Rupture of the anterior cruciate ligament (ACL) of the knee leads to chronic joint instability and reduced range of motion while the long term results are marred by a high prevalence of degenerative joint disease especially osteoarthritis. Bundles of collagen threads have been widely investigated for the repair of torn ACL, but are limited by insufficient tissue ingrowth to repopulate and completely regenerate these grafts. We have developed a novel in vitro method of characterizing fiber-based thread matrices by probing their ability to promote tissue ingrowth from a wound margin as a measure of their ability to promote repopulation and regeneration. This method is useful in the optimization of thread scaffolds, and is sensitive enough to distinguish between subtle variations in biopolymer chemistry and organization. Furthermore, this method was used to characterize the effects of crosslinking on the cell outgrowth and correlated the findings with the mechanical properties of collagen threads. The results suggest that crosslinking is required to achieve sufficient mechanical properties for high stress applications such as ACL replacement, but regardless of technique, crosslinking attenuated the cell outgrowth properties of the threads. To improve the regenerative capacity of these scaffolds, novel fibrin microthread matrices were developed with a similar morphology to collagen threads and sufficient mechanical strength to be incorporated in composite thread scaffold systems. These fibrin microthreads were loaded with FGF-2, a potent mitogen and chemotactic agent that works synergistically with fibrin in regulating cell signaling and gene expression. Increases in fibroblast migration and proliferation in FGF-2-loaded fibrin threads were successfully demonstrated with the concomitant promotion of oriented, aligned, spindle-like fibroblast morphology. These results suggest that fibrin-FGF-2 microthreads have distinct advantages as a biomaterial for the rapid regeneration of injured tissues such as the ACL.

Os implantes mamários de silicone têm sido empregados, tanto nas cirurgias de aumento das mamas, quanto na reconstrução do tecido mamário após mastectomia. A segurança biológica dos implantes de silicone merece estudo relacionado aos processos de esterilização empregados, pois podem constituir-se em fator de comprometimento da estrutura química do polímero e, conseqüentemente, da biocompatibilidade. Este estudo consistiu na avaliação da biocompatibilidade de implantes mamários de silicone após terem sido submetidos aos processos de esterilização por calor seco, radiação gama e óxido de etileno. O parâmetro avaliado foi a viabilidade celular, empregando o método de difusão em agar e de captura do vermelho neutro. As amostras compreenderam implantes de silicone gel lisos, texturizados e revestidos com poliuretano e implantes texturizados pré-cheios com solução salina. Também foi realizado o teste de endotoxinas bacterianas pelo método do LAL e determinação da taxa de migração do gel de silicone (teste de bleed). Os três métodos de esterilização mostraram-se igualmente eficientes pela comprovação da condição de esterilidade dos implantes através de metodologia descrita na Farmacopéia Americana 27 edição. Os níveis de endotoxinas bacterianas dos implantes, também atenderam aos requisitos dos compêndios oficiais. Na avaliação da biocompatibilidade todos os implantes, independente dos processos de esterilização utilizados, apresentaram ausência de citotoxicidade. Os resultados do teste de bleed mostraram uma maior taxa de migração de gel para os implantes de superfície lisa em comparação com os implantes de superfície texturizada e revestida com poliuretano, quando esterilizados por calor seco. Ao comparar a taxa de migração do gel para os implantes de superfície lisa esterilizados por calor seco e óxido de etileno, obteve-se uma maior taxa de migração para aqueles implantes esterilizados por óxido de etileno. As diferentes avaliações realizadas neste estudo abrangeram aspectos biológicos, químicos e físicos relevantes para garantir um produto de boa qualidade e que, por assegurar a manutenção da característica de biocompatibilidade, resulta na segurança biológica deste tipo de implante. / Silicone breast implants have been widely used for mammary augmentation and reconstruction surgery. Biological safety of these implants can be damaged by sterilization methods. This study consisted of the biocompatibility assessment of breast implants through cell viability, employing the agar diffusion test and neutral red uptake. Four silicone breast implants were tested: smooth, textured, polyurethane covered silicone gel breast implant and textured saline-filled breast implant. Sterilization methods comprised dry-heat, ethylene oxide and γ-radiation. Detection of bacterial endotoxins employing LAL test and gel bleed was also performed. The three methods of sterilization revealed equally efficacious, through the sterility confirmation of implants employing methodology described in 27th edition of United States Pharmacopeia. At the same way the leveis of bacterial endotoxins of implants accorded with the pharmacopeial requirements. In theevaluation of biocompatibility ali the implants, independently of the sterilization process used, showed no cytotoxicity signals. The results of gel bleed revealed a higher migration rate from the smooth implants in comparison with the textured and polyurethane-covered implants sterilized by dry-heat. When was compared the gel migration of smooth implants sterilized by dry-heat and ethylene oxide, was obtained a higher rate of gel bleed of those implants sterilized by ethylene oxide. The different evaluations performed in this study comprised biological, chemical and physical aspects, that are relevant to assure a good quality product and by having maintained the characteristics of biocompatibility, resulted in the biological safety on this kind of implant.

Due to the increasing demand from patients and dentists for highly aesthetic and strong, metal-free restorations there has been a rapid increase in research into dental CAD CAM technique and zirconia based restorations over the last decade. Such new technology has the potential to take the place of conventional techniques and materials for fabricating indirect dental restorations in the future. In this PhD thesis, five laboratory studies were designed to investigate zirconia bridges constructed using dental CAD CAM. The studies concentrated on: 1. Ideal force applied by dentists for cementing zirconia bridges and the impact on seating. 2. The effect of firing cycles and zirconia thickness on the fit of zirconia bridges. 3. The effect of span length on the fit of three and four unit all zirconia bridges. 4. The effect of veneering on the strength of three unit zirconia bridges. 5. The fit of three unit all zirconia bridges produced by digital and conventional techniques. For these laboratory studies an ideal three unit (and four unit for study 3) fixed-fixed all ceramic bridge preparation was carried out on two plastic teeth and all SLA models and zirconia based bridges were made using the Lava COS and Lava™ CAD CAM system (3M, ESPE). In addition to the laboratory studies, a clinical audit was carried out to assess satisfaction (dentist, dental technician and patient) with zirconia based restorations (through a xvi series of questionnaires) made and fitted at Dundee Dental Hospital and School. In addition, as part of this audit a simple cost analysis was carried out to explore the differences in cost between zirconia based restorations and high fusing gold alloy based metal ceramic restorations. Four of the studies (studies 1, 2, 3 and 5) investigated the internal and marginal fit of the zirconia based restorations under differing laboratory and clinical procedures and conditions. It was found that the seating force used to cement a zirconia based bridge had no impact upon fit (Study 1). Whilst the thickness of zirconia (all-zirconia bridge and un-veneered zirconia framework) did not affect the fit of the restoration, veneering the framework did lead to a statistically significant deterioration in fit (Study 2). Although leading to a poorer fit veneering did have a positive effect in strengthening the zirconia framework, but neither un-veneered nor veneered frameworks were as strong as monolithic/all zirconia bridges (Study 4). Despite the high shrinkage during post milling sintering and the potential for greater distortion on longer span bridges, the longer span bridges investigated in Study 3 did not impact upon fit. In study 1, 2, 3 and 4 the Lava COS intra-oral scanner was used to create a digital impression of the tooth preparations and study 5 confirmed that the fit of bridges made from these impressions were better than those made using conventional addition cured silicone putty and wash impressions (Study 5). The results of the questionnaires used in the audit revealed high satisfaction rates with all stake holders and the cost analysis showed that producing zirconia based restorations can be five to six times cheaper than conventional gold based restorations. Despite the variations in fit which were found in Studies 2 and 5, all bridges produced were within what would be regarded as clinically acceptable and comparable to those produced with more traditional techniques.

Introdução: A colocação de próteses através da junção esofagogástrica propicia o refluxo gastroesofágico. Objetivos e métodos: Avaliação da paliação da disfagia, manifestações broncoaspirativas, parâmetros nutricionais e qualidade de vida de 30 pacientes com tumores avançados da junção esofagogástrica tratados por prótese metálica auto-expansível com válvula anti-refluxo de 2001 a 2004. Resultados: Melhora da disfagia e regurgitação. Sem alteração na tosse, eructação, pirose, parâmetros nutricionais e qualidade de vida. Conclusões: A prótese com válvula anti-refluxo foi eficaz na paliação da disfagia, impediu a ocorrência de manifestações broncoaspirativas, não interferiu nos parâmetros nutricionais e qualidade de vida / Background: Placement of stents through the gastroesophageal junction can cause reflux. Objective and methods: Evaluate dysphagia palliation, aspiration symptoms, nutritional parameters and quality of life in 30 patients treated for advanced gastroesophageal junction tumors with a self-expanding metal stent with anti-reflux valve from 2001 to 2004. Results: Dysphagia and regurgitation symptoms improved after placement. There was no changes in heartburn, cough, belching symptoms and also no improvement in nutritional parameters or quality of life. Conclusions: Antireflux valve self-expanding stents was effective in dysphagia palliation, avoided aspiration symptoms and did not affect nutritional parameters or quality of life

<p>Over one million hip prostheses are implanted in patients worldwide each year and the need is increasing as the patient group of younger and more active patients is increasing. Many parameters affect the longevity of the implant, where aseptic loosening caused by wear debris is the most common reason for revision. To be able to monitor wear in vivo and also to predict the longevity of new materials for hip prostheses are therefore important issues in this interdisciplinary research area. This thesis comprise a true non-invasive 3D method for determination of acetabular cup wear using Computed Tomography (CT) intended for clinical routine use in order to plan for a revision. It also comprises investigations of the influence of surface roughness and sterilization method in wear testing of materials for hip prostheses. Mainly wear of ultra high molecular weight polyethylene (UHMWPE) was investigated since it is the most common soft bearing in hip prostheses. The 3D-CT method was found to be easy to use and showed an accuracy and repeatability at a clinical relevant level for acetabular cup wear. It should lend itself well to semi-automation. The influence of surface roughness was investigated with both a multidirectional pin-on-disk machine and with a hip simulator. A new low friction coating, Micronite, was also evaluated with the pin-on-disk machine. This coating showed potential for use in artificial joints, but further investigations are needed. In the hip simulator test, it was found that not only a rougher counter surface increased wear, but also sterilization by γ-irradiation increased wear of UHMWPE cups.</p>

Diss. (sammanfattning) Stockholm : Karolinska institutet, 2007. / Härtill 6 uppsatser.

Thesis (MScEng)--Stellenbosch University, 2013. / ENGLISH ABSTRACT: Osteoarthritis (OA), also known as degenerative joint disease is a progressive disorder of the joints caused by gradual loss of cartilage and resulting in the development of bony spurs and cysts at the margins of the joints. The degradation of the musculoskeletal system, which is mainly caused by joint injury, obesity (leading to musculoskeletal fatigue) and aging can also lead to osteoarthritis. The hands, feet, spine, and large weight-bearing joints, such as the hips and knees are commonly affected. The only medical solution to severe cases of osteoarthritis is the surgical reconstruction or replacement of a malformed or degenerated joint, better known as arthroplasty. Arthroplasty makes use of biomedical implants and replacements to restore functionality of the joints. Biomedical engineering in arthroplasty is an ever increasing field of interest as a result of its innovative improvements to surgical quality. Certain cases of partial osteoarthritis require less surgical action. Partial knee replacement surgery, also known as unicondylar (or unicompartmental) knee arthroplasty involves a covering which is placed over the affected area to resurface the affected bone and protect the patient from further degeneration. Advantages of partial replacement include faster recovery time and less post-operative pain. The biomedical implants used for these operations consist of a standardized implant that is fit onto the bone by modifying (cutting away) the outer structure of the bone. The result is known to cause post-operative discomfort among some patients. The problem with these standard designs includes the requirement of the removal of unaffected (healthy) bone matter, leading to induced trauma and pain for patients during the recovery phase of the operations. A preferred alternative to the standard design would be to create a custom implant for every patient, reducing the need to remove parts of unaffected bone matter. The implementation of this proposed method tends toward Minimally Invasive Surgery (MIS). MIS is normally preferred as it reduces the risk of various negative consequences of normal arthroplasty such as nerve or tendon damage during surgery. It could be argued that the proposed method may cause less damage to the fragile tendon, bloodflow, and nerve networks of the knee. Increasing material costs of metal products introduce great interest in more cost efficient forming processes to reduce the loss of redundant blank material. Incremental Sheet Forming (ISF), a relatively new class of forming process, has the potential to meet the need for this more efficient forming process. The ISF process is highly flexible, can be developed in normal milling machines, and can reduce production cost by up to 90% in comparison to processes such as stamping. The ISF process is a non-patented process, as the existing patents are referred to the designed machines and not the process. The availability of the ISF process contributes greatly to its attractiveness. ISF can be implemented in any facility that has access to a three- or more-axis CNC machine. The advantage of ISF implemented in CNC machines is that CNC technology has already reached a mature stage in development, contributing to the accuracy and methodology (such as feed rate or angular velocity of the tool) of the ISF process. The forming of valuable lightweight materials is well covered by ISF processes. A variety of studies contribute to research on the forming of titanium and titanium based alloys as part of ISF of lightweight materials. The ISF process utilizes the functionality of commercial CNC machines, improving the process availability of many manufacturing companies. The ISF process offers fast setup times and flexibility of the forming process. The purpose of this project is to define a process chain for creating a customized biomedical implant as well as determining the validity of the process chain by applying each step. The design and development procedure of a titanium based biomedical arthroplasty implant using innovative Incremental Sheet Forming (ISF) techniques will be documented, as well as an investigation of the financial cost and potential gain that this implant can offer. / AFRIKAANSE OPSOMMING: Osteoartritis is 'n gewrig siekte wat degeneratiewe newe-effekte behels in die gewrigte. Hierdie siekte lei to die geleidelike verlies van kraakbeen en lei tot die onreelmatige ontwikkeling van abnormale beengroei. Osteoartritis kan ook deur beserings in die gewrig veroorsaak word. Die hande, voete, ruggraat, en enige groter gewigdraende gewrigte, soos die heupe en knieë kan geaffekteer word. Die enigste mediese oplossing tot ernstige gevalle van die siekte is chirurgiese rekonstruksie of vervanging van die gewrig, meer bekend as artroplastie. Artroplastie maak gebruik van biomediese implantate om funksionaliteit van die gewrig te herstel. Biomediese ingenieurswese in artroplastie is 'n toenemende navorsingsveld as gevolg van die innoverende aspekte om chirurgiese kwaliteit te verhoog. Sekere gevalle van gedeeltelike osteoartritis vereis veel minder chirurgiese behandeling. Gedeeltelike knie vervanging chirurgie, meer bekend as unikompartementele knie artroplastie, behels 'n bedekking wat slegs die geaffekteerde been bedek, om die pasiënt van verdere degenerasie te beskerm. Voordele van gedeeltelike vervanging sluit vinniger herstel tyd en minder pyn in. Die biomediese implantate wat gebruik word vir hieride operasies bestaan uit standaard ontwerpe wat aan die been gepas word deur die wysiging (of wegsny) van die buitenste beenstruktuur. Die nagevolg van hierdie chirurgie is lang herstel periodes en kan ongemaklikheid in die knie veroorsaak. Die probleem met die bogenoemde standaard is dat die prosedure die verweidering van selfs ongeaffekteerde (of gesonde) been in sluit, wat lei tot verdere kniepyn en ongemak vir pasiënte lei tydens die herstelperiode. 'n Verkiesde alternatief tot die standaard ontwerpe is om 'n persoonlikke implantaat vir elke pasiënt te skep, en so kan die behoefte om dele van ongeaffekteerde been te behou moontlik wees. Die toepassing van die voorgestelde metode neig na Minimale Skade Chirurgie (MSC). MSC word gewoonlik verkies om die risiko van verskeie negatiewe nagevolge te verminder, en skade aan die tendon, bloed- en senunetwerke van die knie te beperk. Die toenemende materiaalkoste vand metal produkte lei tot 'n groot belangstelling in meer koste besparing vormings prosesse, om sodoende die verlies van oortollige materiaalverlies te verminder. Inkrementele Plaat Vervorming (IPV), 'n relatiewe nuwe klas van vervorming, is 'n waardige kanidaat om hierdie doel te bereik. Die IPV proses is baie toepaslik, en kan deur die gebruik van Rekenaar Numeriese Kontrole (RNK) masjienerie toegepas word. Verder sal dit vervaardigingskoste kan verlaag met soveel as 90% in vergelyking met ander prosese soos die stempel metode. Die beskikbaarheid van die IPV proses dra grootliks by tot die proses se aantreklikheid in die industrie. IPV kan geimplementeer word in enige fasiliteit wat toegang tot 'n drie-as RNK masjien het. Die voordeel van dit is die feit dat RNK masjienerie klaar ontwikkel en volwasse is, wat kan bydra tot goeie akkuraatheid in die vormingsproses. Die vervaardiging van laegewig materiale soos titaan of aluminium is gedokumenteer. 'n Verskeidenheid van studies dra waarde tot navorsing van die vormingsproses van titaan as deel hiervan. Die IPV proses bied vinnige opstel tye en goeie buigsaamheid met die vormingsproses, veral met behulp van 'n vyf-as masjien. Die doel van hierdie projek is om 'n proses ketting te ontwerp. Die proses ketting, wat uit vele stappe bestaan, sal die ontwerp en vervaardigingsproses van 'n persoonlike biomediese knie implantaat bevestig deur middel van die IPV vormings tegniek. Validasie van die proses ketting sal dus plaasvind deur die stappe van die voorgestelde proses ketting uit te voer. 'n Finale ondersoek sal die finansiele en regalutoriese aspekte van die projek aanspreek.

A adaptação passiva e estabilidade têm se tornado os dois pré-requisitos de maior importância na escolha dos materiais e técnicas de confecção em prótese implantossuportada. A proposta deste trabalho foi avaliar, com auxílio dos strain gauges ou extensômetros lineares elétricos, a deformação média ocorrida nos intermediários e na região peri-implantar mediante a instalação da infraestrutura parafusada durante sete etapas de confecção da prótese parcial fixa. Para a investigação, foi utilizado um modelo experimental homogêneo à base de poliuretano com dois implantes hexágono externo paralelos entre si. Sobre os implantes foram acoplados pilares Multi-unit de 5 mm de altura que receberam na sua superfície três strain gauges dispostos de maneira eqüidistante entre si. Também foram colados na superfície superior do bloco experimental quatro strain gauges para cada implante, posicionados nas faces mesial, distal, vestibular e lingual visando medir as tensões nestas áreas. Os testes foram realizados parafusando os corpos de prova sobre os intermediários utilizando-se para isto um dispositivo eletrônico de torque com força máxima de 10 Ncm. As leituras foram realizadas em sete momentos, obtendo os valores médios de deformação no poliuretano e no intermediário respectivamente: 1) cilindros de cobalto-cromo pré-fabricados usinados como grupo controle (36,4µ&#x3B5; e 87,5µ&#x3B5;), 2) infraestrutura sobrefundida em monobloco com liga de níquel-cobalto-cromo (340,3µ&#x3B5; e 131,4µ&#x3B5;), 3) em seguida seccionada com espaço livre para solda (43,2µ&#x3B5; e 66,6µ&#x3B5;), 4) seccionada com espaço para solda preenchida com resina acrílica quimicamente ativada (Duralay) (74µ&#x3B5; e 86,8µ&#x3B5;), 5) seccionada e soldada (280µ&#x3B5; e 146,2µ&#x3B5;), 6) soldada com aplicação de cerâmica (288,7µ&#x3B5; e 151,1µ&#x3B5;) e 7) finalizada com aplicação do glaze, acabamento e polimento (359,9µ&#x3B5; e 197,8µ&#x3B5;). Estes resultados mostraram um aumento crescente da deformação média tanto nos intermediários como no poliuretano durante todas as etapas de confecção, com excessão da fundição em monobloco que atingiu altos valores, próximos da prótese finalizada. Desta forma, o procedimento de soldagem diminuiu os níveis de derformação, enquanto a aplicação da cobertura cerâmica, glaze, acabamento e polimento, aumentaram as deformações. / Passive fit and stability have become the most important prerequisites during the choice of materials and techniques to implant-supported prostheses. Therefore, the aim of this study was to verify the mean deformation in abutments and simulated bone tissue around dental implants, with the use of strain gages, during framework screw retention, analyzing seven fabrication stages of the fixed partial denture. For this research, an experimental model based on homogeneous polyurethane with two external hexagon implants parallel to each other, provided with 5 mm height multi-unit abutments. For each, abutment surface received three equidistant strain gauges and the experimental block received upper surface four strain gauges, positioned on the mesial, distal, buccal and lingual aspects. The tests were performed screwing the specimens on the abutment using an electronic torque with 10 Ncm maximum force. The readings were made in seven moments, with abutment and polyurethane mean deformation, respectively: 1) control group: cobalt-chromium prefabricated machined cylinder (36,4µ&#x3B5; e 87,5µ&#x3B5;), 2) framework fabricated by 1-piece method in nickel-cobalt-chromium alloy (340,3µ&#x3B5; e 131,4µ&#x3B5;), 3) framework sectioned with space for welding (43,2µ&#x3B5; e 66,6µ&#x3B5;), 4) weld space filled with chemically activated acrylic resin (Duralay) (74µ&#x3B5; e 86,8µ&#x3B5;), 5) framework sectioned and soldered (280µ&#x3B5; e 146,2µ&#x3B5;), 6) framework welded and ceramic application (288,7µ&#x3B5; e 151,1µ&#x3B5;) and 7) framework concluded applying glaze, finishing and polishing (359,9µ&#x3B5; e 197,8µ&#x3B5;). The results showed the mean strain increase in abutment and polyurethane over fabrication stages, except that framework fabricated by 1-piece method reached high values close to finished prostheses. Thus, the welding procedure decreased strain levels in simulated bone tissue around dental implants, while ceramic and glaze application, finishing and polishing procedures, increased strain.

MACEDO, A. A. M.Filmes de colágeno - polissacarídeo sulfatado como matrizes com propriedades antitrombogênicas: preparação e caracterização. 2006. 77f. Dissertação (Mestrado em Bioquímica)- Centro de Ciências, Universidade Federal do Ceará, 2006. / Submitted by JOANA BEZERRA (joanabib@yahoo.com.br) on 2013-01-07T19:14:17Z No. of bitstreams: 1 2006_dis_aammacedo.pdf: 1291383 bytes, checksum: 82d27442aec13166192157ba1d4a7a47 (MD5) / Approved for entry into archive by Érica Barros(ericabarros@ufc.br) on 2013-01-09T16:59:11Z (GMT) No. of bitstreams: 1 2006_dis_aammacedo.pdf: 1291383 bytes, checksum: 82d27442aec13166192157ba1d4a7a47 (MD5) / Made available in DSpace on 2013-01-09T16:59:11Z (GMT). No. of bitstreams: 1 2006_dis_aammacedo.pdf: 1291383 bytes, checksum: 82d27442aec13166192157ba1d4a7a47 (MD5) / This work reports the preparation and characterization of collagen-sulfated polysaccharide films and applications in coating of cardiovascular prostheses. The films were prepared by adding the sulfated polysaccharide solution on soluble collagen (0, 5, 10, 20 and 25 %). The blends of collagen-sulfated polysaccharide were casted in acrylic molds, and dried at low temperature. The physico-chemical properties of collagen-sulfated polysaccharide films, were studied using infrared spectroscopy that showed absorptions typical of collagen and sulphated polysaccharide; the thermal analysis (DSC) showed that the structural integrity of collagen was unmodified during the extraction process and the polysaccharide present in the samples did not decrease the thermal stability of the collagen, which was higher for the concentrations of 20 %. In the thermal analysis (DTG), the addition of the polysaccharide caused a small decrease of the stability of thermal degradation and when was adding 5 % of the sulfated polysaccharide to the collagen, a reduction in its stability occurs, however, in the concentrations of 10, 20 and 25 %, the stability of thermal degradation of the film increased. In the impedance spectroscopy, the results are associated possibly, to the interactions between the macromolecules. The blood compatibility was analyzed by platelet adhesion and the results showed that the films possess hemocompatibility and antitrombogenic properties. / Este trabalho aborda sobre o preparo e a caracterização de filmes de colágeno-polissacarídeo sulfatado e sua aplicação para revestimento de próteses vasculares. Os filmes foram obtidos a partir da mistura de solução de colágeno e polissacarídeo sulfatado, nas concentrações 0, 5, 10, 20 e 25 % do polissacarídeo. As soluções de colágeno-polissacarídeo sulfatado foram formatadas em moldes de acrílico em condições de baixa umidade e temperatura. A caracterização físico-química dos filmes de colágeno-polissacarídeo sulfatado foi estudada por espectrometria no infravermelho que mostraram bandas típicas do colágeno e bandas típicas do polissacarídeo sulfatado; a análise térmica por DSC mostra que a integridade estrutural do colágeno foi preservada no processo de extração e que a adição do polissacarídeo nas amostras não diminuiu a estabilidade térmica do colágeno tendo sido maior na concentração de 20 %. Na análise térmica por DTG, a adição do polissacarídeo causa um pequeno decréscimo da estabilidade de degradação térmica e ao adicionarmos 5 % do polissacarídeo sulfatado ao colágeno, ocorre uma redução em sua estabilidade, porém, a partir daí, nas concentrações 10, 20 e 25 %, a estabilidade de degradação térmica do filme vai aumentando. Na espectroscopia de impedância, os resultados estão associados, possivelmente, a interações entre as macromoléculas. A compatibilidade sangüínea foi analisada por adesão de plaquetas e os resultados mostraram que os filmes possuem hemocompatibilidade e propriedades antitrombogênicas.

Submitted by Claudinei Pracidelli (cpracide@ipen.br) on 2016-06-22T14:30:57Z No. of bitstreams: 0 / Made available in DSpace on 2016-06-22T14:30:57Z (GMT). No. of bitstreams: 0 / Tese (Doutorado em Tecnologia Nuclear) / IPEN/T / Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP