Low-density parity check (LDPC) codes are very popular among error correction codes because of their high-performance capacity. Numerous investigations have been carried out to analyze the performance and simplify the implementation of LDPC codes. Relatively slow convergence of iterative decoding algorithm affects the performance of LDPC codes. Faster convergence can be achieved by reducing the number of iterations during the decoding process. In this thesis, a new approach for faster convergence is suggested by choosing a systematic parity check matrix that yields lowest Second Smallest Eigenvalue Modulus (SSEM) of its corresponding Laplacian matrix. MATLAB simulations are used to study the impact of eigenvalues on the number of iterations of the LDPC decoder. It is found that for a given (n, k) LDPC code, a parity check matrix with lowest SSEM converges quickly as compared to the parity check matrix with high SSEM. In other words, a densely connected graph that represents the parity check matrix takes more iterations to converge than a sparsely connected graph.

Various technologies depend on interfacial events that are influenced by various molecular interactions at a solid-liquid interface. The functionality of a surface plays an important role in many applications such as catalysis, sensing, and bio-compatibility, which can benefit from distinctive chemical and physical surface properties. To create tailor-made functional surfaces, surface host-guest assemblies based on Langmuir-Blodgett and self-assembly technique have been employed as a model system as they may offer the potential ability to regenerate surface properties via intercalation of various functional guest molecules. This thesis ranges over the development and characterization of host-guest assemblies and their feasibilities for the regeneration of surface properties via intercalation of functional guests. In our work, 3-dimensional host structures with cavities are constructed on a targeted solid substrate using Langmuir-Blodgett and self-assembly techniques. In particular, by adopting the fundamental concept of host-guest interaction in supramolecular chemistry, we expect that structurally homologous guest molecules where functional groups are anchored can be intercalated into the cavities between hydrophobe arrays at the liquid-solid interface from solution under well-controlled conditions. This approach offers the potential of separating the functional of the monolayer from the inherent structure of the host. The first part of this thesis details two-dimensional host-guest assemblies consisting of guanidinium (G), octadecylsulfonate (S) and various functional alkane guests at the air-aqueous interface and following deposition onto solid substrates via the Langmuir-Blodgett technique. In particular, we evaluated the stability of the host-guest assemblies and the feasibility of exchanging molecular guests under exposure to various organic solvent environments. Analysis of X-ray reflectivity measurements of the thin films showed that good stability of the host-guest assembly could not be achieved due to weak interactions between the host monoalyer and the solid surface. In addition, no evidence of intercalation of guest molecules into guest-free host-cavities was observed. The second part of this thesis discusses the effective methodologies to prepare low-density self-assembled monolayers (LDSAMs) with cavities on silicon substrates. We employed a step-wise reaction based on hydrolytic or silane chemistry: integral spacer molecules such as anthracene-derivatives were anchored to the Si substrate and then long alkane chains were appended to the spacer molecules. The results showed that LDSAMs using an anthryl spacer are attached at the SAM/Si interface via a Si-O-C linkage, and the films do not exhibit a densely packed monolayer quality as would be expected for a non-sterically hindered alkyltrichlorosilane on Si. Thus, the resulting LDSAMs (with cavities) may be capable of accommodating other guest molecules with hydrocarbon chains through intercalation in order to form host-guest assemblies. The third part of this thesis demonstrates the ability of LDSAMs to produce functional surfaces via the intercalation of various functional guest molecules. Self-assembled monolayers of (10-octadecyl)-9-anthracenethiol (host-SAMs) on Au substrates were prepared. Quartz crystal microbalance with dissipation (QCM-D) measurements was used to demonstrate the capacity of LDSAMs to confine guest molecules in the cavities and to probe the structural changes of the host-guest assembly during guest intercalation from ethanol solution. X-ray photoelectron spectroscopy (XPS) measurements were then used to probe host-guest monolayers formed by immersing the host monolayer in solutions in a variety of other solvents. A combined study of QCM-D and XPS showed that guest molecules were intercalated into host-cavities. The reversibility of the intercalation process allows a guest already situated in a host-cavity to be replaced with second guest under well-regulated solvent conditions. / Ph. D.

Extracellular vesicles (EV) originate from various cell types and reflect the contents of the originating cells. EVs are ubiquitous in nearly all body fluids, including blood plasma, and exhibit significant potential as biomarkers in disease diagnostics. However, isolating EVs from blood plasma remains challenging due to the lack of a standardised method. This study aimed to compare and optimize a density gradient ultracentrifugation workflow (DUC) against size exclusion chromatography-cation exchange chromatography (SEC-CEC) and evaluate SEC versus SEC-CEC. Common contaminants during isolation include lipoproteins (LP); previous studies have shown that lowering the pH of blood plasma can precipitate LP, enhancing isolation efficiency. Acidified blood plasma was compared with neutral plasma for EV isolation using all above mentioned methods. To assess the ability of the isolation methods to purify contaminants while retaining maximal EV yield, samples were analysed using multiple techniques, including particle quantity, free proteins, LP-associated apolipoprotein B, purity index (μg protein/particle), and EV-associated surface markers. The results indicate potential for DUC, but further optimization is necessary to improve the method and its isolation of EV. SEC-CEC emerged as an effective method, reducing contaminants by 71% (SEC) to 99% (SEC-CEC), increasing purity by 80%, and yielding positive signals from EV markers (SEC-CEC). The effect of acidification was ambiguous, it reduced apolipoprotein-B levels in plasma pre-isolation. However, post- isolation, neutral plasma exhibited significantly lower contaminations, albeit at the expense of total particle content and risking EV loss. The study underscored several advantages of SEC-CEC but indicated that acidification did not optimise isolation efficiency.

La thérapie cellulaire pourrait représenter une alternative à la transplantation hépatique dans certaines pathologies comme les maladies métaboliques sévères. Toutefois, la pénurie de donneurs d’organes implique la nécessité de trouver de nouvelles sources de cellules hépatiques comme les cellules souches pluripotentes qui peuvent être amplifiées extensivement et différenciées en tout type cellulaire. Les cellules souches embryonnaires humaines (hESC) et les cellules souches pluripotentes induites humaines (hiPSC) générées à partir des cellules somatiques de patients puis différenciées en hépatocytes représentent une source potentielle d’hépatocytes. Ces cellules permettent en outre d’envisager la transplantation d’hépatocytes autologues génétiquement modifiés comme alternative à la transplantation hépatique pour le traitement de certaines maladies génétiques du foie. L’hypercholestérolémie familiale (HF) est une maladie autosomale dominante due à des mutations dans le gène codant le Récepteur aux Low Density Lipoproteins (RLDL) qui est à l’origine d’un taux élevé de cholestérol sanguin de patients HF. Les patients homozygotes doivent épurer leur sérum par LDL-aphérèse en moyenne deux fois par mois dès le plus jeune âge pour éviter les infarctus mortels survenant dès l’enfance. Les hépatocytes différenciées à partir des iPSC de patients et leur correction in vitro, permettent d'évaluer la faisabilité de la transplantation d'hépatocytes autologues génétiquement modifiés pour le traitement de l’hypercholestérolémie familiale.Au cours du développement du foie, des hépatocytes et des cholangiocytes, les deux types de cellules épithéliales hépatiques, dérivent de progéniteurs hépatiques bipotents (les hépatoblastes). Bien que les cholangiocytes formant les canaux biliaires intrahépatiques ne représentent qu'une petite fraction de la population cellulaire totale du foie (3%), ces cellules régulent activement la composition de la bile par réabsorption des acides biliaires, un processus qui est important dans des maladies choléstatiques du foie. Dans la première partie de cette étude nous avons mis au point une approche de différenciation des cellules souches pluripotentes (hESC et hiPSC) en cholangiocytes fonctionnels. Ces cellules serviront à la modélisation des maladies génétiques touchant les cholangiocytes formant des canaux biliaires. Dans la deuxième partie, nous avons généré des iPSC spécifiques de patients HF (HF-iPSC), différenciées en hépatocytes et corrigé le défaut phénotypique par transfert lentiviral de l’ADNc codant le LDLR dans les HF-iPSC. / Cell therapy can be an alternative to liver transplantation in some cases such as severe metabolic diseases. However, the shortage of organ donors implies the need to find new sources of liver cells such as hepatocytes derived from pluripotent stem cells that can be amplified and differentiated extensively into any cell type. Human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) generated from somatic cells of patients and then differentiated into hepatocytes represent a potential source of transplantable hepatocytes. These cells now make it possible to consider the transplantation of genetically modified autologous hepatocytes as an alternative to liver transplantation for the treatment of genetic diseases of the liver.Familial hypercholesterolemia (FH) is an autosomal dominant disorder caused by mutations in the gene encoding the receptor for Low Density Lipoproteins (LDLR), which is the cause of high blood cholesterol in these patients. Homozygous patients should purify their serum LDL-apheresis on average twice a month starting at a young age to avoid fatal myocardial infarction occurring in childhood.Human hepatocytes differentiated from patient’s induced pluripotent stem cells (iPSCs) allow assessing the feasibility to transplant genetically modified autologous hepatocytes as treatment of familial hypercholesterolemia.During the liver development, hepatocytes and cholangiocytes, the two types of hepatic epithelial cells, derive from bipotent hepatic progenitors (hepatoblasts). Although cholangiocytes, forming intrahepatic bile ducts, represent a small fraction of the total liver cell population (3%), they actively regulate bile composition by secretion and reabsorption of bile acids, a process that is important in cholestatic liver diseases. In the first part of this study we developed an approach to differentiate pluripotent stem cells (hESC and hiPSC) into functional cholangiocytes. These cells could be used for the modeling of genetic biliary diseases. In the second part, we generated FH patient specific iPSCs (HF-iPSC), differentiated them into hepatocytes and tried to correct the disease phenotype by lentiviral introduction of LDLR cDNA cassette in HF-iPSC.

In the recent years, the exponential Internet traffic growth projections place enormous transmission rate demand on the underlying information infrastructure at every level, from the long haul submarine transmission to optical metro networks. In recent years, optical transmission at 100 Gb/s Ethernet date rate has been standardized by ITU-T and IEEE forums and 400Gb/s and 1Tb/s rates per DWDM channel systems has been under intensive investigation which are expected to be standardized within next couple of years.To facilitate the implementation of 400GbE and 1TbE technologies, the new advanced modulation scheme combined with advanced forward error correction code should be proposed. Instead of using traditional QAM, we prefer to use some other modulation techniques, which are more suitable for current coherent optical transmission systems and can also deal with the channel impairments. In this dissertation, we target at improving the channel capacity by designing the new modulation formats. For the first part of the dissertation, we first describe the optimal signal constellation design algorithm (OSCD), which is designed by placing constellation points onto a two dimensional space. Then, we expand the OSCD onto multidimensional space and design its corresponding mapping rule. At last, we also develop the OSCD algorithm for different channel scenario in order to make the constellation more tolerant to different channel impairments. We propose the LLR-OSCD for linear phase noise dominated channel and NL-OSCD for nonlinear phase noise dominated channel including both self-phase modulation (SPM) and cross-phase modulation (XPM) cases. For the second part of the dissertation, we target at probability shaping of the constellation sets (non-uniform signaling). In the conventional data transmission schemes, the probability of each point in a given constellation is transmitted equally likely and the number of constellation sets is set to 2!. If the points with low energy are transmitted with larger probability then the others with large energy, the non- uniform scheme can achieve higher energy efficiency. Meanwhile, this scheme may be more suitable for optical communication because the transmitted points with large probabilities, which have small energy, suffer less nonlinearity. Both the Monte Carlo simulations and experiment demonstration of both OSCD and non-uniform signaling schemes indicate that our proposed signal constellation significantly outperforms QAM, IPQ, and sphere-packing based signal constellations.

Thesis (MScEng (Electrical and Electronic Engineering))--University of Stellenbosch, 2009. / ENGLISH ABSTRACT: Low earth orbit (LEO) satellite communication presents a unique environment which inherently di ers from most other communication channels. Due to the varying orbital patterns of LEO satellites the link exhibits varying link margins. Limited communication time windows need to be optimised to maximise the volumetric data throughput. Large coding gains can be obtained by the implementation of forward error correction codes. This thesis presents a means for optimising the data throughput of LEO satellite communication through the implementation of a mission speci c error control strategy. Low density parity check (LDPC) codes are versatile and present good error performances at many di erent code rates and block lengths. With power limitations on the space segment and remote ground stations, hardware utilisation e ciency must be optimised to reduce power consumption. In response to this requirement, this thesis evaluates various algorithms for LDPC decoders. An iterative LDPC decoder, implementing an approximation algorithm, is presented as a low complexity solution with good error performance. The proposed solution provides a very good balance between required hardware complexity and coding performance. It was found that many parameters of the decoders and codes can be altered to allow the implementation of these codes in systems with varying memory and processing capabilities. / AFRIKAANSE OPSOMMING: Kommunikasiekanale van satelliete met lae wentelbane, bied 'n unieke omgewing wat inherent verskil van meeste ander kommunikasiekanale. As gevolg van veranderende wentelbaanpatrone, vertoon die kanaal 'n wisselende foutgedrag. Kommunikasievensters is beperk en moet geoptimeer word om die totale deurset van die stelsel te maksimeer. Groot koderingswinste kan verkry word deur die implementering van foutkorreksie kodes. Hierdie tesis voorsien 'n metode om die datadeurset van satelliete met lae wentelbaan te optimeer, deur middel van implementering van 'n missie-spesi eke foutbeheer strategie. Lae digtheid pariteit toetskodes (LDPC) is veelsydige kodes, bied goeie foutbeheer en is doeltre end vir verskillende kodekoerse en bloklengtes. Met drywingsbeperkinge op die ruimtesegment en afgesonderde grondstasies, moet hardeware komponente doeltreffend gebruik word om drywingsverbruik te verminder. Ten einde aan hierdie ontwerpsvereiste te voldoen, evalueer hierdie tesis verskeie LDPC dekodeerderalgoritmes. Deur 'n iteratiewe LDPC dekodeerder met 'n benaderingsalgoritme te implementeer, word 'n oplossing van lae kompleksiteit aangebied, maar wat steeds goeie foutkorreksie eienskappe toon. Die voorgestelde oplossing bied 'n baie goeie balans tussen benodigde hardeware kompleksiteit en koderingsprestasie. Daar is gevind dat heelwat parameters van die dekodeerders en kodes aangepas kan word, ten einde implementering in stelsels met 'n wye verskeidenheid van geheuespasie en verwerkingsvermoëns moontlik te maak.

Thesis (MScEng)--Stellenbosch University, 2012. / ENGLISH ABSTRACT: A collaboration between the Katholieke Universiteit van Leuven (KUL) and Stellenbosch University (SU), resulted in the development of a satellite based platform for use in agricultural sensing applications. This will primarily serve as a test platform for a digitally beam-steerable antenna array (SAA) that was developed by KUL. SU developed all flight - and ground station based hardware and software, enabling ground to flight communications and interfacing with the KUL SAA. Although most components had already been completed at the start of this M:Sc:Eng: project, final systems integration was still unfinished. Modules necessary for communication were also outstanding. This project implemented an automatic repeat and request (ARQ) strategy for reliable file transfer across the wireless link. Channel coding has also been implemented on a field programmable gate array (FPGA). This layer includes an advanced forward error correction (FEC) scheme i.e. a low-density parity-check (LDPC), which outperforms traditional FEC techniques. A flexible architecture for channel coding has been designed that allows speed and complexity trade-offs on the FPGA. All components have successfully been implemented, tested and integrated. Simulations of LDPC on the FPGA have been shown to provide excellent error correcting performance. The prototype has been completed and recently successfully demonstrated at KUL. Data has been reliably transferred between the satellite platform and a ground station, during this event. / AFRIKAANSE OPSOMMING: Tydens ’n samewerkingsooreenkoms tussen die Katholieke Universiteit van Leuven (KUL) en die Universiteit van Stellenbosch (US) is ’n satelliet stelsel ontwikkel vir sensor-netwerk toepassings in die landbou bedryf. Hierdie stelsel sal hoofsaaklik dien as ’n toetsmedium vir ’n digitaal stuurbare antenna (SAA) wat deur KUL ontwikkel is. Die US het alle hardeware en sagteware komponente ontwikkel om kommunikasie d.m.v die SAA tussen die satelliet en ’n grondstasie te bewerkstellig. Sedert die begin van hierdie M:Sc:Ing: projek was die meeste komponente alreeds ontwikkel en geïmplementeer, maar finale stelselsintegrasie moes nog voltooi word. Modules wat kommunikasie sou bewerkstellig was ook nog uistaande. Hierdie projek het ’n ARQ protokol geïmplementeer wat data betroubaar tussen die satelliet en ’n grondstasie kon oordra. Kanaalkodering is ook op ’n veld programmeerbare hekskikking (FPGA) geïmplementeer. ’n Gevorderde foutkorrigeringstelsel, naamlik ’n lae digtheids pariteit toetskode (LDPC), wat tradisionele foutkorrigeringstelsels se doeltreffendheid oortref, word op hierdie FPGA geïmplementeer. ’n Kanaalkoderingsargitektuur is ook ontwikkel om die verwerkingspoed van data en die hoeveelheid FPGA logika wat gebruik word, teenoor mekaar op te weeg. Alle komponente is suksesvol geïmplementeer, getoets en geïntegreer met die hele stelsel. Simulasies van LDPC op die FPGA het uistekende foutkorrigeringsresultate gelewer. ’n Werkende prototipe is onlangs voltooi en suksesvol gedemonstreer by KUL. Betroubare data oordrag tussen die satelliet en die grondstasie is tydens hierdie demonstrasie bevestig.

Coronary artery disease (CAD) is caused by atherosclerosis, a chronic inflammatory response to modified lipoproteins. A key pathophysiological event is the lipid-induced transformation of macrophages into lipid-laden foam cells and their accumulation in atherosclerotic plaques. Heritable CAD risk is associated with common genetic variants at over 40 genomic loci; the underlying causal mechanisms remain largely unknown and could affect transcriptional regulation in foam cells. Epigenetic and gene expression changes were measured in primary human macrophages before and after exposure to atherogenic, oxidized low-density lipoprotein—with resultant foam cell formation. This unbiased approach involved open chromatin mapping with formaldehyde-assisted isolation of regulatory elements with enhancer and transcription factor mapping using chromatin immuno-precipitation. Foam cell formation was associated with changes in a subset of open chromatin and enhancer sites that were strongly correlated with expression of nearby genes. OxLDL-regulated enhancers were enriched for several transcription factors—including C/EBP-beta— that have no previously documented role in foam cell formation. OxLDL exposure up-regulated C/EBP-beta expression and increased C/EBP-beta binding across the genome, most prominently around genes involved in inflammatory response pathways. Variants at CAD-associated loci were enriched in the subset of oxLDLregulated open chromatin sites. These included rs72664324 in an oxLDL-induced super-enhancer at the PPAP2B locus. OxLDL increased C/EBP-beta binding at rs72664324. C/EBP-beta binding, enhancer activity and oxLDL-induced upregulation of PPAP2B were stronger with the protective A allele of rs72664324. The PPAP2B protein product LPP3 was expressed in foam cells in human atherosclerotic plaques and was upregulated by oxLDL exposure in macrophages, so increasing the degradation of pro-inflammatory mediators. I also found several other CAD risk candidate genes were regulated by oxLDL: Phosphatase and actin regulator 1 (PHACTR1) and macrophage inducible Ca²⁺ dependent C-type lectin (Mincle). This led us to find a novel expression-quantitative-trait locus for PHACTR1 in macrophages and define new glycolipid ligands for Mincle. Our results demonstrate a genetic mechanism contributing to CAD risk at the PPAP2B locus and highlight the value of integrating gene expression and epigenetic changes to study disease processes involving pathogenic environmental stimuli.

Numerous chemical species are important to the health of biological systems. Some species can be beneficial at low doses and harmful at high doses. Other species are highly reactive and trigger serious cell damage. Improved methods to detect the presence and activity of such species are needed. In this work, several biologically important species were studied using appropriate analytical techniques. Fluoride is an important species in human physiology. It strengthens teeth and gives protection against dental caries. However, elevated concentrations of fluoride in the body can lead to health problems such as dental and skeletal fluorosis. Reported fluoride sensors used fluorescence quenching methods in determining fluoride concentration. Our study explored synthesis and characterization of 1,8-bis(phenylthioureido) naphthalene (compound 1) as a fluoride sensing molecule. Compound 1 showed a remarkable 40 fold enhancement in fluorescence with 5 eq of fluoride addition. Compound 1 also showed possibility of visual colorimetric sensing with fluoride. Free radical mediated oxidations of biomolecules are responsible for different pathological conditions in the human body. Superoxide is generated in cells and tissues during oxidative burst. Moderately reactive superoxide is converted to peroxyl, alkoxyl and hydroxyl radicals by various enzymatic, chemical, and biochemical processes. Hydroxyl radical imparts rapid, non specific oxidative damage to biomolecules such as proteins and lipids. Superoxide also reacts with nitric oxide in cells to yield peroxynitrite, which is highly reactive and damages biomolecules. Both hydroxyl radical and peroxynitrite readily react with amino acids containing aromatic side chains. Low density lipoprotein (LDL) carries cholesterol in the human body. Elevated concentration of LDL is a potential risk factor for atherosclerosis. Previous research drew a strong correlation between oxidized low density lipoprotein (ox-LDL) and plaque formation in the arterial wall. More importantly, oxidative damage causes structural changes to the LDL protein (apo B-100) which might facilitate the uptake of LDL by macrophages. In this study LDL was exposed to various concentrations of hydroxyl radical peroxynitrite and hypochlorite. Thereafter oxidized amino acid residues in apo B-100 were mapped by LC-MS/MS methods. We found widely distributed oxidative modifications in the apo B-100 amino acid sequence.

A madeira foi largamente utilizada em edificações no período colonial brasileiro por sua abundância em variadas espécies e a facilidade de obtenção e adaptação aos fins previstos. Muitos destes imóveis ainda existem e abrangem a maior parte do patrimônio histórico e cultural de nossas cidades. Devido à degradação causada pelo tempo e a falta de manutenção adequada destes elementos estruturais, as edificações estão expostas ao risco de colapso. O objetivo deste trabalho foi sugerir um método de reabilitação parcial de vigas estruturais de madeira, pois se notou um problema recorrente nestes elementos em construções antigas pelo fato de acumularem umidade, serem alvos de patologias principalmente na parte superior. Para reabilitação empregamos madeiras de baixa densidade pela facilidade de compra desse material, baixo custo e principalmente por aderir muito bem a tratamento preservativo com CCB, o que garante que a estrutura estará protegida de novos ataques. Além das madeiras de baixa densidade tratadas, parafusos auto atarraxantes foram escolhidos para fazer a união entre as madeiras. Para os ensaios de flexão estática de 3 pontos foram adotados 4 diferentes tipos de parafusos variando o diâmetro e comprimento além disso, o número de parafusos inseridos nos corpos de prova também variou. Este estudo contém a análise de relação entre a quantidade de parafusos auto atarraxantes (que fazem a união das peças) e o produto de rigidez de peças mistas compostas de madeira referência (que simula a viga em estado de degradação) e madeira tratada (substituindo a porção degradada). Para a madeira referencia adotou-se Eucalipto (classe C30 de resistência) e Roxinho (classe C60) e, para madeira tratada adotou-se Pinus e Caxeta (de baixa densidade, ambas da classe C20). Análises estatísticas foram utilizadas para interpretação dos resultados obtidos em laboratório. Estas análises apontaram o crescimento do produto de rigidez das vigas mistas conforme a inserção de parafusos e a partir dai foi possível expressar em formulas a correlação dos dados. Utilizou-se o teste de Tukey para apontar o melhor tratamento para cada tipo de viga mista (Pinus e Eucalipto, Roxinho e Caxeta) além do melhor parafuso a se utilizar. / Wood was widely used in buildings in the Brazilian colonial period because of it is abundant in varied species and the ease of obtaining and adapting to intended purposes. Many of these properties still exist and cover most of historical and cultural heritage of Brazilian cities. Due to the degradation caused by the weather and lack of adequate maintenance of these structural elements, buildings are exposed to risk of collapse. The objective of this work was to suggest a method of partial rehabilitation of structural timber beams, since a recurrent problem was observed in these elements in old constructions due to accumulation of humidity, being targets of pathologies mainly in their upper part. For rehabilitation we use low density wood because of ease to purchasing this material, low cost and mainly for adhering very well to condom treatment with CCB, which ensures that the structure will be protected from further attacks. In addition to the treated low density woods, self-tapping screws were chosen to make union between woods. For tests, 4 different types of screws were used, varying diameter and length, and number of screws inserted in the specimens also varied. This study contains an analysis of relationship between the number of self-tapping screws (which make the joining of pieces) and the rigidity product of composite pieces of reference wood (which simulates beam in degraded state) and treated wood (replacing degraded portion). Eucalyptus (class C30 of resistance) and Roxinho (class C60) were used for reference wood, and Pinus and Caxeta (low density, both of class C20) were used for treated wood. Statistical analyzes were used to interpret results obtained in laboratory. These analyzes indicated growth of product of flexural stiffness according to insertion of screws and from this it was possible to express in formulas a correlation of data. Tukey test was used to indicate the best treatment for each type of mixed beam (Pinus and Eucalyptus, Roxinho and Caxeta) as well as the best screw to be used.