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Regulation of Distal Lung Fluid Absorption by Membrane ComponentsBeard, LaMonta L. 28 November 2011 (has links)
No description available.
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Consequences of Gill Remodeling on Na+ Transport in Goldfish, Carassius auratusBradshaw, Julia 08 February 2011 (has links)
Goldfish undergo an adaptive morphological change in their gills involving the reversible growth and loss of a mass of cells (interlamellar cell mass, ILCM) in between the lamellae depending on oxygen demand, which can be altered by the environment or metabolic demands of the individual. The ILCM contributes to decreased passive Na+ efflux across the gill. Active uptake is maintained by the re-distribution of the ionocytes expressing Na+-uptake relevant genes (NHEs and H+-ATPase) to the outer edge of the ILCM where they can establish contact with the external environment and/or lamellar epithelium. This adaptation is thought to be partly responsible for the extreme anoxia tolerance demonstrated by goldfish, which they experience on a seasonal basis living in a pond environment. Hypoxia and hypercapnia are frequently encountered in such freshwater environments and as such, the effect of the ILCM on the capacity for acid-base regulation was evaluated. Differences in the time course of acid excretion to the environment without effect on systemic pH regulation were likely the result of the ILCM.
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Consequences of Gill Remodeling on Na+ Transport in Goldfish, Carassius auratusBradshaw, Julia 08 February 2011 (has links)
Goldfish undergo an adaptive morphological change in their gills involving the reversible growth and loss of a mass of cells (interlamellar cell mass, ILCM) in between the lamellae depending on oxygen demand, which can be altered by the environment or metabolic demands of the individual. The ILCM contributes to decreased passive Na+ efflux across the gill. Active uptake is maintained by the re-distribution of the ionocytes expressing Na+-uptake relevant genes (NHEs and H+-ATPase) to the outer edge of the ILCM where they can establish contact with the external environment and/or lamellar epithelium. This adaptation is thought to be partly responsible for the extreme anoxia tolerance demonstrated by goldfish, which they experience on a seasonal basis living in a pond environment. Hypoxia and hypercapnia are frequently encountered in such freshwater environments and as such, the effect of the ILCM on the capacity for acid-base regulation was evaluated. Differences in the time course of acid excretion to the environment without effect on systemic pH regulation were likely the result of the ILCM.
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Consequences of Gill Remodeling on Na+ Transport in Goldfish, Carassius auratusBradshaw, Julia 08 February 2011 (has links)
Goldfish undergo an adaptive morphological change in their gills involving the reversible growth and loss of a mass of cells (interlamellar cell mass, ILCM) in between the lamellae depending on oxygen demand, which can be altered by the environment or metabolic demands of the individual. The ILCM contributes to decreased passive Na+ efflux across the gill. Active uptake is maintained by the re-distribution of the ionocytes expressing Na+-uptake relevant genes (NHEs and H+-ATPase) to the outer edge of the ILCM where they can establish contact with the external environment and/or lamellar epithelium. This adaptation is thought to be partly responsible for the extreme anoxia tolerance demonstrated by goldfish, which they experience on a seasonal basis living in a pond environment. Hypoxia and hypercapnia are frequently encountered in such freshwater environments and as such, the effect of the ILCM on the capacity for acid-base regulation was evaluated. Differences in the time course of acid excretion to the environment without effect on systemic pH regulation were likely the result of the ILCM.
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Consequences of Gill Remodeling on Na+ Transport in Goldfish, Carassius auratusBradshaw, Julia January 2011 (has links)
Goldfish undergo an adaptive morphological change in their gills involving the reversible growth and loss of a mass of cells (interlamellar cell mass, ILCM) in between the lamellae depending on oxygen demand, which can be altered by the environment or metabolic demands of the individual. The ILCM contributes to decreased passive Na+ efflux across the gill. Active uptake is maintained by the re-distribution of the ionocytes expressing Na+-uptake relevant genes (NHEs and H+-ATPase) to the outer edge of the ILCM where they can establish contact with the external environment and/or lamellar epithelium. This adaptation is thought to be partly responsible for the extreme anoxia tolerance demonstrated by goldfish, which they experience on a seasonal basis living in a pond environment. Hypoxia and hypercapnia are frequently encountered in such freshwater environments and as such, the effect of the ILCM on the capacity for acid-base regulation was evaluated. Differences in the time course of acid excretion to the environment without effect on systemic pH regulation were likely the result of the ILCM.
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Y It Matters: Sex Differences in Fetal Lung DevelopmentLaube, Mandy, Thome, Ulrich H. 13 June 2023 (has links)
Within this review, sex-specific differences in alveolar epithelial functions are discussed
with special focus on preterm infants and the respiratory disorders associated with premature birth.
First, a short overview about fetal lung development, the challenges the lung faces during perinatal
lung transition to air breathing and respiratory distress in preterm infants is given. Next, clinical
observations concerning sex-specific differences in pulmonary morbidity of human preterm infants
are noted. The second part discusses potential sex-specific causes of pulmonary complications,
including pulmonary steroid receptors and local lung steroid metabolism. With regard to pulmonary
steroid metabolism, it is important to highlight which steroidogenic enzymes are expressed at which
stage during fetal lung development. Thereafter, we review the knowledge concerning sex-specific
aspects of lung growth and maturation. Special focus is given to alveolar epithelial Na+
transport as
a driver of perinatal lung transition and the sex differences that were noted in this process.
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Albumin Stimulates Epithelial Na+ Transport and Barrier Integrity by Activating the PI3K/AKT/SGK1 PathwayLaube, Mandy, H. Thome, Ulrich 05 December 2023 (has links)
Albumin is a major serum protein and is frequently used as a cell culture supplement. It is
crucially involved in the regulation of osmotic pressure and distribution of fluid between different
compartments. Alveolar epithelial Na+
transport drives alveolar fluid clearance (AFC), enabling air
breathing. Whether or not albumin affects AFC and Na+
transport is yet unknown. We therefore
determined the acute and chronic effects of albumin on Na+
transport in fetal distal lung epithelial
(FDLE) cells and the involved kinase pathways. Chronic BSA treatment strongly increased epithelial
Na+
transport and barrier integrity in Ussing chambers. BSA did not elevate mRNA expression of
Na+
transporters in FDLE cells after 24 h. Moreover, acute BSA treatment for 45 min mimicked the
chronic effects. The elevated Na+
transport was caused by an increased maximal ENaC activity, while
Na,K-ATPase activity remained unchanged. Acute and chronic BSA treatment lowered membrane
permeability, confirming the increased barrier integrity observed in Ussing chambers. Western blots
demonstrated an increased phosphorylation of AKT and SGK1, and PI3K inhibition abolished the
stimulating effect of BSA. BSA therefore enhanced epithelial Na+
transport and barrier integrity by
activating the PI3K/AKT/SGK1 pathway
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Study of protein in the respiratory chain by IR spectroscopy and electrochemistryNeehaul, Yashvin 13 September 2012 (has links) (PDF)
The field of molecular bioenergetics deals with the energy transduction in biological cells. In this project, respiration and more specifically proton and sodium pumping enzymes and their coupling to electron transfer have been in focus. First we have been interested in the Na+-pumping NADH:quinone reductase from Vibrio cholerae which is the entry site of electrons in the respiratory chain of several pathogens. The role of specific flavin cofactors and amino acids involved in Na+ transfer has been shown in a combined IR spectroscopic and electrochemical approach. The interaction between proteins, namely the cytochrome c552 and the CuA fragment from the terminal ba3 oxidase from the organism Thermus thermophilus was then investigated. Structural reorganization during electron transfer was revealed by IR spectroscopy. Finally, in the third part of the project the interaction within the bc1-aa3 supercomplex from the respiratory chain from Corynebacterium glutamicum was analyzed.
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Stabilization of Hypoxia Inducible Factor by Cobalt Chloride Can Alter Renal Transepithelial TransportNag, Subhra Sankar 20 September 2018 (has links)
No description available.
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Study of protein in the respiratory chain by IR spectroscopy and electrochemistry / Etude des interactions des protéines dans la chaîne respiratoire par spectroscopie IR et par électrochimieNeehaul, Yashvin 13 September 2012 (has links)
Le domaine de la bioénergie moléculaire concerne le transfert et le stockage d’énergie dans les cellules biologiques. Ce projet s’articule autour de la respiration et plus précisément le mécanisme de pompage de sodium et de protons, et son couplage au transfert d’électrons. Premièrement, nous nous sommes intéressés au pompage d’ions sodium par la NADH : quinone oxidoreductase de la bactérie Vibrio cholerae. L’importance de flavines spécifiques et des résidus acides dans le transfert de sodium ont été démontrée. Par la suite, l’interaction entre protéines, notamment le cytochrome c552 et le fragment CuA de l’oxidase de type ba3 de l’organisme Thermus thermophilus a été étudié. Une réorganisation structurelle induit par le transfert d’électron a été démontrée par la spectroscopie IRTF différentielle. Enfin, dans la dernière partie de ce travail, l’interaction au sein du supercomplex bc1-aa3 de la chaîne respiratoire du Corynebacterium glutamicum a été analysée. / The field of molecular bioenergetics deals with the energy transduction in biological cells. In this project, respiration and more specifically proton and sodium pumping enzymes and their coupling to electron transfer have been in focus. First we have been interested in the Na+-pumping NADH:quinone reductase from Vibrio cholerae which is the entry site of electrons in the respiratory chain of several pathogens. The role of specific flavin cofactors and amino acids involved in Na+ transfer has been shown in a combined IR spectroscopic and electrochemical approach. The interaction between proteins, namely the cytochrome c552 and the CuA fragment from the terminal ba3 oxidase from the organism Thermus thermophilus was then investigated. Structural reorganization during electron transfer was revealed by IR spectroscopy. Finally, in the third part of the project the interaction within the bc1-aa3 supercomplex from the respiratory chain from Corynebacterium glutamicum was analyzed.
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