & J.F.B.Pastore, Hyptis lanceolata Poir., Hyptis ramosa Pohl ex Benth., Hyptis velutina Pohl ex Benth., Martianthus leucocephalus (Salzm. ex Benth.) J.F.B.Pastore, Mesosphaerum pectinatum (L.) Kuntze, Mesosphaerum suaveolens (L.) Kuntze., the seeds was characterized, the mucilage was extracted and the storage was done for twenty-four months in four different environments: 10 ? C without humidity control, to 10 ? C and 25 ? C with zinc chloride saturated solution, in liquid nitrogen at -196 ? C. The germinative response was tested every three months to evaluate storage efficiency in seed conservation. The efficiency of the use of X-ray image in the evaluation of the quality of the stored seeds was tested. The moisture content of the seeds ranged from 1.98% to 8.05%, suitable for storage. E. fruticosa, M. pectinatum and M. suaveolens can be good sources of mucilage due to their high yield. The environments with low relative humidity (5.5%) maintained by zinc chloride were efficient in the storage of the seeds of five of the eight evaluated species. X-ray image analysis is effective in evaluating seed quality. / A caatinga ? um bioma com uma grande diversidade biol?gica, endemismos egrande potencial para produ??o de compostos bioativos de interesse econ?mico, por?m com uma grande car?ncia de informa??es sobre as esp?cies vegetais e suas formas de conserva??o.Neste trabalho, com o objetivo de diminuir essa car?ncia e garantir sua conserva??o gen?tica, oito esp?cies da subtribo Hyptidinae (Lamiaceae):Eplingiella fruticosa (Salzm. ex Benth.) Harley & J.F.B.Pastore, Gymneia platanifolia (Salzm. ex Benth.) Harley & J.F.B.Pastore, Hyptis lanceolata Poir., Hyptis ramosa Pohl ex Benth.,Hyptis velutina Pohl ex Benth.,Martianthus leucocephalus (Salzm. ex Benth.) J.F.B.Pastore, Mesosphaerum pectinatum (L.) Kuntze, Mesosphaerum suaveolens (L.) Kuntze., tiveram as sementes caracterizadas, foi feita a extra??o da mucilagem e o armazenamento por vinte e quatro meses em quatro ambientes distintos: 10?C sem controle de umidade, a 10?C e 25?C com solu??o saturada de cloreto de zinco, e em nitrog?nio l?quido a -196 ?C. A cada tr?s meses foi testada a resposta germinativa para avaliar a efici?ncia do armazenamento na conserva??o das sementes. Foi testada a efic?cia do uso de imagem de raio X na avalia??o da qualidade das sementes armazenadas.O teor de umidade das sementes variou de 1,98% a 8,05%, adequados ao armazenamento.E. fruticosa, M. pectinatum e M. suaveolens podem vir a ser boas fontes de mucilagem por apresentar alto rendimento.Os ambientes com baixa umidade relativa (5,5%) mantida pelo cloreto de zinco se mostraram eficientes no armazenamento das sementes de cinco das oito esp?cies avaliadas mantendo a viabilidade durante o per?odo avaliado. A an?lise da imagem de raio X ? eficaz na avalia??o da qualidade das sementes.

Submitted by Jadson Francisco de Jesus SILVA (jadson@uefs.br) on 2018-01-26T21:53:28Z No. of bitstreams: 1 disserta??o Eliza 14 de dezembro final.pdf: 1828093 bytes, checksum: 7d102752271132d8745e82e9533c13b3 (MD5) / Made available in DSpace on 2018-01-26T21:53:28Z (GMT). No. of bitstreams: 1 disserta??o Eliza 14 de dezembro final.pdf: 1828093 bytes, checksum: 7d102752271132d8745e82e9533c13b3 (MD5) Previous issue date: 2017-07-14 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The stress situation in Caatinga is aggravated by climate changes, from which are predicted higher temperatures; water scarcity and higher soil salinization. Studies evaluating differences in seeds tolerance to abiotic stress among samples or genotypes of the same species can constitute physiologic tools and significant and complementary information for the selection of the most adapted group to environmental pressures. This work aimed to compare the seed vulnerability of different samples of Erythrina velutina (Fabaceae) to abiotic stress; evaluate que physiologic quality of seeds and determine the tolerance limits to saline, hydric and thermic stress. The assays were conducted at EMBRAPA semiarido Seeds laboratory and at the Germination laboratory of State University of Feira de Santana. In addition, the assays were entirely randomly mounted, four repetitions of 10 seeds for the vigor test and 50 seeds for the stress test. In the initial vigor, the seeds of all samples showed 100% of radicle emission at the first count. The seeds samples were affected by the addition of sodium chloride in the solution, exhibiting critical limit at 36dSm-1 for some samples. The radicular protrusion was affected with the decrease of the osmotic potential of the solution, which was caused by PEG 6000, limiting the germination in -0,9 MPa. The seed germination of E. velutina occurs at broad limits of temperature, from 5 to 40?C. Generally, the stress imposed by sodium chloride and PEG did not compromise the viability of the seed germinal power as the seeds of the samples showed high rate of germination when the ideal conditions were reestablished, excepting Cai?ara 2011. The great temperature of E. velutina germination occurs between 25 to 30?C. / A situa??o de estresse na caatinga tende a se agravar pelas mudan?as no clima onde se prev? maiores temperaturas, escassez de ?gua e maior saliniza??o do solo. Estudos que avaliem as diferen?as de toler?ncia de sementes aos estresses abi?ticos entre acessos ou gen?tipos da mesma esp?cie podem constituir ferramentas fisiol?gicas importantes e complementares para a sele??o de grupos de plantas mais adaptadas ?s press?es ambientais. Esse trabalho teve como objetivos comparar a vulnerabilidade de sementes de diferentes acessos de Erythrina velutina (Fabaceae) ? estresses abi?ticos; avaliar a qualidade fisiol?gica das sementes; e determinar os limites de toler?ncia aos estresses salino, h?drico e t?rmico. Os ensaios foram conduzidos nos laborat?rios de sementes da Embrapa Semi?rido (Petrolina-PE) e no de Germina??o da UEFS (Feira de Santana ?BA). Os ensaios foram montados em delineamento inteiramente casualizado, quatro repeti??es de 10 sementes para o teste de vigor e de 50 sementes para os estresses. Em rela??o ao vigor inicial as sementes de todos os acessos apresentaram 100% de emiss?o de rad?cula na primeira contagem. As sementes dos acessos foram afetadas pela adi??o de cloreto de s?dio na solu??o apresentando limite cr?tico de germina??o aos 36 dS.m-1 para alguns. A protrus?o radicular foi afetada tamb?m com a diminui??o do potencial osm?tico da solu??o causada pelo PEG 6000 tendo limite de germina??o em -0,9 MPa. A germina??o de sementes de E. velutina ocorreu sob amplo limites de temperatura, 5 a 40 ?C. De modo geral os estresses impostos pelo cloreto de s?dio e PEG n?o comprometeram a viabilidade do poder germinativo, visto que as sementes dos acessos apresentaram alta porcentagem de germina??o quando as condi??es ideais foram reestabelecidas, exceto as de Cai?ara 2011. A temperatura ?tima de germina??o de E. velutina ocorreu na faixa de 25 a 30 ?C.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-06-09T11:33:18Z No. of bitstreams: 1 470158 - Texto Completo.pdf: 900834 bytes, checksum: c9822e05947627069fb5b4fc9d1f6005 (MD5) / Made available in DSpace on 2015-06-09T11:33:18Z (GMT). No. of bitstreams: 1 470158 - Texto Completo.pdf: 900834 bytes, checksum: c9822e05947627069fb5b4fc9d1f6005 (MD5) Previous issue date: 2015-03-17 / The international market for essential oils has an annual turnover of 1.8 billion dollars, yet the Brazilian participation in this market is only 0.1%. The vast biodiversity found in Brazil, although still little explored in relation to the chemical composition of its flora, places the country in a very promising position in terms of increasing its future participation in the essential oils market. Basil (Ocimum basilicum L.) is a widely cultivated aromatic plant due to its medicinal properties. The importance of this species lies in the plant architecture, coloration and morphology of its leaves and flowers, in addition to the chemical composition of its essentials oils, giving specific aromas and having various uses in the culinary, perfume and pharmaceuticals industries. In this study, two experiments were performed with the aim of studying germination in simulated hypergravity and open-air cultivation, to evaluate the growth of the basil, its yield and essential oil composition. The extraction of the essential oils was carried out by steam distillation in a Clevenger apparatus. Quantitative and qualitative evaluation of the compounds was performed by gas chromatography coupled to a mass spectrometer detector (GC/MS). The mean values for germination under the effect of hypergravity simulation in protocols 1, 2 and 3 ranged from 81% to 87%. After this stage, the seeds exposed to simulated hypergravity were cultivated in the open-air and the fresh basil leaves were collected after 90 days to obtain the essential oils. The overall yield of oil was 0.2mL to 0.3mL and the essential oil was 0.3% to 0.4%. The largest and most predominant compound in this research was eugenol, ranging from 46% to 62%, with the second largest being linalool, which ranged from 22% to 28%. Further research in this area is essential to understand the real impact of simulated hypergravity on basil, and its effect on auxins and gene expression. / O mercado internacional de ?leos essenciais movimenta anualmente 1,8 bilh?es de d?lares e a participa??o brasileira nesse mercado ? de apenas 0,1%. A grande biodiversidade brasileira, ainda pouco explorada em rela??o ? composi??o qu?mica de sua flora, coloca o Brasil numa situa??o muito promissora para aumentar sua participa??o futura no mercado de ?leos essenciais. O manjeric?o (Ocimum basilicum L.) ? uma planta arom?tica largamente cultivada, devido ? suas propriedades medicinais. A import?ncia dessa esp?cie est? na arquitetura da planta, na colora??o e na morfologia das folhas e flores, al?m da composi??o qu?mica de seus ?leos essenciais, originando aromas espec?ficos e tendo utiliza??o variada na gastronomia, perfumaria e ind?stria farmac?utica. Nesse trabalho, foram realizados dois experimentos, com o objetivo de estudar a germina??o por hipergravidade simulada e o cultivo a c?u aberto para avaliar o crescimento do manjeric?o, rendimento e a composi??o do ?leo essencial. A extra??o do ?leo essencial foi feita por arraste a vapor em aparelho Clevenger. A avalia??o quantitativa e qualitativa dos compostos foi realizada por cromatografia gasosa acoplada a um detector de massas (CG/MS). As m?dias de germina??o deste trabalho foram de 81% a 87%. Ap?s essa etapa, as sementes sob efeito de hipergravidade simulada foram cultivadas a c?u aberto e, depois de 90 dias, foram coletadas folhas frescas de manjeric?o para obter ?leos essenciais. O rendimento total de ?leo foi de 0,2 mL a 0,3 mL e de ?leo essencial de 0,3%. a 0,4%. O composto majorit?rio e predominante em nossas pesquisas foi o eugenol, variando entre 46% a 62%, e o segundo maior composto foi o linalol, que ficou entre 22% a 28%. Pesquisas nessa ?rea s?o essenciais para entender o real impacto da hipergravidade simulada no manjeric?o, seu efeito na auxina e sua express?o g?nica.

Made available in DSpace on 2016-04-28T14:58:35Z (GMT). No. of bitstreams: 1 2008 - Thais Moreira Hidalgo de Almeida.pdf: 3286442 bytes, checksum: 491a402cc8bf5539730d0625fcbf7b31 (MD5) Previous issue date: 2008-02-28 / Germination, conservation and physical characteristics of seeds of four cacti species that occurs on Rio de Janeiro State coastal regions, Cereus fernambucensis Lem., Coleocephalocereus fluminensis (Miq.) Backeb., Pilosocereus arrabidae (Lem.) Byles & G.D. Rowley and Pilosocereus ulei (K. Schum.) Byles & G.D. Rowley were evaluated in this study. The first experiment, mounted in a completely randomized design and factorial system, tested two substrates (agar 1% and paper) and three temperatures (20?C, 25?C and 20-30?C) using four replicates of 40 seeds. The evaluation parameters were germination percentage (normal seedlings) and germination average time (ANOVA and Tukey; P<0,05). According to the germination analysis, the best combination of temperature and substrate is 20?C in agar for C. fernambucensis, 20?C and 20-30?C in paper or agar for C. fluminensis, 25?C in agar for P. arrabidae and 20, 25 and 20-30?C in paper, as well as, 20 and 25?C in agar for P. ulei. The use of agar 1% was suitable for germination of this cacti seeds, being necessary, however, studies that lead to reduction in the incidence of plant health problems. In another experiment the effects of storage and seed water content were evaluated on the germination percentage and germination average time of these four species. To better define the salts to be used in the seed drying process for this experiment, adsorption isotherms were built using static gravimetric method, with saturated salt solutions. The isotherms of all species showed a reverse sigmoidal pattern with increase of seed water content according to the increase in relative humidity. In the conservation experiment, also arranged in completely randomized design and factorial system, three different storage conditions (control, 30 days at 10?C and 30 days at -196?C) under three levels of water content, around 5, 7 and 9% (dry basis) were tested, with four replicates of forty seeds (ANOVA and Tukey; P<0,05) at the following conditions: C. fernambucensis in agar at 20?C, C. fluminensis and P. ulei in paper at 20?C and P. arrabidae in agar at 25?C. All four species showed dehydration and sub-zero temperature tolerance. Seed viability and four species showed dehydration and sub-zero temperature tolerance. Seed viability and vigour showed no changes when stored with water content around 7%, at 10?C and cryopreserved at -196?C. Cryopreservation can be recommended for the storage of this cacti species. / As caracter?sticas f?sicas, germina??o e conserva??o de sementes de quatro esp?cies de cact?ceas ocorrentes nas ?reas litor?neas do estado do Rio de Janeiro, Cereus fernambucensis Lem., Coleocephalocereus fluminensis (Miq.) Backeb., Pilosocereus arrabidae (Lem.) Byles & G.D. Rowley e Pilosocereus ulei (K. Schum.) Byles & G.D. Rowley foram avaliadas neste estudo. O primeiro experimento, montado em delineamento inteiramente casualizado e esquema fatorial, testou dois substratos (?gar 1% e sobre papel ) e tr?s temperaturas (20?C, 25?C e 20-30?C) utilizando quatro repeti??es de 40 sementes. Os par?metros de avalia??o foram porcentagem de germina??o (pl?ntulas normais) e tempo m?dio de germina??o (ANAVA e Tukey; P<0,05). De acordo com a an?lise de germina??o, as melhores temperaturas e substratos para a germina??o de sementes de C. fernambucensis s?o 20?C e ?gar; para C. fluminensis, 20?C e 20-30?C, tanto no substrato papel como em ?gar, para P. arrabidae, 25?C em ?gar e para P. ulei em papel a 20, 25 e 20-30?C e ?gar a 20 e 25?C. O uso de ?gar 1% se mostrou adequado a germina??o das sementes destas cact?ceas, sendo necess?rios, no entanto, estudos que levem a redu??o da incid?ncia de problemas fitossanit?rios. Em outro experimento foram avaliados os efeitos de armazenamento e teor de ?gua das sementes sobre o percentual e o tempo m?dio de germina??o destas quatro esp?cies. Para melhor defini??o dos sais a serem usados na secagem das sementes para este experimento, foram constru?das isotermas de adsor??o utilizando-se o m?todo gravim?trico, est?tico, com solu??es salinas saturadas. As isotermas de todas as esp?cies revelaram um padr?o sigmoidal inverso com o acr?scimo de teor de ?gua das sementes em fun??o do aumento da umidade relativa do ar. No experimento de conserva??o, tamb?m montado em delineamento inteiramente casualizado e esquema fatorial, foram testadas tr?s diferentes condi??es de armazenamento (controle, 30 dias a 10?C e 30 dias a -196?C) sob tr?s diferentes teores de ?gua, pr?ximos a 5, 7 e 9% (base seca), utilizando quatro repeti??es de 40 sementes (ANAVA e Tukey; P<0,05) e nas seguintes condi??es: C. fernambucensis em ?gar a 20?C, C. fluminensis e P. ulei em papel a 20?C e P. arrabidae em ?gar a 25?C. As quatro esp?cies demonstraram toler?ncia a desidrata??o e a temperatura sub-zero. A viabilidade e o vigor das sementes n?o apresentaram altera??es quando armazenados com teores de ?gua pr?ximos a 7%, tanto em c?mara fria a 10?C como criopreservado a -196?C. A criopreserva??o pode ser recomendada para o armazenamento das esp?cies estudadas.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-11-23T17:01:38Z No. of bitstreams: 1 2013 - L?lian Guimar?es de Almeida.pdf: 713686 bytes, checksum: 57c134f17845f669da63a2f32063c483 (MD5) / Made available in DSpace on 2018-11-23T17:01:38Z (GMT). No. of bitstreams: 1 2013 - L?lian Guimar?es de Almeida.pdf: 713686 bytes, checksum: 57c134f17845f669da63a2f32063c483 (MD5) Previous issue date: 2013-07-22 / CAPES / The objective of this study was to evaluate the imbibition and white lupine seeds physiological quality as affected by the pre-sowing treatments using immersion on the solutions with distinct micronutrients. The experimental design was conducted in a completely randomized in factorial (3 lots x 7 treatments) with four replications, by period of evaluation (start and after four months of storage). There were used three white lupine seed lots and seven distinct pre-sowing treatments (soaked or not soaked seeds on distilled water and in solution of boric acid (H3BO3), zinc sulphate (ZnSO4), manganese sulphate (MnSO4), copper sulphate (CuSO4), and molybdic acid (H2MoO4)). The seeds were evaluated to the imbibition and subsequently were submitted to the germination and vigor tests (first count of germination and electrical conductivity, dry weight and length of seedlings), as well the determinate of the seeds nutrients content. From the results it can be concluded that there was decreased speed of water absorption in the seed lot with lower physiological quality in the presence of manganese and molybdenum. Seed treatment by immersion in distilled water and in solutions of micronutrients favored physiological seed quality of three lots of white lupine. / O objetivo do trabalho foi avaliar a embebi??o e a qualidade fisiol?gica de sementes de tremo?o branco ap?s terem sido submetidas ao tratamento de pr?-semeadura por imers?o em solu??es de micronutrientes. O delineamento experimental adotado foi o inteiramente casualizado, em esquema fatorial (3 lotes x 7 tratamentos), com quatro repeti??es, por per?odo de avalia??o (inicial e ap?s quatro meses de armazenamento). Foram utilizados tr?s lotes de sementes de tremo?o branco e sete distintos tratamentos de pr?-semeadura (sementes imersas ou n?o em ?gua destilada e em solu??o contendo ?cido b?rico (H3BO3), sulfato de zinco (ZnSO4), sulfato de mangan?s (MnSO4), sulfato de cobre (CuSO4) e ?cido mol?bdico (H2MoO4)). As sementes foram avaliadas quanto ? embebi??o e, em seguida, submetidas aos testes de germina??o e de vigor (primeira contagem e condutividade el?trica, comprimento e massa de pl?ntulas) bem como ? avalia??o do teor de nutrientes. Pelos resultados pode-se concluir que houve diminui??o da velocidade de absor??o de ?gua nas sementes do lote com menor qualidade fisiol?gica inicial, na presen?a de mangan?s e molibd?nio. O tratamento de sementes via imers?o em ?gua destilada e em solu??es de micronutrientes favoreceu a qualidade fisiol?gica das sementes dos tr?s lotes de tremo?o branco.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T14:01:55Z No. of bitstreams: 5 7.pdf: 11015132 bytes, checksum: 0a2a0824ce976c86d274a0563f3f447c (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-10T13:11:09Z (GMT) No. of bitstreams: 5 7.pdf: 11015132 bytes, checksum: 0a2a0824ce976c86d274a0563f3f447c (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-10T13:11:09Z (GMT). No. of bitstreams: 5 7.pdf: 11015132 bytes, checksum: 0a2a0824ce976c86d274a0563f3f447c (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2012 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / O objetivo deste trabalho foi avaliar a influ?ncia de ambientes, substratos, prog?nies e proced?ncias na emerg?ncia e crescimento de pl?ntulas de pequi. O experimento foi conduzido nos ambientes casa de vegeta??o, casa de sombra e c?u aberto, onde foram avaliadas diferentes matrizes dentro dos substratos S1(terra de subsolo de textura m?dia), S2(70% de vermiculita e 30% de moinha de carv?o) e S3 (30% de moinha de carv?o, 50 % de vermiculita e 20% de areia). Os dados de germina??o foram analisados pelo teste de qui-quadrado para independ?ncia, enquanto as vari?veis de di?metro e altura de pl?ntulas pela an?lise de vari?ncia. Os resultados foram significativos (p<1%) para prog?nies, substratos e ambientes em rela??o ? taxa de emerg?ncia. O substrato S1 e o ambiente c?u aberto obtiveram os maiores percentuais de emerg?ncia de pl?ntulas (7,54% e 7,59%), j? o substrato S1 dentro da casa de sombra foi a intera??o que proporcionou o melhor resultado (12,61%). A emerg?ncia se correlacionou positivamente com a densidade e temperatura dos substratos e negativamente com sua capacidade de reten??o de ?gua, al?m de ser inversamente proporcional ? press?o de vapor d??gua do ambiente. O substrato S3 e a casa de vegeta??o contribu?ram isoladamente para as maiores m?dias de di?metro do coleto e altura de pl?ntulas, sendo sua intera??o significativamente superior ?s demais combina??es de substratos e ambientes para a vari?vel altura. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2012. / ABSTRACT The objective of this study was to evaluate the influence of environments, substrates, progenies and provenances in the emergence and seedling growth of pequi. The experiment was conducted in a greenhouse environment, the shade and open air, where they were evaluated in different matrices of the substrates S1 (subsoil texture average), S2 (70% vermiculite and 30% charcoal powder) and S3 (30% of charcoal powder, vermiculite 50% and 20% sand). The germination data were analyzed by chi-square test for independence, while the variables of diameter and height of seedlings by analysis of variance. The results were significant (p <1%) for progeny, substrates and environments in relation to the rate of emergence. The substrate S1 open and the environment had the highest percentage of seedling emergence (7.54% and 7.59%), since the substrate S1 within the shade was the interaction that yielded better results (12.61%) . The emergence of positively correlated to the density and temperature of the substrate and negatively with their ability to retain water, and is inversely proportional to the pressure of water vapor from the environment. The substrate S3 and greenhouse alone contributed to the higher average stem diameter and height of seedlings, and their interaction significantly superior to other combinations of substrates and environments for the variable height.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T13:30:34Z No. of bitstreams: 5 32.pdf: 421716 bytes, checksum: f1dbd1cd2f5a9514dcb522400e36103c (MD5) license_url: 49 bytes, checksum: fd26723f8d7edacdb29e3f03465c3b03 (MD5) license_text: 20904 bytes, checksum: e09bf99e64678e4285abf3ef3e05412d (MD5) license_rdf: 22564 bytes, checksum: ff373f1ad387898ef8ba4f8bc9e37073 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T18:28:44Z (GMT) No. of bitstreams: 5 32.pdf: 421716 bytes, checksum: f1dbd1cd2f5a9514dcb522400e36103c (MD5) license_url: 49 bytes, checksum: fd26723f8d7edacdb29e3f03465c3b03 (MD5) license_text: 20904 bytes, checksum: e09bf99e64678e4285abf3ef3e05412d (MD5) license_rdf: 22564 bytes, checksum: ff373f1ad387898ef8ba4f8bc9e37073 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-27T18:28:44Z (GMT). No. of bitstreams: 5 32.pdf: 421716 bytes, checksum: f1dbd1cd2f5a9514dcb522400e36103c (MD5) license_url: 49 bytes, checksum: fd26723f8d7edacdb29e3f03465c3b03 (MD5) license_text: 20904 bytes, checksum: e09bf99e64678e4285abf3ef3e05412d (MD5) license_rdf: 22564 bytes, checksum: ff373f1ad387898ef8ba4f8bc9e37073 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2008 / O assa-peixe (Vernonia polyanthes Less.) esp?cie pertencente ? fam?lia Asteraceae, possui propriedades medicinais e tem potencial ap?cola. Apesar de seu intenso uso popular, s?o escassas as informa??es sobre os fatores que condicionam a germina??o e o armazenamento de suas sementes. Dessa forma, o objetivo do presente trabalho foi avaliar a qualidade fisiol?gica de sementes de assa-peixe em rela??o ao efeito dos fatores temperatura/luz, em diferentes tipos de c?maras, e a influ?ncia dos diferentes tipos de ambientes/embalagens, durante o per?odo de armazenamento. A pesquisa foi dividida em duas partes. A primeira parte consistiu em avaliar o efeito dos fatores temperatura e luz sobre o comportamento fisiol?gico das sementes de assa-peixe, atrav?s dos testes de vigor (teste de primeira contagem da germina??o e ?ndice de velocidade de germina??o) e germina??o, em diferentes tipos de c?maras, sob temperaturas constantes (Mangelsdorff e B.O.D.) e alternadas (B.O.D.), na presen?a e aus?ncia de luz. Os tratamentos foram dispostos em arranjo fatorial, com delineamento inteiramente casualizado e os dados foram submetidos ? an?lise de vari?ncia, com as m?dias comparadas entre si pelo teste de Tukey a 5% de probabilidade. Pelos resultados constatou-se que esta esp?cie comportou-se como ?fotobl?stica preferencial?, pelo fato da germina??o, ter ocorrido na presen?a e na aus?ncia de luz, por?m, esta foi favorecida em presen?a de luz. As maiores porcentagens de germina??o de sementes de assa-peixe foram obtidas a 25?C, independente do tipo de c?mara utilizada (Mangelsdorff ou B.O.D.), e na faixa de temperatura alternada 15-25?C (em B.O.D.), na presen?a de luz. As sementes de assa-peixe n?o germinaram sob temperatura de 40?C. A segunda parte da pesquisa consistiu em avaliar a influ?ncia dos diferentes tipos de ambientes e embalagens e do tempo de armazenamento sobre a qualidade fisiol?gica das sementes. Foram testados tr?s per?odos de armazenamento (0, 136 e 230 dias), sobre duas condi??es, em geladeira (5 ? 2?C) e ambiente de laborat?rio (22 ? 2?C), e tr?s tipos de embalagens (vidro, pl?stica e multifoliada). Determinou-se a porcentagem da umidade, vigor (teste de primeira contagem da germina??o e ?ndice de velocidade de germina??o) e germina??o. Os tratamentos foram dispostos em arranjo fatorial, com delineamento inteiramente casualizado e os dados foram submetidos ? an?lise de vari?ncia, com as m?dias comparadas entre si pelo teste Tukey a 5% de probabilidade. Foi verificado que o armazenamento das sementes de assa-peixe, em geladeira, proporcionou maiores valores para vigor e germina??o, com a embalagem de vidro apresentando melhor desempenho, comparados aos obtidos em ambiente de laborat?rio, independente da embalagem utilizada. O vigor e a germina??o das sementes de assa-peixe decrescem em fun??o do tempo, durante os 230 dias de armazenamento. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2008. / ABSTRACT The Vernonia polyanthes Less. is species belonging to the family Asteraceae has medicinal properties and have the potential apiculture. Despite their intense use popular, there are few information about the factors that affect the germination and storing their seeds. Thus, the objective of this study was the physiological quality of seeds of Vernonia polyanthes Less. in relation to the effect of factors temperature/light, in different types of chambers, and the influence of different types of environments/packaging, during the period of storage. The survey was divided into two parts. The first part of the research was to evaluate the effect of the factors temperature and light on the behavior physiological seeds of V. polyanthes Less., through testing of vigor (test first count of germination and germination speed index) and germination, using different types of chambers, under constant temperatures (Mangelsdorff and B.O.D.) and alternate temperatures (B.O.D.), in the presence and absence of light. The experimental design was completely randomized, in factorial scheme and the averages compared were by Tukey test at 5%. The species, behaved itself as "preferential fotobl?stica" because of germination, have occurred in the presence and absence of light, but this was better in the presence of light. The higher germination percentages the seeds of V. polyanthes Less. were obtained at 25?C, regardless of the type of chamber used (Mangelsdorff and B.O.D.), and on diary alternating temperatures 15-25?C (in B.O.D.), in the presence of light. The temperature of 40?C was not germination of seeds of V. polyanthes Less.. The second part of the research was to evaluate the influence of different types of environments and packaging and time of storage of seeds of V. polyanthes on the physiological quality seeds. It was tested three periods of storage (0, 136 and 230 days), on two conditions, in refrigerator (5? 2 C) and laboratory environment (22 ? 2 C), and three types of packaging (glass, plastic and multilayer). It was determined the percentage moisture, vigor (test first count of germination and germination speed index) and germination. The experimental design was completely randomized, in factorial scheme and the averages compared were by Tukey test at 5%. It was found that the storage of seeds of V. polyanthes Less. in refrigerator had better values for vigor and germination, with a pack of glass showing better performance, that when stored in laboratory conditions, regardless of the type of packaging used. The vigor and germination the seeds decreased after 230 days of storage.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-01-14T21:02:15Z No. of bitstreams: 1 BrunoGomesDeNoronha_DISSERT.pdf: 1776336 bytes, checksum: 1f763accb352077181c730c9182cbba6 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-01-19T22:02:12Z (GMT) No. of bitstreams: 1 BrunoGomesDeNoronha_DISSERT.pdf: 1776336 bytes, checksum: 1f763accb352077181c730c9182cbba6 (MD5) / Made available in DSpace on 2016-01-19T22:02:12Z (GMT). No. of bitstreams: 1 BrunoGomesDeNoronha_DISSERT.pdf: 1776336 bytes, checksum: 1f763accb352077181c730c9182cbba6 (MD5) Previous issue date: 2014-12-16 / Os principais testes que avaliam a qualidade de sementes s?o destrutivos e exigem tempo, o que ? considerado longo e caro na cadeia que envolve a produ??o e comercializa??o das sementes. Deste modo, t?cnicas que viabilizem a redu??o do tempo que se gasta para se avaliar a qualidade de lotes de sementes ? muito favor?vel, do ponto de vista t?cnico, econ?mico e cient?fico. A an?lise de imagens de sementes tanto por meio de raio-X como por imagens digitais representam alternativas para estes setores, al?m de serem reproduz?veis e r?pidos, dando maior agilidade e autonomia ?s atividades dos sistemas de produ??o. O objetivo do trabalho foi analisar a morfologia interna de sementes desta esp?cie por meio de imagens radiografadas e a efici?ncia da determina??o do incremento de ?rea de sementes durante a embebi??o, por meio da an?lise de imagens e compar?-los com os resultados dos testes de germina??o e vigor na avalia??o da qualidade fisiol?gica das sementes. Para os testes de raio-X, as sementes foram expostas por 0,14 segundos ? radia??o de 40kV e 2,0 mAs. As imagens obtidas foram analisadas utilizando o programa ImageJ e postas a germinar posteriormente em c?mara do tipo B.O.D ? 27?C, no qual realizou-se a compara??o dos resultados obtidos nos testes de germina??o. Para a determina??o do teste de incremento de ?rea (I.A%), foram utilizadas sementes com e sem tegumento, mantidas em c?mara do tipo B.O.D a 15? e 20?C, as sementes foram fotografadas antes e ap?s o per?odo de embebi??o, os resultados foram comparados com os ?ndices de germina??o. Para o teste de raio-X, observouse que sementes com ?rea vazia superior a 20%, apresentaram maior percentual de pl?ntulas anormais. E a an?lise de incremento de ?rea, evidenciou que ? poss?vel ranquear os lotes ap?s 8 horas de embebi??o a 15?C de acordo com os testes de germina??o e vigor / Several tests that evaluate the quality of seeds are destructive and require time, which is considered long and expensive in the processes that involves the production and marketing of seed. Thus, techniques that allow reducing the time related to assess the quality of seed lots is very favorable, considering the technical, economic and scientific point of view. The techniques images of seed analyzed both by X-ray such as digital images, represent alternative for this sector, and are considered reproducible and fast, giving greater flexibility and autonomy to the activities of production systems. Summarily, the objective was to analyze the internal morphology of seeds of this species through x-rayed images and the efficiency of weed seed area increased during soaking through image analysis and compare them with the results of germination tests and force the evaluation of physiological seed quality. For X-ray tests, the seeds were exposed for 0.14 seconds at radiation 40kV and 2.0 mAs. Were analyzed images using the ImageJ program and subsequently put to germinate in B.O.D chamber at 27 ? C, in which there was the comparison of results for germination. To determine the test area increase (% IA), seeds were used with and without seed coat, maintained the B.O.D chamber at 15 ? to 20 ? C, the seeds were photographed before and after the soaking period, the results were compared to the germination rates. For the X-ray test, it was observed that seeds with empty area greater than 20%, showed a higher percentage of abnormal seedlings. And the area increment analysis showed that it is possible to rank the batch after 8 hours of imbibition at 15 ? C according to the germination and vigor tests