京都大学 / 0048 / 新制・課程博士 / 博士(理学) / 甲第22277号 / 理博第4591号 / 新制||理||1659(附属図書館) / 京都大学大学院理学研究科化学専攻 / (主査)教授 北川 宏, 教授 竹腰 清乃理, 教授 吉村 一良 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DFAM

The title of this master´s thesis is the elaboration of a design for a newly builded building of civil importance - a cultural center. Object is 3 storey, situated on the flat plot in the wider center of Trencin. The center includes a concert hall and facilities for training and recording musical productions. The concert hall forms the left part of the building and consists of a stage, adjacent auditoriums with 352 seats, communication spaces and backgrounds for needs of performance. On the right side there are facilities for testing and recording of music with its own facilities, an entrance hall with a café and a seating area. The construction system consists of a monolithic vertical and horizontal RC structure, complemented in a concert hall with a beam ceiling. The roof is flat in 2 levels, the facade and the envelope of the building is a combination of glass facade and the ETICS insulation system, the highest part of the building is created by cetris tiles. Drawing part is drawn in AutoCAD, other programs used (eg to calculate physical properties of structures etc.) are DEKsoft, WDLS.

The objective of this master´s thesis is a working out of project documentation of building "New build of chalet" containing all the requirements according to currently valid orders and standards. The project deals with a construction of a four storey chalet with 17 rooms for guests and restaurant. The object is situated in Dolní Lomná on a sloping terrain. Structural system is monolith skelet with gabled roof. The project documentation includes drawing, texting and calculation part.

A systematic NMR cryo-porometry and -diffusometry study using nitrobenzene as a probe liquid is carried out in order to characterise pore structures of hierarchically-organised porous silica monolith possessing mesopores along with a 3D bicontinuous macropore network. The result obtained from NMR cryoporometry shows the presence of a relatively wide mesopore size distribution of 10-35 nm. Furthermore, NMR cryodiffusometry indicates that whilst the mesopores are highly tortuous (Tmeso ≈6), they have little influence on the overall tortuosity of the material (Tmacro ≈1.5), which is largely dominated by the macropores (Toverall ≈1.7).

京都大学 / 0048 / 新制・課程博士 / 博士(理学) / 甲第19519号 / 理博第4179号 / 新制||理||1600(附属図書館) / 32555 / 京都大学大学院理学研究科化学専攻 / (主査)准教授 中西 和樹, 教授 北川 宏, 教授 有賀 哲也 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM

The ongoing energy and environmental crises have pushed the transportation sector, a major greenhouse gas emitter, to seek sustainable fuel and technology alternatives. Natural gas and bio-methane are potential alternatives with numerous advantages over conventional fuels. Adsorbed natural gas (ANG) technology uses porous adsorbent material to store methane efficiently at lower pressures. An issue limiting this technology is the lack of compact tanks with efficient adsorbent packing that increase storage capacity. This study addresses the need for more compact ANG tanks by creating novel binder-less monolithic activated carbon monolith adsorbents with targeted porosity. A template is produced using 3D printing and a commercially available phenolic resin as a filling material. Upon thermal treatment, the 3D-printed template combusts with molecular oxygen in its structure, and the resin is transformed into activated carbon by pyrolysis. Longer activation times led to higher BET surface areas. However, after activation periods beyond 15 minutes, the surface area increase is obtained at the expense of a higher burn-off, which affects the material density. Adsorption of 0.04g/g of methane was measured at 30 bar and 298 K on the activated carbon monolith with the highest BET surface area (516 m2/g). Results in the same conditions on a super high surface area Maxsorb activated carbon were 0.13g/g. Although the methane capacity obtained is lower than in a commercial sample, it was demonstrated that producing an activated carbon monolith with tailored porosity is possible. New techniques for activation should be studied to enhance their gravimetric capacity to make ANG competitive.

The analysis of proteins in biological fluids by capillary electrophoresis (CE) is of interest in clinical chemistry. However, due to low analyte concentrations and poor concentration limits of detection (CLOD), protein analysis by this technique is frequently challenging. Coupling preconcentration techniques with CE greatly improves the CLOD. An on-line preconcentration-CE method that can selectively preconcentrate any protein for which an antibody is available would be very useful for the analysis of low abundance proteins and would establish CE as a major tool in biomarker discovery. To accomplish this, an on-line protein G monolithic preconcentrator CE system for enrichment and separation of proteins was developed. This system proved effective for on-line sample extraction, clean-up, preconcentration, and CE of IgG in human serum. IgG from diluted (500 and 65,000 times) human serum samples was successfully analyzed using this system. The approach can be applied to the on-line preconcentration and analysis of any protein for which an antibody is available. The desire to separate all proteins present in human tissues, cells and biological fluids has challenged the separation research community for many years. The difficulty of this task resides in the complexity of the sample. Blood serum, for instance, may express up to 10,000 proteins with an estimated dynamic range of 9 orders of magnitude. Additionally, most of these proteins are present at very low concentrations (ng/mL). Identification and quantification of low abundance proteins is hindered by the presence of high abundance proteins, such as human serum albumin (HSA) and immunoglobulins (IgG). Therefore, in most cases, removal of the high abundance proteins or enrichment of low abundance proteins is necessary prior to the analysis of low abundance proteins. To address this, a coupled affinity-hydropobic monolithic column for the simultaneous removal of IgG, preconcentration of low abundance proteins, and separation by capillary zone electrophoresis was designed. The system proved to be very reproducible. The run-to-run %RSD values for migration time and peak area were less than 5%, which is typical of CE. Finally, a new method was developed to prepare monoliths with anion exchange functionality. Polymer monoliths were prepared by in situ polymerization of methacrylate monomers. The monoliths were coated with a water soluble polymer and used for the analysis of proteins. Using this approach, a model monolith was prepared. Subsequent coating yielded a monolith with quaternary ammonium groups on the surface, which was confirmed by strong anodic electroosmotic flow. Analysis of standard proteins by ion exchange LC and CEC was demonstrated. This simple and rapid method for surface modification opened new avenues for the preparation of monoliths with a broad range of functionalities.

This dissertation focuses on improving the chromatographic efficiency of polymeric organic monoliths by characterizing and optimizing the bed morphology. In-situ characterization techniques such as capillary flow porometry (CFP), 3-dimensional scanning electron microscopy (3D SEM) and conductivity measurements were developed and implemented to quantitatively characterize the morphology of poly(ethylene glycol) diacrylate (PEGDA) monoliths. The CFP measurements for monoliths prepared by the same procedure in capillaries with different diameters (i.e., 75, 150, and 250 μm) clearly showed a change in average through-pore size with capillary diameter, thus, certifying the need for in-situ measurement techniques. Serial sectioning and imaging of PEGDA monoliths using 3D SEM gave quantitative information about the average pore size, porosity, radial heterogeneity and tortuosity of the monolith. Chromatographic efficiency was better for a monolith with smaller average pore size (i.e., 5.23 μm), porosity (i.e., 0.49), radial heterogeneity (i.e., 0.20) and tortuosity (i.e., 1.50) compared to another monolith with values of 5.90 μm, 0.59, 0.50 and 2.34, respectively. Other than providing information about monolith morphology, these techniques also aided in identifying factors governing morphological changes, such as capillary diameter, polymerization method, physical/chemical properties of the pre-polymer constituents and weight proportion of the same. A statistical model was developed for optimizing the weight proportion of pre-polymer constituents from their physical/chemical properties for improved chromatographic efficiency. Fabricated PEGDA columns were used for liquid chromatography of small molecules such as phenols, hydroxyl benzoic acids, and alkyl parabens. The chromatographic retention mechanism was determined to be principally reversed-phase (RP) with additional hydrogen bonding between the polar groups of the analytes and the ethylene oxide groups embedded in the monolith structure. The chromatographic efficiency measured for a non-retained compound (uracil) was 186,000 plates/m when corrected for injector dead volume. High resolution gradient separations of selected pharmaceutical compounds and phenylurea herbicides were achieved in less than 18 min. Column preparation was highly reproducible, with relative standard deviation (RSD) values less than 2.1%, based on retention times of the phenol standards (3 different columns). A further improvement in chromatographic performance was achieved for monoliths fabricated using a different polymerization method, i.e., living free-radical polymerization (LFRP). The columns gave an unprecedented column performance of 238, 000 plates/m for a non-retained compound under RP conditions.

The project discuss mass and monomateriality through residential case studies of modular masonry.

With great advancements in cloud computing, the microservice architecture has become a promising architectural style for enterprise software. It has been proposed to cope with problems of the traditional monolithic architecture which includes slow release cycles, limited scalability and low developer productivity. Therefore, this thesis aims to investigate the affordances and challenges of adopting microservices as well as the difference in performance compared to the monolithic approach at one of Sweden’s largest banks, SEB - the Scandinavian Individual Bank. The investigation consisted of a literature study of research papers and official documentation of microservices. Moreover, two applications were developed and deployed using two different system architectures - a monolithic architecture and a microservice architecture. Performance tests were executed on both systems to gather quantitative data for analysis. The two metrics investigated in this study were response time and error rate. The results indicate the microservice architecture has a significantly higher error rate but a slower response time than the monolithic approach, further strengthening the results of Ueda et. al. [47] and Villamizar et. al. [48]. The findings have then been discussed with regards to the challenges and complexity involved in implementing distributed systems. From this study, it becomes clear the complexity shifts from inside the application out towards infrastructure with a microservice architecture. Therefore, microservices should not be seen as a silver bullet. Rather, the type of architecture is highly dependent on the scope of the project and the size of the organization. / Med stora framstegen inom molntjänster har microservice arkitekturen kommit att bli en lämplig kandidat för utveckling av företagsprogramvara. Denna typ av systemarkitektur har föreslagits att lösa de problem som den traditionella monolitiska arkitekturen medför; långsamma lanseringar, begränsad skalbarhet och låg produktivitet. Således fokuserar denna avhandling på att utforska de möjligheter samt utmaningar som följer vid adoptering av microservices samt skillnaden i prestanda jämfört med den monolitiska arkitekturen. Detta undersöktes på en av Sveriges största banker, SEB, den Skandinaviska Enskilda Banken. Utredningen bestod av en litteraturstudie av vetenskapliga artiklar samt officiell dokumentation för microservices. Dessutom utvecklades och lanserades två applikationer byggt med två olika typer av systemarkitektur - en som monolitisk arkitektur och den andra som en microservice arkitektur. Prestandatest utfördes sedan på båda systemen för att samla kvantitativ data för analys. De två nyckelvardena som undersöktes i denna studie var responstid och felfrekvens. Resultaten indikerar att microservice arkitekturen har en signifikant högre felfrekvens men en långsammare responstid än den monolitiska arkitekturen, vilket stärker resultaten av Ueda et. al. [47] och Villamizar et. al. [48]. Forskningsresultaten har diskuterats med hänsyn till den komplexitet och de utmaningar som följer vid implementering av distribuerade system. Från denna studie blir det tydligt att komplexiteten i en microservice arkitektur skiftar från inuti applikationen ut till infrastrukturen. Således borde microservices inte ses som en silverkula. Istället är valet av systemarkitektur strikt beroende på omfattningen av projektet samt storleken på organisationen i fråga.