Orientador: José Raimundo de Oliveira / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Elétrica e de Computação / Made available in DSpace on 2018-08-20T00:16:51Z (GMT). No. of bitstreams: 1 Belle_Adriane_M.pdf: 2685338 bytes, checksum: 49d8e047de984e5ce1da6b33a9aac90c (MD5) Previous issue date: 2011 / Resumo: A tecnologia dos processadores tem crescido rapidamente nos últimos anos. Em contrapartida, o ensino de arquitetura de computadores tem dificuldade de acompanhar esta evolução. Os livros textos e as aulas ainda utilizam de recursos estáticos que precisam de longas explicações. Esta dinâmica torna-se incompatível, inclusive, com as experiências, como usuários, de muitos alunos. Este trabalho propõe o desenvolvimento de um ambiente computacional (framework) para apoio ao ensino de arquitetura de processamento, denominado MODPRO. A ideia é dispor de módulos que possam ser interligados formando diversas possíveis estruturas de processamento. Desta maneira, o professor pode desenvolver junto aos alunos e expor de forma visual (utilizando de animações) desde componentes básicos até estruturas de processamento mais avançadas. O MODPRO é composto por um simulador, denominado SIMPRO o qual exibe de forma animada, passo a passo, ou em tempo real, o fluxo de dados e de sinais dentro da estrutura de processamento estudada. O SIMPRO foi desenvolvido em linguagem Javascript e utiliza recursos de Cascading Style Sheets, podendo, ainda, ser acessado pela web. O MODPRO ainda é composto por um emulador, chamado EMUPRO que contém os mesmos módulos do SIMPRO. O seu diferencial está relacionado ao fato de ter sido totalmente desenvolvido em hardware, utilizando a ferramenta QUARTUS II da Altera. Com este recurso, os alunos podem, em laboratório, validar a estrutura desenvolvida em classe. Por serem modulares, tanto o simulador SIMPRO quanto o emulador EMUPRO permitem que novos recursos (módulos) possam ser adicionados, permitindo assim, o ensino e o estudo de diferentes estruturas de processamento / Abstract: The processor technology has grown rapidly in recent years. In constrast, the teaching of computer architectures has difficulties to follow such evolution. The books and the classes still use static resources which need long explanations. This dynamic becomes incompatible with the experiences desirable for most students. This work proposes the development of a computational environment (framework) to support the teaching of processing architecture, which is called MODPRO. The idea is to have modules that can be connected together forming several possible processing structures. Therefore, the professor can develop different scenarios with the students and expose in a visual way (using animation features), from basic components to more advanced processing structures. The MODPRO consists of a simulator, called SIMPRO which displays (in an animated form), step by step or in real-time, the data flow and the signal processing within the structure being studied. The SIMPRO was developed in JavaScript language, uses Cascading Style Sheets and can be accessed via web. The MODPRO also consistes in an emulator called EMUPRO, which contains the same modules of SIMPRO. Its differential is related to the fact that it has been developed entirely in hardware, using the development environment QUARTUS II from Altera. Basically, with the features of MODPRO, the students can validate, in laboratory, the frameworks and the processing structures developed during the classes. Because they are modular, both the simulator SIMPRO and the emulator EMUPRO allow the addition of new features (modules), besides allowing the teaching and the study of different processing structures / Mestrado / Engenharia de Computação / Mestre em Engenharia Elétrica

This thesis examines the reinvention of jihād ideology in twelfth‑century al‑Shām. In modern scholarship there is a tendency to speak of a revival of jihād in the twelfth century, but discussion of this revival has been dominated by study of the practice of jihād rather than of the ideology of jihād. This thesis addresses this imbalance by studying two twelfth‑century Damascene works: the Kitāb al‑jihād (Book of Jihād) of ʿAlī b. Ṭāhir al‑Sulamī (d. 500/1106), and the al‑Arbaʿūn ḥadīthan fī al‑ḥathth ʿala al‑jihād (Forty Hadiths for Inciting Jihād) of Abū al‑Qāsim Ibn ʿAsākir (d. 571/1176). Through discussion of these texts, this thesis sheds light on twelfth‑century perceptions of jihād by asking what their authors meant when they referred to jihād, and how their perceptions of jihād related to the broader Islamic discourse on jihād. A holistic approach is taken to these works; they are discussed not only in the context of the 'master narrative' of jihād, wherein juristic sources have been privileged over other non‑legal genres and corpora, but also in the context of the Sufi discourse of jihād al‑nafs, and the earliest traditions on jihād which thrived from the eighth century onwards on the Muslim‑Byzantine frontier. This thesis argues that both al‑Sulamī and Ibn ʿAsākir integrated elements from these different traditions of jihād in order to create models of jihād suited to their own political contexts, and that it is only in the context of a more nuanced appreciation of jihād ideology that their attempts can be properly understood. At the same time, this thesis argues against the model of the 'counter‑crusade', which holds that the revival of jihād began in earnest only in the middle of the twelfth century, by stressing that there was no delay between the arrival of the Franks and attempts to modify jihād ideology.

Marknaden för proteinbaserade läkemedel, de så kallade biologiska läkemedlen, är idag en industri som omsätter miljarder. Ett vanligt sätt att utveckla dessa läkemedel på är med hjälp av monoklonala antikroppar då de kan binda till sitt mål med hög specificitet. Däremot begränsas denna teknik av en lång och dyr produktion som dessutom kräver däggdjursbaserade uttrycksystem. En alternativ teknik till de monoklonala antikropparna är att använda små proteiner som enkelt kan produceras i bakterier till en låg kostnad. Dock begränsas denna metod av de små proteinernas korta cirkuleringstid i blodet. I ett tidigare projekt, har ett litet protein vid namnet ABDderived affinity ProTein (ADAPT) på cirka 7 kDa, utvecklats för att kunna binda till både humant serumalbumin (HSA) för att förlänga cirkulationstiden i blodet och Interleukin 17c (IL17c) som är ett pro-inflammatorisk cytokin. Studien visade dock att ADAPT proteinet inte samtidigt kunde binda till de båda molekylerna tillräckligt effektivt. Syftet med denna uppsats är därför att undersöka om det nämnda proteinet kan optimeras genom så kallad multimering och/eller manipulering av bindningssätet för HSA i syfte att åstadkomma en effektiv och mer långvarig cirkulationstid i blodet samtidigt som det binder sig till sitt mål, IL17c. Tio nya versioner av ADAPT proteinet har utvecklats genom att klona och transformera proteiner till en högt producerande Escherichia coli (E. coli) stam. Proteinerna har sedan producerats och renats fram. Det kunde observeras att proteinerna hade den önskade renheten för att kunna karaktäriseras. Vidare var det möjligt att se att proteinerna hade sin önskade molekylvikt och erhöll sin förväntade struktur som en alfahelix. Proteinernas smältpunkter hade förbättrats eller var liknande jämfört med det ursprungliga proteinet. Dessutom kunde alla proteiner återgå till sin ursprungliga struktur efter upphettning. Utvärderingen av proteinernas bindningskapacitet, med original proteinet som referens, visade på en ökad affinitet till sitt mål, IL17c, för två dimerer och trimeren samt en jämförbar affinitet för två av monomererna med ett manipulerat bindingssäte till HSA. Interaktion till HSA var jämförbar med den ursprungliga ADAPT molekylen för alla nya varianter förutom monomererna med ett manipulerat bindingssäte och dimeren med två manipulerat bindingssäten till HSA. Evaluering av de nya proteinernas kapacitet att binda samtidigt till HSA och IL17c visade att det var gynnsamt med en dimereiserad molekyl då det skapade en distans mellan molekylerna och dess bindningssäten. Vidare kunde det också visas att ordningen som molekylerna interagerade med varandra påverkade proteinernas simultana bindning. / The market for protein-based drugs, or the so-called biopharmaceuticals, is a multibillion-dollar industry today. In the development of protein-based drugs it is common to use monoclonal antibodies (mAbs) due to their ability to bind to its target with high specificity. However, therapeutical development of mAbs is limited by its long and expensive production in mammalian expression system. An alternative to mAbs are the so-called alternative scaffolds which are small proteins that can be produced in bacteria at lower costs. Although a drawback with the latter proteins is their short serum half-life. A small scaffold protein, ABD-Derived Affinity ProTein (ADAPT) of approximate 7 kDa was earlier engineered to obtain bispecific affinity, to Human Serum Albumin (HSA), to extend its half-life, as well as to the pro-inflammatory cytokine, Interleukin 17c (IL17c). Unfortunately, it was shown that the simultaneous binding was not efficient enough for its desired purpose. The aim with this project was therefore to investigate if the previous mentioned binder could be optimized by multimerization and/or manipulation of the HSA binding site for an efficient half-life extension. By generating ten new designs of the ADAPT variants, it was observed that the new variants had stable alpha helical structures and an improved or similar melting temperature as the original variant. The evaluation of the target binding displayed an improved affinity to the target, IL17c, for two of the dimeric versions as well as for the trimer and a comparable affinity for two of the monomers with a manipulated HAS binding site. The interaction to HSA was comparable to the original ADAPT for all binders except from the monomers with impaired HSA binding and the dimer with two impaired HSA binding sites. The evaluation of the simultaneous binding showed that it was favored by dimerization when a distance between the two molecule and their binding surfaces was added. Moreover, it could also be seen that the order of binding events had an impact on the simultaneous binding.

Under senare år har målinriktad terapi varit ett växande område inom cancerterapi som en mer målinriktad behandling än kemoterapi. Dessa behandlingar baseras främst på antikroppsbaserade läkemedel som är ganska stora och komplexa, vilket ökar den totala kostnaden för behandlingen. Därför måste man hitta en alternativ metod för både upptäckt och behandling för att hjälpa patienterna. Små affinitetsdomäner har skapats med målet att förbättra vävnadspenetrationen och samtidigt upprätthålla en hög grad av målspecificitet, vilket leder till färre biverkningar. Ett av exemplen på detta är Albumin Binding Domain-Derived Affinity Protein (ADAPT). Det har baserats på en av de albuminbindande domänerna (ABD) i streptokockproteinet G, med en storlek på 6,5 kDa. Nyligen modifierades ADAPT ytterligare för att samtidigt binda albumin och ett annat relevant målprotein av intresse, vilket tyder på en längre halveringstid i patientserum och möjliggör utveckling av nyare och terapeutiska läkemedel. I detta projekt presenteras den fjärde generationen av ADAPT-biblioteket som utformats för att ha förbättrad stabilitet. Selektioner med fagdisplay utfördes mot tre målproteiner: carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5), en biomarkör för kolorektalcancer, epithelial cell adhesion molecule (EpCAM), en markör för flera gastrointestinala karcinom och trophoblast cell-surface antigen 2 (Trop2) som är överuttryckt i trippel-negativ bröstcancer. Resultaten visar bindning till CEACAM5, EpCAM och Trop2, vilket har visats med monoklonal fag-ELISA. Bindningsaffiniteten, sekundärstrukturen hos de utvalda bindarna och den bispecifika bindningen till serumalbumin återstår att utvärdera ytterligare. Projektet visar således att de ADAPTs som valts ut mot målen CEACAM5, EpCAM och Trop2 har en enorm potential för framtida kliniska tillämpningar som syftar till utveckling av diagnostik och terapi för dessa cancerbiomarkörer. / In recent years, targeted therapy has been a growing field of cancer therapy as a more targeted treatment than chemotherapy. These treatments are primarily based on antibody-based pharmaceuticals which are quite large and complex, increasing the overall cost of the treatment. Thus, an alternative method of both detection and treatment needs to be found to aid patients. Small affinity domains have been created with the goal of enhancing tissue penetration while maintaining a high level of target specificity, leading to fewer side effects. One of the examples for these is the Albumin Binding Domain-Derived Affinity Protein (ADAPT). It has been based on one of the albumin binding domains (ABD) of the streptococcal protein G, with a size of 6.5 kDa. Recently, the ADAPTs were further modified to simultaneously bind albumin and another pertinent target protein of interest, suggesting a longer half-life in patient serum, and enabling the development of newer therapeutics. This project presents the 4th generation of the ADAPT library designed to have improved stability. Phage display selections were performed against three target proteins: carcinoembryonic antigen- related cell adhesion molecule 5 (CEACAM5), a biomarker for colorectal cancer, epithelial cell adhesion molecule (EpCAM), a marker for several gastrointestinal carcinomas and trophoblast cell-surface antigen 2 (Trop2) which is overexpressed in triple-negative breast cancer. The results demonstrate binding towards CEACAM5, EpCAM and Trop2, which has been shown by monoclonal phage ELISA. The binding affinity, secondary structure of the selected binders and bispecific binding towards serum albumin remain to be further assessed. The project thus reveals that the ADAPTs selected against the targets CEACAM5, EpCAM and Trop2 present a massive potential for future clinical applications aimed towards development of diagnostics and therapeutics for these cancer biomarkers.

Kiekviena politinės lyderystės apraiška 1990 – 1991 metais vykusio Irako – Kuveito konflikto kontekste buvo skirtinga priklausomai nuo JAV, Didžiosios Britanijos, Prancūzijos ir Irako politinių lyderių asmeninių savybių, lyderiavimo motyvų, nuo skirtingai suvokiamų atskiros visuomenės tikslų įgyvendinimo specifikos, nuo tarptautinės grupės ypatybių ir kitų socialinių, politinių, ekonominių bei kultūrinių sąlygų. Vieni politiniais lyderiais tapo dėl asmeninio patrauklumo, charizmos, kiti atsidūrė tam tikru momentu tam tikroje situacijoje, treti veikė vedini savo asmeninių ambicijų. Kaip tik todėl magistro darbo objektas yra politinių lyderių vaidmuo Irako – Kuveito konflikte. Darbo aktualumas. 1990 – 1991 metais Persijos įlankoje netrūko radikalių permainų: pagrindiniu tikslu buvo laikomas Irako prezidento Sadamo Huseino nuvertimas, o tai reiškė, jog sąjungininkai privalėjo užimti Irako sostinę Bagdadą ir kontroliuoti visus strateginius jame esančius objektus. Būtent šioje situacijoje politiniams lyderiams atsirado puiki terpė pasireikšti. Magistro darbe aiškinamasi apie galingiausių to meto valstybių verbalinį ir fizinį ryšį, jų vykdomus ar ketinamus vykdyti veiksmus. Kadangi nebuvo ir vis dar nėra jokios paprastos lyderystės formulės ar griežtos mokslinės disciplinos, kuria būtų galėję vadovautis nagrinėjami politiniai lyderiai, tai tapo akstinu pažvelgti į šią problemą iš naujo. Darbo chronologinės ribos. Pavadinime įvardintas 1990 – 1991 metų laikotarpis. Konfliktas... [toliau žr. visą tekstą] / Every political clash leaves a trace in World history. The conflict between Iraq and Kuwait in the end of XX century is not the exception. Amid growing tension between the two Persian Gulf neighbors, Saddam Hussein concluded that the United States and the rest of the outside world would not interfere to defend Kuwait. On August 2, 1990, Iraqi forces invaded Kuwait and quickly seized control of the small nation. Within days, the United States, along with the United Nations, demanded Iraq’s immediate withdrawal. U.S. and other UN member nations began deploying troops in Saudi Arabia within the week, and the worldwide coalition began to form under UN authority. On January 16, 1991, Allied forces began devastating bombing of Iraq and its forces in Kuwait. The Allied bombing sought to damage Iraq’s infrastructure so as to hinder its ability to make war while also hurting both civilian and military morale. Unfortunately, Allied air strikes and cruise missile attacks against Iraq proved more devastating than expected. When the Allied armies launched the ground war on February 23, the Iraqi occupation forces in Kuwait were already beaten. On March 3, 1991, Iraq accepted the terms of the cease-fire and the fighting ended. So the object of master‘s work has been the evaluation of the role, substantiality and particularity of political leaders during the conflict between Iraq and Kuwait in 1990 – 1991. The goal has been to point out the connection between the expression of political... [to full text]

A major goal of evolutionary developmental biology is to explore mechanisms and events underlying evolution of the myriad body plan morphologies expressed both genetically and phenotypically within the animal kingdom. Arthropods exhibit an astounding array of morphological diversity both within and between representative sub-phyla, thus providing an ideal phylum through which to address questions of body plan innovation and diversification. Major arthropod groups are recognised and defined by the distinct form and number of articulated appendages present along the antero-posterior axis of their segmented bodies. A great deal is known about the developmental genetics of limb development in the model insect Drosophila melanogaster, added to which, much comparative gene expression data and a growing body of functional genetic data is emerging for other arthropod species. Arthropod limb primordia are consistently marked by expression of the homeobox gene Distal-less (Dll), and the focus of this thesis is to compare signalling mediated by early Dll regulatory genes activity along antero-posterior and dorso-ventral embryonic axes during limb specification in Drosophila, with the activity of their orthologs in the widely disparate chelicerate, the spider mite Tetranychus urticae - interpreting new data with that available for other arthropods. Having made a detailed study of spider mite embryonic (and post-embryonic) development, to provide a basis for understanding mRNA transcription and protein activity patterns, I confirmed typical expression of Tetranychus Dll in prosomal limb primordia. I obtained limited results for the candidate antero-posterior positioning genes wingless and engrailed, although one of the two engrailed paralogs I identified is reportedly expressed in posterior segmental compartments, consistent with possible conservation of Engrailed-Wingless interactions in metameric patterning and positive regulation of Dll in arthropod limb specification. In Drosophila, wingless-dependent Dll transcription is restricted along the dorso-ventral axis by dorsal Dpp-mediated and ventral EGFR-mediated signalling gradients. Based on data from Tetranychus and other arthropods, neither dorsal nor ventral signalling regimes appear conserved outside the Drosophila system. Dll suppression in fly abdominal segments occurs due to powerful Hox (Ubx/AbdA) repression of the early Dll cis-regulatory element; this is discussed in relation to the independently evolved limbless chelicerate opisthosoma, informed by hypothetical scenarios of cis (regulatory DNA) and trans (coding sequence) evolution. Given practical difficulties and limitations encountered while working with spider mites, I offer a final assessment of the place of Tetranychus urticae as a non-model, and yet still valuable chelicerate species to consider carrying into the exciting future of evolutionary developmental biology.

Orientador: Pedro Paulo Abreu Funari / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Filosofia e Ciências Humanas / Made available in DSpace on 2018-08-22T02:29:50Z (GMT). No. of bitstreams: 1 Fredel_KarlaMaria_D.pdf: 7954004 bytes, checksum: 76882aaea349fc1be6e2234386f43a91 (MD5) Previous issue date: 2012 / Resumo: O presente estudo baseia-se na análise da cultura material (louça histórica colonial) para comparar e exemplificar as relações de gênero existentes nas sociedades oitocentistas residentes em Pelotas, Rio Grande do Sul, e Habana Vieja, Cuba. Tais relações são exemplificadas de duas maneiras: o masculino e o feminino, e a estratificação social no relacionamento senhor e escravo / Abstract: This study analyses the archaeological material culture (crockery) to compare and exemplify the gender relations in 1800's societies of Pelotas, Brazil, Rio Grande do Sul/Brazil, and Habana Vieja, in Cuba. Such relations are specified in two manners: the masculine and the feminine, and the behaviors in the master/servant social stratifications / Doutorado / Historia Cultural / Doutora em História

This study explored the influences on Doctor of Education (Ed.D.) students who have completed all required coursework for their degree, but have not finished their dissertation, in an effort to identify factors influencing degree completion. Past research documents an increased time-to-degree (TTD) for Ed.D. students, which has a negative impact on K-12 and higher education, as well as on business, government, and society. This study examined Ed.D. students enrolled at a private teaching college in northern California by use of a survey built upon the framework of Bean’s nine themes of college student retention. It analyzed demographic indicators as well as the professional and personal priorities and how these characteristics interface with the demands of completing a doctoral dissertation. The results highlight key differences between Ed.D. students and other graduate and undergraduate students to understand the reasons behind their increased TTD.

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