No description available.

No description available.

This document explores the performance of taboo on the stage. The exploration is focused around the establishment of my personal aesthetic, which was developed through my studies at Virginia Commonwealth University. Starting with my first trip to Broadway, my journey goes through the class work that I did at VCU and the two plays that I did on the Shafer Street Alliance Laboratory Theatre stage: my performance and use of latex costumes in Funnyhouse of a Negro, and my direction and the troubles with producing Killer Joe. The exploration concludes with a look at the theatre in parts of Virginia and the ability to pursue an acting career with an aesthetic that pushes boundaries.

High-value industrial oils are essential for many processes and have great economic and environmental impacts. The tung tree produces a high-value seed oil. Approximately 80% of tung oil is α-eleostearic acid, which has a high degree of unsaturation thus giving it properties as a drying oil. The identification of the biological components in tung is imperative to further the knowledge of its processes. Four unique tung acyl-CoA binding proteins, VfACBP3a, VfACBP3b, VfACBP4, and VfACBP6 were identified and the genes encoding them were cloned and analyzed to determine their biological roles. The VfACBPs were observed to be similar to other organisms' ACBPs, especially Arabidopsis thaliana. In addition, each gene was expressed in all tung tissues. They were shown to interact with VfDGAT1 and VfDGAT2, two known components of tung lipid metabolism. Finally, VfACBP3a and VfACBP6 were expressed in the seeds of transgenic plants to study the effects of VfACBP expression on seed lipid fatty acid content.

In 1905 Albert Einstein developed the theory of Special Relativity. This theory describes the relation between space and time and revolutionized the understanding of the universe. While the concept is generally accepted new experimental setups are constantly being developed to challenge the theory, but so far no contradictions have been found. One of the postulates Einsteins theory of Relativity is based on states that the speed of light in vacuum is the highest possible velocity. Furthermore, it is demanded that the speed of light is independent of any chosen frame of reference. If an experiment would find a contradiction of these demands, the theory as such would have to be revised. To challenge the constancy of the speed of light the socalled Kennedy Thorndike experiment has been developed. A possible setup to conduct a Kennedy Thorndike experiment consists of comparing two independent clocks. Likewise experiments have been executed in laboratory environments. Within the scope of this work, the orbital requirements for the first space-based Kennedy Thorndike experiment called BOOST will be investigated.BOOST consists of an iodine clock, which serves as a time reference, and an optical cavity, which serves as a length reference. The mechanisms of the two clocks are different and can therefore be employed to investigate possible deviations in the speed of light. While similar experiments have been performed on Earth, space offers many advantages for the setup. First, one orbit takes roughly 90 min for a satellite based experiment. In comparison with the 24 h duration on Earth it is obvious that a space-based experiment offers higher statistics. Additionally the optical clock stability has to be kept for shorter periods, increasing the sensitivity. Third, the velocity of the experimental setup is larger. This results in an increased experiment accuracy since any deviation in the speed of light would increase with increasing orbital velocity. A satellite planted in a Low Earth Orbit (LEO) travels with a velocity of roughly 7 km/s. Establishing an Earth-bound experiment that travels with a constant velocity of that order is impossible. Finally, space offers a very quiet environment where no disturbances, such as vibrations, act upon the experiment, which is practically unavoidable in a laboratory environment. This thesis includes two main chapters. The chapter titled "Mission Level" exploits orbital candidates. Here, possible orbits are explained in detail and the associated advantages and problems are investigated. It also contains a discussion about ground visibility and downlink feasibility for each option. Finally, a nominal mission scenario is sketched. The other chapter is called "Sub-Systems". Within this chapter the subsystems of the spacecraft are examined. To examine the possible orbits it is necessary to define criteria according to which the quality of the orbits can be determined. The first criterion reflects upon the scientific outcome of the mission. This is mainly governed by the achievable velocity and the orbital geometry. The second criterion discriminates according to the mission costs. These include the launch, orbital injection, de-orbiting, satellite development, and orbital maintenance. The final criteria defines the requirements in terms of mission feasibility and risks, e.g. radiation. The criteria definition is followed by explaining the mission objectives and requirements. Each requirement is then discussed in terms of feasibility. The most important parameters, such as altitude, inclination, and the right ascension of the ascending node (RAAN), are discussed for each orbital option and an optimal range is picked. The optimal altitude depends on several factors, such as the decay rate, radiation concerns, experimental contributions, and eclipse duration. For the presented mission an altitude of 600 km seems to be the best fit. Alongside the optimal altitude possible de-orbiting scenarios are investigated. It is concluded that de-orbiting of the satellite is possible without any further external influence. Thus, no additional thrusters are required to de-orbit the satellite. The de-orbiting scenario has been simulated with systems tool kit (STK). From the simulation it can be concluded, that the satellite can be deorbited within 25 years. This estimation meets the requirements set for the mission. Another very important parameter is the accumulative eclipse duration per year for a given orbit. For this calculation it is necessary to know the relative positions and motion of the Earth and the Sun. From this the eclipse duration per orbit for different altitudes is gained. Ground visibilities for orbital options are examined for two possible ground stations. The theory is based on the geometrical relation between the satellite and the ground stations. The results are in an agreement with the related STK simulations. Finally, both ground stations are found adequate to maintain the necessary contact between the satellite and the ground station. In the trade-off section, orbit candidates are examined in more detail. Results from the previous sections with some additional issues such as the experiment sensitivities, radiation concern and thermal stability are discussed to conclude which candidate is the best for the mission. As a result of the trade-off, two scenarios are explained in the "Nominal Mission Scenario" section which covers a baseline scenario and a secondary scenario. After selecting a baseline orbit, two sub-systems of the satellite are examined. In the section of "Attitude Control System (ACS)" where the question of "Which attitude control method is more suitable for the mission?" is tried to be answered. A trade-off among two common control methods those are 3-axis stabilization and spin stabilization is made. For making the trade-off possible external disturbances in space are estimated for two imaginary satellite bodies. Then, it is concluded that by a spin stabilization method maintaining the attitude is not feasible. Thus, the ACS should be built on the method of 3-axis stabilization. As the second sub-system the possible power system of the satellite is examined. The total size and the weight of the solar arrays are estimated for two different power loads. Then, the battery capacity which will be sufficient for the power system budget is estimated together with the total mass of the batteries. In the last section, a conclusion of the thesis work is made and the possible future works for the BOOST mission are stated.

In what is historically a brief number of years, the life and times of John F. Kennedy have taken on legendary proportions. His presidency began with something less than a mandate from the American people, but he brought to the White House an inspiration and a style that offered great promises of things to come.

During the 1960's Guatemalan stability began to falter due to a political and social breakdown; guerilla violence and government repression emerged from this decade as common occurrences. In response to the instability within Guatemala, the US focused on providing significant financial aid to bolster a weak economy, while simultaneously working with the Guatemalan police and military to create more efficient and modern internal security forces capable of combating Communist subversion. Despite US attempts to foster stability, in 1963 President Miguel Ydigoras Fuentes was removed from office by a military coup organized by his opponents within Guatemala. The Lyndon B. Johnson administration continued to support the Guatemalan government and continued to provide economic and military assistance. Despite US assistance, the internal social and political divisions in Guatemala continued to result in violence. In the midst of the escalating violence, elections were held in 1966 and the center left candidate Julio Cesar Mendez Montenegro was elected as the new president of Guatemala. The election of a politically left president further radicalized the Guatemalan right, which resulted in attempted coups and acts of terror. The violence from the leftist guerillas and the radical rightist elements forced Mendez Montenegro to allow the military to use harsh counter-terror strategies to bring the country under control. Despite negative developments, the US consistently tried to help build Guatemalan stability. Unfortunately, its policies ignored the socio-economic inequalities, and internal division which was the biggest problem facing the nation. The internal political division that created the violence and instability made it impossible for any US assistance to have a meaningful impact. During the 1960's these developments in Guatemala paved the way for the violence and genocide of the 1980's and solidified a policy of US involvement that was inadequate and ineffective.

Soybean (Glycine max (L.) Merr) is the largest oil and protein crop in the world and it is grown for both oil and protein. To address the needs of both the edible oil market and industrial applications of soybean oil, fatty acid modification has been a focus of soybean breeding programs. Natural variation, mutagenesis and genetic engineering have been used to alter the fatty acid profile. Several genes, mostly desaturases, have been associated with altered fatty acid profiles but enzymes in the Kennedy Pathway have yet to be studied as another source of genetic variation for altering the fatty acid profiles. The Kennedy Pathway is also known as the oil producing pathway and consists of four enzymes: glycerol-3-phosphate acyltransferase (G3PAT); lysophosphatidic acid acyltransferase (LPAAT); phosphatidic acid phosphatase (PAP); and diacylglycerol acyltransferase 1 (DGAT1). The starting material for this pathway is glycerol-3-phosphate, which is produced from glycerol by glycerol kinase (GK), and the product of this pathway is triacylglycerol (TAG). The overall objective of this study was to elucidate the role that the Kennedy Pathway plays in determining the fatty acid profile in two ways: (1) sequencing the transcribed region of the genomic genes encoding the enzymes of GK, G3PAT, LPAAT, and DGAT1 in soybean genotypes with altered fatty acid profiles; and (2) studying their expression over seed development, across three growing temperatures. The genetic material for the study consisted of four soybean genotypes with altered fatty acid profile: RG2, RG7, RG10, and SV64-53. Results from sequencing showed that the mutations identified in G3PAT, LPAAT, and DGAT1 in the four soybean genotypes did not explain the differences in the fatty acid profiles. The expression of G3PAT, LPAAT, and DGAT1 over seed development showed that G3PAT had the lowest levels, followed by LPAAT, then DGAT1, across the growing temperatures. The differences in expression among genotypes corresponded to differences in fatty acid accumulation, suggesting that expression rather than genetic mutations in the transcribed region of the genes influenced the fatty acid profile of the genotypes in this study. In conclusion, the enzymes of the Kennedy Pathway appear to contribute to the altered fatty acid profiles observed in the soybean mutant genotypes. / Ontario Ministry of Economic Development and Innovation (formerly Ontario Ministry of Research and Innovation), BioCar Initiative, Grain Farmers of Ontario, SeCan

No description available.

The human genome project has allowed for the rapid identification of a large number of protein families based on similarities in their genetic sequences. The acyl-glycerol phosphate acyltransferase (AGPAT) family of enzymes have been largely identified through sequence homology, with eleven isoforms identified in both mice and humans. Interestingly, very little work has been done on the characterization of AGPAT isoform 4. In the present study, I report the functional characterization of AGPAT4 as an acyl-CoA: lysocardiolipin acyltransferase (ALCAT), which we have renamed ALCAT2. Although ALCAT2 is present in most tissues, it is abundant in multiple brain regions including olfactory bulbs, hippocampus, cerebellum, cortex, and brain stem, and is detectable in both primary neurons and glial cells. In assays performed in vitro, ALCAT2 significantly increased the incorporation of [14C]oleoyl-CoA into phosphatidylinositol and CL using either lysophosphatidylinositol, or monolysocardiolipin or dilysocardiolipin as acyl acceptors, respectively. ALCAT2 did not display significant acyltransferase activity with lysophosphatidic acid, lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylserine, or lysophosphatidylglycerol acyl acceptors. Overexpressing ALCAT2 in HEK-293 cells increased the total CL content, but did not significantly affect levels of other glycerophospholipids including phosphatidylinositol. Analysis of the fatty acyl profile of CL from ALCAT2-overexpressing cells indicated increased total saturated fatty acids, particularly stearate, palmitate, and myristate, and increased levels of n-3 polyunsaturated fatty acids α-linolenic acid (18:3n-3), eicosatrienoic acid (20:3n-3), and eicosapentanoic acid (20:5n-3). In accordance with its observed role in cardiolipin remodeling, ALCAT2 localized predominately to the mitochondria. ALCAT2 was also regulated during embryogenesis, and in varying metabolic states. In summary, ALCAT2 is a new enzyme in CL remodeling with a potential role in mitochondrial function.