No description available.

No description available.

No description available.

La recherche de sources d’énergie fiables ayant un faible coût environnemental est en plein essor. L’hydrogène, étant un transporteur d’énergie propre et simple, pourrait servir comme moyen de transport de l’énergie de l’avenir. Une solution idéale pour les besoins énergétiques implique une production renouvelable de l’hydrogène. Parmi les possibilités pour un tel processus, la production biologique de l’hydrogène, aussi appelée biohydrogène, est une excellente alternative. L’hydrogène est le produit de plusieurs voies métaboliques bactériennes mais le rendement de la conversion de substrat en hydrogène est généralement faible, empêchant ainsi le développement d’un processus pratique de production d’hydrogène. Par exemple, lorsque l’hydrogène est produit par la nitrogénase sous des conditions de photofermentation, chaque molécule d’hydrogène constituée requiert 4 ATP, ce qui rend le processus inefficace. Les bactéries photosynthétiques non sulfureuses ont la capacité de croître sous différentes conditions. Selon des études génomiques, Rhodospirillum rubrum et Rhodopseudomonas palustris possèdent une hydrogénase FeFe qui leur permettrait de produire de l’hydrogène par fermentation anaérobie de manière très efficace. Il existe cependant très peu d’information sur la régulation de la synthèse de cette hydrogénase ainsi que sur les voies de fermentation dont elle fait partie. Une surexpression de cette enzyme permettrait potentiellement d’améliorer le rendement de production d’hydrogène. Cette étude vise à en apprendre davantage sur cette enzyme en tentant la surexpression de cette dernière dans les conditions favorisant la production d’hydrogène. L’utilisation de résidus organiques comme substrat pour la production d’hydrogène sera aussi étudiée. / The search for alternative energy sources with low environmental impact is in great expansion. Hydrogen, an elegant and simple energy transporter, could serve as means of transporting energy in the future. An ideal solution to the increasing energy needs would imply a renewable production of hydrogen. Out of all the existing possibilities for such a process, the biological production of hydrogen, also called biohydrogen, is an excellent alternative. Hydrogen is the end result or co-product of many pathways in bacterial metabolism. However, such pathways usually show low yields of substrate to hydrogen conversion, which prevents the development of efficient production processes. For example, when hydrogen is produced via nitrogenase under photofermentation conditions, each hydrogen molecule produced requires 4 molecules of ATP, rendering the process very energetically inefficient. Purple non-sulfur bacteria are highly adaptive organisms that can grow under various conditions. According to recent genomic analyses, Rhodospirillum rubrum and Rhodopseudomonas palustris possess, within their genome, an FeFe hydrogenase that would allow them to produce hydrogen via dark fermentation quite efficiently. Unfortunately, very little information is known on the regulation of the synthesis of this enzyme or the various pathways that require it. An overexpression of this hydrogenase could potentially increase the yields of substrate to hydrogen conversion. This study aims to increase our knowledge about this FeFe hydrogenase by overexpressing it in conditions that facilitate the production of hydrogen. The use of organic waste as substrate for hydrogen production will also be studied.

O objetivo deste trabalho foi enriquecer consórcio microbiano a partir de mistura de lodo granular de digestor anaeróbio de fluxo ascendente sob condições fototróficas anoxigênicas. Por meio de técnica de biologia molecular foi possível identificar 17 unidades taxonômicas operacionais (UTO) no consórcio microbiano, dentre as quais seqüências similares a Rhodobacter, gênero amplamente citado nos estudos de produção de gás hidrogênio por bactérias fototróficas. Exames microscópicos do consórcio fototrófico indicaram predomínio de bacilos Gram-negativos. Ensaios sob condições fototróficas foram realizados com dois meios de cultivo (RCVB e FANG) e os seguintes substratos orgânicos: ácido acético, butírico, cítrico, lático e málico, empregados como fonte de carbono, tanto para o crescimento celular, como para a produção do gás hidrogênio. A relação C/N inicial foi 30/4 e posteriormente 15/2, com o objetivo de favorecer o crescimento celular e a produção do \'H IND.2\'. A concentração dos substratos foi determinada de forma com que essa relação se mantivesse a mesma. O crescimento celular e consumo dos ácidos orgânicos foram similares para os dois meios de cultivo empregados. Entretanto, a produção do gás hidrogênio foi maior nos ensaios com o meio FANG. Dentre os substratos utilizados o consumo dos ácidos cítrico e málico foram os maiores (~100%), para concentrações iniciais de 3,3 g/L e 2,6 g/L, respectivamente. O menor consumo 25% foi observado em meio RCVB e ácido acético (2,5 g/L). O crescimento da biomassa variou de 0,06 g/L a 1,1 g/L, enquanto que a velocidade máxima específica de crescimento variou de 0,4 a 0,2 g SSV/L.d entre os substratos utilizados. A menor e maior concentração de hidrogênio foram de 8,5 e 22 mmol \'H IND.2\'/L, para os reatores alimentados com ácido lático e málico em meio FANG, respectivamente. Pôde-se concluir que o consórcio fototrófico enriquecido foi capaz de utilizar os ácidos orgânicos para produção do gás hidrogênio. / The aim of this work was enrich a mixture of granular sludge of an up flow anaerobic sludge blanket (UASB) under anoxygenic phototrophic conditions. The techniques of molecular biology identified 17 operational taxonomic units (UTO) in the microbial consortium among the sequences analised, which were similar to Rhodobacter, genus widely cited in studies of hydrogen gas production by phototrophic bacteria. Microscopic examinations of the phototrophic consortium showed predominance of Gram-negative bacilli. Tests were conducted under phototrophic conditions with two culture media (RCVB and FANG) and the following organic substrates: acetic, butyric, citric, lactic and malic acids that were used as carbon source for both cell growth and for the hydrogen gas production. The carbon nitrogen ratio (C/N) in the preliminaries tests was 30/4 and then it was changed to15/2 in order to improve the cell growth and hydrogen production. The concentration of substrates was determined for remain the same carbon/nitrogen ratio among the substrates. The cell growth and consumption of organic acids were similar for the two culture media used. However, the production of hydrogen gas was higher in trials with the medium FANG. Among the substrates used, the consumption of malic and citric acids were the highest (~100%) for initial concentrations of 3.3 g/L and 2.6 g/L, respectively. The shortest consumption (25%) was observed for the cells that grew on acetic acid, 2.5 g/L in RCVB culture medium. The growth of the biomass varied from 0.06 g/L to 1.1 g/L, whereas the maximum specific growth rate ranged from 0.4 to 0.2 g VSS/L.d between the substrates used. The lowest and highest concentrations of hydrogen were 8.5 and 22 mmol \'H IND.2\'/L for the reactor fed with lactic acid and malic acid in FANG\'s medium, respectively. It was concluded that the phototrophic consortium was able to use those organic acids for the production of hydrogen gas.

Em função do número reduzido de estudos sobre o movimento da água e de íons no bulbo molhado, foi realizado este trabalho com o objetivo de avaliar a distribuição de água e concentração de nutrientes no bulbo formado pelo sistema de gotejamento enterrado em comparação ao superficial. O ensaio foi conduzido na área experimental do Departamento de Engenharia Rural da Escola Superior de Agricultura "Luiz de Queiroz" - ESALQ/USP, localizada no município de Piracicaba, SP. Para a avaliação foram abertas trincheiras e instaladas sondas de TDR, para o monitoramento da distribuição da solução, dispostas a 0,5; 0,15; 0,25; 0,35 e 0,45 m - profundidade, e a 0,5; 0,15; 0,25; 0,35 m - comprimento, totalizando 17 sondas em malha por trincheira. A solução de KNO3 foi aplicada a partir de gotejadores com diferentes vazões (2 e 4 L/h), sendo aplicado o volume de 1 L em intervalos de 1 hora, totalizando 10 L de solução por repetição, Conjuntamente ao monitoramento da distribuição da solução no solo, medições da área superficial molhada e do disco saturado foram feitas com régua milimetrada, além disso, foi estabelecido um volume de controle onde foram avaliadas a uniformidade de aplicação de água e KNO3. Desta forma, foi possível verificar que: em relação aos sistemas superficiais, os sistemas enterrados apresentaram menor área superficial molhada e atingiram uma maior largura e profundidade; a umidade inicial do solo influencia a formação do bulbo molhado e o movimento do KNO3 no solo; a uniformidade de sais distribuídos no volume de controle é afetada negativamente pelo aumento do volume aplicado e as maiores concentrações de água e concentração de KNO3 foram obtidas próximas ao ponto de emissão. / In function of the reduced number of studies on the water and ions movement in the wet soil volume, this work was conducted with the goal of evaluating the distribution of water and nutrient concentration in the wetted soil volume formed by subsurface drip irrigation (SDI) in comparison to surface system. The rehearsal was concducted in the experimental area of the Rural Engineering Department - ESALQ/USP, located in Piracicaba, SP. Trenches were opened and installed probes of TDR for studying to solution distribution, to facilitate the study a mesh of probes was disposed to 0,5; 0,15; 0,25; 0,35 and 0,45 m - depth, and to 0,5; 0,15; 0,25; 0,35 m - length, with the total of 17 probes. The solution of KNO3 was applied starting from emitters with different flows (2 and 4 L/h), it was applied the volume of 1 L within intervals of 1 hour, with the total of 10 L of solution for repetition. All together to study the solution distribution in the soil, the wet superficial area measurements and of the saturated disc they were done by the ruler, besides, it was established a control volume where the uniformity application of water and KNO3 was analysed. This way, it was possible to verify that: in relation to the superface systems, the SDI presented smaller wet superficial area and they reached a larger width and depth; the initial soil water content influences the formation of the wetted soil volume and the KNO3 movement in the soil; the salt uniformity, distributed in the control volume, is affected negatively by the applied volume increased, and the largest concentrations of water and KNO3 were obtained close to the emission point.

"An apparatus for the delivery of radiation to the tip-sample interface of an Atomic Force Microscope (AFM) is demonstrated. The Pulsed Light Delivery System (PLDS) was fabricated to probe photoinduced conformational changes of molecules using an AFM. The PLDS is 67 mm long, 59 mm wide, and 21 mm high, leaving clearance to mount the PLDS and a microscope slide coated with a thin film of photoactive molecules beneath the cantilever tip of a stand-alone AFM. The PLDS is coupled into a fiber pigtailed Nd:Yag frequency doubled laser, operating at a wavelength of 532 nm. The radiation delivered to a sample through the PLDS can be configured for continuous or pulsed mode. The maximum continuous wave (CW) power delivered was 0.903 mW and the minimum pulse width was 12.3 ms (maximal 401 ms), corresponding to a minimal energy of 0.150 nJ (maximal 362 nJ), and had a cycle duration of 10.0 ms. The PLDS consists of micro-optical components 3.0 mm and smaller in diameter. The optical design was inspired by the three-beam pick-up method used in CD players, which could provide a method to focus the pulse of light onto the sample layer. In addition, the system can be easily modified for different operational parameters (pulse width, wavelength, and power). As proof that the prototype design works, we observed a photoinduced ‘bimetallic’ bending of the cantilever, as evidenced by observing no photoinduced bending when a reflective-coated cantilever was replaced by an uncoated cantilever. Using the apparatus will allow investigation of many different types of molecules exhibiting photoinduced isomerization."

The purpose of this thesis is to assess the extent to which the singular readings of a manuscript reveal the tendencies of the scribe who wrote its text by examining three related Greek manuscripts from the sixth century. The three manuscripts are all luxury copies of the Gospels' purple codices, so named because they are written in silver and gold ink on parchment that has been dyed purple. The manuscripts, Codex Purpureus Petropolitanus (N 022), Codex Sinopensis (O 023) and Codex Rossanensis (Σ 042), were all copied in the sixth century from a common exemplar. Chapter One introduces the three manuscripts. Chapter Two provides a history of research on scribal habits and singular readings, and it describes the method used in this thesis to determine both the validity of the singular readings method and the actual scribal habits of 022, 023 and 042. Chapter Three provides a preliminary assessment of each scribe by comparing scribal features in the passages extant in all three manuscripts. Chapters Four, Five and Six assess the scribal habits of 022, 023 and 042, respectively. In these chapters, perceived scribal habits are measured by a modified singular readings method to replicate the situation for each manuscript if it had no extant close relatives' the situation for most early manuscripts. Actual scribal habits are then determined by the places the scribe changed the text of the exemplar. Chapter Seven offers some concluding thoughts about the scribes, their exemplar and the use of singular readings to determine scribal habits. Appendix One presents for the first time an edition of the reconstructed text of the exemplar of 022, 023 and 042, where at least two of the three manuscripts are extant. Appendices Two, Three and Four are full transcriptions of the Gospel of Matthew in 022, 023 and 042, respectively. Appendix Five provides information on singular readings and corrections in 042 where it alone is extant of the three manuscripts. Appendix Six describes the codicological structures of the three manuscripts. Appendix Seven is a transcription and brief discussion of 080, a fragmentary of a purple codex dating to the sixth century. Finally, both 022 and 042 contain a series of secondary corrections made against a second exemplar, and Appendix Eight argues that the scribe of 042 was responsible for these corrections in both manuscripts.

Made available in DSpace on 2015-04-22T22:17:26Z (GMT). No. of bitstreams: 1 Antonia Paiva Teixeira.pdf: 430157 bytes, checksum: d5512cf8160a1460ce30ab984f83a8a0 (MD5) Previous issue date: 2011-02-21 / Fundação de Amparo à Pesquisa do Estado do Amazonas / The aim of this study was to evaluate the potential of purple yam (Dioscorea trifida), as possible health-promoting ingredient in bread making, in the state of Amazonas. Thus, we determined the centesimal composition, energy, and antioxidant activity of purple yam and four formulations of purple yam bread (0%, 10%, 15% and 20%). We also performed an acceptance test and microbiological analysis of the formulations 10%, 15% and 20%. The Kruskal-Wallis ANOVA revealed no significant differences for the parameters of the centesimal composition and energy of the four formulations, except for lipid. That is, in the process of purple yam bread making, an addition of purple yam in natura up to 20%, instead of wheat flour in ordinary bread (0%), can be made with no effect on the diet s energy, since their chemical composition are similar. By the DPPH method, the samples of purple yam revealed a high antioxidant activity, with values exceeding 70%. The three purple yam breads showed some antioxidant activity with the maximum values above 40%. The LPO method confirmed the antioxidant activity in purple yam and in purple yam breads. In both methods (DPPH and LPO), the antioxidant activity increased as the ratio of purple yam tubers, in substitution of wheat flour, was incorporated in the breads. The Friedman ANOVA was applied to compare the three formulations of purple yam breads; a significant difference only in the attribute color was revealed in the acceptance test. The 20% bread received the best evaluation; the more purple yam in the breads, the higher was the average score. Considering that, for the other sensory attributes, there were no preference differences detected among the breads, they can be considered approved. The results suggest the feasibility of purple yam bread consumption, and its possible use as a health-promoting food. / O objetivo desse estudo foi avaliar o potencial do cará-roxo (Dioscorea trifida) no estado do Amazonas como ingrediente funcional na fabricação de pães. Desse modo, foram determinadas a composição centesimal, valor calórico, e atividade antioxidante do cará-roxo e de quatro formulações de pães de cará-roxo (0%, 10%, 15% e 20%). Foram realizados também teste de aceitação e análise microbiológica das formulações 10%, 15% e 20%. A ANOVA de Kruskal-Wallis não revelou diferença significante para os parâmetros da composição centesimal e valor calórico das quatro formulações, a exceção do lipídio. Isto é, na fabricação de pão de cará-roxo, uma adição de cará-roxo in natura até 20%, em substituição à farinha de trigo no pão comum (0%), pode ser feita sem afetar o valor calórico da dieta, uma vez que a composição centesimal entre os pães é semelhante. As amostras de cará-roxo revelaram uma elevada atividade antioxidante pelo método de sequestro de radicais livres (2,2-difenil-1-picrilhidrazila-DPPH), com valores superiores a 70%. Os pães de cará-roxo (10%, 15% e 20%) apresentaram certa atividade antioxidante com os valores máximos acima de 40%. O método da lipoperoxidação (LPO) confirmou a atividade antioxidante no cará-roxo e nos pães de cará-roxo. Em ambos os métodos (DPPH e LPO), a atividade antioxidante aumentou na proporção em que os tubérculos do cará-roxo foram incorporados ao pão, em substituição à farinha de trigo. A ANOVA de postos de Friedman aplicada para comparar as três formulações de pães de cará-roxo, no teste de aceitação revelou diferenças significantes apenas para o atributo cor. O pão a 20% apresentou uma melhor avaliação. Quanto mais cará-roxo nos pães, maior a nota média obtida. Considerando que, para os demais atributos sensoriais não foram detectadas diferenças de preferência entre os pães, estes podem ser considerados aprovados. Os resultados sugerem a viabilidade do consumo de pão à base de cará-roxo, e seu uso como alimento funcional.

Made available in DSpace on 2017-07-10T17:36:55Z (GMT). No. of bitstreams: 1 2014_Diss_Paulo_Ricardo_Lima.pdf: 1308778 bytes, checksum: fcc1fc80a1ef2f3cfecb3a398ecc76bb (MD5) Previous issue date: 2014-02-21 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The knowledge of Morphophysiological mechanisms that allow a plant tolerate water stress conditions constitutes important tool for analyzing the quality of seedlings, so it the work aimed to quantify the effects of water management in the period of rustification in seedlings of Handroanthus impetiginosus (Mart. ex DC.) Mattos and Gallesia integrifolia (Spreng.) Harms by means of foliar thermometry and parameters morphometric of quality and additionally analyze the performance of seedlings after the rustification, in soil sandy and loamy soil. The experiment was conducted in a protected ambient at the State University of West Paraná, campus Marechal Cândido Rondon - PR. The treatments were comprised of four hydric regimes: daily irrigation (control), every two, three and four days in 60 seedlings per treatment for four weeks. The mensurations involved the measurement of the leaf temperature (TF), the increments in height, the stem diameter, in the mass dry of root , in the mass dry of shoot and leaf area. The mensurations of TF occurred at the end of each irrigation management cycle and for the other variables were held measurements at the beginning and end of rustification. After the impositions of hydric managements, the seedlings were taken to pots containing sandy soil and clay soil to check the influence of different hydric management as well as soil type in the seedling survival. The analysis between the control treatment and irrigation every two days resulted in significant differences (P> 0,05) merely for leaf area, whereas in and both species studied presented decreased of leaf area with the increase of hydric restriction. Seedlings of H. impetiginosus with of irrigation cycles every three days, showed a reduction in the rate of growth in diameter of 29%, of 58% to increment the dry mass of shoots and 32% for leaf area compared to the management control. Seedlings of G. integrifolia irrigated every three days showed no significant differences (P> 0,05) compared with irrigated daily. The irrigation management every four days in seedlings of H. impetiginosus enabled higher speed of growth of the root system and reduced aerial growth, resulting in warming the leaf, whereas the irrigation management every four days in seedlings of G. integrifolia presented reduction in the rate of growth of the shoot and not presented difference (P> 0,05) in the root system growth and also resulted in leaf warming. The use of the infrared thermometry allows benchmarking the leaf temperature during rustification of seedlings of H. impetiginosus and G. integrifolia. Was no influence of soils in the seedling survival of H. impetiginosus and G. integrifolia, and symptoms of hydric deficit always if manifested first in clay soil, mainly in seedlings not adapted to hydric stress. In both species, the symptoms of hydric deficit presented by the seedlings of different management hydrics showed that seedlings submitted to the irrigation regimes every three and four days, were attenuating slowly the symptoms of hydric deficit compared with others hydrics management / O conhecimento dos mecanismos morfofisiológicos que permitem a uma planta tolerar as condições de estresse hídrico constitui importante ferramenta para analisar a qualidade de mudas. O trabalho objetivou quantificar os efeitos do manejo hídrico no período de rustificação em mudas de Handroanthus impetiginosus (Mart. ex DC.) Mattos e Gallesia integrifolia (Spreng.) Harms por meio da termometria foliar e por parâmetros morfométricos de qualidade e adicionalmente analisar o desempenho das mudas após a rustificação, em solo arenoso e argiloso. Conduziu-se o experimento em ambiente protegido na Universidade Estadual do Oeste do Paraná, campus de Marechal Cândido Rondon PR. Os tratamentos foram compostos por quatro regimes hídricos: irrigação diária (tratamento controle), a cada dois, três e a cada quatro dias, em 60 mudas por tratamento, durante quatro semanas. As mensurações envolveram a medição da temperatura foliar (TF), os incrementos na altura, no diâmetro de colo, na massa seca radicular, na massa seca da parte aérea e na área foliar. As mensurações da TF ocorreram ao final do ciclo de cada manejo de irrigação e para as demais variáveis realizaram-se as mensurações no início e final da rustificação. Após a imposição dos manejos hídricos, as mudas foram levadas para vasos contendo solo arenoso e argiloso, para verificar se há influencia dos diferentes manejos hídricos, bem como do tipo do solo na sobrevivência da muda. A análise entre os tratamentos controle e irrigação a cada dois dias resultou em diferenças (P> 0,05) apenas para área foliar, sendo que ambas as espécies estudadas apresentaram redução da área foliar com o aumento da restrição hídrica. Mudas de H. impetiginosus com ciclos de irrigação a cada três dias, apresentou redução na taxa de crescimento em diâmetro de 29%, de 58% para incremento da massa seca da parte aérea e de 32% para área foliar em comparação ao manejo controle. Mudas de G. integrifolia irrigadas a cada três dias não apresentaram diferenças significativas (P> 0,05) em relação às irrigadas diariamente. O manejo de irrigação a cada quatro dias em mudas de H. impetiginosus possibilitou maior velocidade de crescimento do sistema radicular e reduziu o crescimento aéreo, resultando no aquecimento foliar, enquanto que o manejo de irrigação a cada quatro dias em mudas de G. integrifolia apresentou redução na velocidade de crescimento da parte aérea e não apresentou diferença (P> 0,05) no crescimento do sistema radicular e também resultou no aquecimento foliar. O uso da termometria infravermelho permite aferir a temperatura foliar na rustificação de mudas de H. impetiginosus e G. integrifolia. Houve influência dos solos na sobrevivência das mudas de H. impetiginosus e G. integrifolia, e os sintomas de déficit hídrico sempre se manifestaram primeiro no solo argiloso, principalmente nas mudas não adaptadas ao estresse hídrico. Em ambas as espécies, as diferenças dos sintomas de déficit hídrico apresentadas pelas mudas dos diferentes manejos hídricos mostraram que mudas submetidas ao regime de irrigação a cada três e quatro dias, foram atenuando lentamente os sintomas de déficit hídrico em comparação aos demais manejos hídricos