Made available in DSpace on 2014-12-17T15:32:16Z (GMT). No. of bitstreams: 1 PedroPAS.pdf: 6126862 bytes, checksum: 3ae3ec2483d9e09d3a05fcd73782e862 (MD5) Previous issue date: 2008-02-27 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The giant cell fibroma is a benign neoplasm characterized by the presence of mono, bi or multinucleate cells, which can have a connection to the presence of mast cells. This research aims to analyze, descriptively and comparatively, the immunohystochemistry expression of the tryptase in mast cells of the giant cell f ibroma, f ibrous hyperplasia and samples of the normal oral mucosa. Thirty cases of giant cell fibroma, ten cases of fibrous hyperplasia and ten cases of normal oral mucosa were selected for the analysis of the immunohistochemistry expression, determination of the number of present mast cells, as well as their location and shape. It could be stated that there was a statistically signif icant difference (p<0,001) in relation to the quantity of mast cells among other samples analyzed where the giant cell f ibroma presented lesser quantity of mast cell and the hyperplasia showed higher concentration of this cellular type. Although the oral mucosa has presented a higher quantity of mast cells when compared to the giant cells fibroma, these were found in usual locations in the connective tissue in normal tissues. There could be noticed a statistically significant difference in relation to the number of non-granulated mast cells (p<0,001). On the areas of fibrosis, we could observe a statistically signif icant difference (p<0,006) among the samples. In relation to the present mast cells in perivascular location, no statistically signif icant difference was found. On the morphological analysis there was a predominance of oval mast cells. It was concluded that despite of the fact there was a lesser quantity of mast cells present in cases of giant cell f ibroma, they appeared to have a stronger relation to the present giant fibroblasts in this lesions, around 59,62%, being also evidenced a strong relation between these cells and the fibrosis areas in both cases of giant cell f ibroma and f ibrous hyperplasias and samples of normal oral mucosa, used as control group in our study, confirming, this way, the role of the mast cells as fibrinogenous inductor / O fibroma de c?lulas gigantes constitui-se de uma neoplasia benigna, caracterizada pela presen?a de c?lulas gigantes, mono, bi ou multinucleadas, c?lulas estas que podem guardar rela??o com a presen?a de mast?citos. O prop?sito desta pesquisa consistiu em analisar descritiva e comparativamente a express?o imuno-histoqu?mica da triptase em mast?citos de fibroma de c?lulas gigantes, hiperplasia fibrosa e esp?cimes de mucosa oral normal. Foram selecionados 30 casos de fibroma de c?lulas gigantes, 10 casos de hiperplasia fibrosa e 10 casos de mucosa oral normal, para a an?lise da express?o imuno-histoqu?mica, determina??o do n?mero de mast?citos presentes, bem como a sua forma e localiza??o. Constatou-se diferen?a estatisticamente significativa (p<0,001) em rela??o a quantidade de mast?citos entre os esp?cimes analisados, onde o fibroma de c?lulas gigantes apresentou a menor quantidade de mast?citos e a hiperplasia exibiu a maior concentra??o deste tipo celular. Embora a mucosa oral tenha apresentado uma maior quantidade de mast?citos quando comparado com os casos de f ibroma de c?lulas gigantes, estes se encontravam em localiza??es usuais no tecido conjuntivo em tecidos normais. Verif icou-se, diferen?a estatisticamente significativa, no que diz respeito ao n?mero de mast?citos n?o degranulados (p<0,001). Nas ?reas de fibrose, observamos diferen?a estatisticamente signif icativa (p<0,006) entre os esp?cimes. Com rela??o aos mast?citos presentes em localiza??o perivascular n?o se observou diferen?a estatisticamente significativa. Na an?lise morfol?gica verif icou-se uma predomin?ncia de mast?citos ovais. Concluiu-se que embora uma menor quantidade de mast?citos estivesse presente nos casos de fibroma de c?lulas gigantes, estes exibiam maior rela??o com os fibroblastos gigantes presentes nestas les?es em torno de 59,62%, sendo evidenciada tamb?m uma forte rela??o entre estas c?lulas e ?reas de fibrose tanto nos casos de fibroma de c?lulas gigantes como de hiperplasias fibrosas e esp?cimes de mucosa oral normal, utilizados como controle em nosso estudo, confirmando desta forma, o papel dos mast?citos como indutor fibrinog?nico

Made available in DSpace on 2014-12-17T15:32:21Z (GMT). No. of bitstreams: 1 PedroCRN_DISSERT.pdf: 3117630 bytes, checksum: 2544a3022fe67731388c2238d7dc5289 (MD5) Previous issue date: 2012-02-29 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Odontogenic cysts are pathologic cavities covered by odontogenic epithelium and filled by liquid, desquamated cells or other materials. The intraosseous lesions, such as radicular cyst and dentigerous cyst, present a potential of expansion capable of promoting the destruction of the surrounding osseous tissue. The mechanisms related to this process of expansion are the proliferation of cystic epithelium, the increase of the osmolarity of the cystic fluid and the synthesis of reabsorption factors such as IFN-? and TGF-?1. The aim of this study was to evaluate and compare the immunohistochemical expression of IFN-? and TGF-?1 between radicular cysts and dentigerous cysts in order to understand the role and behavior of these proteins in the expansion of these cysts. We selected 20 cases of radicular cyst and 20 cases of dentigerous cyst chosen from the files of UFRN s Laboratory of Oral Pathology. After analyzing the clinical data, the cases underwent the routine staining technique (HE) and immunohistochemistry for the appearance of IFN-? and TGF-?1 in the epithelium and capsule of these cysts. The statistical analysis using the Mann-Whitney test revealed no statistically significant difference in immunoexpression of IFN-? between the epithelium (p = 0.565) and capsules (p = 0.414) of radicular cysts and dentigerous cysts. Moreover, there was no statistically significant difference of immunoexpression of TGF-?1 between the epithelium (p = 0.620) and capsules (p = 0.056) of radicular cysts and dentigerous cysts. The Wilcoxon test revealed no statistically significant difference between IFN-? and TGF-?1 imunoexpressions in the epithelium (p = 0.225) and capsules (p = 0.370) of radicular cysts. There was no statistically significant difference between IFN-? and TGF-?1 imunoexpressions in the epithelium (p = 0.361) of dentigerous cysts. However, there was a statistically significant difference between IFN-? and TGF-?1 immunoexpressions in the capsule (p = 0.001) of dentigerous cysts, being TGF-?1 the factor which presented the most significant immunoexpression. Given these results, we conclude that there was no difference in immunohistochemical expression of IFN-? and TGF-?1 between radicular and dentigerous cysts and that TGF-?1 was more significant than the IFN-? in the capsule of dentigerous cysts / Os cistos odontog?nicos s?o cavidades patol?gicas revestidas por epit?lio odontog?nico e preenchidas por l?quido, c?lulas descamadas, ou outros materiais. As les?es intra-?sseas, como o cisto radicular e o cisto dent?gero, apresentam um potencial de expans?o capaz de promover a destrui??o do tecido ?sseo circunjacente. Os mecanismos relacionados a esse processo de expans?o s?o a prolifera??o do epit?lio c?stico, o aumento da osmolaridade do fluido c?stico e a s?ntese de fatores de reabsor??o ?ssea como IFN-? e TGF-?1. O objetivo deste estudo foi avaliar e comparar a express?o imuno-histoqu?mica do IFN-? e do TGF-?1 entre cistos radiculares e cistos dent?geros com a finalidade de compreender o papel e o comportamento dessas prote?nas no processo de expans?o destes cistos. Selecionamos 20 casos de cisto radicular e 20 casos de cisto dent?gero retirados dos arquivos do Laborat?rio de Patologia Oral da UFRN. Ap?s an?lise dos dados cl?nicos, os casos foram submetidos a t?cnica de colora??o de rotina (HE) e ao m?todo imuno-histoqu?mico para evidencia??o da express?o do IFN-? e do TGF-?1 no epit?lio e na c?psula dos referidos cistos. A an?lise estat?stica dos dados utilizando o teste de Mann-Whitney revelou que n?o houve diferen?a estatisticamente significativa da imunoexpress?o do IFN- entre os epit?lios (p=0,565) e c?psulas (p=0,414) dos cistos radiculares e cistos dent?geros. Al?m disso, n?o houve diferen?a estatisticamente significativa da imunoexpress?o do TGF-?1 entre os epit?lios (p=0,620) e c?psulas (p=0,056) dos cistos radiculares e cistos dent?geros. O teste de Wilcoxon revelou que n?o houve diferen?a estatisticamente significativa entre as imunoexpress?es do IFN- e do TGF-?1 no epit?lio (p=0,225) e na c?psula (p=0,370) dos cistos radiculares. N?o houve diferen?a estatisticamente significativa entre as imunoexpress?es do IFN- e do TGF-?1 no epit?lio (p=0,361) dos cistos dent?geros. No entanto, houve diferen?a estatisticamente significativa entre as imunoexpress?es do IFN- e do TGF-?1 na c?psula (p=0,001) dos cistos dent?geros, sendo o TGF-?1 o que apresentou a imunoexpress?o mais significativa. Diante destes resultados, conclu?mos que n?o houve diferen?a de express?o imuno-histoqu?mica do IFN-? e do TGF-?1 entre os cistos radiculares e dent?geros e que o TGF-?1 foi mais expressivo do que o IFN-? na c?psula dos cistos dent?geros

Made available in DSpace on 2014-12-17T15:32:22Z (GMT). No. of bitstreams: 1 MarceloABN_DISSERT.pdf: 4711000 bytes, checksum: ea3602bfcefe0d8448e9d402030634a2 (MD5) Previous issue date: 2014-02-14 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / BMPs are components superfamily ligands transformation growth fator-&#946; (TGF-&#946;) secreted into the extracellular environment, with mechanisms of intercellular communication through specific ligands and receptors in various target cells, being recognized for its influence in osteogenic induction, also play an important role in tissue homeostasis, cell proliferation, differentiation control , in addition to being present in the development of various malignancies. The aim of this study was to compare the immunohistochemical expression of BMP-2, BMP-4 and its receptors BMPRIA and BMPRII in cases of ameloblastoma and adenomatoid odontogenic tumor. The sample consisted of 20 cases of solid ameloblastoma (SA), 10 cases of ameloblastoma unicystic (UA) and 16 cases of adenomatoid odontogenic tumor (AOT). The expression of BMPs and their receptors was evaluated in the parenchyma and stroma of lesions, establishing the percentage of immunopositive cells (0 - negative; 1-1 % to 10 % of cells positive; 2 - 11% to 25% of positive cells; 3 - 26% to 50% of cells positive; 4 - 51% to 75 % of positive cells; 5 - more than 75% positive cells). Analysis of the expression of BMP-2 revealed no statistically significant differences in parenchymal (p = 0.925) and stromal component (p = 0.345) between the groups, as well as BMP-4 (p = 0.873 / p = 0.131). In the epithelial component, SA and AOT had a higher frequency of score 5. In turn, all cases of UA were classified as score 5. The analysis of the stromal component showed no statistically significant difference between groups with respect to median scores BMPRIA positivity (p = 0.768) and BMPRII (p = 0.779). In the epithelial component of SA and UA, no statistically significant correlations between imunoexpression proteins analyzed were observed. In turn, the group of AOT, statistically significant positive correlations between the scores of expression of all studied proteins were found. In the stromal component, statistically significant positive correlations were found only in the SA group in BMP -4 and BMPRII (r = 0.476; p = .034), in the UA in BMP-4 and BMPRIA (r = 0.709; p = 0.022). The results of this study suggest that the BMPs and their receptors are involved in the development process odontogenic tumors. BMP-4, in turn, besides being present in odontogenic tumors have the capacity to form mineralized material. / As BMPs s?o componentes da superfam?lia de ligantes do fator transformador de crescimento-&#946; (TGF-&#946;), secretados no meio extracelular, com mecanismos de comunica??o intercelular por meio de ligantes e receptores espec?ficos em diversas c?lulas-alvo, sendo reconhecidas por sua influ?ncia na indu??o osteog?nica, tamb?m desempenhando importante papel na homeostase tecidual, prolifera??o celular, no controle de diferencia??o, al?m de estar presente no desenvolvimento de diversas neoplasias. O objetivo deste estudo foi comparar a express?o imuno-histoqu?mica da BMP-2, BMP-4 e seus receptores BMPRIA e BMPRII em casos de Ameloblastoma e Tumor odontog?nico adenomat?ide. A amostra foi constitu?da de 20 casos de Ameloblastoma s?lido (AS), 10 casos de Ameloblastoma unic?stico (AU) e 16 casos de Tumor odontog?nico adenomat?ide (TOA). A express?o das BMPs e seus receptores foi avaliada no par?nquima e estroma das les?es, estabelecendo-se o percentual de c?lulas imunopositivas (0 negativo; 1 - 1% a 10% das c?lulas positivas; 2 - 11% a 25% das c?lulas positivas; 3 - 26% a 50% das c?lulas positivas; 4 - 51% a 75% das c?lulas positivas; 5 - mais 75% de c?lulas positivas). A an?lise da express?o de BMP-2 n?o revelou diferen?as estatisticamente significativas no componente par?nquimatoso (p = 0,925) e estromal (p = 0,345) entre as les?es estudadas, assim como a BMP-4 (p = 0,873 / p = 0,131). No par?nquima, o AS e TOA apresentaram maior frequ?ncia do escore 5. Por sua vez, todos os casos de AU foram classificados como escore 5. A an?lise do componente estromal revelou n?o haver diferen?a estatisticamente significativa entre os grupos em rela??o ?s medianas dos escores de positividade para BMPRIA (p = 0,768) e BMPRII (p = 0,779). No par?nquima do AS e do AU, n?o foram observadas correla??es estatisticamente significativas entre as imunoexpress?es das prote?nas analisadas. Por sua vez, no grupo dos TOAs, foram constatadas correla??es positivas, estatisticamente significativas, entre os escores de express?o de todas as prote?nas avaliadas. No componente estromal, foram constatadas correla??es positivas, estatisticamente significativas, apenas no grupo do AS em BMP-4 e BMPRII (r = 0,476; p = 0,034) e do AU em BMP-4 e BMPRIA (r = 0,709; p = 0,022). Os resultados do presente estudo sugerem que as BMPs e seus receptores est?o envolvidos no processo de desenvolvimento de tumores odontog?nicos. A BMP-4, por sua vez, al?m de estar presente em tumores odontog?nicos possui a capacidade de forma??o de material mineralizado

Made available in DSpace on 2014-12-17T15:32:27Z (GMT). No. of bitstreams: 1 RobertaBC.pdf: 1065704 bytes, checksum: 6e6f9aa3d97c1150d7b5fd4167469597 (MD5) Previous issue date: 2008-08-30 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Pleomorphic adenoma and adenoid cystic carcinoma represent a benign and malignant salivary gland neoplasm, respectively, that shares the same histological origin, however with distinct biological behavior. The aim of the present study was identify the -160 C/A polymorphism in the gene CDH1, mutational analysis of CTNNB1 gene and evaluation the expression of the E-cadherin and ?-catenin in pleomorphic adenomas and adenoid cystic carcinomas. Furthermore, it was proposed correlate the immunochemistry staining patterns with the polymorphism and mutations. Twenty-four pleomorphic adenomas and 24 adenoid cystic carcinomas were retrieved. The polymorphism analysis was performed by restriction fragment length polymorphism (RFLP), using the restriction enzymes HphI or AflIII and the mutational screening was performed by PCR-single strand conformational polymorphism (PCR-SSCP). The immunohistochemical analysis was taken by the counting of cells, recorded as the Hscore index, and considering the presence or absence, intensity, distribution and localization of proteins expression. Comparing the two neoplasms, the results demonstrated statistically significant difference for the E-cadherin and ?-catenin expression, with pleomorphic adenoma presenting weaker immunostaining. Was observed statistical correlation between E-cadherin and ?-catenin expression. CDH1 heterozigotic polymorphism was seen in two cases and 13 cases displayed abnormal mobility electrophoretic shifts, suggesting CTNNB1 gene mutation. The immunohistochemical expression was not statistically correlated with the polymorphism or suggested mutations. In conclusion this study supports that the E-cadherin/?-catenin complex immunohistochemical expression might be related with the myoepithelial component amount and differentiation neither the tumor biological behavior. The cases that showed E-cadherin gene polymorphism presented reduced protein expression and, moreover, CTNNB1 suggested mutations seem not influence in the ?-catenin protein expression / O adenoma pleom?rfico e o carcinoma aden?ide c?stico representam neoplasias de gl?ndula salivar benigna e maligna, respectivamente, que compartilham a mesma origem histol?gica, por?m com comportamentos biol?gicos distintos. O prop?sito deste estudo consistiu na identifica??o do polimorfismo -160 C/A da regi?o promotora do gene CDH1 (E-caderina), na triagem de muta??es no gene CTNNB1 (?-catenina), e ainda na an?lise da express?o imuno-histoqu?mica das prote?nas E-caderina e ?-catenina em adenomas pleom?rficos e carcinomas aden?ides c?sticos. Al?m disso, objetivou-se correlacionar os achados imuno-histoqu?micos com as poss?veis muta??es e polimorfismo. Foram selecionados 24 casos de adenoma pleom?rfico e 24 casos de carcinoma aden?ide c?stico. Para a identifica??o do polimorfismo no gene da E-caderina empregou-se a t?cnica RFLP (restriction fragment length polymorphism) utilizando-se enzimas de restri??o HphI e AflIII. A triagem de muta??es no exon 3 do gene da ?-catenina foi realizada por meio de SSCP (single strand conformational polymorphism). Para a an?lise imuno-histoqu?mica, procedeu-se contagem de c?lulas, por meio do ?ndice HScore e verificou-se presen?a ou aus?ncia, intensidade, padr?o de distribui??o e localiza??o celular e tecidual das prote?nas. Os resultados demonstraram diferen?a estatisticamente significativa quando a marca??o imuno-histoqu?mica, tanto da E-caderina quanto ?-catenina, foi comparada entre as duas neoplasias estudadas, apresentando o adenoma pleom?rfico express?o reduzida. Observou-se correla??o estatisticamente significativa entre a imuno-marca??o da E-caderina e ?-catenina. Dois casos (1 adenoma pleom?rfico e 1 carcinoma aden?ide c?stico) apresentaram polimorfismo heterozig?tico no gene CDH1 e 13 casos (6 adenomas pleom?rficos e 7 carcinomas aden?ides c?sticos) exibiram varia??o no padr?o de corrida eletrofor?tica, sugerindo muta??o do gene CTNNB1. N?o houve correla??o estatisticamente significativa entre a marca??o imuno-histoqu?mica e presen?a de polimorfismo ou poss?veis muta??es. Conclui-se que a express?o imuno-histoqu?mica do complexo E-caderina/?-catenina pode estar relacionada com a quantidade e diferencia??o do componente mioepitelial e n?o ao comportamento biol?gico dos tumores. Os casos que exibiram polimorfismo no gene da E-caderina apresentaram redu??o na express?o prot?ica e, por fim, as poss?veis muta??es no gene CTNNB1 parecem n?o influenciar na express?o da prote?na ?-catenina

Made available in DSpace on 2014-12-17T15:32:28Z (GMT). No. of bitstreams: 1 JanainaCL.pdf: 1946389 bytes, checksum: 9edf9a3e8f8e033293bf82a3b2344d4d (MD5) Previous issue date: 2009-02-28 / Host response plays a major role in the pathogenesis of periodontal disease. Mediators such as inflammatory cytokines which are secreted during the immune response to bacterial challenges have ambiguous functions that may or may not lead to protection of the attacked tissue. In this context, experimental evidence suggests that T-helper 1 (Th-1) and T-helper 2 (Th-2) mediated responses are potentially important during the disease process. The aims of this study therefore were to further clarify the role played by Th2 cells during different time points of the active phase of periodontal disease, as well as, to investigate whether there was any evidence of a Th1 response in the periodontal disease microenvironment. Experimental periodontitis was induced in 30 Wistar male rats by placing cotton ligatures around the mandibular first molars. The rats were then randomly divided into two groups. Group1 (G1=15) and Group 2 (G2=15). In G1 the ligatures were maintained for 2 days, whereas in G2 the ligatures were left for 15 days, a time point that corresponds to the advanced stage of periodontal disease The contra-lateral teeth served as controls (no ligatures). Immunohistochemical investigation for the presence in gingival tissue of Th2 specific transcription factor (GATA3) and the subunit of the IFN-&#947; receptor was carried out after the disease induction period. Light microscopy analysis revealed a decrease in the expression of GATA-3 as bone loss progressed. On the other hand, although IFN-&#947; R1 was detected at an early stage of the active phase of disease its expression remained unaltered during the remaining period of the study. These results indicate that the Th2 response have a protective role during the pathogenesis of periodontal disease and that the progression of the periodontal disease is related with the unbalance of the responses Th1/Th2 / A resposta do hospedeiro tem um importante papel na patog?nese da doen?a periodontal. Mediadores como as citocinas liberadas por c?lulas inflamat?rias durante a resposta imune, frente a um ataque bacteriano, desempenham pap?is antag?nicos que podem culminar com a prote??o ou n?o do tecido agredido, o que fundamenta o paradigma da resposta Th1/Th2 na doen?a periodontal. Na tentativa de esclarecer a participa??o das c?lulas Th2, em diferentes tempos, na fase ativa da doen?a periodontal, bem como observar se o microambiente encontrava-se preparado para uma resposta Th1, induziu-se doen?a periodontal experimental em 30 ratos Wistar machos, atrav?s da coloca??o de ligaduras de algod?o ao redor dos primeiros molares mandibulares. Os animais foram divididos, aleatoriamente, em dois grupos: Grupo 1 (G1 = 15) e Grupo 2 (G2 = 15). Em G1 as ligaduras foram mantidas por 2 dias, que configurou o est?gio inicial da doen?a periodontal e no G2, as ligaduras foram mantidas por 15 dias, que foi considerado o est?gio avan?ado da doen?a periodontal. Os dentes contralaterais serviram de controle (sem ligaduras). Avalia??o imuno-histoqu?mica para o fator de transcri??o espec?fico para c?lula Th2 (GATA-3) e da subunidade IFN- &#947; R1 do receptor para IFN-&#947; (principal citocina da resposta Th1) foram avaliados nos tecidos gengivais dos ratos ap?s o per?odo de indu??o da doen?a. Ap?s an?lise microsc?pica observamos que o fator de transcri??o GATA-3 teve sua express?o diminu?da com o avan?o da perda ?ssea induzida e a subunidade IFN-&#947; R1 passou a se expressar na fase ativa da doen?a experimental, contudo n?o se alterou com a progress?o da destrui??o tecidual. Estes resultados indicam que a resposta Th2 pode estar associada a uma resposta protetora na patog?nese da doen?a periodontal e que a progress?o da doen?a periodontal est? relacionada com o desequil?brio das respostas Th1/Th2

Made available in DSpace on 2014-12-17T15:32:30Z (GMT). No. of bitstreams: 1 MarceloGV_TESE.pdf: 4128129 bytes, checksum: ebce1d2816c557119bfc6ec3a006f620 (MD5) Previous issue date: 2011-11-18 / The tumor hypoxia modulates a series of genetic changes related to adaptive development, invasion and metastasis of various human cancers, among which squamous cell carcinoma of the tongue (SCCT). The objective of this study was to analyze clinical, morphological and immunohistochemical expression by HIF-1&#945;, GLUT-1 and CA-IX in 57 cases of CEL and correlated this expression to clinical parameters and morphological. After a descriptive analysis of data on gender, age, race, and habits of patients, it was found that the results were consistent with the literature. The clinical and morphological parameters analyzed and the expression of these markers of hypoxia were subjected to statistical analysis (Qui2 test), verifying that they can be used as indicators of the biological behavior of CEL. Among the results of this study, we observed that the intensity of expression for HIF-1&#945;, in most cases located in the cytoplasm and nucleus, statistically correlated with clinical staging (p = 0.011) and histological grading (p = 0.002). As for the relationship between the distribution of labeling for HIF-1&#945; and metastasis, the chi-square (Qui2) showed that there was statistically significant differences between the groups (p = 0.040). 75.8% of the sample who had metastases, there was the predominance of diffuse marking. The immunoexpression cytoplasmic/membrane GLUT-1 showed a statistically significant correlation with the clinical stage (p = 0.002) and histological grading (p = 0.000). Concerning the location of markings for GLUT-1 tumor on the island, there was a predominance of peripheral marking specimens in most low-grade (78.6%). In the sample of high-grade, prevailed the location center/periphery (55.8%). According to the chi-square (Qui2), the location on the island of the tumor (p = 0.025) showed statistically significant difference in histological grading. The immunoreactivity of CA-IX, in most cases located in the membrane and cytoplasm, exhibited a statistically significant correlation with histological grading (p = 0.005). Based on these results, we can conclude a broad participation of these markers of hypoxia in oral carcinogenesis and its possible use as markers of biological behavior and tumor progression in CEL / A hip?xia tumoral modula uma s?rie de mudan?as gen?ticas adaptativas relacionadas ao desenvolvimento, invas?o e met?stase de diversos c?nceres humanos, dentre os quais o carcinoma epiderm?ide de l?ngua (CEL). O objetivo do presente trabalho foi realizar uma an?lise cl?nica, morfol?gica e imuno-histoqu?mica atrav?s da express?o do HIF-1&#945;, GLUT-1 e da CA-IX em 57 casos de CEL, correlacionando essa express?o ? par?metros cl?nicos e morfol?gicos. Ap?s uma an?lise descritiva dos dados referentes ao sexo, faixa et?ria, ra?a e h?bitos dos pacientes, constatou-se que os resultados encontrados foram condizentes com a literatura. Os par?metros cl?nicos e morfol?gicos analisados e a express?o desses marcadores de hip?xia foram submetidos ? an?lise estat?stica (teste do Qui2), verificando-se que os mesmos podem ser utilizados como indicadores do comportamento biol?gico do CEL. Dentre os resultados da presente pesquisa, observou-se que a intensidade de express?o para o HIF-1&#945;, localizada na maioria dos casos no citoplasma e n?cleo, correlacionou-se estatisticamente com o estadiamento cl?nico (p = 0,011) e grada??o histol?gica (p = 0,002). Quanto ? rela??o entre a distribui??o de marca??o para o HIF-1&#945; e met?stase, o teste qui-quadrado (Qui2) demonstrou haver diferen?as estatisticamente significativas entre os grupos analisados (p = 0,040). Dos 75,8% da amostra que tinham met?stase, constatou-se a o predom?nio da marca??o difusa. A imunoexpress?o citoplasm?tica/membranar do GLUT-1 exibiu uma correla??o estatisticamente significativa com o estadiamento cl?nico (p = 0,002) e grada??o histol?gica (p = 0,000). Em rela??o ? localiza??o de marca??o para o GLUT-1 na ilha tumoral, evidenciou-se predom?nio da marca??o perif?rica na maioria dos esp?cimes de baixo grau (78,6%). Na amostra de alto grau, prevaleceu a localiza??o centro/periferia (55,8%). De acordo com o teste qui-quadrado (Qui2), a localiza??o na ilha tumoral (p = 0,025) demonstrou haver diferen?as estatisticamente significativas com a grada??o histol?gica. A imunoexpress?o da CA-IX, localizada na maioria dos casos na membrana e citoplasma, exibiu uma correla??o estatisticamente significativa com a grada??o histol?gica (p = 0,005). Com base nestes resultados, pode-se concluir uma ampla participa??o desses marcadores de hip?xia na carcinog?nese oral, bem como a sua poss?vel utiliza??o como marcadores do comportamento biol?gico e da progress?o tumoral em CEL

Made available in DSpace on 2014-12-17T15:36:53Z (GMT). No. of bitstreams: 1 FrancimarAS.pdf: 4205737 bytes, checksum: a4c2113c8c56b274ed7600ae2a39f704 (MD5) Previous issue date: 2008-09-26 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The auditory system is composed by a set of relays from the outer ear to the cerebral cortex. In mammals, the central auditory system is composed by cochlear nuclei, superior olivary complex, inferior colliculus and medial geniculate body. In this study, the auditory rombencephalic centers, the cochlear nuclear complex and the superior olivary complex were evaluated from the cytoarchitecture and neurochemical aspects, thorough Nissl staining and immunohistochemical techniques to reveal specific neuron nuclear protein (NeuN), glutamate (Glu), glutamic acid decarboxilase (GAD), enkephalin (ENK), serotonin (5-HT), choline acetyltransferase (ChAT) and calcium-binding proteins calbindin (CB), calretinin (CR), and parvalbumin (PV). The common marmoset (Callithrix jacchus), a little native primate of the Brazilian atlantic forest was used as an experimental animal. As results, it was noted that the cochlear nuclear complex is composed by anteroventral, posteroventral and dorsal nuclei, and the superior olivary complex is constituted by the lateral and medial superior olivary nuclei and the trapezoid body nucleus. Glu, GAD, ENK, ChAT, CB, CR, PV-immunoreactive cells, fibers and terminals besides besides only 5-HT terminals were found unhomogeneously in all nuclei, of both complex. The emerging data are discussed in a comparative and functional context, and represent an important contribution to knowledge of the central auditory pathways in the common marmoset, and then in primates / O sistema auditivo compreende uma s?rie de esta??es que se estendem desde a orelha externa at? o c?rtex cerebral. Em mam?feros o sistema auditivo central subcortical ? formado essencialmente por n?cleos cocleares, complexo olivar superior, col?culo inferior e corpo geniculado medial. Neste estudo, os centros rombencef?licos, compreendendo o complexo nuclear coclear e o complexo olivar superior foram avaliados com rela??o a sua citoarquitetura e conte?do neuroqu?mico de corpos celulares e terminais ax?nicos, atrav?s das t?cnicas de colora??o de Nissl e imuno-histoqu?mica para prote?na nuclear neur?nio espec?fica (NeuN), glutamato (Glu), descaboxilase de ?cido glut?mico (GAD), encefalina (ENK), serotonina (5-HT), colina acetiltransferase (ChAT) e prote?nas ligantes de c?lcio calbindina (CB), cal-retinina (CR) e parvalbumina (PV). Foi utilizado como animal experimental o sag?i (Callithrix jacchus), um pequeno primata nativo da Mata Atl?ntica do Nordeste Brasileiro. Como resultados, foi evidenciado que o complexo nuclear coclear ? composto pelos n?cleos cocleares antero-ventral, p?stero-ventral e dorsal, e o complexo olivar superior pelos n?cleos olivares superiores lateral e medial e o n?cleo do corpo trapez?ide. Em todos os n?cleos, de ambos os complexos, foram encontrados de forma vari?vel corpos celulares, fibras e terminais imunorreativos a Glu, GAD, ChAT, CB, CR, PV, corpos celulares e terminais imunoreativos a ENK, al?m de fibras e terminais imunorreativos a 5-HT em diferentes densidades. Os dados obtidos s?o discutidos dentro de um contexto comparativo e funcional e representam uma importante contribui??o ao conhecimento das vias auditivas centrais no sag?i, e por extens?o em primatas

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-10-05T19:11:50Z No. of bitstreams: 1 CamilaCristinaGuimaraesNobre_DISSERT.pdf: 3735111 bytes, checksum: 1da00d1e44ef4ca4da5192f63cc4d5e6 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-10-17T21:08:41Z (GMT) No. of bitstreams: 1 CamilaCristinaGuimaraesNobre_DISSERT.pdf: 3735111 bytes, checksum: 1da00d1e44ef4ca4da5192f63cc4d5e6 (MD5) / Made available in DSpace on 2017-10-17T21:08:41Z (GMT). No. of bitstreams: 1 CamilaCristinaGuimaraesNobre_DISSERT.pdf: 3735111 bytes, checksum: 1da00d1e44ef4ca4da5192f63cc4d5e6 (MD5) Previous issue date: 2017-05-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O c?ncer do colo do ?tero ? o terceiro tipo de c?ncer mais frequente em mulheres no Brasil, sendo a sua frequ?ncia menor apenas do que aquelas observadas para o c?ncer de pele n?o-melanoma e c?ncer de mama. O fator inibit?rio da migra??o de macr?fagos (MIF) ? produzido por diferentes tipos de c?lulas e participa de uma cadeia complexa de eventos que favorece o processo de carcinog?nese, sendo poss?vel observar um alto n?vel de express?o em quase todos os tipos de c?ncer humano, entre eles o c?ncer do colo do ?tero. O MIF tamb?m induz um aumento dose dependente na excre??o do fator de crescimento de endot?lio vascular (VEGF), que promove a angiog?nese, o crescimento tumoral e a migra??o dessas c?lulas no c?ncer do colo do ?tero. O objetivo deste estudo foi investigar a express?o de MIF e VEGF em pacientes que apresentam ou n?o les?es do colo do ?tero, no intuito de identificar a exist?ncia de uma rela??o direta entre a presen?a dos marcadores MIF e VEGF com o grau da les?o da paciente, bem como, com a presen?a do papilomav?rus humano (HPV). Foram inclu?das no estudo 45 mulheres que haviam sido encaminhadas para a Maternidade Escola Janu?rio Cicco com suspeita de les?es na c?rvice uterina. As pacientes que aceitaram participar do estudo responderam a um question?rio, para obten??o de dados s?cio-demograficos, seguido de exame cl?nico. Das mulheres que se submeteram a colposcopia, foram coletados dois fragmentos de tecido do colo do ?tero, um para an?lise histopatol?gica e outro para detec??o do HPV por PCR convencional. A express?o dos biomarcadores, MIF e VEGF, foi detectada pela t?cnica de imuno-histoqu?mica. A ?rea positiva de cada biomarcador foi lida e quantificada utilizando o programa ImageJ?, e o resultado foi analisado atrav?s dos programas de estat?stica SPSS? Statistics e GraphPad Prism. Das 45 pacientes inclu?das no estudo, 20% apresentaram resultado do exame dentro dos padr?es da normalidade, enquanto que 80% apresentaram algum tipo de altera??o no exame; sendo 35,55% les?es intraepiteliais de baixo grau (LIEBG) e 44,44% les?es intraepiteliais de alto grau (LIEAG). A taxa de preval?ncia global de infec??o genital pelo HPV foi de 80%, sendo 86,1% em pacientes com les?o. A express?o m?dia do VEGF e do MIF tiveram um aumento gradativo quando comparado entre as pacientes normais, LIEBG e LIEAG, correspondendo respectivamente aos seguintes valores, 19,62, 41,59 e 55,42 para o VEGF e 4,36, 9,44 e 22,86 para MIF. Foi poss?vel verificar uma correla??o positiva entre a express?o de MIF e VEGF (r = 0,523, p = <0,001). Por meio deste trabalho p?de-se concluir que o VEGF e o MIF est?o correlacionados e envolvidos no processo de displasia cervical, aumentando a sua express?o conforme ocorre a progress?o da les?o. No entanto, n?o foi poss?vel encontrar associa??o entre a presen?a do HPV e os n?veis de MIF e VEGF. / The cervical cancer is the third most frequent type of cancer in women in Brazil, and its frequency is only lower than those observed for non-melanoma skin cancer and breast cancer. Macrophage migration inhibitory factor (MIF) is produced by different cell types and participates in a complex chain of events that favors the process of carcinogenesis, being possible to observe a high expression level in almost all types of human cancer, such as in cervical cancer. MIF also induces a dose-dependent increase in the excretion of vascular endothelial growth factor (VEGF), which promotes angiogenesis, tumor growth and migration of these cells in cervical cancer. The objective of this study was to investigate the expression of MIF and VEGF in patients with or without cervical lesions, in order to identify the existence of a direct relationship between the presence of those markers with the degree of the lesion of the patient, as well, as with the presence of human papillomavirus (HPV). The study included 45 women who had been referred to the Maternity School Janu?rio Cicco with suspected lesions of the uterine cervix. Patients who accepted to participate in the study answered a questionnaire to obtain socio-demographic data, followed by clinical examination. Patients who agreed to participate in the study answered a questionnaire to obtain socio-demographic data, followed by clinical examination. Two cervix tissue fragments were collected from the women who underwent colposcopy, one for histopathological analysis and the other for HPV detection by conventional PCR. The expression of the biomarkers, MIF and VEGF, was detected by the immunohistochemical technique. The positive area of each biomarker was read and quantified using the ImageJ? program and the result was analyzed using the statistical program SPSS? Statistics and GraphPad Prism. Of the 45 patients included in the study, 20% showed no lesions, while 80% had some type of alteration in the exam; being 35.55% low-grade squamous intraepithelial lesion (LSIL) type and 44.44% highgrade squamous intraepithelial lesion (HSIL) type. The overall prevalence rate of genital HPV infection was 80%, with 86.1% in patients with lesions. Mean VEGF and MIF expression increased gradually when compared to normal patients, LSIL and HSIL, respectively, corresponding to the following values: 19.62, 41.59 and 55.42 for VEGF and 4.36, 9, 44 and 22.86 for MIF. A positive correlation between MIF and VEGF expression was found (r = 0.523, p = <0.001). Through this work it was concluded that VEGF and MIF are correlated and involved in the cervical dysplasia process, increasing its expression as the lesion progresses. However, it was not possible to find an association between the presence of HPV and the levels of MIF and VEGF.

Made available in DSpace on 2015-03-03T15:38:43Z (GMT). No. of bitstreams: 1 PedroPAS_TESE_1-70.pdf: 4719637 bytes, checksum: 8f16cb0e2326a80cfc947b1ea2b89641 (MD5) Previous issue date: 2012-02-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Myofibroblasts are cells that exhibit a hybrid phenotype, sharing the morphological characteristics of fibroblasts and smooth muscle cells, which is acquired during a process called differentiation. These cells then start to express -SMA, a marker that can be used for their identification. Studies suggest that myofibroblasts are related to the aggressiveness of different tumors and that TGF-1 and IFN- play a role in myofibroblast differentiation, stimulating or inhibiting this differentiation, respectively. The objective of this study was to investigate the role of myofibroblasts in epithelial odontogenic tumors, correlating the presence of these cells with the aggressiveness of the tumor. Immunohistochemistry was used to evaluate the expression of TGF-1 and IFN- in myofibroblast differentiation, as well as the expression of MMP-13, which is activated by myofibroblasts, and of EMMPRIN (extracellular matrix metalloproteinase inducer) as a precursor of this MMP. The sample consisted of 20 solid ameloblastomas, 10 unicystic ameloblastomas, 20 odontogenic keratocysts, and 20 adenomatoid odontogenic tumors. For evaluation of myofibroblasts, anti- -SMA-immunoreactive cells were quantified in connective tissue close to the epithelium. Immunoexpression of TGF-1, IFN-, MMP-13 and EMMPRIN was evaluated in the epithelial and connective tissue components, attributing scores of 0 to 4. The results showed a higher concentration of myofibroblasts in solid ameloblastomas (mean of 30.55), followed by odontogenic keratocysts (22.50), unicystic ameloblastomas (20.80), and adenomatoid odontogenic tumors (19.15) (p=0.001). No significant correlation between TGF-1 and IFN- was observed during the process of myofibroblast differentiation. There was also no correlation between the quantity of myofibroblasts and MMP-13 expression. Significant correlations were found between MMP-13 and TGF-1 (r=0.087; p=0.011), between MMP- 13 and IFN- (r=0.348; p=0.003), as well as between EMMPRIN and MMP-13 (r=0.474; p<0.001) and between EMMPRIN and IFN- (r=0.393; p=0.001). The higher quantity of myofibroblasts observed in solid ameloblastomas, odontogenic keratocysts and unicystic ameloblastomas suggests that these cells are one of the factors responsible for the more aggressive biological behavior of these tumors, although the myofibroblast population was not correlated with TGF-1, IFN-, MMP-13 or EMMPRIN. The correlation between MMP- 13 and TGF-1 suggests that the latter induces the expression of this metalloproteinase. The present results also support the well-established role of EMMPRIN as an inducer of MMP-13. Furthermore, the relationship between EMMPRIN and IFN- and between MMP-13 and IFN- suggests synergism in the antifibrotic effect of these markers / Os miofibroblastos s?o c?lulas que apresentam um fen?tipo h?brido exibindo caracter?sticas morfol?gicas de fibroblastos e de c?lulas musculares lisas, sendo a aquisi??o de tal fen?tipo denominada diferencia??o, passando ent?o a expressar a -SMA, a qual ? importante na identifica??o dessas c?lulas. Estudos t?m sugerido que os miofibroblastos apresentam rela??o com a agressividade de diversas les?es e que o seu processo de diferencia??o estaria relacionado ? express?o do TGF- 1 e do IFN- atuando, respectivamente, no est?mulo e na inibi??o dessa diferencia??o. O objetivo deste trabalho foi investigar o papel dos miofibroblastos em les?es odontog?nicas epiteliais, relacionando-os ? agressividade das les?es e analisar por meio da imuno-histoqu?mica, a express?o do TGF- 1 e IFN- no processo de diferencia??o, al?m da an?lise da MMP-13 que ? ativada por miofibroblastos e do indutor de metaloproteinases de matriz (EMMPRIN) como precursor desta MMP. A amostra foi constitu?da por 20 ameloblastomas s?lidos, 10 ameloblastomas unic?sticos, 20 ceratocistos odontog?nicos e 20 tumores odontog?nicos adenomat?ides. Para a avalia??o dos miofibroblastos, foram quantificadas as c?lulas imunorreativas ao anticorpo - SMA presentes no tecido conjuntivo, pr?ximo ao tecido epitelial. As express?es de TGF- 1, IFN- , MMP-13 e EMMPRIN, foram avaliadas no componente epitelial e no conjuntivo, estabelecendo-se o percentual de imunorreatividade e atribuindo-se escores de 0 a 4. A an?lise dos miofibroblastos evidenciou maior concentra??o nos ameloblastomas s?lidos (m?dia de 30,55), seguido pelos ceratocistos odontog?nicos (22,50), ameloblastomas unic?sticos (20,80) e tumores odontog?nicos adenomat?ides (19,15) com valor de p= 0,001. N?o foi encontrada correla??o significativa entre TGF- 1 e IFN- no processo de diferencia??o dos miofibroblastos, bem como na rela??o entre a quantidade de miofibroblastos e a express?o da MMP-13. Constatou-se, correla??o estat?stica entre MMP-13 e TGF- 1 (r= 0,087; p= 0,011) al?m de significante correla??o entre MMP-13 e IFN- (r=0,348; p=0,003). Entre EMMPRIN e MMP-13 verificou-se signific?ncia (r= 0,474; p<0,001) assim como entre EMMPRIN e IFN- (r=0,393; p=0,001). A maior quantidade de miofibroblastos evidenciada nos ameloblastomas s?lidos, ceratocistos odontog?nicos e ameloblastomas unic?sticos sugere que estas c?lulas podem ser um dos fatores respons?veis para um comportamento biol?gico mais agressivo destas les?es, embora a popula??o de miofibroblastos n?o tenha apresentado correla??o com TGF- - 1, IFN- ,MMP-13 e EMMPRIN. Quanto a correla??o evidenciada entre MMP-13 e TGF- 1, isto pode sugerir um papel indutor do TGF- 1 para a express?o da MMP-13, assim como os resultados deste estudo refor?am a rela??o bem estabelecida do EMMPRIN como indutor da MMP-13. Constatou-se tamb?m rela??o entre EMMPRIN e IFN- assim como entre MMP-13 e IFN- sugerindo, dessa forma, um sinergismo na a??o anti-fibr?tica desses marcadores

Made available in DSpace on 2014-12-17T15:32:17Z (GMT). No. of bitstreams: 1 MarciaCCN.pdf: 387769 bytes, checksum: 0a9e8612244642f4c9e83714144b0e02 (MD5) Previous issue date: 2006-05-19 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Glandular atrophy is one of several alterations which can aflict the salivary glands, caused generally by obstructive lesions such as sialo1ithiasis, infections or compression by neoplastic processes arnong others. In this work, a morphological and immunohistochemical study was carried out in rat parotid glands, which were submitted to obstruction of the main excretory duct suffering atrophy at varied time intervals, with the aim of appraising the behavior of myoepithelial cells during the process of glandular atrophy. It was analized the immunohistochemical expression of calponin which detects myoepithelial cells in the parotids of 28 animaIs, which were divided into 7 groups, each one made up of 4 rats, afier the ductal ligature procedure, in the following time intervals: zero hour (control), 24 hours, 7, 15, 21, 30 and 60 days. Analysis of the immunohistochemicaI profile was carried through in which the calponin expression was veritied through its distribution pattem and numericaI index. All specimens exhibited positivity for calponin in myoepithelial cells which were distributed around the acini and the ductaI structures, a small number of positiveIy marked cells being detected in the controI group and in the 24-hour group when compared to subsequent ones, where it was perceived a Iarge increase in the number of positiveIy marked cens, mainly surrounding the ductiform structures which originated during the obstruction time. Upon application of statistical tests it was verified that the rise in the number the myoepithelial positive cells for calponin, when the control groups (zero hour) was compared to the 7, 15,21, 30 and 60-day groups afier obstruction, was statistica1ly significant. It was concluded then that the detected rise probably carne about due to an elevation in the rate of proliferation of the myoepitheliaI cells subsequent to the ductal obstruction, associated with a growing resistance of these cells to glandular atrophy. / A atrofia glandular ? uma das diversas altera??es que podem acometer as gl?ndulas salivares,causada geralmente por les?es obstrutivas como sialolit?ase,infec??es ou compress?o por processos neopl?sicos,dentre outros.No presente trabalho realizou-se estudo morfol?gico e imuno-histoqu?mico em gl?ndulas par?tidas de ratos submetidos ? obstru??o do ducto excretor principal,sofrendo atrofia em intervalos variados de tempo,visando avaliar o comportamento das c?lulas mioepiteliais durante o processo de atrofia glandular.Analisou-se a express?o imuno-histoqu?mica da calponina,a qual detecta c?lulas mioepiteliais,nas par?tidas de 28 animais divididos em 7 grupos com 4 ratos cada,ap?s o procedimento de ligadura ductal nos seguintes intervalos de tempo:zerohora(controle),24 horas,7,15,21,30 e 60 dias.Procedeu-se a an?lise do perfil imuno-histoqu?mico,onde verificou-se a express?o da calponina atrav?s de seu padr?o de distribui??o e ?ndice num?rico.Todos os esp?cimes exibiram positividade para a calponina nas c?lulas mioepiteliais,as quais se distribu?ram em torno dos ?cidos e estruturas ductais,sendo detectadas poucas c?lulas marcadas positivamente no grupo controle e no de 24 horas quando comparados aos grupos subsequentes,onde percebeu-se um grande aumento no n?mero de c?lulas marcadas positivamente,principlamente circundando as estruturas ductifformes que surgiram com o decorrer do tempo de obstru??o.Aplicados testes estat?sticos,foi verificado que o aumento no n?mero de c?lulas mioepiteliais positivas para a calponina,quando comparados o grupo controle(zero hora) com os grupos de 7,15,21,30 e 60 dias ap?s obstru??o,foi estatisticamente significativo.Concluiu-se que este aumento detectado,provavelmente ocorreu devido a uma eleva??o na taxa de prolifera??o das c?lulas mioepiteliais subsequente ? obstru??o ductal,associado a uma maior resist?ncia destas c?lulas ? arofia glandular.