Apesar do excelente prognóstico dos tumores de Wilms (TW) localizados (estádios I e II) e de histologia favorável (HF), 10% deles recidivam. Em 122 pacientes com TW com essas características, diagnosticados de 1976 e 2001, analisamos alguns fatores clínicos, como a idade por ocasião do diagnóstico e peso do tumor, em todos os pacientes; fatores biológicos, como o TP53 e a glicoproteína-p, em 40 deles; e variáveis histológicas de microestadiamento (invasão de seio renal, cápsula tumoral, vasos intra-renais e pseudocápsula inflamatória) em 28 com TW em estádio I. Correlacionando todos esses fatores com a presença de recidiva, observamos que a chance maior de recidiva estatisticamente significativa somente foi verificada em pacientes com duas ou mais variáveis de microestadiamento e/ou peso tumoral maior que 550 g / In spite of the excellent prognosis of localized favorable histology (FH) of Wilms' tumor (WT), 10% of them will relapse. In 122 TW patients with these characteristics, diagnosed between 1976 and 2001, some clinical factors have been analyzed, such as age at diagnosis and tumor weight in all patients; biological factors, like TP53 and p-glycoprotein, in 40 of them; and microsubstaging histological variables (invasion of renal sinus, tumor capsule, intrarenal vessels, and inflammatory pseudocapsule). Correlating all of those factors with relapse, we have observed that only patients with the association of two or more microsubstaging variables and/or tumor weight over 550 g showed a statistically significant higher chance of relapse

Introdução: A papilomatose recorrente respiratória (PRR) é uma doença benigna de enorme morbidade, com manifestações clínicas muito distintas, causada pelo papiloma vírus humano (HPV) dos tipos 6 e 11. Objetivo: Identificar os diferentes tipos de HPV e suas variantes relacionados com a PRR e estabelecer possível correlação clínica entre o tipo / variante viral e a agressividade da doença. Casuística e Métodos: Estudo prospectivo, realizado com 41 pacientes atendidos no Ambulatório de Laringologia do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo, entre os anos de 2008 e 2015, que apresentaram quadro de PRR. Foi realizada genotipagem viral das biopsias laríngeas e os vírus presentes foram relacionados com a evolução clínica de cada paciente e a agressividade da doença, pelo escore de Derkay, número de cirurgias e traqueostomia. Resultados: Dos 41 pacientes avaliados, a maioria era do sexo masculino (68,29%), e maiores de 12 anos (63,41%). Os menores de 12 anos apresentaram número de cirurgias e escore de Derkay significativamente maiores do que os pacientes mais velhos. Foram identificados somente os HPV dos tipos 6 e 11, na frequência de 73,17% e 26,83%, respectivamente. A agressividade da doença não mostrou relação com o tipo ou a variante do vírus, mas sim com a idade de apresentação clínica da doença. Conclusão: Os tipos de HPV e as suas variantes não apresentaram comportamento clínico mais agressivo na PRR no grupo estudado. Em contrapartida, a idade foi determinante para a agressividade da doença. / Introduction: Recurrent respiratory papillomatosis (RRP) is a benign disease of enormous morbidity that presents very distincts clinical manifestations. It is usually caused by the human papillomavirus (HPV), mostly types 6 and 11. Purpose: To identify the different HPV types and variants related to RRP and to establish a possible correlation between HPV type/variant and viral disease aggressiveness. Casuistic and Methods: This prospective study included 41 patients followed at the Outpatient Clinic of Laryngology of the Medical School of Ribeirão Preto, University of São Paulo, between 2008 to 2015, due to RRP. Virus genotyping was performed on laryngeal biopsies by PCR, and this information was associated to each patient\'s data regarding aggressiveness of the disease and clinical evolution, by Derkay\'s score, number of surgeries and tracheostomy. Results: Most patients were male (68.29%) and older than 12 years-old (63.41%). Patients younger than 12 years had a significantly higher number of surgeries and Derkay score than older patients. In all samples, only HPV 6 and HPV 11 were detected, in a prevalence of 73.17% and 26.83%, respectively. The aggressiveness of the disease was not related to the type or variant of the virus, but to the age of patient when the clinical presentation of the disease occurred. Conclusions: HPV types and variant of HPV showed no more aggressive clinical behavior in recurrent respiratory papillomatosis in the studied group. In contrast, age was crucial to the aggressiveness of the disease.

O objetivo deste trabalho é provar um Closing Lema Parcial para variedades bidimensionais compactas, orientáveis ou não--orientáveis. Para enunciá--lo, considere um campo vetorial \\linebreak $X\\in\\mathfrak^r(M)$, $r\\ge 2$, de classe $C^r$ em uma variedade bidimensional compacta $M$, e seja $\\Sigma$ um segmento transversal a $X$ passando por um ponto recorrente não--trivial $p$ de $X$. Seja $P:\\Sigma\\to\\Sigma$ a correspondente transformação de primeiro retorno. O primeiro resultado deste trabalho consiste em mostrar que se $P$ tem a propriedade de que para todo $n\\ge N$ e $x\\in{m dom}\\,(P^n)$, $\\vert DP^n(x)\\vert<\\lambda$, onde $N\\in\\N$ e $0<\\lambda<1$, então existe um campo vetorial $Y$ arbitrariamente próximo de $X$ na topologia $C^r$ tendo uma trajetória periódica passando por $p$. O segundo resultado consiste em apresentar condições, sobre os expoentes de Lyapunov de $P$, para que $\\vert DP^n\\vert<\\lambda$ para todo $n\\ge N$. Nesta tese, também incluímos um resultado sobre a estabilidade assintótica no infinito de campos planares diferenciáveis, mas não necessariamente de classe $C^1$. / The aim of this work is to provide a Partial $C^r$ Closing Lemma for compact surfaces, orientable or non--orientable. To state it, let $X\\in\\mathfrak^r(M)$, $r\\ge 2$, be a $C^r$ vector field on a compact surface $M$ and let $\\Sigma$ be a transverse segment to $X$ passing through a non--trivial recurrent point $p$ of $X$. Let $P:\\Sigma\\to\\Sigma$ be the corresponding first return map. The first result of this work consists in showing that if $P^n$ has the property that for all $n\\ge N$ and $x\\in{m dom}\\,(P^n)$, $\\vert DP^n(x)\\vert<\\lambda$, where $N\\in\\N$ e $0<\\lambda<1$, then there exists a vector field $Y$ arbitrarily close to $X$ in the $C^r$ topology such that $p$ is a periodic point of $Y$. The second result consists in presenting sufficient conditions, upon the Lyapunov exponents of $P$, so that $\\vert DP^n\\vert<\\lambda$ for all $n\\ge N$. In this thesis, we also include a result concerning the asymptotic stability at infinity of planar differentiable vector fields, not necessarily of class $C^1$.

Um dos desafios recorrentes na prática clínica da Oncologia é o processo de repopulação, no qual células tumorais resistentes à terapia são capazes de proliferar e reconstituir o tumor. No entanto, os mecanismos envolvidos neste fenômeno ainda foram pouco explorados, sendo necessário melhor compreendê-los para evitar a falha terapêutica. Melanomas são bons modelos para estudar repopulação devido às baixas taxas de sobrevida livre de progressão e às altas taxas de resistência às terapias associadas a este tipo de tumor sólido. Sabe-se que a exposição de células tumorais a condições estressoras do microambiente, como hipóxia e hipóxia/reoxigenação, bem como ao próprio tratamento antitumoral, são pressões seletivas frequentemente encontradas em tumores sólidos que favorecem a resistência às terapias e a repopulação tumoral. Estudos prévios indicam que a sinalização mediada pelo Fator de Ativação de Plaquetas (PAF), um lipídio bioativo relacionado à diversas funções fisiológicas, e de seu receptor, PAFR, está associada com a resistência de células de melanoma aos tratamentos citotóxicos e com crescimento tumoral. Portanto, este trabalho teve como objetivo investigar o envolvimento da sinalização de PAFR frente às condições estressoras descritas acima no fenômeno de repopulação de melanomas. Os resultados de espectrometria de massa indicaram que hipóxia aumentou a geração de PAF nas linhagens de melanoma humano SKmel05 e A375, mas não na A375M, embora este aumento não tenha sido observado após a reoxigenação. Além disso, mostraram que SKmel37 exibiu os maiores níveis basais de PAF, aumentando substancialmente sua geração em diferentes tempos de exposição à hipóxia e hipóxia/reoxigenação. Investigamos também a geração de outros ligantes de PAFR, porém nenhum deles foi encontrado nas amostras. Os resultados de detecção de PAFR por Western Blot e/ou citometria de fluxo revelaram que os níveis proteicos não foram modulados por estas condições em nenhuma das linhagens e que, em termos basais, SKmel37 e SKmel05 apresentaram os maiores níveis. Estas linhagens foram, portanto, submetidas a ensaios de proliferação, os quais evidenciaram que frente ao tratamento com o antagonista de PAFR, WEB2086, em condições de hipóxia e hipóxia/reoxigenação, apenas as células SKmel37 tiveram sua proliferação reduzida e morfologia associada à morte. Ensaios de incorporação de iodeto de propídio indicaram que o tratamento destas células expostas à hipóxia/reoxigenação com WEB2086 levou a acúmulo em SG2M, morte celular e sensibilização à cisplatina. Além disso, imunofluorescência de cortes congelados de tumores induzidos com SKmel37 revelou que houve acúmulo de PAFR em áreas hipóxicas e em seu entorno. Em relação ao modelo de exposição à tratamentos antitumorais, por sua vez, curvas de concentração e tempo com Vemurafenib mostraram que SKmel37 e A375 foram resistentes à droga, ao passo que SKmel05 e UACC62 foram sensíveis. Além disso, WEB2086 potencializou o efeito de morte induzido por Vemurafenib nas linhagens sensíveis, mas não afetou as resistentes. Considerando os aspectos clínicos de resposta inicial com posterior desencadeamento de repopulação, seguimos com as linhagens sensíveis e verificamos por citometria que esta droga aumentou ROS em ambas as linhagens, mas só aumentou PAFR extracelular na SKmel05. O tratamento combinado potencializou a geração de ROS e levou a ativação de caspase3/7 apenas na SKmel05. Esta linhagem foi então submetida a ensaios clonogênicos cujos resultados mostraram que o tratamento com Vemurafenib reduziu o número de clones e que WEB2086 não potencializou este efeito. Assim, o conjunto de resultados apresentados evidencia que a sinalização de PAFR participa dos desfechos de sobrevivência frente à hipóxia/reoxigenação e/ou tratamentos antitumorais, podendo, de alguma forma, contribuir com a repopulação de melanomas / One of the recurrent challenges in the clinical practice of Oncology is the process of repopulation, in which therapy-resistant tumor cells can proliferate and reconstitute the tumor. However, the mechanisms involved in this phenomenon were still little explored. The understanding of these events is, therefore, needed to avoid therapeutic failure. Melanomas are good models for studying repopulation due to the low rates of progression-free survival and the high rates of resistance to therapies associated to this type of solid tumor. It is known that the exposure of tumor cells to microenvironmental stress conditions, such as hypoxia and hypoxia/reoxygenation, as well as the exposure to antitumor treatment itself, are selective pressures frequently found in solid tumors that favor therapy resistance and tumor repopulation. Previous studies have indicated that the signaling mediated by the Platelet Activation Factor (PAF), a bioactive lipid related to various physiological functions, and its receptor, PAFR, is associated with resistance of melanoma cells to cytotoxic treatments and with tumor growth. Therefore, the aim of this study was to investigate the involvement of PAFR signaling upon the adverse conditions described above in the phenomenon of melanoma repopulation. Mass spectrometry results indicated that hypoxia increased the generation of PAF in human melanoma cell lines SKmel05 and A375, but not in A375M, although this increase was not observed after reoxygenation. In addition, they showed that SKmel37 exhibited the highest PAF basal levels, whose generation increased substantially after different times of hypoxia and hypoxia/reoxygenation exposure. We also investigated the generation of other PAFR ligands, but none were found in the samples. The results of PAFR detection by Western Blot and/or flow cytometry revealed that protein levels were not modulated by these conditions in any of the cell lines and that, at baseline, SKmel37 and SKmel05 showed the highest levels. These lines were therefore submitted to proliferation assays, which showed that the treatment with the PAFR antagonist, WEB2086, under conditions of hypoxia and hypoxia/reoxygenation, led to proliferation reduction and death-associated morphology in SKmel37 cells only. Propidium iodide incorporation studies indicated that the treatment of these cells exposed to hypoxia/reoxygenation with WEB2086 led to accumulation in SG2M, cell death and cisplatin sensitization. In addition, immunofluorescence of frozen sections of SKmel37-induced tumors revealed that PAFR was found accumulated in hypoxic areas and its surroundings. Regarding the model of exposure to antitumor treatments, concentration and time curves with Vemurafenib showed that SKmel37 and A375 were resistant to the drug, whereas SKmel05 and UACC62 were sensitive. In addition, WEB2086 potentiated the effect of Vemurafenib-induced death on sensitive cell lines but did not affect the resistant ones. Considering the clinical aspects of initial response with subsequent repopulation triggering, we continued using the sensitive cell lines and we verified by cytometry that this drug increased ROS in both cell lines but only increased extracellular PAFR in SKmel05. The combined treatment potentiated the generation of ROS and led to the activation of caspase3/7 in SKmel05 only. This cell line was then submitted to clonogenic assays whose results showed that treatment with Vemurafenib reduced the number of clones and that WEB2086 did not potentiate this effect. Thus, the set of results presented highlights that PAFR signaling participates in the survival outcomes upon hypoxia/reoxygenation and/or antitumor treatments, and may, in some way, contribute to the repopulation of mel

Le cancer de prostate (CaP) est le plus fréquent chez l’homme de plus de 50 ans. La généralisation du PSA et du dépistage du CaP a permis son diagnostic à des formes localisées (80% des formes diagnostiquées), accessibles à un traitement curatif. De plus, un certain nombre de ces formes localisées sont indolentes, de très faible risque, éligibles à une surveillance active (SA).Cependant, 15 à 25% des patients vont présenter une récidive 10 ans après un traitement curatif, et environ 30% des patients inclus en SA seraient en fait sous-évalués (cancer significatif). Il est donc nécessaire d’identifier de nouveaux biomarqueurs pronostiques capables de distinguer les tumeurs indolentes des tumeurs à haut risque, et capables d’améliorer la prédiction du risque de récidive après traitement curatif. Objectifs : Evaluer la valeur pronostique potentielle du remodelage de la signalisation calcique dans le cancer de prostate : 1) Evaluer le lien entre remodelage de la signalisation calcique et agressivité tumorale 2) Evaluer l’impact pronostique du remodelage de la signalisation calcique sur la récidive systémique après prostatectomie radicale(PR). 3) Evaluer l’apport du remodelage de la signalisation calcique dans la sélection des patients éligibles à la surveillance active. Méthodologie 1) Evaluation de l’expression (immunohistochimie) de TRPC1, TRPC4, Orai1 et STIM1 à différents stades de progression du CaP, et évaluation de l’impact pronostique de cette expression sur la récidive dans le CaP localisé à partir d’une cohorte de 238 patients. 2) Etude cas-témoin à partir d’une cohorte de 112 patients opérés d’un CaP localisé. Evaluation de l’impact pronostique de l’expression (immunohistochimie) de 10 acteurs de la signalisation calcique (Orai1, Orai2, Orai3, STIM1, STIM2, TRPV5 et V6, TRPC1 et C4, TRPM8) sur la récidive systémique après PR. 3). A partir d’une cohorte de patients ayant été opérés pour des cancers de faible risque, ont été inclus les patients potentiellement éligibles à une SA au moment du diagnostic (n=71). Etude de l’apport de la signalisation calcique dans la sélection des patients éligibles à la SA (marquage immunohistochimie sur biopsies et pièce opératoire : TRPC1 et C4, TRPV5 et V6, Orai1, STIM1) Résultats 1) Dans le Cap localisé, une surexpression de TRPC1 était associée à une prolifération plus faible et une meilleure survie sans récidive (indépendant des marqueurs pronostiques usuels). 2) Une surexpression de TRPC4, TRPV5 et TRPV6 est associée à un plus faible risque de récidive systémique après prostatectomie, et ce indépendamment des 5 facteurs pronostiques usuels. 3) Un marquage plus intense de TRPV6 sur les biopsies prostatiques semble associé à un CaP significatif, éligible à un traitement curatif d’emblée chez les patients présentant un CaP de faible risque. Conclusion : Sur pièce de PR, TRPC1, TRPC4, TRPV5 et TRPV6 possèdent une valeur pronostique indépendante et sont associés à un plus faible risque de récidive après PR. Sur biopsie prostatique, TRPV6 permet de distinguer les CaP indolents des significatifs chez les patients présentant un CaP de faible risque. / Prostate cancer (PCa) is the most common malignancy in men 50 years and older and the second leading cause of cancerrelated death in men in developed countries. Widespread PSA screening has allowed an increase rate of localised PCa at diagnosis, managed by curative treatment such as radical prostatectomy (RP), or active surveillance (AS) in case of indolent disease. However, 30% of patients experience biochemical relapse during the 10 years following RP. Moreover, 30% of patients in AS are undervalued and present a significant disease. It is therefore necessary to identify new prognostic biomarkers capable of distinguishing indolent from significant tumours, and capable to accurately predict the risk of recurrence after curative treatment. Objectives: To evaluate the potential prognostic value of calcium signalling remodeling in PCa: 1) To assess the impact of calcium signalling remodelling on tumour aggressiveness 2) To assess the prognostic value of calcium signalling remodelling on systemic recurrence after RP. 3) To assess the contribution of calcium signaling remodelling in the selection of patients for active surveillance (AS). Methods 1) Study of the expression (immunohistochemistry) of TRPC1, TRPC4, Orai1 and STIM1 at different stages of PCa, and assessment of the prognostic value of this expression on recurrence in clinically localised PCa (CLC) in a cohort of 238 patients. 2) Case-control study on a cohort of 112 patients who underwent RP for CLC. Evaluation of the prognostic impact of the expression (immunohistochemistry) of 10 actors of calcium signalling (Orai1, Orai2, Orai3, STIM1, STIM2, TRPV5 and V6, TRPC1 and C4, TRPM8) on systemic recurrence after RP. 3) From a cohort of patients who underwent RP for low-risk PCa, were enrolled patients potentially eligible for AS at the time of diagnosis (n = 71). We evaluated the impact of calcium signalling remodelling in patients selection for AS (immunohistochemical staining on biopsies and prostate specimens: TRPC1 and C4, TRPV5 and V6, Orai1, STIM1).Results 1) In CLC, an overexpression of TRPC1 was associated with a decreased proliferation, and with a higher rate of biochemical progression-free survival (independent of usual prognostic markers). 2) Overexpression of TRPC4, TRPV5 and TRPV6 was associated with a lower risk of systemic recurrence after RP, independently of the prognostic factors currently used. 3) More intense staining of TRPV6 on biopsies was associated with a significant PCa, for which a curative treatment is required. Conclusion: On RP specimens, overexpression of TRPC1, TRPC4, TRPV5 and TRPV6 has an independent prognostic value and is associated with a lower risk of recurrence after RP. On prostate biopsies, TRPV6 allows to distinguish indolent from significant disease in patients with low-risk PCa.

No description available.

The human brain is highly sensitive to social information and so is our language production system: people adjust not just what they say but also how they say it in response to the social context. For instance, we are sensitive to the presence of others, and our interactional expectations and goals affect how we individually choose to talk about and refer to things. This thesis is an investigation of the social factors that might lead speakers to adapt linguistically to others. The question of linguistic adaptation is conceived and addressed at two levels: as lexical convergence (i.e., interlocutors coordinating their lexical choices with each other), and as spatial perspective taking in language use (i.e., speakers abandoning their self perspective in favour of another's when verbally locating objects in space). What motivated my research was two-fold. First, I aimed to contribute to the understanding of the interplay between the automatic cognitive accounts and the strategic social accounts of linguistic convergence. At the same time, I wanted to explore new analytical tools for the investigation of interpersonal coordination in conversation (cross-recurrence quantification analysis (CRQA)). Second, there are conflicting explanations as to why people often abandon their self spatial perspective when another person is present in the environment. I aimed to clarify this by bringing together insights from different research fields: spatial language production, spatial cognition, joint attention and joint action. A first set of experiments investigated the effects of speakers' deceptive goals on lexical convergence. Given the extensive evidence that one interlocutor's choices of words shapes another's during collaborative interaction, would we still observe this coordination of linguistic behaviour under conditions of no coordination of intents? In two novel interactive priming paradigms, half of the participants deceived their naïve partner in a detective game (Experiment 1) or a picture naming/matching task (Experiment 2-3) in order to jeopardise their partner's performance in resolving the crime or in a related memory task. Crucially, participants were primed by their partner with suitable-yet-unusual names for objects. I did not find any consistent evidence that deceiving led to a different degree of lexical convergence between deceivers and deceived than between truthful interlocutors. I then explored possibilities and challenges of the use of cross-recurrence quantification analysis (CRQA) (a new analytical tool borrowed from dynamical systems) for the study of lexical convergence in conversation. I applied CRQA in Experiment 4, where I focused on the strategic social accounts of linguistic convergence and investigated whether speakers' tendency to match their interlocutors' lexical choices depended on the social impression that they formed of each other in a previous interaction, and whether this tendency was further modulated by the interactional goal. I developed a novel two-stage paradigm: pairs of participants first experienced a collectivist or an individualistic co-player in an economic decision game (in reality, a pre-set computer programme) and then engaged in a discussion of a survival scenario (this time with the real other) divided in an open-ended vs. joint-goal driven part. I found no evidence that the social impression of their interlocutor affected speakers' degree of lexical convergence. Greater convergence was observed in the joint-goal dialogues, replicating previous findings at syntactic level. Experiments 5-7 left the interactive framework of the previous two sets of experiments and explored spatial perspective taking in a non-interactive language task. I investigated why the presence of a person in the environment can induce speakers to abandon their self perspective to locate objects: Do speakers adapt their spatial descriptions to the vantage point of the person out of intentionality-mediated simulation or of general attention-orienting mechanisms? In an online paradigm, participants located objects in photographs that sometimes contained a person or a plant in various positions with respect to the to-be-located object. Findings were consistent with the simulated intentional accounts and linked non-self spatial perspective in language to the apprehension of another person’s visual affordance. Experiments 8-9 investigated the role of shared experience on perspective taking in spatial language. Prior to any communicative and interactional demand, do speakers adapt their spatial descriptions to the presumed perspective of someone who is attending to the same environment at the same time as them? And is this tendency further affected by the number of co-attendees? I expanded the previous online paradigm and induced participants into thinking that someone else was doing the task at the same time as them. I found that shared experience reinforced self perspective (via shared perspective) rather than reinforcing non-self perspective (via unshared perspective). I did not find any crowd effect.

Diante da indicação do tratamento da Classe II subdivisão com extrações dentárias, existe a alternativa de extrair três ou quatro pré-molares. Estes protocolos atuam diferentemente na relação molar porque o tratamento com três extrações mantém a relação molar de Classe II em um dos lados e a de Classe I no outro e o tratamento com quatro extrações busca atingir uma relação molar de Classe I bilateral, corrigindo a relação molar de Classe II presente no início do tratamento. Considerando-se que o sucesso do tratamento ortodôntico está relacionado com a manutenção das alterações promovidas, em longo prazo, e que alguns autores questionam a estabilidade do término com relação molar de Classe II, julgou-se pertinente comparar a estabilidade dos resultados promovidos por estes dois protocolos. Para tanto, foram selecionados 162 pares de modelos de gesso de 52 pacientes Classe II subdivisão, correspondentes a três estágios distintos: pré-tratamento (T1), pós-tratamento (T2) e de observação pós-tratamento (T3), ou seja, confeccionados 6,9 anos, em média, após o término do tratamento. O grupo 1 foi composto pelos modelos de gesso de 24 pacientes tratados com três extrações e com idade média de 13,54 anos (9,50 a 21,06) no pré-tratamento, de 17,03 anos (14,42 a 25,11) no pós-tratamento e de 23,45 anos (18,33 a 29,87) no estágio de observação pós-tratamento e o grupo 2 compreendeu os modelos de gesso de 28 pacientes tratados com quatro extrações e com idade média de 13,33 anos (10,51 a 15,68) no pré-tratamento, de 16,31 anos (14,01 a 20,86) no pós-tratamento e de 23,70 anos (17,18 a 35,16) no estágio de observação pós-tratamento. Calculou-se os valores dos índices oclusais PAR e IPT para todos os pares de modelos de gesso e, a partir deles, calculou-se as alterações do tratamento e pós-tratamento. O teste t independente foi empregado para a comparação entre os grupos das idades e dos valores dos índices oclusais PAR e IPT nos três estágios de avaliação, dos tempos de tratamento e de observação pós-tratamento, bem como das alterações ocorridas durante o tratamento e no período pós-tratamento, dos percentuais de melhora e de recidiva. A avaliação da estabilidade foi realizada pela subtração dos valores dos índices PAR e IPT pós-tratamento dos valores do estágio de observação pós- tratamento (PAR3-PAR2 e IPT3-IPT2), sendo que quanto maior a diferença, maior foi a recidiva. Os resultados não apontaram diferenças entre os grupos em nenhuma das variáveis estudadas. Desta forma, verificou-se que a estabilidade dos resultados do tratamento da Classe II subdivisão realizado com três ou quatro extrações é semelhante. / If Class II subdivision treatment with extractions is the best treatment alternative, it can comprise 3- or 4- premolar extractions. The main difference between these two protocols is their accomplishment to the posttreatment molar relationship because the first achieve a Class II molar relationship in one side and a Class I in the other and the second attain a bilateral Class I molar relationship. Considering that long-term stability is an important item of treatment success evaluation and stability of Class II molar relationship that is kept unchanged in some protocols is considered to be suspicious by some authors, the purpose of this study was to compare the occlusal stability of Class II subdivision malocclusion treatment with 3- and 4- premolar extractions. The sample comprised 162 pairs of dental casts obtained at T1 (pretreatment), T2 (posttreatment) and T3 (long-term posttreatment), that match to a mean of 6.9 years after treatment conclusion of 52 Class II subdivision patients. Group 1 comprised the dental casts of 24 patients treated with 3 premolar extractions and with a mean age of 13.54 years (range, 9.50 to 21.06) at pretreatment, 17.03 years (range 14.42 to 25.11) at posttreatment and 23.45 years (range, 18.33 to 29.87) at long-term posttreatment and group 2 included 28 patients\' dental casts treated with four premolar extractions and with a mean age of 13.33 years (range, 10.51 to 15.68) at pretreatment, 16.31 years (range, 14.01 to 20.86) at posttreatment and 23.70 years (range, 17.18 to 35.16) at long-term posttreatment. The PAR and TPI occlusal indexes were calculated for all the dental casts and they were used to calculate the treatment and long-term posttreatment changes. T tests were used to compare the groups´ ages and the indexes values at T1, T2 and T3, the treatment time, the long-term posttreatment time, the amount of treatment and long-term posttreatment changes and the percentages of reduction and relapse. Stability evaluation was calculated by subtracting the posttreatment from the long-term posttreatment index values (PAR3-PAR2 and TPI3-TPI2). The greater the difference, the greater the relapse. There were not significant differences between the groups\' variables considered in this study and it was concluded that treatment of Class II subdivision malocclusion with 3- and 4- premolar extractions have a similar long-term posttreatment stability.

nÃo hà / Esta dissertaÃÃo efetuou uma anÃlise empÃrica, baseada nas infraÃÃes de trÃnsito registradas no cadastro de veÃculos tipo motocicletas e similares de atà 125cc, ocorridas no Estado do CearÃ, no perÃodo de 2010 a 2013. O objetivo foi avaliar as caracterÃsticas sociodemogrÃficas que contribuem para aumentar a ocorrÃncia de reincidÃncia das infraÃÃes de trÃnsito por motociclistas, de acordo com as variÃveis: sexo, idade e tempo de habilitaÃÃo, e; a taxa de motorizaÃÃo e sua implicaÃÃo no fluxo de trÃnsito nos municÃpios cearenses. Para tanto, utilizouse o modelo economÃtrico Logit, cuja base de dados foi fornecida pelo Departamento Estadual de TrÃnsito â DETRAN-CE e pelo Instituto de Pesquisa e EstratÃgia EconÃmica do Cearà - IPECE. O que se observou, no Estado do CearÃ, à que os resultados corroboram as evidÃncias da literatura internacional: os infratores de trÃnsito reincidentes sÃo, em sua maioria, jovens e do sexo masculino, o que indica a necessidade de polÃticas pÃblicas de prevenÃÃo, incluindo esses grupos especÃficos da populaÃÃo. AlÃm disso, a condiÃÃo de habilitaÃÃo indica que os motociclistas mais experientes, ou com mais tempo de habilitaÃÃo, sÃo os que tÃm maior probabilidade de reincidÃncia. Para as estimaÃÃes acerca da taxa de motorizaÃÃo, os resultados sugerem que os condutores que estÃo nos municÃpios com taxas de motorizaÃÃo superiores à taxa de referÃncia de 20% apresentam uma maior probabilidade de reincidirem nas infraÃÃes de trÃnsito. / This paper made an empirical analysis based on traffic violations recorded in type vehicle registration motorcycles and the like up to 125cc, occurred in the state of CearÃ, in the period 2010 to 2013. The objective was to assess sociodemographic characteristics that contribute to increase the occurrence of repeated traffic violations by motorcyclists, according to variables such as gender, age and time of qualification, and; motorization rate and its implication in traffic flow in Cearà municipalities. For this, we used the logit econometric model, whose database has been provided by the State Department of Motor Vehicles â DETRAN-CE and the Institute of Economic Research and Strategy of Cearà - IPECE. What was observed in the state of CearÃ, is that the results support the evidence of the international literature: the repeat traffic offenders are mostly young and male, which indicates the need for preventive policies, including these specific population groups. In addition, the driver's condition indicates that the more experienced riders, or longer clearance, are the ones who are more likely to recur. For the estimations about the motorization rate, the results suggest that drivers who are in municipalities with motorization rates above the 20% reference rate are more likely to re-offend in traffic violations.

INTRODUÇÃO: Os macrófagos associados aos tumores (MAT) sólidos estão relacionados à progressão ou à involução das neoplasias, dependendo da diferenciação em M1 ou M2. No carcinoma basocelular (CBC), as formas mais agressivas apresentam aumento de macrófagos às custas do fenótipo M2, se comparadas às formas não invasivas. O tratamento do CBC sólido pela Cirurgia Micrográfica de Mohs (CMM) proporciona elevados índices de cura, porém recidivas podem ocorrer. OBJETIVOS: Comparar a população total de macrófagos e as subpopulações M1 e M2 nos casos de CBC sólidos recidivados e não recidivados após exérese pela CMM. METODOLOGIA: Cortes histológicos obtidos a partir dos blocos de parafina de nove casos de CBC sólidos recidivados após CMM e de 18 casos de CBC sólido operados pela CMM não recidivados foram marcados imunoistoquimicamente para iNOS, CD204, CD163 e CD68. A expressão desses marcadores foi analisada pelo método de análise de imagens. RESULTADOS: Não foram encontradas diferenças significativas entre os grupos em relação à porcentagem média de células M1 (INOS), células M2 (CD163 e CD204) e total de células (CD68). CONCLUSÃO: A recidiva dos tumores estudados não ocorreu por influência do MAT, mas pode ser decorrente da falha técnica na realização da CMM ou de algum outro mecanismo imunológico desconhecido / INTRODUCTION: The macrophages associated with solid tumors (MAT) are related to the progression or regression of tumors, depending on the differentiation in M1 or M2. In basal cell carcinoma (BCC), the most aggressive forms show an increase in macrophages at the expense of M2 phenotype compared to non-invasive forms. The treatment of BCC solid by Mohs micrographic surgery (MMS) provides high cure rates, but relapses can occur. OBJECTIVES: To compare the total population of macrophages and subpopulations M1 and M2 in cases of recurrent BCC solid and not recurrent after excision by MMS. METHODS: Histological sections obtained from paraffin blocks of 9 cases of recurrent solid CBC after MMS and 18 cases of solid CBC operated by MMS not relapsed were labeled immunohistochemically for iNOS, CD204, CD163 and CD68. The expression of these markers was analyzed by image analysis. RESULTS: No significant differences were found between the groups in relation to the average percentage of M1 cells (INOS), M2 cells (CD163 and CD204) and total cells (CD68). CONCLUSION: The recurrence of the tumors studied did not occur under the influence of MAT, but may be due to technical failure in achieving MMS or some other unknown immune mechanism