Afin de faire face aux fortes températures rencontrées par les composantsen aval de la chambre de combustion, des prélèvements d’air plus frais sont réalisésau niveau du compresseur. Cet air alimente les cavités en pied de turbine et refroidiles disques rotor permettant d’assurer le bon fonctionnement de la turbine.Ce manuscrit présente une étude numérique de l’effet de ces écoulements de cavitéau pied de la turbine sur ses performances aérodynamiques. Les phénomènesd’interaction entre l’air de cavité en pied de turbine et l’air de veine principal est unphénomène encore difficilement compris. L’étude de ces phénomènes est réalisée autravers de différentes approches numériques (RANS, LES et LES-LBM) appliquéesà deux configurations pour lesquelles des résultats expérimentaux s ont disponibles.Une première configuration en grille d’aube linéaire en amont de laquelle différentesgéométries d’entrefer (interface entre plateforme rotor et stator) et débits de cavitépouvaient être variés. Une seconde configuration annulaire composée de deux étagesde turbine comprenant les cavités en pied et plus proche d’une configuration industrielle.Les pertes additionnelles associées à l’écoulement de cavité sont mesurées etétudiées à l’aide d’une méthode basée sur l’exergie (bilans d’énergie dans l’objectifde générer du travail). / In order to deal with high temperatures faced by the components downstreamof the combustion chamber, some relatively cold air is bled at the compressor.This air feeds the cavities under the turbine main annulus and cool down the rotordisks ensuring a proper and safe operation of the turbine. This thesis manuscriptintroduces a numerical study of the effect of the cavity flow close to the turbine hubon its aerodynamic performance. The interaction phenomena between the cav-ity andmain annulus flow are not currently fully understood. The study of these phenomenais performed based on different numerical approaches (RANS, LES and LES-LBM)applied to two configurations for which experimental results are avail-able. A linearcascade configuration with an upstream cavity and various rim seal geometries(interface between rotor and stator platform) and cavity flow rate avail-able. Arotating configuration that is a two stage turbine including cavities close to realisticindustrial configurations. Additional losses incurred by the cavity flow are measuredand studied using a method based on exergy (energy balance in the purpose togenerate work).

L’analyse du mouvement, ou l’analyse d’une séquence d’images, est l’extension naturelle de l’analyse d’images à l’analyse de séries temporelles d’images. De nombreuses méthodes d’analyse de mouvement ont été développées dans le contexte de la vision par ordinateur, incluant le suivi de caractéristiques, le flot optique, l’analyse de points-clef, le recalage d’image, etc. Dans ce manuscrit, nous proposons une boite a outils de techniques d’analyse de mouvement adaptées à l’analyse de séquences biomédicales. Nous avons en particulier travaillé sur les cellules ciliées qui sont couvertes de cils qui battent. Elles sont présentes chez l’homme dans les zones nécessitant des mouvements de fluide. Dans les poumons et les voies respiratoires supérieures, les cils sont responsables de l’épuration muco-ciliaire, qui permet d’évacuer des poumons la poussière et autres impuretés inhalées. Les altérations de l’épuration mucociliaire peuvent être liées à des maladies touchant les cils, pouvant être génétiques ou acquises et peuvent être handicapantes. Ces maladies peuvent être caractérisées par l’analyse du mouvement des cils sous un microscope avec une résolution temporelle importante. Nous avons développé plusieurs outils et techniques pour réaliser ces analyses de manière automatiques et avec une haute précision, à la fois sur des biopsies et in-vivo. Nous avons aussi illustré nos techniques dans le contexte d’éco-toxicité en analysant le rythme cardiaque d’embryons de poissons / Motion analysis, or the analysis of image sequences, is a natural extension of image analysis to time series of images. Many methods for motion analysis have been developed in the context of computer vision, including feature tracking, optical flow, keypoint analysis, image registration, and so on. In this work, we propose a toolbox of motion analysis techniques suitable for biomedical image sequence analysis. We particularly study ciliated cells. These cells are covered with beating cilia. They are present in humans in areas where fluid motion is necessary. In the lungs and the upper respiratory tract, Cilia perform the clearance task, which means cleaning the lungs of dust and other airborne contaminants. Ciliated cells are subject to genetic or acquired diseases that can compromise clearance, and in turn cause problems in their hosts. These diseases can be characterized by studying the motion of cilia under a microscope and at high temporal resolution. We propose a number of novel tools and techniques to perform such analyses automatically and with high precision, both ex-vivo on biopsies, and in-vivo. We also illustrate our techniques in the context of eco-toxicity by analysing the beating pattern of the heart of fish embryo

The ultimate objective of this research was to develop a low cost, reliable system that would lead to early detection of lung cancer. Tests involved the quantitation of gaseous metabolic emissions from immortalized lung cancer cell lines in order to correlate the chemical markers to be of cancerous origin. The specific aims of the project were the study of gas emissions in selected cancer cell lines and identification of volatile organic compounds (VOCs) in them. Disadvantages of earlier studies were that the measurements were not real time or state specific so that molecular identification was often inconclusive. Furthermore the methods of study used in the past were not quantitative, which limited their practicality for medical applications. We felt the need to prove or disprove these earlier results using a new technique. The method we proposed is different and unique when compared to previous methods because cell lines have not been studied extensively for cancer markers. We have studied cancer cell lines which are adherent, immortalized cultures originating from primary tumors obtained from patients with no prior treatment for lung cancer. We have used an alternative method for the spectrometric analysis and quantitation of the selected chemical markers. The pre-concentration method involved a Purge and Trap unit with a thermal desorber where the vapor concentration was enhanced. The concentrated head space gases were analyzed using a Gas Chromatograph ?? Mass Spectrometer setup. This setup eliminated the bulky apparatus used in earlier studies. It is simpler in design and more comprehensive so that external factors such as patient??s diet, habitat and lifestyle do not contribute to our study of recognition of cancer markers. Based on the results obtained in the above experiments, a more comprehensive, inexpensive study of lung cancer related markers could be made. The first section, after giving an introduction to lung cancer, goes on to explain the background work done by other researchers on cancer. The third section gives a detailed explanation of the experimental setup. This is followed by all the tests conducted with corresponding results. The final section deals with the conclusions drawn from all experiments.

This work concerns the validation of two 1D Turbine Design Tools, AXIAL by Concepts NREC and TML by GKN Aerospace, and is purely computational. By using the KTH Test Turbine as a reference frame, these two software programs were set up to simulate its performance, and the results consequently validated against existing experimental data from the turbine. The main objective of this work is to investigate the performance prediction abilities of the 1D Design Tools for a variety of turbine parameters such as efficiency, mass flow, power output and degree of reaction, and study the accuracy of these predictions under given boundary conditions, namely turbine stage inlet pressure, temperature and pressure ratio. The main focus of the simulation was to evaluate the impact of the choice of loss model in the 1D Software Tools for estimation of losses. Thus, in order to gain a better understanding of differences and similarities among the scope of available loss models, as well as deviation models, a literature study was performed. Additionally, in order to extend the knowledge of the detailed performance prediction characteristics of these software tools in regard to the loss model implemented, the individual loss coefficients (profile, secondary, trailing edge, tip clearance and incidence) were studied and analysed. The impact of chosen pressure ratio on the 1D simulation accuracy was also investigated. The software tool used and the loss model selected were both found to be of great significance to the accuracy of the simulated performance. The pressure ratio (PR) used for simulation also proved to be of great significance, with simulations performed at an elevated PR providing considerably more accurate results than at the design PR, suggesting that the majority of loss models are more accurate when estimating with higher PR. The KTH Test Turbine stage validated in this work featured a number of special geometrical features of inconvenient nature for 1D simulations. In order to account for this, a number of correction coefficients were developed and implemented and their individual effect on the simulated performance studied. Another special feature of the turbine stage studied was the lean angle of the stator, which impact on the 1D simulations was also investigated. Additionally, a number of different user selectable parameters in AXIAL and their impact on the simulations were investigated. The geometry correction coefficients and stator lean angle were found to be of negligible impact to the overall estimated performance, while the user selectable parameters in AXIAL proved to be of relatively big influence on the simulated results. Lastly, using the TML software tool, the concept of stator-rotor disc cavity flow known as 'purge flow' was simulated and validated against reference data. Purge flow serves to inhibit the inflow of hot air from the main annulus to the inner hub and simultaneously cool the rotor blades. The TML software was found to overestimate the losses associated with the use of purge flow, although providing relatively coherent trends for parameters such as efficiency, mass flow and power, suggesting that a correction coefficient applied to the overall losses from purge flow could potentially provide better overall accuracy in the simulations. / Swedish TURBOPOWER Research Program

The dairy food industry is continuously striving towards products with higher quality and longer shelf-life available to the customer at low prices. Arla Foods in Linköping, Sweden, is currently investigating the possibilities of changing the material in yogurt packaging containers by replacing the currently used carton with a different and cheaper alternative. A successful switch will give the company an economical advantage without affecting the sensory attributes (smell, taste, sight, and consistency), aroma profile or other important trademarks of the yogurt. This study is designed to examine and compare yogurt that has been stored in different packaging cartons, one coated with a single-layered low-density polyethylene (LDPE) and one coated with a currently used multi-layered ethylene-vinyl alcohol (EVOH). The study was based on the analysis and measurement of sensory attributes performed by experts, physical properties in laboratory and chemical composition in GC-FID/MS together with a discriminative test where a group of people would identify any difference between the yogurts. Together, these analyses would provide an explanation about any differences between the packaging materials by connecting physical, chemical and/or sensory characteristics. The collected results would give a better and more comprehensive picture than each analysis would do separately. The results from the study show that there is a difference between yogurts stored in LDPE-based containers and yogurts stored in EVOH-based containers and that the product was chemically affected, mainly by the level of oxygen in contact with the food. The overall assessment is that the largest difference was discovered in the taste.

Thesis (Masters) -- The College of Saint Elizabeth, 2010. / Typescript. Available at The College of Saint Elizabeth - Office of Graduate Programs. "March 2010"

Cette thèse porte un regard évolutif sur les invasions biologiques qui sont une menace écologique, économique et sanitaire grandissante.Qu'ils en soient la cause ou la conséquence, les invasions sont en effet le théâtre de changements évolutifs rapides et importants.Ces changements peuvent être liés à une différence de pression de sélection entre l'aire d'origine et l'aire envahie, mais peuvent aussi être initiés par des évènements démo-génétiques comme un goulot d'étranglement démographique ou l'admixture (hybridation intra-spécifique) entre populations génétiquement différentes.Les changements évolutifs concernent aussi bien des traits d'histoire de vie liés à la reproduction que le fardeau génétique et la dépression de consanguinité.La forte dérive génétique qui peut se produire au cours d'un goulot d'étranglement peut conduire à la purge ou la fixation rapide d'allèles délétères responsables du fardeau génétique.Les trais de reproduction peuvent eux aussi influencer directement l'issue d'une invasion, au travers du taux d'accroissement démographique de la population.Ils peuvent aussi avoir un effet indirect sur le succès d'introduction en modifiant l'intensité des effets des évènements démo-génétiques.Au cours de cette thèse, j'ai étudié l'évolution du fardeau génétique et de traits de reproduction au cours d'une invasion biologique grâce à (i) une série d'expériences sur l'espèce envahissante modèle Harmonia axyridis (la coccinelle asiatique) et à (ii) un modèle théorique de dynamique de la fréquence d'un allèle délétère dans une population subissant un goulot d'étranglement.Mes résultats montrent que les populations envahissantes ont des traits de reproduction plus performants que celles de l'aire native.En particulier, les femelles envahissantes présentent une fécondité plus élevée et fécondent leurs œufs avec le sperme d'un plus grand nombre de mâles.Il n'existe pas, chez cette espèce, de mécanisme évident d'évitement de la consanguinité, bien que les populations de l'aire native souffrent de dépression de consanguinité contrairement à celles de l'aire envahie.La dépression de consanguinité peut évoluer très vite chez cette espèce en cas de fort goulot d'étranglement.Dans les conditions expérimentales, les allèles délétères étaient parfois purgés, mais ont souvent été fixés dans la population au cours d'un goulot.Enfin, le modèle théorique a montré que les probabilités de perte ou de fixation d'un allèle délétère récessif peuvent toutes deux augmenter rapidement par dérive au cours d'un goulot d'étranglement d'intensité modérée.Ces résultats soulignent l'importance du hasard sur le succès d'une invasion biologique.Puisque la probabilité d'émergence de combinaisons génomiques favorables à l'invasion augmente avec le nombre d'introduction, limiter le nombre d'évènements d'introduction peut s'avérer déterminant pour prévenir de futures invasions biologiques. / This thesis provides some evolutionary insights on biological invasions, which represent a growing threat on ecology, economy and public health.Biological invasions are a place of rapid and important evolutionary changes that can be the cause or the consequence of invasion success.These changes can be caused by differences in selective pressures between the native and the invaded area.They can also be due to demo-genetic events such as demographic bottlenecks and admixture (emph{i.e.} intra-specific hybridisation) between genetically distinct populations.Evolutionary changes affect life-history traits (including reproductive traits) as well as the genetic load and inbreeding depression.Genetic drift can be strong during a bottleneck and can induce the rapid purging or fixation of deleterious alleles responsible for the genetic load.Reproductive traits can also influence the fate of an invasion by changing the demographic growth rate.Moreover, they can have an indirect impact on the success of introduction by altering the intensity of demo-genetic events.Throughout this thesis, I studied the evolution of the genetic load and of reproductive traits by the mean of (i) laboratory experiments using the invasive Harlequin ladybird Harmonia axyridis as a model species and (ii) through the study of a theoretical model of the dynamics of allele frequencies during a bottleneck.My results show that invasive populations have better reproductive traits than those from the native area.Particularily, invasive females display an increased fecundity and fertilize their eggs with the sperm of a higher number of males.In this species, there is no obvious mechanism of inbreeding avoidance, even though native populations suffer to inbreeding depression contrarily to invasive ones.Inbreeding depression can evolve quickly in this species if the population goes through a severe bottleneck.In my experiments, deleterious alleles were often fixed in the population during the bottleneck, but were sometimes purged.Finally, the theoretical model studied described show that the probability of complete loss or fixation of a deleterious recessive allele can both increase by drift during a bottleneck.These results highlight the importance of chance on the success of a biological invasion.Because the probability of emergence of genomic combinations that can favour an invasion increases with the number of introduction events, limiting the number of introduction events might be an efficient way to prevent or minimise upcoming biological invasions.

Целью работы является изучение влияния донной продувки в промежуточном ковше на содержание неметаллических включений в транспортном металле и пути повышения качества данных марок стали. В качестве решения проблемы рассмотрена гипотеза о зависимости содержания неметаллических включений в стали от состава шлака на установке «печь-ковш»; создана физическая модель МНЛЗ; предложена схема расположения аргонных блоков в промежуточном ковше, что позволит создать оптимальные потоки металла для флотации неметаллических включений. / The aim of the work is to study the effect of bottom blowing in the intermediate bucket on the content of non-metallic inclusions in the transport metal and ways to improve the quality of these steel grades. As a solution to the problem, the hypothesis of the dependence of the content of non-metallic inclusions in steel on the composition of the slag at the furnace-ladle installation is considered; a physical model of the CCM is created; a scheme for the arrangement of argon blocks in the intermediate ladle is proposed, which will create optimal metal flows for flotation of non-metallic inclusions.

No description available.

The study presented herein describes and analyzes a detailed experimental investigation of the effects of density ratio on endwall thermal performance at varying blowing rates for a typical nozzle guide vane platform purge jet cooling scheme. An axisymmetric converging endwall with an upstream doublet staggered cylindrical hole purge jet cooling scheme was employed. Nominal exit flow conditions were engine representative and as follows: {rm Ma}_{Exit} = 0.85, {rm Re}_{Exit,C_{ax}} = 1.5 times {10}^6, and large-scale freestream Tu = 16%. Two blowing ratios were investigated corresponding to the upper and lower engine extrema. Each blowing ratio was investigated amid two density ratios; one representing typical experimental neglect of density ratio, at DR = 1.2, and another engine representative density ratio achieved by mixing foreign gases, DR = 1.95. All tests were conducted on a linear cascade in the Virginia Tech Transonic Blowdown Wind Tunnel using IR thermography and transient data reduction techniques. Oil paint flow visualization techniques were used to gather quantitative information regarding the alteration of endwall flow physics due two different blowing rates of high-density coolant. High resolution endwall adiabatic film cooling effectiveness, Nusselt number, and Net Heat Flux Reduction contour plots were used to analyze the thermal effects. The effect of density is dependent on the coolant blowing rate and varies greatly from the high to low blowing condition. At the low blowing condition better near-hole film cooling performance and heat transfer reduction is facilitated with increasing density. However, high density coolant at low blowing rates isn't adequately equipped to penetrate and suppress secondary flows, leaving the SS and PS largely exposed to high velocity and temperature mainstream gases. Conversely, it is observed that density ratio only marginally affects the high blowing condition, as the momentum effects become increasingly dominant. Overall it is concluded density ratio has a first order impact on the secondary flow alterations and subsequent heat transfer distributions that occur as a result of coolant injection and should be accounted for in purge jet cooling scheme design and analysis. Additionally, the effect of increasing high density coolant blowing rate was analyzed. Oil paint flow visualization indicated that significant secondary flow suppression occurs as a result of increasing the blowing rate of high-density coolant. Endwall adiabatic film cooling effectiveness, Nusselt number, and NHFR comparisons confirm this. Low blowing rate coolant has a more favorable thermal impact in the upstream region of the passage, especially near injection. The low momentum of the coolant is eventually dominated and entrained by secondary flows, providing less effectiveness near PS, near SS, and into the throat of the passage. The high momentum present for the high blowing rate, high-density coolant suppresses these secondary flows and provides enhanced cooling in the throat and in high secondary flow regions. However, the increased turbulence impartation due to lift off has an adverse effect on the heat load in the upstream region of the passage. It is concluded that only marginal gains near the throat of the passage are observed with an increase in high density coolant blowing rate, but severe thermal penalty is observed near the passage onset. / Master of Science / Gas turbine technology is used frequently in the burning of natural gas for power production. Increases in engine efficiency are observed with increasing firing temperatures, however this leads to the potential of overheating in the stages following. To prevent failure or melting of components, cooler air is extracted from the upstream compressor section and used to cool these components through various highly complex cooling schemes. The design and operational adequacy of these schemes is highly subject to the mainstream and coolant flow conditions, which are hard to represent in a laboratory setting. This experimental study explores the effects of various coolant conditions, and their respective response, for a purge jet cooling scheme commonly found in engine. This scheme utilizes two rows of staggered cylindrical holes to inject air into the mainstream from platform, upstream of the nozzle guide vane. It is the hope that this air forms a protective layer, effectively shielding the platform from the hostile mainstream conditions. Currently, little research has been done to quantify these effects of purge flow cooling scheme while mimicking engine geometry, mainstream and coolant conditions. For this study, an endwall geometry like that found in engine with a purge jet cooling scheme is studied. Commonly, an upstream gap is formed between the combustor lining and first stage vane platform, which is accounted for in this testing. Mainstream and coolant flow conditions can have large impacts on the results gathered, so both were matched to engine conditions. Varying of coolant density and injection rate is studied and quantitative results are gathered. Results indicate coolant fluid density plays a large role in purge jet cooling, and with neglection of this, potential thermal failure points could be overlooked This is exacerbated with less coolant injection. Interestingly, increasing the amount of coolant injected decreases performance across much of the passage, with only marginal gains in regions of complex flow. These results help to better explain the impacts of experimental neglect of coolant density, and aid in the understanding of purge jet coolant injection.