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Identificação e mapeamento de famílias de DNA repetitivo em Characidium sp. aff. C. vidali (Teleostei, Characiformes) e sua atuação na evolução dos cromossomos BNobile, Maria Lígia Marques de Oliveira January 2019 (has links)
Orientador: Fausto Foresti / Resumo: Characidium é um grupo de peixes amplamente distribuídos pela região Neotropical, embora seja considerado o mais especioso dentro de Crenuchidae, do ponto de vista citogenético o número de espécies investigadas ainda é baixo, o que dificulta a caracterização quanto a organização cromossômica do gênero. Em relação ao número diploide, as espécies de Characidium conservaram um cariótipo com 2n = 50 cromossomos, do tipo metacêntricos e submetacêntricos (com exceções), o que resulta em uma macroestrutura homogênea para o grupo. Porém, investigações utilizando sequências repetitivas têm contribuído para ilustrar que a organização microestrutural cromossômica pode diferir entre as espécies, refletindo o hábito destes peixes constituírem populações pequenas e isoladas em cabeceiras de riachos. Adicionalmente, algumas espécies de Characidium também foram descritas portando cromossomos B em seus cariótipos, e a utilização de ferramentas citomoleculares têm contribuído para explorar quanto a origem e evolução destes componentes cariotípicos. Neste sentido, o objetivo do presente estudo foi agregar técnicas citomoleculares com resultados de sequenciamento massivo, para tentar compreender a ocorrência de cromossomos B no genoma de Characidium sp. aff. C. vidali. Os resultados obtidos mostraram que i) o mapeamento físico de diferentes sondas de DNA repetitivo contribuíram não apenas para caracterizar o cariótipo da espécie em estudo, como também adicionaram mais informações quanto a organi... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Characidium is a group of fish widely distributed in the Neotropical region, although it is considered the most specious within Crenuchidae, from the cytogenetic point of view the number of species investigated is still low, which makes it difficult to characterize the chromosomal organization of the genus. In relation to the diploid number, Characidium species retained a karyotype with 2n = 50 chromosomes, metacentric and submetacentric (with exceptions), resulting in a homogeneous macrostructure for the group. However, investigations using repetitive sequences have contributed to illustrate that the chromosomal microstructural organization may differ between species, reflecting the habit of these fish constituting small and isolated populations in headwaters of streams. In addition, some species of Characidium have also been described carrying B chromosomes in their karyotypes, and the use of cyto-molecular tools has contributed to explore the origin and evolution of these karyotype components. In this sense, the objective of the present study was to aggregate cyto-molecular techniques with massive sequencing results to try to understand the occurrence of B chromosomes in the genome of Characidium sp. aff. C. vidali. The results showed that i) the physical mapping of different repetitive DNA probes contributed not only to characterize the karyotype of the species under study, but also added more information about the organization and evolution of the chromosomal microstruct... (Complete abstract click electronic access below) / Doutor
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A composição da comunidade bacteriana do solo como fator determinante na micorrização de cana-de-açúcar por Glomus clarum / The bacterial community composition of soil as a factor in mycorrhizal sugarcane by Glomus clarumPedro Avelino Maia de Andrade 19 June 2013 (has links)
A cana-de-açúcar é uma das principais culturas do sistema agrícola brasileiro, e apresenta-se atualmente em plena expansão. Porém o uso do solo e a implementação de diferentes tecnologias de manejo têm originado alterações no equilíbrio ambiental, onde importantes interações microbianas ocorrem de forma essencial para o desenvolvimento vegetal. Dentre a vasta diversidade de microrganismos do solo, destacam-se os fungos micorrízicos, organismos intimamente associados as raízes das plantas, auxiliando a mesma, dentre outras formas, na obtenção de água e nutrientes. Estes fungos, no entanto, interagem também com outros organismos do solo, como por exemplo, com a comunidade bacteriana presente neste ambiente. Desta forma, o presente trabalho buscou estudar a dinâmica de interação entre cana-de-açúcar e o fungo micorrízico arbuscular (FMA) G.clarum em solos com diferentes composições da comunidade bacteriana. A metodologia utilizada foi a \'diluição para extinção\', onde diluições seriadas (10-1; 10-3; 10-6 e 10-9) de um solo natural foram usadas para inocular o solo estéril. Sobre esta base, foi monitorada pelo período de 60 dias, a colonização da planta pelo FMA e a estruturação das comunidades bacterianas. Como resultado, foi observada uma maior colonização das raízes de cana-de-açúcar para os tratamentos inoculada com menores diluições da comunidade original (solo natural e diluições 10-1 e 10-3), sendo da mesma forma observada uma distinção entre as comunidades bacterianas destes tratamentos para os demais. Estabelecendo correlações entre os grupos microbianos e as taxas de colonização micorrízica, foi possível nomear, com base no sequenciamento massivo da região V6 do gene ribossomal 16S DNAr, a alteração conjunta da micorrização com mudanças nos grupos de Actinobacteria,Bacteriodetes,Firmicutes,Proteobacteria,Verrucomicrobiae Acidobacteria. Concluindo, este trabalho demonstra a dependência que um processo importante, como a micorrização, possui da comunidade bacteriana do solo, e indica que em áreas degradadas, com menores níveis de diversidade bacteriana, tal processo pode ocorrer com menor eficiência. / Sugarcane is an important Brazilian agricultural system crop and presents currently booming. Nevertheless, land use, and implementation of different management technologies have originated changes in environmental balance, where important microbial interactions occur as essential for plant development. Among the wide diversity of soil microorganisms, the mycorrhizal fungi is highilighted as organisms closely associated with plant roots, helping plants, in any way, to obtain water and nutrients. These fungi however, also interact with other soil organisms, such as for example, bacterial community in these environments. Thus, the present work aimed to study the dynamics of interaction between sugarcane and arbuscularmycorrhizal fungi (AMF) Glomusclarum in soils with different compositions of the bacterial community. The methodology used was \"dilution to extinction\", where serial dilutions (10-1, 10-3, 10-6 and 10-9) of a natural soil were used to inoculate a sterile soil. On this basis, were monitored along a period of 60 days, plant colonization by AMF, and structure of bacterial communities. As a result, we observed a higher colonization of roots of cane sugar for treatments inoculated with lower dilutions of the original community (natural soil and dilutions 10-1 and 10-3), and likewise observed a distinction between these bacterial communities treatments to others. Establishing correlations between microbial groups with observed rates of colonization, it was possible to name, based on the massive sequencing of the region V6 ribosomal gene 16S rDNA, the joint amendment of mycorrhiza with changes in groups of Actinobacteria; Bacteriodetes; Firmicutes, Proteobacteria; Verrucomicrobia and Acidobacteria. In conclusion, this work demonstrates the dependence of an important process, as the AMF, has tosoil bacterial community, and indicates that degraded areas, with lower levels of bacterial diversity, such a process can occur with lower efficiency.
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LASZLO @ GALAXY - Um protótipo de serviço de montagem de genomas a partir de dados de sequenciamento de próxima geração (NGS) / LASZLO @ GALAXY - A genome assembly service prototype using Next-Generation Sequencing (NGS) dataRibeiro, Antonio Cláudio Bello January 2012 (has links)
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Previous issue date: 2012 / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Vice Direção de Ensino, Informação e Comunicação. Rio de Janeiro, RJ, Brasil. / As tecnologias NGS (Next-Generation Sequencing), desenvolvidas para reduzir o custo e o tempo do processo de sequenciamento, geram uma grande massa de dados, a um custo relativamente baixo e com grande acurácia. No entanto, as leituras curtas, por elas produzidas, dificultam sobremaneira o processo de montagem de genomas, originando novos problemas computacionais. Para tentar suplantar esses desafios, várias ferramentas de software estão disponíveis e continuam a ser desenvolvidas. Cada um desses pacotes possui vantagens e desvantagens e, na maioria das vezes, se apresenta como uma solução individual, não estando integrado a outros. Além disso, tipicamente é exigido um conhecimento mais avançado de informática para a sua correta instalação, configuração e operação; o que, nem sempre, é a realidade do usuário final. Neste contexto, o projeto nomeado LASZLO (Linkage of Assembly Scripts Zero-costed and with License Opened) @ GALAXY propõe combinar diferentes ferramentas de tratamento de dados de NGS de uso livre, na forma de um protótipo básico de serviço de montagem de genomas, buscando facilitar o trabalho do usuário através da disponibilização de uma interface Web, sugestões de parametrização e de fluxos de trabalho para esse tipo de análise. Tomando por base o framework Galaxy, foram agregados fluxos de trabalho para montagens de dados de sequenciamento reais de diferentes organismos e provenientes das tecnologias Illumina, SOLiD™ e 454. O caráter aplicado do projeto originou soluções pontuais para atender a necessidades específicas, as quais foram reunidas sob o módulo NGS: LASZLO's Sandbox, uma "caixa de ferramentas" especialmente designada às abordagens de montagem do tipo de novo e com auxílio de genoma de referência. Durante a pesquisa, o protótipo LASZLO @ GALAXY processou, por exemplo, dados de sequenciamento de Leishmania amazonensis, contribuindo para um primeiro processo de avaliação do genoma do referido organismo. Atualmente, observa-se que a produção de dados não é o mais o "gargalo" em projetos de sequenciamento, mas sim o fluxo de análise subsequente sobre o material obtido. Muitas vezes, tais dados não se traduzem imediatamente em expansão do conhecimento biológico, devido às dificuldades encontradas pelo biólogo experimental em lidar, não somente com a miríade de ferramentas disponíveis, mas também com fatores como a inerente necessidade de integração entre elas e a implementação de infra-estrutura adequada para a sua operação. Os resultados obtidos no projeto indicam que o sistema proposto, vislumbrado como um eventual serviço institucional ou mesmo de menor âmbito, pode se tornar um aliado do usuário final quanto à manipulação dos dados de NGS. / The NGS (Next-Generation Sequencing) technologies, designed to reduce sequencing process costs and time, generate a huge amount of data, at a relatively low cost and with great accuracy. However, the produced short reads strongly difficult the genome assembly process, originating new computational issues. To overcome those challenges, there are several software tools available and continuously being developed. Each of these tools presents advantages and disadvantages and most of them are isolated, not integrated solutions. Moreover, typically it is required a higher level of computer-literacy for their proper installation, configuration and usage, which, not always, is the end-user reality. In this context, the project named LASZLO (Linkage of Assembly Scripts Zero-costed and with License Opened) @ GALAXY suggests to combine different open source tools for NGS data handling, as a basic prototype service for genome assembly, aiming at simplifying the end-user task by providing a Web interface, suggestions of parametrization and workflows for this kind of analysis. Based on the Galaxy framework, some workflows for the assembly of real sequencing data from different organisms and produced by the Illumina, SOLiD™ and 454 technologies were aggregated. Also, due to the applied characteristic of the project, a few punctual solutions were generated to address specific needs. Those solutions were encapsulated in the NGS: LASZLO's Sandbox module, a "toolbox" especially tailored for the de novo and reference-guided assembly approaches. During the research, the LASZLO @ GALAXY prototype processed, for instance, sequencing data of the Leishmania amazonensis organism, contributing for a first evaluating process of its genome. Presently, it's noticed that the data generation is no longer the "bottleneck" of the sequencing projects, but the downstream data analysis. Frequently, the acquired data is not immediately translated into biological knowledge expansion, due to the obstacles met by the experimental biologist when dealing, not only with the myriad of available tools, but also with factors like the inherent need of their integration and the deployment of the adequate infrastructure for their operation. The results achieved during project execution indicate that the proposed system, glimpsed as an eventual institutional service or even as one of smaller scope, might become an end-user's ally in the NGS data manipulation.
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Identification de nouveaux gènes de prédisposition héréditaire au cancer du sein par génotypage tumoral et séquençage de nouvelle génération / Identification of new breast cancer susceptibility genes by tumor single nucleotide polymorphism array and next generation sequencingBubien, Virginie 12 December 2016 (has links)
5 à 10% des cancers du sein sont héréditaires mais parmi ceux-ci seulement la moitié est expliquée par une altération constitutionnelle d’un gène de prédisposition connu tels que les gènes BRCA1 et BRCA2. L’importante hétérogénéité génétique qui caractérise les famillesBRCAx rend difficile la réalisation d’études familiales groupées et ne permet pas l’identification de nouveaux gènes de prédisposition au cancer du sein selon les méthodes classiques de liaison génétique ou d’association. Les techniques de séquençage de nouvelle génération (NGS) à l’échelle de l’exome ou du génome entier, autorisent en revanche l’étude de familles individuelles à la recherche de mutations constitutionnelles privées mais le nombre considérable de variants génétiques identifiés impose leur tri sur des critères de pathogénicité ou de récurrence. Un autre critère de tri peut être représenté par l’identification de régions candidates définies en fonction de réarrangements génomiques tumoraux communs à plusieurs tumeurs au sein d’une même famille. Le génotypage tumoral par puces SNP (pour single nucleotide polymorphism) permet en effet la détection d’haplotypes conservés dans des régions récurrentes de LOH (pour loss of heterozygosity) communes à plusieurs tumeurs familiales et donc l’identification de régions candidates suspectes d’abriter des mutations germinales dans des gènes de prédisposition au cancer. La combinaison de ces deux approches, génotypage tumoral puis NGS, a été appliquée à une série de 17 familles avec agrégation de cancers du sein pour lesquelles au moins deux échantillons tumoraux étaient disponibles. Aucun nouveau gène de prédisposition au cancer du sein n’a été identifié mais une mutation délétère constitutionnelle du gène ATM a ainsi été retrouvée, associée à une perte de l’allèle sauvage dans les 2 tumeurs d’une famille BRCAx. L’analyse de 17 tumeurs du sein supplémentaires provenant de 10 familles avec agrégation de cancers du sein et mutation constitutionnelle du gène ATM identifiée chez le cas index, a révélé que l’allèle sauvage d’ATM était fréquemment perdu dans ces tumeurs (>80% contre 20% attendu en situation sporadique ; p<0.001). Ce résultat plaide fortement en faveur de l’implication d’ATM dans la carcinogénèse de ces cancers du sein tel un gène suppresseur de tumeur et suggère que les mutations constitutionnelles d’ATM sont impliquées dans des formes familiales de cancer du sein. / Hereditary breast cancers (BCs) account for 5-10% of all diagnosed BCs, yet only 50% of such tumors arise in the context of a germline mutation in known tumor suppressor genes such as BRCA1 or BRCA2. The vast genetic heterogeneity which characterizes BRCAx families makes grouped studies impossible to perform. Next generation sequencing (NGS) techniques, however, allow individual families to be studied in order to identify private mutations. Single nucleotide polymorphism (SNP) arrays allow the detection of conserved haplotypes within recurrent regions of loss of heterozygosity, common to several familial tumors, therefore identifying genomic loci likely to harbor a germline mutation in cancer predisposition genes. The combination of both exome sequencing and SNP arrays for a series of 17 familial BC did not allow the identification of a novel BC predisposition gene, but revealed a germline ATM mutation associated with a loss of the wild-type allele in a BRCAx family. The analysis of 17 additional breast tumors from ten BC families in which a germline ATM mutation had been identified revealed a high frequency of wild-type allele loss in these tumors (>80% compared to the 20% expected in sporadic BC; p <0.001). This result argues strongly in favor of the involvement of ATM in the carcinogenesis of these tumors as a tumor suppressor gene and suggests that germline ATM mutations are involved in a subset of familial BC.
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Functional analysis of genomic variations associated with emerging artemisinin resistant P. falciparum parasite populations and human infecting piroplasmida B. microti / Analyse fonctionnelle des variations du génome au sein de populations de P. falciparum résistantes à l’artémisinine et chez le piroplasme responsable de la babésiose humaine B. microtiDwivedi, Ankit 28 September 2016 (has links)
Le programme d’élimination du paludisme de l’OMS est menacé par l’émergence etla propagation potentielle de parasites de l’espèce Plasmodium falciparum résistants à l’artémisinine. Récemment il a été montré que (a) des SNPs dans une région du chromosome 13 subissaient une forte sélection positive récente au Cambodge,(b) plusieurs sous-populations de parasites de P. falciparum résistants et sensibles à l’artémisinine étaient présentes au Cambodge, (c) des mutations dans le domaine Kelch du gène k13 sont des déterminants majeurs de la résistance à l’artémisinine dans la population parasitaire cambodgien et (d) des parasites de sous-populations du nord du Cambodge près de la Thaïlande et du Laos sont résistants à la méfloquine et portent l’allèle R539T du gène de k13.Il est donc nécessaire d’identifier la base génétique de la résistance dans le but de surveiller et de contrôler la transmission de parasites résistants au reste du monde, pour comprendre le métabolisme des parasites et pour le développement de nouveaux médicaments. Ce travail a porté sur la caractérisation de la structure de la population de P. falciparum au Cambodge et la description des propriétés métaboliques des sous-populations présentes ainsi que des flux de gènes entre ces sous-populations. Le but est d’identifier les bases génétiques associées à la transmission et l’acquisition de résistance à l’artémisinine dans le pays.La première approche par code-barre a été développée pour identifier des sous-populations à l’aide d’un petit nombre de loci. Une approche moléculaire de PCR-LDR-FMA multiplexée et basée sur la technologie LUMINEX a été mise au point pour identifier les SNP dans 537 échantillons de sang (2010 - 2011) provenant de 16centres de santé au Cambodge. La présence de sous-populations le long des frontières du pays a été établie grâce à l’analyse de 282 échantillons. Les flux de gènes ont été décrits à partir des 11 loci du code-barre. Le code-barre permet d’identifier les sous-populations de parasites associées à la résistance à l’artémisinine et à la méfloquine qui ont émergé récemment.La seconde approche de caractérisation de la structure de la population de P.falciparum au Cambodge a été définie sur la base de l’analyse de 167 génomes de parasites (données NGS de 2008 à 2011) provenant de quatre localités au Cambodge et récupérés à partir de la base de données ENA. Huit sous-populations de parasites ont pu être décrites à partir d’un jeu de 21257 SNPs caractérisés dans cette étude. La présence de sous-populations mixtes de parasite apparait comme un risque majeur pour la transmission de la résistance à l’artémisinine. L’analyse fonctionnelle montre qu’il existe un fond génétique commun aux isolats dans les populations résistantes et a confirmé l’importance de la voie PI3K dans l’acquisition de la résistance en aidant le parasite à rester sous forme de stade anneau.Nos résultats remettent en question l’origine et la persistance des sous-populations de P. falciparum au Cambodge, fournissent des preuves de flux génétique entre les sous-populations et décrivent un modèle d’acquisition de résistance à l’artémisinine.Le processus d’identification des SNPs fiables a été ensuite appliqué au génome de Babesia microti. Ce parasite est responsable de la babésiose humain (un syndrome de type malaria) et est endémique dans le nord-est des Etats-Unis. L’objectif était de valider la position taxonomique de B. microti en tant que groupe externe aux piroplasmes et d’améliorer l’annotation fonctionnelle du génome en incluant la variabilité génétique, l’expression des gènes et la capacité antigénique des protéines. Nous avons ainsi identifié de nouvelles protéines impliquées dans les interactions hôte-parasite. / The undergoing WHO Malaria elimination program is threatened by the emergenceand potential spread of the Plasmodium falciparum artemisinin resistant parasite.Recent reports have shown (a) SNPs in region of chromosome 13 to be understrong recent positive selection in Cambodia, (b) presence of P. falciparum parasiteresistant and sensitive subpopulations in Cambodia, (c) the evidence that mutationsin the Kelch propeller domain of the k13 gene are major determinants ofartemisinin resistance in Cambodian parasite population and (d) parasite subpopulations in Northern Cambodia near Thailand and Laos with mefloquine drugresistance and carrying R539T allele of the k13 gene.Identifying the genetic basis of resistance is important to monitor and control thetransmission of resistant parasites and to understand parasite metabolism for the development of new drugs. This thesis focuses on analysis of P. falciparum population structure in Cambodia and description of metabolic properties of these subpopulations and gene flow among them. This could help in identifying the genetic evidence associated to transmission and acquisition of artemisinin resistance over the country.First, a barcode approach was used to identify parasite subpopulations using smallnumber of loci. A mid-throughput PCR-LDR-FMA approach based on LUMINEXtechnology was used to screen for SNPs in 537 blood samples (2010 - 2011) from 16health centres in Cambodia. Based on successful typing of 282 samples, subpopulations were characterized along the borders of the country. Gene flow was described based on the gradient of alleles at the 11 loci in the barcode. The barcode successfully identifies recently emerging parasite subpopulations associated to artemisinin and mefloquine resistance.In the second approach, the parasite population structure was defined based on167 parasite NGS genomes (2008 - 2011) originating from four locations in Cambodia,recovered from the ENA database. Based on calling of 21257 SNPs, eight parasite subpopulations were described. Presence of admixture parasite subpopulation couldbe supporting artemisinin resistance transmission. Functional analysis based on significant genes validated similar background for resistant isolates and revealed PI3K pathway in resistant populations supporting acquisition of resistance by assisting the parasite in ring stage form.Our findings question the origin and the persistence of the P. falciparum subpopulations in Cambodia, provide evidence of gene flow among subpopulations anddescribe a model of artemisinin resistance acquisition.The variant calling approach was also implemented on the Babesia microti genome.This is a malaria like syndrome, and is endemic in the North-Eastern USA. Theobjective was to validate the taxonomic position of B. microti as out-group amongpiroplasmida and improve the functional genome annotation based on genetic variation, gene expression and protein antigenicity. We identified new proteins involved in parasite host interactions.
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Apport du séquençage haut débit dans l'amélioration de la prise en charge des maladies monogéniquesLacoste Deixonne, Caroline 12 December 2016 (has links)
La diffusion du séquençage haut débit (ou NGS pour Next Generation Sequencing) représente un tel changement d’échelle par rapport aux méthodes classiques de séquençage que les indications et l’organisation du diagnostic moléculaire s’en trouvent profondément modifiées. Le NGS permet à la fois de raccourcir le temps d’analyse et de rendu de résultat et d'élargir considérablement le nombre de gènes testés. Il promet donc d’augmenter la proportion de diagnostics posés et de faciliter l'identification de nouveaux variants et de nouveaux gènes impliqués en pathologie. Cependant dans tous les cas, il génère une quantité de données importante, données qui doivent être analysées et interprétées à l’aide d’outils bioinformatiques spécifiques.Dans la première partie de ce travail, les stratégies existantes ainsi que les difficultés et les enjeux du séquençage haut débit pour le diagnostic moléculaire des maladies génétiques sont discutés. Dans la deuxième partie, la mise en place et la validation technique de cette approche diagnostique sont décrites au sein du laboratoire de Génétique Moléculaire de la Timone à Marseille et illustrées par trois exemples concrets de diagnostics moléculaires posés grâce à la technique de séquençage à haut débit. Dans le domaine spécifique des maladies rares, ces nouvelles technologies sont porteuses d’un réel espoir pour les patients atteints de maladie génétique, permettant d'améliorer globalement leur prise en charge et d'accélérer les progrès dans le domaine de la recherche. / The diffusion of Next Generation Sequencing (NGS) technologies induces an important change that modifies molecular diagnostics indications and prompts laboratories to re-think their diagnostic strategies, up-to-now based on Sanger sequencing routine. Several high throughput approaches are available from the sequencing of a gene panel, to a whole exome, or even a whole genome. In all cases, a tremendous amount of data are generated, that have to be filtered, interpreted and analyzed by the use of powerful bioinformatics tools.In part 1, existing strategies and the difficulties and challenges of high-throughput sequencing for molecular diagnosis in genetic diseases are discussed. In part 2, the set up and the technical validation of this diagnostic approach in the Molecular Genetics’ Laboratory of the Timone Hospital in Marseille is presented and illustrated by 3 examples of complex diagnostics solved thanks to NGS. NGS promises to shorten significantly the time of analysis and results reporting, and to expand the number of tested genes. It also promises to increase the proportion of positive diagnoses. Finally, the NGS can identify new variants and new genes involved in human pathology, thus will globally improve patient clinical care.
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L'analyse de données génomiques et l'annotation à l'heure des NGS : la bioinformatique 2.0 / Genomic data analysis and annotation in the NGS era : Bioinfomatics 2.0Paganini, Julien 15 December 2015 (has links)
Les récents progrès technologiques en termes de séquençage de données génomiques ont entraîné une forte croissance des données disponibles et l'apparition de nouveaux besoins. Initialement limitée à l'analyse de petite quantité de données, la bioinformatique a dû s'adapter à ce nouveau contexte technologique et scientifique afin de répondre aux nouveaux challenges proposés. Par l'intermédiaire de différents projets réalisés dans des contextes différents, cette thèse s'intègre dans ce changement contextuel où la bioinfomatique n'est plus limitée à l'utilisation successive d'outils à objectifs unitaire entrecoupée d'étapes humaine dépendantes. Focalisés sur le développement de stratégies d'analyse complexes pour le développement ou la mise à disposition d'outils entièrement automatisés et la production de données à haute valeur ajoutée, ces travaux permettent de comprendre le rôle important de la bioinformatique 2.0. Ainsi nous montrerons comment elle doit être à même de répondre à des objectifs précis par l'intermédiaire de stratégies intégrant les concepts de la biologie, les outils bioinformatiques existants et l'expertise humaine associée au domaine. En conclusion nous discuterons du nouveau rôle et de l'impact futur de la bioinformatique 2.0 qui requiert une expertise tant sur le plan biologique qu'informatique adaptée aux données NGS. / Recent technological advances in terms of genomic sequencing data led to a strong growth of available data and the emergence of new needs. Initially limited to the analysis of simple sequence or limited amount of data, bioinformatics has to adapt to this new technological and scientific context to meet the new challenges offered. Through different projects in different genomic era, this thesis fits into this contexts change where bioinfomatics is no longer limited to the use of tool with unitary goal and human dependent steps. Focused on the development of complex analysis strategies for the development or the availability of fully automated tools and high-value data, this work introduce the important role of bioinformatics version 2.0. We will show how it is able to answer to precise biological question through specific strategy that integrate all the biological concepts, existing bioinformatics tools and human expertise related to the domain. To conclude, we discuss about the role and the impact of the bioinformatics 2.0 that requires a expert vision at biological and computers level adapted to NGS data.
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Etude de la domestication et de l’adaptation de l’igname (Dioscorea spp) en Afrique par des approches génomiques / Study of the domestication and adaptation of yams (Dioscorea spp) in Africa using genomic approachesAkakpo, Roland 16 May 2018 (has links)
L’igname (Dioscorea spp) est un aliment de base de plus de 100 millions de personnes en Afrique. L’objectif de cette thèse était d'étudier la diversité génomique de l'igname, comprendre les bases génétiques de sa domestication, et d'étudier son adaptation à différentes zones climatiques. L’étude du processus de domestication de l’igname a été menée par une approche de génomique comparée entre l’espèce cultivée D. rotundata et deux espèces sauvages apparentées D. praehensilis et D. abyssinica, en utilisant des données de séquençage NGS génomique. Nous avons mis en évidence des sélections fortes de gènes de la voie de biosynthèse de l’amidon. Des gènes impliqués dans la morphologie des tubercules ou l’aptitude au phototropisme, ainsi que des gènes du complexe NADH deshydrogenase ont également été identifiés comme sélectionnés durant la domestication. Ce même complexe NADH-DH a également été identifié lors de la recherche de gènes associés à la distribution d’une collection d’ignames selon la variabilité climatique. Nous avons aussi créé la première banque de novo d’éléments transposables (ET) de l’igname. L’étude que nous avons menée sur les éléments répétés (ER) du génome de l’igname nous a permis d’identifier une forte corrélation entre la variabilité des abondances relatives d’un grand nombre d’ERs et la variabilité climatique. Enfin, nous avons pu proposer une hypothèse quant à l’origine de l’igname cultivée D. rotundata. La domestication de l'igname dériverait de l'espèce inféodée au milieu forestier, D. praehensilis. Ces résultats remettent en cause l’hypothèse d’une origine stricte en zone de savane pour les espèces cultivées et l’agriculture en Afrique de l'Ouest. / Yam (Dioscorea spp) is a major staple for more than 100 million people in Africa. The main objectives of the present PhD project were to study yam genomic diversity, its domestication, and to characterize the genomic determinism of its adaptation to different climatic zones. We investigated the genetic basis of yam domestication in a comparative genomic approach between the cultivated species D. rotundata and two wild close relatives D. praehensilis and D. abyssinica, by exploiting NGS sequencing data. We demonstrated that genes from the starch biosynthesis were selected during yam domestication. Genes related to tuber morphology or phototropism ability, as well as genes of the NADH dehydrogenase complex were also under selection. The same NADH-DH complex was also identified when assessing adaptation to climate variability. We also created the first de novo database of yam transposable elements (TEs). The study we performed on these repeat elements (REs) highlighted a strong correlation between the variability in relative abundances of numerous REs and climatic variability. Finally, we were able to propose an hypothesis on the origin of the cultivated yam D. rotundata. Our hypothesis identifies the origin of yam in the forest areas, with the species D. praehensilis as the putative progenitor. Our results question the generally admitted hypothesis of savannah origins for crops and agriculture in Africa.
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Determination Of The Gene Networks Controlling Sex Determination In Cucurbitaceae / Détermination des réseaux de gènes contrôlant la détermination du sexe chez les cucurbitacéesAbou Choucha, Fadi 22 June 2018 (has links)
La molécule de l’éthylène (C2H4) est le régulateur principal du sexe chez les cucurbites. Essentiellement, l’éthylène est connu pour son rôle promoteur dans le développement des carpelles et un rôle inhibiteur des étamines dans les fleurs du melon. L’interaction entre les biosynthétique gènes de l’éthylène (CmACS7, CmACS11, et CmACO3) et le facteur de transcription CmWIP1 détermine différentes formes du sexe chez le melon. Le rôle de ces gènes est bien étudié chez le melon. Cependant, le mécanisme qui contrôle l’initiation et la coordination de formation des étamines et des carpelles dans la fleur reste ambigu. En reposant sur l’importance de l’éthylène dans l’expression du sexe chez le melon, j’ai focalisé sur l’identification des gènes impliqués dans la voie signalisation éthylène-sexe. Au cours de la thèse, le criblage des mutants altérés dans la réponse à l’exogène éthylène nous facilitait d’identifier des nouveaux gènes impliqués dans la détermination du sexe chez la famille de Cucurbitacée. Pendant ma thèse j’ai isolé plus de 10 mutants insensibles à l’éthylène de différentes populations du melon. Deux mutants ont été isolés de deux populations monoïques indépendantes. Ces deux mutants provoquent une transition partielle et complète au melon andromonoïque dans la génération M2, respectivement. Un de ces deux mutants a été identifié et caractérisé. Deux autres mutants gsn106 et vat233 ont été criblés de deux populations andromonoïque, provoquent une transition complète et partielle à androïque melon, respectivement. En utilisant le séquençage à haute débit et les analyses génétiques j’ai essayé de cloner et caractériser ces gènes mutants. Par ailleurs, des autres mutants insensibles à l’éthylène sont en cours d’être phénotypes pour le phénotype du sexe. L’isolation et caractérisation des nouveaux gènes impliqués dans le déterminisme du sexe nous aidera pour mettre en place un model clair explant comment le sexe est contrôlé chez les plantes. / Ethylene (C2H4) is an important phytohormone in plants and the main sex regulator in the family Cucurbitaceae. As known, the ethylene promotes the carpel development and inhibits the stamens in the melon flower (Cucumis melo L.). The interplay of the biosynthesis genes (CmACS7, CmACS11, et CmACO3) and the transcription factor CmWIP1 generates different sexual forms in melon. The role of these genes in the sex expression is well studied. However, the mechanism that controls the initiation and coordination of stamen and carpel development in the flower remains ambiguous. Based on the importance of the ethylene in the sex determination, I aimed to isolate novel genes involved in the pathway ethylene-sex in the melon (Cucumis melo L.). For this purpose, I used the response to exogenous ethylene in the etiolated seedlings (known as the triple response phenotype) to isolate ethylene-insensitive mutants. During my thesis I isolated more than 10 ethylene-insensitive mutants from six EMS-mutagenised melon populations. Some of these mutants induced changes in the sex expression of the melon. . Two mutants were isolated from two independent monoecious populations (female and male flowers on the same plant) and induced a partial and a complete sexual transition to the andromonoecious melon in the second generation M2, respectively. One of them was cloned and characterized using Omics tools. Two other mutants (gsn106) and (vat233) screened from two independent andromonoecious melon (bisexual and male flowers on the same plant) populations, induced complete and partial sexual transitions into androecy (only male flowers), respectively. Using Next-Generation Sequencing (NGS) and the genetic analysis, we are trying to clone and characterise these mutants (gsn106) and (vat233). In the same way, we continue to observe others promising ethylene-insensitive mutants (vat306, vat175, and vat230) for the sex phenotype. The isolation and characterisation of novel genes involved in the sex determination will permit to provide a new and clear model explains of the sex determination mechanism in plants.
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Efektivní hledání překryvů u NGS dat / Effective Search for Overlaps in NGS DataMatocha, Petr January 2017 (has links)
The main theme of this work is the detection of overlaps in NGS data. The work contains an overview of NGS sequencing technologies that are the source of NGS data. In the thesis, the problem of overlapping detection is generally defined. Next, an overview of the available algorithms and approaches for detecting overlaps in NGS data is created. Principles of these algorithms are described herein. In the second part of this work a suitable tool for detecting approximate overlaps in NGS data is designed and its implementation is described herein. In conclusion, the experiments performed with this tool and the conclusions that follow are summarized and described.
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