The term “at risk” is a label that is used to describe students who encompass a host of prominent socially and politically constructed titles that are intended to simplify student understanding and awareness and allow for clear reporting. The purpose of this study was to demythologize the concept of “at risk” by creating the conditions for student voice and critical dialogue to emerge, through the use of narrative inquiry. This research hoped to provide an outlet for young people to find and use their own voices, while finding their own place within their lived histories. The research also aimed to raise awareness of the reality of the contemporary U.S. educational system: we often create policies and programs without considering the perspectives of the young people whom these services are designed to serve. Through critical narrative inquiry, six former student’s engaged in unstructured interviews and a focus group. Through analysis of the data set, five themes emerged and include 1) relationships with bicultural adults who understand, 2) instrumentalizing pedagogy, 3) the impact of money-driven policy, 4) the awareness of limitations of opportunities, and 5) the overall theme of the transparency of hegemony. This research hoped to problematize the label in an effort to move toward an emancipatory understanding of how we speak about young people and make sense of the circumstances these young people must navigate through their education and their world.

No

A new, standardized food label developed by the Food and Drug Administration is appearing on products this year. Extensive research on consumer use suggests that approximately 20 % of the U.S. population, composed mainly of elderly and minorities, cannot correctly interpret the nutrition information on the label. This research explored the specific knowledge required for correct interpretation based on a model in which nutrition knowledge was organized in hierarchical levels: food groups, macronutrients and micronutrients in foods, with each level including dimensions of nutrition terminology, health relationships and related mathematics skills. An instrument was developed and pilot tested to measure knowledge at each level, as well as to measure accuracy in food label interpretation. Test items were revised based on peer input, correlational data, item analysis, and reliability. The revised instrument was then administered to purposive samples of adults (250 subjects) representing the range of nutrition knowledge measured by the test. Scores were re-analyzed to establish the validity and reliability of the new instrument. Factor analysis was used to explore the value of the original hierarchical model and to posit an additional model based on conceptual complexity. Hierarchical multiple regression was used to predict accuracy of food label interpretation based on factors depicted by both models. Findings indicated that the structures outlined in each model are useful predictors of food label interpretation, accounting for over 52 percent of the variance. Suggestions are made for further development of the test instrument and on how to incorporate learner pre-assessment in designing nutrition education interventions. / Ed. D.

The purpose of this study was to qualitatively examine the meaning that psychotherapeutic patients associate with their self-esteem in order to contribute to a better understanding of what these patients' views of low and high self-esteem entail. A study of literature was conducted to expose different aspects of the term 'self-esteem' from several theoretical approaches. Furthermore, the substructure of the hypno-therapeutic approach (of which the medical hypno-analytical model was the main approach) was especially analysed to ground the psychotherapeutic treatment received by the research participants. Eleven research participants were involved in the study according to the extreme, as well as the confirming and disconfirming case methods of sampling. They were selected on the basis of homogenous characteristics, namely: white, Afrikaans-speaking men in the low to high average socio-economic sphere. This study was done within a qualitative or interpretive paradigm - the study therefore focuses on the understanding (verstehen) of the meaning individuals attach to their self-esteem and not on the explanation (erklärung) thereof. The data gathering techniques were the basic individual interview, the self-esteem induction and observation. The textual data, transcribed from audiocassettes, was analysed qualitatively with the help of the ATLAS.ti-program according to the principles and procedures of grounded theory (including open, axial and selective coding). Data analysis led to, amongst other things, the co-construction of 23 categories (with numerous subcategories) of which the central phenomenon was identified as 'low self-esteem'. Relations could be drawn between the core category and other categories and the following storyline, which could suggest a possible theory, was conceptualised: -Negative suggesting in the patient's past leads to low self-esteem, which is unhealthily handled within his emotional problematics by means of a negative thinking scheme for which therapy is received from a medical hypno-analytical perspective to treat the negative labels in order to facilitate attachment of positive meaning to his self-esteem. The meaning(s) ascribed by psychotherapeutic patients to their self-esteem seem to be, in accordance with the post-modern spirit of the age (Zeitgeist), holistic in nature. / Die doelstelling van hierdie studie was om die betekenis wat psigoterapeutiese pasiente met hul selfagting assosieer, kwalitatief te ondersoek ten einde 'n bydrae te maak tot 'n beter begrip van wat psigoterapeutiese pasiente se siening van lae en hoe selfagting behels. 'n Literatuurstudie is uitgevoer om die onderskeie aspekte met betrekking tot die begrip 'selfagting' vanuit verskillende teoretiese benaderings te belig. Verder is die onderbou van veral die hipnoterapeutiese benadering (waarvan die mediese hipnoanalitiese model die hoofbenadering was) ge-analiseer om die psigoterapeutiese behandeling wat die navorsingsdeelnemers ontvang het, te fundeer. Elf navorsingsdeelnemers is volgens die ekstreme, sowel as die bevestigende en niebevestigende gevallemetodes van steekproeftrekking, by die studie betrek. Hulle is op grond van homogene eienskappe gekies, naamlik: blanke, Afrikaanssprekende mans binne 'n laag tot hoog gemiddelde sosio-ekonomiese sfeer. Hierdie studie is binne 'n kwalitatiewe of interpretatiewe paradigma gedoen - die studie fokus dus op die verstaan (verstehen) van die betekenis wat individue aan hul selfagting heg en nie op die verklaring (erklarung) daarvan nie. Die data- insamelingstegnieke was die basiese individuele onderhoud, die selfagtinginduksie in hipnoterapie en observasie. Die data is as tekstuele data, wat vanaf klankkassette getranskribeer is, op kwalitatiewe wyse met behulp van die ATLASJi-program aan die hand van die beginsels en prosedures van gegronde teorie (waaronder oop, aksiale en selektiewe kodering) ontleed. Data-ontleding het onder andere gelei tot die ko-konstruksie van 23 kategoriee (met talle subkategoriee) waarvan die sentrale verskynsel as 'lae selfagting' geidentifiseer is. Verbande kon tussen die kernkategorie en ander kategoriee getrek word, en die volgende gekonseptualiseerde storielyn wat op 'n moontlike teorie kan dui, is gekokonstrueer: - Negatiewe suggerering in die pasient se verlede lei tot sy lae selfagting wat hy binne sy emosionele problematiek deur middel van 'n negatiewe denkskema ongesond hanteer en dan psigoterapie ontvang vir die behandeling van sy negatiewe etikette sodat 'n positiewe betekenisgewing aan sy selfagting vanuit 'n mediese hipno-analitiese perspektief gefasiliteer kan word. Die betekenis(se) wat die psigoterapeutiese pasiente aan hul selfagting gee, blyk, in ooreenstemming met die postmodernistiese tydgees, holisties van aard te wees. / Psychology / D. Litt. et Phil. (Psychology)

Le dioxyde de carbone (CO2) est un résidu naturel du métabolisme cellulaire, la troisième substance la plus abondante du sang, et un important agent vasoactif. À la moindre variation de la teneur en CO2 du sang, la résistance du système vasculaire cérébral et la perfusion tissulaire cérébrale subissent des changements globaux. Bien que les mécanismes exacts qui sous-tendent cet effet restent à être élucidés, le phénomène a été largement exploité dans les études de réactivité vasculaire cérébrale (RVC). Une voie prometteuse pour l’évaluation de la fonction vasculaire cérébrale est la cartographie de la RVC de manière non-invasive grâce à l’utilisation de l’Imagerie par Résonance Magnétique fonctionnelle (IRMf). Des mesures quantitatives et non-invasives de de la RVC peuvent être obtenus avec l’utilisation de différentes techniques telles que la manipu- lation du contenu artériel en CO2 (PaCO2) combinée à la technique de marquage de spin artériel (Arterial Spin Labeling, ASL), qui permet de mesurer les changements de la perfusion cérébrale provoqués par les stimuli vasculaires. Toutefois, les préoccupations liées à la sensibilité et la fiabilité des mesures de la RVC limitent de nos jours l’adoption plus large de ces méthodes modernes de IRMf. J’ai considéré qu’une analyse approfondie ainsi que l’amélioration des méthodes disponibles pourraient apporter une contribution précieuse dans le domaine du génie biomédical, de même qu’aider à faire progresser le développement de nouveaux outils d’imagerie de diagnostique. Dans cette thèse je présente une série d’études où j’examine l’impact des méthodes alternatives de stimulation/imagerie vasculaire sur les mesures de la RVC et les moyens d’améliorer la sensibilité et la fiabilité de telles méthodes. J’ai aussi inclus dans cette thèse un manuscrit théorique où j’examine la possible contribution d’un facteur méconnu dans le phénomène de la RVC : les variations de la pression osmotique du sang induites par les produits de la dissolution du CO2. Outre l’introduction générale (Chapitre 1) et les conclusions (Chapitre 6), cette thèse comporte 4 autres chapitres, au long des quels cinq différentes études sont présentées sous forme d’articles scientifiques qui ont été acceptés à des fins de publication dans différentes revues scientifiques. Chaque chapitre débute par sa propre introduction, qui consiste en une description plus détaillée du contexte motivant le(s) manuscrit(s) associé(s) et un bref résumé des résultats transmis. Un compte rendu détaillé des méthodes et des résultats peut être trouvé dans le(s) dit(s) manuscrit(s). Dans l’étude qui compose le Chapitre 2, je compare la sensibilité des deux techniques ASL de pointe et je démontre que la dernière implémentation de l’ASL continue, la pCASL, offre des mesures plus robustes de la RVC en comparaison à d’autres méthodes pulsés plus âgées. Dans le Chapitre 3, je compare les mesures de la RVC obtenues par pCASL avec l’utilisation de quatre méthodes respiratoires différentes pour manipuler le CO2 artérielle (PaCO2) et je démontre que les résultats peuvent varier de manière significative lorsque les manipulations ne sont pas conçues pour fonctionner dans l’intervalle linéaire de la courbe dose-réponse du CO2. Le Chapitre 4 comprend deux études complémentaires visant à déterminer le niveau de reproductibilité qui peut être obtenu en utilisant des méthodes plus récentes pour la mesure de la RVC. La première étude a abouti à la mise au point technique d’un appareil qui permet des manipulations respiratoires du CO2 de manière simple, sécuritaire et robuste. La méthode respiratoire améliorée a été utilisée dans la seconde étude – de neuro-imagerie – où la sensibilité et la reproductibilité de la RVC, mesurée par pCASL, ont été examinées. La technique d’imagerie pCASL a pu détecter des réponses de perfusion induites par la variation du CO2 dans environ 90% du cortex cérébral humain et la reproductibilité de ces mesures était comparable à celle d’autres mesures hémodynamiques déjà adoptées dans la pratique clinique. Enfin, dans le Chapitre 5, je présente un modèle mathématique qui décrit la RVC en termes de changements du PaCO2 liés à l’osmolarité du sang. Les réponses prédites par ce modèle correspondent étroitement aux changements hémodynamiques mesurés avec pCASL ; suggérant une contribution supplémentaire à la réactivité du système vasculaire cérébral en lien avec le CO2. / Carbon dioxide (CO2) is a natural byproduct of cellular metabolism, the third most abundant substance of blood, and a potent vasoactive agent. The resistance of cerebral vasculature and perfusion of the brain tissue respond to the slightest change in blood CO2 content. The physiology of such an effect remains elusive, yet the phenomenon has been widely exploited in studies of the cerebral vascular function. A promising avenue for the assessment of brain’s vascular function is to measure the cerebrovascular reactivity to CO2 (CVR) non-invasively using functional MRI. Quantitative and non-invasive mapping of CVR can be obtained using respiratory manipulations in arterial CO2 and Arterial Spin Labeling (ASL) to measure the perfusion changes associated with the vascular stimulus. However, concerns related to the sensitivity and reliability of CVR mea- sures by ASL still limit their broader adoption. I considered that a thorough analysis and amelioration of available methods could bring a valuable contribution in the domain of biomedical engineering, helping to advance new diagnostic imaging tools. In this thesis I present a series of studies where I exam the impact of alternative manipulation/ASL methods on CVR measures, and ways to improve the sensitivity and reliability of these measures. I have also included in this thesis a theoretical paper, where I exam the possible contribution of an unappreciated factor in the CVR phenomenon: the changes in blood osmotic pressure induced by the products of CO2 dissolution. Apart from a general introduction (Chapter 1) and conclusion (Chapter 6), this thesis comprises 4 other chapters, in which five different research studies are presented in the form of articles accepted for publication in scientific journals. Each of these chapters begins with its own specific introduction, which consists of a description of the background motivating the study and a brief summary of conveyed findings. A detailed account of methods and results can be found in the accompanying manuscript(s). The study composing Chapter 2 compares the sensitivity of two state-of-the-art ASL techniques and show that a recent implementation of continuous ASL, pCASL, affords more robust measures of CVR than older pulsed methods. The study described in Chapter 3 compares pCASL CVR measures obtained using 4 different respiratory methods to manipulate arterial CO2 (PaCO2) and shows that results can differ significantly when manipulations are not designed to operate at the linear range of the CO2 dose-response curve. Chapter 4 encompasses two complementary studies seeking to determine the degree of reproducibility that can be attained measuring CVR using the most recent methods. The first study resulted in the technical development of a breathing apparatus allowing simple, safe and robust respiratory CO2 manipulations. The improved respiratory method was used in the second – neuroimaging – study, in which I and co-authors investigate the sensitivity and reproducibility of pCASL measuring CVR. The pCASL imaging technique was able to detect CO2-induced perfusion responses in about 90% of the human brain cortex and the reproducibility of its measures was comparable to other hemodynamic measures already adopted in the clinical practice. Finally, in Chapter 5 I present a mathematical model that describes CVR in terms of PaCO2-related changes in blood osmolarity. The responses predicted by this model correspond closely to the hemodynamic changes measured with pCASL, suggesting an additional contribution to the reactivity of cerebral vasculature to CO2.

A ingestão de ácidos graxos trans tem sido consistentemente mostrada ter efeitos adversos nos lipídeos sanguíneos, principalmente na razão LDL:HDL colesterol, que é um forte marcador de risco cardiovascular. De acordo com a Resolução RDC (Resolução da Diretoria Colegiada) de número 360 da ANVISA, de 23 de dezembro de 2003 da ANVISA, (Agência Nacional de Vigilância Sanitária), após 1 de agosto de 2006 as indústrias de alimentos devem declarar o conteúdo de ácidos graxos trans por porção do produto. O objetivo deste trabalho foi analisar a composição de ácidos graxos trans em margarinas e cremes vegetais após a nova legislação. As margarinas e cremes vegetais foram adquiridos na cidade de São Paulo, perfazendo um total de 40 amostras, 17 margarinas cremosas, 8 margarinas light, 1 margarina culinária cremosa, 2 margarinas culinária duras, 1 margarina culinária líquida, 2 alimentos a base de margarina, 8 cremes vegetais e 1 creme vegetal light foram analisados. Os lipídeos foram extraídos por hidrólise ácida, derivatizados com BF3 e em seguida analisados em cromatógrafo gasoso, equipado com coluna capilar SP- 2560 de 100 m a 180C. Os teores de trans totais das margarinas interesterificadas sofreram aumento significativo no período compreendido entre os anos 2000 a 2006, variando de 0 2,17% e 0,71 2,32% respectivamente. Nas margarinas hidrogenadas também foi observado aumento de 11,56 20,55% para 12,63 26,00% em 2006. As margarinas culinárias duras foram o tipo de margarina que apresentou concentrações elevadas de trans, variando de 19,38 a 30,35%. A margarina culinária cremosa e a margarina culinária líquida continham baixos teores de trans, 1,62 e 3,32% respectivamente. Os cremes vegetais interesterificados não sofreram mudança significativa, passando de 0 1,70% para 0 1,66%. No creme vegetal hidrogenado ocorreu redução acentuada dos teores médios de trans totais de 20,55 para 12,63%. A mudança na legislação não foi suficiente para reduzir totalmente os teores de ácidos graxos trans nas margarinas e cremes vegetais. Apesar disto, foi observado um aumento da disponibilidade de margarinas e cremes vegetais zero trans, que são aqueles que contém teores ≤ 0,2 g/porção de 10 g. / Intake of trans fatty acids (TFA) has been consistently shown to have adverse effects on blood lipids, most notably on the LDL:HDL cholesterol ratio, which is a strong marker of cardiovascular risk. According to RDC (Resolução de Diretoria Colegiada) resolution number 360 of the ANVISA (Agência Nacional de Vigilância Sanitária National Agency for Sanitary Vigilance), after August 1, 2006, food industries must declare the trans fatty acid (TFA) content per product serving. The objective of this work was to analyzer the composition of trans fatty acid in margarines and fat spreads after the new legislation. The margarines and fat spread were obtained in the city of São Paulo, making a total of 40 samples, seventeen tub margarines, eight light tub margarines, one culinary tub margarine, two culinary hard margarines, one culinary liquid margarine, two margarine-based foods, eight fat spreads and one light fat spread were analyzed. The lipids were extracted by acid hydrolysis, derivatizados with BF3 and then analyzed by gas chromatograph, equipped with capillary column SP-2560 of 100 m a 180 C. The total TFA content in interesterified margarines significantly increased between 2000 and 2006, rising from 02.17% to 0.712.32%. For hydrogenated margarines, an increase was also observed, from 11.5620.55% to 12.6326.00% by 2006. The culinary hard margarines were the type of margarine that had high concentrations of trans, ranging from 19.38 to 30.35%. Culinary tub margarine and culinary liquid margarine contained low levels of trans, 1.62 and 3.32% respectively. Interesterified fat spreads did not significantly change, from 0 1.70% to 01.66%. In hydrogenated fat spreads, a sharp reduction was seen, with average levels of total trans fats falling from 20.55% to 12.63%. Changes in the legislative regulation were not sufficient to significantly reduce the levels of TFA in margarines and fat spreads. Nevertheless, an increased availability of zero trans fat margarines and fat spreads on the market was observed (levels ≤ 0.2 g per 10 g serving).

Die magnetische Blutbolusmarkierung (Spin-Labeling) ermöglicht die nichtinvasive quantitative Messung des Blutflusses im Gewebe. Beim Spin-Labeling wird arterielles Blut durch Radiofrequenzpulse magnetisch markiert und der Transport der Markierung MR-tomographisch gemessen. Am Modell einer unter physiologischen Bedingungen perfundierten extrakorporalen Schweineniere konnte die Quantifizierbarkeit der Messmethode nachgewiesen werden. In einer Studie an 36 Hirntumorpatienten wurde das Verfahren mit der kontrastmittelbasierten First-Pass-Bolus-Methode zur nicht-quantitativen Perfusionsmessung verglichen. Es zeigte sich eine sehr gute Übereinstimmung zwischen beiden Methoden, der lineare Korrelationskoeffizient des relativen Blutflusses in der Tumorregion lag bei R=0,83. Die mittels Spin-Labeling ermittelten Absolutwerte des Blutflusses spielen bei der Beurteilung des Tumorgrades eine untergeordnete Rolle, da die mittlere Perfusion individuell sehr verschieden ist. Ein zweiter Anwendungsbereich für das Spin-Labeling ist die Darstellung großer Arterien. Spin-Labeling ermöglicht die nichtinvasive dynamische Angiographie (Dynamische Spin-Labeling-Angiographie - DSLA). Analog zur digitalen Subtraktionsangiographie kann damit der Einstromvorgang des Blutes in den Gefäßbaum zeitaufgelöst gemessen werden, jedoch mit wesentlich höherer zeitlicher Auflösung und frei wählbarer Projektionsrichtung. In einer Studie an 18 Patienten mit einseitigen Carotisstenosen wurden die Zeitdifferenzen der Anflutung der zerebralen Gefäße zwischen der betroffenen und der nicht stenosierten Seite bestimmt. Die im Carotis-Siphon gemessenen Zeitdifferenzen korrelieren signifikant mit dem Stenosegrad, steigen aber erst ab einer Lumeneinengung oberhalb von 80 Prozent deutlich an. Im Vergleich zu den etablierten Methoden werden die Möglichkeiten und Grenzen der DSLA dargestellt. / Arterial spin labeling methods allow to determine quantitative tissue blood flow values noninvasively. Arterial blood is labelled by an inversion pulse and the distribution of this intrinsic tracer is measured using magnetic resonance imaging. Experiments using an extra corporal in-vitro porcine kidney in a MR compatible set-up were carried out to determine the accuracy of blood flow values calculated from arterial spin labeling measurements. In a study of 36 brain tumor patients, spin labeling was compared to non-quantitative contrast-enhanced dynamic susceptibility-weighted perfusion imaging. Relative blood flow values determined with both methods were in good agreement, the linear regression coefficient in the tumor region was R=0.83. Due to the variable individual perfusion state, quantitative blood flow values determined using spin labeling play a minor role in the assessment of tumor grade. Application of spin labeling to angiography of major arteries was investigated. Dynamic spin labeling angiography (DSLA) sequences were implemented and tested on a clinical scanner. This technique allows time-resolved depiction of blood flow in large vessels with very high temporal resolution. As opposed to digital subtraction angiography, the method allows arbitrary projection directions. In a study, 18 patients with one-sided carotid stenoses were examined. In these patients the time differences of blood bolus arrival at both hemispheres were determined. Time differences measured in the carotid siphon show a significant correlation with the degree of stenosis. However, a clear increase is not seen until 80% narrowing of a carotid. Possibilities and limitations of the DSLA method are discussed in comparison to established techniques.

by Lam Lai Ming, Regina, Tang Oi Tai. / Thesis (M.B.A.)--Chinese University of Hong Kong, 2002. / Includes bibliographical references (leaves 65-66). / Questionnaire also in Chinese. / ABSTRACT --- p.ii / TABLE OF CONTENTS --- p.iv / LIST OF TABLES --- p.vi / LIST OF FIGURES --- p.vii / ACKNOWLEDGEMENT --- p.ix / Chapter / Chapter I. --- INTRODUCTION --- p.1 / Genes and GM Food --- p.1 / Examples of GM Food Available in the Market --- p.2 / Potential Benefits and Risks of GM Food --- p.2 / Scientific Evidence for the Safety of GM Food --- p.5 / The International Scene of GM Food Labeling System --- p.6 / Pros and Cons of GM Food Labeling --- p.7 / Consumers' Perception and Acceptance of GM Food --- p.9 / Situation in Hong Kong --- p.11 / Hong Kong Public´ةs Concern --- p.13 / Chapter II. --- RESEARCH OBJECTIVES AND METHODOLOGY --- p.15 / Research Objectives --- p.15 / Methodology --- p.15 / Research Design --- p.15 / Questionnaire --- p.16 / Data Collection --- p.17 / Data Analysis Method --- p.17 / Chapter III. --- FINDINGS AND IMPLICATIONS --- p.19 / General Public's knowledge about / awareness of GM food --- p.19 / Consumers' Perception and Attitudes towards GM Food and Traditionally Produced Food --- p.22 / Quality --- p.25 / Nutritional Value --- p.27 / Price --- p.29 / Safety of consumption --- p.31 / Consumers' Purchasing Behavior --- p.34 / To evaluate consumers' opinions and reactions to the GM Food labeling system in Hong Kong --- p.40 / Profiles of the respondents --- p.43 / Limitations --- p.46 / Chapter IV. --- RECOMMENDATIONS --- p.48 / For Government --- p.48 / For GM Food Manufacturers --- p.50 / For Suppliers --- p.51 / APPENDIX --- p.53 / BIBLIOGRAPHY --- p.65

This dissertation describes the advantages and feasibility of newly synthesized near-infrared (NIR) bis-heptamethine cyanine (BHmC) dyes for non-covalent labeling schemes. The NIR BHmCs were synthesized for biomolecule assay. The advantages of NIR BHmCs for biomolecule labeling and the instrumental advantages of the near-infrared region are also demonstrated. Chapter 1 introduces the theory and applications of dye chemistry. For bioanalysis, this chapter presents covalent and non-covalent labeling. The covalent labeling depends on the functionality of amino acids and the non-covalent labeling relies on the binding site of a protein. Due to the complicated binding process in non-covalent labeling, this chapter also discusses the binding equilibria in spectroscopic and chromatographic analyses. Chapter 2 and 3 evaluate the novel BHmCs for non-covalent labeling with human serum albumin (HSA) and report the influence of micro-environment on BHmCs. The interesting character of BHmCs in aqueous solutions is that the dyes exhibit non- or low-fluorescence compared to their monomer counterpart, RK780. It is due to their H-type closed clam-shell form in the solutions. The addition of HSA or organic solvents opens up the clam-shell form and enhances fluorescence. The binding equilibria are also examed. Chapter 4 provides a brief introduction that summaries the use of capillary electrophoresis (CE), and offers a detailed instrumentation that discusses the importance and advantage of a detector in NIR region for CE separation. Chapter 5 focuses on the use of NIR cyanine dyes with capillary electrcophoresis with near-infrared laser induce fluorescence (CE-NIR-LIF) detection. The NIR dyes with different functional groups show that RK780 is a suitable NIR dye for HSA labeling. The use of BHmCs with CE-NIR-LIF reduces signal noises that are commonly caused by the interaction between NIR cyanine dyes and negatively charged capillary wall. In addition, bovine carbonic anhydrase II (BCA II) is applied to study the influence of hydrophobicity on non-covalent labeling. Finally, chapter 6 presents the conformational dependency of BHmCs on the mobility in capillary and evaluates the further possibility of BHmCs for small molecule detection. Acridine orange (AO) is used as a sample and it breaks up the aggregate and enhances fluorescence. The inserted AO into BHmC changes the mobility in capillary, owing to the conformational changes by AO.

The purpose of this study was to qualitatively examine the meaning that psychotherapeutic patients associate with their self-esteem in order to contribute to a better understanding of what these patients' views of low and high self-esteem entail. A study of literature was conducted to expose different aspects of the term 'self-esteem' from several theoretical approaches. Furthermore, the substructure of the hypno-therapeutic approach (of which the medical hypno-analytical model was the main approach) was especially analysed to ground the psychotherapeutic treatment received by the research participants. Eleven research participants were involved in the study according to the extreme, as well as the confirming and disconfirming case methods of sampling. They were selected on the basis of homogenous characteristics, namely: white, Afrikaans-speaking men in the low to high average socio-economic sphere. This study was done within a qualitative or interpretive paradigm - the study therefore focuses on the understanding (verstehen) of the meaning individuals attach to their self-esteem and not on the explanation (erklärung) thereof. The data gathering techniques were the basic individual interview, the self-esteem induction and observation. The textual data, transcribed from audiocassettes, was analysed qualitatively with the help of the ATLAS.ti-program according to the principles and procedures of grounded theory (including open, axial and selective coding). Data analysis led to, amongst other things, the co-construction of 23 categories (with numerous subcategories) of which the central phenomenon was identified as 'low self-esteem'. Relations could be drawn between the core category and other categories and the following storyline, which could suggest a possible theory, was conceptualised: -Negative suggesting in the patient's past leads to low self-esteem, which is unhealthily handled within his emotional problematics by means of a negative thinking scheme for which therapy is received from a medical hypno-analytical perspective to treat the negative labels in order to facilitate attachment of positive meaning to his self-esteem. The meaning(s) ascribed by psychotherapeutic patients to their self-esteem seem to be, in accordance with the post-modern spirit of the age (Zeitgeist), holistic in nature. / Die doelstelling van hierdie studie was om die betekenis wat psigoterapeutiese pasiente met hul selfagting assosieer, kwalitatief te ondersoek ten einde 'n bydrae te maak tot 'n beter begrip van wat psigoterapeutiese pasiente se siening van lae en hoe selfagting behels. 'n Literatuurstudie is uitgevoer om die onderskeie aspekte met betrekking tot die begrip 'selfagting' vanuit verskillende teoretiese benaderings te belig. Verder is die onderbou van veral die hipnoterapeutiese benadering (waarvan die mediese hipnoanalitiese model die hoofbenadering was) ge-analiseer om die psigoterapeutiese behandeling wat die navorsingsdeelnemers ontvang het, te fundeer. Elf navorsingsdeelnemers is volgens die ekstreme, sowel as die bevestigende en niebevestigende gevallemetodes van steekproeftrekking, by die studie betrek. Hulle is op grond van homogene eienskappe gekies, naamlik: blanke, Afrikaanssprekende mans binne 'n laag tot hoog gemiddelde sosio-ekonomiese sfeer. Hierdie studie is binne 'n kwalitatiewe of interpretatiewe paradigma gedoen - die studie fokus dus op die verstaan (verstehen) van die betekenis wat individue aan hul selfagting heg en nie op die verklaring (erklarung) daarvan nie. Die data- insamelingstegnieke was die basiese individuele onderhoud, die selfagtinginduksie in hipnoterapie en observasie. Die data is as tekstuele data, wat vanaf klankkassette getranskribeer is, op kwalitatiewe wyse met behulp van die ATLASJi-program aan die hand van die beginsels en prosedures van gegronde teorie (waaronder oop, aksiale en selektiewe kodering) ontleed. Data-ontleding het onder andere gelei tot die ko-konstruksie van 23 kategoriee (met talle subkategoriee) waarvan die sentrale verskynsel as 'lae selfagting' geidentifiseer is. Verbande kon tussen die kernkategorie en ander kategoriee getrek word, en die volgende gekonseptualiseerde storielyn wat op 'n moontlike teorie kan dui, is gekokonstrueer: - Negatiewe suggerering in die pasient se verlede lei tot sy lae selfagting wat hy binne sy emosionele problematiek deur middel van 'n negatiewe denkskema ongesond hanteer en dan psigoterapie ontvang vir die behandeling van sy negatiewe etikette sodat 'n positiewe betekenisgewing aan sy selfagting vanuit 'n mediese hipno-analitiese perspektief gefasiliteer kan word. Die betekenis(se) wat die psigoterapeutiese pasiente aan hul selfagting gee, blyk, in ooreenstemming met die postmodernistiese tydgees, holisties van aard te wees. / Psychology / D. Litt. et Phil. (Psychology)