Práce popisuje investiční prostředí a investiční klima v Ruské federaci, a tím poskytuje podstatné informace o možnostech a případných příležitostech investorům. Práce je rozdělena na část teoretickou a praktickou. Teoretická část se věnuje základním teoretickým aspektům tak, aby čtenář lepe vnímal praktickou část. Popsán aktuální stav ekonomického prostředí a všechny pozitivní a negativní stránky, vyplývající z něho, pro vkladatele. Popsána jsou i některá prostředí, ve kterém se zahraniční investoři mohou uplatnit. Závěrečná část práce představuje SWOT analýzu, a jsou zde shrnuty všechny poznámky a připomínky pro investora

Oceňování aktiv představuje klíčový problém v účetnictví, protože způsob ocenění majetku a závazků přímo ovlivňuje výši vykazovaných aktiv a pasiv a má vliv na výši nákladů podnikání i na velikost vykazovaného výsledku hospodaření. Provedené analýzy potvrzují, že metody, které firma používá pro oceňování svého majetku, mají vliv na vypovídací schopnosti účetních výkazů. Použití metody odpisování dlouhodobého majetku ovlivňují zatížení nákladů v jednotlivých letech a tím i výši hospodářského výsledku v časové řadě. Stejný vliv se projevuje i na způsobu účtování zásob nebo tvorbě a uplatňování opravných položek.

Vstupem České republiky do Evropské unie byla přijata Společná zemědělská politika EU (SZP). Cílem diplomové práce je zjistit a posoudit smysl zemědělských dotací pro malé soukromé zemědělce a jejich možnosti získání. V prvních kapitolách práce je SZP popsána obecně. Třetí a čtvrtá kapitola se zabývá Českou republikou a jejím vývojem v zemědělském sektoru. Pátá kapitola je věnována novému rozpočtovacímu období 2007 ? 2013 a především Programu rozvoje venkova. Poslední tři kapitoly jsou věnovány hypotetickému příkladu soukromého zemědělce, jeho možnostem při získávání dotací, kontrolám a kalkulací příjmů a nákladů při zemědělské činnosti.

A doença de Chagas, também chamada de tripanossomíase americana é a terceira doença parasítica mais presente no mundo, perdendo apenas para malária e esquistossomose. As terapias existentes atualmente para essa tripanossomíase são insatisfatórias e pouca atenção tem sido dada para o desenvolvimento de novos fármacos. Os medicamentos utilizados atualmente apresentam boa atividade apenas na fase inicial da doença e geram efeitos colaterais severos nos pacientes. <br /> As hidrazonas representam uma classe de compostos imínicos de grande versatilidade estrutural e importante atividade biológica em diversos níveis, sendo observados resultados de atividade tripanocida interessantes de hidrazonas coordenadas a rutênio. Por outro lado, complexos com oxovanádio coordenado a derivados de quinoxalinas apresentam melhores atividades do que os ligantes na forma livre e que complexos formados por essas quinoxalinas com outros metais. <br /> Esse trabalho buscou unir as propriedades biológicas das hidrazonas e vanádio de forma a obter complexos com boa atividade tripanocida. Foram sintetizados dois ligantes hidrazonas derivados da 2-tiofenofenohidrazida, e através dos mesmos foram desenvolvidos dez novos complexos de oxovanádio (V). Os produtos foram caracterizados por ponto de fusão, análise elementar, espectroscopia na região do infravermelho e do UV-Vis., ressonância magnética nuclear (RMN 1H) e difração de raios X em monocristal, sendo obtidos três classes de complexos. Duas classe são formadas por complexos com ligantes mistos, na forma [VO(L)(OR)] com R=metil, etil, n-propil e L=hidrazona, e na forma [VO(L)(mal)] com L=hidrazona e mal= pirona maltol. Outra classe obtida é formada por binucleares de oxovanádio na forma [(VOL)2(&mu;-O)] com L=hidrazona e os centros de vanádio ligados por uma ponte &mu;-oxo. As estruturas obtidas para os complexos mistos com os alcóxidos e para os dímeros apresentam geometria piramidal quadrática distorcida, enquanto que os complexos mistos com maltol apresentam geométrica octaédrica distorcida. <br /> Ensaios in vitro contra cepas de T. cruzi mostraram resultados interessantes (SI iguais ou maiores que 10) para que continue a exploração dos tipos de complexos formados e novos ensaios biológicos devem ser realizados para verificar o mecanismo de ação e a atividade in vivo desses compostos, com intuito de obter um novo fármaco antichagásico baseado em vanádio no futuro. / Chagas disease, also called American trypanosomiasis is the third most present parasitic disease in the world. The existing therapies for this trypanosomiasis are unsatisfactory and a little attention has been given to the development of new drugs. The drugs currently used exhibit good activity only in the early phase of the disease and generate severe side effects in patients. <br /> Hydrazones represent a class of iminic compounds with good structural variability and important biological activity at various levels, and interesting results of trypanocidal activity are observed for hydrazones coordinated to ruthenium. On the other hand, complexes with oxovanadium coordinated to quinoxaline derivatives have better activities than the free ligands and complexes with other metals coordinated to that quinoxalines. <br /> This study aimed to unite the biological properties of hydrazones and vanadium to obtain complexes with good trypanocidal activity. Two hydrazones ligands derived from 2- tiofenofenohidrazida were synthesized, and through that ligands, ten new complexes of oxovanadium (V) were developed. The products were characterized by melting point, elemental analysis, infrared and UV - Vis. spectroscopies, nuclear magnetic resonance (1H NMR) and single crystals x-ray diffraction. Three classes of complexes were obtained. Two of them are formed by complexes with mixed ligands, one at [VO(L)(OR) ] form, with R = methyl , ethyl , n- propyl and L = hydrazone , and the other one with the [VO(L)(mal)] empirical form, with L = hydrazone and mal = pyrone maltol. Another class consists of binuclear oxovanadium complexes obtained at [(VOL)2(&mu;-O)] form, with L = hydrazone and the vanadium centers connected by a &mu;-oxo bridge. The structures obtained for the mixed complexes with the alkoxides and dimers have quadratic distorted pyramidal geometry, while the mixed maltol complexes have distorted octahedral geometry. <br /> In vitro assays against T. cruzi strains showed interesting results (SI equal to or greater than 10) to continue the exploration of the types of complexes formed and new biological tests must be conducted to verify the mechanism of action and in vivo activity of these compounds, in order to obtain a new vanadium-based antichagasic drug in the future.

My thesis deals with lack of self-confidence and lack of freedom in acting that comes with it. It tries to search and denominate single aspects which can lead to stated issue or eventually to its intensifying. Furthermore it’s a subjective reflection of my entire studies at Theatre Faculty since my first year until graduation productions at Disk theatre

During recent years the thickness of the gate oxide has been reduced considerably. The progressive miniaturization of devices has caused several phenomena to emerge such as quasi-breakdown, direct tunneling and stress induced leakage currents. Such phenomena significantly modify the performance of the scaled-down MOSFETs. As a part of this research work, an effort has been made to study the performance and characteristics of the thin Gate oxide for MOSFETs and Tunnel Oxide for Floating Gate (FG) MOS devices. The exponential dependence of tunnel current on the oxide-electric field causes some critical problems in process control. A very good process control is therefore required. This can be achieved by finding out the exact value of F-N tunneling parameter. This research work also is an effort of finding an accurate value for parameter B and its dependence on the oxide thickness as the device are scaled down to a level where the probability of Direct Tunneling mechanism gains more prominence. A fully automated Low Current Measurement workstation with noise tolerance as low as 10-15 A was set up as a part of this research. C-V and I-V curves were analyzed to extract, determine and investigate the oxide thickness dependence of F-N parameter B. For oxide thickness in the range10~13 nm, the parameter B ranged between 260 and 267. Thus it can be said that it is not sensitive to the change in oxide thickness in this range. However it was noticed that for thickness around 7nm wide variety of results were obtained for the Fowler-Nordheim parameter B (B ranged from 260 to 454). This can be attributed to the enhancement in the leakage current due to the direct tunneling. Hence to have tight control over VT for a NVM, new algorithms need to be developed for even better process control for oxide thickness in the range of 7 nm and below.

Este trabalho teve como objetivo a investigação de novos materiais promissores para a supercondutividade presentes no sistema ternário do Zr-V-Ga. Os resultados apresentados de magnetização, resistividade e calor específico, mostram de forma inequívoca que o Zr3V2Ga4 é um novo material supercondutor com temperatura crítica de transição supercondutora nas proximidades de 14,3K. O estudo do composto ZrV2Ga4 mostrou que a supercondutividade é revelada com temperatura crítica de aproximadamente 14,2 K. Medidas de calor especifico realizadas nessas amostras evidenciam de forma clara manifestações de supercondutores multibandas. Outra investigação importante realizada foi a substituição parcial de vanádio por titânio na estequiometria do composto ZrV2Ga4 representado pela composição global ZrV2-xTixGa4, revela que a substituição parcial de vanádio por titânio suprime a supercondutividade neste composto. Isto é mais evidente quando a substituição total de V por Ti suprime totalmente o comportamento supercondutor. Estes resultados sugerem que a supercondutividade pode ser dependente da unidade aniônica [V2Ga4]4-. Finalmente, esta dissertação mostra que o sistema ternário Zr-V-Ga é bastante rico em fases supercondutoras ainda não reportadas na literatura. / This work aimed to investigate new promising materials for superconductivity present in the ternary system of Zr-V-Ga. The presented results of magnetization, resistivity and specific heat, show unequivocally that the Zr3V2Ga4 is a new superconducting material with critical temperature of superconducting transition close to 14.3K. The study of compound ZrV2Ga4 showed that superconductivity is revealed at a critical temperature of approximately 14.2K. Specific heat measurements carried out on these samples clearly demonstrate manifestations of multiband superconductors. Another important investigation carried out was the partial substitution of vanadium by titanium in the stoichiometry of the ZrV2Ga4 compound represented by the global composition ZrV2-xTixGa4, reveals that the partial substitution of vanadium by titanium suppresses the superconductivity in this compound. This is most evident when the total substitution of V for Ti totally suppresses the superconducting behavior. These results suggest that superconductivity may be dependent on the anionic unit [V2Ga4]4-. The results presented in this dissertation reveal that the ternary system Zr-V-Ga is rich in new superconducting materials not reported yet.

Die vakuoläre Protonen-ATPase, kurz V-ATPase, ist ein multimerer Enzymkomplex, der in fast jeder eukaryotischen Zelle zu finden ist und den aktiven elektrogenen Transport von Protonen über Membranen katalysiert. Die Aktivität der V-ATPase ist essentiell für eine Vielzahl physiologischer Prozesse. Ein grundlegender Mechanismus zur Regulation der V-ATPase-Aktivität ist die reversible Dissoziation des Holoenzyms in den integralen VO-Komplex, der als Protonenkanal dient, und den cytosolischen V1-Komplex, der ATP hydrolysiert und somit den Protonentransport energetisiert. Die Untereinheit C, die im dissoziierten Zustand der V-ATPase als einzige Untereinheit isoliert im Cytoplasma vorliegt, scheint bei der Bildung des aktiven Holoenzyms eine Schlüsselrolle zu übernehmen. In den Speicheldrüsen der Schmeißfliege Calliphora vicina ist die V-ATPase an der Speichelsekretion beteiligt. In den sekretorischen Zellen wird die Bildung des V-ATPase-Holoenzyms in der apikalen Plasmamembran durch das Neurohormon Serotonin (5-HT) stimuliert. Der Effekt von 5-HT auf die V-ATPase wird intrazellulär durch die Proteinkinase A (PKA) vermittelt und hält nur für die Dauer der Stimulierung an. In der vorliegenden Arbeit wurde mittels Phosphoproteinfärbungen und 2D-Elektrophorese nachgewiesen, dass infolge einer Stimulierung der Drüsenzellen mit 5-HT die Untereinheit C der V-ATPase durch die PKA reversibel phosphoryliert wird. Die Phosphorylierung geht einher mit einer Umverteilung der Untereinheit C aus dem Cytoplasma zur apikalen Plasmamembran und der Bildung des aktiven Holoenzyms. Immuncytochemische Untersuchungen zeigten, dass die katalytische Untereinheit der PKA ebenfalls umverteilt wird und in stimulierten Zellen im Bereich der apikalen Plasmamembran konzentriert vorliegt. Um herauszufinden welche Proteinphosphatase der PKA entgegenwirkt, wurden luminale pH-Messungen durchgeführt und der Effekt von spezifischen Proteinphosphatase-Inhibitoren und veresterten Komplexbildnern zweiwertiger Kationen auf die V-ATPase-Aktivität untersucht. Diese Messungen führten zu der Schlussfolgerung, dass eine Proteinphosphatase des Typs 2C an der Inaktivierung der V-ATPase beteiligt ist. Mit weiteren Phosphoproteinfärbungen konnte gezeigt werden, dass die Dephosphorylierung der Untereinheit C ebenfalls durch eine Proteinphosphatase 2C katalysiert wird und dies vermutlich die Dissoziation des VO- und V1-Komplexes begünstigt. Darüber hinaus konnte durch luminale pH-Messungen und ergänzende biochemische Untersuchungen eine Calcineurin-vermittelte Modulation des cAMP/PKA-Signalweges durch den parallel aktivierten IP3/Ca2+-Signalweg und damit einhergehend eine Beeinflussung der V-ATPase-Aktivität durch den [Ca2+]-Spiegel nachgewiesen werden. / The vacuolar-type H+-ATPase (V-ATPase) is a multimeric enzyme that can be found in nearly every eukaryotic cell. It catalyses the active electrogenic transport of protons across membranes and is essential for a multitude of physiological processes. A fundamental mechanism to regulate V-ATPase activity is the reversible dissociation of the holoenzyme into an integral proton conducting VO-complex and a cytosolic V1-complex that hydrolyses ATP and thus energises proton translocation. Subunit C occurs isolated in the cytoplasm upon dissociation of the V-ATPase complexes and seems to be critical for the formation of active holoenzymes. In the salivary glands of the blowfly Calliphora vicina the V-ATPase is involved in fluid secretion. In secretory cells, formation of the V-ATPase holoenzyme is stimulated by the hormone serotonin (5-HT). The effect of 5-HT on V-ATPase activity is mediated by protein kinase A (PKA) and persists for the duration of the 5-HT stimulus. In this study, it was shown by phosphoprotein stainings and two-dimensional electrophoresis that subunit C of the V-ATPase becomes phosphorylated by PKA upon exposure of blowfly salivary glands to 5-HT. Parallel to the phosphorylation event, subunit C translocates from the cytoplasm to the apical plasma membrane for the assembly of active V-ATPase holoenzymes. Using immunofluorescence staining, it could be shown that PKA catalytic subunit translocates as well to the apical membrane upon 5-HT stimulation. To examine which protein phosphatase counteracts PKA, luminal pH-measurements were carried out. Based on the results with protein phosphatase inhibitors and esterified chelating agents of bivalent cations, it may be concluded that a protein phosphatase 2C is involved in the process leading to V-ATPase inactivation. Phosphoprotein stainings revealed that dephosphorylation of subunit C is likewise catalysed by a protein phosphatase 2C. Therefore the dephosphorylation of subunit C seems to promote dissociation of VO- and V1-complexes. Finally, luminal pH-measurements and supplemental biochemical experiments revealed a Ca2+/calcineurin-mediated modulation of the cAMP/PKA signalling cascade and an influence of intracellular calcium on the V-ATPase activity.

Scania är en av världens största tillverkare av lastbilar, bussar och marina motorer och examensarbetet har utförts i syfte att utveckla en ny remmätrigg för att bättre kunna verifiera slitage, livslängd och töjning efter olika testcykler på Poly-V remmar. Riggen skall utföra mätningar enligt standard SAE-J1459 och klara att mäta remlängder mellan 1000-3300 mm. Därtill måste konstruktionen vara utformad enligt gällande säkerhetsföreskrifter och ergonomiska aspekter beaktas. Mätningar av Poly-V remmar sker efter principen att remmen spänns in mellan två remskivor med en kraft på 100 N per ribba. Vid examensarbetets start fanns en äldre mätrigg att tillgå, vilken inte längre är i bruk p.g.a. att den inte uppfyller alla krav för mätprocessen. Den har dock använts i syfte för att identifiera ergonomi- och säkerhetsrisker. Påvisade risker har sedan beaktats vid utformningen av koncept, för att begränsa dessa vid konstruktion av den nya mätriggen. Konstruktionen av den nya remmätriggen är framtagen med hjälp av erkänd produktutvecklingsmetodik, med arbete från idé till färdig produkt. Allt i enlighet med en förstudie i konstruktionsmetodik, en konceptstudie som behandlar ett flertal koncept, konceptval och analys av teknisk utrustning som används i den nya konstruktionen samt konstruktionsarbete och utvärdering. Vid examensarbetets slut har en färdigmonterad remmätrigg levererats klar för användning. Konstruktionen klarar att mäta remlängder mellan 1000-3300 mm och upp till tolv ribbor. Mätprocessen enligt SAE-J1459 kan utföras samtidigt som riggen är utformat med ergonomi och säkerhet i åtanke. Tillhörande remmätriggen finns en användarmanual som beskriver rutiner kring användning av utrustningen, risker och själva mätprocessen steg för steg. I rapporten bilagas en förstudie inom ergonomi och säkerhet, som har utförts vid examensarbetets första fas med syfte att användas som grund vid utformningen av den nya riggen. Det för garantera att hänsyn är tagen till gällande ergonomi- &amp; arbetsmiljö-föreskrifter. / Scania is one of the World greatest producers of trucks, busses and marine engines. The purpose of the master thesis has been to develop a new measuring rig for Poly-V belts, to better be able to verify wear, duration and elongation at different testing cycles. The rig shall do measurements following the SAE-J1459 standard and be able to perform on belts with length between 1000-3300 mm. In addition, the design must be made in accordance with safety regulations and ergonomic aspects. Measurement of Poly-V belts are carried out by straining the belt between to pulleys with a force of 100 N per crossbar. At the project start access was granted to an old measuring rig, which is no longer in use due to not fulfilling the requirements for the measuring procedure. However, it has been used to identify ergonomic and safety gaps. The development of new concepts has then been carried out with those gaps in mind. The design of the new measuring rig is developed by using proven product development methodology, which covers work from idée to completed product. That includes accordance with a pre-study in design methodology, a concept study which deals with a number of concepts, the selection of concepts and an analysis of the technical equipment used in the new design during the product design. The result of the thesis is a completed rig, delivered and ready to use. The rig is capable of measuring ribbed belt length between 1000-3300 mm and up to twelve ribs. Measurement process according to SAE-J1459 can be performed meanwhile the rig is designed with ergonomics and safety aspects. A user manual describing the use of equipment, risks and the actual measurement process step by step is also included with the rig. The report annexes a pre-study of ergonomic and safety, which has been written in the initial phase of the thesis. The pre-study has been used as a guide for the design of the new rig, taking into consideration the existing regulations from different acquis.

In recent years, the Bantu object affix that is commonly known as the object marker (OM) has attracted considerable debate regarding its status in generative grammar. One view takes the OM to be an incorporated pronoun (see for example, Bresnan and Mchombo 1987; Bresnan 1993). Their analysis is based mainly on the contrast between object marking and subject marking. The subject marker (SM), they point out, behaves as an agreement marker while the object marker behaves like a bound pronoun, freeing the noun from word order restrictions, permitting contrastive focus like a bound pronoun, and permitting non-local anaphoric relations. The other view takes OM to be an agreement marker which licenses a null object (see for example, Bergvall 1987; Kinyalolo 1991; Ngonyani 1996). In this paper I take the second position and, on the basis of Kiswahili constructions in which the lexical object is not realized, I argue that a null object analysis is consistent with VP ellipsis facts, idiom chunks, and co-occurrence between OM and the lexical object. It is consistent with the general analysis of agreement as instantiation of Spec-Head relation (Chomsky 1986a, Kinyalolo 1991). I demonstrate using the elliptical constructions that the verb moves to an Inf-position.