Ultrashort pulsed lasers have become critical to understanding light-matter interactions in new regimes such as generation of attosecond pulses, laser filamentation, and intense relativistic processes. Development of more powerful and energetic ultrafast lasers is required for advancing these fields of study. Several petawatt class systems now exist with more in development to further scale peak power and extend the frontier of ultrafast laser technology. Another relevant solution to the scaling of energy and power of ultrashort pulses is coherent beam combining (CBC). CBC is useful for not only scaling of laser parameters but also to mitigate parasitic nonlinear processes associated with high-intensity ultrashort pulses. In addition CBC is flexible and can be implemented as part of other techniques for ultrashort pulse amplification such as optical-parametric chirped-pulse amplification (OPCPA). In this thesis, CBC of ultrashort laser pulses is investigated based upon the method known as divided-pulse amplification (DPA). Active, passive and hybrid DPA have been achieved in a flashlamp-pumped Nd:YAG laser seeded from a Ti:sapphire mode-locked laser. Picosecond pulses at a repetition rate of 2.5 Hz were amplified and combined to record energy of 216 mJ with a combination efficiency of 80%. Engineering of the Nd:YAG amplifier chain for high-efficiency energy extraction is presented. In addition, phasing of actively divided pulses with a CW pilot laser co-propagating with the pulsed beam is also demonstrated. Analysis of multiple DPA configurations shows the viability of the method for a variety of different laser architectures including discussion of design restrictions.

An alarming increase of antibiotic-resistant bacterial strains has made the demand for novel antibacterial agents, for example, more effective antibiotics, highly crucial. One of the oldest antimicrobial agents is elementary silver which has been used for thousands of years. Even in our days, elementary silver is used for medical purposes, such as for burns, wounds, and microbial infections. We have taken the effectiveness of elementary silver into consideration to generate novel antibacterial and antifouling agents. Our innovative antibacterial agents are hybrid peptide silver nanoparticles (CH-01-AgNPs) that are created de novo and in situ from a silver nitrate solution (AgNO3) in the presence of ultrashort self-assembling peptides compounds. The nucleation of CH-01-AgNPs is initiated by irradiating the peptide solution mixed with the AgNO3 solution using ultraviolet (UV) light at a wavelength of 254 nm, in the absence of any reducing or capping agents. Obviously, the peptide itself serves as the reducing agent. The ultrashort peptides are four amino acids in length with an innate ability to self-assemble into nanofibrous scaffolds. Using these ultrashort peptides CH-01 we were able to create hybrid peptide silver nanoparticles CH-01-AgNPs with a diameter of 4-6 nm. The synthesized CH-01-AgNPs were further characterized using ultraviolet-visible spectroscopy, transmission electron microscopy, dynamic light scattering, and X-ray photoelectron spectroscopy. The antibacterial and antifouling activity of CH-01-AgNPs were then investigated using either gram-negative bacteria, such as antibiotic-resistant Top10 Escherichia coli and Pseudomonas aeruginosa PDO300, or gram-positive bacteria, such as Staphylococcus aureus CECT 976. The hybrid nanoparticles demonstrated very promising antibacterial and antifouling activity with higher antibacterial and antifouling activity as commercial silver nanoparticles. Quantitative Polymerase Chain Reaction (qPCR) results showed upregulation of stress-related genes, e.g. osmB and bdm. Biocompatibility studies of CH-01-AgNPs, using concentrations of 0.06 mM and 0.125 mM, testing for the viability of human dermal fibroblast neonatal (HDFn) cells, showed no significant influence on cell viability. In summary, we consider hybrid peptide silver nanoparticles CH-01-AgNPs as promising biomaterials that can be utilized in various biomedical applications, in particular for wound healing and biofilm inhibition, but also for other applications, such as tissue engineering, drug delivery, regenerative medicine, and biosensing.

<p>Mechanical instruments, such as saw and bur are commonly used for bone cutting during orthopedics surgeries. These conventional instruments showed good bone removal efficiency. Nonetheless, there are some issues with the use of the mechanical tools, such as ill-placed screws and elevation of tissue temperature, which results in thermal damage to the surrounding tissues. These difficulties accompanied with using mechanical tools led to laser ablation investigations. Lasers, including continues wave (CW) and pulsed, were considered to be a promising tool for bone ablation. When compared to mechanical tools, lasers produce less thermal damage to the surrounding tissues due to their ability to focus on a very small spot, which also produces more precise ablation. Lasers also produce no significant mechanical vibrations within the surrounding tissue and thus less mechanical damage and cracks occur during ablation. Performances of laser ablations are measured by several factors; such as collateral damage, machining time, ablated depth, and ablative precision. In this thesis work, a literature review was conducted with the aim of understanding the bone characteristics that are related to the optical properties of bone, which leads to a better understanding for ablation mechanisms. This helps in a proper choice of laser parameters for a certain tissue ablation, and thus avoiding collateral damage.</p> <p>Some laser parameters (pulse energy, scanning speed, and number of passes) were characterized as a first step towards producing large holes. The effect of each one of these laser parameters on the groove depth was found. The feasibility of the ultrafast laser in creating large scale holes was examined, using two scanning strategies: (i) concentric circles scanning, the largest crater depth measured using this procedure was 3.81 mm, (ii) helical scanning, which was used to reduce the machining time, using this procedure a micropillar was created with 12 passes in just 2.5 minutes.</p> / Master of Applied Science (MASc)

A numerical model of the temperature field during pulsed laser cutting of thin sheets (approximately 2.5 x l0⁻⁵ m) was developed. Cutting was simulated through removal of nodes from a finite difference scheme based on sensible heating to the phase change temperature and a single value of latent heat (melting or vaporization). The pulsed laser model predicts a heat-affected zone of less than 0.02 mm for pulsed laser cutting. For comparable cutting with a continuous power laser, a heat-affected zone between 0.05 and 0.10 mm is predicted. Thermal stress levels were predicted to be an order of magnitude lower for pulsed laser cutting than for continuous power cutting. The stress levels predicted by the model also increased with cut speed. Experimentally, pulsed laser cutting yielded better cut quality, based on less cracking, than continuous power cutting. In addition, the cut quality deteriorated as the cutting speed was increased for the continuous power laser. Presently, application of pulsed laser cutting is limited by its low cutting speed, which is restricted by the energy density of the laser. The model predicts that increasing energy density will decrease the size of the heat-affected zone and increase the maximum cutting speed. Therefore, pulsed laser cutting at high speeds should be attainable without deterioration in cut quality. / Master of Science

We demonstrate four experimentally simple methods for measuring very complex ultrashort light pulses. Although each method is comprised of only a few optical elements, they permit the measurement of extremely complex pulses with time-bandwidth products greater than 65,000. First, we demonstrate an extremely simple frequency-resolved-optical gating (GRENOUILLE) device for measuring the intensity and phase of pulses up to ~20ps in length. In order to achieve the required high spectral resolution and large temporal range, it uses a few-cm-thick second harmonic-generation crystal in the shape of a pentagon. This has the additional advantage of reducing the device's total number of components to three. Secondly, we introduce a variation of spectral interferometry (SI) using a virtually imaged phased array and grating spectrometer for measuring long complex ultrashort pulses up to 80 ps in length. Next, we introduce a SI technique for measuring the complete intensity and phase of relatively long and very complex ultrashort pulses. It involves making multiple measurements using SI (in its SEA TADPOLE variation) at numerous delays, measuring many temporal pulselets within the pulse, and concatenating the resulting pulselets. Its spectral resolution is the inverse delay range--many times higher than that of the spectrometer used. The waveforms were measured with ~ fs temporal resolution over a temporal range of ~ns and had time-bandwidth products exceeding 65,000, which to our knowledge is the largest time-bandwidth product ever measured with ~fs temporal resolution. Finally, we demonstrate a single-shot measurement technique that temporally interleaves hundreds of measurements with ~fs temporal resolution. It is another variation of SI for measuring the complete intensity and phase of relatively long and complex ultrashort pulses in a single shot. It uses a grating to introduce a transverse time delay into a reference pulse which gates the unknown pulse by interfering it at the image plane of an imaging spectrometer. It provided ~125 fs temporal resolution and a temporal range of 70 ps using a low-resolution spectrometer.

Die Messung ultrakurzer Laserpulse ist ein Eckpfeiler der ultraschnellen Laserphysik, da die Gültigkeit eines Experiments von der Glaubwürdigkeit seiner Messtechnik abhängt. Etablierte Puls-Charakterisierungstechniken beruhen jedoch häufig auf einer Mittelung über viele Pulse. Daher können sie falsche Informationen liefern, wenn die zeitliche Form von Puls zu Puls variiert. Diese Dissertation bietet Strategien zum sicheren Erfassen und Messen einer Degradierung der Puls-Kohärenz mit Hilfe von frequenzaufgelöstem optischem Gating (FROG), spektraler Phaseninterferometrie für die direkte Rekonstruktion elektrischer Felder (SPIDER) und Dispersionsscan (D-scan). Zu diesem Zweck werden Verbesserungen der Charakterisierungstechniken entwickelt. Die in dieser Arbeit entwickelten neuen Werkzeuge eröffnen nun einen Weg zur Untersuchung der Degradierung der Inter-Puls-Kohärenz, was eine zuverlässige Ultrakurzpulsmetrologie ermöglicht und das zuvor nicht nachweisbare Problem der Pulsfolgeninstabilität löst. / The measurement of ultrashort laser pulses is a cornerstone of ultrafast laser physics, as the validity of any experiment depends on the credibility of its measurement technique. However, established pulse characterization techniques often rely on averaging over many pulses. Therefore, they can return incorrect information if the temporal shape varies from pulse to pulse. This thesis provides strategies to safely detect and measure interpulse coherence degradation, using frequency-resolved optical gating (FROG), spectral phase interferometry for direct electric-field reconstruction (SPIDER), and dispersion scan (d-scan). To this end, improvements of the characterization techniques themselves are devised. The set of new tools developed in this thesis now opens up an avenue for the investigation of interpulse coherence degradation, leading to a more reliable ultrashort pulse metrology and solving the previously undetectable problem of pulse train instability.

Thesis (PhD)--Stellenbosch University, 2015. / ENGLISH ABSTRACT: In this work we investigate a new method to measure the electric field of ultrafast laser pulses by extending a known measurement technique, ptychography, in the spatial domain to the time domain which we call time domain ptychography. The technique requires the measurement of intensity spectra at different time delays of an unknown temporal object and a known probe pulse. We show for the first time by measurement and calculation that this technique can be applied with excellent results to recover both the amplitude and phase of a temporal object. This technique has several advantages, such as fast convergence, the resolution is limited by the usable measured spectral bandwidth and the recovered phase has no sign ambiguity. We then extend the technique to pulse characterization where the probe is derived form the temporal object by filtering meaning the probe pulse is also unknown, but the spectrum of the probe pulse must be the same as the spectrum of the temporal object before filtering. We modify the reconstruction algorithm, now called ptychographic iterative reconstruction algorithm for time domain pulses (PIRANA), in order to also reconstruct the probe and we show for the first time that temporal objects, a.k.a laser pulses, can be reconstructed with this new modality. / AFRIKAANSE OPSOMMING: In hierdie werk het ons ’n nuwe metode ondersoek om die elektriese veld van ’n ultravinnige laser puls te meet deur ’n bekende meettegniek wat gebruik word in die ruimtelike gebied, tigografie, aan te pas vir gebruik in die tyd gebied genaamd tyd gebied tigografie. Die tegniek vereis die meting van ’n reeks intensiteit spektra by verskillende tyd intervalle van ’n onbekende ‘tyd voorwerp’ en ’n bekende monster puls. Ons wys vir die eerste keer deur meting en numeriese berekening dat hierdie tegniek toegepas kan word met uitstekende resultate, om die amplitude en fase van ’n ‘tyd voorwerp’ te meet. Hierdie tegniek het verskeie voordele, die iteratiewe proses is vinnig, die resolusie van die tegniek word bepaal deur die spektrale bandwydte gemeet en die fase van die ‘tyd voorwerp’ word met die korrekte teken gerekonstrueer. Ons het hierdie tegniek uitgebrei na puls karakterisering waar die monster pulse afgelei word, deur ’n bekende filter te gebruik, van die onbekende ‘tyd voorwerp’ nl. die inset puls. Ons het die iteratiewe algoritme wat die ‘tyd voorwerp’ rekonstrueer aangepas om ook die monster puls te vind en ons wys dat ons hierdie metode suksesvol kan gebruik om laser pulse te karakteriseer

Thesis (MSc (Physics))--University of Stellenbosch, 2010. / ENGLISH ABSTRACT: This thesis consists of two parts; the first part is a discussion on the detailed history of the development of different types of modelocked lasers, especially the neodymium-doped lasers. The second part describes the design and development of a modelocked diode-end-pumped solid state Nd:YVO4 laser using Semiconductor Saturable Absorbers. The first part of this work will cover the history of modelocking where different types of lasers were used to generate ultrashort pulses. The discussion will mainly focus on neodymium-doped lasers such as Nd:YVO4, where we will look at the spectral properties such as energy levels, absorption and emission wavelengths of such a laser. The discussion will also look at different types of optical pump sources; such as diode lasers and flashlamps, where we will see the advantages of using diode lasers as pump sources due to their better operating conditions and efficiency. We will also look at two different types of diode pumping setup schemes, which are end-pumping and side pumping; where we will discover that diode-end-pumping is a better scheme for laser mode matching resulting in high efficiency and very good beam quality when compared to side pumping. The gain bandwidth of the laser material will also be discussed showing that a laser material with a very large gain bandwidth and broad emission bandwidth is suitable for generating ultrashort pulses, such as Ti:Sapphire crystal. The discussion will also cover ultrafast lasers that have a small amplification bandwidth suitable for diode-end-pumping and that produce high average output power. Ultrafast lasers with low amplification bandwidth such as Nd:YAG and Nd:YVO4 will be discussed showing that they can generate very short pulses with durations of down to 19 ps and 20 ps respectively and average output powers of 27 W and 20 W. The technique of creating ultrashort pulses which is called modelocking will be discussed, where passive modelocking will be shown to be more suitable for creating ultra short pulses in the femtosecond region and active modelocking in the picosecond region. The discussion will also cover saturable absorbers for passive modelocking where we will discuss the use of semiconductor saturable absorber mirrors to generate reliable self starting modelocked pulses. We will also cover the instabilities associated with using saturable absorbers where we will discuss different methods for reducing the instabilities by using gain media with the smallest saturation fluence. The second part of the work will deal with the design and development of SESAM modelocked diode-end-pumped Nd:YVO4 lasers. This part will include a discussion on the resonator design criteria’s for achieving a stable modelocked diode-end-pumped solid-state laser. The choice of using Nd:YVO4 as a gain medium will be shown to be influenced by its large cross sectional area, which is useful in increasing the gain bandwidth for possible ultrashort pulse generation. The resonator for high power continuous wave (cw) output has been designed using simulation software developed at St Andrews University. We will also discuss stability criteria such as the laser spot size inside the crystal and on the end mirror and how they can be incorporated into the resonator design software. The discussion will also include the pump setup design and the efficient cooling method of the crystal using a copper heat sink. The methodology of obtaining stable, thermal lens invariant, single transverse mode operation during power scaling of Nd:YVO4 lasers will be discussed. A lens relay approach is used to extend the cavity length so as to introduce spot size control in the designed diode-end-pumped Nd:YVO4 laser that will be shown to produce a maximum average output power of 10.5 W with an average beam quality factor M2 of 1.5. We will also discuss the incorporation of a single quantum well SESAM within the extended diode-end-pumped Nd:YVO4 laser resulting in cw-modelocked pulses at an average output power of 2.8 W with pulse repetition frequency of 179 MHz, equivalent to the cavity round trip time of 5.6 ns. The incorporation of the double quantum well SESAM will also be shown to produce stable Q-switched modelocked pulses at an average output power of 2.7 W with pulse repetition frequency of 208 KHz. / AFRIKAANSE OPSOMMING : Hierdie tesis bestaan uit twee dele. Deel 1 is ‘n indiepte bespreking rondom die ontwikkelingsgeskiedenis van Modusgebonde lasers, veral van Neodemiumdoteerde lasers. Deel 2 beskryf die ontwerp en ontwikkeling van ‘n Modusgebonde diodeentgepompde vastetoestand Nd:YVO4 laser deur van ‘n Halfgeleier Versadigbare Absorbeerder (SESAM) gebruik te maak. Die eerste afdeling fokus op Modusbinding om ultrakort pulse te ontwikkel in verskillende tipes lasers. Die bespreking sentreer rondom Neodemiumdoteerde lasers soos Nd:YVO4. In hierdie geval beskou ons ook die spektraaleienskappe van die laser vir beide die absorpsie en emissie golflengtes. Verder word verkillende tipes pompbronne ondersoek (soos diodelasers en flitslampe). Die voordele van diodelasers kom sterk na vore a.g.v. beter werking en effektiwiteit. Verskillende pompopstellings word ook ondersoek naamlik ent-en kantpomping. Entpomping kom hier na vore as die beter opsie i.t.v. laser-moduspassing. Dit lei tot ‘n hoër effektiwiteit wat ‘n beter straalkwaliteit tot gevolg het, in vergelyking met kantgepompde opstellings. Die versterkingsbandwydte word ook bespreek: ‘n groot versterkingsbandwydte en breë emissiebandwydte is geskik om ultrakort pulse te ontwikkel. Ti:Saffier is ‘n goeie voorbeeld. Ultravinnige lasers met ‘n klein versterkingsbandwydte word ook bespreek aangesien dit geskik is vir diodeentpomping wat dan ‘n hoë gemiddelde uitsetdrywing lewer. Nd:YAG en Nd:YVO4 word ondersoek en daar word getoon dat hul pulse van so kort as 19 ps en 20 ps onderskeidelik teen ‘n gemiddelde uitsetdrywing van 27 W en 20 W kan lewer. Die tegniek waarmee ultrakort pulse geskep word is Modusbinding: passiewe modusbinding is meer geskik vir femtosekonde pulse en aktiewe modusbinding is meer geskik vir pikosekonde pulse. Verder word versadigbare absorbeerders bespreek, vir hul gebruik in die betroubare selfinisiërende modusgebonde pulse. Die onstabiliteite geassosieer met versadigbare absorbeerders word ook bespreek asook verskillende metodes om dit te minimaliseer. Die tweede afdeling behandel die ontwerp en ontwikkeling van ‘n SESAM modusgebonde diode-entgepompde Nd:YVO4 laser. Die resonator ontwerpspesifikasies vir stabiele werking word ook bespreek. Die keuse van Nd:YVO4 as versterkingsmedium is a.g.v. die groot deursnitarea wat die versterkingsbandwydte verhoog, om ultrakort pulse te genereer. Die resonator vir hoë drywing kontinuestraal werking is ontwerp deur van St Andrews sagteware gebruik te maak. ‘n Bespreking van stabiliteitsspesifikasies soos die laser kolgrootte, binne die kristal asook op die entspieël volg, asook die pompmetodiek en effektiewe verkoeling van die kristal. Die totale metodiek rondom die verkryging van ‘n stabiele, termieselens invariante, enkele transversale modus laser word bespreek met die oog op drywingsverhoging. Die geval onder bespreking is waar die laser se kolgrootte beheer kan word op die entspieël deur die resonatorlengte aan te pas. Dit word getoon dat dit ‘n kontinuestraal laser van 10.5 W drywing kan lewer teen die maksimum gemiddelde straalkwaliteit van M2 = 1.5. Die byvoeging van ‘n enkele kwantumput SESAM in die laser het modusgebonde pulse tot gevolg. Die gemete waardes was 2.8 W gemiddelde drywing met ‘n pulsherhalingstempo van 179 MHz wat in lyn is met die pulsbewegingstyd in die resonator van 5.6 ns. Deur van ‘n dubbele kwantumput SESAM gebruik te maak word Q-geskakelde modusgebonde pulse verkry, teen ‘n gemiddelde uitsetdrywing van 2.7 W en ‘n pulsherhalingstempo van 208 KHz.

Thesis (MSc)--Stellenbosch University, 2013. / ENGLISH ABSTRACT: An ultra-fast laser pulse can be described in the time or frequency domain. If the timebandwidth product of an ultra-fast pulse is not satisfied, then the pulse is stretched. Stretching can be described in the time or frequency domain. In the time domain, it is called a chirp and in the frequency domain, it is known as the group delay dispersion GDD. Various techniques can be used to stretch and compress laser pulses. In this project, a prism pulse compressor used for compressing stretched pulses was built. A 200nm supercontinnum generated in an all normal dispersion photonic crystal fibre (ANDi-PCF) was compressed using the prism pulse compressor from 2ps to 140fs. The experiment and physical interpretation presented in this project suggest that a shorter pulse duration less than the measured 140fs of the compressed supercontinnum can be obtained. / AFRIKAANSE OPSOMMING: 'n Ultra-vinnige laser puls kan beskryf word in tyd of frekwensie. As die tyd-bandwydte produk van ‘n ultra-vinnige puls nie bevredig is nie, dan is die puls uitgerek. Hierdie uitrekking kan beskryf word in tyd of frekwensie. In tyd word dit tjirp genoem en in frekwensie groep vertraging dispersie. Verskeie tegnieke kan gebruik word om ‘n laser puls te rek of saam te pers. In hierdie projek is ‘n prisma puls kompressor gebou om uitgerekte pulse saam te pers. ‘n 200nm bre e bandwydte puls (“supercontinuum”) is gegenereer in ‘n fotoniese kristal optiese vesel wat uitsluitlik normale dispersie toon (ANDI-PCF) en die puls is toe saamgepers met behulp van die prisma puls kompressor van‘n oorspronklike 2ps na 140fs. Die eksperiment en fisiese interpretasie wat in hierdie projek aangebied word dui daarop dat ‘n nog korter puls, minder as die gemete 140 fs, verkry kan word deur die bre e bandwydte puls verder saam te pers.

In this thesis, novel regimes of mode locking in quantum dot semiconductor laser diodes have been investigated by exploiting the unique features offered by quantum dots. Using an unconventional approach, the role of excited state transitions in the quantum dots was exploited as an additional degree of freedom for the mode locking of experimental quantum dot lasers. For the first time, passive mode locking via ground (1260nm) or excited state (1190nm) was demonstrated in a quantum dot laser. Picosecond pulses were generated at a repetition rate of 21GHz and 20.5GHz, for the ground and excited states respectively, with average powers in excess of 25mW. Switching between these two states in the mode-locking regime was achieved by changing the electrical biasing conditions, thus providing full control of the operating spectral band. A novel regime for mode locking in a quantum-dot laser was also investigated, where the simultaneous presence of cw emission in the excited-state band at high injection current levels, dramatically reduced the duration of the pulses generated via the ground state, whilst simultaneously boosting its peak power. This represents a radically different trend from the one typically observed in mode-locked lasers. From this investigation, it was concluded that the role of the excited state can not be neglected in the generation of ultrashort pulses from quantum-dot lasers. Stable passive mode locking of a quantum-dot laser over an extended temperature range (from 20ºC to 80ºC) was also demonstrated at relatively high output average powers. It was observed that the pulse duration and the spectral width decreased significantly as the temperature was increased up to 70ºC. The process of carrier escape in the absorber was identified as the main contributing factor that led to a decrease in the absorber recovery time as a function of increasing temperature which facilitated a decrease in the pulse durations. These results are shown to open the way for the ultimate deployment of ultra stable and uncooled mode-locked semiconductor diode lasers.