Abstract Background: Gastroenteritis is a common infection and the leading cause of morbidity worldwide and is mostly caused by viruses. Outbreaks appear in both developed and developing countries and result in large economic costs. Rapid detection is important for appropriate treatment, control and to prevent the spread of infection.  Objective: Evaluation and performance comparison between the BioFire®FilmArray® Torch System gastrointestinal panel and the Molecular BD MAXTMenteric viral panel to indicate a multiplex method for viral gastroenteritis diagnostic in a routine laboratory setting.  Material and methods: In this study, 58 different samples were used which consisted of selected stool specimens from patients who were tested and treated for gastroenteritis infection at Uppsala Academic Hospital and Norrlands University Hospital in Umeå during 2018-2021, samples from Quality control for molecular diagnostics viral gastroenteritis EQA pilot study during 2018-2019 and cultivated strains of different adenovirus species from 2018. All samples were analyzed with both systems for comparison of detected pathogens.  Results: Sensitivity and specificity values were 95% and 100% respectively for the BioFire®FilmArray®Torch System and 100% and 93.3% for the BD MAXTMSystem.   Conclusions: Bothsystems are rapid and adequate diagnostic tools. The BioFire®FilmArray®Torch System with greater coverage has the ability of detecting more pathogens and is more promising particularly in the occasional infection circumstance. The BD MAXTMSystem demonstrated almost the same results and seems to be a better option in times of an outbreak when the numbers of patients are significantly higher.

The relationship between narrative and game mechanics has been researched under terms such as ludonarrative dissonance, ludonarrative resonance and narrative mechanics. Yu-Gi-Oh! is a Trading Card Game which contains game mechanics that symbolically represents sections of the Arthurian legend. In this thesis the mechanics of specific Yu-Gi-Oh! cards were compared to the Arthurian legend. From this analysis were design guidelines derived, on how to represent a narrative with game mechanics. Game objects can be referenced by listing associated cards. The name of a mechanic can help in symbolically tell a narrative. The exclusion of a mechanic can also represent something. The conditions for a mechanic to be available is an area where narrative can be implemented. If a narrative mechanic is surrounded by many other mechanics, the symbolism can be “drowned out”. There is a balance between effective symbolism and a good game mechanic.

Die Umweltfernerkundung hat in den vergangenen zwei Jahrzehnten enorme Fortschritte erzielt und bietet Potenzial zur Erweiterung behördlicher Geodatenbestände und vielfältiger Anwendung. Insbesondere abbildende Hyperspektraldaten und Thermaldaten bieten die Möglichkeit für die Bereitstellung neuer thematische Informationen (GIS-Layer). Ein zentrales Einsatzgebiet ist die Analyse, Visualisierung und Bereitstellung von Informationen zu den Wirkungsketten grüner und blauer Infrastruktur in Landschaften und Siedlungen. Der Beitrag vermittelt einen Überblick zu Anwendungen, Aspekten und Anforderungen an die Akquise von Hyperspektraldaten und Thermaldaten.

The concept of glycemic index (GI) describes postprandial blood glucose concentration. Diets with a low GI can reduce the risk of coronary heart disease, type II diabetes and certain cancers. Bread is the main source of carbohydrates in the Swedish traditional diet, and is classified as a food with a medium to high GI. Previous studies on the subject resulted in lower GI value when adding bean extract and sourdough. The aim of the study was to develop a low GI wheat sourdough bread containing white bean flour, and to investigate the effects of different preparations, soaked, soaked and boiled, soaked and microwaved, of white bean and bread making on the content of resistant starch (RS). Three bread prototypes, containing different amounts of beans that had been soaked for 14 hours and microwaved for 6 minutes, plus a control bread were prepared in a food laboratory. RS was quantified in ingredients, dough and bread, and the in vitro GI ​​was determined for all breads. The GI for all bread prototypes was > 70, and was categorized as high GI, but the in vitro glycemic load (GL) was categorized as medium (11-16). The quantification of RS content was affected by the activity of the a-amylase inhibitor found in raw white beans. Without heat preparation, the a-amylase inhibitor is active, which results in a falsely high result for RS. The process of producing a bread with a low GI using white bean flour did not succeed in this study, even though a high RS content should result in low GI. The development of low GI sourdough bread with white bean flour requires extended studies. / Begreppet glykemiskt index (GI) beskriver postprandial blodsockerkoncentration. Dieter med lågt GI kan minska risken för kranskärlssjukdom, diabetes typ II och vissa cancerformer. Bröd är den främsta källan till kolhydrater i den svenska traditionella kosten, och klassas som ett livsmedel med medelhögt till högt GI. Tidigare studier inom ämnet har visat att vid tillsats av bönextrakt och surdeg till bröd, erhålls ett lägre GI-värde. Syftet med studien var att utveckla ett vetesurdegsbröd med lågt GI innehållande mjöl från vita bönor, samt att undersöka hur innehållet av resistent stärkelse (RS) påverkas av olika beredningar av vita bönor, blötläggning, blötläggning och kokning, blötläggning och mikrovågsvärmning, samt brödtillverkning. Tre brödprototyper, innehållande olika mängd av mjöl från vita bönor som har blötlagts i 14 timmar och mikrovågsvärmda i 6 minuter, plus ett kontrollbröd framställdes i ett livsmedelslaboratorium. RS kvantifierades i ingredienser, deg och bröd, och in vitro GI bestämdes för alla bröd. GI för alla brödprototyper var > 70, och kategoriserades som högt GI, men den in vitro glykemiska belastningen (GL) kategoriserades som medium (11-16). Kvantifieringen av RS-innehållet påverkades av aktiviteten hos a-amylasinhibitorn som finns i råa vita bönor. Utan värmeberedning är a-amylashämmaren aktiv, vilket ger ett felaktigt högt resultat för RS. Processen att framställa ett bröd med lågt GI med mjöl från vita bönor lyckades inte i denna studie, även om en hög RS-halt borde resultera i lågt GI. Det krävs fler utökade studier för att framställa ett surdegsbröd med lågt GI, innehållande mjöl från vita bönor.

京都大学 / 新制・課程博士 / 博士(医学) / 甲第24884号 / 医博第5018号 / 新制||医||1068(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 鈴木 実, 教授 小濱 和貴, 教授 中島 貴子 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM

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I contend that young students should be introduced to intercultural contemporary music, as this exposure brings benefits to their artistic development and fosters appreciation of other cultures. Variations sur un thème populaire coréen by Sung-Ki Kim (b. 1954) and Six Pieces for Piano ‘Nori' by Chung-Sock Kim (b. 1940) are effective pedagogical works that fit perfectly into the intercultural mold mentioned above, and both are suitable for late intermediate or early advanced level students. A detailed comparison of these two works can help instructors understand the ways by which these composers incorporate Korean folk materials and blend them with Western contemporary techniques. An analysis of Sung-Ki Kim's Variations sur un theme populaire coréen and Chung-Sock Kim's Six Pieces for Piano ‘Nori' can be divided into three categories: harmony, rhythm, and performance-related aspects. By analyzing these two pieces, these study illustrates in greater depth their intercultural aspects, showing the way by which both composers merged traditional Korean folk idioms through the inclusion of traditional Korean rhythms, and the imitation of sounds of several traditional Korean instruments with Western contemporary technique such as non-traditional sounds and use of sostenuto pedal. Finally, this study provides some practicing suggestions and listing exercise on how to practice musical and technical challenges.

Nous avons précédemment montré que les cellules musculaires lisses vasculaires(CMLV) des rats spontanément hypertendus (SHR) présentent une expression augmentée des protéines G inhibitrices (Gi) et une prolifération cellulaire accrue par rapport aux CMLV des rats Wystar-Kyoto (WKY). Le niveau d'AMPc s’est également avéré plus faible dans les CMLV de SHR. La présente étude a donc été entreprise afin d'examiner la contribution de la diminution du niveau intracellulaire d'AMPc à l’augmentation de l'expression des protéines Gi et à la prolifération accrue des CMLV de SHR et de continuer à explorer les mécanismes moléculaires sous-jacents responsables de cette réponse. Les CMLV de SHR ont montré par rapport aux CMLV des WKY une expression accrue de Giα-2 et Giα-3 qui a été diminué d'une manière dépendante de concentration par le dbcAMP, un analogue d'AMPc perméable à la membrane cellulaire. En outre, les fonctions augmentées des protéines Gi comme démontrées par l'amplification de l’inhibition de l'adénylate cyclase par les hormones inhibitrices et l'activité forskoline (FSK)-stimulée de l’adénylate cyclase par une faible concentration de GTPγS dans les CMLV de SHR ont également été restaurées aux niveaux de WKY par le dbcAMP. La prolifération accrue des CMLV de SHR a également été atténuée par le dbcAMP et la forskoline, un activateur de l'adénylate cyclase. De plus, dbcAMP a restauré la production augmentée d'anion superoxyde (O2-), l'activité de la NAD(P)H oxydase et l’expression accrue des protéines Nox 4 et p47phox observée dans les CMLV de SHR jusqu’au niveau contrôle. Par ailleurs, la phosphorylation accrue des PDGF-R, EGF-R, c-Src et ERK1/2 énoncée par les CMLV de SHR a également été diminuée par le dbcAMP d'une manière dépendante de concentration. Ces résultats suggèrent que le niveau réduit d'AMPc intracellulaire montré par les CMLV de SHR contribue à l'expression accrue des protéines Gi et à l’hyperprolifération cellulaire à travers l’augmentation du stress oxydatif, la transactivation des EGF-R, PDGF-R et la voie de signalisation des MAP kinases. / We have previously shown that vascular smooth muscle cells (VSMC) from spontaneously hypertensive rats (SHR) exhibit enhanced expression of inhibitory G proteins (Gi) and enhanced cell proliferation as compared to VSMC from Wystar-Kyoto rats (WKY). The levels of cAMP were shown to be decreased in VSMC from SHR. The present study was therefore undertaken to examine the contribution of the decreased intracellular level of cAMP in the enhanced expression of Gi proteins and increased proliferation of VSMC from SHR and to further explore the underlying molecular mechanisms responsible for this response. VSMC from SHR showed an enhanced expression of Giα-2 and Giα-3 as compared to VSMC from WKY which was decreased in a dose-dependent manner by dbcAMP, a cell-permeable cAMP analog. In addition, the enhanced functions of Gi proteins as demonstrated by enhanced inhibition of adenylyl cyclase by inhibitory hormones and forskolin (FSK)-stimulated adenylyl cyclase activity by low concentration of GTPγS in VSMC from SHR were also restored to the WKY levels by dbcAMP. The enhanced proliferation of VSMC exhibited by SHR was also attenuated by dbcAMP and forskolin, an activator of adenylyl cyclase. In addition, dbcAMP also restored the increased production of superoxide anion (O2-), NAD(P)H oxidase activity and enhanced expression of Nox 4 and p47phox proteins observed in VSMC from SHR to control levels. Furthermore, the increased phosphorylation of PDGF-R, EGF-R, c-Src and ERK1/2 exhibited by VSMC from SHR were also decreased by dbcAMP in a dose-dependent manner. These results suggest that decreased levels of intracellular cAMP exhibited by VSMC from SHR contributes to the enhanced expression of Gi proteins and hyperproliferation through increasing oxidative stress and transactivation of EGF-R, PDGF-R and MAP kinase signaling pathway.

Nous avons précédemment montré que les cellules musculaires lisses vasculaires(CMLV) des rats spontanément hypertendus (SHR) présentent une expression augmentée des protéines G inhibitrices (Gi) et une prolifération cellulaire accrue par rapport aux CMLV des rats Wystar-Kyoto (WKY). Le niveau d'AMPc s’est également avéré plus faible dans les CMLV de SHR. La présente étude a donc été entreprise afin d'examiner la contribution de la diminution du niveau intracellulaire d'AMPc à l’augmentation de l'expression des protéines Gi et à la prolifération accrue des CMLV de SHR et de continuer à explorer les mécanismes moléculaires sous-jacents responsables de cette réponse. Les CMLV de SHR ont montré par rapport aux CMLV des WKY une expression accrue de Giα-2 et Giα-3 qui a été diminué d'une manière dépendante de concentration par le dbcAMP, un analogue d'AMPc perméable à la membrane cellulaire. En outre, les fonctions augmentées des protéines Gi comme démontrées par l'amplification de l’inhibition de l'adénylate cyclase par les hormones inhibitrices et l'activité forskoline (FSK)-stimulée de l’adénylate cyclase par une faible concentration de GTPγS dans les CMLV de SHR ont également été restaurées aux niveaux de WKY par le dbcAMP. La prolifération accrue des CMLV de SHR a également été atténuée par le dbcAMP et la forskoline, un activateur de l'adénylate cyclase. De plus, dbcAMP a restauré la production augmentée d'anion superoxyde (O2-), l'activité de la NAD(P)H oxydase et l’expression accrue des protéines Nox 4 et p47phox observée dans les CMLV de SHR jusqu’au niveau contrôle. Par ailleurs, la phosphorylation accrue des PDGF-R, EGF-R, c-Src et ERK1/2 énoncée par les CMLV de SHR a également été diminuée par le dbcAMP d'une manière dépendante de concentration. Ces résultats suggèrent que le niveau réduit d'AMPc intracellulaire montré par les CMLV de SHR contribue à l'expression accrue des protéines Gi et à l’hyperprolifération cellulaire à travers l’augmentation du stress oxydatif, la transactivation des EGF-R, PDGF-R et la voie de signalisation des MAP kinases. / We have previously shown that vascular smooth muscle cells (VSMC) from spontaneously hypertensive rats (SHR) exhibit enhanced expression of inhibitory G proteins (Gi) and enhanced cell proliferation as compared to VSMC from Wystar-Kyoto rats (WKY). The levels of cAMP were shown to be decreased in VSMC from SHR. The present study was therefore undertaken to examine the contribution of the decreased intracellular level of cAMP in the enhanced expression of Gi proteins and increased proliferation of VSMC from SHR and to further explore the underlying molecular mechanisms responsible for this response. VSMC from SHR showed an enhanced expression of Giα-2 and Giα-3 as compared to VSMC from WKY which was decreased in a dose-dependent manner by dbcAMP, a cell-permeable cAMP analog. In addition, the enhanced functions of Gi proteins as demonstrated by enhanced inhibition of adenylyl cyclase by inhibitory hormones and forskolin (FSK)-stimulated adenylyl cyclase activity by low concentration of GTPγS in VSMC from SHR were also restored to the WKY levels by dbcAMP. The enhanced proliferation of VSMC exhibited by SHR was also attenuated by dbcAMP and forskolin, an activator of adenylyl cyclase. In addition, dbcAMP also restored the increased production of superoxide anion (O2-), NAD(P)H oxidase activity and enhanced expression of Nox 4 and p47phox proteins observed in VSMC from SHR to control levels. Furthermore, the increased phosphorylation of PDGF-R, EGF-R, c-Src and ERK1/2 exhibited by VSMC from SHR were also decreased by dbcAMP in a dose-dependent manner. These results suggest that decreased levels of intracellular cAMP exhibited by VSMC from SHR contributes to the enhanced expression of Gi proteins and hyperproliferation through increasing oxidative stress and transactivation of EGF-R, PDGF-R and MAP kinase signaling pathway.

Dizertační práce je zaměřena na kompletní metodiku návrhu tří a čtyř prvkových flíčkových anténních řad, které neobsahují žádné filtrující části a přesto se chovají jako filtrující antény (filtény). Návrhová metodika kombinuje přístup pro návrh filtrů s přístupem pro anténní řady a zahrnuje tvarování frekvenčních odezev činitele odrazu a normovaného realizovaného zisku. Směr hlavního laloku přes pracovní pásmo je kontrolován také. S cílem kontrolovat tvary uvedených charakteristik, nové gi koeficienty jsou představeny pro návrh filtrujících anténních řad. Návrhová metodika byla ověřena na tří a čtyř prvkové filtrující anténní řadě přes frekvenční pásmo od 4,8 GHz do 6,8 GHz, pro šířku pásma celé struktury od 7 % do 14 % a pro požadovanou úroveň činitele odrazu od –10 dB do –20 dB. Celá metodika byla podpořena výrobou a měřením šesti testovacích vzorků filtrujících anténních řad s rozdílnými konfiguracemi. Ve všech případech se simulované a naměřené výsledky dobře shodují.