A kinetic model of electronic energy migration within pairs of photophysically non-identical fluorophores has been developed. The model applies to fluorescent groups that exhibit different photophysical and spectral properties when attached to different positions in a macromolecule. The energy migration within such asymmetric pairs is partially reversible, which leads to the case of partial donor-donor energy migration (PDDEM). The model of PDDEM is an extension of the recently developed donor-donor energy migration model (DDEM, F. Bergström et al, PNAS 96 (1999) 12477), and applies to quantitative measurements of energy migration rates and distances within macromolecules. One important distinction from the DDEM model is that the distances can be obtained from fluorescence lifetime measurements. A model of fluorescence depolarisation in the presence of PDDEM is also presented. To experimentally test the PDDEM approach, different model systems were studied. The model was applied to measure distances between rhodamine and fluorescein groups within on-purpose synthesised molecules that were solubilised in lipid bilayers. Moreover, distances were measured between BODIPY groups in mutant forms of the plasminogen activator inhibitor of type 2 (PAI-2). Measurements of both the fluorescence intensity decays and the time-resolved depolarisation were performed. The obtained distances were in good agreement with independent determinations. Finally, the PDDEM within pairs of donors is considered, for which both donors exhibit a nonexponential fluorescence decay. In this case it turns out that the fluorescence relaxation of a coupled system contains distance information even if the photophysics of the donors is identical. It is also demonstrated that the choice of relaxation model has a negligible effect on the obtained distances. The latter conclusion holds also for the case of donor-acceptor energy transfer.

This thesis concerns the development and characterisation of X-ray imaging systems based on single photon processing. “Colour” X-ray imaging opens up new perspectives within the fields of medical X-ray diagnosis and also in industrial X-ray quality control. The difference in absorption for different “colours” can be used to discern materials in the object. For instance, this information might be used to identify diseases such as brittle-bone disease. The “colour” of the X-rays can be identified if the detector system can process each X-ray photon individually. Such a detector system is called a “single photon processing” system or, less precise, a “photon counting system”. With modern technology it is possible to construct photon counting detector systems that can resolve details to a level of approximately 50 µm. However with such small pixels a problem will occur. In a semiconductor detector each absorbed X-ray photon creates a cloud of charge which contributes to the image. For high photon energies the size of the charge cloud is comparable to 50 µm and might be distributed between several pixels in the image. Charge sharing is a key problem since, not only is the resolution degenerated, but it also destroys the “colour” information in the image. This thesis presents characterisation and simulations to provide a detailed understanding of the physical processes concerning charge sharing in detectors from the MEDIPIX collaboration. Charge summing schemes utilising pixel to pixel communications are proposed. Charge sharing can also be suppressed by introducing 3D-detector structures. In the next generation of the MEDIPIX system, Medipix3, charge summing will be implemented. This system, equipped with a 3D-silicon detector, or a thin planar high-Z detector of good quality, has the potential to become a commercial product for medical imaging. This would be beneficial to the public health within the entire European Union. / Denna avhandling berör utveckling och karaktärisering av fotonräknande röntgensystem. ”Färgröntgen” öppnar nya perspektiv för medicinsk röntgendiagnostik och även för materialröntgen inom industrin. Skillnaden i absorption av olika ”färger” kan användas för att särskilja olika material i ett objekt. Färginformationen kan till exempel användas i sjukvården för att identifiera benskörhet. Färgen på röntgenfotonen kan identifieras om detektorsystemet kan detektera varje foton individuellt. Sådana detektorsystem kallas ”fotonräknande” system. Med modern teknik är det möjligt att konstruera fotonräknande detektorsystem som kan urskilja detaljer ner till en upplösning på circa 50 µm. Med så små pixlar kommer ett problem att uppstå. I en halvledardetektor ger varje absorberad foton upphov till ett laddningsmoln som bidrar till den erhållna bilden. För höga fotonenergier är storleken på laddningsmolnet jämförbar med 50 µm och molnet kan därför fördelas över flera pixlar i bilden. Laddningsdelning är ett centralt problem delvis på grund av att bildens upplösning försämras, men framför allt för att färginformationen i bilden förstörs. Denna avhandling presenterar karaktärisering och simulering för att ge en mer detaljerad förståelse för fysikaliska processer som bidrar till laddningsdelning i detektorer från MEDIPIX-projekter. Designstrategier för summering av laddning genom kommunikation från pixel till pixel föreslås. Laddningsdelning kan också begränsas genom att introducera detektorkonstruktioner i 3D-struktur. I nästa generation av MEDIPIX-systemet, Medipix3, kommer summering av laddning att vara implementerat. Detta system, utrustat med en 3D-detektor i kisel, eller en tunn plan detektor av högabsorberande material med god kvalitet, har potentialen att kunna kommersialiseras för medicinska röntgensystem. Detta skulle bidra till bättre folkhälsa inom hela Europeiska Unionen.

This project investigates a laser radar system. The system is based on the principles of time-correlated single photon counting, and by measuring the times-of-flight of reflected photons it can find range profiles and perform three-dimensional imaging of scenes. Because of the photon counting technique the resolution and precision that the system can achieve is very high compared to analog systems. These properties make the system interesting for many military applications. For example, the system can be used to interrogate non-cooperative targets at a safe distance in order to gather intelligence. However, signal processing is needed in order to extract the information from the data acquired by the system. This project focuses on the analysis of different signal processing methods. The Wiener filter and the Richardson-Lucy algorithm are used to deconvolve the data acquired by the photon counting system. In order to find the positions of potential targets different approaches of non-linear least squares methods are tested, as well as a more unconventional method called ESPRIT. The methods are evaluated based on their ability to resolve two targets separated by some known distance and the accuracy with which they calculate the position of a single target, as well as their robustness to noise and their computational burden. Results show that fitting a curve made of a linear combination of asymmetric super-Gaussians to the data by a method of non-linear least squares manages to accurately resolve targets separated by 1.75 cm, which is the best result of all the methods tested. The accuracy for finding the position of a single target is similar between the methods but ESPRIT has a much faster computation time.

Les propulseurs électriques sont de plus en plus utilisés pour des missions de correction de trajectoire des satellites et pourront dans un avenir proche être utilisés pour le transfert d’orbite. Ces propulseurs constituent une excellente alternative aux propulseurs chimiques grâce à leur rendement élevé et une substantielle économie de carburant réalisée par rapport aux propulseurs chimiques. Les propulseurs à effet Hall créent la poussée par l’accélération d’ions positifs de xénon ou de krypton dans un plasma confiné par un champ magnétique. L’objet de ce manuscrit concerne principalement les caractéristiques de l’accélération des ions et des atomes dans un propulseur à effet Hall. Les influences de la largeur du canal de décharge, de la topologie magnétique et de la cathode sur l’efficacité d’accélération des ions sont étudiées. Des pistes d’optimisation de l’architecture du propulseur sont alors proposées qui pourraient être particulièrement avantageuses sur les propulseurs de petite taille, comme l’élargissement du canal et l’augmentation du champ magnétique près des parois du canal. L’influence de la position et du potentiel de la cathode sur la déviation du faisceau ionique est révélée. L’évolution temporelle basse fréquence du champ électrique est mesurée par comptage synchrone de photons et suggère que la température atomique joue un rôle important dans les oscillations basse fréquence de la décharge. Par ailleurs, l’influence du champ magnétique sur les performances d’un propulseur proche des modèles de vol a été mesurée grâce à l’utilisation d’un moteur doté d’une topologie magnétique flexible. Ceci a montré la difficulté de définir un paramètre numérique capable de synthétiser l’information complexe de la répartition spatiale du champ magnétique dans le canal de décharge. Les très faibles modifications des performances par le champ magnétique soulignent l’importance de la précision dans la mesure. / Electric propulsion systems are more and more often used for trajectory correction of satellites and may soon be used for orbit transfer. These devices represent a great alternative to classic chemical propulsion devices thanks to their high efficiency and propellant mass savings. Hall effect thruster provide thrust by the acceleration of xenon or krypton ions in a magnetized confined plasma. The study presented in this manuscript mainly addresses characteristics of ion and atom acceleration in a Hall effect thruster. Influence of channel width, magnetic topology and cathode parameters on ion acceleration efficiency is investigated. Ways to optimize thruster architecture are suggested that may be particularly relevant for low power thrusters, such as widening thruster channel and increasing magnetic field amplitude near channel walls. Influence of cathode position with respect to the thruster channel exit plane and its potential with respect to ground on ion beam deviation has been revealed with two thrusters. Low frequency time evolution of the accelerating electric field was measured using lock-in photon counting system. Results strongly suggest that the atom temperature plays a crucial role in low frequency time evolution of the whole plasma discharge. Measurement of performances as a function of the magnetic field demonstrated that numeric parameters are compulsory to carry on a relevant parametric study. These parameters would summarize the 2D information of magnetic topology. Weak influence of magnetic topology revealed that thrust measurement precision needs to be increased by at least one order of magnitude if one wants to reach a better understanding of plasma confinement in a Hall effect thruster.

No description available.

[pt] Dois dispositivos de contagem de fótons únicos, sensíveis a comprimentos de onda no infravermelho próximo e médio, são propostos e experimentalmente investigados. Ambos utilizam uma técnica de dois estágios, composta de uma etapa inicial de conversão de freqüências em um cristal não-linear seguida de detecção por um fotodiodo avalanche de silício. Enquanto o primeiro projeto é voltado à detecção de fótons únicos a 1.55 μm para comunicações quânticas via fibra óptica, usando um processo intra-cavidade, o segundo projeto prevê o desenvolvimento de um contador de fótons operando a 4.65 μm para sistemas de espaço livre. Neste caso, um estudo de viabilidade para um sistema prático de criptografia quântica operando em um comprimento de onda no infravermelho médio é realizado. Os resultados mostram que, usando a tecnologia disponível na atualidade, tal sistema pode ser construído, embora sua utilidade se mostre restrita a enlaces possuindo certas condições meteorológicas específicas. / [en] Two single photon counting devices, operating at near- and mid-infrared wavelengths, are introduced and experimentally investigated. Both use a twostage technique, comprised of an initial frequency up-conversion procedure inside a nonlinear crystal followed by a silicon avalanche photodiode. Whereas the first project consists on detection of single photons at 1.55 ìm for fiber-optic-based quantum communications, using a cavity-enhanced procedure, the second project envisions the development of a single-photon counter operating at 4.65 ìm for free-space systems. In this case, a feasibility study for a practical quantum key distribution system operating in a mid-infrared wavelength is performed. The results show that, using present-day technology, such a system can be constructed, albeit its usefulness would be restricted to operation under very specific weather conditions.

No description available.

During the past years photon-counting detectors (PCDs) have emerged as an alternative to conventional energy-integrating detectors and may significantly improve the standard of care for computed tomography (CT). There are two main alternatives for the material of the detector: cadmium telluride (CdTe) and silicon (Si). The settings of the X-ray tube and the applied filters need to be evaluated and optimized for the new detector technology. In this report, Monte Carlo simulations are used to determine how image quality is affected by different X-ray tube voltages and filter thicknesses. The image quality indicators that were chosen to evaluate are detective quantum efficiency (DQE) for material quantification and both DQE and dose-normalized signal-difference-to-noise ratio (SDNR) for detection tasks. Overall, silicon-based detectors performed better than cadmium-based detectors for quantification imaging tasks for all object thicknesses, while cadmium-based detectors were superior for detection imaging tasks in larger patients. For both silicon- and cadmium-based detectors, the dose-normalized image quality was largely independent of filter thickness, while the X-ray tube voltage had a more distinct impact on the result, where low voltages were optimal. / Under de senaste åren har fotonräknande detektorer blivit aktuellt som ett alternativ till konventionella energiintegrerande detektorer och kommer troligen förbättra datortomografibilder avsevärt. För de nya detektorerna finns det två huvudsakliga materialalternativ: kadmiumtellurid (CdTe) och kisel (Si). Inställningarna för röntgenröret och det pålagda filtret behöver utvärderas och optimeras för den nya detektorteknologin. I denna rapport användes Monte Carlo-simuleringar för att bestämma hur bildkvaliteten påverkades av rörspänningen och filtertjockleken. Bildkvaliteten bestämdes sedan utifrån tre indikatorer, detective quantum efficiency (DQE) för materialbestämning samt både DQE och dosnormaliserad signal-difference-to-noise ratio (SDNR) för detektionsuppgifter.    Den kiselbaserade detektorn presterade bättre än den kadmiumbaserade för materialbestämning för alla patientstorlekar medan den kadmiumbaserade presterade bättre på detektionsuppgifterna för större patienter. Vidare var den dosnormaliserade bildkvaliteten för både kisel- och kadmiumdetektorer svagt beroende av filtertjocklek medan båda påverkades starkt av rörspänningen, där låga spänningar var att föredra.

No description available.

Time-Resolved diffuse optics is a powerful and safe technique to quantify the optical properties (OP) for highly scattering media such as biological tissues. The OP values are correlated with the compositions of the measured objects, especially for the tissue chromophores such as hemoglobin. The OP are mainly the absorption and the reduced scattering coefficients that can be quantified for highly scattering media using Time-Resolved Diffuse Optical Spectroscopy (TR-DOS) systems. The OP can be retrieved using Time-Resolved Diffuse Optical Imaging (TR-DOI) systems to reconstruct the distribution of the OP in measured media. Therefore, TR-DOS and TR-DOI can be used for functional monitoring of brain and muscles, and to diagnose some diseases such as detection and localization for breast cancer and blood clot. In general, TR-DOI systems are non-invasive, reliable, and have a high temporal resolution. TR-DOI systems have been known for their complexity, bulkiness, and costly equipment such as light sources (picosecond pulsed laser) and detectors (single photon counters). Also, TR-DOI systems acquire a large amount of data and suffer from the computational cost of the image reconstruction process. These limitations hinder the usage of TR-DOI for widespread potential applications such as clinical measurements. The goals of this research project are to investigate approaches to eliminate two main limitations of TR-DOI systems. First, building TR-DOS systems using custom-designed free-running (FR) and time-gated (TG) SPAD detectors that are fabricated in low-cost standard CMOS technology instead of the costly photon counting and timing detectors. The FR-TR-DOS prototype has demonstrated comparable performance (for homogeneous objects measurements) with the reported TR-DOS prototypes that use commercial and expensive detectors. The TG-TR-DOS prototype has acquired raw data with a low level of noise and high dynamic range that enable this prototype to measure multilayered objects such as human heads. Second, building and evaluating TR-DOI prototype that uses a computationally efficient algorithm to reconstruct high quality 3D tomographic images by analyzing a small part of the acquired data. This work indicates the possibility to exploit the recent advances in the technologies of silicon detectors, and computation to build low-cost, compact, portable TR-DOI systems. These systems can expand the applications of TR-DOI and TR-DOS into several fields such as oncology, and neurology. / Thesis / Doctor of Philosophy (PhD)