Spelling suggestions: "subject:"micelles."" "subject:"bicelles.""
301 |
Mechanistic studies to evaluate the targeting specificity of novel RGD Micelles to the αVβ3 integrin receptorRaj, April 01 January 2012 (has links)
Current chemotherapeutics pose many di sadvantages due to their lack of specificity and low therapeutic index. To overcome these challenges, research has focused its attention on the development of nano-based delivery systems that can penetrate the leaky vasculature of tumor endothelium, use site-directed ligands that can bind with high affinity and specific ity to tumor cells, physically entrap poorly soluble drugs, and deliver these cytotoxic agents directly to the tumor site. One approach to nanosystem drug delivery is with the use of peptide amphiphiles (PAs) that are conjugated with the Arginine-Glycine-Aspartic Acid (RGD) motif to actively target a αVβ3 integrin receptors on cancer cells or tumor endothelium. The current work is focused on mechanistic studies to evaluate the uptake of novel RGD amphiphi les with varying alkyl chain lengths (palmitic acid : Cl 6 and stearic acid: C 18) and hydrophilic linkers, 8-amino- 3,6-dioxaoctonoic acid (ADA) or glucose, as micellar delivery systems of hydrophobic anticancer agents. PAs were confirmed for their self-assembling properties and further evaluated for their RGD-mediated binding specificity to purified αVβ3 integrin through a competitive binding fluorescence polarization assay (with novel RGD micelles displacing an integrin-bound fluorescent RGD probe by as much as 63.03%). Ultimately, these nanocarriers were assessed for their ability to deliver phys ically entrapped fluorescein isoth iocyanate (FITC) to A2058 cells overexpressing αVβ3 integrin receptors. Results from confocal microscopy indicate that uptake of RGD micelles was driven by an energy-dependent mechanism, as statistically significant levels of FITC internalization was seen at 37°C versus 4°C (p-value<0.05 for all treatment groups); moreover, intracellular fluorescence was notably higher (as much as 4-fold) when delivered through novel RGD conjugates as opposed to its free form. Regardless of chain length and the number of hydrophilic linkers, all RGD PAs showed promising results as micellar carriers that can effectively deliver their payload to the target tumor site via receptor mediated endocytosis.
|
302 |
Development and Characterization of LDV Peptide Targeted Nanocarriers for Paclitaxel Delivery: A Comparative Study of Micelles, Liposomes and Solid Lipid NanoparticlesDattani, Poonam 01 January 2019 (has links)
Nanocarriers have been established as delivery vehicles to target cancer tumors. However, premature drug leakage is one of the major reasons for inefficient drug delivery of nanocarriers to the tumor. Drug diffusion out of the nanocarriers or destabilization of drug loaded nanocarriers by physiological interactions with blood cells, serum proteins, and cell membranes upon systemic administration contribute to premature drug release. In this study, targeted micelles, liposomes and solid lipid nanoparticles (SLNs) of similar composition were prepared and characterized to compare physicochemical characteristics, in vitro stability, in vitro release rates in release media and in vivo performance. Peptide Amphiphiles (PAs) formed micelles with critical micelle concentration (CMC) values ranging between 23.68 ± 0.72 µM to 38.76 ± 2.27 µM. Transmission Electron Microscopy (TEM) images confirmed the self-assembly of PAs into spherical structures where the largest sizes were seen for C16-(PEG2)6-LDV micelles. Dynamic Light Scattering (DLS) results confirmed the presence of targeted liposomes and SLNs with sizes smaller than 100 nm. Forster Resonance Energy Transfer (FRET) studies revealed that targeted micelles, liposomes and SLNs were all stable upon dilution in aqueous medium, however the stability was significantly reduced in human serum, with micelles being the least stable and SLNs being the most stable. The same trend was observed for the in vitro release profiles, where targeted paclitaxel-loaded micelles (PTX-micelles) had the fastest release rate and paclitaxel-loaded SLNs (PTX-SLN) exhibited the slowest release rate. DLS results showed that sizes of PTX-SLNs were smaller than PTX-liposomes (80.53 ± 5.37 nm vs 123.31 ± 5.87 nm). Cryogenic TEM observation showed increasing size in the order of PTX-micelles (6 to 12 nm) < PTX-SLNs (10-120 nm) < PTX-liposomes (48-145 nm). Drug Loading Content (DLC) of PTX-SLNs was greater than PTX-micelles and PTX-liposomes (7.45 ± 0.41 % vs 1.70 ± 0.42 % and 0.92 ± 0.09 %). Compared to initial aqueous dispersions, reconstituted spray dried formulations maintained their nanosize and paclitaxel content over 7 days at 4⁰C. In A375 melanoma xenograft mouse model, the tumor volumes were significantly smaller for mice treated with PTX-SLNs compared to the control group. Furthermore, tumor volumes were significantly smaller for mice treated with PTX-SLNs compared to those treated with PTX-micelles and PTX-liposomes. These studies demonstrate the potential of stable PTX-SLNs for targeted delivery in cancer.
|
303 |
Microgels as drug carriers : Relationship between release kinetics and self-aggregation of the amphiphilic drugs adiphenine, pavatrine and diphenhydramine.Ali Mohsen, Lobna January 2021 (has links)
Abstract There has been great interest in microgels as drug carriers within the pharmaceutical industry. This includes the use of amphiphilic drugs for treating conditions such as depression, allergies, and cancer. By loading adiphenine (ADP), pavatrine (PVT), and diphenhydramine (DPH) into macrogels and observing the release, this study seeks to investigate how amphiphilic drugs can be released from microgels. There is also an interest in how aggregation behavior may vary depending on the structural components. This study utilized small angle x-ray scattering (SAXS) along with UV analysis and the measuring of the binding isotherm to investigate micelle aggregation and aggregation number. Two of the drugs adiphenine and pavatrine, have similar structures with only one bond that differentiated them. The difference in rigidity provided different results in SAXS. Adiphenine has an aggregation number of 12, diphenhydramine has a number of 13, and pavatrine has a number of 37. In contrast to pavatrine, which did not exhibit a correlation peak, adiphenine and diphenhydramine showed correlation peaks. This indicates that none of them had an ordered phase structure but pavatrine displayed an even more disordered phase structure. Nevertheless, all three drugs were in equilibrium, and so a difference between adiphenine and pavatrine could be clearly distinguished. There were significant divergences between pavatrine and adiphenine despite not being able to determine binding isotherms for all three drugs. Based on this, they should be less stable than diphenhydramine. They have an ester linkage, while diphenhydramine doesn't. As a result, it was not possible to confirm how self-aggregation of adiphenine, pavatrine, and diphenhydramine impacts drug release. Despite this, differences in the rigidity of the structural form may lead amphiphilic drugs to exhibit different behaviour in gels. Keywords: Amphiphilic drugs, small angle x-ray scattering, macrogels, binding isotherm, CMC, self-aggregation, phase structure, micelles.
|
304 |
Molecular Dynamics Simulations of Polymers and Micelles at InterfacesSeverin, Nikolai 08 July 1999 (has links)
Molekulardynamik (MD) Simulationen wurden an zwei verschiedenen Systemen durchgeführt: 1. Grenzfläche zwischen Polyethylen und isotaktischem Polypropylen (PE-iPP) und 2. Zylindrische Mizellen, bestehend aus Tetradecyltrimethylammoniumbromid (C14TAB), in wässriger Lösung und an Fest-Flüssig-Grenzflächen. Die allgemeinen Schwierigkeiten bei der Simulation von Grenzflächen kristalliner Polymere wurden diskutiert und eine Methode für solche Simulationen vorgeschlagen. Diese Methode wurde zur epitaxialen Kristallisation von PE auf iPP benutzt. Experimentelle Ergebnisse der epitaxialen Kristallisation konnten durch die Simulation bestätigt werden. Ferner konnte vorhergesagt werden, dass PE bevorzugt auf einer iPP-Oberfläche mit hoher Methylgruppenkonzentration kristallisiert. Ebenso wurde durch die MD Simulation vorhergesagt, dass PE in der Grenzflächenregion von einer orthorhombischen zur monoklinischen Kristallstruktur wechselt. Die Simulationsdauer für die Mizellen betrug einige Nanosekunden. Die Ergebnisse für die Mizellen in wässriger Lösung stehen hierbei in guter Übereinstimmung mit experimentellen Werten. Im Widerspruch zur allgemein üblichen Vorstellung führte die Simulation der Mizellen zur Ausbildung eines Hohlraums in ihrer Mitte sowie zu einer inhomogenen Dichte des hydrophoben Mizellkerns. Dies wurde zum Teil der inhomogenen Verteilung der terminalen Methylgruppen im Mizellkern zugeschrieben. Zylindrische und halbzylindrische Mizellen wurden an den Paraffin/Wasser- und Gold/Wasser-Grenzflächen simuliert. / Molecular Dynamic (MD) simulation of two different systems was performed: 1) Polyethylene- isotactic Polypropylene (PE-iPP) interfaces and 2) cylindrical micelles formed by tetradecyl trimethylammonium bromide (C14TAB) molecules in aqueous solution and at solid liquid interfaces. The general difficulties of simulation of polymer crystalline interfaces were discussed and one method was proposed for such simulations. Thise method was used to simulate epitaxial crystallisation of PE on iPP. The experimental results on epitaxial crystallisation were confirmed by MD simulation and in addition epitaxial crystallisation of PE on iPP surface with high dencity of methyl groups was predicted. MD simulation also predicted that PE should change at the interfacial region from the orthorhombic to monoclinic crystalline structure. Several nanoseconds of life of cylindrical micelles were simulated. The simulation results for the micelle in aqueous solution were favourably compared with experimental results. In contradiction to the standard picture of an ionic micelle the simulated micelle formed hole in its centre and the density of the hydrophobic micelle core was inhomogeneous. This effect partially was explained by the inhomogeneous distribution of the terminal methyl groups in the micelle core. Cylindrical and half cylindrical micelles of C14TAB molecules were simulated at the paraffin- and gold-aqueous interfaces.
|
305 |
DYNAMICS OF POLYMER SELF-ASSEMBLY BY COMPUTER SIMULATIONLI, ZHENLONG 21 March 2011 (has links)
No description available.
|
306 |
Correlations among surfactant drag reduction additive chemical structures, rheological properties and microstructures in water and water/co-solvent systemsZhang, Ying 12 September 2005 (has links)
No description available.
|
307 |
Membrane binding properties of Disabled-2Alajlouni, Ruba 10 May 2011 (has links)
Disabled-2 (Dab2) is an adapter protein that interacts with cell membranes and it is involved in several biological processes including endocytosis and platelet aggregation. During endocytosis, the Dab2 phosphotyrosine-binding (PTB) domain mediates protein binding to phosphatidylinositol 4,5-bisphosphate (PIP2) at the inner leaflet of the plasma membrane and helps co-localization with clathrin coats. Dab2, released from platelet alpha granules, inhibits platelet aggregation by binding to the °IIb? integrin receptor on the platelet surface through an Arg-Gly-Asp (RGD) motif located within the PTB domain. Alternatively, Dab2 binds sulfatides on the platelets surface, and this binding partition Dab2 in two pools (sulfatide and integrin receptor-bound states), but the biological consequences of lipid binding remain unclear. Dab2 binds sulfatides through two basic motifs located on its N-terminal region including the PTB domain (N-PTB). We have characterized the binding of Dab2 to micelles, which are widely used to mimic biological membranes. These micellar interactions were studied in the absence and presence of Dab2 lipid ligands, sulfatides and PIP2. By applying multiple biochemical, biophysical, and structural techniques, we found that whereas Dab2 N-PTB binding to PIP2 stabilized the protein but did not contribute to the penetration of the protein into micelles, sulfatides induced conformational changes and facilitated penetration of Dab2 N-PTB into micelles. This is in agreement with previous observation that sulfatides, but not PIP2, protect Dab2 N-PTB from thrombin cleavage. By studying the mechanism by which Dab2 targets membranes, we will have the opportunity to manipulate its function in different lipid-dependent biological processes. / Master of Science
|
308 |
Cyclic graft copolymer unimolecular micelles : effects of cyclization on particle morphology and thermoresponsive behaviorWilliams, R.J., Pitto-Barry, Anaïs, Kirby, N., Dove, A.P., O'Reilly, R.K. 2016 March 1917 (has links)
Yes / The synthesis of cyclic amphiphilic graft copolymers with a hydrophobic polycarbonate backbone and hydrophilic poly(N-acryloylmorpholine) (PNAM) side arms via a combination of ring-opening polymerization (ROP), cyclization via copper-catalyzed azide–alkyne cycloaddition (CuAAC), and reversible addition–fragmentation chain transfer (RAFT) polymerization is reported. The ability of these cyclic graft copolymers to form unimolecular micelles in water is explored using a combination of light scattering, small-angle X-ray scattering (SAXS), and cryogenic transmission electron microscopy (cryoTEM) analyses, where particle size was found to increase with increasing PNAM arm length. Further analysis revealed differences in the solution conformations, loading capabilities, and morphologies of the cyclic graft copolymers in comparison to equivalent linear graft copolymer unimolecular micelle analogues. Furthermore, the cyclic and linear graft copolymers were found to exhibit significantly different cloud point temperatures. This study highlights how subtle changes in polymer architecture (linear graft copolymer versus cyclic graft copolymer) can dramatically influence a polymer’s nanostructure and its properties. / Royal Society (Great Britain), Engineering and Physical Sciences Research Council (EPSRC), European Research Council (ERC)
|
309 |
Nouveaux marqueurs pour l'observation du moteur flagellaire bactérienTruchon, Dany 18 April 2018 (has links)
De nombreuses bactéries se déplacent en faisant tourner des flagelles rigides ancrés dans leur membrane. À la base de chaque flagelle se trouve un moteur rotatif de quelques dizaines de nanometres de diamètre : le moteur flagellaire bactérien. L'objectif de ces travaux de maîtrise fut de développer de nouveaux marqueurs pour visualiser et mesurer la rotation du moteur flagellaire en l'affectant le moins possible. Trois types de nanoparticules furent étudiés : les points quantiques, les nanoparticules d'or et les nanoparticules Janus. L'intensité du signal et la résistance au photoblanchiment des points quantiques furent comparées à celles de fluorophores "Alexa Fluor". Aussi, différentes méthodes pour attacher spécifiquement ces nanoparticules aux flagelles furent testées. L'utilisation d'anticorps s'est révélée préférable à l'emploi de micelles de phospholipides ou de liens maléimide-cystéine. Après avoir développé la méthode de marquage sur les filaments des bactéries, nous avons ensuite réussi à visualiser des crochets, une structure beaucoup plus petite (55 nm de long). Une première tentative pour mesurer la vitesse de rotation de crochets s'est révélée infructueuse mais informative.
|
310 |
Characterization of the Interactions of the Bacterial Cell Division Regulator MinEHafizi, Fatima 23 August 2012 (has links)
Symmetric cell division in gram-negative bacteria is essential for generating two equal-sized daughter cells, each containing cellular material crucial for growth and future replication. The Min system, comprised of proteins MinC, MinD and MinE, is particularly important for this process since its deletion leads to minicells incapable of further replication. This thesis focuses on the interactions involving MinE that are important for allowing cell division at the mid-cell and for directing the dynamic localization of MinD that is observed in vivo. Previous experiments have shown that the MinE protein contains an N-terminal region that is required to stimulate MinD-catalyzed ATP hydrolysis in the Min protein interaction cycle. However, MinD-binding residues in MinE identified by in vitro MinD ATPase assays were subsequently found to be buried in the hydrophobic dimeric interface in the MinE structure, raising the possibility that these residues are not directly involved in the interaction. To address this issue, the ability of N-terminal MinE peptides to stimulate MinD activity was studied to determine the role of these residues in MinD activation. Our results implied that MinE likely undergoes a change in conformation or oligomerization state before binding MinD. In addition we performed circular dichroism spectroscopy of MinE. The data suggest that direct interactions between MinE and the lipid membrane can lead to conformational changes in MinE. Using NMR spectroscopy in an attempt to observe this structure change, different membrane-mimetic environments were tested. However the results strongly suggest that structural studies on the membrane-bound state of MinE will pose significant challenges. Taken together, the results in this thesis open the door for further exploration of the interactions involving MinE in order to gain a better understanding of the dynamic localization patterns formed by these proteins in vivo.
|
Page generated in 0.0445 seconds