O presente trabalho teve como objetivo estudar o efeito que a alteração na sensibilidade ou metabolismo hormonal em uma planta hospedeira poderia ter sobre o desenvolvimento de micorrizas arbusculares (MAs). Para tal, uma coleção de mutantes hormonais de tomateiro (Lycopersicon esculentum) introgredidos na cultivar miniatura Micro-Tom foi inoculada com o fungo Glomus clarum, em dos níveis de P no substrato. Foram realizados 3 experimentos, com os genótipos Never ripe (parcialmente insensível ao etileno), epinastic (superprodutor de etileno), bushy root (parcialmente insensível a citocinina), diageotropica (parcialmente insensível a auxina), procera (supersensível a giberelina) e notabilis (deficiente em ABA). Os parâmetros avaliados foram: porcentagem de colonização micorrízica, massa seca da parte aérea e massa seca da raiz em condições de P suficiente e insuficiente. Com os genótipos que apresentaram maiores alterações no desenvolvimento de MAs, Never ripe e epinastic , foram conduzidas análises morfológicas por meio de microscopia de luz, dosagem de P na parte aérea e estudos de expressão gênica através da técnica de PCR em tempo real (PCR Real-Time). Concluiu-se que, nas condições do presente trabalho, o grupo hormonal que mostrou a maior influencia sobre a formação de MAs foi o etileno, sendo que o seu efeito parece ser tanto estimulatório quanto inibitório. Contudo, os resultados de expressão de genes de defesa não permitem explicar as diferenças observadas. / The aim of the present work was to study the effect that alterations in hormonal sensitivity and metabolism in a host plant could have in the development of arbuscular mycorrhizae (AM). In this regard, a series of hormone-related mutants introgressed in the tomato (Lycopersicon esculentum) cultivar Micro-Tom were inoculated with the fungus Glomus clarum, in two different levels of substrate P. Three experiments were performed, using the genotypes Never ripe (partially insensitive to ethylene), epinastic (ethylene overproducer), bushy root (partially insensitive to cytokinin), diageotropica (partially insensitive to auxin), procera (gibberellin hypersensitive) and notabilis (ABA-deficient). The following parameters were assessed: mycorrhizal colonization percentage, shoot and root dry mass, under conditions of either sufficient or insufficient P on the substrate. Further analyses, such as root morphology, P dosage and gene expression quantification (through Real-Time PCR), were performed on the genotypes which presented the most alterations in mycorrhizal development, namely Never ripe and epinastic. It was concluded that the hormone showing most influence on AM formation was ethylene. Its effect appears to be either stimulatory o inhibitory. In any case, defense gene expression alone could account for the observed differences.

Micorrizas arbusculares (MAs) são simbioses mutualistas formadas entre fungos do solo e a maioria das plantas terrestres, principalmente angiospermas. Esta simbiose pode trazer uma série de benefícios para o hospedeiro vegetal, como uma maior absorção de fosfato, um aumento na tolerância ao estresse abiótico e biótico, e resistência a agentes patogênicos, resultando numa melhor adaptação ao meio. É possível que hormônios vegetais estejam envolvidos em eventos de sinalização entre fungos micorrízicos arbusculares e plantas hospedeiras, durante os processos de colonização das raízes e de desenvolvimento dos arbúsculos. Recentes estudos têm demonstrado que durante o estabelecimento da simbiose, ocorre uma reprogramação da expressão de um grande número de genes, e que essas alterações na expressão gênica podem estar associadas a alterações dos níveis de hormônios nas plantas. O objetivo deste trabalho foi avaliar o desenvolvimento de MAs em mutantes de tomateiro cv Micro-Tom (MT) insensível ao ácido jasmônico (jai1-1), superprodutor de etileno (epi) e duplo mutante epijai1 inoculados com Glomus clarum em condições de baixa concentração de fósforo no substrato, e a expressão de genes relacionadas à biossíntese do ácido jasmônico (AJ) (OPR3 e AOC) e do etileno (ET) (ACO4 e ACS2). O mutante jai1-1 não apresentou colonização intrarradicular e o acúmulo de transcritos tanto para a via de biossíntese do AJ como do ET foi relativamente menor do que o controle MT o que sugere a importância do AJ para o desenvolvimento da simbiose.O mutante epi e o duplo mutante apresentaram colonização intraradicular reduzida em relação ao controle MT. O acúmulo de transcritos dos genes da via do ET (ACO4 e ACS2) e do AJ (OPR3 e AOC) foi maior no mutante epi. A insensibilidade ao AJ presente no duplo mutante não impediu o acúmulo de transcritos para as vias de biossíntese do AJ e do ET, o que sugere uma relação sinérgica entre os hormônios vegetais no estabelecimento da simbiose. / Arbuscular mycorrhizal (AM) symbioses are formed between mutualistic soil fungi and most land plants, particularly angiosperms. This symbiosis can bring many benefits to the host plant, such as increased absorption of phosphate, an increase in tolerance to abiotic and biotic stress, and resistance to pathogens, resulting in better adaptation to the environment. Its possible that plant hormones are involved in signaling events between mycorrhizal fungi and host plants during the processes of colonization of roots and development of arbuscules. Recent studies have shown that during the establishment of symbiosis, there is a reprogramming of the expression of a large number of genes, and that these changes in gene expression may be associated with altered levels of hormones in plants. The aim of this study was to evaluate the development of MA in mutants of tomato cv Micro-Tom (MT) insensitive to jasmonic acid (jai1-1), ethylene overproducer (epi) and double mutant epijai1 inoculated with Glomus clarum under low phosphorus concentration in substrate, and the expression of genes related to biosynthesis of jasmonic acid (JA) (and OPR3 AOC) and ethylene (ET) (ACO4 and ACS2). Jai1-1 mutant showed no intraradical colonization and the accumulation of transcripts for both the biosynthesis of JA and ET was less than the control MT suggesting the importance of JA for the development of epi and double mutant symbiosis. The mutant epi and the double mutant showed reduced intraradical colonization when compared to the control MT. The transcript accumulation of the ET (ACO4 and ACS2) and JA (OPR3 and AOC) genes was higher in the mutant epi. The insensitivity to the JA in this double mutant did not prevent the accumulation of transcripts for the biosynthetic pathways of JA and ET, suggesting a synergistic relationship between plant hormones for the establishment of symbiosis.

Made available in DSpace on 2016-03-15T19:47:27Z (GMT). No. of bitstreams: 1 Rosalina Brites de Assuncao.pdf: 1666468 bytes, checksum: d06f2a575fae1e9205521169ae939a22 (MD5) Previous issue date: 2011-12-16 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / This paper intends to investigate how an obligatory tone is constructed in the texts that materialize the Landless Workers Movement (MST) and the Ministry of Education and Culture (MEC) discourse about an educational proposal to the rural population. Our corpus is based on five texts that belong to the Dossiê MST School collection and two of the National Council of Education/MEC Resolutions: Resolution CEB/CNE nº 1/2002 and Resolution CEB/CNE nº2/2008. At first, the aim is to analyze the discourse strategies that both enunciators used in their discourses, to produce a demanding tone in the enunciation, permitting to outline the image which they construct of themselves in the discourse. Secondly, it seeks to discover, through the analysis of the heterogeneity enunciative of each discourse, the ideological position of the enunciators and to reveal their intention within their education proposal. The analysis was made according to the theoretical assumptions of the French line of the Discourse Analysis as presented in Maingueneau (2004, 2008), Orlandi (2007), Brandão (2004), Amossy (2005). The issues that focus on enunciation projection in the statements are linked to the ideas of the Enunciation theory, based on the studies of Benveniste (1989), Authier-Revuz (2004), Fiorin (1999). The linguistic modalization analysis is subsidized by the Functionalist theory according to Halliday (1985), Hengeveld (2004), Palmer (1986) and Neves (2007). The results demonstrate that the idea of obligation shown in both discourses is constructed in a different way. The enunciative organization shows that, in the MST discourse, the tone of obligation is more impositive, which at discourse surface, behaves as an emancipation role. In the governments discourse, MEC, the tone is less mandatory and has the paper to include the countrymen in the culture and values of the burgeoise society. However, the hypothesis which is intended to be proved is that the option for the mandatory tone used by both enunciators is a discourse strategy which is constituted as by a power dispute between equality and difference, in which the MEC discourse intends to silence the MST movement and the MST aims to unstable the State s Education proposal. / A presente pesquisa pretende investigar como se constrói o tom de obrigatoriedade em textos que materializam o discurso do MST e o do MEC sobre uma proposta de educação para a população do meio rural. Tomam-se como corpus de pesquisa cinco textos pertencentes à coletânea Dossiê MST Escola e duas Resoluções do Conselho Nacional de Educação/MEC: Resolução CEB/CNE nº 1/2002 e Resolução CEB/CNE nº2/2008. Num primeiro momento, busca-se analisar as estratégias discursivas que os enunciadores de ambos os discursos mobilizam para imprimir no enunciado o tom de obrigatoriedade que permite delinear a imagem que constroem de si no discurso. Num segundo momento, objetiva-se verificar, mediante a análise da heterogeneidade enunciativa de cada discurso, o posicionamento ideológico dos enunciadores e desvelar a intencionalidade de suas propostas de educação. Como fundamentação teórica para as análises adotam-se os pressupostos teóricos da análise do discurso de linha francesa, conforme os estudos de Maingueneau (2004, 2008), Orlandi (2007), Brandão (2004), Amossy (2005). Intervêm noções ligadas à teoria da enunciação, pelas quais se focalizam as questões da projeção da enunciação no enunciado, conforme os estudos de Benveniste (1989), Authier-Revuz (2004), Fiorin (1999). Para a análise da modalização linguística consideram-se os fundamentos da teoria funcionalista da linguagem, segundo Halliday (1985), Hengeveld (2004), Palmer (1986) e Neves (2007). Os resultados da análise apontam que a noção de obrigatoriedade nos dois discursos é construída de maneira diferenciada. No discurso do MST, a forma de organização dos enunciados revela que o tom de obrigatoriedade é mais impositivo, desempenhando, na superfície do discurso, um papel emancipatório. No discurso do MEC, esse tom é menos autoritário e tem o papel de incluir o homem do campo na cultura e nos valores da sociedade burguesa. Entretanto a hipótese que se procura comprovar é a de que a opção pelo tom de obrigatoriedade por ambos os enunciadores é uma estratégia discursiva que se constitui por um jogo de poder entre igualdade e diferença, em que o discurso oficial busca silenciar o discurso do movimento e o discurso do MST visa a desestabilizar a proposta de educação do Estado.

A regeneração in vitro é bastante utilizada em processos biotecnológicos. No entanto, pouco se conhece sobre os mecanismos envolvidos na aquisição de competência para formação de novos órgãos. Em tomateiro (S. lycopersicum), a alta capacidade de regeneração in vitro é atribuída ao alelo Rg1, vinda de Solanum peruvianum, e que está presente na cv MsK (S. lycopersicum x S. peruvianum). Os genes de nanismo da cv Micro-Tom (MT) foram passados para MsK, obtendo-se, após 8 gerações de autofecundação (F8), a cv Micro-MsK. No presente estudo, após 6 gerações de retrocruzamentos (BC6Fn), criou-se MT-Rg1, com Rg1 isogênico a MT. Testes de regeneração in vitro mostraram que a formação de gemas caulinares adventícias para MT-Rg1 e Micro-MsK são equivalentes, evidenciando que a alta capacidade de regeneração de Micro-MsK é basicamente devido ao Rg1. Plantas MTRg1 apresentam freqüente formação de 3 cotilédones, excesso de ramificações caulinares e senescência atrasada, características ausentes nos parentais. Esses efeitos pleiotrópicos de Rg1 coincidem com aqueles descritos para alterações no hormônio citocinina. Testes de sensibilidade à citocinina e de tempo de senescência realizados por nós, bem como dosagem desse hormônio feita por outras equipes, descartaram essa hipótese. Enxertias recíprocas evidenciaram que a promoção da ramificação caulinar por Rg1 não é um sinal translocável, mas parece ser uma característica intrínseca do tecido. Rg1 também promoveu uma maior formação de raízes adventícias em estacas ex vitro, sugerindo que ele afeta o processo de competência para formação de órgãos em geral, e não somente a indução específica de gemas caulinares. Duplos mutantes entre Rg1 e mutantes com alteração na sensibilidade/metabolismo hormonal (dgt, brt e pro), ausência de ramificações caulinares (ls) e senescência acelerada (l) foram obtidos e testados quanto ao padrão de ramificações e à capacidade de regeneração in vitro. Além desses parâmetros, a presença de Rg1 suprimiu o fenótipo de folhas pouco recortadas do mutante hipersensível a giberelina (pro) e recuperou o sistema radicular pouco desenvolvido do mutante com baixa sensibilidade à auxina (dgt). Rg1 também foi capaz de reverter a ausência de ramificações laterais de ls, sendo que a mutação Me, o qual representa uma superexpressão de um gene do tipo KNOX, não foi capaz. Esse resultado sugere que Rg1 não é um gene do tipo KNOX, embora esses genes sejam considerados os principais controladores da competência. Analisando diferentes tipos de explantes em experimentos independentes, contatou-se que, em geral, as mutações brt (baixa sensibilidade a citocinina), dgt e ls diminuíram a capacidade de regeneração de Rg1, enquanto l aumentou. Rg1 mostrou-se particularmente epistático à mutação pro, revertendo o fenótipo de baixa formação de gemas caulinares desse mutante. Surpreendentemente, Rg1 provou ser mais sensível à auxina em testes de alongamento de segmentos de hipocótilos, sendo capaz de reverter o fenótipo do mutante dgt nesse mesmo teste. Em conjunto, esses resultados confirmam o papel de Rg1 na fase de aquisição da competência e sugerem uma interação dos hormônios giberelina e auxina nesse processo. / The In vitro regeneration process is widely used in plant biotechnology. However, the mechanisms involved in the acquisition of competence for organ formation are hitherto unknown. In tomato (S. lycopersicum), the high capacity for in vitro regeneration is attributed to the Rg1 allele from S. peruvianum, which is present in the cv MsK (S. lycopersicum x S. peruvianum). The dwarfism genes of the cv Micro-Tom (MT) were transferred to MsK, and, after 8 generations of selfing (F8), the cv Micro-Msk was obtained. Here, after 6 generations of backcrosses (BC6Fn), we created the MT-Rg1, which has Rg1 isogenic to MT. Tests of in vitro regeneration showed that shoot formation in MT-Rg1 and Micro-MsK are equivalent, suggesting that the high capacity of regeneration of Micro-MsK is basically due to Rg1. Comparing MT-Rg1 with the control MT, we noticed a high frequency of 3 cotyledon formation, increased shoot branching and late senescence, which are absent in the parentals. These pleiotropic effects of Rg1 coincide with those described for plants with alterations in the hormone cytokinin. Tests of sensitivity for cytokinin and senescence behavior carried out by us, as well as dosage of that hormone made by other researches, discarded that hypothesis. Reciprocal grafting showed that the promotion of shoot branching by Rg1 is not a transmissible signal, but seems to be an inherent characteristic of the tissue. Rg1 also increases adventitious roots formation of ex vitro cuttings, suggesting that it affects the process of competence, which is common for shoots and roots, instead of the specific induction of shoots. Double mutants between Rg1 and mutants with alteration in the sensitivity/metabolism of plant hormones (dgt, brt and pro), as well as mutants with absence of shoot branching (ls) and accelerated senescence (l) were obtained and tested for the capacity of in vitro regeneration. The presence of Rg1 suppressed the phenotype of less dissected leaves of the mutant hypersensitive to gibberrellin (pro) and rescued the phenotype of poor developed root system of the mutant with low auxin sensitivity (dgt). Rg1 was also able to revert the absence of axillary shoot formation in ls, whereas the mutant Me, which represents an over expression of a KNOX gene was not. This result suggests that Rg1 is not a KNOX gene, although those genes are considered the main controllers of the competence. Analyzing different types of explants in independent experiments, it was verified that, in general, the mutations brt (low sensibility to cytokinin), dgt and ls decreased the regeneration capacity of Rg1, while l increased it. Rg1 was particularly epistatic to the pro mutation, reverting the phenotype of little shoot formation of that mutant. Surprisingly, Rg1 proved to be more sensitive to auxin in the hypocotyl segment elongation test, being able to revert the phenotype of the mutant dgt in the same test. Together, these results confirm the role of the Rg1 in the process of acquisition of the competence and suggest an interaction of the hormones gibberellin and auxin in this process.

Book Summary: The Grove Dictionary of American Music, second edition is the largest, most comprehensive reference publication on American Music. Twenty-five years ago, the four volumes of the first edition of the dictionary initiated a great expansion in American music scholarship. This second edition reflects the growth in scholarship the first edition initiated. At eight volumes, it provides greatly expanded coverage, particularly in the areas of popular music, cities and regions, musical theater, opera, concert music, and music technology, as well as the musical traditions of many ethnic and cultural groups.

Despite the broadening of the subject matter of English drama in its ???new wave??? period from the late nineteen fifties, it is striking to see how much of enduring mainstream English drama has a domestic focus. The purpose of this thesis is to provide the first full-length study of marriage on the English stage from 1930-1990. The thesis examines the way in which a number of important playwrights have fashioned drama from the conflict between the public, or institutional, functions of marriage and the private, or relational, functions of marriage. The thesis places this conflict into historical context. This will show that the conflict between the private and public aspects of marriage is not one of clearly opposed opposites but one that is made dynamic by significant social and legal changes to the status, function and conventions of marriage. The thesis also demonstrates that this conflict is further complicated by class considerations and by the particular circumstances of each marital partnership. From the wealth of material available, I have chosen to examine in detail the work of seven playwrights who have made significant contributions to domestic drama or domestic comedy. Playwrights have been selected because their plays gained a strong audience on first performance and because, through numerous revivals and through publication of scripts, they have earned an enduring place in English drama. John Osborne???s Look Back in Anger, which in 1956, ushered in the ???new wave???, is pivotal play for discussion. The previous generation is represented by Noel Coward, J. B. Priestley and Terence Rattigan. The ???new wave??? and its aftermath are represented by Harold Pinter, Tom Stoppard and, finally Alan Ayckbourn. Despite a wide variety of approaches to the topic of marriage, these writers tend to assume a middle class audience and to follow or adapt the traditions of realism or comedy of manners. This thesis argues that despite real, even radical, changes to marriage, to accepted sexual practices and to the status of women in the sixty years under discussion, the mainstream theatre has tended to be conservative in its presentation of marital and sexual matters, especially in continuing to reinscribe a public/private opposition determined by gender.

The study elucidates the relation of Tom Stoppard's play Rosencrantz and Guildenstern Are Dead to Hamlet on the one hand and to the Theatre of the Absurd on the other. The two plays chosen to represent the Theatre of the Absurd are Samuel Beckett1 s Waiting for Godot and Harold Pinter* s The Caretaker. Since Stoppard is admired as a master craftsman of language, the emphasis is on his use of language. The extent to which the use of the cliché characterizes the three absurd plays is examined. It is found that the language area covered by the term cliche is not clearly defined and that the term is not uniformly applied. The inquiry centres on finding features, such as repetition, music-hall passages and "ready-made" language, that could explain why the dialogue in the three plays might appear cliche-ridden and on comparing the three plays in respect of these features. 　 The study further draws parallels between Stoppard's play and Waiting for Godot in the use of various techniques, such as misunderstandings, anticlimax and afterthought. It is found that there is often a conscious adoption by Stoppard of Beckett's techniques. To clarify the relation of Stoppard's play to Hamlet various aspects of the two plays are studied. These aspects include changes introduced into stereotyped expressions, punning, the use of parody and the handling of two specific motives, madness and death. Parallels are found in spite of the fact that several centuries separate the two plays, not least in respect to style, technique and language. / digitalisering@umu

In this study, the development of a second-order false belief task is investigated by considering the impact of the acquisition of Turkish evidential markers, namely &ndash / DI (direct evidence) and &ndash / mIs (inference or hearsay). A neutral version of the tasks served as a control form. 21 kindergarten children (aged 4-5 years), 47 primary school children (aged 6- 12 years) and 10 adults participated in the study. Our results revealed that there is no effect of acquisition of evidentials on false belief understanding. Together with the other studies, there is a facilitative effect of &ndash / DI (direct evidence) in understanding of stories/narratives in general rather than false belief understanding for the children at the age of 4 to 6/7. In addition to the second-order false belief tasks (FBT_2), a simple working memory task (WST), a complex working memory task (LST), a perspective taking task (PTT) and a double- embedded relative clause task (REL_2) were used in order to investigate the developmental trend of these tasks and their possible relationship with second-order false belief understanding. Also, to the best of our knowledge this is the first time that a REL_2 task has been devised in a Turkish study. The general developmental trend was found for all tasks. Even if some significant correlations were found for FBT_2 score predicted from other tasks, analyses showed that only the contribution of age was significant. Since all of these domains are not related to second-order false belief reasoning but develop at the same time, it is not incompatible with the serial bottleneck hypothesis. In sum, the findings are matching with the modularity view that ToM is a faculty of the human mind at their own pace that does not share intrinsic content with other faculties such as language and working memory (Leslie et al., 2004). However, it develops together with those other faculties and they may constrain the expression of child

Hur vi än lever våra liv så påverkar det alltid naturen runt omkring oss på något sätt. Bland annat bygger vi hus och infrastruktur, odlar, konsumerar och reser, vilket i olika utsträckningar inverkar på naturen. Idag pågår en aktiv global debatt om de negativa konsekvenserna som människors fotavtryck har satt på Jorden i form av miljöförstöring. Miljöförstöringen handlar till stor del om effekterna av Jordens ökande medeltemperatur, vilket i sin tur i stor utsträckning beror på våra levnadsvanor i form av produktions- och konsumtionsmönster. Ett internationellt arbete har påbörjats sedan en tid tillbaka för att skapa hållbara samhällen. I det här arbetet jämförs två aktörers texter angående deras relation till naturen. Aktörerna är Mohawkindianen Tom Porter samt Förenta Nationernas tre styrdokument: Riodeklarationen, Agenda 21 samt Skogsprinciperna, och de är båda deltagare i den globala miljödebatten. Deras relation till naturen kommer även att sättas i relation till världsbilder och kulturella kontexter samt förståelse kring texternas uppkomst. Resultatet visar att de två aktörernas relation till naturen och världsbilder i stort sett är varandras motsatser, vilket kan förstås utifrån bakgrunden till respektive kulturella kontexter där skapelseberättelsen och den vetenskapliga revolutionen är av betydelse. Även orsaken till texternas uppkomst utgör skillnader som bottnar i hur människans relation till naturen gestaltar sig.

L'import d'ARN dans les mitochondries est un processus ubiquitaire chez les eucaryotes. Dans Saccharomyces cerevisiae un isoaccepteur cytosolique de l'ARNtLys, l'ARNtLys(CUU) (tRK1), est partiellement importé dans les mitochondries. L'adressage vers la surface mitochondriale a été étudié en détail mais l'étape de translocation dans les mitochondries reste toujours à démontrer. L'objectif principal de ce travail de thèse était l'identification et la caractérisation des protéines qui participent dans ce processus. Deux protéines de la membranes externe capables de former des canaux, Tom40 et VDAC1, ont été identifiées et leur rôle dans l'étape de translocation a été évalué in vitro et in vivo en utilisant des souches mutantes appropriées ou des agents capables de bloquer de manière spécifique les canaux formés par les protéines Tom40 et VDAC1. Ainsi il a été démontré que la délétion de VDAC1 ou l'inhibition du canal VDAC1 a conduit à une inhibition importante, néanmoins pas complète, de l'import de tRK1. Le blocage simultané des canaux Tom40 et VDAC1 par contre a causé un arrêt complet de l'import de tRK1 in vitro. Vu ces résultats, nous proposons que la translocation de tRK1 à travers la membrane mitochondriale externe puisse suivre deux chemins alternatifs.