As bactérias fototróficas freqüentemente apresentam florescimentos em lagoas de estabilização utilizadas no tratamento de esgoto sanitário, formando uma camada de cor púrpura na sua superfície. Portanto, o estudo das condições que propiciam tais florescimentos, a diversidade microbiana, o potencial de remoção da matéria orgânica e o estabelecimento das relações entre tais conhecimentos, permitem compreender o metabolismo do sistema. Nesse sentido, o objetivo deste trabalho foi avaliar a diversidade de bactérias (domínio Bacteria), bactérias fototróficas púrpuras e bactérias redutoras de sulfato (BRS) em lagoas de estabilização do Vale do Ribeira (Cajati, SP). Para tal, foram realizadas coletas sazonais (primavera, verão, outono e inverno) na sub-superfície, camada intermediária e interface água-sedimento, em dois horários (14:00 h e 02:00 h), nas lagoas anaeróbia e facultativa. Para analisar os diferentes grupos de microrganismos, utilizou-se a técnica de PCR/DGGE, com primers específicos. Nas análises de filogenia realizou-se o seqüenciamento parcial do gene RNAr 16S e da subunidade M do centro de reação fotossintético das bactérias fototróficas púrpuras. Análises físico-químicas, tais como sulfato, DQO, sólidos, nitrogênio e fósforo foram realizadas, além da determinação da concentração de oxigênio dissolvido, pH, temperatura e radiação solar fotossinteticamente ativa incidente. No outono observou-se maior diversidade de microrganismos do domínio Bacteria, bactérias fototróficas púrpuras e BRS, enquanto na primavera foi verificada a menor diversidade desses microrganismos para as duas lagoas. Na lagoa facultativa foi observada maior diversidade do domínio Bacteria e das BRS em relação à lagoa anaeróbia. Verificou-se maior diversidade de bactérias fototróficas púrpuras na lagoa anaeróbia, caracterizada por duas populações predominantes nas quatro estações e nas diferentes profundidades. A concentração de matéria orgânica (DQO) variou de 60,3 mg/L (inverno) a 298,0 mg/L (primavera) e a maior concentração de sulfato observada foi de 51,0 mg/L (inverno). Bacilo curvo Gram negativo, semelhante à bactéria fototrófica púrpura não sulfurosa, presente em amostra proveniente da sub-superfície da lagoa anaeróbia foi purificado e apresentou 92% de similaridade com Rhodopseudomonas palustris. Em ambas as lagoas foram identificadas bactérias semelhantes a Chromobacterium suttsuga (95%), Clostridium sp. (99%), Rhodobacter sphaeroides (99%), Rhodopseudomonas palustris (99%), Lampropedia hyalina (97%), Campylobacter fetus (99%), Desulfovibrio vulgaris (95%), Rhodospirillum rubrum (95%) e diferentes bactérias não cultivadas. / The phototrophic bacteria frequently blossom in the stabilization lagoons that are used in sanitary sewer treatment, forming a purple layer on its surface. Therefore, the study of the conditions that propitiate such blooms, the microbial diversity, the removal of the organic matter and the establishment of the relations between them permit to understand the metabolism of the system. The objective of this work was to evaluate the diversity of the bacteria (Bacteria domain), purple phototrophic bacteria and sulfate reducing bacteria (SRB) in stabilization lagoons of Vale do Ribeira (Cajati - SP). For this, it was made seasonal collects (spring, summer, autumn and winter) from the sub-surface, intermediate layer and interface water-sediment, at two times (14:00 h and 02:00 h) of the anaerobic and facultative lagoons. To analyze the different groups of microorganisms it was used the PCR/DGGE technique, with specific primers; for the phylogenic analysis it was realized the DNA partial sequencing of the 16S RNAr gene and of the subunit M of the photosynthetic center of reaction of the purple photosynthetic bacteria. It was determined: the concentration of dissolved oxygen, pH, temperature and photosynthetically active incident solar radiation, and the physical-chemistry analysis as: COD, solids, nitrogen and phosphorus. In the autumn it was observed greater diversity of microorganisms of the Bacteria domain, the group of the purples phototrophic bacteria and SRB, while in the spring it was verified minor diversity of these microorganisms in the two lagoons studied. In the facultative lagoon it was observed greater diversity of the Bacteria domain and of the SRB with respect to the anaerobic lagoon. It was verified greater diversity of the purple phototrophic bacteria in the anaerobic lagoon, of what in the facultative lagoon, which was characterized by the two predominant populations in the four seasons and in the different points of collect. The concentration of the organic matter (COD) varied from 60,3 mg/L (winter) to 298,0 mg/L (spring) and the greater concentration of sulfate observed was of 51,0 mg/L (winter). Arched bacillus Gram-negative similar to purple not sulfurous bacteria, from a sample of the sub-surface of the anaerobic lagoon was purified and presented 92% of similarity with Rhodopseudomonas palustris. In both lagoons it was identified bacteria similar to Chromobacterium suttsuga (95%), Clostridium sp. (99%), Rhodobacter sphaeroides (99%), Rhodopseudomonas palustris (99%), Lampropedia hyalina (97%), Campylobacter fetus (99%), Desulfovibrio vulgaris (95%), Rhodospirillum rubrum (95%).

Bildungsroman in Contemporary Black Women's Fiction is a study of Toni Morrison's The Bluest Eye and Alice Walker's The Color Purple. Both of these writers implement a newer version of the genre of Bildungsroman to reveal the complexities involved in coming of age for a young woman of color. Both novels have protagonists that struggle with racism, sexism, and classism as barriers to their identity formation. This study aims to reveal the ways in which multiple layers of oppression inhibit the progress of contemporary African-American female heroines in modem Bildungsroman. / Graduation date: 2002

The protagonists in the fiction of Paule Marshall, Alice Walker, and Toni Morrison illuminate American cultural perceptions of black women and illustrate how the creators of these characters hope to change those perceptions. I studied Paule Marshall's Daughters, Alice Walker's Meridian and The Color Purple, and Toni Morrison's The Bluest Eye to learn what the writers of these novels have to say about the women they hope black girls can grow up to be and to learn what potential for self-development they see for black women. For example, in order to become whole people, what do black girls and black women need from their parents and their community? What do black women need from their intimate relationships? "Part One: Political, Historical and Religious Identity " surveys politics, religion and history for views of black women. Politically, they appear disenfranchised; historically they were property. In reference to religion, I found that a white male religion does not serve black women well. Walker sees god within her female protagonist Celie, and Marshall has a belief in a Caribbean/African diaspora that provides a sense of spiritual and cultural continuity. "Part Two: Childhood Identity" explores childhood and the community's role. Childhood appears as a critical time for self-development. The adults in the community contribute to the child's self-awareness. Mistreatment of girls causes them harm throughout their lives. How well the community safeguards its children is a measure of how highly these children are valued. These authors want to see girls more highly regarded. Toward this end, they expose the abuse that takes place in the community. Morrison shows not only the abuse, but also the love. By showing concerned parents as well as neglectful ones, Morrison offers a fuller portrait of the community she knows. The Color Purple also tells a story of sexual abuse of a girl, but this abuse is overcome by the inner strength of the victim combined with the loving support of Shug Avery and the supportive community context of the juke where Celie is accepted. The portrayal of childhood in Daughters involves a Caribbean island culture where the roles of the women that the child Ursa observes offer few role models. "Part Three: Adult Relational Identity" looks at the dilemma in communication between the sexes and across the generations from mother to daughter. Step-fathers and husbands are abusive characters in Walker's writing, while Morrison shows a loving father and an incestuous father in The Bluest Eye. "Part Four: Language Identity" discusses Black English, orality and dialect, looking at the role of language as an aspect of self-definition. James Baldwin's view of language is presented: rejecting a child's language is rejecting the child himself. Baldwin's view supports the attitude toward language as self-defining that appears in the writing of Marshall, Morrison, and Walker. These authors show pride in Black English, and they demonstrate their ability with edited English through their own writing. / Graduation date: 1994

La recherche de sources d’énergie fiables ayant un faible coût environnemental est en plein essor. L’hydrogène, étant un transporteur d’énergie propre et simple, pourrait servir comme moyen de transport de l’énergie de l’avenir. Une solution idéale pour les besoins énergétiques implique une production renouvelable de l’hydrogène. Parmi les possibilités pour un tel processus, la production biologique de l’hydrogène, aussi appelée biohydrogène, est une excellente alternative. L’hydrogène est le produit de plusieurs voies métaboliques bactériennes mais le rendement de la conversion de substrat en hydrogène est généralement faible, empêchant ainsi le développement d’un processus pratique de production d’hydrogène. Par exemple, lorsque l’hydrogène est produit par la nitrogénase sous des conditions de photofermentation, chaque molécule d’hydrogène constituée requiert 4 ATP, ce qui rend le processus inefficace. Les bactéries photosynthétiques non sulfureuses ont la capacité de croître sous différentes conditions. Selon des études génomiques, Rhodospirillum rubrum et Rhodopseudomonas palustris possèdent une hydrogénase FeFe qui leur permettrait de produire de l’hydrogène par fermentation anaérobie de manière très efficace. Il existe cependant très peu d’information sur la régulation de la synthèse de cette hydrogénase ainsi que sur les voies de fermentation dont elle fait partie. Une surexpression de cette enzyme permettrait potentiellement d’améliorer le rendement de production d’hydrogène. Cette étude vise à en apprendre davantage sur cette enzyme en tentant la surexpression de cette dernière dans les conditions favorisant la production d’hydrogène. L’utilisation de résidus organiques comme substrat pour la production d’hydrogène sera aussi étudiée. / The search for alternative energy sources with low environmental impact is in great expansion. Hydrogen, an elegant and simple energy transporter, could serve as means of transporting energy in the future. An ideal solution to the increasing energy needs would imply a renewable production of hydrogen. Out of all the existing possibilities for such a process, the biological production of hydrogen, also called biohydrogen, is an excellent alternative. Hydrogen is the end result or co-product of many pathways in bacterial metabolism. However, such pathways usually show low yields of substrate to hydrogen conversion, which prevents the development of efficient production processes. For example, when hydrogen is produced via nitrogenase under photofermentation conditions, each hydrogen molecule produced requires 4 molecules of ATP, rendering the process very energetically inefficient. Purple non-sulfur bacteria are highly adaptive organisms that can grow under various conditions. According to recent genomic analyses, Rhodospirillum rubrum and Rhodopseudomonas palustris possess, within their genome, an FeFe hydrogenase that would allow them to produce hydrogen via dark fermentation quite efficiently. Unfortunately, very little information is known on the regulation of the synthesis of this enzyme or the various pathways that require it. An overexpression of this hydrogenase could potentially increase the yields of substrate to hydrogen conversion. This study aims to increase our knowledge about this FeFe hydrogenase by overexpressing it in conditions that facilitate the production of hydrogen. The use of organic waste as substrate for hydrogen production will also be studied.

Thesis (MA (English))--University of Stellenbosch, 2009. / ENGLISH ABTRACT: In recent years, original and exciting developments have been taking place in Nigerian literature. This new body of literature, collectively referred to as the ―third generation‖, has lately received international acclaim. In this emergent literature, the negotiation of a new, contemporary identity has become a central focus. At the same time, recent Nigerian literary texts are articulating responses to various developments in the Nigerian nation: Nigeria‘s current political and socio-economic situation, diverse forms of cultural hybridisation, as well as an increasing trans-national consciousness, to mention only a few. Three 21st-century novels – Chimamanda Nogzi Adichie‘s Purple Hibiscus (2004), Sefi Atta‘s Everything Good Will Come (2004) and Chris Abani‘s GraceLand (2005) – reveal how new avenues of identity-negotiation and formation are being explored in various contemporary Nigerian situations. This study tracks the ways in which the Bildungsroman, the novel of self-development, serves as a vehicle through which this new identity is articulated. Concurrently, this study also grapples with the ways in which the articulation and negotiation of this new identity reshapes the conventions of the classical Bildungsroman genre, thereby establishing a unique and contemporary Nigerian Bildungsroman for the 21st century. The identity that is being negotiated by the third generation is multi-layered and inclusive, as opposed to the exclusive and unitary identities which are observable in Nigerian novels of the previous two generations. Such inclusivity, as well as the hybrid environments in which this identity is being negotiated, results in a form of ―identity layering‖. Thus, the individual comes into being at the point of intersection, overlap and collision of various modes of self-making. Such ―layering‖ allows the individual, albeit not without challenge, to perform a self-styled identity, which does not necessarily conform to the dictates of society. At the same time, the identity is negotiated by means of an engagement, in the form of intertextual dialoguing, with Nigeria‘s preceding literary generations. The most prominent arenas in which this new identity is negotiated include silenced domestic spaces, religo-cultural traditions, constructs of gender and nation, as well as in multicultural and hybrid communities. The investigation conducted in this thesis will, consequently, also focus on such areas of Nigerian life, as they are portrayed in the focal texts. Various theories of literary analysis (some of which specifically focus on Nigeria), Bildungsroman theory, theories of allegory, (imaginative) nation formation, feminism, gender and performativity, as well as theories of cultural identity and cultural exchanges, will form the critical and theoretical framework within which this investigation will be executed. Chapter One explores how Purple Hibiscus‘s protagonist, Kambili Achike, negotiates her gender identity and voice in order to constitute herself as an independent, self-authoring individual. Chapter Two, which focuses on Everything Good Will Come, investigates the dialectic relationship between Enitan Taiwo‘s national and personal identity, which inevitably leads to her quest to reconceive her gender identity, since national identity, as she finds out, is always an engendered construct. In its analysis of GraceLand, Chapter Three turns to the difficulties that Elvis Oke faces when he attempts to negotiate an alternative masculine identity within a rigid patriarchal system and between the cracks of a fraudulent African modernity. / AFRIKAANSE OPSOMMING: In die afgelope paar jaar was daar opwindende, oorspronklike ontwikkelinge in Nigeriese literatuur. Hierdie nuwe literatuurkorpus, wat gesamentlik bekend staan as die ―derde generasie, het onlangs internasionale erkenning ontvang. In hierdie opkomende literatuur, kry die soeke na 'n nuwe, kontemporêre identiteit ‘n sentrale fokus. Terselfdertyd reageer onlangse Nigeriese literêre werke met verskeie ontwikkelinge in die Negeriese nasie: Nigerië se huidige politieke en sosio-ekonomiese situasie, diverse vorme van kultuurverbastering asook 'n toenemende trans-nasionale bewustheid, om maar ‘n paar te noem. Drie 21ste eeuse romans – Chimamanda Nogzi Adichie se Purple Hibiscus (2004), Sefi Atta se Everything Good Will Come (2004) en Chris Abani se GraceLand (2005) – onthul hoe nuwe kanale van identiteidsonderhandeling en –vorming in verskeie kontemporêre Nigeriese situasies ondersoek word. Hierdie studie ondersoek die maniere waarop die Bildungsroman, die roman van selfontwikkeling, as ‗n medium dien waardeur hierdie nuwe identiteit geartikuleer word. Terselfdertyd sal hierdie studie ook worstel met die maniere waarin die artikulasie en soeke na hierdie nuwe identiteit die konvensies van die klassieke Bildungsroman genre hervorm, en daardeur 'n unieke en kontemporêre Nigeriese Bildungsroman vir die 21ste eeu vestig. Die identiteit wat ontwikkel deur die derde generasie is veelvlakkig en inklusief en staan teenoor die eksklusiewe, eenvormige identiteite wat in Nigeriese romans van die vorige twee generasies opgemerk word. Hierdie inklusiwiteit, sowel as die hibriede omgewings waarin hierdie identeite ontwikkel word, lei tot die vorming van identiteitslae. Die individu kom dus tot stand by die kruising, oorvleueling en botsing van verskillende metodes van selfvorming. Hierdie vorming van lae laat die individu toe, alhoewel nie sonder uitdagings nie, om 'n selfgevormde identiteit te hê wat nie noodwndig aan die eise van die gemeenskap voldoen nie. Terselfdertyd word hierdie identiteit onderhandel deur ‗n skakeling met Nigerië se voorafgaande literêre generasies in die vorm van intertekstuele dialoog. Die mees prominente omgewings waar hierdie nuwe identiteit onderhandel word, sluit stilgemaakte huishoudelike spasies, religieus-kulturele tradisies, konstrukte van gender en nasie, sowel as multi-kulturele en hibriede gemeenskappe in. Die ondersoek wat in hierdie tesis uitgevoer sal word, sal daarom ook fokus op hierdie areas van Nigeriese lewe, soos deur die fokale tekste voorgestel. Verskeie teorieë van literêre analise (sommige wat spesifiek op Nigerië fokus), Bildungsromanteorie, teorieë van allegorie, (denkbeeldige) nasievorming, feminisme, gender en performatiwiteit, sowel as teorieë van kultuuridentiteit en -uitruiling, vorm die kritiese en teoretiese raamwerk waarbinne hierdie ondersoek uitgevoer sal word. Hoofstuk een ondersoek hoe Purple Hibiscus se protagonist, Kambili Achike, haar genderidentiteit onderhandel en uitdrukking gee om haarself as onafhanklike, self-skeppende individu te vorm. Hoofstuk twee, wat fokus op Everything Good Will Come, ondersoek die dialektiese verhouding tussen Enitan Taiwo se nasionale en persoonlike identiteit, wat onvermydelik lei tot die herbedenking van haar genderidentiteit, aangesien nasionale identiteit, soos sy uitvind, altyd 'n gekweekte konstruk is. In sy analise van GraceLand, draai Hoofstuk drie om die moeilikhede wat Elvis Oke in die gesig staar wanneer hy probeer om ‘n alternatiewe manlike identiteit te onderhandel in 'n rigiede patriargale sisteem tussen krake van 'n bedrieglike Afrika-moderniteit.

Mercury (Hg) is a global pollutant and potent neurotoxin that bioaccumulates in aquatic and terrestrial food webs as monomethylmercury (MeHg). Anaerobic microbes are largely responsible for MeHg production, which depends on the bioavailability of inorganic Hg substrates to methylators. Hg redox cycling pathways such as Hg reduction play a key role in determining Hg’s availability in the environment. Although abiotic photochemical Hg reduction typically dominates in oxic surface environments, Hg reduction pathways mediated by photosynthetic and anaerobic microbes are thought to play an important role in anoxic habitats where light is limited and MeHg production occurs. Currently, the physiological mechanisms driving phototrophic and anaerobic Hg reduction remain poorly understood. The main objective of my thesis is to provide mechanistic details on novel anaerobic and phototrophic Hg reduction pathways. I used a combination of physiological, biochemical and trace Hg analytical techniques to study Hg reduction pathways in a variety of anaerobic and photosynthetic bacteria. I demonstrated that Hg redox cycling was directly coupled to anoxygenic photosynthesis in aquatic purple non-sulphur bacteria that reduced HgII when cells incurred a redox imbalance. I discovered that terrestrial fermentative bacteria reduced Hg through pathways that relied on the generation of reduced redox cofactors. I also showed that sulphur assimilation controlled Hg reduction in an anoxygenic phototroph isolated from a rice paddy. In addition, I developed methods to explore cryptic anaerobic Hg redox cycling pathways using Hg stable isotope fractionation. At its core, my thesis underscores the intimate relationship between cell redox state and microbial Hg reduction and suggests a wide diversity of microbes can participate in anaerobic Hg redox cycling.

Submitted by Maike Costa (maiksebas@gmail.com) on 2017-02-13T12:49:06Z No. of bitstreams: 1 arquivototal.pdf: 4143600 bytes, checksum: 07d03f3ec93fc9d92195b8cd8296591d (MD5) / Made available in DSpace on 2017-02-13T12:49:06Z (GMT). No. of bitstreams: 1 arquivototal.pdf: 4143600 bytes, checksum: 07d03f3ec93fc9d92195b8cd8296591d (MD5) Previous issue date: 2015-02-09 / The use of sports supplements is something already well established in the sport, however recent years have been marked by the discovery of foods that demonstrate ergogenic activity similar to that of sports supplements. Several of these foods show up able to minimize oxidative stress, inflammation and muscle injury induced by intense training. The purple grapes and their derivatives have a particular antioxidant action and anti-inflammatory due to their composition rich in polyphenols, so that has been demonstrated substantial capacity to improve cardiometabolic parameters in hypertensive, diabetic, neurodegenerative and liver diseases. But yet few studies that confirmed the ergogenic capacity of grape juice in athletes. In this context, the present study aimed to investigate the effects of the integral purple grape juice supplementation on oxidative stress, inflammation, immune response, muscle injury and performance in recreational runners. Therefore an experimental study, randomized and controlled was conducted with 28 recreational runners. Two groups were configured: grape juice (GJG; n= 15; 42,7±8,1 anos) who ingested 10 ml/ kg/ day of integral purple grape juice and control group (GC, n= 13; 36,3±8,1 anos) consumed isocaloric carbohydrate beverage, isoglicidic and isovolumetric daily for 28 days. The parameters evaluated were total antioxidant capacity - TAC, vitamins A and E, uric acid and malondialdehyde-MDA (oxidative stress); alpha-1- acid glycoprotein - AGP and high-sensitivity C-reactive protein – CRP-hs (inflammation); leukocytes, neutrophils, lymphocytes and monocytes (immune response); creatine kinase - CK and lactate dehydrogenase - LDH (muscle injury); and exhaustion test, anaerobic threshold and aerobic capacity (performance). Additionally we assessed food intake and body composition; training loads in the season; psychometric behavioral and sleep; cardiac autonomic modulation; liver and kidney enzymes; glucose and lipid profile; plasma nitrite. The statistical analysis showed that GJG increased in total antioxidant capacity (22.5±5.5 vs. 31.2±12.3; p= 0.009), vitamin A (35.5±3.2 vs 39.7±4.0; p= 0.010) and uric acid (3.9±1.6 vs. 5.0±1.1; p= 0.005), when compared the pre and post intervention moments. MDA and vitamin E the values remained unchanged in both groups. The inflammatory marker AGP significantly decreased in GJG (77.2±17.5 vs 64.2±16.8; p= 0.006), but the same not occurred in the CG (64.9±15.8 vs 62.8±19.5; p = 0.480). The run time to exhaustion significantly improved in GJG in 15.3% (89.1±49.9 vs 101.9±56.0; p= 0.002), while GC showed a slight decrease of 2.2% (69.0±34.0 vs 68.2±33.2; p= 0.880). The anaerobic threshold increased 3.6% to GJG (10.6±2.3 vs 11.0±2.4; p= 0.510), while CG decreased 1.6% (11.8±2.1 vs 11.6 ± 2.8; p= 0.820), both descriptively, no significant changes. For aerobic capacity both groups showed discrete increases, but not significant (PGJ: 2.2% vs GC: 2.3%). Immunocompetence and injure muscle markers remained unchanged in both groups. Based on the results we can conclude that the intake of 10 ml/ kg/ day of integral purple grape juice for 28 days have ergogenic capacity to promote improved physical performance, accompanied by increase in antioxidant activity and possible reduced systemic inflammation. / O uso de suplementos esportivos já é algo bem estabelecido na prática desportiva, entretanto os anos recentes têm sido marcados pela descoberta de alimentos que desempenham atividade ergogênica similar à dos suplementos esportivos. Vários destes alimentos mostraram-se capazes de minimizar estresse oxidativo, inflamação e dano muscular induzidos por treinamentos intensos. As uvas tintas e seus derivados têm uma particular ação antioxidante e anti-inflamatória devido a sua rica composição em polifenóis, de modo que tem sido demonstrada capacidade substancial de melhorar parâmetros cardiometabólicos em hipertensos, diabéticos, nas doenças neurodegenerativas e hepáticas. Mas até o momento são poucos os estudos que atestaram a capacidade ergogênica do suco de uva em atletas. Neste contexto o presente estudo objetivou investigar os efeitos da suplementação de suco de uva tinto integral sobre estresse oxidativo, inflamação, imunocompetência, desgaste muscular e desempenho em corredores recreacionais. Para tanto um estudo experimental, randomizado e controlado foi conduzido com 28 corredores recreacionais. Foram configurados dois grupos: suco de uva (GSU; n=15, 42,7±8,1 anos) que ingeriu 10 mL/kg/dia de suco de uva tinto integral e grupo controle (GC; n=13, 36,3±8,0 anos) que consumiu bebida de carboidrato isocalórica, isoglicídica e isovolumétrica diariamente, durante 28 dias. Os parâmetros avaliados foram capacidade antioxidante total - CAT, vitaminas A e E, ácido úrico e malondialdeído-MDA (estresse oxidativo); alfa-1-glicoproteína ácida - AGP e proteína-C reativa ultrassensível - PCR-us (inflamação); leucócitos, neutrófilos, linfócitos e monócitos (imunocompetência); creatinaquinase - CK e lactato desidrogenase - LDH (desgaste muscular); e teste de exaustão, limiar anaeróbio e capacidade aeróbia (desempenho). Adicionalmente avaliou-se consumo alimentar e composição corporal; cargas de treino na temporada; comportamento psicométrico e sono; modulação autonômica cardíaca; enzimas hepáticas e renais; perfil lipídico e glicêmico; nitrito plasmático. A análise estatística mostrou que GSU aumentou significativamente a capacidade antioxidante total (22,5±5,5 vs 31,2±12,3; p=0,009), vitamina A (35,5±3,2 vs 39,7±4,0; p=0,010) e ácido úrico (3,9±1,6 vs 5,0±1,1; p=0,005) quando comparados os momentos pré e pós-intervenção, enquanto o GC não apresentou nenhuma diferença. Para MDA e vitamina E os valores permaneceram inalterados nos dois grupos. O marcador inflamatório AGP reduziu significativamente no GSU (77,2±17,5 vs 64,2±16,8; p=0,006), mas o mesmo não aconteceu no GC (64,9±15,8 vs 62,8±19,5; p=0,480). O tempo de corrida até a exaustão melhorou significativamente no GSU em 15,3 % (89,1±49,9 vs 101,9±56,0; p=0,002), enquanto GC apresentou uma redução descritiva de 2,2% (69,0±34,0 vs 68,2±33,2; p=0,880). O limiar anaeróbio aumentou em 3,6% para GSU (10,6±2,3 vs 11,0±2,4; p=0,510), enquanto GC reduziu 1,6% (11,8±2,1 vs 11,6±2,8; p=0,820), ambos descritivamente, sem diferença significativa. Para a capacidade aeróbia os dois grupos mostraram aumentos discretos, porém não significativos (GSU: 2,2% vs GC: 2,3%). Marcadores de imunocompetência e desgaste muscular mantiveram-se inalterados para os dois grupos. Com base nos resultados podemos concluir que a ingestão de 10mL/kg/dia de suco de uva tinto integral durante 28 dias tem capacidade ergogênica por promover melhoria do desempenho físico, acompanhada de aumento na atividade antioxidante e, possível, redução a inflamação sistêmica.

Em função do número reduzido de estudos sobre o movimento da água e de íons no bulbo molhado, foi realizado este trabalho com o objetivo de avaliar a distribuição de água e concentração de nutrientes no bulbo formado pelo sistema de gotejamento enterrado em comparação ao superficial. O ensaio foi conduzido na área experimental do Departamento de Engenharia Rural da Escola Superior de Agricultura "Luiz de Queiroz" - ESALQ/USP, localizada no município de Piracicaba, SP. Para a avaliação foram abertas trincheiras e instaladas sondas de TDR, para o monitoramento da distribuição da solução, dispostas a 0,5; 0,15; 0,25; 0,35 e 0,45 m - profundidade, e a 0,5; 0,15; 0,25; 0,35 m - comprimento, totalizando 17 sondas em malha por trincheira. A solução de KNO3 foi aplicada a partir de gotejadores com diferentes vazões (2 e 4 L/h), sendo aplicado o volume de 1 L em intervalos de 1 hora, totalizando 10 L de solução por repetição, Conjuntamente ao monitoramento da distribuição da solução no solo, medições da área superficial molhada e do disco saturado foram feitas com régua milimetrada, além disso, foi estabelecido um volume de controle onde foram avaliadas a uniformidade de aplicação de água e KNO3. Desta forma, foi possível verificar que: em relação aos sistemas superficiais, os sistemas enterrados apresentaram menor área superficial molhada e atingiram uma maior largura e profundidade; a umidade inicial do solo influencia a formação do bulbo molhado e o movimento do KNO3 no solo; a uniformidade de sais distribuídos no volume de controle é afetada negativamente pelo aumento do volume aplicado e as maiores concentrações de água e concentração de KNO3 foram obtidas próximas ao ponto de emissão. / In function of the reduced number of studies on the water and ions movement in the wet soil volume, this work was conducted with the goal of evaluating the distribution of water and nutrient concentration in the wetted soil volume formed by subsurface drip irrigation (SDI) in comparison to surface system. The rehearsal was concducted in the experimental area of the Rural Engineering Department - ESALQ/USP, located in Piracicaba, SP. Trenches were opened and installed probes of TDR for studying to solution distribution, to facilitate the study a mesh of probes was disposed to 0,5; 0,15; 0,25; 0,35 and 0,45 m - depth, and to 0,5; 0,15; 0,25; 0,35 m - length, with the total of 17 probes. The solution of KNO3 was applied starting from emitters with different flows (2 and 4 L/h), it was applied the volume of 1 L within intervals of 1 hour, with the total of 10 L of solution for repetition. All together to study the solution distribution in the soil, the wet superficial area measurements and of the saturated disc they were done by the ruler, besides, it was established a control volume where the uniformity application of water and KNO3 was analysed. This way, it was possible to verify that: in relation to the superface systems, the SDI presented smaller wet superficial area and they reached a larger width and depth; the initial soil water content influences the formation of the wetted soil volume and the KNO3 movement in the soil; the salt uniformity, distributed in the control volume, is affected negatively by the applied volume increased, and the largest concentrations of water and KNO3 were obtained close to the emission point.

O objetivo deste trabalho foi enriquecer consórcio microbiano a partir de mistura de lodo granular de digestor anaeróbio de fluxo ascendente sob condições fototróficas anoxigênicas. Por meio de técnica de biologia molecular foi possível identificar 17 unidades taxonômicas operacionais (UTO) no consórcio microbiano, dentre as quais seqüências similares a Rhodobacter, gênero amplamente citado nos estudos de produção de gás hidrogênio por bactérias fototróficas. Exames microscópicos do consórcio fototrófico indicaram predomínio de bacilos Gram-negativos. Ensaios sob condições fototróficas foram realizados com dois meios de cultivo (RCVB e FANG) e os seguintes substratos orgânicos: ácido acético, butírico, cítrico, lático e málico, empregados como fonte de carbono, tanto para o crescimento celular, como para a produção do gás hidrogênio. A relação C/N inicial foi 30/4 e posteriormente 15/2, com o objetivo de favorecer o crescimento celular e a produção do \'H IND.2\'. A concentração dos substratos foi determinada de forma com que essa relação se mantivesse a mesma. O crescimento celular e consumo dos ácidos orgânicos foram similares para os dois meios de cultivo empregados. Entretanto, a produção do gás hidrogênio foi maior nos ensaios com o meio FANG. Dentre os substratos utilizados o consumo dos ácidos cítrico e málico foram os maiores (~100%), para concentrações iniciais de 3,3 g/L e 2,6 g/L, respectivamente. O menor consumo 25% foi observado em meio RCVB e ácido acético (2,5 g/L). O crescimento da biomassa variou de 0,06 g/L a 1,1 g/L, enquanto que a velocidade máxima específica de crescimento variou de 0,4 a 0,2 g SSV/L.d entre os substratos utilizados. A menor e maior concentração de hidrogênio foram de 8,5 e 22 mmol \'H IND.2\'/L, para os reatores alimentados com ácido lático e málico em meio FANG, respectivamente. Pôde-se concluir que o consórcio fototrófico enriquecido foi capaz de utilizar os ácidos orgânicos para produção do gás hidrogênio. / The aim of this work was enrich a mixture of granular sludge of an up flow anaerobic sludge blanket (UASB) under anoxygenic phototrophic conditions. The techniques of molecular biology identified 17 operational taxonomic units (UTO) in the microbial consortium among the sequences analised, which were similar to Rhodobacter, genus widely cited in studies of hydrogen gas production by phototrophic bacteria. Microscopic examinations of the phototrophic consortium showed predominance of Gram-negative bacilli. Tests were conducted under phototrophic conditions with two culture media (RCVB and FANG) and the following organic substrates: acetic, butyric, citric, lactic and malic acids that were used as carbon source for both cell growth and for the hydrogen gas production. The carbon nitrogen ratio (C/N) in the preliminaries tests was 30/4 and then it was changed to15/2 in order to improve the cell growth and hydrogen production. The concentration of substrates was determined for remain the same carbon/nitrogen ratio among the substrates. The cell growth and consumption of organic acids were similar for the two culture media used. However, the production of hydrogen gas was higher in trials with the medium FANG. Among the substrates used, the consumption of malic and citric acids were the highest (~100%) for initial concentrations of 3.3 g/L and 2.6 g/L, respectively. The shortest consumption (25%) was observed for the cells that grew on acetic acid, 2.5 g/L in RCVB culture medium. The growth of the biomass varied from 0.06 g/L to 1.1 g/L, whereas the maximum specific growth rate ranged from 0.4 to 0.2 g VSS/L.d between the substrates used. The lowest and highest concentrations of hydrogen were 8.5 and 22 mmol \'H IND.2\'/L for the reactor fed with lactic acid and malic acid in FANG\'s medium, respectively. It was concluded that the phototrophic consortium was able to use those organic acids for the production of hydrogen gas.

As bactérias fototróficas freqüentemente apresentam florescimentos em lagoas de estabilização utilizadas no tratamento de esgoto sanitário, formando uma camada de cor púrpura na sua superfície. Portanto, o estudo das condições que propiciam tais florescimentos, a diversidade microbiana, o potencial de remoção da matéria orgânica e o estabelecimento das relações entre tais conhecimentos, permitem compreender o metabolismo do sistema. Nesse sentido, o objetivo deste trabalho foi avaliar a diversidade de bactérias (domínio Bacteria), bactérias fototróficas púrpuras e bactérias redutoras de sulfato (BRS) em lagoas de estabilização do Vale do Ribeira (Cajati, SP). Para tal, foram realizadas coletas sazonais (primavera, verão, outono e inverno) na sub-superfície, camada intermediária e interface água-sedimento, em dois horários (14:00 h e 02:00 h), nas lagoas anaeróbia e facultativa. Para analisar os diferentes grupos de microrganismos, utilizou-se a técnica de PCR/DGGE, com primers específicos. Nas análises de filogenia realizou-se o seqüenciamento parcial do gene RNAr 16S e da subunidade M do centro de reação fotossintético das bactérias fototróficas púrpuras. Análises físico-químicas, tais como sulfato, DQO, sólidos, nitrogênio e fósforo foram realizadas, além da determinação da concentração de oxigênio dissolvido, pH, temperatura e radiação solar fotossinteticamente ativa incidente. No outono observou-se maior diversidade de microrganismos do domínio Bacteria, bactérias fototróficas púrpuras e BRS, enquanto na primavera foi verificada a menor diversidade desses microrganismos para as duas lagoas. Na lagoa facultativa foi observada maior diversidade do domínio Bacteria e das BRS em relação à lagoa anaeróbia. Verificou-se maior diversidade de bactérias fototróficas púrpuras na lagoa anaeróbia, caracterizada por duas populações predominantes nas quatro estações e nas diferentes profundidades. A concentração de matéria orgânica (DQO) variou de 60,3 mg/L (inverno) a 298,0 mg/L (primavera) e a maior concentração de sulfato observada foi de 51,0 mg/L (inverno). Bacilo curvo Gram negativo, semelhante à bactéria fototrófica púrpura não sulfurosa, presente em amostra proveniente da sub-superfície da lagoa anaeróbia foi purificado e apresentou 92% de similaridade com Rhodopseudomonas palustris. Em ambas as lagoas foram identificadas bactérias semelhantes a Chromobacterium suttsuga (95%), Clostridium sp. (99%), Rhodobacter sphaeroides (99%), Rhodopseudomonas palustris (99%), Lampropedia hyalina (97%), Campylobacter fetus (99%), Desulfovibrio vulgaris (95%), Rhodospirillum rubrum (95%) e diferentes bactérias não cultivadas. / The phototrophic bacteria frequently blossom in the stabilization lagoons that are used in sanitary sewer treatment, forming a purple layer on its surface. Therefore, the study of the conditions that propitiate such blooms, the microbial diversity, the removal of the organic matter and the establishment of the relations between them permit to understand the metabolism of the system. The objective of this work was to evaluate the diversity of the bacteria (Bacteria domain), purple phototrophic bacteria and sulfate reducing bacteria (SRB) in stabilization lagoons of Vale do Ribeira (Cajati - SP). For this, it was made seasonal collects (spring, summer, autumn and winter) from the sub-surface, intermediate layer and interface water-sediment, at two times (14:00 h and 02:00 h) of the anaerobic and facultative lagoons. To analyze the different groups of microorganisms it was used the PCR/DGGE technique, with specific primers; for the phylogenic analysis it was realized the DNA partial sequencing of the 16S RNAr gene and of the subunit M of the photosynthetic center of reaction of the purple photosynthetic bacteria. It was determined: the concentration of dissolved oxygen, pH, temperature and photosynthetically active incident solar radiation, and the physical-chemistry analysis as: COD, solids, nitrogen and phosphorus. In the autumn it was observed greater diversity of microorganisms of the Bacteria domain, the group of the purples phototrophic bacteria and SRB, while in the spring it was verified minor diversity of these microorganisms in the two lagoons studied. In the facultative lagoon it was observed greater diversity of the Bacteria domain and of the SRB with respect to the anaerobic lagoon. It was verified greater diversity of the purple phototrophic bacteria in the anaerobic lagoon, of what in the facultative lagoon, which was characterized by the two predominant populations in the four seasons and in the different points of collect. The concentration of the organic matter (COD) varied from 60,3 mg/L (winter) to 298,0 mg/L (spring) and the greater concentration of sulfate observed was of 51,0 mg/L (winter). Arched bacillus Gram-negative similar to purple not sulfurous bacteria, from a sample of the sub-surface of the anaerobic lagoon was purified and presented 92% of similarity with Rhodopseudomonas palustris. In both lagoons it was identified bacteria similar to Chromobacterium suttsuga (95%), Clostridium sp. (99%), Rhodobacter sphaeroides (99%), Rhodopseudomonas palustris (99%), Lampropedia hyalina (97%), Campylobacter fetus (99%), Desulfovibrio vulgaris (95%), Rhodospirillum rubrum (95%).