This diploma thesis deals with hot-formed helical compression springs. The first part of the thesis describes related problems and determines market potential. Following theoretical part describes the influence of contact line on behaviour of springs and compares it with normative regulations. Methods suitable for determination of helix shape are covered in the next part. The last part focuses on practical measurement, in which the change of helix shape during manufacture was observed. The main output of this diplomat thesis is suggestion of measures that should contribute to both optimization of manufacture and verification of FEM model.

Le TNF-α est une cytokine pro-inflammatoire du système immunitaire qui, lorsque sa production est déréglée, induit de nombreuses pathologies chez l’homme (cachexie, arthrite rhumatoïde, etc.) Outre une régulation transcriptionnelle de cette cytokine, il existe aussi une régulation post-transcriptionnelle permettant un contrôle affiné de sa production. Le laboratoire de Biologie du Gène étudie cette régulation post-transcriptionnelle faisant intervenir une séquence consensus dans l’ARNm appelée séquence AU-riche (ou ARE pour AU-rich element) et les protéines qui y sont impliquées. Généralement, les ARNm porteurs d’ARE codent pour des protéines dont l’expression est transitoire. Ces gènes requièrent un contrôle très précis de leur expression et c’est pourquoi, en plus d’être soumis à de nombreux contrôles transcriptionnels, la traduction et la stabilité de leurs ARNm sont très finement régulées. La réponse immune innée implique de nombreux ARNm de ce type. Jusqu’à présent, la fonction de la protéine TIAR dans la régulation de l’expression du TNF-α n’a pas été complètement élucidée. Outre le TNF-α, la participation à la réponse immune innée de nombreuses protéines encodées par des ARNm porteurs d’ARE pourrait conférer à la protéine TIAR un rôle de régulateur essentiel dans le contrôle de l’inflammation. Nous avons donc générés plusieurs lignées de macrophages RAW 264.7 surexprimant la protéine TIAR entière ou différents mutants de TIAR afin de déterminer, par une analyse globale par puces à ADN, les ARNm cibles de TIAR au cours de la réponse immune. Cette approche nous a permis de démontrer que la protéine TIAR exerce un contrôle sur le métabolisme de l’ARNm du TNF-α et de MKP-1 (MAP kinase phosphatase 1), une phosphatase majeure dans la voie de signalisation de la MAPK p38. Cette voie de signalisation joue un rôle essentiel dans la stabilisation et la traduction de nombreux ARNm porteurs d’ARE encodant des protéines de la réponse inflammatoire. D’autre part, nous avons voulu étudier in vivo la fonction de la protéine TIAR au cours de la réponse immune. Nous avons, dans ce but, généré des souris transgéniques surexprimant l’isoforme courte de la protéine TIAR. Si nous n’avons pas encore pu mesurer les effets d’une surexpression de TIAR sur la réponse inflammatoire chez ces souris, ces individus transgéniques ont révélé qu’une expression anormale de la protéine TIAR induit une létalité importante au cours du développement embryonnaire. / Doctorat en Sciences / info:eu-repo/semantics/nonPublished

The thesis solves mounting of Continental IO-55ON to four-seat all composite aeroplane. Weight analysis of weights carried by engine mounting was done and loads of mounting and nose gear attached directly to mounting was computed according to CS-23 regulation. Mounting was designed based on affordable information about similar aeroplanes and it was sized due to Nastran/Patran system strength analysis. Further reinforcing of fire wall, engine covers and forms for their manufacture was suggested.

The aim of this thesis is to description and comparison of solutions, demountable connection of the lid and box enclosure air filters for motor vehicles, developed and used by the manufacturing concern MANN + HUMMEL, for which air filtration only one part of a broad portfolio of filtration systems for the automotive industry in the world scale. Another part of this thesis will be one of the chosen optimization methods of connection and implementation of alternative proposals design solutions, including the selection of the best solution, creating design calculation, 3D design, FEA analysis and prototyping of an alternative connection method laser sintering. When creating the optimization and design of alternative design solutions can count on the possibility that existing solutions are taking all possible factors and that the best alternative design proposals show an improvement over existing at the expense of all other cells throughout the development of the product.

Thesis deals with determination of layerwise mechanical properties of composite ceramics by indirect method, namely Youngs modulus. Based on literature review, it was found that a method to determine elastic properties of one or more components of multi layered composites based on experimental modal analysis and finite element modal analysis, or analytical approach exists. Method based on FE modal analysis was applied to ceramic laminate, where it was attempt to determine youngs modulus of one component. Beyond that, it was attempt to determine Youngs moduli of both components using first two bending resonant frequencies. Results were unsatisfying. Sensitivity analysis showed that layers with unknown Youngs modulus were overly sensitive to small changes in input parameters due to their small relative thickness with respect to thickness of laminate and due to location in laminate. Based on this conclusion, recommendations were made with respect to suitable geometry of test specimens.

This master thesis deals with computational stress-strain analysis of the tailskid of airplane L410 NG with main focus firstly the check current design of the tailskid and then the design another design solution with the able to absorb as much as possible the deformation energy. Solution of this problem is performed using computational modeling utilizing numerical simulation of quasi-static and crash deformation load of the tailskid with using explicit Finite Element Method (FEM) in program ABAQUS v6.14. After the introduction with problem situation and tailskid assembly introductory part is devoted to the research study of various designs of the tailskid for different types of airplanes. There follows these theoretical general principles of thin-walled structures and buckling of them. Before the creating of the computational model itself, the explicit form of the Finite Element Method is better described. The conclusion of this thesis deals with the mutual comparison of the most advantageous design variants of the tailskid and the selection of the most suitable one of them for the airplane L410 NG.

This diploma thesis focuses on strength design of steam condenser. The goal of the thesis is to make strength calculations for the specific operation conditions, introduce possible solutions, provide recommendations and refer to weak points of such calculation procedures. First, thermal-hydraulic design in HTRI software is performed. Strength calculations respect ČSN EN 13445 standard. Strength calculation with imported temperature field is performed in ANSYS Workbench software. In the next step, another strength calculation is realized in Sant´ Ambrogio software. Results are evaluated in conclusion chapter, including recommendations for the possible following work.

The aim of this diploma thesis is the design of the configuration and the method of balancing the crank mechanism with the specified parameters. For a particular configuration, a crankshaft drawing is processed and a strength analysis, considering the crankshaft torsional vibrations is performed in Ansys FEM software. At the end of the thesis, an ideological design of the torsional vibration damper and its effect on the crankshaft stress in the Ansys FEM software is performed.

The objective of this diploma thesis is the design of a device that measures the vehicle’s centre of gravity height position. The first part defines individual methods of measurement using various measuring equipment. The next part deals with the definition of requirements for the device and the definition of the design. A stress-strain analysis was executed for selected components. This diploma thesis describes the preparation process of the vehicle and the device for the measurement. The next part is dedicated to the design of the measuring chain and to the evaluation of the results. The thesis concludes with a theoretic analysis of the measurement inaccuracy.

Csaba CSEH Engine Connecting Rod Analysis Including Bolts Influence DW, ITE, 2008, 75 pp, 88 fig. The aim of my diploma work is a stress analysis in the material of a connecting rod during a stationary engine regime. Dynamic forces from the connecting rod‘s motion and the forces acting on the bolts are included in the calculations. The whole stress analysis is based on a FEM designed application on a model, that was created in a CAD system package according to the real component.