Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-05-18T18:09:32Z No. of bitstreams: 1 2015 - Bruno Oliveira de Carvalho.pdf: 1725115 bytes, checksum: 78e6b6f127705b066a8a0f75bbb08e88 (MD5) / Made available in DSpace on 2018-05-18T18:09:32Z (GMT). No. of bitstreams: 1 2015 - Bruno Oliveira de Carvalho.pdf: 1725115 bytes, checksum: 78e6b6f127705b066a8a0f75bbb08e88 (MD5) Previous issue date: 2015-03-17 / CAPES / The knowledge of bacterial genetic information has enable advances in many areas allowing detecting microorganisms and evaluating the production of virulence factors and resistance in certain populations. This knowledge allows the detection of microorganisms, as well as the evaluation of virulence and resistance factors production in certain populations. More recently, molecular diagnosis based on the detection of specific fragments from these agents has been implemented in no viable bacterial cultivation environments or hampered by biotic or abiotic conditions. Besides specific agent detection, bacterial diversity analysis might be an important criterion, such as assessing soil quality. Cultivation has been the most common way for evaluating bacterial diversity. However, most of soil bacteria are not cultivable; therefore, the employment of molecular tools has been increasingly used on this sort of analysis. This survey aimed standardizing the use of cultivation independent techniques for granting three agriculture sectors demands. The first chapter standardized the employment of DGGE technique for analyzing milk bacterial diversity from mastitis and healthy teats belonging to the same animal. Results pointed to differences on bacterial profile from both groups. The second chapter evaluated the impact of the employment of drill cuttings with castor beans and cramble pies association as agricultural fertilizers by DGGE analysis. The application of this technique was efficient for evaluating treatments, as well as, confirming the treatments contribution for increasing bacterial diversity in soil samples. The third chapter compared the cultivation technique sensitivity for American Foul Brood (AFB) diagnosis, a bee disease caused by Paenibacillus larvae, to cultivation independent technique by PCR. This survey demonstrated that sensitivity of the technique was greater than that one by P. larvae cultivation technique. Thus, the present survey concluded that cultivation independent techniques were useful for granting agriculture sectors demands. / O conhecimento das informa??es gen?ticas bacterianas tem permitido avan?os em diversas ?reas do conhecimento. Esse conhecimento permite detectar microrganismos, bem como avaliar a produ??o de fatores de virul?ncia e resist?ncia em determinadas popula??es. Mais recentemente, o diagn?stico molecular pela detec??o de fragmentos de DNA espec?ficos desses agentes vem sendo implementado em situa??es onde o cultivo bacteriano n?o ? vi?vel ou ? dificultado por condi??es bi?ticas ou abi?ticas. Al?m da detec??o de agentes espec?ficos, o conhecimento da diversidade bacteriana presente em alguns ambientes pode ser um importante crit?rio, como, por exemplo, para avaliar a qualidade do solo. A forma mais comum de avaliar a diversidade bacteriana se d? atrav?s do cultivo. Por?m, a maior parte das popula??es bacterianas presentes no solo n?o s?o cultiv?veis, com isso, a utiliza??o de ferramentas moleculares s?o cada vez mais utilizadas nesse tipo de an?lise. O presente estudo teve como objetivo padronizar a utiliza??o de t?cnicas independentes de cultivo para atender demandas de tr?s setores da agricultura. O primeiro cap?tulo padronizou a utiliza??o da t?cnica de DGGE (Eletroforese em Gel de Gradiente Desnaturante) para an?lise da diversidade bacteriana do leite de tetos mast?ticos e sadios de um mesmo animal. Os resultados apontaram para diferen?as no perfil bacteriano dos dois grupos analisados. O segundo cap?tulo avaliou o impacto sobre a microbiota bacteriana em solos tratados com associa??es de cascalho de perfura??o e tortas de mamona e crambe como adubos agr?colas atrav?s da an?lise de DGGE. A aplica??o da t?cnica de DGGE foi eficiente na avalia??o e confirmou que os tratamentos contribu?ram para o aumento da diversidade bacteriana nas amostras de solos estudadas. O terceiro experimento comparou a sensibilidade da t?cnica de cultivo, oficial para diagn?stico da Cria P?trida Americana (CPA), uma doen?a ap?cola causada por Paenibacillus larvae, com t?cnica de independente de cultivo por PCR do material extra?do direto do mel. Este estudo constatou que a sensibilidade da t?cnica proposta foi superior ? obtida pela t?cnica de cultivo de P. larvae. Com isso, conclui-se que as t?cnicas independentes de cultivo foram ?teis no atendimento das demandas das quest?es dos setores agr?colas contemplados no estudo.

Bakgrund: Behandling med parenteral nutrition innebär att näringsbehovet tillgodoses utan förtäring av föda. Behandlingen ses av anhöriga som livsavgörande och av sjuksköterskor som avancerad och resurskrävande. Syfte: Att beskriva patienters upplevelser av sin livssituation i samband med parenteral nutritionsbehandling i hemmet. Metod: En systematisk litteraturstudie med kvalitativ ansats och beskrivande syntes, där tio artiklar analyserades. Resultat: Analysen resulterade i två teman och fem subteman. De teman som framkom i resultatet var Att uppleva förändringar i vardagen och Att hantera den nya vardagen. Patienterna upplevde fysiska och sociala begränsningar samt att de var i behov av stöd från anhöriga och sjukvården. De upplevde även en oro och rädsla för komplikationer. Trots detta var patienterna tacksamma till behandlingen då den medförde en större frihet och kontroll. Slutsats: Behandling med parenteral nutrition i hemmet medför förändringar i det dagliga livet. Det innebär både möjligheter och begränsningar där patienterna uttrycker ökat behov av stöd inom flera områden. Därmed är det av betydelse för sjuksköterskor att skapa en ökad förståelse om hur patienterna upplever sin livssituation för att kunna erbjuda god omvårdnad. / Background: Treatment with parenteral nutrition means that the nutritional need is satisfied without eating. The treatment is viewed upon by relatives as life-saving and by nurses as advanced and demanding. Aim: To describe patients ́ experiences of their life situation in association with home parenteral nutrition. Method: A qualitative systematic literature study with descriptive approach, where ten articles were analyzed. Results: The analysis resulted in two themes and five subthemes. The themes that emerged was To experience changes in the daily life and To handle the new daily life. The patients experienced that they were physically and socially restricted and in need of support from relatives and healthcare. They also experienced anxiety and fear of complications. The patients were grateful despite all of this because the treatment offered them more freedom and control. Conclusion: Treatment with home parenteral nutrition resulted in changes in their everyday life. This brings possibilities and restrictions where the patients expressed an increase of support in many different areas. It is therefore important for nurses to increase their knowledge of how the patients perceive their life situation to be able to offer better care.

Em virtude dos danos cognitivos, sensório motores e psicossociais causados pelo transtorno mental no funcionamento ocupacional, a qualidade de vida dos sujeitos é deteriorada e o isolamento e a inatividade se impõem, acentuando a exclusão. OBJETIVO: Identificar o nível de desempenho em atividades de vida independente de portadores de transtornos mentais e correlacionar variáveis. MÉTODO: Estudo transversal descritivo em dois serviços de saúde mental matogrossenses. Amostra constituída por 146 sujeitos (73 portadores de transtorno mental/ 73 informantes destes). Foi feita a Revisão Integrativa da Literatura sobre atividades de vida independente em portadores de transtorno mental, posteriormente aplicado um questionário sociodemográfico e o Inventário de Habilidades de Vida Independente (ILSS-BR) para identificar áreas da vida independente potencialmente prejudicadas. ANÁLISE DOS DADOS: Análise e correlações pelo STATA. Escores obtidos equiparados de acordo com a pontuação do ILSS, no escore global e subescalas.RESULTADOS: Maioria mulheres (71,2%), casados (63%), 35 a44 anos (46%), baixo nível escolar (48% concluíram quarta série), baixo poder de compra (68,6% com renda máxima R$ 1.024,00). Os domínios da vida independente mais comprometidos são Emprego, Lazer e Transporte, que sofreram influência de diagnóstico, tempo de transtorno mental, escolaridade e nível econômico. DISCUSSÃO: Os escores da versão para o informante ficaram em 1,3 (de 0 a 4) e do paciente, em 10,2% (de 0 A 100%), indicando graves limitações em atividades de vida independente e discrepâncias entre a percepção dos dois grupos. Foram correlacionados prejuízo nas atividades de vida independente e baixa escolaridade, baixo nível econômico, desqualificação profissional, maior tendência a abandonar estudos e cursos profissionalizantes. Os homens são mais vulneráveis ao comprometimento e sofrem maior impacto na área laboral. CONCLUSÕES: Os domínios comprometidos são relacionadas à contratualidade e socialização pelas atividades, merecendo intervenções de enfermagem diretas e singulares, macrossociais e empreendidas no habitat / Because of the cognitive, sensory motor and psychosocial damage caused by mental disorders in occupational functioning, quality of life is deteriorating, isolation and inactivity are result, accentuating the deletion. OBJECTIVE: To identify the level of performance in life activities regardless of the mental patients and correlate variables.METHODS: A descriptive cross- sectional study in two Mato Grosso mental health services. Sample of 146 subjects (73 patients with mental disorders / 73 informants of them). Integrative Literature Review was done about independent living activities in patients with mental disorders, afterward to a sociodemographic questionnaire and the Inventory of Independent Living Skills (ILSS-BR) was intended to identify areas of potentially impaired independent living. ANALYSIS: Analysis and correlations by STATA. Similar scores obtained by the score of ILSS, the overall score and subscales. RESULTS: Most women (71.2%), married (63), 44 35 years old (46%), low educational level (48% completed fourth grade), low purchasing power (68.6% with R$1.024,00 maximum income). The more committed areas of independent living are Employment, Leisure and Transport, who have been influenced by diagnosis, duration of mental disorder, education and income. DISCUSSION: The scores for the informant version were 1.3% and patient, at 10.2%, indicating severe limitations in independent living activities and discrepancies between the perceptions of the two groups. Injury were correlated in independent living and low education, low income, greater tendency to abandon studies and vocational courses activities. Men are more vulnerable and suffer greater impact in employment area. CONCLUSIONS: The affected modules are related by contractuality and socialization activities, requiring direct and individuallynursing interventions in macrosocial- habitat

This dissertation analyzes Mennonite missionary engagement with African Independent Churches in West Africa. The engagement between missionaries and indigenous churches gave rise to a novel mission interaction with a non-western form of Christianity. It led to the early development of mission strategy and theory from an intentionally Anabaptist perspective. Based upon close analysis of archival material, the dissertation examines the extended encounter between missionaries and Independents in southeastern Nigeria between 1958 and 1967. It places the encounter within the context of the religious history of both groups and outlines the influence of the experience on subsequent mission work. This case study sheds new light on the emergence of African indigenous Christian movements and western Christians’ interaction with those movements during the period of decolonization and African nationalism. The history that this study constructs shows that the religious and missiological assumptions that each party brought to the encounter complicated their relationship. The Independents’ religious history led them to expect missionaries to establish traditional mission educational and healthcare institutions that would reinforce their well-being. Missionaries Edwin and Irene Weaver and their colleagues were hesitant to do so, since their experience in India had convinced them that such institutions caused dependency on foreign funds and impeded indigenization. They focused, rather, on encouraging better relationships between estranged Independents and mission churches, capacitating Independent churches through biblical training, and reinforcing Independents’ indigenous identity. Yet some Nigerian Independents insisted on a traditional mission relationship and its accompanying Mennonite identity. Missionaries borrowed mission theory about indigenization from the wider missionary movement, but applied and modified it over time, finally incorporating it into an Anabaptist missionary approach for work in Nigeria, Ghana, Cote d’Ivoire and the Republic of Benin. This study suggests that while relationships between streams of the Christian movement are conditioned by their different religious histories and cultures, they nevertheless generate missiological insights. Through this engagement missionaries articulated an Anabaptist missiology that became influential throughout Africa. In turn, the Mennonite missionary presence enabled some Nigerian Independents to network successfully with the world Christian movement via their Mennonite affiliation.

Considerando-se o contexto de gerenciamento energético em grades móveis, neste trabalho foram propostos dois algoritmos de escalonamento (Maximum Regret e Greedy) que, além de minimizar o consumo de energia, visam assegurar o cumprimento dos requisitos de qualidade de serviço das aplicações submetidas pelos usuários. Tais algoritmos foram projetados a partir de soluções heurísticas para o problema de escalonamento ciente de consumo de energia em grades móveis, que foi modelado como um problema de otimização envolvendo variáveis binárias. Por meio de experimentos, que consideraram tanto cenários estáticos quanto dinâmicos, foi demonstrada a viabilidade dos algoritmos de escalonamento propostos em relação à redução do consumo de energia. Em seu pior caso, o algoritmo Maximum Regret foi 12,18% pior que o referencial determinado pela melhor solução do solver Gurobi; já no pior caso do algoritmo Greedy, tal diferença foi de apenas 8,14% / Considering the context of energy management in mobile grids, this work proposes two scheduling algorithms (Maximum Regret and Greedy) that aim not only to reduce the energy consumption of the mobile devices, but also to ensure the QoS (Quality of Service) requirements of the running applications. These algorithms were designed based on heuristics for the energy aware scheduling problem in mobile grids, which was modeled as an optimization problem with integer variables. The performances of the proposed scheduling algorithms were evaluated by an extensive set of experiments, which demonstrated the feasibility of the adopted approach regarding energy consumption minimization. In its worst case, the Maximum Regret algorithm was 12.18% worse than the best solution provided by the Gurobi solver. While in the Greedys worst case the performance difference was just 8.14%

According to a survey, the majority of fourth grade students in 2005 did not choose reading as a preferred activity for entertainment (Guthrie, McRae, & Klauda, 2007). Adolescents are increasingly resistant to reading and seldom list it as a pleasurable activity. Interestingly, research shows that students who enjoy reading more do better academically (Gambrell, 2011). Accordingly, as a teacher I seek to increase students' reading for pleasure. To give space in my curriculum for students to do this and for me to support them, I used Sustained Silent Reading (SSR), a practice where students are given time to read a text of their choosing during class time. Adhering to LaBoskey's (2004) criteria for self-studies, I conducted a self-study of teacher practices. There were two rounds of field notes with critical friend commentary that allowed me to identify types of readers and types of responses. To present my findings, I developed vignettes to capture my field notes about types of readers and I identified field notes that captured general and specific responses to readers for which I provided exemplar on my findings. I also attended to trustworthiness. This study explored what I as a teacher know and learned about increasing my students' engagement with reading for pleasure during SSR time. By categorizing my students' habits and charting my responses and interventions, I was able to understand what practices to use to encourage students to read for pleasure according to their characteristics.

To increase diversity of enzymes and proteins, cells mix and match exonic and intronic regions retained in mature mRNAs by alternative splicing. An estimated 94% of all multi-exon genes express one or more alternatively spliced transcripts generating proteins with similar or modified functions. Cyclooxygenase is a signaling enzyme that catalyzes the rate-limiting step in the synthesis of diverse bioactive lipids termed prostaglandins. Prostaglandins are involved in myriad physiological and pathopysiological processes including vasoregulation, stomach mucosal maintenance, parturition, pain, fever, inflammation, neoplasia and angiogenesis and are inhibited by aspirin-like drugs known as NSAIDs. In 2002 an alternatively spliced, intron-1 retaining variant of COX-1 was cloned from canine brain tissue. This new variant, termed COX-3 or COX-1b, is an enzymatically active prostaglandin synthase expressed at relatively high levels in a tissue and cell type dependant manner in all species examined. In humans and most rodent species intron-1 is 94 and 98 nucleotides long respectively. Retention of the intron in these species introduces a frameshift and is predicted to result in translation of a very small 8-16kD protein with little similarity to either 72kD COX-1 or COX-2, calling into question the role of this variant. In this dissertation, I present my results from cloning and ectopically expressing a complete and accurate COX-3 cDNA from both rat and human. I confirmed that COX-3 mRNA encodes multiple large molecular weight cyclooxygenase-like proteins in the same reading frame as COX-1. Translation of these proteins relies on several recoding mechanisms including cap-independent translation initiation, alternative start site selection, and ribosomal frameshifting. Using siRNA and Western blotting I have identified some of these proteins in tissues and cells. Two COX-3 encoded proteins are active prostaglandin synthase enzymes with activities similar to COX-1 and represent novel targets of NSAIDs. Other COX-3 proteins have unknown function, but their size and cellular location suggest potential roles as diverse as cytosolic enzymes and nuclear factors.

The study examines how independent bookstores differentiated themselves from other bookstore retailers, to satisfy their customer needs and then to achieve customer engagement. The research proposes two questions as following: How do independent bookstores in Uppsala differentiate themselves in the book retailing industry? How do customers engage with independent bookstores? In order to answer these questions, qualitative research method was employed in this study. This research took all of the seven independent bookstores in Uppsala as a sample, interviewed owner/managers of each of them, and collected 85 questionnaires from their customers on the basis of the servicescape theory. The researchers employed the approach of qualitative data analysis. The analysis of the findings was based on Raffaelli’s 3C’s model, servicescape theory, and customer engagement theory. The conclusion of this study illustrates the actions of independent bookstores of differentiating themselves in the six dimensions of servicescape theory and indicates that both independent bookstore and their customers lay emphasis on the differentiation of functionality and employees. Furthermore, the customer engagement could be enhanced through the differentiation.

A fundamental issue in many distributed computing problems is the need for nodes to distinguish themselves from their neighbors in a process referred to as symmetry breaking. Many well-known problems such as Maximal Independent Set (MIS), t-Ruling Set, Maximal Matching, and (\Delta+1)-Coloring, belong to the class of problems that require symmetry breaking. These problems have been studied extensively in the LOCAL model, which assumes arbitrarily large message sizes, but not as much in the CONGEST and k-machine models, which assume messages of size O(log n) bits. This dissertation focuses on finding upper and lower bounds for symmetry breaking problems, such as MIS and t-Ruling Set, in these congested models. Chapter 2 shows that an MIS can be computed in O(sqrt{log n loglog n}) rounds for graphs with constant arboricity in the CONGEST model. Chapter 3 shows that the t-ruling set problem, for t \geq 3, can be computed in o(log n) rounds in the CONGEST model. Moreover, it is shown that a 2-ruling set can be computed in o(log n) rounds for a large range of values of the maximum degree in the graph. In the k-machine model, k machines must work together to solve a problem on an arbitrary n-node graph, where n is typically much larger than k. Chapter 4 shows that any algorithm in the BEEP model (which assumes 'primitive' single bit messages) with message complexity M and round complexity T can be simulated in O(t(M/k^2 + T) poly(log n)) rounds in the k-machine model. Using this result, it is shown that MIS, Minimum Dominating Set (MDS), and Minimum Connected Dominating Set (MCDS) can all be solved in O(poly(log n) m/k^2) rounds in the k-machine model, where 'm' is the number of edges in the input graph. It is shown that a 2-ruling set can be computed even faster, in O((n/k^2+ k) poly(log n)) rounds, in the k-machine model. On the other hand, using information theoretic techniques and a reduction to a communication complexity problem, an \Omega(n/(k^2 poly(log n))) rounds lower bound for MIS in the k-machine model is also shown. As far as we know, this is the first example of a lower bound in the k-machine model for a symmetry breaking problem. Chapter 5 focuses on the Max Clique problem in the CONGEST model. Max Clique is trivially solvable in one round in the LOCAL model since each node can share its entire neighborhood with all neighbors in a single round. However, in the CONGEST model, nodes have to choose what to communicate and along what communication links. Thus, in a sense, they have to break symmetry and this is forced upon them by the bandwidth constraints. Chapter 5 shows that an O(n^{3/5})-approximation to Max Clique in the CONGEST model can be computed in O(1) rounds. This dissertation ends with open questions in Chapter 6.

The purpose of this study was to examine the changes in running economy of collegiate cross-country runners with 6 weeks of training on the PowerCranks ™ independent bicycle crank. Thirteen collegiate cross-country runners completed the study. Participants were asked to perform 6 weeks of training with either the PowerCranks™ device or the standard cranks (control group). Participants trained 3 days per week with a 48-hour minimum rest time between training sessions. Pre- and post-running economy and V02 max test data were collected. Data were analyzed with SPSS version 19 using a paired-samples t test as well as an independent t test. The paired samples I-test results for the participants pre-training running economy in the PowerCranks™ group were 2.98 ± 0.60 Llmin to 3.08 ± 0.59 Llmin post training; p = 0.057, 1= -2.464. The control group for the PowerCranks™ results were 2.68 ± 0.51 Lfmin pre-training, with post training results of2.69 ± 0.57 L/min,p = 0.815, t = -.245. Results for pre-training V02 max were 4. 10 ± 0.72 Llmin to 4.17 ± 0.75 Lll11in post training; p = 0.230, [= -1.366 with the PowerCranks™ group. The control group [or V02 max. results wen: 3.83 ± 1.10 Llmin pre-training with post-training resulting as 3.92 ± 1.09 Llmin,p = .245, t = -1.287. The results of the independent t test also showed that there was no significant change in values for running economy (t = -.112, P = .913) or V02 max (/ = 1.569,p = .145) when PowerCranks™ and control groups were compared. It was concluded that within the limitations of this study, experienced collegiate numers who performed 6 weeks of cross training with the PowerCranks™ independent cycle crank displayed no significant difference in running economy or VOz max results post training.