Made available in DSpace on 2014-06-11T19:22:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-04-05Bitstream added on 2014-06-13T19:49:05Z : No. of bitstreams: 1 dalia_ra_me_rcla.pdf: 910850 bytes, checksum: eac946a1f4330146a0755088ee83927b (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Apesar dos avanços nas pesquisas na área de atividade física e medicina, ainda existem diversas contradições a respeito do real risco e benefício da utilização do exercício físico resistido, principalmente o de força de alta intensidade, para hipertensos no que tange suas variáveis metabólicas. Desta forma, o objetivo desta dissertação foi analisar a influência de um programa de treinamento físico de alta intensidade sobre o metabolismo glicídico, protéico, lipídico e hormonal de ratos espontaneamente hipertensos. Para isso utilizamos ratos machos da linhagem de animais espontaneamente hipertensos (SHR) e ratos Wistar Kyoto (WKY), com 20 semanas de vida. Os animais foram divididos em quatro grupos: SHR Sedentário (SHRS); SHR Treinado (SHRT); WKY Sedentário (WKYS); WKY Treinado (WKYT). O treinamento físico de alta intensidade constituía de natação em tanques individuais, na qual os animais realizavam saltos através de uma mochila com velcro acoplada ao tórax do animal, na qual continha uma resistência equivalente a 50% da massa corporal do mesmo, na forma de chumbinhos. Eram realizadas quatro séries de 10 saltos, tendo um intervalo de 1 minuto entre cada série, em cinco dias na semana, durante um período de oito semanas consecutivas. Após o final do treinamento físico os animais passaram pelos testes de tolerância oral a glicose e tolerância a insulina, e após este período os animais forma eutanasiados e coletado soro, através do sangue coletado diretamente do coração, apara as dosagens de glicose, triglicerídeos, colesterol total, HLD e LDL colesterol, proteínas totais, albumina e creatina quinase (CK) e CK-MB, insulina e IGF-1, GH e ácidos graxos livres (A.G.L). Amostras do músculo gastrocnêmio, porções brancas e vermelhas, sóleo, fígado e coração foram coletados e utilizados para avaliação do conteúdo de glicogênio, proteína e DNA... / In spite of progress in researches in exercise and medicine area, still several contradictions exist regarding the real risk and benefit of use resistance exercise, mainly the high-intensity, for hypertension in what plays their metabolic variables. This way, objective of this dissertation was to analyze the influence of a high-intensity training program on the glucose, protein, lipids and hormonal metabolism of spontaneously hypertensive rats. For that we used male of lineage of spontaneously hypertensive rats (SHR) and Wistar Kyoto rats (WKY), with 20 weeks of life. The animals were divided in four groups: Sedentary SHR (SHRS); Trained SHR (SHRT); Sedentary WKY (WKYS); Trained WKY (WKYT). The high intensity training constituted of jumps in the water, in individual tanks, wearing a jacket coupled to the thorax containing a lead overload equivalent to 50% of the body mass. Four series of 10 jumps were accomplished, with an interval of 1 minute among each series, five days a week, during eight consecutive weeks. After the end of exercise training the animals went by the glucose tolerance tests and insulin tolerance test, and after this period all animals were killed and serum collected, through the blood collected directly of heart, it trims the glucose, triglycerides, total cholesterol, HLD and LDL cholesterol, total proteins, albumin and creatine kinase (CK) and CK-MB, insulin and IGF-1, GH and free fat acids (FFA). Samples of muscle gastrocnemius, white and red portions, soleus, liver and heart were collected and used for glycogen content, protein and DNA. In the hepatic and heart samples were still collected for analysis of IGF-1 and triglycerides. The supra-renal glands were removed for evaluation levels of total cholesterol through method enzymatic colorimeter. In the soleus muscle they were removed sliced for analyses synthesis and degradation for muscle... (Complete abstract click electronic access below)

Resumo: Apesar dos avanços nas pesquisas na área de atividade física e medicina, ainda existem diversas contradições a respeito do real risco e benefício da utilização do exercício físico resistido, principalmente o de força de alta intensidade, para hipertensos no que tange suas variáveis metabólicas. Desta forma, o objetivo desta dissertação foi analisar a influência de um programa de treinamento físico de alta intensidade sobre o metabolismo glicídico, protéico, lipídico e hormonal de ratos espontaneamente hipertensos. Para isso utilizamos ratos machos da linhagem de animais espontaneamente hipertensos (SHR) e ratos Wistar Kyoto (WKY), com 20 semanas de vida. Os animais foram divididos em quatro grupos: SHR Sedentário (SHRS); SHR Treinado (SHRT); WKY Sedentário (WKYS); WKY Treinado (WKYT). O treinamento físico de alta intensidade constituía de natação em tanques individuais, na qual os animais realizavam saltos através de uma mochila com velcro acoplada ao tórax do animal, na qual continha uma resistência equivalente a 50% da massa corporal do mesmo, na forma de chumbinhos. Eram realizadas quatro séries de 10 saltos, tendo um intervalo de 1 minuto entre cada série, em cinco dias na semana, durante um período de oito semanas consecutivas. Após o final do treinamento físico os animais passaram pelos testes de tolerância oral a glicose e tolerância a insulina, e após este período os animais forma eutanasiados e coletado soro, através do sangue coletado diretamente do coração, apara as dosagens de glicose, triglicerídeos, colesterol total, HLD e LDL colesterol, proteínas totais, albumina e creatina quinase (CK) e CK-MB, insulina e IGF-1, GH e ácidos graxos livres (A.G.L). Amostras do músculo gastrocnêmio, porções brancas e vermelhas, sóleo, fígado e coração foram coletados e utilizados para avaliação do conteúdo de glicogênio, proteína e DNA... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: In spite of progress in researches in exercise and medicine area, still several contradictions exist regarding the real risk and benefit of use resistance exercise, mainly the high-intensity, for hypertension in what plays their metabolic variables. This way, objective of this dissertation was to analyze the influence of a high-intensity training program on the glucose, protein, lipids and hormonal metabolism of spontaneously hypertensive rats. For that we used male of lineage of spontaneously hypertensive rats (SHR) and Wistar Kyoto rats (WKY), with 20 weeks of life. The animals were divided in four groups: Sedentary SHR (SHRS); Trained SHR (SHRT); Sedentary WKY (WKYS); Trained WKY (WKYT). The high intensity training constituted of jumps in the water, in individual tanks, wearing a jacket coupled to the thorax containing a lead overload equivalent to 50% of the body mass. Four series of 10 jumps were accomplished, with an interval of 1 minute among each series, five days a week, during eight consecutive weeks. After the end of exercise training the animals went by the glucose tolerance tests and insulin tolerance test, and after this period all animals were killed and serum collected, through the blood collected directly of heart, it trims the glucose, triglycerides, total cholesterol, HLD and LDL cholesterol, total proteins, albumin and creatine kinase (CK) and CK-MB, insulin and IGF-1, GH and free fat acids (FFA). Samples of muscle gastrocnemius, white and red portions, soleus, liver and heart were collected and used for glycogen content, protein and DNA. In the hepatic and heart samples were still collected for analysis of IGF-1 and triglycerides. The supra-renal glands were removed for evaluation levels of total cholesterol through method enzymatic colorimeter. In the soleus muscle they were removed sliced for analyses synthesis and degradation for muscle... (Complete abstract click electronic access below) / Orientador: Eliete Luciano / Coorientador: Maria Alice Rostom de Mello / Banca: Cláudio Alexandre Gobatto / Banca: Ricardo José Gomes / Mestre

京都大学 / 新制・課程博士 / 博士(医学) / 甲第23467号 / 医博第4774号 / 新制||医||1053(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 森信 暁雄, 教授 上野 英樹, 教授 小柳 義夫 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM

Bakgrund: The High Intensity Functional Exercise (HIFE) program har visats ha goda effekter på fysisk funktionsförmåga och ADL-förmåga för äldre personer boende på äldreboende och hjälpberoende i ADL. Det finns dock begränsat med kunskap om fysioterapeuters upplevelser och erfarenheter av att använda sig av HIFE på äldreboende. Denna studie kan bidra till förståelse för hur fysioterapeuter kan utföra högkvalitativ träning för denna population.   Syfte: Att undersöka fysioterapeuters upplevelser och erfarenheter av att använda sig av HIFE för äldre personer boende på äldreboende.    Metod: För att besvara syftet valdes en kvalitativ design och fem fysioterapeuter som har arbetat enligt HIFE på äldreboende i Sverige intervjuades genom enskilda semistrukturerade intervjuer. Intervjuerna analyserades med kvalitativ innehållsanalys.   Resultat: Utifrån insamlade data identifierades fyra kategorier med sammanlagt 17 underkategorier. Kategorierna var: Framgångsrik implementering kräver mångas engagemang, Viktigt att göra ett aktivt val av deltagare, Utmana deltagarna nära sina gränser och Effekter av träningen på många plan.   Konklusion: Samtliga fysioterapeuter gav uttryck för att det finns ett stort behov av HIFE på äldreboenden. HIFE ansågs lämpligt för många boende och möjliggör att utmana deltagarna till en hög intensitet så att de kan få en god effekt av träningen. För att lyckas krävs mångas engagemang innan, under och efter träningsperioden. / Background: The High Intensity Functional Exercise (HIFE) program has been shown to have good effect on physical function and ADL in elderly people living in nursing homes and dependent on assistance with activities in daily living (ADL). However, there is limited knowledge regarding physiotherapists' experiences of using HIFE in nursing homes. This study can help to understand how physiotherapists can perform high-quality training with this population.    Aim: To investigate physiotherapists' experiences of HIFE with elderly people living in nursing homes.    Methods: To achieve the objective of this study a qualitative design was chosen and five physiotherapists that have worked according to HIFE in nursing homes in Sweden were interviewed using a semi-structured approach. The interviews were analyzed using qualitative content analysis.    Results: Based on the collated data, four categories were identified with a total of 17 subcategories: Successful implementation requires the commitment of many, Important to actively choose participants, Push the participants close to their limits and Effects of the training seen on many levels.   Conclusion: All physiotherapists expressed a great need for HIFE in nursing homes. HIFE was considered suitable for many of the residents and made it possible to challenge the participants to a high intensity and thus saw a greater effect. In order to succeed, commitment is required from a number of people before, during and after the training period.

Endurance can be defined as the ability to maintain or to repeat a given force or power output. The sport performance-endurance relationship is a multi-factorial concept. However, evidence indicates that maximum strength is a major component. Conceptually, endurance is a continuum. The literature indicates that (a) maximum strength is moderately to strongly related to endurance capabilities and associated factors, a relationship that is likely stronger for high intensity exercise endurance (HIEE) activities than for low intensity exercise endurance (LIEE); (b) strength training can increase both HIEE and LIEE, the effect being greater for HIEE; (c) the volume of strength training plays a role in endurance adaptation; and (d) mechanical specificity and training program variables also play a role in the degree of adaptation.

Endurance can be defined as the ability to maintain or to repeat a given force or power output. The sport performance-endurance relationship is a multi-factorial concept. However, evidence indicates that maximum strength is a major component. Conceptually, endurance is a continuum. The literature indicates that (a) maximum strength is moderately to strongly related to endurance capabilities and associated factors, a relationship that is likely stronger for high intensity exercise endurance (HIEE) activities than for low intensity exercise endurance (LIEE); (b) strength training can increase both HIEE and LIEE, the effect being greater for HIEE; (c) the volume of strength training plays a role in endurance adaptation; and (d) mechanical specificity and training program variables also play a role in the degree of adaptation..

Embora os cavalos apresentem características excepcionais frente a situações de estresse na natureza, foi considerada a possibilidade do cavalo de corrida ser submetido, por vezes, a alguma situação de estresse, que afetasse sua saúde e bem estar. Foram verificadas alterações do número e da função de populações celulares implicadas com a defesa, principalmente neutrófilos e linfócitos. Trinta cavalos de corrida da raça puro-sangue inglês, locados no Jockey Club de São Paulo, na cidade de São Paulo, foram utilizados. Os animais foram divididos em dois grupos e as amostras de sangue foram coletadas em momentos diferentes da rotina dos animais. No grupo I, foram coletadas amostras onze dias antes da corrida (-11d), imediatamente após a corrida (0d), um dia após a corrida (+1d), dois dias após a corrida (+2d) e três dias após a corrida (+3d). Enquanto, no grupo II as amostras foram coletadas oito dias antes da corrida (-8d), imediatamente após a corrida (0d), um dia após a corrida (+1d) e cinco dias após a corrida (+5d). Foram realizados ensaios de fagocitose e burst oxidativo dos neutrófilos, determinação de cortisol sérico, ensaios de apoptose, linfoproliferação e hemograma. A função de fagocitose, tanto em porcentagem, quanto em intensidade, mostrou-se diminuída nas amostras coletadas imediatamente após a corrida, nos dois grupos de animais. Os valores médios de burst oxidativo das amostras coletadas imediatamente após a corrida, dos animais do grupo I apresentaram-se diminuídos. Não foram encontradas diferenças estatísticas significantes entre os resultados obtidos em relação às células apoptóticas, quando os diferentes momentos foram comparados; o mesmo ocorreu quando foi realizado o confronto dos valores do ensaio de linfoproliferação. Os valores médios dos níveis séricos de cortisol apresentaram-se aumentados no momento imediatamente após a corrida, em relação aos valores dos outros momentos, nos dois grupos de animais. Quanto aos dados de hemograma, houve aumento do número percentual de neutrófilos no momento imediatamente após a corrida quando foi realizada comparação com os demais momentos. Nos cavalos estudados, ainda que tenha sido encontrada uma diminuição transitória na função dos neutrófilos, a função adaptativa não chegou a ser acometida, que sugere que, embora se constitua em um exercício de alta intensidade, a fugacidade da corrida acabe por evitar danos maiores, em animais bem condicionados e adaptados a situações de estresse / Although horses can present exceptional responses during stress situations in nature, it was raised the possibility that racehorses under certain stress conditions could develop changes that may affect their health and welfare. Alterations of the number and function of cells that are involved with the defense response, mainly neutrophils and lymphocytes populations were verified. Thirty Thoroughbred racehorses housed at the Jockey Club de São Paulo, in São Paulo, Brazil, were used in the present study. The animals were divided in two groups and blood samples were collected at different moments of their routine. In group I, the samples were collected eleven days before race (-11d), immediately after race (0d), one day (+1d), two days (+2d) and three days after race (+3d). In group II, the samples were collected eight days before race (-8d), immediately after race (0d), one day (+1d) and five days after race (+5d). Phagocytosis assay and oxidative burst activity of neutrophils, serum cortisol determination, apoptosis and lymphocyte proliferation assay and haematology tests were performed. The percentage and intensity of phagocytosis functions decreased in samples collected immediately after race, in both groups of animals. The mean values of oxidative burst activity of samples collected immediately after race, from animals of group I also decreased. Statistically significant differences between the results were obtained in relation to the apoptotic cells, when compared on different moments and the same occurred in Relation to the lymphocyte proliferation values. The mean values of serum cortisol levels increased at the moment immediately after race when compared with the values of the other moments, in both groups of animals. In relation to the haematologycal values, the percentage of neutrophils was increased at the moment immediately after race when compared with the other moments. Even though a transitory reduction in the neutrophils function has occurred, the adaptive function was not impaired, which suggest that, in spite of the high intensity of this exercise, the short-term race can prevent further damages in well-conditioned and stress-adapted animals

Embora os cavalos apresentem características excepcionais frente a situações de estresse na natureza, foi considerada a possibilidade do cavalo de corrida ser submetido, por vezes, a alguma situação de estresse, que afetasse sua saúde e bem estar. Foram verificadas alterações do número e da função de populações celulares implicadas com a defesa, principalmente neutrófilos e linfócitos. Trinta cavalos de corrida da raça puro-sangue inglês, locados no Jockey Club de São Paulo, na cidade de São Paulo, foram utilizados. Os animais foram divididos em dois grupos e as amostras de sangue foram coletadas em momentos diferentes da rotina dos animais. No grupo I, foram coletadas amostras onze dias antes da corrida (-11d), imediatamente após a corrida (0d), um dia após a corrida (+1d), dois dias após a corrida (+2d) e três dias após a corrida (+3d). Enquanto, no grupo II as amostras foram coletadas oito dias antes da corrida (-8d), imediatamente após a corrida (0d), um dia após a corrida (+1d) e cinco dias após a corrida (+5d). Foram realizados ensaios de fagocitose e burst oxidativo dos neutrófilos, determinação de cortisol sérico, ensaios de apoptose, linfoproliferação e hemograma. A função de fagocitose, tanto em porcentagem, quanto em intensidade, mostrou-se diminuída nas amostras coletadas imediatamente após a corrida, nos dois grupos de animais. Os valores médios de burst oxidativo das amostras coletadas imediatamente após a corrida, dos animais do grupo I apresentaram-se diminuídos. Não foram encontradas diferenças estatísticas significantes entre os resultados obtidos em relação às células apoptóticas, quando os diferentes momentos foram comparados; o mesmo ocorreu quando foi realizado o confronto dos valores do ensaio de linfoproliferação. Os valores médios dos níveis séricos de cortisol apresentaram-se aumentados no momento imediatamente após a corrida, em relação aos valores dos outros momentos, nos dois grupos de animais. Quanto aos dados de hemograma, houve aumento do número percentual de neutrófilos no momento imediatamente após a corrida quando foi realizada comparação com os demais momentos. Nos cavalos estudados, ainda que tenha sido encontrada uma diminuição transitória na função dos neutrófilos, a função adaptativa não chegou a ser acometida, que sugere que, embora se constitua em um exercício de alta intensidade, a fugacidade da corrida acabe por evitar danos maiores, em animais bem condicionados e adaptados a situações de estresse / Although horses can present exceptional responses during stress situations in nature, it was raised the possibility that racehorses under certain stress conditions could develop changes that may affect their health and welfare. Alterations of the number and function of cells that are involved with the defense response, mainly neutrophils and lymphocytes populations were verified. Thirty Thoroughbred racehorses housed at the Jockey Club de São Paulo, in São Paulo, Brazil, were used in the present study. The animals were divided in two groups and blood samples were collected at different moments of their routine. In group I, the samples were collected eleven days before race (-11d), immediately after race (0d), one day (+1d), two days (+2d) and three days after race (+3d). In group II, the samples were collected eight days before race (-8d), immediately after race (0d), one day (+1d) and five days after race (+5d). Phagocytosis assay and oxidative burst activity of neutrophils, serum cortisol determination, apoptosis and lymphocyte proliferation assay and haematology tests were performed. The percentage and intensity of phagocytosis functions decreased in samples collected immediately after race, in both groups of animals. The mean values of oxidative burst activity of samples collected immediately after race, from animals of group I also decreased. Statistically significant differences between the results were obtained in relation to the apoptotic cells, when compared on different moments and the same occurred in Relation to the lymphocyte proliferation values. The mean values of serum cortisol levels increased at the moment immediately after race when compared with the values of the other moments, in both groups of animals. In relation to the haematologycal values, the percentage of neutrophils was increased at the moment immediately after race when compared with the other moments. Even though a transitory reduction in the neutrophils function has occurred, the adaptive function was not impaired, which suggest that, in spite of the high intensity of this exercise, the short-term race can prevent further damages in well-conditioned and stress-adapted animals

Cardiovascular diseases (CVD) are the leading cause of death, and the underlying atherosclerotic process has its origin in youth. Physical activity lowers future CVD risk, however few adolescents achieve the recommended minimum amount of daily activity and interventions fail to meaningfully increase activity levels in this group. It is therefore essential to identify how small volumes of exercise can be optimised for the primary prevention of CVD. The purpose of this thesis is to identify the influence of exercise intensity on vascular health outcomes in adolescents, and to assess the efficacy of 2 weeks of low volume, high-intensity interval training on CVD risk factors in this population. Chapter 4 demonstrates that a single bout of high-intensity interval exercise (HIIE) performed one hour before a high fat meal elicits comparable reductions in postprandial lipaemia as a work-matched bout of moderate-intensity exercise (MIE) in girls. However, neither exercise attenuated postprandial lipaemia in the boys. Additionally, HIIE elicited a superior increase in postprandial fat oxidation and decrease in blood pressure, and this was sex independent. These findings are furthered in Chapter 5, which identified that accumulating HIIE, but not MIE, favourably modulates glycaemic control, postprandial blood pressure and fat oxidation in adolescents irrespective of sex. A high fat meal was included in Chapter 6 in order to impair vascular function via oxidative stress. Postprandial vascular function was preserved following MIE, but improved after HIIE, and these changes were not related to changes in postprandial lipaemia or total antioxidant status. Chapter 7 addressed the time course of the changes in vascular function post exercise, and identified that HIIE promotes superior changes in vascular function than MIE. Finally, Chapter 8 identified that 2 weeks of high-intensity interval training improved novel (endothelial function and heart rate variability), but not traditional CVD factors in adolescent boys and girls. However, most of these favourable changes were lost 3 days after training cessation. Thus, this thesis demonstrates that vascular health outcomes are positively associated with exercise intensity. Given that HIIE was perceived to be more enjoyable than MIE in Chapters 4, 6 and 7, performing HIIE appears to be an effectual and feasible alternative to MIE for the primary prevention of CVD.

The high intensity exercise produces physiological, neural and cognitive benefits. Caffeine has been considered an important ergogenic resource in exercise, due to its ability to block adenosine receptors. Due to fact the mechanisms related to effect of caffeine on physical exercise has not yet been fully elucidated, in this study we evaluated the effects of caffeine treatment alone or in association with high intensity exercise on the enzymes NTPDase, 5´- nucleotidase, adenosine deaminase (ADA) and acetylcholinesterase (AChE) in brain of rats. Animals were divided into six groups: I (Control untrained); II (untrained plus caffeine 4 mg/kg); III (untrained plus caffeine 8 mg/kg); IV- (only trained) V- (trained plus caffeine 4 mg/Kg) and VI (trained plus caffeine 8 mg/Kg). Swimming was used as a model of intermittent exercise. Animals were trained three times a week, gradually increasing the workload up to 2.5% of the animal's body weight per week. Caffeine was administered by gavage one hour before the training for 5 days a week. Animals were submitted to a period of six weeks to protocol exercise and caffeine treatment. Results showed no alterations in the ATP (10,14%), ADP (23,2%), AMP (45,89%) hydrolysis and ADA (27,33) activity in synaptossomes of cerebral cortex of trained rats (Grupo IV). However, when trained rats were treated with caffeine we observed a significant increase in the ADP, AMP hydrolysis and ADA activity in synaptosomes of the cerebral cortex (V and VI) when compared to other groups (P<0.05). In relation to the AChE activity, we observed a decrease in the activity of this enzyme in synaptosomes from cerebral cortex in the trained group (IV) and in the animals from the groups V and VI when compared to the control group (P <0.05). Exercise alone did not alter the AChE activity in homogenates from cerebral cortex, striatum, cerebellum or hippocampus (group IV). Group V showed a decrease in the AChE activity in hippocampus and an increase in the activity of this enzyme in striatum and cerebral cortex in relation to control (P<0.05). In the animals from group VI, caffeine increased the AChE activity in cerebral cortex and in cerebellum in relation to other groups (P<0.05). These findings suggest that caffeine modulates the purinergic and cholinergic signaling associated with high intensity exercise. / O exercício físico de alta intensidade produz benefícios fisiológicos, cognitivos e neurais. A cafeína tem sido considerada um recurso ergogênico associada ao exercício, devido a sua capacidade de bloquear os receptores de adenosina. Devido ao fato dos mecanismos relacionados ao efeito da cafeína no exercício físico ainda não serem totalmente elucidados, o objetivo desse estudo foi avaliar o efeito da cafeína per se ou em associação ao exercício de alta intensidade na atividade das enzimas NTPDase, 5´-nucleotidase, adenosina desaminase (ADA) e acetilcolinesterase (AChE) em encéfalo de ratos. Os animais foram divididos em seis grupos: I (controle/sedentário); II (sedentário tratado com cafeína 4mg/kg); III (sedentário tratado com cafeína 8mg/kg); IV (treinado); V(treinado e tratado com cafeína 4mg/kg) e VI (treinado e tratado com cafeína 8mg/kg). A natação com adição de sobrecarga ao dorso de ratos foi utilizada como modelo de exercício sendo que os ratos foram treinados 3 vezes por semana com aumentos progressivos (2,5% do peso corporal) a cada semana até 23% do peso corporal. Cafeína foi administrada durante cinco dias na semana por gavagem uma hora antes do exercício. Os ratos foram submetidos ao tratamento com cafeína associado ao protocolo de exercício durante seis semanas. Os resultados demonstraram que não houve alterações na hidrólise do ATP (10,14%), ADP (23,2%) e AMP (45,89%) bem como na atividade da ADA (27,33%) em sinaptossomas de córtex cerebral do grupo treinado (IV). Quando ratos treinados foram tratados com cafeína ocorreu um aumento na hidrólise do ADP, AMP e na atividade da ADA em sinaptossomas de córtex cerebral (grupos V e VI) (P<0.05). Em relação a AChE, uma diminuição aproximada de 40% na atividade dessa enzima ocorreu em sinaptossomas de córtex cerebral de ratos treinados e/ou tratados com cafeína (grupo IV, V e Vl) (P<0.05). O exercício per se não alterou a atividade da AChE em homogeneizado de córtex cerebral, estriado, cerebelo e hipocampo (grupo IV). Nos ratos do grupo V foi observado uma diminuição na atividade da AChE em hipocampo e um aumento em estriado e córtex cerebral (P<0.05) enquanto que nos animais do grupo VI ocorreu um aumento na atividade da AChE em córtex cerebral e cerebelo quando comparado com outros grupos (P<0.05). Esses resultados sugerem que a cafeína per se ou associada ao exercício de alta intensidade modula a sinalização purinérgica e colinérgica em ratos.