This academic work deals with children who are school age or younger school age and their interest in skiing and snowboarding. It tries to expose the reason of decreasing attendance at ski courses offered by schools. The research made by questionnaire shows that most of the children attending lower grades of elementary schools do some sport. They would also like to attend the ski course but they often listed finances as the obstacle. The results of interviews made with teachers of elementary schools and high schools display that only the children who had experienced winter sports either before entering elementary school or soon after entering school want to participate. This work considers as important placing the ski courses at the first grade of the elementary schools. The number of children interested in winter sports would increase. More of them would consider participating at ski courses that help raising the physical and mental condition. Keywords: younger school age, physical activities, snowboarding, skiing

This thesis deals with optimization of capillary electrophoresis conditions for the separation of unreacted substrate and products (cellobiose, glucose) generated by the enzymatic cleavage of cellotriose with enzyme cellobiase. The optimal separation electrolyte was shown to be a solution containing 30 mmol·dm-3 sodium hydroxide with addition of 1.0 mmol·dm-3 sodium phosphate and 1.0 mmol·dm-3 sodium phosphate dibasic (pH = 12.38). With this separation electrolyte calibration curves and repeatability were measured and the limits of detection and quantification were determined. The relative standard deviations of peak areas ranged up to 6.1 % and they did not exceed 0.2 % for the migration time. Limits of detection were determined in the range from 0.020 to 0.026 mmol·dm-3 and limits of quantification from 0.066 mmol·dm-3 to 0.085 mmol·dm-3 . Subsequently the enzyme reaction was monitored in offline setup. Furthermore, the offline procedure was automated and the relative standard deviation of cellodextrines after 30 minutes of hydrolysis was ranging up to 13.0 % of glucose and cellotriose and 3.0 % cellobiose using this automated method, which makes it suitable for semiquantitative and comparative measurements. The last part of the experiment was to carry out enzymatic reaction in online setup....

Lifestyle diabetic 2nd type Aims: The aim of this thesis is to outline alternatives how to improve medical condition of patients who suffer from diabetes mellitus 2nd type by non- pharmacological treatment. Another target is to create and application an intervention exercise program. It is also necessary to evaluate measured physical parameters plus to compare results before the beginning and after the termination of the intervention. Last but not least is important suggestion of recommendations for the future therapy of patients with NIDDM. Methods: In this work, there are stated contemporary information of relationship between physical activity and diabetes of the second type of a patient. Literature research was used to get this knowledge. Based on these facts and available options, the experiment related to training program was designed. This observation takes 20 weeks. Results: The controlled diabetic patient underwent the intervention program without significant complications and in full extent. Non-pharmacological treatment focused primarily on increasing physical activity performed by walking and practicing on a exercise bike, on the contrary, dietary regimen was not changed or a adjusted. The results after intervention showed improvements in physical state of a patient such as reduction in...

Title: Electroencephalographic correlates of prolonged locomotor performance of professional musicians Summary: The aim of this work is to verify the presence of alpha activity in the electroencephalographic recording during prolonged (20 minute) violin play and compare its morphological and topical parameters with the native EEG record before and after the performance. Research sample consisted of five professional violinist in the age range of 25- 60 years. The results showed the occurrence of alpha activity for four of five probands, in one case with a very low incidence. There has been also demonstrated changes in the distribution of alpha activity from parietooccipital areas before the preformance to central areas during the play and immediately after finishing. All probands showed increased amplitude of the alpha activity immediately after finishing. The obtained results confirm the changes of morphology and the changes of topic alpha activity during cognitive activities and at the onset of central fatigue during physical aktivity described in literature. These changes were demonstrated by increasing the amplitude of alpha activity and the shift from parietooccipital areas to central areas. Keywords: EEG, alpha aktivity, violin performance, brain mapping

Title: Analysis of customer satisfaction in the leisure center. Objectives: This thesis is focused on leisure center Offpark. The main goal of this thesis is to find out satisfaction or dissatisfaction with the service in the leisure center. Offpark offer for its customer leisure activities - services which the thesis analyze and improve their individual aspects. The thesis compares the expected and the real value of individual aspects. This comparison will help deduce possible changes to improve the quality of services by major deficiency. The supporting aim is analyzing the thoughts and opinions of staff and a comparison with the ideas and opinions of real visitors. This comparison should discover distinction in opinions and views of visitors and instructors. The resulting differences in these two groups showed differences in understanding of the situation made by the visitors and the instructors, which was in some cases fundamental distinction. Method: A survey of service quality was investigated by using Servqual questionnaire, which was distributed by e-mail address database of visitors leisure center Offpark. The research was carried out at the end of season 2012, when the research was presented to visitors as a tool to improve the quality of service to the next year. The basic idea of this...

Title: Possibilities of physiotherapy in pregnancy Objectives: The aim of this study is to create a comprehensive overview of current knowledge concerning the issue of pregnancy from the point of view of a physiotherapist. Literature resources will be collected in order to provide information about the frequent difficulties of pregnant women in the context of the musculoskeletal system and their possible treatment by physiotherapy and also about physical activity during pregnancy. Methods: This study is a literature review. It is a theoretical study primarily based on foreign sources found in electronic databases such as Pubmed, EBSCO, OvidSP, Google Scholar and ProQuest. 139 foreign studies related to pregnancy were included, of which 133 were published between the years 2000 and 2015. Next 6 studies published from 1978 to 1999 were found in the primary sources. A combination of the following keywords was used to search the databases: pregnancy, physical therapy, physiotherapy, physiological changes, low back pain, pelvic girdle pain, pelvic floor muscles, musculoskeletal problems, foot arch, exercise, physical activity, gestational diabetes mellitus, preeclampsia. Results: There are a lot of studies concerning to this issue, but in many cases they are too heterogeneous to draw clear conclusions....

Title: Socio-economic aspects of physical activities for seniors in Prague Objectives: The aim of study was to find out what are the socioeconomic aspects of walking and other physical activities Prague's population of seniors. Specifically find answers to questions, whether regular walk in this population affect the quality of life in old age, if those people bring motion standard from earlier stages of life, what are their motives for periodic walking, if they operate other physical activities than walking and whether is there a coherent categories of pedestrians. Methods: In this thesis were used quantitative and qualitative research methods. The source of quantitative analysis was the questionnaire WHOQOL OLD with a complementary inquiry on walking. The sample consisted of 156 respondents and was divided equally between pedestrians and non-pedestrians. There were used methods of descriptive statistics and statistical hypothesis testing. For qualitative analysis was with a part-respondents (pedestrians) made an interview, investigating their socioeconomic status, the motives for carrying out walking and other activities, and also was made their segmentation. Results: Statistically significantly higher quality of life (at 95% confidence level) in the domains of Independence, Filling, Nearby...

The aim of this work is to create an educational DVD with aerobics classes for beginners and intermediate exercisers. The DVD is designed for children and adults who want to work out at home in their free time, for novice teachers of aerobics, to liven up their lessons, and finally for heads of various sports clubs teachers who would like to include aerobics to their lessons. In the theoretical part the characteristic of aerobics is introduced, its effect on human health, and particular types of aerobics, including racing. In conclusion of the theoretical part basic steps of aerobics are described. In addition, there is also English terminology and explanation of teaching methods that are used in aerobics classes. The practical part is devoted to the learning DVD. The introduction describes the preparation for filming, followed by a transcript of choreography and stretching exercises that are used in the video, and also instructions on how to start with an exercise and what to avoid. The conclusion of the practical part is focused on a review of the DVD. The evaluation was carried out using a questionnaire survey, which was attended by a total of 60 respondents. It examined six different criteria that respondents assessed with grade 1 to 5. Overall, the DVD was evaluated by 61,7% respondents as...

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Jana Kroupová Supervisor: PharmDr. Anna Jirkovská, Ph.D. Title of diploma thesis: The effect of the proteasome inhibition of the antiproliferative effect of anthracycline antibiotics Anthracycline antibiotics (daunorubicin, doxorubicin) belong to the most effective antitumor drugs. In current clinical practice they are used mostly in the combinations with either "classical" or new targeted antitumor drugs. The proteasome inhibitors (bortezomib and carfilzomib) are also viewed as a part of new "targeted" antitumor drugs. The proteasome is a multienzyme complex in eukaryotic cells which is responsible for intracellular degradation of proteins. The proteasome inhibitors have been largely used in the therapy of multiple myeloma, but their potential has been also studied in the case of other malignancies. Their use in the combination with anthracyclines could be a possible alternative in the therapy of some tumor illnesses, but the effect of combination of anthracyclines and proteasome inhibitors on tumor cells have not been sufficiently explained. The anthracycline therapy is also accompanied by serious adverse side effect - the cardiotoxicity, which potential could be influenced by the...

BACKROUND: A school prevention is a very discussed and continuosly developing area in Czech Republic. Its quality and effective providing are the keys for preclusion of hazardous behavior development of children and adolescents. It is essential the preventive programs to be provided in accordance with the latest research findings in order to serve the purpose. AIMS: This diploma thesis aimed at two basic targets. The first one was to find out if any and which preventive activities took place at schools participating in a study (which forms of these preventive activities exist, what is their time allocation and a way of their assurance) during the termtime 2014/2015. The second target was to define differences between schools, which the preventive activities were executed in terms of a normal a.k.a school preventive program (a control group of schools) at and between schools, which in addition the program Unplugged took place in its basic or extended version (an experimental group of schools) at. METHODS: To obtain the data a quantitative method of online questionnaire was chosen, via Qualtrics database. The addressed schools and school institution were selected for the study by a randomization method. The retrieved data was evaluated via a basic descriptive statistics. RESULTS: The retrieved...