This study concerns the identification of the particular competences required by education, training and development practitioners (ETD practitioners) in the South African National Defence Force to develop suitable and appropriate career and training strategies. An applied research approach and a primarily quantitative approach were used. Questionnaires were completed by the commanding officers or the training managers, as well as the ETD practitioners at the education, training and development providers in the South African National Defence Force to determine the actual utilisation of ETD practitioners. Descriptive statistics were used to determine the roles, core competences, levels of competences and clusters of competences required by ETD practitioners in the South African National Defence Force. In addition, the actual utilisation of ETD practitioners was compared with a proposed competence profile that was based on the literature study in order to determine the competence gap that has to be addressed by means of career and training strategies. / Educational Studies / M.Ed.(Didactics)

PURPOSE: The main aim of this study was to systematically assess women’s maternal health care seeking behaviour and its determinants in Addis Ababa, Ethiopia. DESIGN: A quantitative and cross-sectional community based study was the selected methodology for this study. METHOD: Data was collected using structured questionnaire administered to 903 women aged 15-49 years through a stratified two-stage cluster sampling technique. Binary and multinomial logistic regression models were employed to identify predictors of adequacy of antenatal care and delivery care. RESULTS: Most of the women (97.9%) visited health care facilities at least once for antenatal care follow up. About 86.5% of them had at least four visits during their last pregnancy; and only 51.1% started their first antenatal visit early. Further, only about one out of five of the antenatal care attendees received sufficient content of antenatal care services. Consequently, only about one out of ten women received overall adequate antenatal care mainly due to inadequate use of the basic components of antenatal services. Most of the women delivered in public health care institutions (76.3%) despite the general doubts about the quality of services in these facilities. Women of better socioeconomic status preferred to give birth at private health care facilities. Caesarean section delivery rate in Addis Ababa (19.1%) is higher than the maximum WHO recommended rate (15.0%); particularly among the non-slum residents (27.2%); clients of private health care facilities (41.1%); currently married women (20.6%); women with secondary (22.2%) and tertiary (33.6%) level of education; and women who belong to the highest wealth quintile (28.2%). The majority (65.8%) of the caesarean section clients were not informed about the consequences of caesarean section delivery and about 9.0% of the caesarean section births had no medical indication. CONCLUSION: Disparities in maternal health care utilisation between the socio-economic groups was evident, requiring urgent attention from policy makers and other stakeholders to enable Ethiopia to meet its millennium development goal 5. Improving the quality of antenatal care in public health facilities which are the main provider of health care services to the majority of the Ethiopian population is urgent. The increase in the rate of caesarean section beyond the World Health Organization recommended upper limit has to be taken seriously. / Health Studies / D. Litt.. et Phil. (Health Studies)

PURPOSE: The main aim of this study was to systematically assess women’s maternal health care seeking behaviour and its determinants in Addis Ababa, Ethiopia. DESIGN: A quantitative and cross-sectional community based study was the selected methodology for this study. METHOD: Data was collected using structured questionnaire administered to 903 women aged 15-49 years through a stratified two-stage cluster sampling technique. Binary and multinomial logistic regression models were employed to identify predictors of adequacy of antenatal care and delivery care. RESULTS: Most of the women (97.9%) visited health care facilities at least once for antenatal care follow up. About 86.5% of them had at least four visits during their last pregnancy; and only 51.1% started their first antenatal visit early. Further, only about one out of five of the antenatal care attendees received sufficient content of antenatal care services. Consequently, only about one out of ten women received overall adequate antenatal care mainly due to inadequate use of the basic components of antenatal services. Most of the women delivered in public health care institutions (76.3%) despite the general doubts about the quality of services in these facilities. Women of better socioeconomic status preferred to give birth at private health care facilities. Caesarean section delivery rate in Addis Ababa (19.1%) is higher than the maximum WHO recommended rate (15.0%); particularly among the non-slum residents (27.2%); clients of private health care facilities (41.1%); currently married women (20.6%); women with secondary (22.2%) and tertiary (33.6%) level of education; and women who belong to the highest wealth quintile (28.2%). The majority (65.8%) of the caesarean section clients were not informed about the consequences of caesarean section delivery and about 9.0% of the caesarean section births had no medical indication. CONCLUSION: Disparities in maternal health care utilisation between the socio-economic groups was evident, requiring urgent attention from policy makers and other stakeholders to enable Ethiopia to meet its millennium development goal 5. Improving the quality of antenatal care in public health facilities which are the main provider of health care services to the majority of the Ethiopian population is urgent. The increase in the rate of caesarean section beyond the World Health Organization recommended upper limit has to be taken seriously. / Health Studies / D. Litt. et Phil. (Health Studies)

This study concerns the identification of the particular competences required by education, training and development practitioners (ETD practitioners) in the South African National Defence Force to develop suitable and appropriate career and training strategies. An applied research approach and a primarily quantitative approach were used. Questionnaires were completed by the commanding officers or the training managers, as well as the ETD practitioners at the education, training and development providers in the South African National Defence Force to determine the actual utilisation of ETD practitioners. Descriptive statistics were used to determine the roles, core competences, levels of competences and clusters of competences required by ETD practitioners in the South African National Defence Force. In addition, the actual utilisation of ETD practitioners was compared with a proposed competence profile that was based on the literature study in order to determine the competence gap that has to be addressed by means of career and training strategies. / Educational Studies / M.Ed.(Didactics)

[ES] Antecedentes: Los patrones de colonización microbiana alterados durante la infancia pueden ser en parte responsables del aumento de enfermedades alérgicas en los países desarrollados. La microbiota intestinal difiere en composición y diversidad durante los primeros meses de vida en niños que luego desarrollan o no una enfermedad alérgica. Sin embargo, poco se sabe sobre la importancia de las respuestas inmunitarias tempranas de la mucosa a la microbiota intestinal en el desarrollo de alergias infantiles. Además, los estudios con respecto al efecto protector de la microbiota de la leche materna en el riesgo de desarrollar alergias no han sido concluyentes. Aunque la cavidad bucal es el primer lugar de encuentro entre la mayoría de los antígenos exógenos y el sistema inmunológico, no existen datos sobre la influencia de las bacterias orales en el desarrollo de alergias durante la infancia. Objetivos: El objetivo general de esta tesis fue evaluar la composición y diversidad microbiana en muestras orales, intestinales y de leche materna, junto con su interacción con IgA, para estudiar el papel de la colonización microbiana durante edades tempranas de la vida en condiciones de salud y de enfermedad alérgica. Sujetos: Los bebés y las madres incluidas en este estudio forman parte del ensayo aleatorio doble ciego más grande de Suecia, entre 2001 y 2003, donde se evaluaron los posibles efectos preventivos sobre la alergia de Lactobacillus reuteri ATCC 55730 hasta los 2 y 7 años. En esta tesis, utilizamos muestras de heces recogidas a los 1 y 12 meses, y muestras orales de bebés, obtenidas longitudinalmente a los 3, 6, 12, 24 meses y 7 años. Además, analizamos muestras de leche materna, recogidas a un mes después del parto de las madres correspondientes. Métodos: Se utilizaron tecnologías de secuenciación de segunda generación dirigidas al gen 16S rARN, en combinación con citometría de células marcadas por fluorescencia, para abordar las respuestas de IgA de la mucosa hacia las bacterias intestinales y de la leche materna. Además, se utilizó la secuenciación del gen 16S para describir la colonización oral de la microbiota, en muestras de saliva, de niños que desarrollaron alergias o de aquellos que se mantuvieron sanos. Los niveles de carga bacteriana en diferentes hábitats microbianos se obtuvieron mediante la metodología de qPCR y los niveles totales de IgA de las muestras de heces se determinaron mediante inmuno-ensayo ELISA. Resultados y conclusión: La colonización de la cavidad bucal durante la infancia temprana es progresiva, aumenta en complejidad con el tiempo, y varios factores externos parecen influir en gran medida en la maduración de la microbiota oral, ya sea con un impacto a corto o largo plazo. Los cambios tempranos en la composición microbiana oral parecen influir en la maduración inmune y el desarrollo de alergias en la infancia, y la presencia de especies bacterianas específicas puede ser importante para este proceso. Además, las respuestas de IgA alteradas hacia la microbiota intestinal durante la infancia precedieron a las manifestaciones de asma y alergia durante los primeros 7 años de vida, y el consumo de leche materna con una riqueza microbiana reducida en el primer mes de vida puede aumentar el riesgo de desarrollar alergia durante la infancia. Los hallazgos observados en la presente tesis deben confirmarse en cohortes más grandes y la importancia de los factores ambientales postnatales para el desarrollo temprano de la microbiota debe abordarse más a fondo. Las investigaciones futuras deben ir más allá de la caracterización de la composición de la comunidad bacteriana e investigar los mecanismos funcionales entre los microorganismos colonizadores tempranos, la maduración inmunitaria y la alergia, así como el desarrollo del asma durante la infancia. / [CAT] Antecedents: S'ha proposat que els patrons de colonització microbiana alterats durant la infància podrien ser en part els responsables de l'augment de malalties al·lèrgiques als països desenvolupats. La microbiota intestinal difereix en composició i diversitat durant els primers mesos de vida en els nens que després van desenvolupar una malaltia al·lèrgica. No obstant això, poc es sap sobre la importància de les respostes immunes de la mucosa a la microbiota intestinal en el desenvolupament d'al·lèrgies infantils. A més, les investigacions amb relació a l'efecte protector de la microbiota de la llet materna en el risc de desenvolupar al·lèrgies no han sigut concloents. Encara que la cavitat bucal és el primer lloc de trobada entre la majoria dels gèneres externs i el sistema immunològic, encara no s'ha descobert la influència dels bacteris en el desenvolupament d'una al·lèrgia durant la infància. Objectius: L'objectiu general d'aquesta tesi va ser avaluar la composició microbiana i la diversitat de mostres orals, fecals i llet materns, juntament amb la seva interacció amb IgA, per estudiar el paper del desenvolupament microbià durant el període de la infància primerenca a la salut i la malaltia al·lèrgica. Subjectes: Les mares i xiquets inclosos en aquest estudi formen part d'un estudi aleatori doble-cec a Suècia, entre el 2001 i el 2003, on es van avaluar els possibles efectes preventius de la suplementació amb Lactobacillus ATCC 55730 fins als 2 i 7 anys. En aquesta tesi, s'utilitzaren mostres de bebès arreplegades longitudinalment, obtinguts a 1 i 12 mesos, 3, 6, 12, 24 mesos i 7 anys, respectivament. A més, s'analitzaren les mostres de llet materna, arreplegades a un mes postpart de les corresponents mares. Mètodes: S'han utilitzat tecnologies de seqüenciació de nova generació dirigides al ARNr 16S, en combinació amb la classificació de les cèl·lules activades, per abordar les respostes de la mucosa cap als bacteris intestinals i de la llet materna. A més, s'utilitzà la seqüenciació d'Illumina MiSeq del gen 16S per descriure la colonització microbiana oral, i es van obtenir mostres longitudinals de saliva de menuts que varen desenvolupar al·lèrgies i d'alguns que es van mantenir saludables. Els nivells de càrrega bacteriana en diferents nínxols microbians s'han obtingut mitjançant la metodologia de qPCR i els nivells totals d'IgA de les mostres fecals es determinaren mitjançant l'immunoassaig ELISA. Resultats i conclusions: La colonització de la cavitat bucal durant la primera infància és transitòria, augmenta la seva complexitat amb el temps, i diversos factors externs influeixen en gran mesura el procés de maduració de la microbiota oral, amb un impacte a curt i llarg termini. Els canvis primerencs en la composició microbiana oral pareixen influir en la maduració del sistema immunològic i el desenvolupament d'al·lèrgies a la infància, així com la presència d'espècies bacterianes específiques pot ser important per a aquest progrés. A més, les respostes d'IgA alterades cap a la microbiota intestinal durant la infància precedeixen a les manifestacions relatives a la malaltia asmàtica i al·lèrgiques durant els primers 7 anys de vida. Per altra banda, el consum de llet materna amb una microbiota de riquesa reduïda al primer mes de vida podria augmentar el risc de desenvolupar al·lèrgia durant la infància. Els resultats observats en aquest estudi haurien de confirmar-se en cohorts humanes més grans i la importància dels factors ambientals post natals que influeixen en el desenvolupament de la microbiota primerenca han de ser més estudiats. Les investigacions futures deuen anar més enllà de la caracterització de la composició de la comunitat bacteriana i investigar els mecanismes funcionals entre els microorganismes colonitzadors primerencs, la maduració del sistema immunològic i el desenvolupament de l'al·lèrgia i l'asma durant la in / [EN] Background: It has been proposed that altered microbial colonization patterns during infancy may be partly responsible for the increase of allergic diseases in developed countries. The gut microbiota differs in composition and diversity during the first months of life in children who later do or do not develop allergic disease. However, little is known about the significance of early mucosal immune responses to the gut microbiota in childhood allergy development, and the findings regarding the protective effect of breastmilk microbiota in the risk of allergy development have been inconclusive. Furthermore, even though the oral cavity is the first site of encounter between a majority of foreign antigens and the immune system, the influence of oral bacteria on allergy development during childhood has not yet been reported. Objectives: The general aim of this thesis was to assess the microbial composition and diversity of oral, fecal and breastmilk samples, together with its interaction with IgA, in order to study the role of microbial development during early childhood in health and allergic disease. Subjects: The infants and mothers included in this study were part of a larger randomized double-blind trial in Sweden, between 2001 and 2003, where potential allergy preventive effects of Lactobacillus reuteri ATCC 55730 were evaluated until 2 and 7 years of age. In this thesis, we used longitudinally collected stool and oral samples from infants, obtained at 1 and 12 months and 3, 6, 12, 24 months and 7 years of age, respectively. Furthermore, we analyzed breastmilk samples, collected at one month post partum, from the corresponding mothers. Methods: Next-generation sequencing technologies targeting the 16S rRNA gene, in combination with cell activated cell sorting, were used in order to address mucosal IgA responses towards gut and breastmilk bacteria. Furthermore, sequencing of the 16S rRNA gene was used in order to describe oral microbiota colonization, in longitudinally obtained saliva samples, from children developing allergy or staying healthy. Bacterial load levels in different microbial habitats were obtained by qPCR methodology and total IgA levels of stool samples were determined by ELISA immunoassays. Results and conclusion: Colonization of the oral cavity during early childhood is transitional, increasing in complexity with time, and several external factors appear to greatly influence oral microbiota maturation, having either a short or a long-term impact. Early changes in oral microbial composition seem to influence immune maturation and allergy development in childhood, and the presence of specific bacterial species may be important for this progress. Furthermore, altered IgA responses towards the gut microbiota during infancy preceded asthma and allergy manifestations during the first 7 years of life, and consumption of breastmilk with a reduced microbial richness in the first month of life may increase the risk for allergy development during childhood. Findings observed here need to be confirmed in larger cohorts and the importance of postnatal environmental factors for early microbiota development should be addressed further. Future research should go beyond characterization of bacterial community composition and investigate the functional mechanisms between early colonizing microorganisms, immune maturation and allergy and asthma development during childhood. / Dzidic, M. (2019). Microbiota development and mucosal IgA responses during childhood in health and allergic disease [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/125479 / TESIS

Au Canada, en 2018-2019 la naissance par césarienne s’élève à 29% dépassant ainsi les taux de 10-15% recommandés par l’Organisation Mondiale de la Santé (OMS). Au cours des dernières années, les naissances par césariennes ne cessent d’accroitre, malgré les risques associés qui sont rapportés dans la littérature pour la mère et l’enfant. Bien que couramment pratiquée, la césarienne reste une intervention chirurgicale avec des risques à court et à long terme. Chez les enfants nés par césarienne, nous retrouvons des taux plus élevés d’asthme, d’obésité, de problème d’allergies etc. Tandis que chez les mères subissant la césarienne, nous retrouvons plus de cas d’infection, d’hémorragie et des risques plus élevées de rupture du placenta et de fausse couche lors des grossesses subséquentes. Puisque la prévalence de la césarienne ne cesse d’augmenter, il est primordial de comprendre son influence sur le développement neurologique de l’enfant. L’association entre le mode d’accouchement et les troubles neurodéveloppementaux tel que le trouble du déficit d'attention et d’hyperactivité (TDAH) chez les enfants est peu étudié avec des résultats souvent contradictoires ou non concluants. Le TDAH est l'un des troubles neurodéveloppementaux les plus courants chez les enfants avec une prévalence mondiale qui s’élève à 7.2% (IC 95% 6.7-7.8). Ainsi, le projet de ce mémoire a pour objectif d’évaluer l’association entre les modes d’accouchements et le TDAH chez l’enfant, tout en palliant les limites méthodologiques observées dans la littérature présentement. Afin de répondre à cette question, le projet évalue trois modes d’accouchements et ainsi recherche l’association entre l’accouchement par césarienne élective, par césarienne d’urgence ou par voie vaginale assistée et le risque de TDAH chez les enfants comparativement à l’accouchement par voie vaginale non assisté. Cette étude de cohorte rétrospective populationnelle tire l’entièreté de ses données de la Cohorte des Grossesses du Québec (CGQ) sur une période allant de 1998 à 2015. L’étude retrouve une augmentation de 6 % (IC 95% 1.03 - 1.10) du risque de TDAH chez les enfants nés par césarienne d’urgence et une augmentation de 12 % (IC 95% 1.06 - 1.19) du risque de TDAH chez les enfants nés par voie vaginale assisté avec ventouse ou forceps. Aucune association (HRa=0.96 IC 95% 0.91 - 1.01) n’a été retrouvée entre l’accouchement par césarienne élective et l’augmentation du risque de TDAH chez les enfants. Par la suite, plusieurs analyses de sensibilités ont été effectuées pour évaluer la robustesse des résultats, mais aucune n’a significativement alterné nos résultats. En conclusion, l’étude démontre l’existence d’une association entre l’accouchement par césarienne d’urgence et par voie vaginale assisté et le risque de TDAH chez les enfants. Ces résultats renforcent la nécessité d’étudier davantage les répercussions de la césarienne et des accouchements assistés avec des instruments sur l’enfant dans un contexte Canadien, où nous retrouvons peu de recherche sur le sujet. / In Canada the rate of caesarean deliveries is about 29 % of all birth in 2018-2019. Despite the known risk of this major surgery, caesarean section rates are continually rising beyond the recommended rate by the world health organization (WHO) of 10 to 15 %. Scientific research has identified several short- and long-term potential risks associated with this procedure for both the child and mother. For the child, researchers have found higher rates of asthma, obesity, and allergy problems. As for the mother, some of these risks include post-partum infection, hemorrhage and higher risks of placental rupture and miscarriages in future pregnancies. Caesarean section rates have been steadily increasing and it is essential to understand its influence on the neurological development of the child. Few studies have investigated the association between the mode of delivery and neurodevelopmental disorders such as attention deficit hyperactivity disorder (ADHD) in children. Currently the literature shows often contradictory or inconclusive results. ADHD is one of the most common neurodevelopmental disorders in childhood with a worldwide prevalence of up to 7.2% (CI 95% 6.7-7.8). Therefore, the project of this thesis aims to evaluate the association between modes of delivery and ADHD in children while overcoming the methodological limitations observed in the literature. To answer this question, the project is evaluating three modes of delivery by looking into the association between elective caesarean, emergency caesarean or assisted vaginal delivery and the risk of ADHD in children compared to unassisted vaginal birth. This prospective population-based study uses data on pregnancies and children from 1998 to 2015 from The Quebec Pregnancy Cohort (QPC). Our adjusted analyses showed that children born via emergency caesarean and assisted vaginal delivery were 6% (CI 95% 1.03 - 1.10) and 12% (CI 95% 1.06 - 1.19) respectively more likely to have ADHD compared to children born via unassisted vaginal delivery. 6 However, no association was found between elective caesarean birth (HRa=0.96 CI 95% 0.91 - 1.01) and the risk of ADHD in children. Several sensitivities analyze were performed to assess the robustness of these findings, but none significantly altered our results. While a caesarean delivery and assisted vaginal birth are essential and lifesaving procedures, they can put children at risk. As showed in this study, emergency caesarean and assisted vaginal birth increase the risk of ADHD in children. These findings reinforce the need for further research to evaluate the impact of caesarean and assisted deliveries on the children especially in a Canadian context where studies are still limited.