Made available in DSpace on 2016-06-02T19:32:06Z (GMT). No. of bitstreams: 1 5012.pdf: 1905383 bytes, checksum: a0998ff6fc2a6a32283393c52fcdd06f (MD5) Previous issue date: 2013-02-25 / Universidade Federal de Sao Carlos / Landscape fragmentation and habitats loss caused by human activities have led to a decline cougar populations (Puma concolor). Delimiting populations and establish rates of migration between them is essential to understanding its dynamics and therefore planning conservation measures. The present study propose to use molecular techniques with noninvasive samples to evaluate the status of a population of P. concolor inhabiting in a patch of 32km2 of São Paulo State. The population size was of 6 individuals and its density ranged between 4.6 and 1.8/100km2. In addition, this population was analyzed together with other individuals of P. concolor collected in the northeast region of the state. Bayesian clustering methods were used to identify genetic populations, and Bayesian multilocus genotyping method to estimate migration rates, in order to determinate possible source-sink dynamics. Finally, two subpopulations separated by the highway SP-310 were identified. The results showed that the subpopulation located on the east of the highway behaved as source population and the another one found on west as a sink. Future researches of P. concolor populations must aim to determinate the populations structures and thereby facilitate the establishment of effective action plans for the conservation of the species. / A contínua fragmentação e perda de habitats ocasionados pela ação humana têm levado ao declínio de populações de onça-parda (Puma concolor). Delimitar as populações, e estabelecer as taxas de migração entre elas é essencial para entender a dinâmica populacional e, consequentemente, planejar medidas conservacionistas. No estudo foram utilizadas técnicas moleculares com amostras não invasivas para avaliar uma população de P. concolor em um fragmento de cerrado de 32km2 no estado de São Paulo, o qual apresentou um tamanho populacional de 6 indivíduos e uma densidade que variava entre 4,6 e 1,8/100km2. Adicionalmente, esta população foi analisada em conjunto com outros indivíduos de P. concolor coletados na região nordeste do estado. O método de cluster Bayesiano foi utilizado para o estabelecimento de populações genéticas, e o método Bayesiano de genótipos multiloci para estimar as taxas de migração a fim de determinar uma possível dinâmica fontesumidouro. Finalmente, foram identificadas duas subpopulações, que tinham com barreira a rodovia SP-310. A partir dos resultados constatou-se que a subpopulação localizada ao leste da rodovia se comportava como população fonte e a subpopulação ao oeste como sumidouro. Futuras pesquisas acerca populacionais de P. concolor deverão visar a determinação da estrutura populacional e desse modo favorecer o estabelecimento de planos de ação eficientes para a conservação da espécie

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-08-26T23:22:11Z No. of bitstreams: 1 ValmarDaSilvaSeverianoSobrinho_DISSERT.pdf: 5455840 bytes, checksum: 21030bd15bb16fb2597187481d68f757 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-08-31T19:35:17Z (GMT) No. of bitstreams: 1 ValmarDaSilvaSeverianoSobrinho_DISSERT.pdf: 5455840 bytes, checksum: 21030bd15bb16fb2597187481d68f757 (MD5) / Made available in DSpace on 2016-08-31T19:35:17Z (GMT). No. of bitstreams: 1 ValmarDaSilvaSeverianoSobrinho_DISSERT.pdf: 5455840 bytes, checksum: 21030bd15bb16fb2597187481d68f757 (MD5) Previous issue date: 2016-05-12 / Filmes finos de TiO2 podem ser a resposta para grandes quest?es atuais sobre as melhores maneiras de obter energia, economizar energia e reduzir a polui??o. Tais filmes t?m sido aplicados com sucesso para produ??o de c?lulas solares; como camada em janelas inteligentes, janelas fotocr?micas e eletrocr?micas; al?m de possu?rem propriedades fotocatal?tica interessantes. Este trabalho explora a import?ncia tecnol?gica e cient?fica desse material realizando investiga??es a respeito dos filmes de TiO2, sua forma de deposi??o, t?cnicas de an?lise, detalhando formas atuais de an?lise ?ptica, buscando, de maneira inovadora, comparar tais t?cnicas, validar seu uso, e comparar seus resultados na busca de meios econ?micos da realiza??o de investiga??es a respeito de espessura, estrutura e avalia??o de propriedades fotocatal?ticas do material produzido. Nesse trabalho foram utilizadas, para cria??o de filmes finos, deposi??o f?sica pelo m?todo de magnetron sputterin. Para an?lise ?ptica e c?lculo de espessura e Band Gap dos filmes ser? apresentado o M?todo do Envelope que foi originalmente proposto por Manifacier (Manifacier, Gasiot e Fillard, 1976) sendo que mais tarde Swanepoel (1983) conseguiu melhorar ainda mais a precis?o desse m?todo para encontrar propriedades ?pticas dos filmes finos e sua espessura. Tamb?m ser? apresentado, a aplica??o de equa??es propostas nos trabalhos de Lindgren (Lindgren et al., 2003) e a Lei de Beer-Lambert para c?lculo do Coeficiente de Absor??o dos filmes, outro dado importante para mais tarde determinar o gap dos mesmos, que ser? encontrado pelo M?todo Tauc. Para filmes muito finos, com poucas ou nenhuma franja de interfer?ncia se faz necess?rio o estudo de algum M?todo Computacional para determina??o dos seus par?metros ?pticos e espessura. Para tanto utilizou-se o M?todo PUMA, um M?todo Computacional desenvolvido por pesquisadores da UNICAMP/USP. Os filmes depositados foram analisados por DRX, EDS, al?m de serem submetidos a an?lise ?ptica, MEV transversal buscando validar os m?todos ?pticos em termos das espessuras recuperadas, al?m de encontrado seus Band Gap e seus valores comparados com o esperado pela literatura confrontando tais resultados com a cristalinidade obtida para os filmes. / TiO2 thin films have been successfully used in solar cells, smart windows, photochromic and electrochromic windows and also as photocatalytic coatings. In this work, TiO2 thin films were deposited by magnetron sputtering on a glass substrate and their optical properties, thickness, microstructure and photocatalytic properties were evaluated. Film thicknesses and band gaps were determined by the Swanepoel method using the envelopes in the transmission spectrum and also the PUMA computational method. The films were analyzed by X-ray Diffraction (XRD), Energy Dispersive X-ray Spectroscopy (EDS), Scanning electron microscopy (SEM) and optical analysis. SEM measured thicknesses were compared to those obtained using the optical methods. The PUMA method proved advantageous for thickness determination of thin films, particularly when the interference fringes are not evident in the transmission spectrum. In addition, film thicknesses determined using the PUMA method were in better agreement with SEM measurements than those determined by the Swanepoel Method.

It's been almost 70 years since the end of the worst military conflict of all times, the World War II, during which millions of people died. It is a sad fact that it still causes casualties even today. The problem lies in thousands of different pieces of munitions located throughout Europe. The biggest problem is posed by unexploded aerial bombs that have in recent years took its toll on bomb disposal experts as well as other people. The problem of aerial bombs are mainly American and British origin, which bear long-term mechanical - chemical igniter. The design of these igniters does not allow their simple unscrewing from the body itself, and even the handling of these aerial bombs may cause its explosion and the threat to all persons located in its vicinity. Even in our country can be found these air bombs with this type of igniter. This thesis deals with the possibilities of such a disposal with regard to economic and technological possibilities of the Czech Police bomb disposal experts. Therefore, research was conducted with a focus on how best to dispose of aerial bombs. Bomb disposal experts were approached to give their professional opinions. A part of the research was the study of foreign literature dealing with this issue. The sad fact is that this problem is not satisfactorily resolved anywhere in Europe. Abroad, various methods were used as appropriate, such as using high pressure water cutting or blasting the use of special keys. As demonstrated by accident in 2010 when in German Göttingen three bomb disposal experts died in liquidation of aerial bombs using high-pressure cutting, this method and similar have proven to be inappropriate. The preparation of these special methods takes an excessively long time, and in many cases the bomb requires manipulation. Bomb disposal experts of the Czech Republic along with other experts are developing and improving the explosive separation method using a special explosive (ammunition). The most appropriate seems the use of long flexible bomb explosive: RAZOR made by a Czech company - VÚPCH Pardubice - Semtín. The explosive is attached to the body of the bomb either on its perimeter or in the axis of the bomb and this leads to the separation of the lighter or pull out the body of the lighter without explosion of the body of the bomb. Practical experience shows that the use of this special method can reduce time to prepare bomb disposal and the handling of the bomb is minimal or none. Unfortunately, there is always a risk that there will be accidental explosion of aerial bombs and therefore is imperative to comply with all safety measures to the maximum extent possible.

Made available in DSpace on 2014-06-11T19:25:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-12-20Bitstream added on 2014-06-13T20:53:45Z : No. of bitstreams: 1 000739583.pdf: 1703307 bytes, checksum: 8c02eba2666e577572e313859a482bfa (MD5) / Ocular tumors play an increasing concern in veterinary ophthalmology. Corneal squamous cell carcinoma (SCC) is unfrequent in dogs, and by this way it has little studies, and the investigations of carcinogenesis mechanisms are rare. The aim of this work was to identify the p53, p63 and PUMA proteins expression in the spontaneous dog corneal SCC. For this work, were used five cases of corneal SCC and one case of actinic keratitis and their possible contributions to prognosis and therapy. The immunohistochemical analysis could evaluated the number of stained cells by field in optic microscopy using two classifications methods: intensity and immunofrequency. Also, we could evaluated histological grade of tumor related to malignancy in corneal SCC cells by using the mitotic index as a pattern. All samples showed immunolabelling to those proteins studied, although with diversity in intensity and frequency. The authors couldn´t observe relationship between the biggest mitotic index, and, by this way, most malignancy, with the expressions of all analysed proteins. These results could support the conclusions that p53, p63 and PUMA proteins immunoexpression are present in canine corneal SCC and could give help to their carcinogenesis, but they don´t give a prognostic indicator of these tumors

Made available in DSpace on 2015-06-17T19:34:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-12-18. Added 1 bitstream(s) on 2015-06-18T12:49:15Z : No. of bitstreams: 1 000831264_20160218.pdf: 59515 bytes, checksum: dd091ed719d525e0c7c1b769d8895532 (MD5) Bitstreams deleted on 2016-02-18T10:00:24Z: 000831264_20160218.pdf,. Added 1 bitstream(s) on 2016-02-18T10:01:05Z : No. of bitstreams: 1 000831264.pdf: 788858 bytes, checksum: eef44c8d5a932cacaf91b3f082ee4975 (MD5) / A onça parda (Puma concolor) possui significativa expressão no controle de outros vertebrados. Ela está no topo da cadeia alimentar, o que demonstra a sua grande importância em seu habitat natural. A espécie apresenta-se na Lista nacional oficial de espécies da fauna ameaçadas de extinção - peixes e invertebrados aquáticos, do Ministério do Meio Ambiente brasileiro, publicada na Instrução Normativa n. 3, de 27 de maio de 2003, e atualizada pela Portaria n. 444, de 17 de dezembro de 2014. A análise ultrassonográfica abdominal em modo B, Doppler colorido e espectral, associada à avaliação clínico-laboratorial desses animais fornecerá valores de referências que auxiliarão na investigação de doenças, uma vez que, não existem relatos pretéritos no que tange a investigação sonográfica desses felinos. Foram realizados exames ultrassonográficos e laboratoriais em quatorze animais, sendo onze machos e três fêmeas, livres de Leishmania chagasi e provenientes do Centro de Reabilitação de Animais Silvestres, Campo Grande, MS, sendo calculadas as médias dos valores encontrados para título de análises. O fígado apresentou-se hipoecogênico. A parede da vesícula biliar hiperecogênica, medindo 0,21 cm. Os diâmetros da artéria aorta, veia cava caudal e veia portal foram respectivamente 1,42 cm, 1,43 cm, 0,84 cm e a fluxometria portal foi de 32,30 cm/s. O baço é hiperecogênico, medindo 3,25 cm em corte transversal. A velocidade da artéria esplênica foi 47,13 cm/s. A parede do estômago e intestino mediram 0,26 cm e 0,20 cm respectivamente. O comprimento da adrenal esquerda foi 3,52 cm e a direita 3,23 cm. A espessura da parede da bexiga foi de 0,22 cm, o comprimento renal direito 6,22 cm e o esquerdo 5,96 cm. O índice de resistividade da artéria renal direita foi de 0,51 e esquerda de 0,50. A ultrassonografia mostrou-se eficiente na avaliação abdominal de onças pardas e é promissora a utilização desta ... / The cougar (Puma concolor) has significant expression in the control of other vertebrates. She is at the top of the food chain, which shows its importance in their natural habitat. The species present in the official national list of animal species threatened with extinction - fish and aquatic invertebrates, the Ministry of the Brazilian Environment, published in the Normative Ruling. 3 of 27 May 2003, and updated by Ordinance N. 444 of 17 December 2014. The abdominal ultrasound examination in B, color and spectral Doppler, associated with clinical and laboratory evaluation of these animals provide values of references that will assist in the investigation of diseases, since there are no reports in the past tense respect the sonographic investigation of these cats. Ultrasound and laboratory tests were performed in fourteen animals, which eleven males and three females, free of Leishmania chagasi and from the Rehabilitation Center of Wild Animals, Campo Grande, MS, and computed the average values found for under analysis. The liver was presented hypoechoic. The wall of the gallbladder hyperechogenic, measuring 0.21 cm. The diameter of the aorta, vena cava and portal vein were respectively 1.42 cm, 1.43 cm, 0.84 cm and flowmetry gate was 32.30 cm/s. The spleen is hyperechogenic, measuring 3.25 cm in cross section. The speed of the splenic artery was 47.13 cm/s. The wall of the stomach and intestine measured 0.26 cm and 0.20 cm, respectively. The length of the left adrenal was 3.52 cm and 3.23 cm right. The thickness of the bladder wall was 0.22 cm, the right renal length 6.22 cm and 5.96 cm left. The right renal artery resistance index was 0.51 and left 0.50. Ultrasonography was efficient in the abdominal assessment brown ounces and is promising to use this tool in the clinical routine of wild animal medicine

Orientadores: Eleonore Zulnara Freire Setz, José Eduardo Mantovani / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-20T00:59:02Z (GMT). No. of bitstreams: 1 Penteado_MarcelJoseFranco_D.pdf: 10454343 bytes, checksum: d8c60e67c27ebfc4bdb666229a8d4b1e (MD5) Previous issue date: 2012 / Resumo: O puma (Puma concolor) e a jaguatirica (Leopardus pardalis) são dois dos felídeos com maior distribuição geográfica no continente americano, ocorrendo em biomas que vão desde regiões muito secas até florestas tropicais. Assim como todos os predadores de porte médio e grande, necessitam de grandes áreas para sobreviver e são particularmente vulneráveis a extinções locais em ambientes fragmentados, onde seu desaparecimento pode levar a altas densidades de pequenos mamíferos predadores, ou mesopredadores, e ao empobrecimento dos níveis tróficos mais baixos. Além da perda de habitat, estas espécies sofrem com a falta de informações sobre sua ecologia, incluindo área de vida, uso de habitat e padrões de deslocamento e dispersão, essenciais para elaborar planos de manejo e estratégias de conservação eficientes. O presente trabalho analisou algumas destas informações em duas áreas com níveis significativos de impactos antrópicos (municípios de Paulínia, Cosmópolis, Americana e Atibaia), através do monitoramento de um puma por radiotelemetria GPS e três jaguatiricas por radiotelemetria VHF. Descrevemos a área de vida do puma com 11.400ha. Identificamos seleção para ambientes com cobertura vegetal arbórea e uso de matas ciliares como rotas de deslocamento. Também verificamos que seu nível de atividade decresce conforme a noite avança. Foi possível determinar locais prefereciais de predação através da análise de clusters, com indicações de que o puma monitorado identificou os melhores locais para predação de animais de maior porte. Também estimamos a área de vida para três jaguatiricas, variando entre 153 e 680ha, dentro do intervalo descrito para áreas de floresta tropical, mas muito inferior à descrita para outras áreas do continente americano, como o Pantanal e Iguaçú. Também verificamos preferência por ambientes com cobertura vegetal densa. Considerando os resultados obtidos, estratégias eficientes para a preservação destas espécies dependem da manutenção de áreas de mata nativa, interligadas por corredores de deslocamento, que permitam o trânsito destes animais entre eles e o contato e fluxo gênico entre populações distintas / Abstract: The cougar (Puma concolor) and the ocelot (Leopardus pardalis) are broadly distributed in the Americas, and occurs in biomes ranging from dry areas to tropical forests. As all medium to large sized predators, they need large landscapes to survive, and may be particularly vulnerable to local extinction in fragmented landscapes, where their disappearance can lead to higher densities of small mammalian predators, or mesopredators, and the impoverishment of lower trophic levels. Besides the loss of habitat, these species suffer from a lack of information about its ecology, including home range, habitat use and patterns of movement and dispersion, essential to develop effective management action plans and conservation strategies. The present study examined some of this information in two areas with significant levels of human impacts (municipalities in Paulínia, Cosmópolis, Americana and Atibaia), by monitoring a cougar by GPS radio telemetry and three ocelots by VHF radio telemetry. We describe the cougar's home range with 11.400ha (100% MCP). We found string selection for habitats with dense forest cover, and use of riparian areas as travel routes. We also found that their activity level decreases as the night progresses. We determinate preferecial predation sites by cluster analysis, with indications that the tracked cougar identified the best places to predation of larger animals. We also estimate the home range for three ocelots, ranging between 153 and 680ha (100% MCP) within the range reported for tropical forest, but much lower than that reported for other areas of the Americas, including brazilian Pantanal and Iguaçú. We also observed a preference for environments with dense vegetation. Considering these results, efficient strategies for the preservation of these species depend on maintaining areas of native forest, connected by movement corridors, allowing the transit of these animals between them and the contact and gene flow between different populations / Doutorado / Ecologia / Doutor em Ecologia

Seventeen cougars (Felis concolor) utilizing the Junction Wildlife Management Area (W.M.A.) in central British Columbia were fitted with radio collars. All collared cougars within the area were relocated using ground-based and aerial radio telemetry. Relocations were made daily during intensive field work (December-August), and a minimum of four per week the remainder of the year. General site reconnaissance and direct sampling work from 1986 to 1988 revealed 132 prey species mortalities of which 50 were confirmed as recent cougar kills. Although bighorn ewes and lambs (Ovis canadensis californiana) were not important prey items for the cougars, bighorn rams comprised 77.6% of the total mortality sample and 46.5% of the confirmed cougar kills. Cougars selected rams in greater proportion than would be expected based on the availability of rams in the prey population. Poor post-rut body condition and restricted rear and peripheral vision were factors that increased the rams' vulnerability to cougar predation. Cougar predation rates on bighorn sheep and mule deer (Odocoileus hemionus hemionus) were determined for two females with kittens. Kill rates varied from 0.7 - 3.0 ungulates/week. Interactions between cougars and coyotes (Canis latrans) at kill sites influenced the cougars' utilization of kills and predation rates. In 200 km² of the 425 km² study area, 130 coyotes were removed over a two-year period. The predation rate of a cougar with three kittens within the coyote removal area averaged 1.1 kills/week while that of a female with two similarly-aged kittens in the non-removal area averaged 2.6 kills/week. Moreover, observations of cougars abandoning kills following harassment by coyotes, suggested that cougar/coyote interactions were an important part of the system. Poor lamb recruitment and a decline in the number of mature rams in the Junction herd are a concern for the Ministry of Environment (MOE) Wildlife Branch. I make two recommendations that address these concerns: 1. Maintain the resident cougar population without removing cougars. Cougars were not important predators of the lamb segment, nor were cougars keying on the older, larger rams. Moreover, removal of the resident cougar population will disrupt the intraspecific and territorial dynamics of the cougar population resulting in an influx of transient cougars. This, in turn, will lead to the Junction system stabilizing at cougar numbers equal to or possibly greater than pre-removal levels. 2. Initiate an alternating, two-year on, two-year off, February-April coyote removal program until Iamb recruitment remains above 20 lambs/100 ewes throughout a four-year cycle. This program is preferable to cougar removal in that coyote removal can be implemented more effectively on a temporally and spatially scale. Coyote removal will result in an increase in lamb recruitment to the bighorn population, including the ram component. Moreover, fewer rams from this increased population will be killed because of lower cougar predation rates that also will result from the decrease in coyote scavenging/displacement pressures. / Science, Faculty of / Zoology, Department of / Graduate

An increasing body of evidence points to a key role of endoplasmic reticulum (ER) stress in chronic and acute neurodegenerative diseases. Indeed, markers of ER stress are common features of neurons destined to die in these conditions. In the present study we demonstrate that PUMA, a BH3-only member of the Bcl-2 family is essential for ER stress-induced cell death. PUMA is known to be a key transcriptional target of p53, however we have found that ER stress triggers PUMA induction and cell death through a p53-independent mechanism involving instead the ER stress inducible transcription factor ATF4. Specifically, we demonstrate that ectopic expression of ATF4 sensitizes neurons to ER stress induced apoptosis, and that ATF4-deficient neurons exhibit markedly reduced levels of PUMA expression and cell death. However, chromatin immunoprecipitation experiments suggest that ATF4 does not directly regulate the PUMA promoter. Rather, we found that ATF4 induces expression of the transcription factor CHOP, and that CHOP in turn directly activates PUMA induction. Specifically, we demonstrate that CHOP binds to the PUMA promoter during ER stress and that CHOP knockdown attenuates PUMA induction and neuronal apoptosis. In summary, we have identified a key signaling pathway in ER stress induced neuronal death involving ATF4-CHOP mediated transactivation of the pro-apoptotic Bcl-2 family member PUMA. Protein aggregates and markers of ER stress response have also been observed in dying neurons in several animal models of cerebral ischemia. Therefore, to decipher the significance of the ER stress apoptotic response, we investigate the role of ATF4-CHOP signaling pathway in ischemic neuronal injury. Ischemic stroke results from a transient or permanent reduction in cerebral blood flow in the brain. In spite of much research in trying to develop therapeutic strategies, most clinical trials have failed. These failures demonstrate that effective treatments require a more complete understanding of molecular signals that lead to neuronal death. However, stroke is a complex scenario since distinct mechanisms may involve in rapid and/or delayed neuronal death. The signaling pathways regulating these mechanisms however are not fully defined. Previous studies had suggested that ER stress playing a pivotal role in post-ischemic neuronal death. Yet, the relevance of ER stress signals was not fully known in ischemic neuronal injury. Accordingly, this thesis research attempts to explore the functional role of ER stress -inducible pathway, ATF4-CHOP axis, in different models of neuronal death (delayed and excitotoxic cell death) evoked by ischemia. The data indicates that ATF4 is essential in delayed type of death in vitro. In focal ischemia model (tMCAO) ATF4 also plays a role as a mediator of death signal in vivo. However, CHOP function looks more complex, and our data did not support the role of CHOP in ischemic neuronal death.

The objective of this research was to study the impacts of large USSE facilities on wildlife movement corridors and habitat connectivity in Florida to identify important habitat patches and movement corridors significant for potential Florida panther (Puma concolor coryi) dispersal, conservation, and recovery. Large carnivores (such as Florida panther) have often been examined as conservation umbrellas for large-scale connectivity planning due to their extensive spatial requirements (Beier et al., 2006, Thorne et al., 2006). In addition, Florida panther is relatively well-studied species that have been listed as endangered under the US Endangered Species Act for over 40 years, due to habitat fragmentation and reduction. Habitat suitability was compared before and after the installation of the USSE facilities using RandomForest model developed by Frakes and Knight (in prep). The connectivity study focused on comparing current density and effective resistance (resistance distance) before and after the installations of USSE facilities and was conducted using Circuitscape 4.0. The impacts on habitat connectivity were the most substantial in the areas where the USSE facilities were installed on or around wildlife corridors, near focal areas, and in large clusters. The study has identified important linkages and new pathways that animals may try to take in order to bypass the facilities. The study has also shown that installation of USSE facilities in areas not suitable as habitat for Florida panthers, but still permeable or semi-permeable (e.g., agricultural lands), can substantially impact connectivity between focal areas. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2020. / FAU Electronic Theses and Dissertations Collection

A widely used dynamical core, PUMA (Portable University Model of the Atmosphere), is validated using the test case specifications introduced by Polvani et al. (2004). Dynamical cores are essential in every presently used AGCM (Atmospheric General Circulation Model), and deal with the dry, adiabatic primitive equations. The validation process is important in order to establish that the dynamical core is free from bugs, and thereby confirm the validity AGCMs. The test case proposed by Polvani et al. (2004) consists of a 12 day time-integrated slightly perturbed, baroclinically unstable, midlatitude jet, and is, together with derivations of the model equations, completely described in this M. Sc. thesis. The initial condition for the test case is implemented in PUMA, to a modification in order to carry out the same test case as in Polvani et al. (2004). The temperature and vorticity fields are presented and compared. The numerically converged solutions from PUMA are in strong accordance with the solutions from Polvani et al. (2004), despite different numerical schemes to solve the equations. This supports the validity and reliability for future studies with PUMA. / En torr, adiabatisk primitiv ekvationsmodell, PUMA (Portable University Model of the Atmosphere), valideras genom att jämföra dess lösningar med resultaten som presenterades av Polvani et al. (2004). Primitiva ekvationsmodeller är en viktig del i dagens allmänna cirkulationsmodeller. Valideringsprocessen är viktig för att fastslå att de primitiva ekvationsmodellerna fungerar utan buggar, och därigenom bekräfta att en viktig del av de allmänna cirkulationsmodellerna fungerar tillfredsställande. Initialtillståndet som presenterades av Polvani et al. (2004) består av en 12 dagars tids-integrerad, något perturberad, baroklint instabil polarjet. Initialtillståndet är, tillsammans med härledningarna av modellekvationerna, fullständigt beskriven i detta examensarbete. Initialtillståndet implementeras i PUMA, vilken har modifierats för att konstruera samma testspecifikationer som i Polvani et al. (2004). Temperatur- och virvlingsfälten presenteras och jämförs. De numeriskt konvergerade lösningarna från PUMA stämmer väl överrens med lösningarna från Polvani et al. (2004), trots att olika numeriska scheman använts för att lösa ekvationerna. Detta stödjer validiteten hos PUMA, vilket ökar tillförlitligheten i framtida studier med modellen.