Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / A argila encontrada na Barra da Tijuca, Rio de Janeiro, é conhecida por ser muito mole e de peso específico bastante baixo. Passados 17 anos após a execução de um aterro em um dos depósitos argilosos da região, foram realizados ensaios de piezocone e SPT para aferir a espessura atual da camada mole. Tendo-se as espessuras originais da camada, determinadas a partir de ensaios de palheta e SPT na época da realização da obra, foi possível,assim, determinar o recalque que realmente ocorreu. O recalque primário foi calculado através dos parâmetros de compressibilidade previamente conhecidos daquele material. Foi aplicada a correção devido à submersão do aterro e, para isso, foi desenvolvida neste trabalho uma nova metodologia de cálculo para este efeito, considerando a submersão do aterro de forma gradual em todo o seu desenvolvimento matemático. Através da diferença entre o recalque total verificado nos ensaios e o recalque primário calculado, determinou-se a magnitude do recalque secundário. Com isso, estimou-se o OCR secundário do local. Este parâmetro surge da teoria que considera que os recalques secundários também ocorrem devido à variação das tensões efetivas, mas, nesse caso, horizontais. As principais conclusões desta pesquisa apontam para um valor de OCR secundário maior do que aquele já conhecido da argila de Sarapuí, que serviu de base para a referida teoria. Além disso, a proposta de cálculo do efeito da submersão se mostrou fácil e rápida de ser aplicada e forneceu resultados próximos daqueles obtidos pelas metodologias usuais. / The clay found in Barra da Tijuca, Rio de Janeiro, is known to be very soft and also to have very low specific weight. After 17 years from the execution of a landfill on a clay deposits in the region, piezocone and SPT tests were performed to verify the current soft layer thickness. The original layer thicknesses have been determined from vane and SPT tests at the time of construction of the landfill. It was then possible to determine the settlement that has actually occurred. The primary settlement was calculated through the compressibility parameters previously known. A correction on the calculation result was applied due to the submersion effect. A new methodology for calculating this effect was developed in this work, considering the submersion of the landfill occurring gradually, throughout the mathematical development. From the difference between the total settlement observed in the tests and the estimated primary settlement, the magnitude of the secondary settlement was determined. Thus, it was possible to estimate the secondary OCR. This parameter arises from the theory that considers the secondary settlements occurring with the variation of the effective stresses, but, in this case, horizontal stresses. The main conclusions of this research indicate that the value of secondary OCR is greater than that already known for the Sarapuí clay, which formed the basis for this theory. In addition, the proposed calculation of the effect of submersion proved to be quick and easy to apply and provided results similar to those obtained by the usual methods.

Dissertação(mestrado) - Universidade Federal do Rio Grande, Programa de Pós–Graduação em Oceanografia Biológica, Instituto de Oceanografia, 2006. / Submitted by Cristiane Gomides (cristiane_gomides@hotmail.com) on 2013-11-19T11:17:25Z No. of bitstreams: 1 Dissertacao Marcos.pdf: 1118373 bytes, checksum: 7a93dd33316a5f05afa5bd7d2f611436 (MD5) / Approved for entry into archive by Angelica Miranda (angelicacdm@gmail.com) on 2013-11-20T21:49:31Z (GMT) No. of bitstreams: 1 Dissertacao Marcos.pdf: 1118373 bytes, checksum: 7a93dd33316a5f05afa5bd7d2f611436 (MD5) / Made available in DSpace on 2013-11-20T21:49:31Z (GMT). No. of bitstreams: 1 Dissertacao Marcos.pdf: 1118373 bytes, checksum: 7a93dd33316a5f05afa5bd7d2f611436 (MD5) Previous issue date: 2006 / Devido à falta de estruturas rígidas para determinação de idade em crustáceos, métodos que se baseiam na determinação de idade através de freqüência de tamanhos são comumente utilizados. Neste trabalho, foram estudados aspectos do crescimento em laboratório e no ambiente, de juvenis e adultos, do siri-azul Callinectes sapidus, com o objetivo de desenvolver uma metodologia adequada à obtenção do siri-mole (softcrab) no estuário da Lagoa dos Patos. Os indivíduos de laboratório foram coletados e acompanhados individualmente. A biometria foi realizada antes e depois das mudas. A temperatura foi mantida constante (25±2°C) e a salinidade foi mantida a 20±5. Os indivíduos do ambiente foram coletados entre 2002 e 2003 em pontos prédeterminados no estuário da Lagoa dos Patos, com rede de portas. A obtenção das curvas de crescimento se baseou nos modelos de Gompertz e Bertalanffy, neste último utilizou-se o método de determinação de idade através do acompanhamento do deslocamento modal. Foram utilizados aspectos biológicos conhecidos da espécie para validar as curvas de crescimento. O tamanho máximo de largura de carapaça utilizado foi mantido fixo em todas as análises (157,78 para fêmeas e 162,71 para machos). A curva de Gompertz foi aplicada aos indivíduos menores cultivados em laboratório e os parâmetros obtidos foram k=0,0238/dia; co=4,15; longevidade de 2530 dias para machos e k=0,00375/dia; co=4,69; longevidade de 1638 dias para fêmeas. A curva de Bertalanffy foi determinada através da análise de progressão modal. Para os maiores indivíduos cultivados em laboratório os parâmetros e a longevidade obtidos foram k=0,0016/dia; to=-0,7; longevidade de 3117 dias para machos e k=0,0018/dia; to=-12,6; longevidade de 2795 dias para fêmeas. Os parâmetros de crescimento e a longevidade estimados para os animais coletados no ambiente foram k=0,04502/dia; to=-4,23; 1267 dias para machos e k=0,04271/dia; to=-3,71; 1260 dias para fêmeas. Os animais de laboratório exibiram crescimento diferente dos animais do ambiente, as estimativas mostram os siris crescendo menos e tendo longevidades maiores. O sistema de cultivo utilizado para a manutenção dos animais em laboratório mostrou-se eficiente. A obtenção do siri-mole poderá ser uma fonte alternativa de renda para os pescadores artesanais. / The absence of hard structures capable of register age on crustacean, requires the use of methods such as modal progression analysis (MPA) to estimate growth. In this paper, growth and mortality of juveniles and adults of the blue crab Callinectes sapidus were studied through field and laboratory surveys, in attempt to develop an adequate method to obtain softcrabs on the Patos Lagoon estuary. Gompertz and von Bertalanffy curves were used to estimate growth parameters of blue crabs collected on the Patos Lagoon estuary. Crabs obtained from field sampling were kept under laboratory conditions (temperature = 25±2°C, and salinity = 20±5), on a water recirculating system and measured before and after molting. Individuals used for growth and mortality analysis were collected between 2002 and 2003 in predetermined sampling sites by using otter-trawl and renfro nets. Measures consisted of carapace length, taken from the last antherolateral spines (CL in millimeters) and wet weight (W in grams). Maximum carapace lengths used as asymptotic length were taken from long term catch series of larger individuals and used to validate the growth curves (157.78 mm for females and 162.71 mm for males). Growth estimates based on Gompertz model for smaller individuals reared in laboratory, were as follows: k=0.0238; co=4.15; longevity= 2530 days for males and k=0.00375; co=4.69; longevity= 1638 days for females. Growth estimates based on von Bertalanffy growth curve were k=0.0016/day; to=-0.7; longevity= 3117 days for males and k=0.0018/day; to=-12.6; longevity= 2795 days for females. Growth parameters and longevity estimates for the field collected animals were k=0.04502/day; to=-4.23; 1267 days for males and k=0.04271/day; to=-3.71; 1260 days for females. No significant correlation was obtained among laboratory and field animals, suggesting a different growth rate. The Patos Lagoon estuary shows good potential for the establishment of a softcrab facility, as an alternative income for the artisanal fisheries.

This thesis explores the opportunities and constraints to community based planning for neighbourhood conservation in the context of an expanding urban core, using the Mole Hill planning process and draft Concept Plan as a case study. In response to previous public processes, and increasing community activism against top-down revitalization, City of Vancouver Planning Staff were directed in April 1996, to undertake a participatory planning process in order to develop conservation strategies for Mole Hill which balance physical, social, environmental and economic goals. Drawing from a broad range of literature on urban conservation and post-industrial urban change, this thesis begins by providing a rationale for area-based urban conservation within the context of post-war North American and European cities. Further, it outlines some of the conflicts arising from conservation, primarily those conflicts relating to post-war economic restructuring and corresponding socio-economic changes in inner cities specifically, gentrification. The thesis uses cases from inner city communities in Vancouver and New York to examine contemporary trends in neighbourhood conservation, and to explore those elements which contribute to a locally focused and sustainable conservation and revitalization strategy. Three characteristics of successful neighbourhood conservation schemes are abstracted by way of a literature review and are subsequently used as a framework to analyse the Mole Hill case study. These characteristics are: a focus on local involvement; multi-dimensional programs and policies; and an ongoing collaborative approach. The case study reveals the complex and contentious nature of planning for conservation within an expanding urban context. The multiplicity of players, the exigency of issues, the amount and availability of resources, and the often adversarial positions demonstrate the fragmented and complex quality of planning in this context. The study found that in Mole Hill a reliance on top-down methods for conflict negotiation and decision making undermined the effectiveness of the community based model and ultimately led to a fractured set of policies and principles for the neighbourhood. Tensions which were created by a misapplication of power resulted in a polarization between a "Staff' position and a "Working Group" position, thus diluting the idea of a consensus based, collaborative process. Despite these setbacks, a multi-agency and multi-interest approach to the process achieved a number of positive principles and strategies which attempt to address the site as a comprehensive whole, bringing the goals of heritage conservation closer to those of housing and community needs. Finally, the case showed that by drawing on existing community knowledge and expertise the process was able to generate innovative ideas such as those which involve local reinvestment and community economic development initiatives which are directed at social and physical improvement. These ideas challenge traditional notions of conservation and revitalization — which often rely on outside capital investment and /or economic incentives to support conservation — and provide a foundation for more culturally appropriate and sustainable strategies for community based neighbourhood conservation. / Applied Science, Faculty of / Community and Regional Planning (SCARP), School of / Graduate

Despite an IUCN conservation status of critically endangered, Juliana’s golden mole (Neamblysomus julianae) has received no ecological research attention to date. The species urgently requires conservation attention, but a poor understanding its biology, ecology and distribution makes effective conservation planning almost impossible. In light of this, a thorough understanding of the habitat requirements for this habitat specific species was needed. Additionally, the cryptic lifestyle of Juliana’s golden mole has resulted in very few distribution records for the species. Little was known about the animals’ daily and seasonal activity patterns or movement patterns. This study revealed that Juliana’s golden mole is range restricted on account of sandy soils that have a uniform particle size distribution. This feature limits substrate compaction, making tunnelling far easier for these small mammals. In comparison to uninhabited areas, occupied habitat had greater vegetation cover provided by trees and shrubs, and this would be expected to provide a cooler and moister microenvironment. The ecological parameters associated with the species presence were then used, in addition to existing GIS data, to predict regions of potentially suitable habitat. This process revealed large potentially inhabitable areas in the northern parts of South Africa. Preliminary ground-truthing has shown the model to be relatively accurate with three potentially new populations having been identified. Contrary to what has been reported in the literature, Juliana’s golden mole does not appear to be strictly nocturnal. Instead, an adaptive pattern of activity was observed, governed by soil temperature. Body temperature was found to fluctuate to some degree with that of the soil temperature, but fluctuations were regulated by behavioural thermoregulation. Seasonal activity is highly correlated with rainfall. Rain moistens the soil making it significantly softer and far easier to tunnel through. Using this and other information acquired through the course of the study, the thesis culminates with an evaluation of conservation concerns and proposed conservation management actions. Copyright / Dissertation (MSc)--University of Pretoria, 2009. / Zoology and Entomology / unrestricted

After successfully avoiding of the destructive scenario with the demolition of reservoirs and replacing them with parking house, the second question arises. How to stimulate the people's interest in these underground jewels, the interest that would confirm the decision to save reservoirs. One of the possible ways, in which I see a purpose and the one that I decided to chose is to make the reservoirs accessible alongside the pathway and let the natural curiosity of human to do the rest.

Background: The naked mole-rat (NMR) is a long-lived and cancer resistant species. Identification of potential anti-cancer and age related mechanisms is of great interest and makes this species eminent to investigate anti-cancer strategies and understand aging mechanisms. Since it is known that the NMR expresses higher liver mRNA-levels of alpha 2-macroglobulin than mice, nothing is known about its structure, functionality or expression level in the NMR compared to the human A2M. Results: Here we show a comprehensive analysis of NMR- and human plasma-A2M, showing a different prediction in glycosylation of NMR-A2M, which results in a higher molecular weight compared to human A2M. Additionally, we found a higher concentration of A2M (8.3±0.44 mg/mL vs. and 4.4±0.20 mg/mL) and a lower total plasma protein content (38.7±1.79 mg/mL vs. 61.7±3.20 mg/mL) in NMR compared to human. NMR-A2M can be transformed by methylamine and trypsin resulting in a conformational change similar to human A2M. NMRA2M is detectable by a polyclonal antibody against human A2M. Determination of tryptic and anti-tryptic activity of NMR and human plasma revealed a higher anti-tryptic activity of the NMR plasma. On the other hand, less proteolytic activity was found in NMR plasma compared to human plasma.

Faute de connaissances épistémologiques et socio-historiques au sujet du concept de mole et d’une transposition didactique pertinente de ce concept dans les manuels scolaires, les enseignants ne disposent pas des moyens nécessaires pour que leur enseignement soit aussi efficace qu’ils le souhaiteraient. Plusieurs recherches confirment que cet enseignement met habituellement l’accent sur l’aspect quantitatif de ce concept, néglige ses aspects qualitatifs et ne tient pas toujours compte des trois domaines du savoir en chimie : sous-microscopique, macroscopique et symbolique. L’évolution socio-historique de la mole est complexe. En 1900, Ostwald définit la mole comme étant une « masse normale ». En 1961, on reconnait la mole comme étant un concept pour compter les entités sous-microscopiques. En 1971, la mole devient l’unité de la « quantité de matière », sans toutefois que cela soit clairement mentionné dans la définition. Les diverses définitions qui se sont succédées laissent donc l’enseignant perplexe devant le choix d’une définition adéquate. De plus, les confusions linguistiques liées à l’apprentissage de la mole sont nombreuses, telles que des confusions phonétiques (mol et molécule), et des confusions symboliques (n, m, M, N. etc.). Un enseignement de la mole qui ne tient pas compte de ses aspects qualitatif et quantitatif, de l’évolution socio-historique du concept et d’un évitement des erreurs sémantiques risque de provoquer des obstacles didactiques chez les élèves. Par conséquent, l’objectif général de cette recherche de développement était d’étudier l’impact d’une formation portant sur le concept de mole donnée à des enseignants du secondaire sur l’évolution de leurs connaissances et compétences professionnelles et sur la réduction des obstacles didactiques chez les élèves. La formation a été planifiée selon un cycle de DBR (Design Based Research) qui comportait de nombreuses interactions entre l’étudiante-chercheure et les six enseignantes participantes d’une école du Liban. Ce cycle de DBR se subdivisait en cinq méso cycles dans chacun desquels l’étudiante-chercheure animait des activités de perfectionnement. Le premier mésocycleconsistait essentiellement en un microcycle d’analyse et d’exploration ; les 2e, 3e et 4e cycles comportent des microcycles de design et de conception de matériel didactique ainsi que des microcycles d’analyse, d’exploration, d’évaluation et de réflexion ; le dernier mésocycleconsistait surtout en un microcycle d’évaluation et d’exploration. Durant le premier méso cycle, l’analyse des données recueillies lors d’un groupe de discussion, d’une analyse de fiches de préparation et d’une évaluation diagnostique des apprentissages a confirmé la problématique décrite ci-dessus. Durant les trois méso cycles suivants (2e, 3e et 4e), un matériel didactique a été élaboré et mis à l’essai en classe. Chacun de ces méso cycles commençait par un retour réflexif des enseignantes ayant expérimenté le matériel didactique en classe. Par la suite, une activité de perfectionnement de synthèse était animée par l’étudiante-chercheure, une amélioration du matériel d’équipe était effectuée en équipe et une mise à l’essai par des enseignantes était réalisée en classe. Chaque mésocyclese terminait par une évaluation des résultats des élèves. Durant le dernier méso cycle, l’étudiante-chercheure a animé un groupe de discussion et des entretiens d’explicitation durant lequel les enseignantes participantes ont partagé leurs impressions au sujet des effets de la formation sur leur enseignement et sur les apprentissages des élèves. L’analyse et l’interprétation des résultats obtenus semblent montrer des effets positifs sur l’évolution des connaissances et des compétences professionnelles des enseignantes et sur la réduction des obstacles didactiques chez les élèves. Cette recherche présentait néanmoins d’assez nombreuses limites, notamment en raison du petit nombre d’enseignantes participantes. / In the absence of epistemological and socio-historical knowledge about the concept of mole and of a relevant didactic transposition of this concept in textbooks, teachers do not have the means to make their teaching as effective as they would like. Several studies confirm that this teaching usually emphasizes the quantitative aspect of this concept, neglects its qualitative aspects and does not always take into account the three areas of knowledge in chemistry: sub-microscopic, macroscopic and symbolic. The socio-historical evolution of the mole is complex. In 1900, Ostwald defined the mole as a "normal mass". In 1961, the mole was recognized as a concept for counting sub-microscopic entities. In 1971, the mole became the unit of the "quantity of substance", however without this being clearly mentioned in the definition. These various definitions leave the teacher puzzled and looking for an adequate definition. In addition, there are many linguistic confusions related to the learning of the mole, such as phonetic confusions (mol and molecule), and symbolic confusions (n, m, M, N, etc.). A teaching of the mole that does not take into account its qualitative and quantitative aspects, the socio-historical evolution of the concept and an avoidance of semantic errors may cause didactic obstacles for secondary school students. Therefore, the general objective of this development research was to study the impact of a training on the concept of mole given to secondary school teachers on the evolution of their professional knowledge and skills and on the reduction of didactic barriers in secondary school students. The training was planned according to a DBR (Design Based Research) cycle which involved numerous interactions between the student-researcher and the six participating teachers from a Lebanese school. This DBR cycle was subdivided into five meso-cycles in each of which the student-researcher conducted developmental activities. The first meso-cycle consisted essentially of a microcycle of analysis and exploration ; the 2nd, 3rd and 4th cycles include microcycles of design of didactic materials as well as microcycles of analysis and exploration, evaluation and reflection ; the last meso-cycle consisted mainly of a microcycle of evaluation and exploration. During the first meso-cycle, an analysis of the data collected during a focus group, an analysis of the preparation documents and a diagnostic assessment of learning confirmed the problem described above. During the following three meso-cycles (2nd, 3rd and 4th), a didactic material was developed and tested in class. Each of these meso-cycles began with a reflexive feedback from the teachers who had experienced the didactic material in class. Subsequently, a synthesis development activity was facilitated by the student-researcher, an improvement of the team material was carried out by the teachers and a test was conducted in class. Each meso-cycle ended with an assessment of the secondary school students’ achievement. During the last meso-cycle, the student-researcher facilitated a focus group and explanatory interviews during which the participating teachers shared their impressions about the effects of the training on their teaching and on student learning. The analysis and interpretation of the results obtained seem to show positive effects on the evolution of teachers' professional knowledge and skills and on the reduction of didactic obstacles for secondary school students. Nevertheless, this research had quite a few limitations, mainly because of the small number of participating teachers.

After successfully avoiding of the destructive scenario with the demolition of reservoirs and replacing them with parking house, the second question arises. How to stimulate the people's interest in these underground jewels, the interest that would confirm the decision to save reservoirs. One of the possible ways, in which I see a purpose and the one that I decided to chose is to make the reservoirs accessible alongside the pathway and let the natural curiosity of human to do the rest.

[pt] A constante expansão ocupacional de terrenos resulta na eventual construção sobre camadas de argila mole, o que requer a utilização de fundações em estacas e, com isso, maior conhecimento sobre o material do subsolo e a interação entre as estruturas. Em um projeto de fundações, a consideração das cargas transversais atuantes na superfície nem sempre é suficiente, é necessário adicionar as solicitações também impostas ao longo do seu comprimento. Este trabalho relata o caso de um edifício em Ubatuba/SP, que entrou em colapso devido a solicitações laterais no fuste das estacas próximas ao fundo do terreno, local com maior espessura de argila mole. Este movimento foi analisado por meio de quatro métodos convencionais (Tschebotarioff, De Beer e Wallays, Stewart e Goh), além do método numérico utilizando o software Plaxis em modelagens 2D e 3D. Os métodos convencionais apresentaram momentos fletores bastante divergentes entre si, porém todos apresentaram resultados cuja ordem de grandeza foi muito maior que os obtidos por meio da análise numérica. A modelagem bidimensional mostrou que o espaçamento entre estacas no eixo fora do plano é um fator com bastante influência e interfere nos valores resultantes do modelo 2D, outra constatação foi a redução de até 50 por cento dos momentos fletores atuantes quando considerado o efeito de grupo. Os resultados da modelagem 3D mostraram-se concordantes com os da modelagem 2D, chegando a apresentar uma divergência de 2 por cento entre os resultados. Em termos gerais, foi confirmada a maior solicitação nas estacas mais próximas ao limite posterior do terreno, onde se encontra a camada mais espessa de argila, o que gerou momentos maiores do que aqueles gerados pela carga transversal da superestrutura. / [en] The constant expansion of land occupation leads to the eventual construction over soft clay layers, requiring the use of foundation piles and thus, greater understanding of the subsurface material and the interaction between the structures, especially in regards to the induced loads and displacements. Naturally, the loads from the overhead structure are transmitted to the foundation, causing it to suffer from the action of axial and transverse loads. In a foundation design, the consideration of transverse loads acting on the surface is not always sufficient, making it necessary to include the loads imposed along its length as well. This study depicts the case of a building in Ubatuba/SP that collapsed due to lateral stresses along the middle section of the piles, located close to the farthest site boundary where a thicker layer of soft clay can be found. This movement was analysed using four conventional methods (Tschebotarioff, De Beer and Wallays, Stewart and Goh), as well as a numerical method for 2D and 3D modelling on the Plaxis software. The conventional methods presented diverging results relative to each other, of which. However, all four methods produced results with orders of magnitude much greater than those obtained through numerical analysis. The bidimensional model showed that the spacing between piles in the out-of-plane axis is a factor of great influence and interferes in the simplifications of the 2D model. Another finding was a reduction of up to 50 percent of the acting bending moments when considering the group effect. The results from the 3D model were in agreement with those from the 2D model, exhibiting only a 2 percent difference. In general, a greater load on the piles closest to the farthest limit of the site was confirmed, located on the thickest clay layer that generated bending moments greater than those generated from the transverse load of the structure.

Many imprinted genes are necessary for normal human development. Approximately 70 imprinted genes have been identified in humans. I developed a novel approach to identify candidate imprinted genes in humans using the premise that imprinted genes are often associated with nearby parent-of-origin-specific DNA differentially methylated regions (DMRs). I identified parent-of-origin-specific DMRs using sodium bisulfite-based DNA (CpG) methylation profiling of uniparental tissues, mature cystic ovarian teratoma (MCT) and androgenetic complete hydatidiform mole (AnCHM), and biparental tissues, blood and placenta. In support of this approach, the CpG methylation profiling led to the identification of parent-of-origin-specific differentially methylated CpG sites (DMCpGs) in known parent-of-origin-specific DMRs. I found new DMRs for known imprinted genes NAP1L5 and ZNF597. Most importantly, I discovered many new DMCpGs, which were associated with nearby genes, i.e., candidate imprinted genes. Allelic expression analyses of one candidate imprinted gene, AXL, suggested polymorphic imprinting of AXL in human blood.