Orientadores: Valdemiro Carlos Sgarbieri, Durvanei Augusto Maria / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos / Made available in DSpace on 2018-08-09T10:14:07Z (GMT). No. of bitstreams: 1 Castro_GeorgiaAlvaresde_D.pdf: 11428319 bytes, checksum: 35439f6a0fe2edb2363b7aa078ebd0fa (MD5) Previous issue date: 2007 / Resumo: O principal objetivo deste projeto foi avaliar as propriedades fisiológicas potenciais de hidrolisados de colágeno (HC) e de um isolado protéico de soro de leite bovino (WPI) em relação aos efeitos anti-ulcerativo e anti-tumoral em roedores. O projeto apresenta uma parte tecnológica que envolve a produção do WPI e hidrolisados de colágeno (Gelita) com diferentes distribuições de massa molecular. Para obter as frações protéicas utilizadas neste estudo, responsáveis por estes atributos, uma caracterização foi conduzida por fracionamento através de microfiltração utilizando tecnologia de membranas e sistema FPLC por cromatografia de permeação em gel e de fase reversa. Estas tecnologias foram utilizadas para obtenção dos hidrolisados de colágeno com diferentes massas moleculares e do isolado protéico de soro de leite (WPI), para possibilitar a caracterização química e avaliações bioquímica/ fisiológica em suas atividades anti-úlcera e anti-tumoral. Os produtos elaborados a partir desta tecnologia de fracionamento foram caracterizados de acordo com o grau de hidrólise, composição de aminoácidos, massa molecular e número de peptídios, utilizando metodologias estabelecidas. Hidrolisados de colágeno bovino e suíno apresentaram características diferentes quanto ao grau de amidação e teor de amônio total, assim como na eletrofocalização. Os perfis cromatográficos de permeação em gel foram semelhantes, mas a atividade biológica em meio com células de melanoma B16F10 também diferiu. A combinação destas duas proteínas para avaliação dos efeitos anti-ulcerativo e anti-tumoral, também foi utilizada. Atenção especial foi dedicada a uma melhor compreensão da rota metabólica e mecanismos de ação destas duas proteínas e seus peptídios nos ensaios biológicos (modelo com ratos e camundongos, respectivamente). Para investigar o efeito anti-ulcerativo, ratos Wistar com 250-300g de peso corpóreo foram utilizados. Experimentos com dose única e repetitivas foram feitos para avaliar os efeitos destas proteínas na redução ou inibição da úlcera gástrica. A participação das substâncias sulfidrila ativas provenientes da glutationa (GSH), aminoácidos e ligações inter e intra- cadeias polipeptídicas, no mecanismo de proteção, foi estudada através da alquilação total do grupo reativo SH pela Netilmaleimida (NEM). Algumas respostas fisiológicas foram avaliadas como a produção de muco gástrico e gastrina durante o modelo experimental de úlcera induzida por etanol. A atividade inibitória em modelo de úlcera gástrica induzida por etanol foi também distinta nos hidrolisados bovino (BCH) e suínos (PCH), apresentando o hidrolisado suíno em torno de 60% de inibição das lesões ulcerativas quando administrado em dose única 750mg/Kg de peso corpóreo. No experimento de dose dupla a porcentagem de inibição das lesões ulcerativas gástricas dos hidrolisados bovinos foi maior que 70% quando administrado 1500 mg/Kg pc. Quando foram realizadas combinações de hidrolisados de colágeno bovino ou suíno com WPI, obteve-se uma combinação otimizada para BCH:WPI (50:50) na concentração de 375mg/Kg, com mais de 70% de inibição das lesões ulcerativas gástricas. Os hidrolisados de colágeno levaram ao aumento da produção de muco na mucosa gástrica e o WPI à inibição com a participação das substâncias sulfidrila não protéica na atividade inibitória das lesões gástricas. Na avaliação in vitro com as células B16F10 e os hidrolisados de colágeno bovino e suíno e suas frações obtidas por micro e ultrafiltração, assim como aquelas provenientes da cromatografia por HPLC de fase reversa, mostraram que estas últimas apresentaram a menor concentração inibitória para 50% das células (IC50), também apresentando aumento na fase de apoptose com significativo aumento na porcentagem de expressão da caspase-3 nos meios contendo as frações F1, F2 e F3. O estudo de propriedade anti-tumoral foi conduzido utilizando camundongos C57BL/6J. Animais saudáveis foram injetados (transplantados) com células tumorais de melanoma murino B16F10 na área dorsal para avaliar a implantação tumoral, progressão, caquexia observando a perda de peso e outros sintomas, metástase, capacidade proliferativa do tumor assim como sua classificação de acordo com o volume, tamanho e número. Os mecanismos nos quais a dieta protéica pode estar envolvida, avaliando a fase do ciclo celular, apoptose e efeitos anti-proliferativos no tecido tumoral foram também objetivos deste estudo. Protocolos utilizando quimioterápico foram seguidos com aquelas dietas protéicas que apresentaram melhor resposta contra a tumorigênese considerando outros mecanismos que poderiam estar envolvidos. No modelo de tumorigênese in vivo com células de melanoma B16F10 observouse uma menor porcentagem de mortalidade, maior porcentagem de sobre-vida e aspectos macroscópicos vantajosos para o grupo de animais C57BL/6J que ingeriram American Institute of Nutrition (AIN-93) tendo como fonte protéica BCH: WPI (50:50) e tratamento quimioterápico. O ciclo celular também sofreu alteração com as diferentes dietas. A fase G0/G1 tanto no grupo com tratamento quimioterápico como no grupo sem quimioterápico foi significativamente aumentada para os grupos dietéticos com BCH e BCH-P1 + WPI. A porcentagem de apoptose aumentou para BCH+WPI com tratamento quimioterápico. A capacidade proliferativa das células B16F10 diminuiu para os grupos de camundongos C57BL/6J que se alimentaram de hidrolisados de colágeno sem tratamento quimioterápico e quando houve quimioterapia associada somente aqueles grupos que receberam a combinação das duas proteínas BCH ou BCHP1 com WPI tiveram uma diminuição significativa da capacidade proliferativa das células B16F10 / Abstract: The main objective of this project was to evaluate the potential physiological properties of collagen hydrolysate (CH from Gelita) and a whey protein isolate (WPI) in relation to anti-ulcerative and anti-tumoral effects in rodents. The project presents a technological part, which involves the WPI production with different molecular masses. To obtain the proteic fractions used in this study responsible for these attributes, a characterization was conducted using fractionation through microfiltration using membranes technology and FPLC system through gel permeation and reverse phase chromatography. These technologies were used to obtain the collagen hydrolysate with different molecular masses and the whey protein isolate (WPI) to allow the chemical characterization and biochemical and physiological evaluations of their anti-ulcerative and anti-tumoral activities. The elaborated products obtained from these fractionation technologies and also the products from GELITA (derivatives of collagen) were characterized according to degree of hydrolysis, amino acids composition, molecular mass and peptide number, using established methodologies. Bovine and porcine collagen hydrolysates presented different characteristics concerning degree of amidation and total ammonium content, as well as in isoelectric focusing. The chromatographic profiles in gel were similar, but the biological activity in cellular medium of melanoma B16F10 also differed. Combination of these two types of proteins to evaluate anti-ulcerative and antitumoral effects were also done. Special attention was dedicated to better understanding of metabolic routes and mechanisms of action of these two proteins and their peptides in biological assays (models with rats and mice, respectively). To investigate the anti-ulcerative effect Wistar rats (250-300g bw) were used. Experiments with single and double doses were conducted to evaluate the effect of these proteins in reduction or inhibition of gastric ulcer. The participation of active sulfhydryl compounds, from gluthatione, aminoacids and inter-, intra- polypeptides chains, in protection mechanism, was studied through alkylation of SH reactive groups using N-ethylmaleimide (NEM). Some physiological responses were evaluated, as mucus and gastrin production, during experimental model of ulcer induced by ethanol. The inhibitory activity in gastric ulcer model induced by ethanol was also distinct for these two hydrolysates, bovine (BCH) and porcine (PCH). The porcine hydrolysate presented around 60% of ulcerative lesion inhibition when 750mg/Kg body weight was administrated at single dose. For double dose experiment the percentage of inhibition for bovine hydrolysate was higher than 70% when 1,500mg/Kg bw was administrated. When was tested combinations of either bovine or porcine collagen hydrolysate with WPI a optimized combination was obtained BCH:WPI (50:50) at 375mg/Kg bw of concentration presenting more than 70% gastric ulcerative lesion inhibition. The collagen hydrolysate led to increased mucus production in gastric mucosa while WPI caused in addition an inhibition by non-proteic sulfhydryl compounds participation in the inhibitory activity of gastric lesions. In vitro evaluation using B16F10 cells, the bovine and porcine collagens hydrolysates and their fractions obtained through micro and ultrafiltration, as well as those obtained by reverse phase chromatography (F1, F2, F3), showed the lowest inhibitory concentration for 50% of cells (IC50), presenting increased apoptosis percentage and a significant increase of caspase-3 expression. The study of anti-tumoral property was conducted using mice C57BL/6J. Healthy animals were injected (transplanted) with tumoral cells of murine melanoma B16F10 in dorsal area to evaluate the tumoral implantation, progression, caquexia observing the body weight loss and other symptoms, metastasis, proliferative capacity of the tumor, as its classification according to volume, size and number. The mechanisms involving the protein in the diet for evaluating cell cycle phases, apoptosis and anti-proliferative effects in tumoral tissue development were also objectives of this study. Protocols using chemotherapic were followed with protein which presented better response against tumorigenesis considering other mechanisms that could be involved. In tumorigenesis, in vivo model using B16F10 melanoma cells, a lower percentage of mortality, longer survival percentage and better macroscopic aspects were observed for C57BL/6J animal groups, fed American Institute of Nutrition (AIN-93) with BCH:WPI (50:50) as protein source plus chemotherapic treatment. The cellular cycle also suffered alteration with different diets. The G0/G1 phase for both groups treated and non-treated with chemotherapic increased significantly for the dietetic groups BCH or BCH-P1 + WPI. The apoptosis percentage increased for BCH+WPI+ChTP (with chemotherapic treatment). The proliferative capacity of B16F10 cells was reduced in the groups fed collagen hydrolysate without chemotherapic treatment and when the chemotherapic was associated only the groups that received both proteins BCH or BCH-P1 with WPI showed a significant reduction of proliferative capacity of these cells / Doutorado / Nutrição Experimental e Aplicada à Tecnologia de Alimentos / Doutor em Alimentos e Nutrição

O presente trabalho tem por objetivo traçar um panorama da vida do poeta Murilo Mendes na Europa entre as décadas de 1950 e 1970, através das cartas enviadas por ele a sua irmã Virgínia Mendes Torres. Com base na análise desse conjunto epistolar é possível refazer parte da trajetória do poeta mineiro que transplantado em território estrangeiro atuou nos meios acadêmico, literário e artístico, representando o Brasil e construindo sua própria vertente do modernismo brasileiro. / The current work aims to draw a prospect of the poet Murilo Mendes life in Europe among the decades of 1950 and 1970, through the letters sent by him and his sister Virgínia Mendes Torres. Considering the analysis of that epistolary group is possible to redo part of the poet\'s trajectory that transplanted in foreign territory acted in the academic, literary and artistic means representing Brazil and building his own view of the Brazilian modernism.

Orientador: Antonio Alcir Bernárdez Pécora / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Estudos da Linguagem / Made available in DSpace on 2018-08-21T22:43:30Z (GMT). No. of bitstreams: 1 Nascimento_AnaPaulaGomesdo_M.pdf: 2754234 bytes, checksum: 1c9defaf457c01056a6d8eb8de95bc5c (MD5) Previous issue date: 2012 / Resumo: O estudo das Cartas chilenas, obra atribuída a Tomas Antonio Gonzaga (1744- ca.1810), e proposto através da analise desta como obra inserida numa instituição retórica, reguladora das praticas de representação ate o século XVIII. Para isso, faz-se necessário uma investigação acerca do gênero da obra, que identificamos como uma epistola satírica. De fato, o próprio título dela revelaria o seu caráter de epistola, ao passo que o seu conteúdo demonstraria a sua natureza satírica. Porem, o levantamento da trajetória da recepção critica da obra - realizada por FURTADO em 1997 - evidencia que, de maneira geral, tendeu-se a classificá-la principalmente como documento historiográfico capaz de revelar os antecedentes da Inconfidência Mineira, do que como documento literário dotado de formas historicizadas. Desse modo, a tradição critica parece ter se concentrado em possíveis eventos históricos, sem, no entanto, levar em conta a historicidade do aspecto retórico-ético-poético da obra. O trabalho efetua, portanto, uma leitura retoricamente orientada das cartas e espera contribuir para uma nova abordagem da obra. São estudadas as técnicas da ecfrase e da etopeia, mostrando-se como são significativas as "caricaturas" presentes nas Cartas chilenas. Busca-se demonstrar que na obra coexistem as imitações icastica e fantástica, de modo que em algumas cartas a ênfase recai sobre os aspectos histórico-particulares e em outras sobre os poéticos/universais. Estuda-se, ainda, a presença da tópica do soldado fanfarrão, o miles gloriosus da comedia romana ou o alazon da comedia grega. Aproxima-se, por fim, as figuras de Fanfarrão Minesio e de Dom Quixote / Abstract: The present study proposes to analyze Chilean Letters, a work attributed to Tomas Antonio Gonzaga (1744-ca.1810), in terms of its place within a rhetorical institution, which regulated representation practices until the 18th century. Thus, an investigation regarding the genre of the work is necessary, which we identify as a satirical epistle. In fact, the title itself reveals its epistolary nature, while its content demonstrates its satirical nature. However, a survey of the trajectory of the critical reception of the work - carried out by Furtado in 1997 - evidences that, in general, it tended to be classified mainly as a historiographical document that could reveal the events leading up to the Minas Conspiracy, rather than as a literary document with historicized patterns. Therefore, the critical tradition seems to have concentrated on possible historical events, without, however, taking into account the historicity of the rhetorical-ethical-poetic aspect of the work. The present study thus carries out a rhetorically-guided reading of the letters and intends to contribute to a new approach to the work. The techniques of ekphrasis and ethopoeia are studied, revealing the significance of the "caricatures" present in Chilean Letters. We aim to demonstrate that icastic and phantastic imitations coexist in the work, such that in some letters the emphasis falls on historical/specific aspects and in others on poetic/universal aspects. The presence of the topic of the braggart soldier is also studied, miles gloriosus in Roman comedy or alazon in Greek comedy. Finally, the figures of Fanfarrao Minesio and Don Quixote will be approximated / Mestrado / Teoria e Critica Literaria / Mestra em Teoria e História Literária

Living with Star Trek investigates the connections between Star Trek fandom and the Star Trek text. This study identifies and examines the American themes of utopia, community and self-improvement inherent within the fictional text which also form the thematic framework for letters written by fans to express their affection for the series. These letters represent a `network of support', whereby a community of fans is able to communicate with each other through written correspondence sent to producers, edited collections, and fan magazines. In talking about the series, fans confess and share intimate stories, often based around trauma or bereavement, and at the same time describe how Star Trek has played an important and inspirational part in their daily lives; Star Trek's utopian vision and communal spirit has given them the impetus to enact positive change. Drawing together the themes identified in the text and fan letters, the first half of the thesis examines Star Trek's use of history, narrative and myth to tell its futuristic stories. In particular, I examine how Star Trek has used the distinctive literary tradition of the Puritan American Jeremiad to create a didactic narrative that emphasises the attainment of utopia through communal effort and personal change. The second half of the thesis continues this inquiry by examining a range of letters that describe how fans are able to tap into the open nature of the Star Trek text and use it to fulfil needs and desires in their own daily lives. In particular, I stress how the letters are not just examples of fan affection but also represent a reciprocal relationship where fans can criticise and engage with the programme as well as use it as a form of motivation.

Doctorat en philosophie et lettres / info:eu-repo/semantics/nonPublished

This is a textually complete and comprehensively annotated edition of the poet Arthur Hugh Clough’s letters to five of the leading American poets and scholars of his day: Ralph Waldo Emerson, Charles Eliot Norton, James Russell Lowell, Francis James Child and Henry Wadsworth Longfellow, over the period 1847–1861. Fifteen of these letters have not previously been published, and those that appear in published editions are largely incomplete and unannotated. The letters in this edition have been transcribed from the original manuscripts held at the Bodleian and Houghton Libraries. They provide a great deal of valuable information about the less well-known later period of Clough’s life and have been extensively annotated to modern scholarly standards using information from primary literary and historical sources. The introduction to the thesis contextualises Clough’s visit to America and the initiation of the correspondence with his American friends, highlighting the central importance of the ‘American dimension’ to Clough’s life and work. I also discuss aspects of nineteenth-century letter-writing that have only relatively recently become the subject of critical attention, such as the impact of material factors – postage rates, steamship schedules, etc – on Clough’s transatlantic correspondence. Clough’s creation of an ‘epistolary self’ in his private letters, together with his distinctive habit of writing ‘journal-letters’ and the idea of letters as historical ‘testimony’ are the subject of detailed analysis, and I draw a number of parallels with his use of the epistolary form in his major poetry. Chapter 2 of the thesis evaluates existing ‘theories’ of annotation, reviews current practice in relation to the annotation of nineteenth-century correspondence and concludes with a reflection on my own experience of editing Clough’s letters. The absence of a definitive version of Clough’s American letters and the comprehensive introduction will make this edition an original contribution to scholarly work on nineteenth-century correspondence and poetry.

This thesis will apply performance criticism to ancient Jewish letters in order to answer two connected questions. First, how do we adequately describe the form and function of letters as they were read in antiquity in order to be able to define the genres of letters in a more precise way and second, to consider how performance theory in conjunction with other approaches can be applied to ancient letters. In order to address these concerns, we will include examples of free-standing letters from Elephantine, embedded Hebrew and Aramaic letters, and embedded Greek letters. By studying these texts, we will gain a substantial perspective on the variety of Second Temple period letters and we will be able to consider how probing the form and function of those letters may be applied for a better understanding of MMT. The intent of this inquiry is to help explain how MMT, or a section thereof, may have been performed in various situations and thereby provide a clearer view of the genre(s) of MMT.

Poet/ Editor/ Publisher is an annotated edition of the selected correspondence of Sylvia Beach, publisher and owner of the Shakespeare and Company bookstore, the writer and editor Bryher (Annie Winifred Ellerman), and her partner, the poet H.D. (Hilda Doolittle). The years covered by this selection, 1918 to 1931, are some of the most prolific for these women and for modernism. Beach published James Joyce’s Ulysses, H.D. wrote several books of poetry and prose, Bryher established POOL Productions and Close Up, the first magazine devoted to film criticism, and much more. The relationships fostered among H.D., Bryher, and Beach express an unconventional model for creative production—one more concerned with helping each other than making a profit. This model is expressed not only in Bryher’s publishing endeavors and financial support of Shakespeare and Company and other artists in her sphere, but also in the well-documented sacrifices Beach made to bring out Ulysses. Chatty and endearing, the letters demonstrate the way these relationships passed seamlessly from social to professional and back again. They are full of gossip, but also valuable professional advice and encouragement. For Bryher and H.D., who lived in Territet, Switzerland, Beach provided an essential connection not only to a major center of avant-garde art, but also, and more practically, to the mechanisms of distributing modernist writing: publishers, editors, literary journals, and printers. This dissertation joins a recovery of the work of women in the early twentieth century as well as a reconsideration of the roles each woman played in developing the modernist canon. These letters offer evidence of the influence of each woman’s efforts on an international network of artists and insight into the labor behind the great works of modernism.

Thesis advisor: Maia McAleavey / A time of rapid industrialization and burgeoning consumerism, the nineteenth century was full of things, a physical reality that is mirrored in the heavily material story worlds of Victorian literature. My dissertation investigates how objects do things in texts, exhibiting a mattered, agentic existence that decenters the human and proposes a materially-centered textual reality. In the writings of Charlotte Brontë, Charles Dickens, George Eliot, Wilkie Collins, and others, a particular set of objects-portraits, dresses, dolls, and letters-is characterized by their shared representation of the human body and the ways in which they act with, against, and independently of the characters they represent. These texts and objects emphasize the essential material components of textual realities and the ways in which objects have agency within the narrative to redefine the mattered framework of the text. The objects in this study operate on a spectrum of agency that emphasizes their role as active matter in their parent text. Going beyond the historical and cultural models that usually inform readings of things in Victorian literature, I investigate how these objects are active in upending the primacy of the human and constructing new assemblages of possibility and potentiality that cannot be accessed by the human alone. Each chapter traces the development of the agentic object in one or more texts as they reshape the structure of their fictional reality to allow objects to exist alongside with, rather than subservient to, their human creators and audiences. Acknowledging the ways in which things in texts have functioned historically and culturally in the nineteenth century, this dissertation examines how they operate textually, offering a differently centered narrative world that reimagines the role of objects as primary actors in constructing fictional realities. / Thesis (PhD) — Boston College, 2018. / Submitted to: Boston College. Graduate School of Arts and Sciences. / Discipline: English.

“Parsing Truth in Merovingian Gaul: Evidence and the Early Medieval Critic” considers how people distinguished truth from falsehood in a set of post-Roman kingdoms occupying much of modern France and western Germany from c. 450 to 751. Using Merovingian saints’ lives, legal documents, law codes, letters, and theological and philosophical texts, I consider how people and institutions navigated the possibility that information might be presented with the intent to deceive, or might just be wrong. Responses to questions about the reliability of information ranged from the practical to the abstractly epistemological, and the period produced multiple and contradictory arguments about how knowledge could, indeed, be certain. The dissertation concludes by examining some points of contact between Merovingian critical practices and Early Modern ones, looking specifically at the management of knowledge about relics at Sens.