Snow depth variability at the plot scale: Assesment of topography and vegetation influence Abstract This master thesis deals with the evaluation of snow depth variability at the plot scale. It focuses on influence of topography and vegetation factors as slope, exposure, curvature, solar radiation and leaf area index. There is also assesment the impact of the size scale. Measurement was carried out in period of accumulation and snowmelt in winter 2014/2015 in the Krkonoše Mountains on Hanapetrova glade. To evaluate the effect of selected factors on variability of snow depth there was used multiple linear regresion and other descriptive statistical methods. The research shows that the variability of snow depth during the accumulation is greater in forest which is probably due to vegetation. The dependency was not confirmed by regression analysis. Higher variability of snow cover in the forest was also observed in the melting period. The variability of snow cover increased in the forest in general. The results show that the snow depth variability decreasses with increasing grid size. Keywords: snow accumulation, snowmelt, topography, vegetation, multivariate analysis

<p>Bacterial outer membrane vesicles (OMVs) are spherical buds of the outer membrane (OM) containing periplasmic lumenal components. OMVs have been demonstrated to play a critical part in the transmission of virulence factors, immunologically active compounds, and bacterial survival, however vesiculation also appears to be a ubiquitous physiological process for Gram-negative bacteria. Despite their characterized biological roles, especially for pathogens, very little is known about their importance for the originating organism as well as regulation and mechanism of production. Only when we have established their biogenesis can we fully uncover their roles in pathogenesis and bacterial physiology. The overall goal of this research was to characterize bacterial mutants which display altered vesiculation phenotypes using genetic and biochemical techniques, and thereby begin to elucidate the mechanism of vesicle production and regulation. One part of this work elucidated a synthetic genetic growth defect for a strain with reduced OMV production (&#916;nlpA, inner membrane lipoprotein with a minor role in methionine transport) and envelope stress (&#916;degP, dual function periplasmic chaperone/ protease responsible for managing proteinaceous waste). This research showed that the growth defect of &#916;nlpA&#916;degP correlated with reduced OMV production with respect to the hyprevesiculator &#916;degP and the accumulation of protein in the periplasm and DegP substrates in the lumen of OMVs. We further demonstrated that OMVs do not solely act as a stress response pathway to rid the periplasm of otherwise damaging misfolded protein but also of accumulated peptidoglycan (PG) fragments and lipopolysaccharide (LPS), elucidating OMVs as a general stress response pathway critical for bacterial well-being. The second part of this work, focused on the role of PG structure, turnover and covalent crosslinks to the OM in vesiculation. We established a direct link between PG degradation and vesiculation: Mutations in the OM lipoprotein nlpI had been previously established as a very strong hypervesiculation phenotype. In the literature NlpI had been associated with another OM lipoprotein, Spr that was recently identified as a PG hydrolase. The data presented here suggest that NlpI acts as a negative regulator of Spr and that the &#916;nlpI hypervesiculation phenotype is a result of rampantly degraded PG by Spr. Additionally, we found that changes in PG structure and turnover correlate with altered vesiculation levels, as well as non-canonical D-amino acids, which are secreted by numerous bacteria on the onset of stationary phase, being a natural factor to increase OMV production. Furthermore, we discovered an inverse relationship between the concentration of Lpp-mediated, covalent crosslinks and the level of OMV production under conditions of modulated PG metabolism and structure. In contrast, situations that lead to periplasmic accumulation (protein, PG fragments, and LPS) and consequent hypervesiculation the overall OM-PG crosslink concentration appears to be unchanged. Form this work, we conclude that multiple pathways lead to OMV production: Lpp concentration-dependent and bulk driven, Lpp concentration-independent.</p> / Dissertation

Arsenic (As) appears in the environment as various As species, which may vary in plant uptake and toxicity. Moreover, As exposure may vary between habitat due to availability and speciation, both of which are influenced by redox potential. To decrease As uptake, addition of silicate may be a tool. The aim of the study was to investigate how the external factors As availability, plant habitats, silicon and oxygen level, influenced the accumulation and speciation of As in plants for food and phytoremediation in a temperate region. The external factors were chosen due to their previously showed influence on As in plants. The risks with dietary As was investigated by plant As accumulation and speciation in carrot, lettuce and spinach grown in alum shale and glassworks soils, and by the influence of silicon on As accumulation in lettuce in hydroponics. Suitable plants for As phytoremediation was investigated by analysing plants from various habitats, and by the O2 influence on phytofiltration. The results showed that vegetables accumulated more As in soils with higher As extractability, and the As extractability in the rhizosphere was higher than in bulk soil. Also, the As concentration in lettuce was higher in hydroponics than in soil, but silicon reduced the accumulation of As in lettuce in hydroponics. Also, the more toxic inorganic As were the main As species detected in vegetables, compared with the less toxic organic As. For phytoremediation, the results showed a low As accumulation in emergent and terrestrial plants. Submerged plants had had a higher shoot As concentration. In general, the habitat had a major influence on the As accumulation in plants. The results also showed that the As accumulation properties in Elodea canadensis was reduced at higher O2. In conclusion, consumption of vegetables cultivated in As polluted soils can result in an elevated intake of inorganic As, and E. canadensis is a promising candidate for As phytofiltration in a temperate region. / <p>At the time of the doctoral defence the following papers were unpublished and had a status as follows: Paper 2: Manuscript; Paper 4: Manuscript; Paper 5: Manuscript.</p>

Snow accumulation patterns can be highly variable depending on terrain and wind. Knowledge of spatial variability of snow accumulation is of high relevance for mass balance modelling. By not incorporating the variability in snow cover, the estimation in mass fluxes and the surface melt are incorrectly presented, affecting the eventual estimation of for instance contribution to sea level rise. Additionally, knowledge of snow accumulation variability is essential for assessing the reliability of point-wise mass balance measurements. Using ground penetrating radar (GPR), the spatial variability of snow can be mapped with both a great spatial and temporal resolution. GPR enables tracing of summer surface melt layers, resulting in a 2D reconstruction of past snow accumulation and associated variability. GPR measurements have been done on Svalbard, during 2012, 2013 and 2014. Based on the selected 2009 summer surface in the GPR measurements, accumulation rates were estimated between 2009-2012; 2009-2013 and 2009-2014. In addition, several terrain parameters are computed by combining DEM calculations with wind direction, resulting in a sheltering index, slope and curvature. We explore relationships between the found accumulation pattern and the terrain parameters with varying wind directions. Correlations between terrain and accumulation depend on the selected wind angle, which appears to change with elevation. The results suggest that localized wind patterns prevail on the glacier and shape the snow cover. Katabatic winds form at low elevations on the glacier and are oriented in the glacier direction of approximately 20 degrees. At intermediate elevation, winds from the east-southeast regulate the accumulation pattern. On the upper parts of the glacier, the terrain is more exposed and winds from large-scale atmospheric circulation, at 240 degrees, become more important in formation of the snow accumulation pattern. Correlations are overall high, indicating a strong influence of terrain features on the accumulation distribution. No distinction can be made between the different terrain parameters and accumulation, all returning similar correlations with accumulation except for curvature, which overall returns slightly lower correlations. In addition, the results found great spatial variability in snow accumulation, underlining the importance of incorporating snow accumulation variability in glacier mass balance models. / Snow accumulation patterns can be highly variable depending on terrain and wind. Knowledge of spatialvariability of snow accumulation is of high relevance for mass balance modelling. By not incorporating the variability in snow cover, the estimation in mass fluxes and the surface melt are incorrectlypresented, affecting the eventual estimation of for instance contribution to sea level rise. Additionally,knowledge of snow accumulation variability is essential for assessing the reliability of point-wise mass balance measurements.Using ground penetrating radar (GPR), the spatial variability of snow can be mapped with both agreat spatial and temporal resolution. GPR enables tracing of summer surface melt layers, resulting in a 2D reconstruction of past snow accumulation and associated variability. GPR measurements have been done on Svalbard, during 2012, 2013 and 2014. Based on the selected 2009 summer surface in the GPR measurements, accumulation rates were estimated between 2009-2012; 2009-2013 and 2009-2014. In addition, several terrain parameters are computed by combining DEM calculations with wind direction, resulting in a sheltering index, slope and curvature. We explore relationships between the found accumulation pattern and the terrain parameters with varying wind directions.Correlations between terrain and accumulation depend on the selected wind angle, which appears to change with elevation. The results suggest that localized wind patterns prevail on the glacier and shape the snow cover. Katabatic winds form at low elevations on the glacier and are oriented in the glacier direction of approximately 20 degrees. At intermediate elevation, winds from the east-southeast regulate the accumulation pattern. On the upper parts of the glacier, the terrain is more exposed and winds from large-scale atmospheric circulation, at 240 degrees, become more important in formation of the snow accumulation pattern. Correlations are overall high, indicating a strong influence of terrain features on the accumulation distribution. No distinction can be made between the different terrain parameters and accumulation, all returning similar correlations with accumulation except for curvature, which overall returns slightly lower correlations. In addition, the results found great spatial variability in snowaccumulation, underlinin

The problem of cadmium (Cd) toxicity aggravates every year. However, the amount of plant species presenting some degree of tolerance to this metal is low. Therefore, it is of utmost importance to understand the Cd tolerance mechanisms. Two tomato cultivars exhibiting distinct levels of tolerance to Cd were investigated by exposing them to 35 &#956;m de CdCl2 for eight days. The samples (roots, stems, and leaves) were collected at 0, 2, 4, 6 and 8 days. The root length and surface area were obtained using a WinRhizo PRO 2009c image analyzer. The ICP OES method was used for the quantification of metals in the different tissues. The translocation index (TrI), metal uptake and Cd distribution nutrient absorbed (NA), and nutrient efficiency (NUE) were calculated. The plant material used for stress indicators and proteomics analysis were collected in liquid nitrogen. Lipid peroxidation and H2O2 contents were obtained by spectrometry. For the proteomics data the gel-free method was used. The stress indicators showed the variation in both cultivars, evidencing the stress caused by the Cd. Chlorosis and necrotic spots were observed in plants exposed to Cd less significant way in the first days of exposure and more evident in the less tolerant cultivar (TTO) in the last days. The growth reduction can be observed in both cultivars, more evident in TTO. Regarding the Cd distribution, the two cultivars showed similar behavior, with the highest percentage of Cd in the roots. The Cd absorption could interfere with both cultivars in the accumulation absorption and efficiency in the use of most nutrients assessed. In general, the Cd interference was more evident at TTO than at YOSH. Highest percentages of TrI were also observed in TTO cultivar. Identification and evaluation of the differentially accumulated protein (DAP) in the two cultivars roots it is possible to observe that in the presence of Cd the cultivar TTO generates a more significant alteration in the amplitude of the accumulation and the number of proteins. These results suggest that the cultivar, which presents a higher degree of tolerance, has some form to make the Cd unavailable or a mechanism that makes the Cd transportation to the leaves more difficult. / O problema da toxicidade do cádmio (Cd) agrava-se a cada ano. No entanto, a quantidade de espécies de plantas que apresentam algum grau de tolerância a este metal é baixa. Portanto, é de suma importância entender os mecanismos de tolerância do Cd. Com esse intuito duas cultivares de tomate (TTO e YOSH) exibindo níveis distintos de tolerância ao Cd foram investigadas, expondo-as a 35 &#956;m de CdCl2 por oito dias. As amostras (raízes, caules e folhas) foram coletadas aos 0, 2, 4, 6 e 8 dias. O comprimento da raiz e área de superfície foram obtidos usando um analisador de imagem WinRhizo PRO 2009c. O método ICP OES foi utilizado para a quantificação de metais nos diferentes tecidos. O índice de translocação (Trl), a absorção de metal e a distribuição de Cd dos nutrientes absorvidos (NA) e eficiência de nutrientes (NUE) foram calculados. O material vegetal usado para indicadores de estresse e análise proteômica foi coletado em nitrogênio líquido. A peroxidação lipídica e o teor de H2O2 foram obtidos por espectrometria. Para os dados proteômicos, foi utilizado o método livre de gel. Os indicadores de estresse mostraram a variação nas duas cultivares, evidenciando o estresse causado pelo Cd. A clorose e manchas necróticas foram observadas em plantas expostas ao Cd de maneira menos significativa nos primeiros dias de exposição e mais evidentes no cultivar menos tolerante (TTO) nos últimos dias. A redução do crescimento pode ser observada em ambas as cultivares, mais evidente no TTO. Quanto à distribuição de Cd, as duas cultivares apresentaram comportamento semelhante, com o maior percentual de Cd nas raízes. A absorção de Cd pode interferir nas duas cultivares na absorção, acúmulo e eficiência no uso da maioria dos nutrientes avaliados. Em geral, a interferência do Cd foi mais evidente no TTO do que no YOSH. As maiores porcentagens de TrI também foram observadas na cultivar TTO. Identificação e avaliação da proteína diferencialmente acumulada (DAP) nas raízes das duas cultivares é possível observar que na presença de Cd a cultivar TTO gera uma alteração mais significativa na amplitude do acúmulo e no número de proteínas. Estes resultados sugerem que a cultivar, que apresenta um maior grau de tolerância, tem alguma forma de tornar o Cd indisponível ou um mecanismo que dificulte o transporte de Cd para as folhas.

O caquizeiro (Diospyros kaki L.) é uma planta caducifólia pertencente à família Ebenaceae, tendo como centro de origem a região central da China. Entre os estados brasileiros, São Paulo é o que apresenta maior produção de caquis com 49.727 t. A cercosporiose (Pseudocercospora kaki), também conhecida como mancha angular, é a principal doença foliar da cultura. Alguns autores atribuem à cercosporiose a queda precoce de folhas, maturação antecipada dos frutos e a redução da produção na safra seguinte. Entretanto, não existem trabalhos com o patossistema P. kaki - D. kaki que deem suporte a essas pressuposições. Diante disso, os objetivos desse trabalho foram comparar a susceptibilidade de quatro cultivares de caquizeiro e avaliar o efeito da cercosporiose na fisiologia da planta. Os experimentos foram conduzidos em condições naturais de campo, com as cultivares Fuyu, Rama Forte, Taubaté e Giombo, por serem as mais cultivadas no estado de São Paulo. Nos experimentos desenvolvidos buscou-se conhecer o grau de resistência das quatro cultivares à cercosporiose, avaliando a incidência, severidade e queda de folhas ao longo de dois ciclos de cultivo, assim como avaliar o controle químico na redução da doença em cvs. Taubaté e Rama Forte e, consequentemente, no acúmulo de reservas na raiz e na qualidade dos frutos colhidos. Foram avaliadas também os danos na fotossíntese em folhas das quatro cultivares com diferentes intensidades de cercosporiose. Como resultados pode-se constatar, que a cv. Fuyu é a mais susceptível à cercosporiose, seguida com resistência média \'Giombo\' e \'Rama Forte\' e com maior resistência a cv. Taubaté. A intensidade da cercosporiose, para severidades da doença de até 7% não influencia na queda de folhas. O controle químico reduziu a intensidade de doença nas cvs. Taubaté e Rama Forte, entretanto, entre as intensidades de cercosporiose avaliadas em plantas tratadas e não tratadas, não há redução no acúmulo de reservas no sistema radicular, assim como na qualidade físico-química dos frutos colhidos. Mesmo a cercosporiose afetando a fotossíntese, no processo fotoquímico, tanto na área lesionada como na área adjacente à lesão, a intensidade da doença observada no campo não foi suficiente para causar redução da produção à cultura. / The persimmon (Diospyros kaki L.) is a deciduous plant of the botanical family Ebenaceae, native to central China. Among the Brazilian states, São Paulo has the highest production of persimmon with 49,727 ton/year. The angular leaf spot of persimmon (Pseudocercospora kaki), also known as cercosporiosis, is the main leaf disease of the crop. Some authors attribute to the cercosporiosis the premature defoliation, early maturation of the fruits and the reduction of the yield in the next crop cycle. However, there are no previous studies with the Pseudocercospora kaki pathosystem that support these hypotheses. Therefore, the objectives of this study were to compare the susceptibility of four persimmon cultivars and evaluate the effect of angular leaf spot on plant physiology. A field experiment was conducted with Fuyu, Rama Forte, Taubaté and Giombo cultivars, the four most cultivated in the state of São Paulo. The degree of resistance to cercosporiosis was determined by the incidence and severity of the disease and by defoliation of the plants in two crop cycles. Chemical spraying for the disease reduction was also evaluated in cvs. Taubaté and Rama Forte. The reduction of the carbohydrate reserve accumulation in the roots and the quality of the fruits harvested were tested. The photosynthesis reduction was also evaluated in leaves of the four cultivars with different intensities of cercosporiosis. The Fuyu cultivar was the most susceptible to cercosporiosis, followed by moderate resistance of \'Giombo\' and \'Rama Forte\' and high resistance of cv. Taubaté. The intensity of cercosporiosis, for disease severity up to 7% does not influence leaf drop. Though the chemical control reduced the disease intensity in cvs. Taubaté and Rama Forte, treated and untreated plants showed no differences in the reserves accumulation in the roots, as well as in the physicochemical quality of the harvested fruits. Even though cercosporiosis affects photosynthesis in the photochemical process on both the necrotic and remaining green leaf area, the intensity of the disease observed in the field was not enough to cause yield reduction to the culture.

Environmental contamination with heavy metals, especially of soils, has been a continuousproblem worldwide since the beginning of the industrial revolution. Heavy metal emissionshave increased continuously since 1900 and the metals accumulate in the environment. Pulpand paper mill factories, and factories which produce sulphuric acid emit heavy metals,among others lead (Pb), copper (Cu) and zinc (Zn). Results from previous studies showedthat the soils near factories had higher concentrations of these pollutants than the controlplace. In this study I want to determine the effects of industrial heavy metal emissions of onehistoric and one present industry on nearby soil heavy metal concentrations.Samples were taken near one historically heavily contaminated site, the old sulphite factoryin Rydöbruk (1897-1944) and near the present Stora Enso paper mill in Hyltebruk. As acontrol site, I compared these with Rocknen nature reserve area.At each site I took 6 soil samples, representing the top 15-20 cm of soil and organic material.Samples were analysed with an Atomic Absorption Spectrometer and the concentrations oflead, copper and zinc were determined per ashed dry material.I analyzed the samples at the laboratory using the guideline from the Swedish StandardInstitute for water analysis.The concentrations of the soil samples showed no significant difference between the naturereserve area and the industrial sites (One-way ANOVA). This was due to very variableorganic content within and between the sites.Organic content of the samples has a significantly effect on the metal concentrations for Pband Cu (Linear Regression test). In conclusion, metals can accumulate in the vegetation, andin case of Pb and Cu there is a correlation between their concentration and the amount oforganic matter. Therefore in future studies it is best if soil with very high organic content orvegetation is used to determine if forest soil near industries are affected by past or presentindustrial emissions of heavy metals.

Os estudos realizados em diversas partes do mundo sobre a utilização de pesticidas e os impactos que estes podem causar ao ambiente são uma das ações prioritárias para o desenvolvimento da agricultura, principalmente para aqueles pesticidas que são persistentes ao meio biótico e abiótico, como o inseticida heptacloro, que apesar de seu uso restrito para tratamento de madeira, ainda é muito usado no Brasil clandestinamente para outros fins. A maioria dos estudos realizados até agora sobre biomagnificação envolve o meio terrestre e uma pequena porcentagem desses é direcionada para o meio aquático e tem sido realizada em modelos de campo. O objetivo deste projeto foi determinar a CL50 96 horas para peixes a acumulação, biomagnificação e depuração do heptacloro em um modelo de sistema aquático. Como não existe nenhuma norma que defina o efeito cumulativo através dos níveis tróficos, pretendeu-se com esta pesquisa estimar a biomagnificação e depuração do heptacloro. Este estudo foi realizado em laboratório, em um modelo dividido em compartimentos individualizados, no qual somente as algas (Pseudokirchneriella subcapitata) foram submetidas por um período de 7 dias ao heptacloro, obtendo-se uma concentração final de 0,10 x 10-5µg heptacloro/µg algas, as quais posteriormente serviram de alimento para os microcrustáceos (Daphnia magna) durante 9 dias, que ao final concentraram 0,12 x 10-5 µg heptacloro/µg organismos. Por último, os peixes (Danio rerio) foram alimentados com microcrustáceos, no período de 5 dias, alcançando uma concentração final de 0,62 x 10-8 µg heptacloro/µg organismos. Nos últimos 9 dias, durante o período de depuração, os peixes foram alimentados em dias intercalados com microcrustáceos e ração sem contaminação com o produto, atingindo uma concentração final de 0,25 x 10-8 µg heptacloro/µg organismos. Nos testes realizados com esses três organismos, observou-se que a concentração ficou maior conforme o nível dentro do modelo proposto era mais alto, comprovando assim a hipótese do poder de bioacumulação, biomagnificação e depuração do heptacloro. / The studies carried out in different parts of the world about the pesticides use and the impacts that they can cause to the environment, are one of the priority actions for the agriculture development, mainly for those pesticides that are persistents in the biotic and abiotic environment, such as the heptachlor insecticide, which is still illegally used for others purposes in Brazil, although its use is limited for the wood treatment. The majority of the studies carried out until now about biomagnification involves the terrestrial environment and a small percentage of these studies are directed for the aquatic environment, and this small percentage has been carried out in field models. The aim of this project was to determine the LC50 96 hours for fish, accumulation, biomagnifications, and depuration of heptachlor in an aquatic system model and, as there isn?t any norm that defines the cumulative effect through trophic levels, it was claimed with this research to esteem the heptachlor biomagnification and depuration. This study was carried out in a laboratory, in a model divided in single chambers, in which the algae (Pseudokirchneriella subcapitata were submitted during 7 days to the heptaclhor, getting a final concentration of 0.10 x 10-5µg heptaclhor/µg algae, which was later served as food for the microcrustaceans (Daphnia magna) were submitted during 7 days to the heptaclhor, getting a final concentration of 0.10 x 10-5Mµg heptaclhor/µg algae, which was later served as food for the microcrustaceans (Daphnia magna) were fed with microcrustaceans, in the period of 5 days, reaching a final concentration of 0.62 x 10-8 µg heptaclhor/µg organisms. In the last 9 days, during the period of depuration, the fish were fed in days intercalated with microcrustaceans and ration without contamination with the product, reaching a final concentration of 0.25 x 10-8 µg heptacloro/µg organisms. In tests carried out with these three organisms, it was observed that the concentration was bigger as higher the level inside of the considered model, thus proving the hypothesis of the bioaccumulation, biomagnification, and depuration power of heptachlor.

A metrópole de São Paulo passa por profundas transformações desde a década de 1990. O chamado vetor sudoeste se elaborou como a novacentralidade econômica do país. Esse processo se remete à reprodução do espaço urbano como condição, meio e produto da acumulação de capital. A potência desse fenômeno e dessa condição é relativamente nova. A história da concentração geográfica do capitalna cidade de São Paulo favoreceu sempre a abertura de novas oportunidades de investimentos in loco, mas a intensidade do processo aumenta exponencialmente quando um movimento de concentração começa a dar lugar à centralização geográfica do capital.A partir daí, a concentração se torna, ela mesma, a condição de reposição de novas oportunidades de investimentos na centralidade constituída, numa dinâmica que consome configurações espaciais previamente capitalistas. Trata-se de uma acumulação especificamente urbana. No Brasil, essa dinâmica espacial do capital não foi tão significativa até a década de 1990. A hipótese que explica esse descompasso no caso brasileiro reconhece a prioridade do processo de produção sobre a reprodução do espaço urbano. Isso significa que a urbanização se estendeu no tempo porque pôde se estender no espaço. A urbanização expandida do território brasileiro foi aqui objeto da ação de um Estado comprometido com a realização do capital e assim absorveu lucrativamente os excedentes frequentemente gerados na economia brasileira. Com a crise do Estado desenvolvimentista e com as condições apresentadas nos grandes centros nacionais, o processo de valorização exigiu mais do que se podia conseguir com a manutenção de uma enfraquecida dinâmica expansionista. Aqui o Estado se reorganiza e cria novos instrumentosque o permitem atuar na escala metropolitana. A produção de novas centralidades, como momento da reprodução do espaço urbano, e a centralização geográfica diminuem a importância das dinâmicas expansionistas na realização do capital. A intensificação geográfica do capital, na era das finanças mundializadas, passa a compor o centro das estratégias de acumulação. / The metropolis of São Paulo has undergone profound changes since the 1990s. The so-called southwest vector has established itself as the new economic centre in the country. This process relates to the reproduction of the urban space as a condition, means and product of capital accumulation. The strength of this phenomenon and of this condition is relatively new. The history of the geographical concentration of capital in the city of São Paulo has always privileged new investment opportunitiesin loco, however, whenever the movement of concentration starts to give way to geographic centralization of capital, the process intensifies exponentially. At this point, the concentration itself becomes the condition for the reestablishment of new investment opportunities in the recently instituted centrality: a dynamics that consume previous capitalist spatial configurations. And thisis a specifically urban accumulation. In Brazil, the spatial dynamics of capital had not been significant until the 1990s decade. The hypothesis used to explain the mismatch in the case of Brazil recognizes the priority of the production process over thereproduction of the urban space. That means that the urbanization has extended in time just because it could extend in space. The expanded urbanization of the Brazilian territory has been the result of a state policy committed to capital development, therefore, it has absorbed profitably the surpluses frequently generated in the Brazilian economy. Due to the crisis of developmentalism and the conditions prevailing in the major national centers, the valorization process required more than could be achieved with the maintenance of a weak expansionist dynamics. From this point on, the state reorganizes itself and creates new instruments that permit action at the metropolitan scale. The production of new centers, as a dimension of the reproduction of urban space, as well as the geographical centralization, diminish the importance of the expansionist dynamics for capital. In the age of global finance, the geographical intensification of capital becomes the mainstay of accumulation strategies.

O crisântemo cultivado em vaso é um dos produtos floríferos mais vendidos na floricultura brasileira, possuindo grande diversidade de cores, formas e tamanhos de inflorescências, além de possuir alta durabilidade após a colheita. Para tanto, é necessário o atendimento das exigências nutricionais da planta para possibilitar expressar seu potencial ornamental. Os objetivos deste trabalho foram analisar o crescimento da planta e caracterizar a marcha de absorção de nutrientes no cultivo em vaso do crisântemo, variedade cultivada ,,Fine Time\". O experimento foi realizado na estufa climatizada do Setor de Floricultura do Colégio Politécnico da UFSM, Santa Maria - RS, no período de setembro a dezembro de 2012. O delineamento experimental foi inteiramente casualizado com oito tratamentos e seis repetições. As plantas foram coletadas e analisadas em intervalos que variaram de 7 a 14 dias dependendo da fase de desenvolvimento da planta. Os parâmetros de crescimento avaliados foram: comprimento das hastes, folhas e de raízes; número de folhas, raízes, brotações, botões florais e de inflorescências; largura e área das folhas; massa de matéria seca das folhas, raízes; botões florais; inflorescências, hastes e das plantas. Em relação aos nutrientes foi analisado teor, acúmulo e marcha de absorção de N, P, K, Ca, Mg, S, B, Cu, Fe, Mn e Zn nas raízes, folhas, hastes, botões florais e inflorescências. Os parâmetros comprimento das hastes e raízes, número de folhas e raízes, massa de matéria seca de planta, haste e raiz foram crescentes em função do tempo, tendendo a estabilidade no final do ciclo produtivo. Os parâmetros comprimento e largura das folhas decrescem em função do tempo de cultivo. A ordem decrescente de acúmulo de massa de matéria seca dos órgãos foi: folhas > hastes > raízes > inflorescências > botões florais. A necessidade de N, P, S e Cu é semelhante durante a fase vegetativa e de florescimento. A necessidade de K, Mn e Zn nas plantas é maior na fase de florescimento. A necessidade de Ca, B, Fe e Mg nas plantas é maior na fase vegetativa. A necessidade dos macronutrientes durante a fase vegetativa e de florescimento da planta segue, respectivamente, a seguinte ordem de grandeza: Ca > Mg > N > S > P > K; e K > P > S > N > Mg > Ca. A necessidade dos micronutrientes durante a fase vegetativa e de florescimento da planta segue, respectivamente, a seguinte ordem decrescente de grandeza: B > Fe> Cu > Zn > Mn; e Mn > Zn > Cu > Fe > B. A sequência decrescente do acúmulo de nutrientes nas plantas de crisântemo em função do tempo foi: N > K > P > Ca > S > Mg > Fe > Mn > B > Zn > Cu. / The chrysanthemum cultivated in pot is one of the best seller floristic products in the Brazilian floriculture, having high colors diversity, forms and sizes of inflorescences, besides present high post-harvest durability. Therefore, it is necessary to attend the plant nutrition requirement for enabling to express its ornamental potential. The objectives of this research were to evaluate the plant growth and to characterize the nutrients uptake curve of chrysanthemum, cv. ,,Fine Time\", cultivated on pot. The experiment was carried out at greenhouse of the Floriculture Section, Polytechnic College, Federal University of ,,Santa Maria\" (UFSM), Santa Maria, ,,Rio Grande do Sul\" state, Brazil, from September to December 2012. The statistical design was complete randomized with eight treatments and six replications. The plants were sampled and analyzed in intervals of 7 to 14 days according to the plant development stage. The evaluated growth parameters were: length of stems, leaves and roots; number of leaves, roots, sproutings, buds and inflorescences; width and area of leaves; dry matter of leaves, roots, buds, inflorescences, stems and plants. In relation to the nutrients content, accumulation and uptake curve, the N, P, K, Ca, Mg, S, B, Cu, Fe, Mn and Zn on the roots, leaves, stems, buds and inflorescences were evaluated. The parameters stem and root length, number of leaves and roots, dry matter of plant, stems and roots were crescent as function of time, presenting a tendency of stability at end of the crop cycle. The parameters length and width of leaves decrease as function of cultivation time. The decrescente order of organ dry matter accumulation was: leaves > stems > roots > inflorescences > buds. The needs of N, P, S and Cu are equal during the vegetative phase and flowering. The needs of K, Mn and Zn in the plants are higher on the flowering phase. The plant demand of Ca, B, Fe and Mg is higher on the vegetative phase. The needs of macronutrients during vegetative phase and flowering obey, respectively, the following order: Ca > Mg > N > S > P > K and K > P > S > N > Mg > Ca. The needs of micronutrients during vegetative phase and flowering obey, respectively, the following decrescent order: B > Fe> Cu > Zn > Mn and Mn > Zn > Cu > Fe > B. The decrescent sequence of nutrient accumulation on the chrysanthemum plants as function of time was: N > K > P > Ca > S > Mg > Fe > Mn > B > Zn > Cu.