Background: The agro-food sector faces several sustainability challenges and is in clear need of transitions to become more sustainable. A transition in food production, consumption and supply chains that demand fewer resources and fewer animal products is required to attain sustainability in the agro-food sector. This transition can be done by consuming more plant-based food and providing an alternate option to animal protein by substituting it with plant protein. Purpose: This study aims to understand the technological innovation system shaping around the value chain of Swedish legumes. To investigate the above aim, this research intends to address the following questions: RQ1: How is the innovation system built up, and how do the functions of the innovation system perform around the value chain of Swedish legumes? RQ2: What are the barriers connected to the innovation system that hinders the development of the value chain of Swedish legumes? Method: The methodology used was a qualitative case study with an abductive approach. The data was collected from 8 semi-structured interviews and a literature review of relevant articles. The empirical findings were then analysed using a framework based on Technology Innovation System (TIS). Implications: This research study describes the structural causes that form functional barriers in the innovation system that hinders the development of a value chain for Swedish legumes. The findings of this study provides an excellent opportunity for government agencies and policymakers to guide plans and actions through policies to provide incentives for farmers and research projects in favour of a sustainable transition towards more plant-based food. Also, our study contributes to an understanding of sustainability transitions in the Swedish agro-food sector by analysing how the TIS functions perform around the value chain of Swedish legumes.

MSCAGR (Soil Science) / Department of Soil Science / Maize (Zea mays L.) is widely grown in the semi-arid regions of South Africa mainly for its grain that is used for direct human consumption, feed for animals and raw materials for the industries. The challenges of soil infertility, water supply, and availability of high yielding cultivars remain a major constraint for its production in this environment. These constraints are a major threat to sustainable crop production and food security. Maize/lablab Zea mays L.\ L. purpureus) intercropping system could thus become an option for food security among small scale maize producers in dry environments. Preliminary studies show the huge potential of maize/lablab intercropping in the semi-arid environments of the North-Eastern South Africa. Therefore, this study aimed to assess the effects of maize/lablab intercropping on soil water content, nitrogen dynamics and crop productivity based field experiments and crop simulation modeling using the model APSIM. The trials were conducted at two sites (Univen and Syferkuil) in Limpopo province, South Africa, for two seasons (2015/2016) and 2016/2017). The treatments consisted of; (i) sole maize (ii) sole lablab (iii) maize and lablab planted at the same time (Maize+lablab-ST) and (iv) maize with lablab planted 28 days after maize (Maize+lablab-28).The treatments were laid out in an RCBD replicated 4 times, with individual plots size measuring 4.5 m × 4 m (18 m2) and the layout of the field as consisting of 4 plots per block giving a total of 16 plots in 4 blocks. The following parameters were determined: soil water content, soil NO3--N and NH4+-N levels, dry matter and grain yield. The APSIM-model (version 7.7) was then used to simulate maize grain yield and dry matter production to assess risks associated with the production of maize/lablab intercropping. The results obtained from this study showed that maize/lablab intercropping had significant effects on measured parameters (grain, biomass yield soil water content, and N-minerals). Maize+lablab-28 produced 46 % higher grain yield than sole cropping (24%) and maize+lablab-ST) (30%). The results also showed variation in soil water content at different depths among the treatments. The soil water content was increased with depth. The intercropped plots and lablab sole had significantly higher soil water content than the sole maize. At all depths, the highest soil water content was obtained under sole lablab followed by maize+lablab-ST and maize+lablab-28. It was notable however that maize/lablab intercropping showed a higher NO3--N and NH4+-N levels at all depths. At both sites, the soil NO3--N showed a sharp drop at V7 sampling time. The results showed the benefits of intercropping in comparison to sole cropping as demonstrated by positive land equivalent ratios of >1 for both cropping systems in both years and sites. Modelling exercises showed that APSIM was able to simulate the results sufficiently. In the simulation experiment, a stronger negative effect of planting lablab with maize simultaneously was found. Hence, delayed planting of lablab should be a standard practice / NRF

Meat substitutes are an increasingly popular subject area for sustainability studies and industrial transition.  A transition to plant-based alternatives requires a substantial value chain with the ability to withstand transitional structure and market changes. While the value chain poses challenges at each level, considering the topmost level reveals unique market dynamics specific to the agriculture of legumes.  Western markets have shown a trend favoring plant-based protein as a sustainable source of nutrition however, research on raw material sourcing, processing, and its market structure is limited, often with low government intervention. The Indian agriculture sector comprises mainly legume farming, contributing to a significant portion of the country’s economy. This thesis aims to describe the actor interactions in the Indian agriculture sector with a focus on drivers and hindrances that promote and limit the growth of the legume agriculture technological innovation system.  Using qualitative research methods to gather data from candidates directly involved in the legume trade has proven insightful. Findings show that there are complex interaction scenarios between the actors involved. Restricted by the size of farming area, target markets, and market convention, farmers are subjected to a lock-in type scenario with respect to sales and purchasers. Although government intervention offers protection and assistance, it remains a safety net for the agriculture community. Authoritative influence is used to promote policy however, interactions between actors are not optimized to support this change effectively.  Inter-organizational and actor networks are weak apart from certain business relationships arising from contractual obligations. Knowledge development is present but formal procedures of knowledge diffusion are absent, leaving opportunities not being capitalized on. The study discusses the merits and demerits of this system using the Technology Innovation System framework functions for the analysis. Limitations of the theoretical framework is discussed as a modified version of the framework is utilized in this thesis, giving importance to interactions and localized application of TIS functions.

Thesis (Ph.D. Agriculture (Plant Production)) -- University of Limpopo, 2022 / Livestock has evolved to serve as the foundation and backbone of human well-being, and it is an important component of South Africa's agricultural sector. The small stock such as sheep (Ovis aries) in Limpopo province has remained a significant and multifunctional livelihood strategy for the majority of the rural and resource-poor people. Factors such as population growth, urbanization, rising per capita income and changes in consumer tastes and preferences are all contributing to gradual increases in livestock product consumption and demand. According to the 2019 Abstract of Agricultural Statistics, South Africa is an importer of sheep and sheep products. If the sheep production industry in the province could pursue this opportunity and realize its full production potential then increased production could stimulate economic growth and development, particularly from the communal and smallholder sector. Objective one of the study seeks to describe the demographic and socio-economic characteristics of communal and smallholder sheep farmers, identify sheep feeding practices and describe the constraints that hinder the sustainable productive growth of communal and smallholder sheep systems. Data were collected from one hundred and twenty (120) sheep farmers using a structured questionnaire across three agro-ecological zones of Limpopo province. Results revealed that overall, the majority of sheep farmers were males (78%) and farmers were above 60 years old (48%). Mean sheep flock size differed significantly between communal (24.74) and smallholder (62.36) farmers. Indigenous crossbreeds were the dominant breed kept by communal (86%) and smallholder (77%) farmers. The majority of communal and smallholder farmers (90% and 96%, respectively) reared their sheep under an extensive system with rangelands as the main source of feed. As a result, they experience a critical feed gap during June and September, the mid-winter to early spring until the first rains. The findings of the study revealed that feed shortages and diseases were ranked as the first and second production constraints by sheep farmers in both the production systems. In rangeland-dependent feeding systems, insufficient feed to meet animal demands create a feed gap, which is a critical factor that limits sheep productivity and causes xxi land degradation through overgrazing. Improved forages have been widely advocated as a critical step toward resolving this challenge. However, the adoption and utilization of improved technologies such as on-farm forage legume production by these farmers have been very low, contributing to the province's low sheep productivity. An extension of objective one of this study used primary data which was collected from a sample of 120 sheep farmers to determine the factors that influence the adoption of on-farm forage legume production and the perceived barriers to adoption by communal and smallholder sheep farmers in the Limpopo province. A Probit regression model and Principal Component Analysis (PCA) were used to analyze the data. The study revealed that the adoption of on-farm forage production by communal and smallholder sheep farmers is influenced by several factors, including gender, farming experience, knowledge of forage legume production, source of income, membership in farmer associations, access to extension services and farm size. Farmer perceived barriers to adoption of on-farm forage legume production identified by this study were low institutional support, lack of resources, lack of knowledge, shortage of water and objectives of the farmer. It is therefore recommended that intensive and high-quality extension support in partnership with industry associations and stakeholders is required for communal and smallholder farmers to improve forage technology awareness, training and promote on-farm forage production to transform communal and smallholder sheep feeding practices. In the face of climate change, identifying forage species with a high potential to mitigate winter feed gap challenges under more variable climatic conditions is critical. Trifolium and Vicia species are forage legumes well known for producing high-quality forage, particularly protein, which is deficient in the majority of feed resources used for sheep feeding during the winter season. Climate change-induced stresses from rising temperatures, which these winter annual forage legumes are likely to face, necessitate agronomic and breeding approaches to improve their adaptability. Lack of knowledge on how these climate change mitigation approaches influence the productivity of winter annual forage legumes in the Pietersburg Plateau of Limpopo province prompted objective two of this study. A three-year field experiment laid in a split-split plot design with four replications was conducted to measure the effects of planting date, cultivar and harvest stage on the physiological traits associated with biomass production, forage quality, nodulation activity and xxii nutritive value of annual clover and vetch species. The results showed that the planting date and harvest stage had a significant effect on leaf gaseous exchange and biomass production. A non-significant effect of planting date on nutritive value was observed. Intercellular CO2 concentration, transpiration rate, stomatal conductance, instantaneous water use efficiency and intrinsic water use efficiency in cultivars increased with delayed planting, while a decrease in photosynthetic rate, shoot DM, root DM and nodule DM was observed. Overall among the cultivars, Resal, Alex, Elite, Laser and Dr Baumans showed more consistency in terms of leaf gaseous exchange, biomass production and quality traits under planting date 1 and varying harvest stages. Investment in the year-round fodder flow establishment with high-quality forages is important in supporting sustainable sheep production. Forage legume-grass rotation systems are important not only for green fodder production of high crude protein, mineral and vitamin content throughout the year but also for enhanced soil fertility to reduce the nitrogen (N) fertilizer requirements. Accurate estimates of forage yields on the farm are required for fodder flow planning to ensure the seasonal distribution of fodder throughout the year. Objective three of the study was a no-tillage, short-term rotation experiment conducted to determine the growth and nutritive value of forage sorghum, planted after the winter annual forage legumes in combination with nitrogen application and to validate the performance of the APSIM-grain sorghum crop model in simulating forage sorghum growth and biomass production under different N rates. The treatments were planting date (January and February) and N source from inorganic N fertilizer (0 kg N ha-1, 60 kg N ha-1, 120 kg N ha-1, 180 kg N ha-1) and forage legume N residues (Alex, Capello, Dr Baumans, Elite, Hanka, Laser, Linkarus, Opolska, Resal and Timok) arranged in a randomized complete block design with four replicates. The findings of this study showed a significant response of forage sorghum growth and nutritive value to planting date. Delayed planting reduced plant height (11%), stem diameter (18%), LAI (6.7%), chlorophyll content (18%), NDVI (2.5%), photosynthetic rate (38%) and biomass production (8%). Delayed planting further reduced crude protein, acid detergent fiber and N yield. Nitrogen source from inorganic N at 60 kg N ha-1, 120 kg N ha-1, 180 kg N ha-1 and residual N from annual clover and vetch cultivars had a significant effect on morphological, physiological, yield and nutritive value parameters of forage sorghum. xxiii Generally, legume N residue effects on all the studied parameters of forage sorghum were similar to the inorganic N fertilizer of 60 kg N ha-1. However, the effects differed widely according to the species and cultivar of the legume. Resal, Laser, Elite Capello and Dr Baumans N residue consistently showed greater effects than other legume residues. They consistently outperformed inorganic 60 kg N ha-1 on the most measured parameters. The results confirm that annual clover-forage sorghum and vetch-forage sorghum rotation have huge potential to reduce the cost and negative environmental effects associated with inorganic N use in forage prediction systems. Regarding the evaluation of the potential of the APSIM grain legume model to simulate forage legume DM and plant height, in general, the model performed well and accurately in predicting the shoot dry matter accumulation and plant height under 0 kg N ha-1, 60 kg N ha-1 and 120 kg N ha-1. However, it underestimated both these parameters at 180 kg N ha-1 implying that the application of N up to 180 kg N ha-1 is not necessary. APSIM-grain module was able to accurately predict forage biomass production under N rates up to 120 kg N ha-1 and it is therefore considered reliable to support the N nutrition in the forage sorghum fodder production systems. / University of Limpopo, research office under the UCDP program and National Research Foundation-Thuthuka

The urgent action needed to achieve the goals within the Paris Agreement and restrict the increase of global warming to 1,5 degrees calls for adaptation across the whole of society. One way to reduce anthropogenic greenhouse gas emissions is to enable a transition towards a sustainable food system, from meat products to more plant-based products including the consumption of more legumes. Legumes have many positive effects, both for arable land in terms of nitrogen fixation, as well as for people’s health in terms of high bioavailability. However, many plant-based alternatives currently on the market are multi-processed, resulting in low bioavailability and a high use of non-natural additives. The aim of this study is to shed light on what enables and hinders the transition towards a sustainable food system based on a more plant-based diet of Swedish-grown crops, including legumes. Furthermore, the purpose is to explore opportunities and challenges within strategy documents that affect the transition toward more sustainable food production. Two Swedish companies, both selling products labeled From Sweden, were interviewed to increase the understanding of opportunities and challenges for them to thrive in the existing meat-dominated market. The Swedish Board of Agriculture was interviewed to increase the understanding of the policy environment. This was complemented by a content analysis of two policy documents, the Swedish Food Strategy and the EU Farm to Fork Strategy. The analysis of collected data showed that the development of legume-based, From Sweden products depends on both collaboration between actors in the value chain, and educating Swedish consumers. The findings also reveal that retailers play an important role when it comes to affecting the opportunities and challenges for a transition towards more plant-based diets. In light of this, the study concludes that education, retailers and collaboration are the most important factors at play when it comes to opportunities and challenges that enable or hinder the transition towards a more sustainable food system including more high-legume plant-based diets. Often, these factors can be seen as both opportunities and challenges. However, further research is needed to better understand how these factors fit into the wider policy environment, where more key authorities beyond the scope of this study are included.

No description available.

MRDV / Institute for Rural Development / This study originated from the realisation that non-conventional crops such as Bambara nuts (Vigna subterranea) were becoming increasingly important in addressing food insecurity and malnutrition in the smallholder farming sector of countries in sub-Saharan Africa. Moreover, some of the smallholder crop farmers were observed to be continuing to rely on indigenous techniques to forecast rainfall and adapt agricultural activities in response to climate variability. However, it was not clear how climate change influenced the productivity of V. subterranea. Nor were the indigenous approaches that farmers used to forecast rainfall on this phenomenon well understood. Thus, a study was carried out to identify and document indigenous approaches that smallholder farmers used to forecast rainfall and adaptation practices relating to V. subterranea. The study was conducted in Xigalo and Lambani villages located in Collins Chabane Local Municipality of Vhembe District in Limpopo Province. The villages served as case study areas that helped to compare the native approaches that the Va-Tsonga and Vha-Venda used to forecast rainfall in the course of producing V. subterranea. A multi-case study research design, which was exploratory in nature was adopted. Convenience and snowball sampling techniques were used to identify and select respondents. The triangulation of participatory methods, techniques and tools guided the collection of qualitative data. Key informant interviews, learning circles, photovoice, one-on-one interviews and narrative inquiry techniques were applied during data collection. Smallholder farmers and the elderly members of communities were the respondents. Nine key informants in Xigalo and Lambani villages were interviewed. One retired and two currently serving government extension officers were also interviewed. Separate learning circles comprising mainly elderly men and women were also organised. Each learning circle was made up of 7-10 respondents. Atlas.ti version 7.5.7 software was used to analyse the qualitative data following the thematic content analysis approach. It was observed that the respondents were aware of climate variability events that affected V. subterranea. Some of the events were shifts in rainfall patterns, heavy rainfall, extreme temperatures, scarcity of summer rainfall, the disappearance of lunar signs and the seasonal cycle variations. Eighteen types of phenological signs used to predict rainfall were identified. The most common signs included the Milky Way Galaxy of stars, musical sounds of birds and frogs, moon shapes, cumulus and cumulonimbus cloud types. A close relationship between conservation of V. subterranea and adaptation strategies was said to exist. It was evident that most commonly used conservation strategies were rainmaking ceremonies, planting after the summer rains, hoeing weeds, soaking seeds before planting, hilling or earthing up around the base of the V. subterranea plant and storing the legumes in traditional vessels and sacks. The need for integrating western scientific knowledge with native forecasts to inform the production of V. subterranea was uncovered. In addition to this, the needs of Tsonga and Venda communities should inform local policy interventions. Lastly, adaptation strategies that address food insecurity with V. subterranea being part of the agro-ecosystem deserve attention in scientific investigation and policymaking. / NRF

Since ancient times, indigenous plants have been used by traditional healers for treating various ailments. Sutherlandia frutescens is one of the most commonly used medicinal plants of southern Africa. This widely distributed plant has been traditionally used to treat cancer and HIV patients; however scientific validation is still in high demand. This research aimed to phytochemically characterise the various extracts prepared and to determine if any chemotypes were present. Subsequent biological characterisation was carried out to preliminary ascertain whether this medicinal plant could have anti-cancer and/or immunemodulating properties and which compounds might be responsible for these actions. Various traditional and organic extracts were prepared. Extracts, fractions and compounds generated were analysed and chemical profiles obtained. Column chromatographic techniques were used to isolate and purify compounds and structure elucidation was carried out using various analytical techniques. Sulforhodamine B and cytometric bead array assays were performed to determine the biological activities of samples generated. / Life and Consumer Sciences / (M. Sc. (Life Sciences)

Les prairies indigènes présentent une source importante d'alimentation pour le pâturage du bétail dans les prairies Canadiennes semi-arides. L'addition de légumineuses fixatrices d'azote et de phosphore dans les prairies indigènes peut améliorer la productivité et la valeur nutritive de fourrage. Ces pratiques peuvent induire des modifications de la structure et de la diversité des communautés fongiques du sol, ce qui peut en retour avoir un impact sur la production et le contenu nutritionnel du fourrage. L’objectif de cette étude était de développer un système de pâturage à bas niveau d’intrants, productif, autonome et durable. À court terme, nous voulions 1) déterminer l'effet des légumineuses (Medicago sativa, une légumineuse cultivée ou Dalea purpurea, une légumineuse indigène) et la fertilité en phosphore du sol sur la productivité et la valeur nutritive des graminées indigènes, comparées avec celles de la graminée introduite Bromus biebersteinii en mélange avec le M. sativa, 2) identifier l'effet de ces pratiques sur la diversité et la structure des communautés des champignons mycorhiziens à arbuscules (CMA) et des champignons totaux, 3) identifier l'effet des légumineuses et des CMA sur les interactions compétitives entre les graminées de saison fraîche et les graminées de saison chaude. Les expériences menées au champ ont montré que M. sativa améliorait les teneurs en azote et en phosphore des graminées indigènes au début de l'été, ainsi que la teneur en azote de la graminée de saison chaude Bouteloua gracilis à la fin de l'été de l'année sèche 2009. Par contre, la fertilité en phosphore du sol n'ait pas affecté la productivité des plantes. D'autre part, l'inclusion des légumineuses augmentait la diversité des CMA dans le mélange de graminées indigènes. Cette modification présentait des corrélations positives avec la productivité et la quantité totale d'azote chez le M. sativa et avec la teneur en phosphore des graminées indigènes, au début de l'été. La structure des communautés de champignons totaux était influencée par l'interaction entre le mélange des espèces et la fertilité en phosphore du sol seulement en 2008 (année humide). Cet effet pourrait être lié en partie avec la productivité des plantes et l'humidité du sol. Les expériences menées en chambre de culture ont montré que les CMA peuvent favoriser la productivité des graminées de saison chaude au détriment des graminées de saison fraîche. En effet, Glomus cubense augmentait la productivité de la graminée de saison chaude B. gracilis, en présence de M. sativa. Cet effet pourrait être associé à l’effet négatif du G. cubense sur la fixation de l’azote par le M. sativa et à la diminution de l’efficacité d’utilisation de l'azote de certaines graminées de saison fraîche résultant en une augmentation de la disponibilité de l'azote pour B. gracilis. Par contre, le Glomus sp. augmentait la biomasse de Schizachyrium scoparium, autre graminée de saison chaude, en absence de légumineuse. Ce phénomène pourrait être attribuable à une amélioration de l’efficacité d’utilisation du P de cette graminée. En conclusion, mes travaux de recherche ont montré que la légumineuse cultivée M. sativa peut améliorer la valeur nutritive des graminées indigènes au début de l'été ainsi que celle de la graminée de saison chaude B. gracilis, dans des conditions de sécheresse sévère de la fin de l'été. De plus, l'addition de M. sativa dans le mélange de graminées indigènes peut contribuer à augmenter le nombre des espèces bénéfiques des CMA pour la production et la nutrition du fourrage au début de l'été. / The native grasslands are considered as the main feed source for livestock grazing, in semi-arid regions of the Canadian prairies. The addition of N fixing legumes and phosphorus to semi-arid native grasslands may increase the productivity and nutritive value of forage. However, these practices may also shape the structure and diversity of soil fungal communities which in turn may impact forage production and nutritive value. The global objective of this research was to design productive, self-sustaining, permanent and with low inputs pastures. The specific objectives were 1) to demonstrate the effect of N-fixing legumes (the cultivated legume Medicago sativa or the native legume Dalea purpurea) and soil P fertility on the productivity and nutritive value of native grasses mixes in comparison to the mixture of the introduced grass Bromus biebersteinii and M. sativa, 2) identify the effect of these practices on the diversity and community structure of arbuscular mycorrhizal (AM) fungi and total fungi, and 3) identify the effect of legumes and AM fungi on competitive interactions between native cool-season grasses and native warm-season grasses. The field experiment showed that M. sativa improved the nitrogen and phosphorus concentrations of native grasses mixes early in the summer, as well as the N concentration of the warm-season grass B. gracilis, in late summer of the driest year 2009. In contrast, the soil phosphorus fertility had no effect on plant productivity. On the other hand, the inclusion of legumes to the mix of native grasses generally increased AM fungal diversity. This shift was positively correlated with the productivity and nitrogen uptake by M. sativa and with the phosphorus concentration of native grasses mixes in early summer. The structure of the total fungal community was affected by the interaction between species mixtures and soil P fertility only in the wet year (2008), suggesting that this effect was likely driven in part by plant productivity and soil moisture. The growth chamber experiment showed that the AM fungi may favoured the growth of warm-season grasses under competition with cool-season grasses. However, Glomus cubense increased the productivity of warm-season grass B. gracilis when growing with M. sativa. This effect might be related to a negative impact of G. cubense on the nitrogen-fixing activity of M. sativa and to a lower N-use efficiency of certain cool-season grasses, which resulted in increased soil N availability for B. gracilis. In contrast, Glomus sp. enhanced the growth of S. scoparium, another warm-season grass in the absence of legumes, and this may be related to improved P-use efficiency in this grass. We concluded that the cultivated legume M. sativa can improve the nutritive value of native grasses mixes early in the summer and also of warm season grass under severe drought conditions in late summer. In addition, the inclusion of M. sativa within native grass mixes may contribute to promote beneficial AM fungi taxa that were involved in forage production and nutrition early in the summer.

Made available in DSpace on 2016-08-10T10:44:45Z (GMT). No. of bitstreams: 1 MARIA CECILIA ALVES DE VASCONCELOS.pdf: 1811097 bytes, checksum: 7225cbf224da270e10a80f038703183e (MD5) Previous issue date: 2015-03-11 / Among the parameters used by the scientific community, the biomass evaluation is the most precise one in terms of presenting the biological components of the soil. Intending to evaluate the potential of the legumes Campo Grande Stylo, calopo (Calopogonium mucunoides) and pigeon pea regarding the recovery of damaged soil, sixteen plats of these legumes were planted in latin square form for later microbial biomass analysis. The microbial biomass was analyzed according to the Vance et. (1987) principles. No significant differences were found compared to the fallow, although circumstances like the lack of precipitation, the time of the year and the production of dry matter affected the microbial biomass development. The studied legumes that presented the largest microbial biomass production were the Campo Grande Stylo and the calopo (Calopogonium mucunoides), showing a trend of improvement compared to the fallow. Despite the stress factors, the microbial biomass showed a trend of improvement on the plats where the legumes were seeded, and it can be considered a prior parameter of agrisystem changes. / Dentre os parâmetros utilizados pela comunidade científica, o que apresenta maior sensibilidade na caracterização dos componentes biológicos do solo é a avaliação de biomassa microbiana (BMS). Com o objetivo de avaliar o potencial das leguminosas Calopogônio, Estilosantes e feijão Guandu na recuperação dos solos degradados e na melhoria da biomassa microbiana, foi realizado o plantio das leguminosas e posterior análise da biomassa microbiana em dezesseis canteiros distribuídos em um quadrado latino. A biomassa microbiana foi analisada conforme os princípios de Vance et. (1987). Não foram encontradas diferenças significativas em relação ao pousio, porém fatores como a baixa pluviosidade, época de plantio e produção de massa seca influenciaram o desenvolvimento da BMS. As leguminosas estudadas que apresentaram maior produção de BMS foram o Estilosantes e o Calopogônio, evidenciando uma tendência de aumento em relação ao pousio. Mesmo com fatores de estresse, a biomassa microbiana nos canteiros com as leguminosas evidenciou um potencial de aumento, podendo ser considerado um parâmetro que antecede alterações no agrosistema.